Molecular plant pathology最新文献

The two-component system CpxA/CpxR regulates pathogenesis and stress adaptability in the poplar canker bacterium Lonsdalea populi. 双组分系统 CpxA/CpxR 调节杨树腐烂病菌 Lonsdalea populi 的致病机理和胁迫适应性。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-11-01 DOI: 10.1111/mpp.70029

Ruirui Yang, Zexu Ming, Sha Zeng, Yanwei Wang, Yonglin Wang, Aining Li

{"title":"The two-component system CpxA/CpxR regulates pathogenesis and stress adaptability in the poplar canker bacterium Lonsdalea populi.","authors":"Ruirui Yang, Zexu Ming, Sha Zeng, Yanwei Wang, Yonglin Wang, Aining Li","doi":"10.1111/mpp.70029","DOIUrl":"10.1111/mpp.70029","url":null,"abstract":"Bacteria employ two-component systems (TCSs) to rapidly sense and respond to their surroundings often and during plant infection. Poplar canker caused by Lonsdalea populi is an emerging woody bacterial disease that leads to high mortality and poplar plantation losses in China. Nonetheless, the information about the underlying mechanism of pathogenesis remains scarce. Therefore, in this study, we reported the role of a TCS pair CpxA/CpxR in regulating virulence and stress responses in L. populi. The CpxA/R system is essential during infection, flagellum formation, and oxidative stress response. Specifically, the Cpx system affected flagellum formation by controlling the expression of flagellum-related genes. CpxR, which was activated by phosphorylation in the presence of CpxA, participated in the transcriptional regulation of a chaperone sctU and the type III secretion system (T3SS)-related genes, thereby influencing T3SS functions during L. populi infection. Phosphorylated CpxR directly manipulated the transcription of a membrane protein-coding gene yccA and the deletion of yccA resulted in reduced virulence and increased sensitivity to H2O2. Furthermore, we mutated the conserved phosphorylation site of CpxR and found that CpxRD51A could no longer bind to the yccA promoter but could still bind to the sctU promoter. Together, our findings elucidate the roles of the Cpx system in regulating virulence and reactive oxygen species resistance and provide further evidence that the TCS is crucial during infection and stress response.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 11","pages":"e70029"},"PeriodicalIF":4.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11568244/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Abscisic acid-, stress-, ripening-induced 2 like protein, TaASR2L, promotes wheat resistance to stripe rust. 脱落酸、胁迫、成熟诱导的 2 类蛋白 TaASR2L 促进小麦对条锈病的抗性。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-11-01 DOI: 10.1111/mpp.70028

Qiao Wang, Yaqi Tang, Ying Li, Jun Ren, Hongxu Zuo, Peng Cheng, Qiang Li, Baotong Wang

{"title":"Abscisic acid-, stress-, ripening-induced 2 like protein, TaASR2L, promotes wheat resistance to stripe rust.","authors":"Qiao Wang, Yaqi Tang, Ying Li, Jun Ren, Hongxu Zuo, Peng Cheng, Qiang Li, Baotong Wang","doi":"10.1111/mpp.70028","DOIUrl":"10.1111/mpp.70028","url":null,"abstract":"Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive wheat diseases. The plant hormone abscisic acid (ABA) plays a key regulatory role in plant response to stress. ABA-, stress-, ripening-induced proteins (ASR) have been shown to be abundantly induced in response to biotic and abiotic stresses to protect plants from damage. However, the function of wheat ASR2-like protein (TaASR2L) in plants under biotic stress remains unclear. In this study, transient silencing of TaASR2L using a virus-induced gene silencing system substantially reduced wheat resistance to Pst. TaASR2L interaction with serine/arginine-rich splicing factor SR30-like (TaSR30) was validated mainly in the nucleus. Knockdown of TaSR30 expression substantially reduced wheat resistance to Pst. Overexpression of TaASR2L and TaSR30 demonstrated that they can promote the expression of ABA- and resistance-related genes to enhance wheat resistance to Pst. In addition, the expression levels of TaSR30 and TaASR2L were substantially increased by exogenous ABA, and the resistance of wheat to Pst was increased, and the expression of PR genes was induced. Therefore, these results suggest that TaASR2L interacts with TaSR30 by promoting PR genes expression and enhancing wheat resistance to Pst.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 11","pages":"e70028"},"PeriodicalIF":4.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11551255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142623901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Phytophthora infestans effector Pi05910 suppresses and destabilizes host glycolate oxidase StGOX4 to promote plant susceptibility. Phytophthora infestans效应子Pi05910抑制并破坏宿主乙醇氧化酶StGOX4的稳定性，从而促进植物的易感性。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-11-01 DOI: 10.1111/mpp.70021

Peiling Zhang, Jinyang Li, Xiuhong Gou, Lin Zhu, Yang Yang, Yilin Li, Yingqi Zhang, Liwen Ding, Assiya Ansabayeva, Yuling Meng, Weixing Shan

{"title":"The Phytophthora infestans effector Pi05910 suppresses and destabilizes host glycolate oxidase StGOX4 to promote plant susceptibility.","authors":"Peiling Zhang, Jinyang Li, Xiuhong Gou, Lin Zhu, Yang Yang, Yilin Li, Yingqi Zhang, Liwen Ding, Assiya Ansabayeva, Yuling Meng, Weixing Shan","doi":"10.1111/mpp.70021","DOIUrl":"10.1111/mpp.70021","url":null,"abstract":"Phytophthora infestans is a notorious oomycete pathogen that causes potato late blight. It secretes numerous effector proteins to manipulate host immunity. Understanding mechanisms underlying their host cell manipulation is crucial for developing disease resistance strategies. Here, we report that the conserved RXLR effector Pi05910 of P. infestans is a genotype-specific avirulence elicitor on potato variety Longshu 12 and contributes virulence by suppressing and destabilizing host glycolate oxidase StGOX4. By performing co-immunoprecipitation, yeast-two-hybrid assays, luciferase complementation imaging, bimolecular fluorescence complementation and isothermal titration calorimetry assays, we identified and confirmed potato StGOX4 as a target of Pi05910. Further analysis revealed that StGOX4 and its homologue NbGOX4 are positive immune regulators against P. infestans, as indicated by infection assays on potato and Nicotiana benthamiana overexpressing StGOX4 and TRV-NbGOX4 plants. StGOX4-mediated disease resistance involves enhanced reactive oxygen species accumulation and activated the salicylic acid signalling pathway. Pi05910 binding inhibited enzymatic activity and destabilized StGOX4. Furthermore, mutagenesis analyses indicated that the 25th residue (tyrosine, Y25) of StGOX4 mediates Pi05910 binding and is required for its immune function. Our results revealed that the core RXLR effector of P. infestans Pi05910 suppresses plant immunity by targeting StGOX4, which results in decreased enzymatic activity and protein accumulation, leading to enhanced plant susceptibility.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 11","pages":"e70021"},"PeriodicalIF":4.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11530570/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The pathogenicity-associated regulators participating in the regulatory cascade for RaxSTAB and RaxX in Xanthomonas oryzae pv. oryzae. 黄单胞菌（Xanthomonas oryzae pv. oryzae）中参与 RaxSTAB 和 RaxX 级联调控的致病性相关调控因子。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-11-01 DOI: 10.1111/mpp.70025

Yanan Shao, Guiyu Tang, Jinye Zhang, Jinjia Zhao, Lifang Ruan

{"title":"The pathogenicity-associated regulators participating in the regulatory cascade for RaxSTAB and RaxX in Xanthomonas oryzae pv. oryzae.","authors":"Yanan Shao, Guiyu Tang, Jinye Zhang, Jinjia Zhao, Lifang Ruan","doi":"10.1111/mpp.70025","DOIUrl":"10.1111/mpp.70025","url":null,"abstract":"The RaxX sulfopeptide, secreted via a type Ι secretion system, is crucial for activating XA21-mediated innate immunity in resistant rice lines bearing the XA21 receptor kinase. Certain pathogenicity-associated regulators that control the expression of the raxSTAB-raxX gene cluster have been functionally characterized, but the comprehensive regulatory cascade of RaxSTAB and RaxX in Xanthomonas oryzae pv. oryzae (Xoo) remains incompletely understood. Our investigation revealed that pathogenicity-associated regulators, including HrpG, HrpX, VemR, PhoR, and Clp, form a regulatory cascade governing the expression of the raxSTAB-raxX gene cluster. HrpG regulates the raxSTAB-raxX gene cluster transcription through the key regulator HrpX. VemR also participates in the transcription of the raxSTAB-raxX. The histidine kinase PhoR positively modulates raxSTAB-raxX expression, while the global regulator Clp directly binds the raxX promoter region to promote its transcription. These findings shed light on the intricate regulatory cascade of rax-related genes in Xoo, emphasizing the complex roles of pathogenicity-associated regulators within the pathogenic regulatory system.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 11","pages":"e70025"},"PeriodicalIF":4.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11554876/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142623906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transformation-based gene silencing and functional characterization of an ISC effector reveal how a powdery mildew fungus disturbs salicylic acid biosynthesis and immune response in the plant. 基于转化的基因沉默和 ISC 效应子的功能表征揭示了白粉病真菌如何干扰水杨酸的生物合成和植物的免疫反应。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-11-01 DOI: 10.1111/mpp.70030

Jinyao Yin, Xiao Li, Linpeng Dong, Xuehuan Zhu, Yalong Chen, Wenyuan Zhao, Yuhan Liu, Jiaxin Shan, Wenbo Liu, Chunhua Lin, Weiguo Miao

{"title":"Transformation-based gene silencing and functional characterization of an ISC effector reveal how a powdery mildew fungus disturbs salicylic acid biosynthesis and immune response in the plant.","authors":"Jinyao Yin, Xiao Li, Linpeng Dong, Xuehuan Zhu, Yalong Chen, Wenyuan Zhao, Yuhan Liu, Jiaxin Shan, Wenbo Liu, Chunhua Lin, Weiguo Miao","doi":"10.1111/mpp.70030","DOIUrl":"10.1111/mpp.70030","url":null,"abstract":"Obligate biotrophic powdery mildew fungi infect a wide range of economically important plants. These fungi often deliver effector proteins into the host tissues to suppress plant immunity and sustain infection. The phytohormone salicylic acid (SA) is one of the most important signals that activate plant immunity against pathogens. However, how powdery mildew effectors interact with host SA signalling is poorly understood. Isochorismatase (ISC) effectors from two other filamentous pathogens have been found to inhibit host SA biosynthesis by hydrolysing isochorismate, the main SA precursor in the plant cytosol. Here, we identified an ISC effector, named EqIsc1, from the rubber tree powdery mildew fungus Erysiphe quercicola. In ISC enzyme assays, EqIsc1 displayed ISC activity by transferring isochorismate to 2,3-dihydro-2,3-dihydroxybenzoate in vitro and in transgenic Nicotiana benthamiana plants. In EqIsc1-expressing transgenic Arabidopsis thaliana, SA biosynthesis and SA-mediated immune response were significantly inhibited. In addition, we developed an electroporation-mediated transformation method for the genetic manipulation of E. quercicola. Inoculation of rubber tree leaves with EqIsc1-silenced E. quercicola strain induced SA-mediated immunity. We also detected the translocation of EqIsc1 into the plant cytosol during the interaction between E. quercicola and its host. Taken together, our results suggest that a powdery mildew effector functions as an ISC enzyme to hydrolyse isochorismate in the host cytosol, altering the SA biosynthesis and immune response.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 11","pages":"e70030"},"PeriodicalIF":4.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142667830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flg22-facilitated PGPR colonization in root tips and control of root rot. Flg22 促进 PGPR 在根尖定殖并控制根腐病。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-11-01 DOI: 10.1111/mpp.70026

Yanan Li, Yafei Li, Yuepeng Wang, Yanqing Yang, Man Qi, Tongfu Su, Rui Li, Dehai Liu, Yuqian Gao, Yuancheng Qi, Liyou Qiu

{"title":"Flg22-facilitated PGPR colonization in root tips and control of root rot.","authors":"Yanan Li, Yafei Li, Yuepeng Wang, Yanqing Yang, Man Qi, Tongfu Su, Rui Li, Dehai Liu, Yuqian Gao, Yuancheng Qi, Liyou Qiu","doi":"10.1111/mpp.70026","DOIUrl":"10.1111/mpp.70026","url":null,"abstract":"Plant root border cells (RBCs) prevent the colonization of plant growth-promoting rhizobacteria (PGPR) at the root tip, rendering the PGPR unable to effectively control pathogens infecting the root tip. In this study, we engineered four strains of Pseudomonas sp. UW4, a typical PGPR strain, each carrying an enhanced green fluorescent protein (EGFP)-expressing plasmid. The UW4E strain harboured only the plasmid, whereas the UW4E-flg22 strain expressed a secreted EGFP-Flg22 fusion protein, the UW4E-Flg(flg22) strain expressed a non-secreted Flg22, and the UW4E-flg22-D strain expressed a secreted Flg22-DNase fusion protein. UW4E-flg22 and UW4E-flg22-D, which secreted Flg22, induced an immune response in wheat RBCs and colonized wheat root tips, whereas the other strains, which did not secrete Flg22, failed to elicit this response and did not colonize wheat root tips. The immune response revealed that wheat RBCs synthesized mucilage, extracellular DNA, and reactive oxygen species. Furthermore, the Flg22-secreting strains showed a 33.8%-93.8% higher colonization of wheat root tips and reduced the root rot incidence caused by Rhizoctonia solani and Fusarium pseudograminearum by 24.6%-35.7% compared to the non-Flg22-secreting strains in pot trials. There was a negative correlation between the incidence of wheat root rot and colonization of wheat root tips by these strains. In contrast, wheat root length and dry weight were positively correlated with the colonization of wheat root tips by these strains. These results demonstrate that engineered secretion of Flg22 by PGPR is an effective strategy for controlling root rot and improving plant growth.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 11","pages":"e70026"},"PeriodicalIF":4.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11534644/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142575777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Herbicides as fungicides: Targeting heme biosynthesis in the maize pathogen Ustilago maydis. 作为杀菌剂的除草剂：以玉米病原体 Ustilago maydis 的血红素生物合成为目标。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-11-01 DOI: 10.1111/mpp.70007

Djihane Damoo, Matthias Kretschmer, Christopher W J Lee, Cornelia Herrfurth, Ivo Feussner, Kai Heimel, James W Kronstad

{"title":"Herbicides as fungicides: Targeting heme biosynthesis in the maize pathogen Ustilago maydis.","authors":"Djihane Damoo, Matthias Kretschmer, Christopher W J Lee, Cornelia Herrfurth, Ivo Feussner, Kai Heimel, James W Kronstad","doi":"10.1111/mpp.70007","DOIUrl":"10.1111/mpp.70007","url":null,"abstract":"Pathogens must efficiently acquire nutrients from host tissue to proliferate, and strategies to block pathogen access therefore hold promise for disease control. In this study, we investigated whether heme biosynthesis is an effective target for ablating the virulence of the phytopathogenic fungus Ustilago maydis on maize plants. We first constructed conditional heme auxotrophs of the fungus by placing the heme biosynthesis gene hem12 encoding uroporphyrinogen decarboxylase (Urod) under the control of nitrogen or carbon source-regulated promoters. These strains were heme auxotrophs under non-permissive conditions and unable to cause disease in maize seedlings, thus demonstrating the inability of the fungus to acquire sufficient heme from host tissue to support proliferation. Subsequent experiments characterized the role of endocytosis in heme uptake, the susceptibility of the fungus to heme toxicity as well as the transcriptional response to exogenous heme. The latter RNA-seq experiments identified a candidate ABC transporter with a role in the response to heme and xenobiotics. Given the importance of heme biosynthesis for U. maydis pathogenesis, we tested the ability of the well-characterized herbicide BroadStar to influence disease. This herbicide contains the active ingredient flumioxazin, an inhibitor of Hem14 in the heme biosynthesis pathway, and we found that it was an effective antifungal agent for blocking disease in maize. Thus, repurposing herbicides for which resistant plants are available may be an effective strategy to control pathogens and achieve crop protection.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 11","pages":"e70007"},"PeriodicalIF":4.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11530707/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The TOR signalling pathway in fungal phytopathogens: A target for plant disease control. 真菌植物病原体中的 TOR 信号通路：植物病害控制的目标

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-11-01 DOI: 10.1111/mpp.70024

Yun Song, Yaru Wang, Huafang Zhang, Muhammad Abu Bakar Saddique, Xiumei Luo, Maozhi Ren

{"title":"The TOR signalling pathway in fungal phytopathogens: A target for plant disease control.","authors":"Yun Song, Yaru Wang, Huafang Zhang, Muhammad Abu Bakar Saddique, Xiumei Luo, Maozhi Ren","doi":"10.1111/mpp.70024","DOIUrl":"10.1111/mpp.70024","url":null,"abstract":"Plant diseases caused by fungal phytopathogens have led to significant economic losses in agriculture worldwide. The management of fungal diseases is mainly dependent on the application of fungicides, which are not suitable for sustainable agriculture, human health, and environmental safety. Thus, it is necessary to develop novel targets and green strategies to mitigate the losses caused by these pathogens. The target of rapamycin (TOR) complexes and key components of the TOR signalling pathway are evolutionally conserved in pathogens and closely related to the vegetative growth and pathogenicity. As indicated in recent systems, chemical, genetic, and genomic studies on the TOR signalling pathway, phytopathogens with TOR dysfunctions show severe growth defects and nonpathogenicity, which makes the TOR signalling pathway to be developed into an ideal candidate target for controlling plant disease. In this review, we comprehensively discuss the current knowledge on components of the TOR signalling pathway in microorganisms and the diverse roles of various plant TOR in response to plant pathogens. Furthermore, we analyse a range of disease management strategies that rely on the TOR signalling pathway, including genetic modification technologies and chemical controls. In the future, disease control strategies based on the TOR signalling network are expected to become a highly effective weapon for crop protection.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 11","pages":"e70024"},"PeriodicalIF":4.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11541241/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142591344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel protein elicitor (Cs08297) from Ciboria shiraiana enhances plant disease resistance. 一种来自 Ciboria shiraiana 的新型蛋白诱导剂（Cs08297）可增强植物的抗病性。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-11-01 DOI: 10.1111/mpp.70023

Shuai Zhang, Ruolan Li, Wei Fan, Xuefei Chen, Caiquan Tao, Shuman Liu, Panpan Zhu, Shuchang Wang, Aichun Zhao

{"title":"A novel protein elicitor (Cs08297) from Ciboria shiraiana enhances plant disease resistance.","authors":"Shuai Zhang, Ruolan Li, Wei Fan, Xuefei Chen, Caiquan Tao, Shuman Liu, Panpan Zhu, Shuchang Wang, Aichun Zhao","doi":"10.1111/mpp.70023","DOIUrl":"10.1111/mpp.70023","url":null,"abstract":"Ciboria shiraiana is a necrotrophic fungus that causes mulberry sclerotinia disease resulting in huge economic losses in agriculture. During infection, the fungus uses immunity elicitors to induce plant tissue necrosis that could facilitate its colonization on plants. However, the key elicitors and immune mechanisms remain unclear in C. shiraiana. Herein, a novel elicitor Cs08297 secreted by C. shiraiana was identified, and it was found to target the apoplast in plants to induce cell death. Cs08297 is a cysteine-rich protein unique to C. shiraiana, and cysteine residues in Cs08297 were crucial for its ability to induce cell death. Cs08297 induced a series of defence responses in Nicotiana benthamiana, including the burst of reactive oxygen species (ROS), callose deposition, and activation of defence-related genes. Cs08297 induced-cell death was mediated by leucine-rich repeat (LRR) receptor-like kinases BAK1 and SOBIR1. Purified His-tagged Cs08297-thioredoxin fusion protein triggered cell death in different plants and enhanced plant resistance to diseases. Cs08297 was necessary for sclerotial development, oxidative-stress adaptation, and cell wall integrity but negatively regulated virulence of C. shiraiana. In conclusion, our results revealed that Cs08297 is a novel fungal elicitor in fungi inducing plant immunity. Furthermore, its potential to enhance plant resistance provides a new target to control agricultural diseases biologically.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 11","pages":"e70023"},"PeriodicalIF":4.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11534627/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142575675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanism of zju-miR156c-mediated network in regulating witches' broom symptom of Chinese jujube. zju-miR156c介导的网络调控大枣 "巫婆帚 "症状的机制

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-11-01 DOI: 10.1111/mpp.70031

Yunjie Wang, Zhi Luo, Xuan Zhao, Hongqiang Sun, Jiaxin Liu, Dongfeng Zhang, Haonan Cao, Changfeng Ai, Lihu Wang, Li Dai, Mengjun Liu, Lixin Wang, Zhiguo Liu

{"title":"Mechanism of zju-miR156c-mediated network in regulating witches' broom symptom of Chinese jujube.","authors":"Yunjie Wang, Zhi Luo, Xuan Zhao, Hongqiang Sun, Jiaxin Liu, Dongfeng Zhang, Haonan Cao, Changfeng Ai, Lihu Wang, Li Dai, Mengjun Liu, Lixin Wang, Zhiguo Liu","doi":"10.1111/mpp.70031","DOIUrl":"10.1111/mpp.70031","url":null,"abstract":"Jujube witches' broom, caused by phytoplasma, is a destructive disease of Chinese jujube. Studies have shown that zju-miR156s play an important role in phytoplasma infection in jujube, but the regulatory mechanism between zju-miR156c and witches' broom remains unexplored. In the current study, miRNA-seq and gene expression analysis showed that zju-miR156c was more highly induced in infected jujube plants than the other miRNAs and its target gene was ZjSPL3. In addition, the expression levels of thymidylate kinase gene (TMKJWB) and secreted jujube protein (SJP1JWB) in diseased materials were higher than those in healthy controls. The expression level of zju-miR156c was significantly upregulated, while ZjSPL3 was sharply downregulated and the content of cytokinin (CTK) significantly increased. Overexpression of zju-miR156c in Arabidopsis significantly reduced the expression of AtSPL10 (homologous gene of ZjSPL3) but increased the content of CTK, and the transgenic plants exhibited witches' broom symptoms. In addition, yeast two-hybrid and co-immunoprecipitation assays confirmed that SJP1JWB interacted with ZjERF18. Yeast one-hybrid analysis showed that ZjERF18 could interact with the promoter of zju-MIR156c. In conclusion, our results demonstrated a novel pathogenic module of ZjERF18-zju-miR156c-ZjSPL3-CTK has an important function in the formation of witches' broom caused by SJP1JWB.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 11","pages":"e70031"},"PeriodicalIF":4.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142667823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0