Molecular plant pathology最新文献_第5页

Effector CLas0185 targets methionine sulphoxide reductase B1 of Citrus sinensis to promote multiplication of 'Candidatus Liberibacter asiaticus' via enhancing enzymatic activity of ascorbate peroxidase 1. 效应器 CLas0185 以中华柑橘的蛋氨酸亚砜还原酶 B1 为靶标，通过增强抗坏血酸过氧化物酶 1 的酶活性来促进 "亚洲自由杆菌 "的繁殖。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-09-01 DOI: 10.1111/mpp.70002

Shushe Zhang, Xuefeng Wang, Tingchang Zhao, Changyong Zhou

{"title":"Effector CLas0185 targets methionine sulphoxide reductase B1 of Citrus sinensis to promote multiplication of 'Candidatus Liberibacter asiaticus' via enhancing enzymatic activity of ascorbate peroxidase 1.","authors":"Shushe Zhang, Xuefeng Wang, Tingchang Zhao, Changyong Zhou","doi":"10.1111/mpp.70002","DOIUrl":"10.1111/mpp.70002","url":null,"abstract":"Citrus huanglongbing (HLB) has been causing enormous damage to the global citrus industry. As the main causal agent, 'Candidatus Liberibacter asiaticus' (CLas) delivers a set of effectors to modulate host responses, while the modes of action adopted remain largely unclear. Here, we demonstrated that CLIBASIA_00185 (CLas0185) could attenuate reactive oxygen species (ROS)-mediated cell death in Nicotiana benthamiana. Transgenic expression of CLas0185 in Citrus sinensis 'Wanjincheng' enhanced plant susceptibility to CLas. We found that methionine sulphoxide reductase B1 (CsMsrB1) was targeted by the effector, and its abundance was elevated in CLas0185-transgenic citrus plants. Their interaction promoted CLas proliferation. We then determined that CsMsrB1 sustained redox state and enzymatic activity of ascorbate peroxidase 1 (CsAPX1) under oxidative stress. The latter reduced H2O2 accumulation and was associated with host susceptibility to CLas infection. Consistently, citrus plants expressing CLas0185 and CsMsrB1 conferred enhanced APX activity and decreased H2O2 content. Taken together, these findings revealed how CLas0185 benefits CLas colonization by targeting CsMsrB1, which facilitated the antioxidant activity and depressed ROS during pathogen infection.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 9","pages":"e70002"},"PeriodicalIF":4.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11365454/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142109589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiple routes to fungicide resistance: Interaction of Cyp51 gene sequences, copy number and expression. 杀菌剂抗性的多种途径：Cyp51 基因序列、拷贝数和表达的相互作用。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-09-01 DOI: 10.1111/mpp.13498

Corinne J Arnold, Emily A Meyers Hahn, Rebecca Whetten, Laetitia Chartrain, Jitender Cheema, James K M Brown, Christina Cowger

{"title":"Multiple routes to fungicide resistance: Interaction of Cyp51 gene sequences, copy number and expression.","authors":"Corinne J Arnold, Emily A Meyers Hahn, Rebecca Whetten, Laetitia Chartrain, Jitender Cheema, James K M Brown, Christina Cowger","doi":"10.1111/mpp.13498","DOIUrl":"10.1111/mpp.13498","url":null,"abstract":"We examined the molecular basis of triazole resistance in Blumeria graminis f. sp. tritici (wheat mildew, Bgt), a model organism among powdery mildews. Four genetic models for responses to triazole fungicides were identified among US and UK isolates, involving multiple genetic mechanisms. Firstly, only two amino acid substitutions in CYP51B lanosterol demethylase, the target of triazoles, were associated with resistance, Y136F and S509T (homologous to Y137F and S524T in the reference fungus Zymoseptoria tritici). As sequence variation did not explain the wide range of resistance, we also investigated Cyp51B copy number and expression, the latter using both reverse transcription-quantitative PCR and RNA-seq. The second model for resistance involved higher copy number and expression in isolates with a resistance allele; thirdly, however, moderate resistance was associated with higher copy number of wild-type Cyp51B in some US isolates. A fourth mechanism was heteroallelism with multiple alleles of Cyp51B. UK isolates, with significantly higher mean resistance than their US counterparts, had higher mean copy number, a high frequency of the S509T substitution, which was absent from the United States, and in the most resistant isolates, heteroallelism involving both sensitivity residues Y136+S509 and resistance residues F136+T509. Some US isolates were heteroallelic for Y136+S509 and F136+S509, but this was not associated with higher resistance. The obligate biotrophy of Bgt may constrain the tertiary structure and thus the sequence of CYP51B, so other variation that increases resistance may have a selective advantage. We describe a process by which heteroallelism may be adaptive when Bgt is intermittently exposed to triazoles.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 9","pages":"e13498"},"PeriodicalIF":4.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415427/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142291397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic and functional analyses on a Botrytis cinerea multidrug-resistant (MDR) strain provides new insights into the potential molecular mechanisms of MDR and fitness. 对一株具有多重耐药性（MDR）的灰葡萄孢菌进行转录组学和功能分析，为了解多重耐药性和适应性的潜在分子机制提供了新的视角。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-09-01 DOI: 10.1111/mpp.70004

Georgios Sofianos, Edoardo Piombo, Mukesh Dubey, Magnus Karlsson, George Karaoglanidis, Georgios Tzelepis

{"title":"Transcriptomic and functional analyses on a Botrytis cinerea multidrug-resistant (MDR) strain provides new insights into the potential molecular mechanisms of MDR and fitness.","authors":"Georgios Sofianos, Edoardo Piombo, Mukesh Dubey, Magnus Karlsson, George Karaoglanidis, Georgios Tzelepis","doi":"10.1111/mpp.70004","DOIUrl":"10.1111/mpp.70004","url":null,"abstract":"Botrytis cinerea is a notorious pathogen causing pre- and post-harvest spoilage in many economically important crops. Excessive application of site-specific fungicides to control the pathogen has led to the selection of strains possessing target site alterations associated with resistance to these fungicides and/or strains overexpressing efflux transporters associated with multidrug resistance (MDR). MDR in B. cinerea has been correlated with the overexpression of atrB and mfsM2, encoding an ATP-binding cassette (ABC) and a major facilitator superfamily (MFS) transporter, respectively. However, it remains unknown whether other transporters may also contribute to the MDR phenotype. In the current study, the transcriptome of a B. cinerea multidrug-resistant (MDR) field strain was analysed upon exposure to the fungicide fludioxonil, and compared to the B05.10 reference strain. The transcriptome of this field strain displayed significant differences as compared to B05.10, including genes involved in sugar membrane transport, toxin production and virulence. Among the induced genes in the field strain, even before exposure to fludioxonil, were several putatively encoding ABC and MFS transmembrane transporters. Overexpression of a highly induced MFS transporter gene in the B05.10 strain led to an increased tolerance to the fungicides fluopyram and boscalid, indicating an involvement in efflux transport of these compounds. Overall, the data from this study give insights towards better understanding the molecular mechanisms involved in MDR and fitness cost, contributing to the development of more efficient control strategies against this pathogen.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 9","pages":"e70004"},"PeriodicalIF":4.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380696/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142154584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cross-family transfer of the Arabidopsis cell-surface immune receptor LORE to tomato confers sensing of 3-hydroxylated fatty acids and enhanced disease resistance. 拟南芥细胞表面免疫受体 LORE 跨家族转移到番茄后，能感知 3- 羟基化脂肪酸并增强抗病性。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-09-01 DOI: 10.1111/mpp.70005

Sabine Eschrig, Parvinderdeep S Kahlon, Carlos Agius, Andrea Holzer, Ralph Hückelhoven, Claus Schwechheimer, Stefanie Ranf

{"title":"Cross-family transfer of the Arabidopsis cell-surface immune receptor LORE to tomato confers sensing of 3-hydroxylated fatty acids and enhanced disease resistance.","authors":"Sabine Eschrig, Parvinderdeep S Kahlon, Carlos Agius, Andrea Holzer, Ralph Hückelhoven, Claus Schwechheimer, Stefanie Ranf","doi":"10.1111/mpp.70005","DOIUrl":"10.1111/mpp.70005","url":null,"abstract":"Plant pathogens pose a high risk of yield losses and threaten food security. Technological and scientific advances have improved our understanding of the molecular processes underlying host-pathogen interactions, which paves the way for new strategies in crop disease management beyond the limits of conventional breeding. Cross-family transfer of immune receptor genes is one such strategy that takes advantage of common plant immune signalling pathways to improve disease resistance in crops. Sensing of microbe- or host damage-associated molecular patterns (MAMPs/DAMPs) by plasma membrane-resident pattern recognition receptors (PRR) activates pattern-triggered immunity (PTI) and restricts the spread of a broad spectrum of pathogens in the host plant. In the model plant Arabidopsis thaliana, the S-domain receptor-like kinase LIPOOLIGOSACCHARIDE-SPECIFIC REDUCED ELICITATION (AtLORE, SD1-29) functions as a PRR, which senses medium-chain-length 3-hydroxylated fatty acids (mc-3-OH-FAs), such as 3-OH-C10:0, and 3-hydroxyalkanoates (HAAs) of microbial origin to activate PTI. In this study, we show that ectopic expression of the Brassicaceae-specific PRR AtLORE in the solanaceous crop species Solanum lycopersicum leads to the gain of 3-OH-C10:0 immune sensing without altering plant development. AtLORE-transgenic tomato shows enhanced resistance against Pseudomonas syringae pv. tomato DC3000 and Alternaria solani NL03003. Applying 3-OH-C10:0 to the soil before infection induces resistance against the oomycete pathogen Phytophthora infestans Pi100 and further enhances resistance to A. solani NL03003. Our study proposes a potential application of AtLORE-transgenic crop plants and mc-3-OH-FAs as resistance-inducing biostimulants in disease management.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 9","pages":"e70005"},"PeriodicalIF":4.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11375736/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

N-acyl homoserine lactone cell-cell diffusible signalling in the Ralstonia solanacearum species complex. Ralstonia solanacearum 物种复合体中的 N-酰基高丝氨酸内酯细胞-细胞扩散信号。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-08-01 DOI: 10.1111/mpp.13467

Peng Li, Cristina Bez, Yong Zhang, Yinyue Deng, Vittorio Venturi

引用次数: 0

A ribonuclease T2 protein FocRnt2 contributes to the virulence of Fusarium oxysporum f. sp. cubense tropical race 4. 核糖核酸酶 T2 蛋白 FocRnt2 对 Fusarium oxysporum f. sp. cubense tropical race 4 的毒力有促进作用。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-08-01 DOI: 10.1111/mpp.13502

Yanqiu He, Pengfei Li, Xiaoshu Zhou, Shaukat Ali, Jie Zhu, Yini Ma, Jieling Li, Nan Zhang, Huaping Li, Yunfeng Li, Yanfang Nie

{"title":"A ribonuclease T2 protein FocRnt2 contributes to the virulence of Fusarium oxysporum f. sp. cubense tropical race 4.","authors":"Yanqiu He, Pengfei Li, Xiaoshu Zhou, Shaukat Ali, Jie Zhu, Yini Ma, Jieling Li, Nan Zhang, Huaping Li, Yunfeng Li, Yanfang Nie","doi":"10.1111/mpp.13502","DOIUrl":"10.1111/mpp.13502","url":null,"abstract":"Banana Fusarium wilt, caused by Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4), is a major disease of banana plants worldwide. Effector proteins play critical roles in banana-Foc TR4 interaction. Our previous studies highlighted a ribonuclease protein belonging to the T2 family (named as FocRnt2) in the Foc TR4 secretome, which was predicted to be an effector. However, its biological function in Foc TR4 infection is still unclear. Herein, we observed significant expression of FocRnt2 during the early stage of fungal infection in planta. A yeast signal sequence trap assay showed that FocRnt2 contained a functional signal peptide for secretion. FocRnt2 possessed ribonuclease activity that could degrade the banana total RNA in vitro. Subcellular localization showed that FocRnt2 was localized in the nucleus and cytoplasm of Nicotiana benthamiana leaves. Transient expression of FocRnt2 suppressed the expression of salicylic acid- and jasmonic acid-signalling marker genes, reactive oxygen species accumulation, and BAX-mediated cell death in N. benthamiana. FocRnt2 deletion limited fungal penetration, reduced fusaric acid biosynthesis in Foc TR4, and attenuated fungal virulence against banana plants, but had little effect on Foc TR4 growth and sensitivity to various stresses. Furthermore, FocRnt2 deletion mutants induced higher expression of the defence-related genes in banana plants. These results suggest that FocRnt2 plays an important role in full virulence of Foc TR4, further improving our understanding of effector-mediated Foc TR4 pathogenesis.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 8","pages":"e13502"},"PeriodicalIF":4.8,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11310096/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to "A novel MAP kinase-interacting protein MoSmi1 regulates development and pathogenicity in Magnaporthe oryzae". 对 "一种新的与 MAP 激酶相互作用的蛋白 MoSmi1 可调控木格鲁氏杆菌的发育和致病性 "的更正。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-08-01 DOI: 10.1111/mpp.13503

引用次数: 0

Evasion of wheat resistance gene Lr15 recognition by the leaf rust fungus is attributed to the coincidence of natural mutations and deletion in AvrLr15 gene. 叶锈病真菌之所以能识别小麦抗性基因 Lr15，是因为 AvrLr15 基因发生了自然突变和缺失。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-07-01 DOI: 10.1111/mpp.13490

Zhongchi Cui, Songsong Shen, Linshuo Meng, Xizhe Sun, Yuqing Jin, Yuanxia Liu, Daqun Liu, Lisong Ma, Haiyan Wang

{"title":"Evasion of wheat resistance gene Lr15 recognition by the leaf rust fungus is attributed to the coincidence of natural mutations and deletion in AvrLr15 gene.","authors":"Zhongchi Cui, Songsong Shen, Linshuo Meng, Xizhe Sun, Yuqing Jin, Yuanxia Liu, Daqun Liu, Lisong Ma, Haiyan Wang","doi":"10.1111/mpp.13490","DOIUrl":"10.1111/mpp.13490","url":null,"abstract":"Employing race-specific resistance genes remains an effective strategy to protect wheat from leaf rust caused by Puccinia triticina (Pt) worldwide, while the newly emerged Pt races, owing to rapid genetic evolution, frequently overcome the immune response delivered by race-specific resistance genes. The molecular mechanisms underlying the newly evolved virulence Pt pathogen remain unknown. Here, we identified an avirulence protein AvrLr15 from Pt that induced Lr15-dependent immune responses. Heterologously produced AvrLr15 triggered pronounced cell death in Lr15-isogenic wheat leaves. AvrLr15 contains a functional signal peptide, localized to the plant nucleus and cytosol and can suppress BAX-induced cell death. Evasion of Lr15-mediated resistance in wheat was associated with a deletion and point mutations of amino acids in AvrLr15 rather than AvrLr15 gene loss in the Lr15-breaking Pt races, implying that AvrLr15 is required for the virulence function of Pt. Our findings identified the first molecular determinant of wheat race-specific immunity and facilitated the identification of the first AVR/R gene pair in the Pt-wheat pathosystem, which will provide a molecular marker to monitor natural Pt populations and guide the deployment of Lr15-resistant wheat cultivars in the field.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 7","pages":"e13490"},"PeriodicalIF":4.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11217590/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141476970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of Monilinia fructicola sporulation and pathogenicity through eucalyptol-mediated targeting of MfCat2 by Streptomyces lincolnensis strain JCP1-7. 林肯链霉菌 JCP1-7 株通过桉叶油醇介导的 MfCat2 靶向作用抑制果核莫尼菌孢子和致病性。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-07-01 DOI: 10.1111/mpp.13484

Shan Chen, Haorong Yang, Meijun Chen, Weina Liu, Shaorui Tian, Rong Mu, Fan Jia, Changyun Liu, Guanhua Ma, Xianchao Sun, Guokang Chen

{"title":"Inhibition of Monilinia fructicola sporulation and pathogenicity through eucalyptol-mediated targeting of MfCat2 by Streptomyces lincolnensis strain JCP1-7.","authors":"Shan Chen, Haorong Yang, Meijun Chen, Weina Liu, Shaorui Tian, Rong Mu, Fan Jia, Changyun Liu, Guanhua Ma, Xianchao Sun, Guokang Chen","doi":"10.1111/mpp.13484","DOIUrl":"10.1111/mpp.13484","url":null,"abstract":"Peach brown rot, attributed to Monilinia fructicola, presents a significant threat to postharvest peach cultivation, causing losses of up to 80%. With an increasing number of countries, spearheaded by the European Union, imposing bans on chemical agents in fruit production, there is a growing interest in mining highly active antibacterial compounds from biological control strains for postharvest disease management. In this study, we highlight the unique ability of Streptomyces lincolnensis strain JCP1-7 to inhibit M. fructicola sporulation, despite its limited antimicrobial efficacy. Through GC-MS analysis, eucalyptol was identified as the key compound. Fumigation of diseased fruits with eucalyptol at a concentration of 0.0335 μg cm-3 demonstrated an in vivo inhibition rate against M. fructicola of 93.13%, completely suppressing spore formation. Transcriptome analysis revealed the impact of eucalyptol on multiple pathogenesis-related pathways, particularly through the inhibition of catalase 2 (Cat2) expression. Experiments with a MfCat2 knockout strain (ΔMfCat2) showed reduced pathogenicity and sensitivity to JCP1-7 and eucalyptol, suggesting MfCat2 as a potential target of JCP1-7 and eucalyptol against M. fructicola. Our findings elucidate that eucalyptol produced by S. lincolnensis JCP1-7 inhibits M. fructicola sporulation by regulating MfCat2, thereby effectively reducing postharvest peach brown rot occurrence. The use of fumigation of eucalyptol offers insights into peach brown rot management on a large scale, thus making a significant contribution to agricultural research.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 7","pages":"e13484"},"PeriodicalIF":4.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11227988/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141555229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A fatty acid elongase complex regulates cell membrane integrity and septin-dependent host infection by the rice blast fungus. 脂肪酸伸长酶复合物调控细胞膜的完整性以及稻瘟病真菌对隔膜依赖性宿主的感染。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-07-01 DOI: 10.1111/mpp.13494

Jia Su, Youpin Xu, Mingliang Lei, Yingying Meng, Siqi Zhang, Hongrui Liu, Caicun Zhu, Jinhua Chen, Tianxin Zhang, Jiawei Liu, Yunxiang Lin, Zhaorui Yan, Weitao Li, Jing Wang, Xuewei Chen, Min He

{"title":"A fatty acid elongase complex regulates cell membrane integrity and septin-dependent host infection by the rice blast fungus.","authors":"Jia Su, Youpin Xu, Mingliang Lei, Yingying Meng, Siqi Zhang, Hongrui Liu, Caicun Zhu, Jinhua Chen, Tianxin Zhang, Jiawei Liu, Yunxiang Lin, Zhaorui Yan, Weitao Li, Jing Wang, Xuewei Chen, Min He","doi":"10.1111/mpp.13494","DOIUrl":"10.1111/mpp.13494","url":null,"abstract":"Very-long-chain fatty acids (VLCFAs) regulate biophysical properties of cell membranes to determine growth and development of eukaryotes, such as the pathogenesis of the rice blast fungus Magnaporthe oryzae. The fatty acid elongase Elo1 regulates pathogenesis of M. oryzae by modulating VLCFA biosynthesis. However, it remains unknown whether and how Elo1 associates with other factors to regulate VLCFA biosynthesis in fungal pathogens. Here, we identified Ifa38, Phs1 and Tsc13 as interacting proteins of Elo1 by proximity labelling in M. oryzae. Elo1 associated with Ifa38, Phs1 and Tsc13 on the endoplasmic reticulum (ER) membrane to control VLCFA biosynthesis. Targeted gene deletion mutants Δifa38, Δphs1 and Δtsc13 were all similarly impaired as Δelo1 in vegetative growth, conidial morphology, stress responses in ER, cell wall and membrane. These deletion mutants also displayed severe damage in cell membrane integrity and failed to organize the septin ring that is essential for penetration peg formation and pathogenicity. Our study demonstrates that M. oryzae employs a fatty acid elongase complex to regulate VLCFAs for maintaining or remodelling cell membrane structure, which is important for septin-mediated host penetration.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 7","pages":"e13494"},"PeriodicalIF":4.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11246601/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141603898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0