Molecular plant pathology最新文献_第5页

The root-knot nematode effector MiEFF12 targets the host ER quality control system to suppress immune responses and allow parasitism. 根结线虫效应子MiEFF12以宿主ER质量控制系统为靶标，抑制免疫反应，实现寄生。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-07-01 DOI: 10.1111/mpp.13491

Salomé Soulé, Kaiwei Huang, Karine Mulet, Joffrey Mejias, Jérémie Bazin, Nhat My Truong, Junior Lusu Kika, Stéphanie Jaubert, Pierre Abad, Jianlong Zhao, Bruno Favery, Michaël Quentin

{"title":"The root-knot nematode effector MiEFF12 targets the host ER quality control system to suppress immune responses and allow parasitism.","authors":"Salomé Soulé, Kaiwei Huang, Karine Mulet, Joffrey Mejias, Jérémie Bazin, Nhat My Truong, Junior Lusu Kika, Stéphanie Jaubert, Pierre Abad, Jianlong Zhao, Bruno Favery, Michaël Quentin","doi":"10.1111/mpp.13491","DOIUrl":"10.1111/mpp.13491","url":null,"abstract":"Root-knot nematodes (RKNs) are microscopic parasitic worms able to infest the roots of thousands of plant species, causing massive crop yield losses worldwide. They evade the plant's immune system and manipulate plant cell physiology and metabolism to transform a few root cells into giant cells, which serve as feeding sites for the nematode. RKN parasitism is facilitated by the secretion in planta of effector molecules, mostly proteins that hijack host cellular processes. We describe here a conserved RKN-specific effector, effector 12 (EFF12), that is synthesized exclusively in the oesophageal glands of the nematode, and we demonstrate its function in parasitism. In the plant, MiEFF12 localizes to the endoplasmic reticulum (ER). A combination of RNA-sequencing analysis and immunity-suppression bioassays revealed the contribution of MiEFF12 to the modulation of host immunity. Yeast two-hybrid, split luciferase and co-immunoprecipitation approaches identified an essential component of the ER quality control system, the Solanum lycopersicum plant bap-like (PBL), and basic leucine zipper 60 (BZIP60) proteins as host targets of MiEFF12. Finally, silencing the PBL genes in Nicotiana benthamiana decreased susceptibility to Meloidogyne incognita infection. Our results suggest that EFF12 manipulates PBL function to modify plant immune responses to allow parasitism.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11222708/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141498446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The trichothecene mycotoxin deoxynivalenol facilitates cell-to-cell invasion during wheat-tissue colonization by Fusarium graminearum. 单端孢霉烯霉菌毒素脱氧雪腐镰刀菌醇（deoxynivalenol）在禾谷镰刀菌的小麦组织定殖过程中促进了细胞间的侵染。

IF 4.9 1区农林科学

Molecular plant pathology Pub Date : 2024-06-01 DOI: 10.1111/mpp.13485

Victoria J Armer, Martin Urban, Tom Ashfield, Michael J Deeks, Kim E Hammond-Kosack

{"title":"The trichothecene mycotoxin deoxynivalenol facilitates cell-to-cell invasion during wheat-tissue colonization by Fusarium graminearum.","authors":"Victoria J Armer, Martin Urban, Tom Ashfield, Michael J Deeks, Kim E Hammond-Kosack","doi":"10.1111/mpp.13485","DOIUrl":"10.1111/mpp.13485","url":null,"abstract":"Fusarium head blight disease on small-grain cereals is primarily caused by the ascomycete fungal pathogen Fusarium graminearum. Infection of floral spike tissues is characterized by the biosynthesis and secretion of potent trichothecene mycotoxins, of which deoxynivalenol (DON) is widely reported due to its negative impacts on grain quality and consumer safety. The TRI5 gene encodes an essential enzyme in the DON biosynthesis pathway and the single gene deletion mutant, ΔTri5, is widely reported to restrict disease progression to the inoculated spikelet. In this study, we present novel bioimaging evidence revealing that DON facilitates the traversal of the cell wall through plasmodesmata, a process essential for successful colonization of host tissue. Chemical complementation of ΔTri5 did not restore macro- or microscopic phenotypes, indicating that DON secretion is tightly regulated both spatially and temporally. A comparative qualitative and quantitative morphological cellular analysis revealed infections had no impact on plant cell wall thickness. Immunolabelling of callose at plasmodesmata during infection indicates that DON can increase deposits when applied exogenously but is reduced when F. graminearum hyphae are present. This study highlights the complexity of the interconnected roles of mycotoxin production, cell wall architecture and plasmodesmata in this highly specialized interaction.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11178975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141321247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel xylanase from a myxobacterium triggers a plant immune response in Nicotiana benthamiana. 一种来自霉菌的新型木聚糖酶会引发烟草中的植物免疫反应。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-06-01 DOI: 10.1111/mpp.13488

Yuqiang Zhao, Kun Yang, Yanxin Wang, Xu Li, Chengyao Xia, Yan Huang, Zhoukun Li, Cancan Zhu, Zhongli Cui, Xianfeng Ye

{"title":"A novel xylanase from a myxobacterium triggers a plant immune response in Nicotiana benthamiana.","authors":"Yuqiang Zhao, Kun Yang, Yanxin Wang, Xu Li, Chengyao Xia, Yan Huang, Zhoukun Li, Cancan Zhu, Zhongli Cui, Xianfeng Ye","doi":"10.1111/mpp.13488","DOIUrl":"10.1111/mpp.13488","url":null,"abstract":"Xylanases derived from fungi, including phytopathogenic and nonpathogenic fungi, are commonly known to trigger plant immune responses. However, there is limited research on the ability of bacterial-derived xylanases to trigger plant immunity. Here, a novel xylanase named CcXyn was identified from the myxobacterium Cystobacter sp. 0969, which displays broad-spectrum activity against both phytopathogenic fungi and bacteria. CcXyn belongs to the glycoside hydrolases (GH) 11 family and shares a sequence identity of approximately 32.0%-45.0% with fungal xylanases known to trigger plant immune responses. Treatment of Nicotiana benthamiana with purified CcXyn resulted in the induction of hypersensitive response (HR) and defence responses, such as the production of reactive oxygen species (ROS) and upregulation of defence gene expression, ultimately enhancing the resistance of N. benthamiana to Phytophthora nicotianae. These findings indicated that CcXyn functions as a microbe-associated molecular pattern (MAMP) elicitor for plant immune responses, independent of its enzymatic activity. Similar to fungal xylanases, CcXyn was recognized by the NbRXEGL1 receptor on the cell membrane of N. benthamiana. Downstream signalling was shown to be independent of the BAK1 and SOBIR1 co-receptors, indicating the involvement of other co-receptors in signal transduction following CcXyn recognition in N. benthamiana. Moreover, xylanases from other myxobacteria also demonstrated the capacity to trigger plant immune responses in N. benthamiana, indicating that xylanases in myxobacteria are ubiquitous in triggering plant immune functions. This study expands the understanding of xylanases with plant immune response-inducing properties and provides a theoretical basis for potential applications of myxobacteria in biocontrol strategies against phytopathogens.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11196902/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141458069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The F-box protein OsFBX156 positively regulates rice defence against the blast fungus Magnaporthe oryzae by mediating ubiquitination-dependent degradation of OsHSP71.1. F-box 蛋白 OsFBX156 通过介导 OsHSP71.1 的泛素依赖性降解，积极调控水稻对稻瘟病真菌 Magnaporthe oryzae 的防御能力。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-06-01 DOI: 10.1111/mpp.13459

Yudan Zhao, Xionghui Zhong, Guojuan Xu, Xiaoying Zhu, Yanlong Shi, Minghao Liu, Ruyi Wang, Houxiang Kang, Xiaoman You, Yuese Ning, Guo-Liang Wang, Xuli Wang

{"title":"The F-box protein OsFBX156 positively regulates rice defence against the blast fungus Magnaporthe oryzae by mediating ubiquitination-dependent degradation of OsHSP71.1.","authors":"Yudan Zhao, Xionghui Zhong, Guojuan Xu, Xiaoying Zhu, Yanlong Shi, Minghao Liu, Ruyi Wang, Houxiang Kang, Xiaoman You, Yuese Ning, Guo-Liang Wang, Xuli Wang","doi":"10.1111/mpp.13459","DOIUrl":"10.1111/mpp.13459","url":null,"abstract":"F-box protein is a subunit of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which plays a critical role in regulating different pathways in plant immunity. In this study, we identified the rice (Oryza sativa) F-box protein OsFBX156, which targets the heat shock protein 70 (OsHSP71.1) to regulate resistance to the rice blast fungus Magnaporthe oryzae. Overexpression of OsFBX156 or knockout of OsHSP71.1 in rice resulted in the elevation of pathogenesis-related (PR) genes and an induction burst of reactive oxygen species (ROS) after flg22 and chitin treatments, thereby enhancing resistance to M. oryzae. Furthermore, OsFBX156 can promote the degradation of OsHSP71.1 through the 26S proteasome pathway. This study sheds lights on a novel mechanism wherein the F-box protein OsFBX156 targets OsHSP71.1 for degradation to promote ROS production and PR gene expression, thereby positively regulating rice innate immunity.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11134189/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adaptive substitutions at two amino acids of HCPro modify its functional properties to separately increase the virulence of a potyviral chimera. HCPro 两个氨基酸的适应性替代改变了其功能特性，从而分别提高了潜病毒嵌合体的毒力。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-06-01 DOI: 10.1111/mpp.13487

Hao Sun, Malgorzata Ciska, Mongia Makki, Francisco Tenllado, Tomás Canto

{"title":"Adaptive substitutions at two amino acids of HCPro modify its functional properties to separately increase the virulence of a potyviral chimera.","authors":"Hao Sun, Malgorzata Ciska, Mongia Makki, Francisco Tenllado, Tomás Canto","doi":"10.1111/mpp.13487","DOIUrl":"10.1111/mpp.13487","url":null,"abstract":"We had previously reported that a plum pox virus (PPV)-based chimera that had its P1-HCPro bi-cistron replaced by a modified one from potato virus Y (PVY) increased its virulence in some Nicotiana benthamiana plants, after mechanical passages. This correlated with the natural acquisition of amino acid substitutions in several proteins, including in HCPro at either position 352 (Ile→Thr) or 454 (Leu→Arg), or of mutations in non-coding regions. Thr in position 352 is not found among natural potyviruses, while Arg in 454 is a reversion to the native PVY HCPro amino acid. We show here that both mutations separately contributed to the increased virulence observed in the passaged chimeras that acquired them, and that Thr in position 352 is no intragenic suppressor to a Leu in position 454, because their combined effects were cumulative. We demonstrate that Arg in position 454 improved HCPro autocatalytic cleavage, while Thr in position 352 increased its accumulation and the silencing suppression of a reporter in agropatch assays. We assessed infection by four cloned chimera variants expressing HCPro with none of the two substitutions, one of them or both, in wild-type versus DCL2/4-silenced transgenic plants. We found that during infection, the transgenic context of altered small RNAs affected the accumulation of the four HCPro variants differently and hence, also infection virulence.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11178974/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141321246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 4.9 1区农林科学

Molecular plant pathology Pub Date : 2024-06-01 DOI: 10.1111/mpp.13468

Hui Qian, Long Lin, Zhichao Zhang, Xinyi Gu, Danyu Shen, Zhiyuan Yin, Wenwu Ye, Daolong Dou, Yuanchao Wang

{"title":"A MYB-related transcription factor regulates effector gene expression in an oomycete pathogen.","authors":"Hui Qian, Long Lin, Zhichao Zhang, Xinyi Gu, Danyu Shen, Zhiyuan Yin, Wenwu Ye, Daolong Dou, Yuanchao Wang","doi":"10.1111/mpp.13468","DOIUrl":"10.1111/mpp.13468","url":null,"abstract":"Phytophthora pathogens possess hundreds of effector genes that exhibit diverse expression patterns during infection, yet how the expression of effector genes is precisely regulated remains largely elusive. Previous studies have identified a few potential conserved transcription factor binding sites (TFBSs) in the promoters of Phytophthora effector genes. Here, we report a MYB-related protein, PsMyb37, in Phytophthora sojae, the major causal agent of root and stem rot in soybean. Yeast one-hybrid and electrophoretic mobility shift assays showed that PsMyb37 binds to the TACATGTA motif, the most prevalent TFBS in effector gene promoters. The knockout mutant of PsMyb37 exhibited significantly reduced virulence on soybean and was more sensitive to oxidative stress. Consistently, transcriptome analysis showed that numerous effector genes associated with suppressing plant immunity or scavenging reactive oxygen species were down-regulated in the PsMyb37 knockout mutant during infection compared to the wild-type P. sojae. Several promoters of effector genes were confirmed to drive the expression of luciferase in a reporter assay. These results demonstrate that a MYB-related transcription factor contributes to the expression of effector genes in P. sojae.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11134190/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Positive roles of the Ca²⁺ sensors GbCML45 and GbCML50 in improving cotton Verticillium wilt resistance. Ca2+ 传感器 GbCML45 和 GbCML50 在提高棉花轮纹病抗性中的积极作用。

IF 4.9 1区农林科学

Molecular plant pathology Pub Date : 2024-06-01 DOI: 10.1111/mpp.13483

Feifei Yi, Yuzhe Li, Aosong Song, Xinying Shi, Shanci Hu, Shuang Wu, Lili Shao, Zongyan Chu, Kun Xu, Liangliang Li, Lam-Son Phan Tran, Weiqiang Li, Yingfan Cai

{"title":"Positive roles of the Ca2+ sensors GbCML45 and GbCML50 in improving cotton Verticillium wilt resistance.","authors":"Feifei Yi, Yuzhe Li, Aosong Song, Xinying Shi, Shanci Hu, Shuang Wu, Lili Shao, Zongyan Chu, Kun Xu, Liangliang Li, Lam-Son Phan Tran, Weiqiang Li, Yingfan Cai","doi":"10.1111/mpp.13483","DOIUrl":"10.1111/mpp.13483","url":null,"abstract":"As a universal second messenger, cytosolic calcium (Ca2+) functions in multifaceted intracellular processes, including growth, development and responses to biotic/abiotic stresses in plant. The plant-specific Ca2+ sensors, calmodulin and calmodulin-like (CML) proteins, function as members of the second-messenger system to transfer Ca2+ signal into downstream responses. However, the functions of CMLs in the responses of cotton (Gossypium spp.) after Verticillium dahliae infection, which causes the serious vascular disease Verticillium wilt, remain elusive. Here, we discovered that the expression level of GbCML45 was promoted after V. dahliae infection in roots of cotton, suggesting its potential role in Verticillium wilt resistance. We found that knockdown of GbCML45 in cotton plants decreased resistance while overexpression of GbCML45 in Arabidopsis thaliana plants enhanced resistance to V. dahliae infection. Furthermore, there was physiological interaction between GbCML45 and its close homologue GbCML50 by using yeast two-hybrid and bimolecular fluorescence assays, and both proteins enhanced cotton resistance to V. dahliae infection in a Ca2+-dependent way in a knockdown study. Detailed investigations indicated that several defence-related pathways, including salicylic acid, ethylene, reactive oxygen species and nitric oxide signalling pathways, as well as accumulations of lignin and callose, are responsible for GbCML45- and GbCML50-modulated V. dahliae resistance in cotton. These results collectively indicated that GbCML45 and GbCML50 act as positive regulators to improve cotton Verticillium wilt resistance, providing potential targets for exploitation of improved Verticillium wilt-tolerant cotton cultivars by genetic engineering and molecular breeding.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11146148/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141199202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ZmmiR398b negatively regulates maize resistance to sugarcane mosaic virus infection by targeting ZmCSD2/4/9 ZmmiR398b 通过靶向 ZmCSD2/4/9 负向调节玉米对甘蔗花叶病毒感染的抗性

IF 4.9 1区农林科学

Molecular plant pathology Pub Date : 2024-05-02 DOI: 10.1111/mpp.13462

Xinran Gao, Zhichao Du, Kaiqiang Hao, Sijia Zhang, Jian Li, Jinxiu Guo, Zhiping Wang, Shixue Zhao, Lijun Sang, Mengnan An, Zihao Xia, Yuanhua Wu

{"title":"ZmmiR398b negatively regulates maize resistance to sugarcane mosaic virus infection by targeting ZmCSD2/4/9","authors":"Xinran Gao, Zhichao Du, Kaiqiang Hao, Sijia Zhang, Jian Li, Jinxiu Guo, Zhiping Wang, Shixue Zhao, Lijun Sang, Mengnan An, Zihao Xia, Yuanhua Wu","doi":"10.1111/mpp.13462","DOIUrl":"https://doi.org/10.1111/mpp.13462","url":null,"abstract":"MicroRNAs (miRNAs) are widely involved in various biological processes of plants and contribute to plant resistance against various pathogens. In this study, upon sugarcane mosaic virus (SCMV) infection, the accumulation of maize (Zea mays) miR398b (ZmmiR398b) was significantly reduced in resistant inbred line Chang7‐2, while it was increased in susceptible inbred line Mo17. Degradome sequencing analysis coupled with transient co‐expression assays revealed that ZmmiR398b can target Cu/Zn‐superoxidase dismutase2 (ZmCSD2), ZmCSD4, and ZmCSD9 in vivo, of which the expression levels were all upregulated by SCMV infection in Chang7‐2 and Mo17. Moreover, overexpressing ZmmiR398b (OE398b) exhibited increased susceptibility to SCMV infection, probably by increasing reactive oxygen species (ROS) accumulation, which were consistent with ZmCSD2/4/9‐silenced maize plants. By contrast, silencing ZmmiR398b (STTM398b) through short tandem target mimic (STTM) technology enhanced maize resistance to SCMV infection and decreased ROS levels. Interestingly, copper (Cu)‐gradient hydroponic experiments demonstrated that Cu deficiency promoted SCMV infection while Cu sufficiency inhibited SCMV infection by regulating accumulations of ZmmiR398b and ZmCSD2/4/9 in maize. These results revealed that manipulating the ZmmiR398b‐ZmCSD2/4/9‐ROS module provides a prospective strategy for developing SCMV‐tolerant maize varieties.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140842303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a telomere vector‐based approach to overcome limitations caused by lethal phenotypes in the study of essential genes in Magnaporthe oryzae 开发基于端粒载体的方法，以克服在研究Magnaporthe oryzae的重要基因时因致死表型造成的局限性

IF 4.9 1区农林科学

Molecular plant pathology Pub Date : 2024-05-02 DOI: 10.1111/mpp.13460

Louisa Wirtz, Florencia Casanova, Ulrich Schaffrath, Alex Wegner

{"title":"Development of a telomere vector‐based approach to overcome limitations caused by lethal phenotypes in the study of essential genes in Magnaporthe oryzae","authors":"Louisa Wirtz, Florencia Casanova, Ulrich Schaffrath, Alex Wegner","doi":"10.1111/mpp.13460","DOIUrl":"https://doi.org/10.1111/mpp.13460","url":null,"abstract":"Reverse genetic approaches are common tools in genomics for elucidating gene functions, involving techniques such as gene deletion followed by screening for aberrant phenotypes. If the generation of gene deletion mutants fails, the question arises whether the failure stems from technical issues or because the gene of interest (GOI) is essential, meaning that the deletion causes lethality. In this report, we introduce a novel method for assessing gene essentiality using the phytopathogenic ascomycete Magnaporthe oryzae. The method is based on the observation that telomere vectors are lost in transformants during cultivation without selection pressure. We tested the hypothesis that essential genes can be identified in deletion mutants co‐transformed with a telomere vector. The M. oryzae gene MoPKC, described in literature as essential, was chosen as GOI. Using CRISPR/Cas9 technology transformants with deleted GOI were generated and backed up by a telomere vector carrying a copy of the GOI and conferring fenhexamid resistance. Transformants in which the GOI deletion in the genome was not successful lost the telomere vector on media without fenhexamid. In contrast, transformants with confirmed GOI deletion retained the telomere vector even in absence of fenhexamid selection. In the latter case, the maintenance of the telomere indicates that the GOI is essential for the surveillance of the fungi, as it would have been lost otherwise. The method presented here allows to test for essentiality of genes when no mutants can be obtained from gene deletion approaches, thereby expanding the toolbox for studying gene function in ascomycetes.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140842465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An effector SsCVNH promotes the virulence of Sclerotinia sclerotiorum through targeting class III peroxidase AtPRX71 一种效应子 SsCVNH 通过靶向第三类过氧化物酶 AtPRX71 促进硬核菌的毒力

IF 4.9 1区农林科学

Molecular plant pathology Pub Date : 2024-05-02 DOI: 10.1111/mpp.13464

Ming Ma, Liguang Tang, Rui Sun, Xueliang Lyu, Jiatao Xie, Yanping Fu, Bo Li, Tao Chen, Yang Lin, Xiao Yu, Weidong Chen, Daohong Jiang, Jiasen Cheng

{"title":"An effector SsCVNH promotes the virulence of Sclerotinia sclerotiorum through targeting class III peroxidase AtPRX71","authors":"Ming Ma, Liguang Tang, Rui Sun, Xueliang Lyu, Jiatao Xie, Yanping Fu, Bo Li, Tao Chen, Yang Lin, Xiao Yu, Weidong Chen, Daohong Jiang, Jiasen Cheng","doi":"10.1111/mpp.13464","DOIUrl":"https://doi.org/10.1111/mpp.13464","url":null,"abstract":"Many plant pathogens secrete effector proteins into the host plant to suppress host immunity and facilitate pathogen colonization. The necrotrophic pathogen Sclerotinia sclerotiorum causes severe plant diseases and results in enormous economic losses, in which secreted proteins play a crucial role. SsCVNH was previously reported as a secreted protein, and its expression is significantly upregulated at 3 h after inoculation on the host plant. Here, we further demonstrated that deletion of SsCVNH leads to attenuated virulence. Heterologous expression of SsCVNH in Arabidopsis enhanced pathogen infection, inhibited the host PAMP‐triggered immunity (PTI) response and increased plant susceptibility to S. sclerotiorum. SsCVNH interacted with class III peroxidase AtPRX71, a positive regulator of innate immunity against plant pathogens. SsCVNH could also interact with other class III peroxidases, thus reducing peroxidase activity and suppressing plant immunity. Our results reveal a new infection strategy employed by S. sclerotiorum in which the fungus suppresses the function of class III peroxidases, the major component of PTI to promote its own infection.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140842128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0