Molecular plant pathology最新文献

The Phytophthora infestans effector Pi05910 suppresses and destabilizes host glycolate oxidase StGOX4 to promote plant susceptibility. Phytophthora infestans效应子Pi05910抑制并破坏宿主乙醇氧化酶StGOX4的稳定性，从而促进植物的易感性。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-11-01 DOI: 10.1111/mpp.70021

Peiling Zhang, Jinyang Li, Xiuhong Gou, Lin Zhu, Yang Yang, Yilin Li, Yingqi Zhang, Liwen Ding, Assiya Ansabayeva, Yuling Meng, Weixing Shan

{"title":"The Phytophthora infestans effector Pi05910 suppresses and destabilizes host glycolate oxidase StGOX4 to promote plant susceptibility.","authors":"Peiling Zhang, Jinyang Li, Xiuhong Gou, Lin Zhu, Yang Yang, Yilin Li, Yingqi Zhang, Liwen Ding, Assiya Ansabayeva, Yuling Meng, Weixing Shan","doi":"10.1111/mpp.70021","DOIUrl":"10.1111/mpp.70021","url":null,"abstract":"Phytophthora infestans is a notorious oomycete pathogen that causes potato late blight. It secretes numerous effector proteins to manipulate host immunity. Understanding mechanisms underlying their host cell manipulation is crucial for developing disease resistance strategies. Here, we report that the conserved RXLR effector Pi05910 of P. infestans is a genotype-specific avirulence elicitor on potato variety Longshu 12 and contributes virulence by suppressing and destabilizing host glycolate oxidase StGOX4. By performing co-immunoprecipitation, yeast-two-hybrid assays, luciferase complementation imaging, bimolecular fluorescence complementation and isothermal titration calorimetry assays, we identified and confirmed potato StGOX4 as a target of Pi05910. Further analysis revealed that StGOX4 and its homologue NbGOX4 are positive immune regulators against P. infestans, as indicated by infection assays on potato and Nicotiana benthamiana overexpressing StGOX4 and TRV-NbGOX4 plants. StGOX4-mediated disease resistance involves enhanced reactive oxygen species accumulation and activated the salicylic acid signalling pathway. Pi05910 binding inhibited enzymatic activity and destabilized StGOX4. Furthermore, mutagenesis analyses indicated that the 25th residue (tyrosine, Y25) of StGOX4 mediates Pi05910 binding and is required for its immune function. Our results revealed that the core RXLR effector of P. infestans Pi05910 suppresses plant immunity by targeting StGOX4, which results in decreased enzymatic activity and protein accumulation, leading to enhanced plant susceptibility.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11530570/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flg22-facilitated PGPR colonization in root tips and control of root rot. Flg22 促进 PGPR 在根尖定殖并控制根腐病。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-11-01 DOI: 10.1111/mpp.70026

Yanan Li, Yafei Li, Yuepeng Wang, Yanqing Yang, Man Qi, Tongfu Su, Rui Li, Dehai Liu, Yuqian Gao, Yuancheng Qi, Liyou Qiu

{"title":"Flg22-facilitated PGPR colonization in root tips and control of root rot.","authors":"Yanan Li, Yafei Li, Yuepeng Wang, Yanqing Yang, Man Qi, Tongfu Su, Rui Li, Dehai Liu, Yuqian Gao, Yuancheng Qi, Liyou Qiu","doi":"10.1111/mpp.70026","DOIUrl":"10.1111/mpp.70026","url":null,"abstract":"Plant root border cells (RBCs) prevent the colonization of plant growth-promoting rhizobacteria (PGPR) at the root tip, rendering the PGPR unable to effectively control pathogens infecting the root tip. In this study, we engineered four strains of Pseudomonas sp. UW4, a typical PGPR strain, each carrying an enhanced green fluorescent protein (EGFP)-expressing plasmid. The UW4E strain harboured only the plasmid, whereas the UW4E-flg22 strain expressed a secreted EGFP-Flg22 fusion protein, the UW4E-Flg(flg22) strain expressed a non-secreted Flg22, and the UW4E-flg22-D strain expressed a secreted Flg22-DNase fusion protein. UW4E-flg22 and UW4E-flg22-D, which secreted Flg22, induced an immune response in wheat RBCs and colonized wheat root tips, whereas the other strains, which did not secrete Flg22, failed to elicit this response and did not colonize wheat root tips. The immune response revealed that wheat RBCs synthesized mucilage, extracellular DNA, and reactive oxygen species. Furthermore, the Flg22-secreting strains showed a 33.8%-93.8% higher colonization of wheat root tips and reduced the root rot incidence caused by Rhizoctonia solani and Fusarium pseudograminearum by 24.6%-35.7% compared to the non-Flg22-secreting strains in pot trials. There was a negative correlation between the incidence of wheat root rot and colonization of wheat root tips by these strains. In contrast, wheat root length and dry weight were positively correlated with the colonization of wheat root tips by these strains. These results demonstrate that engineered secretion of Flg22 by PGPR is an effective strategy for controlling root rot and improving plant growth.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11534644/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142575777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Herbicides as fungicides: Targeting heme biosynthesis in the maize pathogen Ustilago maydis. 作为杀菌剂的除草剂：以玉米病原体 Ustilago maydis 的血红素生物合成为目标。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-11-01 DOI: 10.1111/mpp.70007

Djihane Damoo, Matthias Kretschmer, Christopher W J Lee, Cornelia Herrfurth, Ivo Feussner, Kai Heimel, James W Kronstad

{"title":"Herbicides as fungicides: Targeting heme biosynthesis in the maize pathogen Ustilago maydis.","authors":"Djihane Damoo, Matthias Kretschmer, Christopher W J Lee, Cornelia Herrfurth, Ivo Feussner, Kai Heimel, James W Kronstad","doi":"10.1111/mpp.70007","DOIUrl":"10.1111/mpp.70007","url":null,"abstract":"Pathogens must efficiently acquire nutrients from host tissue to proliferate, and strategies to block pathogen access therefore hold promise for disease control. In this study, we investigated whether heme biosynthesis is an effective target for ablating the virulence of the phytopathogenic fungus Ustilago maydis on maize plants. We first constructed conditional heme auxotrophs of the fungus by placing the heme biosynthesis gene hem12 encoding uroporphyrinogen decarboxylase (Urod) under the control of nitrogen or carbon source-regulated promoters. These strains were heme auxotrophs under non-permissive conditions and unable to cause disease in maize seedlings, thus demonstrating the inability of the fungus to acquire sufficient heme from host tissue to support proliferation. Subsequent experiments characterized the role of endocytosis in heme uptake, the susceptibility of the fungus to heme toxicity as well as the transcriptional response to exogenous heme. The latter RNA-seq experiments identified a candidate ABC transporter with a role in the response to heme and xenobiotics. Given the importance of heme biosynthesis for U. maydis pathogenesis, we tested the ability of the well-characterized herbicide BroadStar to influence disease. This herbicide contains the active ingredient flumioxazin, an inhibitor of Hem14 in the heme biosynthesis pathway, and we found that it was an effective antifungal agent for blocking disease in maize. Thus, repurposing herbicides for which resistant plants are available may be an effective strategy to control pathogens and achieve crop protection.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11530707/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The TOR signalling pathway in fungal phytopathogens: A target for plant disease control. 真菌植物病原体中的 TOR 信号通路：植物病害控制的目标

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-11-01 DOI: 10.1111/mpp.70024

Yun Song, Yaru Wang, Huafang Zhang, Muhammad Abu Bakar Saddique, Xiumei Luo, Maozhi Ren

{"title":"The TOR signalling pathway in fungal phytopathogens: A target for plant disease control.","authors":"Yun Song, Yaru Wang, Huafang Zhang, Muhammad Abu Bakar Saddique, Xiumei Luo, Maozhi Ren","doi":"10.1111/mpp.70024","DOIUrl":"10.1111/mpp.70024","url":null,"abstract":"Plant diseases caused by fungal phytopathogens have led to significant economic losses in agriculture worldwide. The management of fungal diseases is mainly dependent on the application of fungicides, which are not suitable for sustainable agriculture, human health, and environmental safety. Thus, it is necessary to develop novel targets and green strategies to mitigate the losses caused by these pathogens. The target of rapamycin (TOR) complexes and key components of the TOR signalling pathway are evolutionally conserved in pathogens and closely related to the vegetative growth and pathogenicity. As indicated in recent systems, chemical, genetic, and genomic studies on the TOR signalling pathway, phytopathogens with TOR dysfunctions show severe growth defects and nonpathogenicity, which makes the TOR signalling pathway to be developed into an ideal candidate target for controlling plant disease. In this review, we comprehensively discuss the current knowledge on components of the TOR signalling pathway in microorganisms and the diverse roles of various plant TOR in response to plant pathogens. Furthermore, we analyse a range of disease management strategies that rely on the TOR signalling pathway, including genetic modification technologies and chemical controls. In the future, disease control strategies based on the TOR signalling network are expected to become a highly effective weapon for crop protection.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11541241/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142591344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel protein elicitor (Cs08297) from Ciboria shiraiana enhances plant disease resistance. 一种来自 Ciboria shiraiana 的新型蛋白诱导剂（Cs08297）可增强植物的抗病性。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-11-01 DOI: 10.1111/mpp.70023

Shuai Zhang, Ruolan Li, Wei Fan, Xuefei Chen, Caiquan Tao, Shuman Liu, Panpan Zhu, Shuchang Wang, Aichun Zhao

{"title":"A novel protein elicitor (Cs08297) from Ciboria shiraiana enhances plant disease resistance.","authors":"Shuai Zhang, Ruolan Li, Wei Fan, Xuefei Chen, Caiquan Tao, Shuman Liu, Panpan Zhu, Shuchang Wang, Aichun Zhao","doi":"10.1111/mpp.70023","DOIUrl":"10.1111/mpp.70023","url":null,"abstract":"Ciboria shiraiana is a necrotrophic fungus that causes mulberry sclerotinia disease resulting in huge economic losses in agriculture. During infection, the fungus uses immunity elicitors to induce plant tissue necrosis that could facilitate its colonization on plants. However, the key elicitors and immune mechanisms remain unclear in C. shiraiana. Herein, a novel elicitor Cs08297 secreted by C. shiraiana was identified, and it was found to target the apoplast in plants to induce cell death. Cs08297 is a cysteine-rich protein unique to C. shiraiana, and cysteine residues in Cs08297 were crucial for its ability to induce cell death. Cs08297 induced a series of defence responses in Nicotiana benthamiana, including the burst of reactive oxygen species (ROS), callose deposition, and activation of defence-related genes. Cs08297 induced-cell death was mediated by leucine-rich repeat (LRR) receptor-like kinases BAK1 and SOBIR1. Purified His-tagged Cs08297-thioredoxin fusion protein triggered cell death in different plants and enhanced plant resistance to diseases. Cs08297 was necessary for sclerotial development, oxidative-stress adaptation, and cell wall integrity but negatively regulated virulence of C. shiraiana. In conclusion, our results revealed that Cs08297 is a novel fungal elicitor in fungi inducing plant immunity. Furthermore, its potential to enhance plant resistance provides a new target to control agricultural diseases biologically.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11534627/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142575675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A single phosphorylatable amino acid residue is essential for the recognition of multiple potyviral HCPro effectors by potato Ny_tbr. 单个可磷酸化氨基酸残基对于马铃薯 Nytbr 识别多种壶状病毒 HCPro 效应体至关重要。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-11-01 DOI: 10.1111/mpp.70027

Bryce G Alex, Zong-Ying Zhang, Danny Lasky, Hernan Garcia-Ruiz, Ronnie Dewberry, Caitilyn Allen, Dennis Halterman, Aurélie M Rakotondrafara

{"title":"A single phosphorylatable amino acid residue is essential for the recognition of multiple potyviral HCPro effectors by potato Nytbr.","authors":"Bryce G Alex, Zong-Ying Zhang, Danny Lasky, Hernan Garcia-Ruiz, Ronnie Dewberry, Caitilyn Allen, Dennis Halterman, Aurélie M Rakotondrafara","doi":"10.1111/mpp.70027","DOIUrl":"10.1111/mpp.70027","url":null,"abstract":"Potato virus Y (PVY, Potyviridae) is among the most important viral pathogens of potato. The potato resistance gene Nytbr confers hypersensitive resistance to the ordinary strain of PVY (PVYO), but not the necrotic strain (PVYN). Here, we unveil that residue 247 of PVY helper component proteinase (HCPro) acts as a central player controlling Nytbr strain-specific activation. We found that substituting the serine at 247 in the HCPro of PVYO (HCProO) with an alanine as in PVYN HCPro (HCProN) disrupts Nytbr recognition. Conversely, an HCProN mutant carrying a serine at position 247 triggers defence. Moreover, we demonstrate that plant defences are induced against HCProO mutants with a phosphomimetic or another phosphorylatable residue at 247, but not with a phosphoablative residue, suggesting that phosphorylation could modulate Nytbr resistance. Extending beyond PVY, we establish that the same response elicited by the PVYO HCPro is also induced by HCPro proteins from other members of the Potyviridae family that have a serine at position 247, but not by those with an alanine. Together, our results provide further insights in the strain-specific PVY resistance in potato and infer a broad-spectrum detection mechanism of plant potyvirus effectors contingent on a single amino acid residue.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11541239/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142591341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing RNA interference for the control of Fusarium species: A critical review. 利用 RNA 干扰来控制镰刀菌：重要综述。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-10-01 DOI: 10.1111/mpp.70011

Caihong Liu, Karl-Heinz Kogel, Maria Ladera-Carmona

{"title":"Harnessing RNA interference for the control of Fusarium species: A critical review.","authors":"Caihong Liu, Karl-Heinz Kogel, Maria Ladera-Carmona","doi":"10.1111/mpp.70011","DOIUrl":"10.1111/mpp.70011","url":null,"abstract":"Fusarium fungi are a pervasive threat to global agricultural productivity. They cause a spectrum of plant diseases that result in significant yield losses and threaten food safety by producing mycotoxins that are harmful to human and animal health. In recent years, the exploitation of the RNA interference (RNAi) mechanism has emerged as a promising avenue for the control of Fusarium-induced diseases, providing both a mechanistic understanding of Fusarium gene function and a potential strategy for environmentally sustainable disease management. However, despite significant progress in elucidating the presence and function of the RNAi pathway in different Fusarium species, a comprehensive understanding of its individual protein components and underlying silencing mechanisms remains elusive. Accordingly, while a considerable number of RNAi-based approaches to Fusarium control have been developed and many reports of RNAi applications in Fusarium control under laboratory conditions have been published, the applicability of this knowledge in agronomic settings remains an open question, and few convincing data on RNAi-based disease control under field conditions have been published. This review aims to consolidate the current knowledge on the role of RNAi in Fusarium disease control by evaluating current research and highlighting important avenues for future investigation.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11450251/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142372250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New persistent plant RNA virus carries mutations to weaken viral suppression of antiviral RNA interference. 新型持久性植物 RNA 病毒携带突变，削弱了病毒对抗病毒 RNA 干扰的抑制作用。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-10-01 DOI: 10.1111/mpp.70020

Li-Juan Zhu, Yu Zhu, Chengwu Zou, Lan-Yi Su, Chong-Tao Zhang, Chi Wang, Ya-Ni Bai, Baoshan Chen, Rongbai Li, Qingfa Wu, Shou-Wei Ding, Jian-Guo Wu, Yan-Hong Han

{"title":"New persistent plant RNA virus carries mutations to weaken viral suppression of antiviral RNA interference.","authors":"Li-Juan Zhu, Yu Zhu, Chengwu Zou, Lan-Yi Su, Chong-Tao Zhang, Chi Wang, Ya-Ni Bai, Baoshan Chen, Rongbai Li, Qingfa Wu, Shou-Wei Ding, Jian-Guo Wu, Yan-Hong Han","doi":"10.1111/mpp.70020","DOIUrl":"10.1111/mpp.70020","url":null,"abstract":"Persistent plant viruses are widespread in natural ecosystems. However, little is known about why persistent infection with these viruses may cause little or no harm to their host. Here, we discovered a new polerovirus that persistently infected wild rice plants by deep sequencing and assembly of virus-derived small-interfering RNAs (siRNAs). The new virus was named Rice tiller inhibition virus 2 (RTIV2) based on the symptoms developed in cultivated rice varieties following Agrobacterium-mediated inoculation with an infectious RTIV2 clone. We showed that RTIV2 infection induced antiviral RNA interference (RNAi) in both the wild and cultivated rice plants as well as Nicotiana benthamiana. It is known that virulent virus infection in plants depends on effective suppression of antiviral RNAi by viral suppressors of RNAi (VSRs). Notably, the P0 protein of RTIV2 exhibited weak VSR activity and carries alanine substitutions of two amino acids broadly conserved among diverse poleroviruses. Mixed infection with umbraviruses enhanced RTIV2 accumulation and/or enabled its mechanical transmission in N. benthamiana. Moreover, replacing the alanine at either one or both positions of RTIV2 P0 enhanced the VSR activity in a co-infiltration assay, and RTIV2 mutants carrying the corresponding substitutions replicated to significantly higher levels in both rice and N. benthamiana plants. Together, our findings show that as a persistent plant virus, RTIV2 carries specific mutations in its VSR gene to weaken viral suppression of antiviral RNAi. Our work reveals a new strategy for persistent viruses to maintain long-term infection by weak suppression of the host defence response.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11513406/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142504361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autophagy plays an antiviral defence role against tomato spotted wilt orthotospovirus and is counteracted by viral effector NSs. 自噬对番茄斑点萎蔫病正交病毒起着抗病毒防御作用，并被病毒效应子 NSs 抵消。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-10-01 DOI: 10.1111/mpp.70012

Xingwang Zhang, Hao Hong, Jiaoling Yan, Yulong Yuan, Mingfeng Feng, Qinhai Liu, Yanxiao Zhao, Tongqing Yang, Shen Huang, Chunli Wang, Ruizhen Zhao, Wenyu Zuo, Suyu Liu, Zixuan Ding, Changjun Huang, Zhongkai Zhang, Jiban Kumar Kundu, Xiaorong Tao

{"title":"Autophagy plays an antiviral defence role against tomato spotted wilt orthotospovirus and is counteracted by viral effector NSs.","authors":"Xingwang Zhang, Hao Hong, Jiaoling Yan, Yulong Yuan, Mingfeng Feng, Qinhai Liu, Yanxiao Zhao, Tongqing Yang, Shen Huang, Chunli Wang, Ruizhen Zhao, Wenyu Zuo, Suyu Liu, Zixuan Ding, Changjun Huang, Zhongkai Zhang, Jiban Kumar Kundu, Xiaorong Tao","doi":"10.1111/mpp.70012","DOIUrl":"10.1111/mpp.70012","url":null,"abstract":"Autophagy, an intracellular degradation process, has emerged as a crucial innate immune response against various plant pathogens, including viruses. Tomato spotted wilt orthotospovirus (TSWV) is a highly destructive plant pathogen that infects over 1000 plant species and poses a significant threat to global food security. However, the role of autophagy in defence against the TSWV pathogen, and whether the virus counteracts this defence, remains unknown. In this study, we report that autophagy plays an important role in antiviral defence against TSWV infection; however, this autophagy-mediated defence is counteracted by the viral effector NSs. Transcriptome profiling revealed the up-regulation of autophagy-related genes (ATGs) upon TSWV infection. Blocking autophagy induction by chemical treatment or knockout/down of ATG5/ATG7 significantly enhanced TSWV accumulation. Notably, the TSWV nucleocapsid (N) protein, a major component of the viral replication unit, strongly induced autophagy. However, the TSWV nonstructural protein NSs was able to effectively suppress N-induced autophagy in a dose-dependent manner. Further investigation revealed that NSs inhibited ATG6-mediated autophagy induction. These findings provide new insights into the defence role of autophagy against TSWV, a representative segmented negative-strand RNA virus, as well as the tospoviral pathogen counterdefence mechanism.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11442783/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142350330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editing of the MeSWEET10a promoter yields bacterial blight resistance in cassava cultivar SC8. 编辑 MeSWEET10a 启动子可使木薯栽培品种 SC8 获得细菌性枯萎病抗性。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-10-01 DOI: 10.1111/mpp.70010

Yajie Wang, Mengting Geng, Ranran Pan, Tong Zhang, Xiaohua Lu, Xinghou Zhen, Yannian Che, Ruimei Li, Jiao Liu, Yinhua Chen, Jianchun Guo, Yuan Yao

{"title":"Editing of the MeSWEET10a promoter yields bacterial blight resistance in cassava cultivar SC8.","authors":"Yajie Wang, Mengting Geng, Ranran Pan, Tong Zhang, Xiaohua Lu, Xinghou Zhen, Yannian Che, Ruimei Li, Jiao Liu, Yinhua Chen, Jianchun Guo, Yuan Yao","doi":"10.1111/mpp.70010","DOIUrl":"10.1111/mpp.70010","url":null,"abstract":"Cassava starch is a widely used raw material for industrial production and food source for people. However, cassava bacterial blight (CBB) caused by Xanthomonas axonopodis pv. manihotis (Xam) results in severe yield losses and is the most destructive bacterial disease in all worldwide cassava-growing regions. Xam11 is a highly pathogenic subspecies from China that infects the Chinese local cassava South China No. 8 (SC8) cultivar with marked symptoms. This study showed that the transcription activator-like effector TALE20Xam11 of Xam11 strain regulates the expression of disease-susceptibility gene MeSWEET10a by binding to the EBETALE20 region of the MeSWEET10a promoter in cassava cultivar SC8. CRISPR/Cas9-generated mutations of the EBETALE20 region resulted in a significant reduction in MeSWEET10a expression after infection by Xam11, correlating with reduced disease symptoms, smaller lesion sizes and decreased bacterial proliferation compared with the wild type. Importantly, the edited plants maintained normal growth, development and yield characteristics under greenhouse conditions. The results lay a research foundation for breeding resistant cassava cultivar SC8 to bacterial blight.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11439743/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142350331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0