Molecular plant pathology最新文献

Hop Stunt Viroid Expression and Host Responses in Arabidopsis thaliana.

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-04-01 DOI: 10.1111/mpp.70080

Xiaxia Tian, Binhui Zhan, Lingzhu He, Changyong Zhou, Yunlong Ma, Shifang Li, Zhixiang Zhang

{"title":"Hop Stunt Viroid Expression and Host Responses in Arabidopsis thaliana.","authors":"Xiaxia Tian, Binhui Zhan, Lingzhu He, Changyong Zhou, Yunlong Ma, Shifang Li, Zhixiang Zhang","doi":"10.1111/mpp.70080","DOIUrl":"10.1111/mpp.70080","url":null,"abstract":"Arabidopsis thaliana serves as an appealing model for viroid research, though prior infection trials have largely failed. Previous studies have shown that mature circular RNAs of certain viroids can be synthesised in A. thaliana via transgenic methods. Here, we confirm this by introducing a transgene encoding the dimeric cDNA of hop stunt viroid (HSVd) genome and explore the potential of HSVd-expressing transgenic A. thaliana in viroid research. Mature HSVd circular genome RNA was detected in transgenic plants but accumulated to relatively low levels. Small RNA (sRNA) sequencing revealed minimal production of HSVd-derived sRNAs, suggesting inefficient replication. This finding highlights the importance of double-stranded replication intermediates as the primary source of viroid sRNAs. Moreover, the low replication efficiency increases the likelihood of identifying viroid-binding host factors involved in early molecular interactions using transgenic A. thaliana. Transcriptome analysis indicated that HSVd expression significantly altered the expression of thousands of A. thaliana genes, with enrichment in metabolic pathways, biosynthesis, plant hormone signalling, plant-pathogen interactions and MAPK signalling pathways. Interestingly, these pathways align with those observed in cucumber systemically infected with HSVd, suggesting that transgenic A. thaliana mimics systemic viroid infections and offers a promising model for studying viroid-host interactions. Thus, despite the challenges of establishing systemic infection, HSVd-expressing transgenic A. thaliana represents a valuable tool for advancing viroid research.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 4","pages":"e70080"},"PeriodicalIF":4.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950631/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcription Activator FgDDT Interacts With FgISW1 to Regulate Fungal Development and Pathogenicity in the Global Pathogen Fusarium graminearum.

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-04-01 DOI: 10.1111/mpp.70076

Xiaozhen Zhao, Yuxin Qiu, Aning Jiang, Yan Huang, Peixue Ma, Bingqin Yuan, Li Chen, Chengqi Zhang

{"title":"Transcription Activator FgDDT Interacts With FgISW1 to Regulate Fungal Development and Pathogenicity in the Global Pathogen Fusarium graminearum.","authors":"Xiaozhen Zhao, Yuxin Qiu, Aning Jiang, Yan Huang, Peixue Ma, Bingqin Yuan, Li Chen, Chengqi Zhang","doi":"10.1111/mpp.70076","DOIUrl":"10.1111/mpp.70076","url":null,"abstract":"Several DNA-binding homeobox and different transcription factor (DDT)-domain proteins form stable remodelling complexes with imitation switch (ISWI) chromatin remodelling factors. ISWI complexes have been reported to be involved in various biological processes in many eukaryotic species. However, in phytopathogenic fungi, the regulatory mechanisms underlying the functions of DDT-domain proteins in ISWI complexes remain unclear. Here, chromatin immunoprecipitation-sequencing (ChIP-seq) assays were used to demonstrate that FgDDT from Fusarium graminearum was enriched within the promoter regions of genes associated with metabolic and MAPK signalling pathways, thereby activating their expression. Moreover, two additional ISWI genes, FgISW1 and FgISW2, were identified and characterised, with subsequent analyses indicating that the ISWI components FgISW1 and FgDDT are essential for fungal development and pathogenicity rather than FgISW2. Further experiments revealed that FgDDT binds to FgISW1 to form an ISWI complex that activates the expression of functional genes in F. graminearum, consequently contributing to its pathogenicity and development. FgDDT was also observed as highly conserved in Fusarium species but exhibits low similarity to homologues in Homo sapiens and Arabidopsis thaliana, suggesting that functional studies of FgDDT are crucial to uncover its unique role within Fusarium. These findings provide a basis for further understanding the molecular mechanisms by which ISWI complexes function in fungi and contribute to their pathogenicity.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 4","pages":"e70076"},"PeriodicalIF":4.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950633/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clade V MLO Genes Are Negative Modulators of Cucumber Defence Response to Meloidogyne incognita.

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-04-01 DOI: 10.1111/mpp.70078

Xiaoxiao Xie, Jian Ling, Shaoyun Dong, Mingjuan Zhai, Junru Lu, Jianlong Zhao, Xueyong Yang, Xin Dong, Yan Li, Richard G F Visser, Yuling Bai, Zhenchuan Mao, Shengping Zhang, Bingyan Xie

{"title":"Clade V MLO Genes Are Negative Modulators of Cucumber Defence Response to Meloidogyne incognita.","authors":"Xiaoxiao Xie, Jian Ling, Shaoyun Dong, Mingjuan Zhai, Junru Lu, Jianlong Zhao, Xueyong Yang, Xin Dong, Yan Li, Richard G F Visser, Yuling Bai, Zhenchuan Mao, Shengping Zhang, Bingyan Xie","doi":"10.1111/mpp.70078","DOIUrl":"10.1111/mpp.70078","url":null,"abstract":"Cucumber production is seriously constrained by Meloidogyne incognita. Because no resistance resources to the pathogen have been reported, disabling susceptibility genes may represent a novel breeding strategy to introduce resistance against this nematode in cucumber. Here, we studied the clade V MLO genes for their involvement in the interaction between cucumber and M. incognita. Our results showed that Arabidopsis clade V MLO mutants were resistant to M. incognita. Cucumber has three clade V MLO genes, CsaMLO1, CsaMLO8 and CsaMLO11, with upregulated expression upon inoculation with M. incognita. Heterologous overexpression of CsaMLO1, CsaMLO8 and CsaMLO11 in Arabidopsis mutants restored susceptibility to varying degrees. Silencing and knockout of individual clade V MLO genes in cucumber reduced susceptibility to M. incognita. The cucumber CRISPR mutants produced similar fruits as the wild type (WT) did. Although the yields of two single mutants (M111 and M112) and two double mutants (M81 M111 and M81 M112) were reduced compared to WT, the yields of M81 and M82 were not decreased. In summary, clade V MLO genes function as susceptibility genes for M. incognita in cucumber. Among them, CsaMLO8 may be the most promising candidate for M. incognita resistance breeding in cucumber.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 4","pages":"e70078"},"PeriodicalIF":4.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950635/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Characterisation of the Peroxidase Gene Family in Botrytis cinerea and the Role of BcPRD7 in Virulence.

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-04-01 DOI: 10.1111/mpp.70079

Shixuan Zhang, Jialin Wang, Bai Li, Jinping Zang, Hongzhe Cao, Jihong Xing, Jingao Dong, Kang Zhang

{"title":"Molecular Characterisation of the Peroxidase Gene Family in Botrytis cinerea and the Role of BcPRD7 in Virulence.","authors":"Shixuan Zhang, Jialin Wang, Bai Li, Jinping Zang, Hongzhe Cao, Jihong Xing, Jingao Dong, Kang Zhang","doi":"10.1111/mpp.70079","DOIUrl":"https://doi.org/10.1111/mpp.70079","url":null,"abstract":"Peroxidase activity is essential for the virulence of a number of plant-pathogenic fungi. However, there are few reports of the systematic analysis of peroxidase genes in Botrytis cinerea. We identified all the peroxidase genes of B. cinerea by searching the fungal peroxidase database and found that the expression levels of BcPRD3, BcPRD7, BcPRD8 and BcPRD10 changed significantly during hyphal development and in response to H2O2 stress treatment and infection of Arabidopsis thaliana by B. cinerea. We found that the hyphae of the mutant strains became more slender, the number and size of the infection structures decreased, the number of conidia decreased and the stress response and virulence decreased significantly. These four genes positively regulated the growth, development and pathogenicity of B. cinerea and participated in osmotic and oxidative stress response and cell integrity maintenance. In addition, we also found that BcPRD7 played important roles in oxidase enzyme activity, ion penetration, the synthesis and metabolism of mycotoxins, and determined the interaction between BcPRD7 and BcHEX, the latter being the major protein of the Woronin body. It is speculated that BcPRD7 may regulate the growth, development and pathogenicity of the pathogen by participating in the development of the Woronin body. The function of peroxidase family genes in B. cinerea was systematically analysed in this study, which provides a solid foundation for the subsequent in-depth elucidation of the relevant regulatory mechanisms and is expected to provide new ideas and strategies for the prevention and control of B. cinerea diseases.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 4","pages":"e70079"},"PeriodicalIF":4.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The regulation of auxin receptor gene CsAFB2 by csn-miR393a confers resistance against Colletotrichum gloeosporioides in tea plants. csn-miR393a 对辅助素受体基因 CsAFB2 的调控赋予了茶树对球孢子菌的抗性。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-04-01 DOI: 10.1111/mpp.13499

Anburaj Jeyaraj, Shujing Liu, Rui Han, Yuxin Zhao, Tamilselvi Elango, Yuhua Wang, Xuan Chen, Jing Zhuang, Xinghui Li

{"title":"The regulation of auxin receptor gene CsAFB2 by csn-miR393a confers resistance against Colletotrichum gloeosporioides in tea plants.","authors":"Anburaj Jeyaraj, Shujing Liu, Rui Han, Yuxin Zhao, Tamilselvi Elango, Yuhua Wang, Xuan Chen, Jing Zhuang, Xinghui Li","doi":"10.1111/mpp.13499","DOIUrl":"10.1111/mpp.13499","url":null,"abstract":"Anthracnose, a severe disease caused by Colletotrichum, affects diverse crops and leads to significant economic losses through pronounced fruit/leaf lesions. MicroRNAs (miRNAs) play crucial roles in modulating gene expression in response to disease resistance, defence responses and plant immunity. However, the regulatory mechanisms of miRNAs in responses to Colletotrichum gloeosporioides remain unknown in tea plants. Our study revealed that csn-miR393a targets auxin receptor gene CsAFB2 during resistance to C. gloeosporioides in tea plants by comparing the resistant cultivar Zhongcha108 to the susceptible cultivar Longjing43. Through Nicotiana benthamiana leaf co-transformation assays, we demonstrated that csn-miR393a suppresses the expression of CsAFB2, and csn-miR393a target mimic blocks the function of csn-miR393a, leading to increase in the expression of CsAFB2. Repression of transcripts in tea leaves by antisense oligonucleotides demonstrated that csn-miR393a negatively affects the tea plant defence by regulating reactive oxygen species homoeostasis, PR gene expression and catechin accumulation. To further validate the regulatory mechanisms of csn-miR393a, we developed transgenic tea plants overexpressing CsAFB2, resulting in enhanced resistance responses against C. gloeosporioides. Additionally, transgenic N. benthamiana lines overexpressing a csn-miR393a target mimic provided further evidence that csn-miR393a negatively regulates the tea plant defence response against C. gloeosporioides by suppressing CsAFB2. Therefore, manipulating csn-miR393a or its target gene, CsAFB2, has the potential to strengthen the tea plant's resistance against tea anthracnose.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 4","pages":"e13499"},"PeriodicalIF":4.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950636/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Necrotrophic Phytopathogen-Derived GPI-Anchored Protein Functions as an Elicitor to Activate Plant Immunity and Enhance Resistance.

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-04-01 DOI: 10.1111/mpp.70072

Guangxing Miao, Jiatao Xie, Yanping Fu, Bo Li, Tao Chen, Yang Lin, Xiao Yu, Tom Hsiang, Daohong Jiang, Jiasen Cheng

引用次数: 0

Foliar Pine Pathogens From Different Kingdoms Share Defence-Eliciting Effector Proteins. 来自不同王国的松树叶片病原体共享防御诱导效应蛋白。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-03-01 DOI: 10.1111/mpp.70065

Mariana Tarallo, Carl H Mesarich, Rebecca L McDougal, Rosie E Bradshaw

{"title":"Foliar Pine Pathogens From Different Kingdoms Share Defence-Eliciting Effector Proteins.","authors":"Mariana Tarallo, Carl H Mesarich, Rebecca L McDougal, Rosie E Bradshaw","doi":"10.1111/mpp.70065","DOIUrl":"10.1111/mpp.70065","url":null,"abstract":"Dothistroma needle blight, Cyclaneusma needle blight and red needle cast are devastating foliar pine diseases caused by the fungi Dothistroma septosporum and Cyclaneusma minus and the oomycete Phytophthora pluvialis, respectively. These pathogens colonise the host apoplast, secreting effector proteins to promote infection and disease. If these effectors are recognised by corresponding host resistance proteins, they activate the plant immune system to stop pathogen growth. We aimed to identify and characterise effectors that are common to all three pathogens. Using D. septosporum as a starting point, three candidate effectors (CEs) were investigated: Ds69335 (a CAP protein) and Ds131885, both of which have sequence and structural similarity to CEs of C. minus and P. pluvialis, and Ds74283, which adopts a β-trefoil fold and has structural rather than sequence similarity to CEs from all three pathogens. Notably, of the CEs investigated, Ds74283 and Ds131885, as well as their homologues from C. minus and P. pluvialis, elicited chlorosis or cell death in Nicotiana species, with Ds131885 and its homologues also triggering cell death in Pinus radiata. In line with these observed responses being related to activation of the plant immune system, the chlorosis triggered by Ds131885 and its homologues was compromised in a Nicotiana benthamiana mutant lacking the extracellular immune system co-receptor, SOBIR1. Such cross-kingdom, plant immune system-activating effectors, whether similar in sequence or structure, might ultimately enable the selection or engineering of durable, broad-spectrum resistance against foliar pine pathogens.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 3","pages":"e70065"},"PeriodicalIF":4.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11872807/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role of a C-Terminal Seven-Amino Acid Motif in TbCSV C3 Protein and Its Interaction With NbPOLA2 in Enhancing Viral Replication. TbCSV C3 蛋白中 C 端 7 个氨基酸基团的作用及其与 NbPOLA2 在增强病毒复制中的相互作用

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-03-01 DOI: 10.1111/mpp.70068

Mingjun Li, Puxin Huang, Zhou Jia, Xinyuan Lang, Lyuxin Wang, Miao Sun, Hussein Ghanem, Gentu Wu, Ling Qing

{"title":"The Role of a C-Terminal Seven-Amino Acid Motif in TbCSV C3 Protein and Its Interaction With NbPOLA2 in Enhancing Viral Replication.","authors":"Mingjun Li, Puxin Huang, Zhou Jia, Xinyuan Lang, Lyuxin Wang, Miao Sun, Hussein Ghanem, Gentu Wu, Ling Qing","doi":"10.1111/mpp.70068","DOIUrl":"10.1111/mpp.70068","url":null,"abstract":"The C3 protein of tobacco curly shoot virus (TbCSV), a possible evolutionary intermediate between truly monopartite begomoviruses and those requiring satellite molecules for infectivity, has been identified as a viral replication enhancer (REn). However, the mechanisms underlying this enhancement are largely unknown. In this study, we generated two mutant infectious clones of TbCSV: one with a deletion of the 3' end region of the C3 gene that does not overlap with C2 (TbCSVdC3) and another in which this region was replaced by a phylogenetically unrelated iLOV gene sequence (TbCSVdC3-iLOV). Our findings highlight the crucial role of the 3' end region of C3 for viral DNA accumulation and further demonstrated that overexpression of TbCSV C3 protein in trans complements the functional deficiency of TbCSVdC3. Further analyses revealed the essential role of the C-terminal seven-amino acid motif from residues 123-129 of C3 in replication enhancement. Previous studies suggested that both intra- and intermolecular interactions of C3/AC3 proteins encoded by some other geminiviruses are vital for their capacity to enhance replication. Interestingly, among the tested potential interactors, NbPOLA2, a subunit of DNA polymerase α, was confirmed to interact with C3 in yeast and in planta. Our findings indicated that NbPOLA2 positively regulates TbCSV replication and infection and that the seven-amino acid motif (residues 123-129) in C3 is required for recruiting NbPOLA2 to facilitate TbCSV replication by mediating the viral double-stranded DNA (dsDNA) replication intermediate synthesis. These findings contribute to our understanding of the mechanisms through which the C3 protein enhances TbCSV replication.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 3","pages":"e70068"},"PeriodicalIF":4.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11872800/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selective Degradation of Cucumber Mosaic Virus RNA3 by Nonsense-Mediated Decay Benefits Viral Early Infection.

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-03-01 DOI: 10.1111/mpp.70070

Danqing Zhao, Md Robel Ahmed, Mengjie Tian, Mengjiao Li, Zhouhang Gu, Qiansheng Liao, Zhiyou Du

{"title":"Selective Degradation of Cucumber Mosaic Virus RNA3 by Nonsense-Mediated Decay Benefits Viral Early Infection.","authors":"Danqing Zhao, Md Robel Ahmed, Mengjie Tian, Mengjiao Li, Zhouhang Gu, Qiansheng Liao, Zhiyou Du","doi":"10.1111/mpp.70070","DOIUrl":"10.1111/mpp.70070","url":null,"abstract":"Nonsense-mediated mRNA decay (NMD) is a critical RNA quality control system in eukaryotes, also playing a role in defending against viral infections. However, research has primarily focused on nonsegmented viruses. To investigate the interaction between NMD and segmented RNA viruses, we used cucumber mosaic virus (CMV), which possesses a tripartite, single-stranded, positive-sense RNA genome. Agroinfiltration assays were performed to assess how CMV RNA segments, or their variants, respond to NMD. We found that CMV genomic segments (RNAs 1-3) exhibit distinct responses to NMD. Specifically, RNA3, which serves as the translation template of the movement protein (MP), is selectively degraded by NMD, unlike RNA1 and RNA2, which encode viral replicase components. This degradation is triggered by the coat protein (CP) sequence and can be mitigated by the trans-expression of the 1a replicase or CP. The 1a protein requires its specific interaction with the Box-B motif of RNA3 to avoid NMD. Importantly, compromising NMD reduces CMV infection during the early stages, suggesting that NMD-mediated RNA3 degradation facilitates initial viral replication. This is supported by observations that MP expression in trans negatively regulates viral RNA replication. We propose a model to illustrate the molecular interplay between NMD and CMV, emphasising the implications of genomic segmentation in NMD-virus interactions.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 3","pages":"e70070"},"PeriodicalIF":4.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11890980/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glycoside Hydrolase Family 16 Enzyme RsEG146 From Rhizoctonia solani AG1 IA Induces Cell Death and Triggers Defence Response in Nicotiana tabacum.

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-03-01 DOI: 10.1111/mpp.70075

Chen Chen, Dongyang Jiang, Xi Li, Xue Ji, Rui Yang, Yuwen Chen, Ying Chen, Shimin Zuo, Xijun Chen

{"title":"Glycoside Hydrolase Family 16 Enzyme RsEG146 From Rhizoctonia solani AG1 IA Induces Cell Death and Triggers Defence Response in Nicotiana tabacum.","authors":"Chen Chen, Dongyang Jiang, Xi Li, Xue Ji, Rui Yang, Yuwen Chen, Ying Chen, Shimin Zuo, Xijun Chen","doi":"10.1111/mpp.70075","DOIUrl":"10.1111/mpp.70075","url":null,"abstract":"Rhizoctonia solani AG1 IA is a harmful necrotrophic fungus responsible for various crop diseases, including maize and rice sheath blight, which can lead to significant production losses. However, the pathogenic mechanisms and the roles of effectors in this pathogen remain poorly understood. In this study, we identified a glycoside hydrolase 16 family gene, RsEG146, from R. solani that was upregulated during its infection of Zea mays leaves. When transiently expressed through agroinfiltration, RsEG146 induced cell death in the leaves of tobacco (Nicotiana tabacum 'Samsun'). The predicted signal peptide of RsEG146 was essential for its cell death-inducing activity, while the conserved enzymic active site was not required. The chitin-binding domain was critical for the cell death-inducing activity of RsEG146, with Gly47 identified as the key residue. Substitution of Gly47 with aspartate, glutamate, or proline significantly impaired the cell death-inducing activity of RsEG146. Additionally, transient and heterogeneous expression of RsEG146 enhanced the pathogenicity of Botrytis cinerea on tobacco, and silencing this gene through spray-induced gene silencing (SIGS) reduced the severity of the disease in maize, indicating that RsEG146 functions as an effector. Furthermore, RsEG146 triggered a plant immune response in tobacco. This study demonstrates that RsEG146 is a potential effector and plays a crucial role in the interactions between R. solani AG1 IA and its host.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 3","pages":"e70075"},"PeriodicalIF":4.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11911542/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0