Molecular plant pathology最新文献

Hop Stunt Viroid Expression and Host Responses in Arabidopsis thaliana.

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-04-01 DOI: 10.1111/mpp.70080

Xiaxia Tian, Binhui Zhan, Lingzhu He, Changyong Zhou, Yunlong Ma, Shifang Li, Zhixiang Zhang

{"title":"Hop Stunt Viroid Expression and Host Responses in Arabidopsis thaliana.","authors":"Xiaxia Tian, Binhui Zhan, Lingzhu He, Changyong Zhou, Yunlong Ma, Shifang Li, Zhixiang Zhang","doi":"10.1111/mpp.70080","DOIUrl":"10.1111/mpp.70080","url":null,"abstract":"Arabidopsis thaliana serves as an appealing model for viroid research, though prior infection trials have largely failed. Previous studies have shown that mature circular RNAs of certain viroids can be synthesised in A. thaliana via transgenic methods. Here, we confirm this by introducing a transgene encoding the dimeric cDNA of hop stunt viroid (HSVd) genome and explore the potential of HSVd-expressing transgenic A. thaliana in viroid research. Mature HSVd circular genome RNA was detected in transgenic plants but accumulated to relatively low levels. Small RNA (sRNA) sequencing revealed minimal production of HSVd-derived sRNAs, suggesting inefficient replication. This finding highlights the importance of double-stranded replication intermediates as the primary source of viroid sRNAs. Moreover, the low replication efficiency increases the likelihood of identifying viroid-binding host factors involved in early molecular interactions using transgenic A. thaliana. Transcriptome analysis indicated that HSVd expression significantly altered the expression of thousands of A. thaliana genes, with enrichment in metabolic pathways, biosynthesis, plant hormone signalling, plant-pathogen interactions and MAPK signalling pathways. Interestingly, these pathways align with those observed in cucumber systemically infected with HSVd, suggesting that transgenic A. thaliana mimics systemic viroid infections and offers a promising model for studying viroid-host interactions. Thus, despite the challenges of establishing systemic infection, HSVd-expressing transgenic A. thaliana represents a valuable tool for advancing viroid research.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 4","pages":"e70080"},"PeriodicalIF":4.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950631/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcription Activator FgDDT Interacts With FgISW1 to Regulate Fungal Development and Pathogenicity in the Global Pathogen Fusarium graminearum.

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-04-01 DOI: 10.1111/mpp.70076

Xiaozhen Zhao, Yuxin Qiu, Aning Jiang, Yan Huang, Peixue Ma, Bingqin Yuan, Li Chen, Chengqi Zhang

{"title":"Transcription Activator FgDDT Interacts With FgISW1 to Regulate Fungal Development and Pathogenicity in the Global Pathogen Fusarium graminearum.","authors":"Xiaozhen Zhao, Yuxin Qiu, Aning Jiang, Yan Huang, Peixue Ma, Bingqin Yuan, Li Chen, Chengqi Zhang","doi":"10.1111/mpp.70076","DOIUrl":"10.1111/mpp.70076","url":null,"abstract":"Several DNA-binding homeobox and different transcription factor (DDT)-domain proteins form stable remodelling complexes with imitation switch (ISWI) chromatin remodelling factors. ISWI complexes have been reported to be involved in various biological processes in many eukaryotic species. However, in phytopathogenic fungi, the regulatory mechanisms underlying the functions of DDT-domain proteins in ISWI complexes remain unclear. Here, chromatin immunoprecipitation-sequencing (ChIP-seq) assays were used to demonstrate that FgDDT from Fusarium graminearum was enriched within the promoter regions of genes associated with metabolic and MAPK signalling pathways, thereby activating their expression. Moreover, two additional ISWI genes, FgISW1 and FgISW2, were identified and characterised, with subsequent analyses indicating that the ISWI components FgISW1 and FgDDT are essential for fungal development and pathogenicity rather than FgISW2. Further experiments revealed that FgDDT binds to FgISW1 to form an ISWI complex that activates the expression of functional genes in F. graminearum, consequently contributing to its pathogenicity and development. FgDDT was also observed as highly conserved in Fusarium species but exhibits low similarity to homologues in Homo sapiens and Arabidopsis thaliana, suggesting that functional studies of FgDDT are crucial to uncover its unique role within Fusarium. These findings provide a basis for further understanding the molecular mechanisms by which ISWI complexes function in fungi and contribute to their pathogenicity.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 4","pages":"e70076"},"PeriodicalIF":4.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950633/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clade V MLO Genes Are Negative Modulators of Cucumber Defence Response to Meloidogyne incognita.

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-04-01 DOI: 10.1111/mpp.70078

Xiaoxiao Xie, Jian Ling, Shaoyun Dong, Mingjuan Zhai, Junru Lu, Jianlong Zhao, Xueyong Yang, Xin Dong, Yan Li, Richard G F Visser, Yuling Bai, Zhenchuan Mao, Shengping Zhang, Bingyan Xie

{"title":"Clade V MLO Genes Are Negative Modulators of Cucumber Defence Response to Meloidogyne incognita.","authors":"Xiaoxiao Xie, Jian Ling, Shaoyun Dong, Mingjuan Zhai, Junru Lu, Jianlong Zhao, Xueyong Yang, Xin Dong, Yan Li, Richard G F Visser, Yuling Bai, Zhenchuan Mao, Shengping Zhang, Bingyan Xie","doi":"10.1111/mpp.70078","DOIUrl":"10.1111/mpp.70078","url":null,"abstract":"Cucumber production is seriously constrained by Meloidogyne incognita. Because no resistance resources to the pathogen have been reported, disabling susceptibility genes may represent a novel breeding strategy to introduce resistance against this nematode in cucumber. Here, we studied the clade V MLO genes for their involvement in the interaction between cucumber and M. incognita. Our results showed that Arabidopsis clade V MLO mutants were resistant to M. incognita. Cucumber has three clade V MLO genes, CsaMLO1, CsaMLO8 and CsaMLO11, with upregulated expression upon inoculation with M. incognita. Heterologous overexpression of CsaMLO1, CsaMLO8 and CsaMLO11 in Arabidopsis mutants restored susceptibility to varying degrees. Silencing and knockout of individual clade V MLO genes in cucumber reduced susceptibility to M. incognita. The cucumber CRISPR mutants produced similar fruits as the wild type (WT) did. Although the yields of two single mutants (M111 and M112) and two double mutants (M81 M111 and M81 M112) were reduced compared to WT, the yields of M81 and M82 were not decreased. In summary, clade V MLO genes function as susceptibility genes for M. incognita in cucumber. Among them, CsaMLO8 may be the most promising candidate for M. incognita resistance breeding in cucumber.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 4","pages":"e70078"},"PeriodicalIF":4.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950635/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Characterisation of the Peroxidase Gene Family in Botrytis cinerea and the Role of BcPRD7 in Virulence.

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-04-01 DOI: 10.1111/mpp.70079

Shixuan Zhang, Jialin Wang, Bai Li, Jinping Zang, Hongzhe Cao, Jihong Xing, Jingao Dong, Kang Zhang

{"title":"Molecular Characterisation of the Peroxidase Gene Family in Botrytis cinerea and the Role of BcPRD7 in Virulence.","authors":"Shixuan Zhang, Jialin Wang, Bai Li, Jinping Zang, Hongzhe Cao, Jihong Xing, Jingao Dong, Kang Zhang","doi":"10.1111/mpp.70079","DOIUrl":"10.1111/mpp.70079","url":null,"abstract":"Peroxidase activity is essential for the virulence of a number of plant-pathogenic fungi. However, there are few reports of the systematic analysis of peroxidase genes in Botrytis cinerea. We identified all the peroxidase genes of B. cinerea by searching the fungal peroxidase database and found that the expression levels of BcPRD3, BcPRD7, BcPRD8 and BcPRD10 changed significantly during hyphal development and in response to H2O2 stress treatment and infection of Arabidopsis thaliana by B. cinerea. We found that the hyphae of the mutant strains became more slender, the number and size of the infection structures decreased, the number of conidia decreased and the stress response and virulence decreased significantly. These four genes positively regulated the growth, development and pathogenicity of B. cinerea and participated in osmotic and oxidative stress response and cell integrity maintenance. In addition, we also found that BcPRD7 played important roles in oxidase enzyme activity, ion penetration, the synthesis and metabolism of mycotoxins, and determined the interaction between BcPRD7 and BcHEX, the latter being the major protein of the Woronin body. It is speculated that BcPRD7 may regulate the growth, development and pathogenicity of the pathogen by participating in the development of the Woronin body. The function of peroxidase family genes in B. cinerea was systematically analysed in this study, which provides a solid foundation for the subsequent in-depth elucidation of the relevant regulatory mechanisms and is expected to provide new ideas and strategies for the prevention and control of B. cinerea diseases.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 4","pages":"e70079"},"PeriodicalIF":4.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11959156/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Xanthomonas oryzae Orphan Response Regulator EmvR Is Involved in Virulence, Extracellular Polysaccharide Production and Cell Motility.

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-04-01 DOI: 10.1111/mpp.70083

Pei-Dong Ren, Zeng-Feng Ma, Qing-Qing Liu, Xin-Qi Xia, Gui-Ning Zhu, Ji-Liang Tang, Rui-Fang Li, Guang-Tao Lu

{"title":"Xanthomonas oryzae Orphan Response Regulator EmvR Is Involved in Virulence, Extracellular Polysaccharide Production and Cell Motility.","authors":"Pei-Dong Ren, Zeng-Feng Ma, Qing-Qing Liu, Xin-Qi Xia, Gui-Ning Zhu, Ji-Liang Tang, Rui-Fang Li, Guang-Tao Lu","doi":"10.1111/mpp.70083","DOIUrl":"10.1111/mpp.70083","url":null,"abstract":"Bacteria have evolved a large number of two-component signalling systems (TCSs), which are typically composed of a histidine sensor kinase (HK) and a response regulator (RR), to sense environmental changes and modulate subsequent adaptive responses. Here, we describe the involvement of an orphan single-domain RR named EmvR in the virulence, extracellular polysaccharide (EPS) production and cell motilities of the bacterial leaf streak pathogen Xanthomonas oryzae pv. oryzicola (Xoc), which infects rice leaves mainly via stomata and wounds. Deletion of emvR in Xoc reduced virulence when using spraying inoculation but not when using infiltration inoculation. The emvR deletion mutant displayed weakened spreading and enhanced twitching. Additionally, although deletion of emvR did not significantly affect EPS production, overexpression of emvR significantly increased EPS production. Several standard assays revealed that EmvR physically interacts with PilB and represses its ATPase activity. Combining our data with previous findings that PilB provides the energy for type IV pilus (T4P) biogenesis, we conclude that EmvR plays a vital role in modulating Xoc T4P synthesis and in the early stage of Xoc infection through rice stomata. Moreover, our data reveal that EmvR can also interact with the HK of the TCS ColSXOCgx_4036/ColRXOCgx_4037, which positively and negatively affects Xoc spreading and twitching, respectively. We propose a 'one-to-two' TCS working model for the role of ColSXOCgx_4036, ColRXOCgx_4037, and EmvR in modulating Xoc motility.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 4","pages":"e70083"},"PeriodicalIF":4.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11973254/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143795936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The regulation of auxin receptor gene CsAFB2 by csn-miR393a confers resistance against Colletotrichum gloeosporioides in tea plants. csn-miR393a 对辅助素受体基因 CsAFB2 的调控赋予了茶树对球孢子菌的抗性。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-04-01 DOI: 10.1111/mpp.13499

Anburaj Jeyaraj, Shujing Liu, Rui Han, Yuxin Zhao, Tamilselvi Elango, Yuhua Wang, Xuan Chen, Jing Zhuang, Xinghui Li

{"title":"The regulation of auxin receptor gene CsAFB2 by csn-miR393a confers resistance against Colletotrichum gloeosporioides in tea plants.","authors":"Anburaj Jeyaraj, Shujing Liu, Rui Han, Yuxin Zhao, Tamilselvi Elango, Yuhua Wang, Xuan Chen, Jing Zhuang, Xinghui Li","doi":"10.1111/mpp.13499","DOIUrl":"10.1111/mpp.13499","url":null,"abstract":"Anthracnose, a severe disease caused by Colletotrichum, affects diverse crops and leads to significant economic losses through pronounced fruit/leaf lesions. MicroRNAs (miRNAs) play crucial roles in modulating gene expression in response to disease resistance, defence responses and plant immunity. However, the regulatory mechanisms of miRNAs in responses to Colletotrichum gloeosporioides remain unknown in tea plants. Our study revealed that csn-miR393a targets auxin receptor gene CsAFB2 during resistance to C. gloeosporioides in tea plants by comparing the resistant cultivar Zhongcha108 to the susceptible cultivar Longjing43. Through Nicotiana benthamiana leaf co-transformation assays, we demonstrated that csn-miR393a suppresses the expression of CsAFB2, and csn-miR393a target mimic blocks the function of csn-miR393a, leading to increase in the expression of CsAFB2. Repression of transcripts in tea leaves by antisense oligonucleotides demonstrated that csn-miR393a negatively affects the tea plant defence by regulating reactive oxygen species homoeostasis, PR gene expression and catechin accumulation. To further validate the regulatory mechanisms of csn-miR393a, we developed transgenic tea plants overexpressing CsAFB2, resulting in enhanced resistance responses against C. gloeosporioides. Additionally, transgenic N. benthamiana lines overexpressing a csn-miR393a target mimic provided further evidence that csn-miR393a negatively regulates the tea plant defence response against C. gloeosporioides by suppressing CsAFB2. Therefore, manipulating csn-miR393a or its target gene, CsAFB2, has the potential to strengthen the tea plant's resistance against tea anthracnose.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 4","pages":"e13499"},"PeriodicalIF":4.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950636/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Necrotrophic Phytopathogen-Derived GPI-Anchored Protein Functions as an Elicitor to Activate Plant Immunity and Enhance Resistance.

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-04-01 DOI: 10.1111/mpp.70072

Guangxing Miao, Jiatao Xie, Yanping Fu, Bo Li, Tao Chen, Yang Lin, Xiao Yu, Tom Hsiang, Daohong Jiang, Jiasen Cheng

引用次数: 0

Genomics Research on the Road of Studying Biology and Virulence of Cereal Rust Fungi.

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-04-01 DOI: 10.1111/mpp.70082

Haixia Guan, Peng Zhang, Robert F Park, Yi Ding

{"title":"Genomics Research on the Road of Studying Biology and Virulence of Cereal Rust Fungi.","authors":"Haixia Guan, Peng Zhang, Robert F Park, Yi Ding","doi":"10.1111/mpp.70082","DOIUrl":"10.1111/mpp.70082","url":null,"abstract":"Rust fungi are highly destructive pathogens that pose a significant threat to crop production worldwide, especially cereals. Obligate biotrophy and, in many cases, complex life cycles make rust fungi particularly challenging to study. However, recent rapid advances in sequencing technologies and genomic analysis tools have revolutionised rust fungal research. It is anticipated that the increasing availability and ongoing substantial improvements in genome assemblies will propel the field of rust biology into the post-genomic era, instigating a cascade of research endeavours encompassing multi-omics and gene discoveries. This is especially the case for many cereal rust pathogens, for which continental-scale studies of virulence have been conducted over many years and historical collections of viable isolates have been sequenced and assembled. Genomic analysis plays a crucial role in uncovering the underlying causes of the high variability of virulence and the complexity of population dynamics in rust fungi. Here, we provide an overview of progress in rust genomics, discuss the strategies employed in genomic analysis, and elucidate the strides that will drive cereal rust biology into the post-genomic era.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 4","pages":"e70082"},"PeriodicalIF":4.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11968332/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Foliar Pine Pathogens From Different Kingdoms Share Defence-Eliciting Effector Proteins. 来自不同王国的松树叶片病原体共享防御诱导效应蛋白。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-03-01 DOI: 10.1111/mpp.70065

Mariana Tarallo, Carl H Mesarich, Rebecca L McDougal, Rosie E Bradshaw

{"title":"Foliar Pine Pathogens From Different Kingdoms Share Defence-Eliciting Effector Proteins.","authors":"Mariana Tarallo, Carl H Mesarich, Rebecca L McDougal, Rosie E Bradshaw","doi":"10.1111/mpp.70065","DOIUrl":"10.1111/mpp.70065","url":null,"abstract":"Dothistroma needle blight, Cyclaneusma needle blight and red needle cast are devastating foliar pine diseases caused by the fungi Dothistroma septosporum and Cyclaneusma minus and the oomycete Phytophthora pluvialis, respectively. These pathogens colonise the host apoplast, secreting effector proteins to promote infection and disease. If these effectors are recognised by corresponding host resistance proteins, they activate the plant immune system to stop pathogen growth. We aimed to identify and characterise effectors that are common to all three pathogens. Using D. septosporum as a starting point, three candidate effectors (CEs) were investigated: Ds69335 (a CAP protein) and Ds131885, both of which have sequence and structural similarity to CEs of C. minus and P. pluvialis, and Ds74283, which adopts a β-trefoil fold and has structural rather than sequence similarity to CEs from all three pathogens. Notably, of the CEs investigated, Ds74283 and Ds131885, as well as their homologues from C. minus and P. pluvialis, elicited chlorosis or cell death in Nicotiana species, with Ds131885 and its homologues also triggering cell death in Pinus radiata. In line with these observed responses being related to activation of the plant immune system, the chlorosis triggered by Ds131885 and its homologues was compromised in a Nicotiana benthamiana mutant lacking the extracellular immune system co-receptor, SOBIR1. Such cross-kingdom, plant immune system-activating effectors, whether similar in sequence or structure, might ultimately enable the selection or engineering of durable, broad-spectrum resistance against foliar pine pathogens.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 3","pages":"e70065"},"PeriodicalIF":4.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11872807/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role of a C-Terminal Seven-Amino Acid Motif in TbCSV C3 Protein and Its Interaction With NbPOLA2 in Enhancing Viral Replication. TbCSV C3 蛋白中 C 端 7 个氨基酸基团的作用及其与 NbPOLA2 在增强病毒复制中的相互作用

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-03-01 DOI: 10.1111/mpp.70068

Mingjun Li, Puxin Huang, Zhou Jia, Xinyuan Lang, Lyuxin Wang, Miao Sun, Hussein Ghanem, Gentu Wu, Ling Qing

{"title":"The Role of a C-Terminal Seven-Amino Acid Motif in TbCSV C3 Protein and Its Interaction With NbPOLA2 in Enhancing Viral Replication.","authors":"Mingjun Li, Puxin Huang, Zhou Jia, Xinyuan Lang, Lyuxin Wang, Miao Sun, Hussein Ghanem, Gentu Wu, Ling Qing","doi":"10.1111/mpp.70068","DOIUrl":"10.1111/mpp.70068","url":null,"abstract":"The C3 protein of tobacco curly shoot virus (TbCSV), a possible evolutionary intermediate between truly monopartite begomoviruses and those requiring satellite molecules for infectivity, has been identified as a viral replication enhancer (REn). However, the mechanisms underlying this enhancement are largely unknown. In this study, we generated two mutant infectious clones of TbCSV: one with a deletion of the 3' end region of the C3 gene that does not overlap with C2 (TbCSVdC3) and another in which this region was replaced by a phylogenetically unrelated iLOV gene sequence (TbCSVdC3-iLOV). Our findings highlight the crucial role of the 3' end region of C3 for viral DNA accumulation and further demonstrated that overexpression of TbCSV C3 protein in trans complements the functional deficiency of TbCSVdC3. Further analyses revealed the essential role of the C-terminal seven-amino acid motif from residues 123-129 of C3 in replication enhancement. Previous studies suggested that both intra- and intermolecular interactions of C3/AC3 proteins encoded by some other geminiviruses are vital for their capacity to enhance replication. Interestingly, among the tested potential interactors, NbPOLA2, a subunit of DNA polymerase α, was confirmed to interact with C3 in yeast and in planta. Our findings indicated that NbPOLA2 positively regulates TbCSV replication and infection and that the seven-amino acid motif (residues 123-129) in C3 is required for recruiting NbPOLA2 to facilitate TbCSV replication by mediating the viral double-stranded DNA (dsDNA) replication intermediate synthesis. These findings contribute to our understanding of the mechanisms through which the C3 protein enhances TbCSV replication.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 3","pages":"e70068"},"PeriodicalIF":4.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11872800/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0