Molecular plant pathology最新文献

{"title":"Mutations in the SWEET15 Sugar Transporter Gene Affect Response of Citrus to Huanglongbing Disease and Citrus Canker.","authors":"Archana Khadgi, Omar Zayed, Cintia H D Sagawa, Fei Zhang, Danelle K Seymour, Vivian F Irish","doi":"10.1111/mpp.70094","DOIUrl":"10.1111/mpp.70094","url":null,"abstract":"<p><p>Bacterial diseases like huanglongbing (HLB) and citrus canker severely impact citrus production. HLB, caused by \"Candidatus Liberibacter asiaticus\" (CLas), leads to tree decline, while citrus canker, caused by Xanthomonas citri pv. citri (Xcc) causes necrotic lesions on leaves and fruit. Many bacterial pathogens secrete effector proteins that suppress host plant immunity and promote pathogenesis through the upregulation of host-encoded susceptibility genes. Xcc uses the type III secretion system to introduce effector proteins such as the transcription factor-like (TAL) effector PthA4 that can directly activate host susceptibility gene expression. In contrast, CLas lacks most bacterial secretion systems and relies predominantly on the Sec secretion system for pathogenesis. While some Sec-secreted proteins have been identified in CLas, their direct role in causing HLB symptoms remains unproven. Several Sugars Will Eventually be Exported Transporter (SWEET) genes, encoding sucrose transporters, are candidate susceptibility genes. Here we investigate the roles of the citrus SWEET10, SWEET12 and SWEET15 genes and show that mutations of SWEET15 resulted in reduced susceptibility to citrus canker in three different citrus cultivars: Carrizo citrange (Citrus sinensis 'Washington' sweet orange × Poncirus trifoliata), 'Limoneria 8A' Lisbon lemon (Citrus limon) and 'Pineapple' sweet orange (C. sinensis). Furthermore, Lisbon lemon plants mutated for SWEET15 also showed reduced CLas titre in infected plants. These results suggest that SWEET15 may act as a broad-spectrum susceptibility gene, and disruption of SWEET15 gene activity could be a viable approach to mitigating bacterial diseases such as citrus canker and HLB in a variety of citrus cultivars.</p>","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 5","pages":"e70094"},"PeriodicalIF":4.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12078760/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144079129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Betulinic Acid Delays Turnip Mosaic Virus Infection by Activating the Phytosulfokine Signalling Pathway in Nicotiana benthamiana.","authors":"Meirong Xiang, Pengyue Wang, Keda Han, Jianjian Liu, Ziting Huang, Chaonan Wang, Xinxin Jing, Jiao Du, Bingjian Sun, Honglian Li, Chao Zhang, Pengbai Li","doi":"10.1111/mpp.70092","DOIUrl":"https://doi.org/10.1111/mpp.70092","url":null,"abstract":"<p><p>Plant viral diseases pose a significant threat to agricultural production, and the availability of effective drugs against viral diseases remains limited. In this study, we discovered that betulinic acid (BA), a pentacyclic triterpenoid derived from plants, delays infection by turnip mosaic virus (TuMV) in Nicotiana benthamiana. Transcriptomic analysis revealed that BA treatment specifically induced the expression of N. benthamiana phytosulfokine 3 (NbPSK3), a plant pentapeptide hormone with diverse functions, while TuMV infection suppressed its expression. Further study demonstrated that NbPSK3 positively regulates antiviral defence against TuMV infection. Disruption of PSK signalling by targeting the membrane-bound PSK receptors (PSKRs) promoted viral infection. Additionally, exogenous sulphonated PSK (active form) treatment significantly delayed infection by TuMV in N. benthamiana compared to unmodified PSK peptides (dPSK, inactive form) or control treatments, while silencing the receptor NbPSKR1 abolished the ability of PSK to inhibit TuMV infection. Moreover, the inhibition of TuMV infection by BA is dependent on the PSK-PSKR signalling pathway. Overall, these findings not only underscore the potential of BA as a promising and environmentally friendly agent for modulating plant viral diseases but also emphasise the role of the PSK signalling pathway in promoting at least partial resistance to TuMV, which might have interest for crop breeding.</p>","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 5","pages":"e70092"},"PeriodicalIF":4.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12066822/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144033971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting of the Hybrid Bamboo BDDnaJ by Pathogen Effector ApcE12 Regulates the Unfolded Protein Response.","authors":"Fengying Luo, Xinmei Fang, Haiyan Luo, Ziqi Ye, Sijia Liu, Han Zhao, Shuying Li, Shujiang Li","doi":"10.1111/mpp.70089","DOIUrl":"https://doi.org/10.1111/mpp.70089","url":null,"abstract":"<p><p>The shoot blight disease of Bambusa pervariabilis × Dendrocalamopsis grandis, caused by Arthrinium phaeospermum, threatens bamboo's ecological and economic value. This study explores the pathogenic effector ApcE12's role in modulating plant immunity through interactions with the host proteins BDClp and BDDnaJ. ApcE12 directly interacts with BDDnaJ, a vital regulator of the unfolded protein response (UPR), as validated through yeast two-hybrid, bimolecular fluorescence complementation, and GST pull-down assays. Functional analyses demonstrated that silencing BDDnaJ reduces UPR, activating programmed cell death (PCD) and blocking further pathogen infection to enhance plant resistance. BDDnaJ was found to regulate BDBiP protein stability by interacting with BDBiP, and it is this mechanism by which the pathogenic effector ApcE12 regulates BDDnaJ expression, enhances UPR signalling, and inhibits PCD, thereby promoting infection. These findings deepen our understanding of how fungal effectors manipulate UPR and PCD to overcome plant defences, providing novel insights for developing resistance strategies in bamboo species.</p>","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 5","pages":"e70089"},"PeriodicalIF":4.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12040440/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144023906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasmopara viticola Effector PvRXLR10 Targets a Host Phospholipase VvipPLA-IIδ2 to Suppress Plant Immunity in Grapevine.","authors":"Junjie Qu, Lulu Liu, Chengxu Zheng, Zexi Guo, Dayun Sun, Fengying Pan, Jiang Lu, Ling Yin","doi":"10.1111/mpp.70095","DOIUrl":"10.1111/mpp.70095","url":null,"abstract":"<p><p>Plasmopara viticola that causes grapevine downy mildew disease in viticulture regions is among the 10 most relevant pathogens worldwide. It secretes a large arsenal of effectors to facilitate colonisation by perturbing host immunity. However, the underlying mechanisms by which P. viticola effectors disturb grapevine defence are still largely unknown. In this study, we report that PvRXLR10, an RXLR effector with a WY domain, promotes P. viticola infection in grapevine and Phytophthora parasitica colonisation in Nicotiana benthamiana. PvRXLR10 interacts with a host patatin-like protein VvipPLA-IIδ2 with phospholipase A2 activity. The WY domain of PvRXLR10 is not responsible for cell death suppression in N. benthamiana but is necessary for PvRXLR10 interaction with VvipPLA-IIδ2. Overexpression and RNAi-mediated suppression of VvipPLA-IIδ2 expression in Vitis vinifera consistently showed that this protein positively regulates plant immunity in response to P. viticola infection. Interestingly, we found that VvipPLA-IIδ2 partially associates with PvRXLR10 at the endoplasmic reticulum (ER). Reverse transcription-quantitative PCR (RT-qPCR) analysis showed that the expression of VvipPLA-IIδ2 was suppressed by PvRXLR10 during P. viticola infection. The overexpression of VvipPLA-IIδ2 in V. vinifera induced higher expression of genes related to jasmonic acid (JA) biosynthesis, signalling pathways and defence response. The evidence indicates the important roles of VvipPLA-IIδ2 in grapevine immunity and P. viticola effector PvRXLR10 targets this protein to promote its infection.</p>","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 5","pages":"e70095"},"PeriodicalIF":4.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12081833/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144079064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Valsa mali Effector VmSR1 Accelerates the Degradation of Tudor-SN2 to Suppress RNA Silencing and Plant Immunity.","authors":"Tao Jiang, Chengli Wang, Mian Zhang, Yongli Qiao, Zhensheng Kang, Lili Huang","doi":"10.1111/mpp.70097","DOIUrl":"https://doi.org/10.1111/mpp.70097","url":null,"abstract":"<p><p>The apple Valsa canker caused by Valsa mali is one of the most destructive trunk diseases in apple production and disease management. Understanding the interaction between the pathogen and host is a critical foundation for developing durable disease control technologies. In this study, we showed that VmSR1 from V. mali can suppress the plant immune response and promote pathogen infection. VmSR1 associates with the Tudor staphylococcal nuclease 2 (TSN2) proteins in Malus domestica (apple), Arabidopsis thaliana and Nicotiana benthamiana, promotes degradation of TSN2 proteins, and suppresses the abundance of multiple miRNAs an Silencing of TSN2 significantly reduced the abundance of miRNAs and weakened the resistance of apple leaves to V. mali as well as N. benthamiana to Sclerotinia sclerotiorum. These findings expand the understanding of the function of effectors as RNA silencing suppressors during host-pathogen interactions and deepen the understanding of effectors regulating host immunity.</p>","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 5","pages":"e70097"},"PeriodicalIF":4.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pseudomonas syringae pv. tabaci 6605 Requires Seven Type III Effectors to Infect Nicotiana benthamiana.","authors":"Kana Kuroe, Takafumi Nishimura, Sachi Kashihara, Nanami Sakata, Mikihiro Yamamoto, Yoshiteru Noutoshi, Kazuhiro Toyoda, Yuki Ichinose, Hidenori Matsui","doi":"10.1111/mpp.70091","DOIUrl":"https://doi.org/10.1111/mpp.70091","url":null,"abstract":"<p><p>Type III effectors (T3Es), virulence factors injected into plant cells via the type III secretion system (T3SS), play essential roles in the infection of host plants. Pseudomonas syringae pv. tabaci 6605 (Pta 6605) is the causal agent of wildfire disease in tobacco and harbours at least 22 T3Es in its genome. However, the specific T3Es required by Pta 6605 to infect Nicotiana benthamiana remain unidentified. In this study, we investigated the T3Es that contribute to Pta 6605 infection of N. benthamiana. We constructed Pta 6605 poly-T3E-deficient mutants (Pta DxE) and inoculated them into N. benthamiana. Flood assay, which mimics natural opening-based entry, showed that mutant strains lacking 14-22 T3Es, namely, Pta D14E-D22E mutants, exhibited reduced disease symptoms. By contrast, infiltration inoculation, which involves direct injection into leaves, showed that the Pta D14E to Pta D20E mutants developed disease symptoms. Notably, the Pta D20E, containing AvrE1 and HopM1, induced weak but observable symptoms upon infiltration inoculation. Conversely, no symptoms were observed in either the flood assay or infiltration inoculation for Pta D21E and Pta D22E. Taken together, these findings indicate that the many T3Es such as AvrPto4/AvrPtoB, HopW1/HopAE1, and HopM1/AvrE1 in Pta 6605 collectively contribute to invasion through natural openings and symptom development in N. benthamiana. This study provides the basis for understanding virulence in the host by identifying the minimum T3E repertoire required by Pta 6605 to infect N. benthamiana.</p>","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 5","pages":"e70091"},"PeriodicalIF":4.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12059303/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144024966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic Islands of Pseudomonas syringae pv. tabaci 6605: Identification of PtaGI-1 as a Pathogenicity Island With Effector Genes and a Tabtoxin Cluster.","authors":"Yuta Watanabe, Kotomi Kunishi, Hidenori Matsui, Nanami Sakata, Yoshiteru Noutoshi, Kazuhiro Toyoda, Yuki Ichinose","doi":"10.1111/mpp.70087","DOIUrl":"10.1111/mpp.70087","url":null,"abstract":"<p><p>Genomic islands (GIs) are 20-500 kb DNA regions that are thought to be acquired by horizontal gene transfer. GIs that confer pathogenicity and environmental adaptation have been reported in Pseudomonas species; however, GIs that enhance bacterial virulence have not. Here, we identified 110 kb and 103 kb GIs in P. syringae pv. tabaci 6605 (Pta6605), the causative agent of tobacco wildfire disease, which has the ability to produce tabtoxin as a phytotoxin. These GIs are partially homologous to known genomic islands in Pseudomonas aeruginosa and P. syringae pv. phaseolicola and were designated PtaGI-1 and PtaGI-2. Both PtaGIs conserve core genes, whereas each GI possesses different accessory genes. PtaGI-1 contains a tabtoxin biosynthetic gene cluster and three type III effector genes among its accessory genes, whereas PtaGI-2 also contains homologous genes to hsvABC, pathogenicity-related genes in Erwinia amylovora. Inoculation revealed that the PtaGI-1 mutant, but not the PtaGI-2 mutant, lost the ability to biosynthesise tabtoxin and to cause disease. Therefore, PtaGI-1 is thought to be a pathogenicity island. Both PtaGI-1 and PtaGI-2 have a pseudogene of tRNA^Lys on the left border and an intact tRNA^Lys gene on the right border. In a colony of Pta6605, both GIs can be excised at tRNA^Lys, and PtaGI-1 and PtaGI-2 exist in a circular form. These results indicate that tabtoxin biosynthesis genes in PtaGI-1 are required for disease development, and PtaGI-1 is necessary for Pta6605 virulence.</p>","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 5","pages":"e70087"},"PeriodicalIF":4.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12069800/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144031887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Harpin-Induced Hypersensitive Reaction in Nicotiana tabacum Requires Wall-Associated Kinase 2.","authors":"Jeremy B Held, Judith P Sinn, Brian H Kvitko, Timothy W McNellis","doi":"10.1111/mpp.70096","DOIUrl":"10.1111/mpp.70096","url":null,"abstract":"<p><p>Harpins are proteins secreted by many gram-negative, plant-pathogenic bacteria that stimulate the hypersensitive reaction (HR), a host cell death defence response, when infiltrated into plant leaves as purified proteins. This activity of harpins was first discovered in Nicotiana tabacum (tobacco), which manifests an especially strong and rapid harpin-activated HR that becomes evident within 12-24 h after infiltration. HrpN is the major harpin of the fire blight pathogen Erwinia amylovora. We discovered natural variation in the HrpN-induced HR among tobacco accessions and identified candidate genes using genetic mapping and bulked-segregant analysis with whole genome sequencing. Virus-induced gene silencing of candidate gene Wall-Associated Kinase 2 (WAK2) abrogated the HR in response to HrpN and HpaG, a harpin from the soybean bacterial pustule pathogen Xanthomonas citri pv. glycines. WAK2 silencing also compromised the avirulence activity of harpin HrpZ in the tobacco wildfire pathogen Pseudomonas syringae pv. tabaci. A natural, disruptive mutation in WAK2 correlated with the inability of tobacco accessions to mount the harpin-mediated HR. We conclude that the predicted receptor-like kinase WAK2 is required for the strong HR induced in tobacco leaves by harpin protein infiltration and can potentially mediate resistance to bacterial pathogens based on harpin recognition.</p>","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 5","pages":"e70096"},"PeriodicalIF":4.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089992/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144110791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Potato StNAC2-StSABP2 Module Enhanced Resistance to Phytophthora infestans Through Activating the Salicylic Acid Pathway.","authors":"Jie Yan, Qian Li, Deying Geng, Zheng Wang, Dongmei Zhao, Dai Zhang, Jinhui Wang, Yang Pan, Jiehua Zhu, Zhihui Yang","doi":"10.1111/mpp.70081","DOIUrl":"https://doi.org/10.1111/mpp.70081","url":null,"abstract":"<p><p>Potato late blight is an important disease in potato production, which causes serious damage. Salicylic acid (SA) is a plant hormone involved in the regulation of potato (Solanum tuberosum) resistance to Phytophthora infestans. In this study, it was found that exogenous methyl salicylate (MeSA) treatment could significantly enhance the resistance of potato to P. infestans. RNA-seq results confirmed that SA was important for potato resistance to P. infestans. Salicylic acid binding protein 2 (SABP2) is a member of α/β hydrolase family, which can convert MeSA into SA to regulate the steady state of SA in plants. StSABP2 protein was obtained through prokaryotic expression, and enzymatic analysis in vitro confirmed that StSABP2 could transform MeSA into SA. In order to explore the function of StSABP2 in the process of plant resistance to P. infestans, we carried out virus-mediated gene silencing of StSABP2 in potato and transiently expressed StSABP2 in tobacco. The results showed that StSABP2 positively regulated plant resistance to P. infestans, and this process was achieved by mediating the transcription of SA signal and defence-related genes. Then we screened for the upstream regulator of StSABP2. The results of double luciferase and yeast one-hybrid analysis showed that StNAC2 could activate the transcription of StSABP2. The StNAC2-StSABP2 module regulated potato resistance to P. infestans by positively mediating the SA pathway. This study provides a new idea for improving host resistance to potato late blight by regulating the SA signal in potato and provides germplasm resources for potato resistance breeding.</p>","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 5","pages":"e70081"},"PeriodicalIF":4.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12040442/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144028206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clavibacter michiganensis Reframed: The Story of How the Genomics Era Made a New Face for an Old Enemy.","authors":"Ebrahim Osdaghi, Hamid Abachi, Marie-Agnes Jacques","doi":"10.1111/mpp.70093","DOIUrl":"10.1111/mpp.70093","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;Bacterial wilt and canker of tomato caused by the gram-positive corynebacterial species Clavibacter michiganensis is an economically important disease threatening the tomato industry in both open-air and greenhouse productions around the world. The disease occurs in many countries, with a particular importance in regions characterised by high temperature and water scarcity. Management of bacterial canker has been a major problem since its original description in 1909. This is due in part to the seedborne nature of the pathogen, allowing the bacterium to be transmitted over long distances via infected seeds, as well as a lack of effective treatment to clean seeds. Detection of the pathogen from seeds is difficult due to high competition on culture media with diverse members of the seed-associated microbiota. Identification of the pathogen can also be difficult owing to the presence of different colony variants on culture media. In this review, we provide a historical perspective and an updated overview on the aetiology, epidemiology and management strategies of the bacterial canker disease. We also gathered recent molecular findings in the pathogenicity mechanisms and bioecology of C. michiganensis to boost management of the bacterial canker disease in the 21&lt;sup&gt;st&lt;/sup&gt; century tomato industry.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Taxonomy: &lt;/strong&gt;Class: Actinobacteria; Order: Micrococcales; Family: Microbacteriaceae; Genus: Clavibacter; Species: Clavibacter michiganensis.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Disease symptoms: &lt;/strong&gt;Interveinal leaf chlorosis leading to necrotic areas. Canker on stems and lateral branches of the plant. Discolouration of vascular and pith tissues to dark yellow or brown. Small and early ripened fruits or discolouration of the placenta from white to yellow in the interior part of the ripening fruits.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Host range: &lt;/strong&gt;Tomato (Solanum lycopersicum) is the main host of the pathogen while natural infection has also been reported on eggplant, pepper and wild nightshade plants.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Synonyms (historical/non-preferred scientific names): &lt;/strong&gt;Aplanobacter michiganensis; Pseudomonas michiganense; Pseudomonas michiganensis; Bacterium michiganense; Phytomonas michiganensis; Mycobacterium michiganense; Erwinia michiganensis (=michiganense); Corynebacterium michiganense; Corynebacterium michiganense pv. michiganense; Corynebacterium michiganense subsp. michiganense; Clavibacter michiganensis subsp. michiganensis.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Microbiological properties: &lt;/strong&gt;The bacterium produces domed, round and shiny mucoid colonies on general culture media. Colonies are usually yellow-pigmented, while pink-pigmented strains are occasionally observed. Cells are gram-positive, aerobic, non-motile, non-spore-producing curved rods (coryneform).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Distribution: &lt;/strong&gt;Present in all continents.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Phytosanitary categorization: &lt;/strong&gt;EPPO A2 List no. 50, EU 2019/2072 RNQP Annex IV. See EPPO (","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 5","pages":"e70093"},"PeriodicalIF":4.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089995/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144110787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}