Molecular plant pathology最新文献

PthXo2B Orthologue Tal7 of Xanthomonas oryzae pv. oryzae Strain IX-221 Acts as a Major Virulence Factor in Indica Rice Without Activating a Clade III SWEET Gene. 黄单胞菌（Xanthomonas oryzae pv. oryzae）菌株 IX-221 的 PthXo2B 直向同源物 Tal7 是籼稻的主要毒性因子，但不激活支系 III SWEET 基因。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-03-01 DOI: 10.1111/mpp.70067

Prashant Mishra, S Shakespear, Sara C D Carpenter, S Hamsa, S Vigi, K N Anith, Prasanta K Dash, Adam J Bogdanove, Rhitu Rai

{"title":"PthXo2B Orthologue Tal7 of Xanthomonas oryzae pv. oryzae Strain IX-221 Acts as a Major Virulence Factor in Indica Rice Without Activating a Clade III SWEET Gene.","authors":"Prashant Mishra, S Shakespear, Sara C D Carpenter, S Hamsa, S Vigi, K N Anith, Prasanta K Dash, Adam J Bogdanove, Rhitu Rai","doi":"10.1111/mpp.70067","DOIUrl":"10.1111/mpp.70067","url":null,"abstract":"In rice bacterial blight, Xanthomonas oryzae pv. oryzae deploys transcription activator-like effectors (TALEs) that upregulate host susceptibility genes. Thirty-four amino acid repeats in TALEs each specify a base in the DNA target, via a repeat-variable diresidue (RVD; positions 12 and 13). Some aberrant-length repeats can disengage to accommodate single base deletions. Clade III SWEET genes SWEET11, -13 and -14 are major susceptibility targets of different TALEs. xa13 is a SWEET11 allele lacking the TALE binding site and thus confers resistance to some strains. It has been deployed widely in India. We report that an xa13-breaking Indian isolate, IX-221, harbours one SWEET14- and two SWEET13-activating TALEs, with one or two disengageable repeats. One, Tal7, orthologous to PthXo2B of Philippines strain PXO61 but with minor, non-RVD sequence differences, like PthXo2B upregulates SWEET13 in a japonica variety and no clade III SWEET in an indica, yet unlike PthXo2B renders both varieties susceptible. A designer TALE with distinct, minor differences also failed to render the indica susceptible. The results suggest that Tal7 activates an alternative susceptibility gene and that non-RVD polymorphism can affect TALE targeting. Moreover, IX-221 provides evidence that the deployment of xa13 in India resulted in strains super-equipped with TALEs that break it.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 3","pages":"e70067"},"PeriodicalIF":4.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11868982/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Regulators and Their Epistatic Networks in Arabidopsis' Defence Responses to Alternaria alternata Infection.

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-02-01 DOI: 10.1111/mpp.70058

Qi Zeng, Xifan Liu, Xuemei Yan, Jiahao Zhang, Chao Li, Chengtai Yan, Yanfeng Zhang, Daniel Kliebenstein, Baohua Li

{"title":"Novel Regulators and Their Epistatic Networks in Arabidopsis' Defence Responses to Alternaria alternata Infection.","authors":"Qi Zeng, Xifan Liu, Xuemei Yan, Jiahao Zhang, Chao Li, Chengtai Yan, Yanfeng Zhang, Daniel Kliebenstein, Baohua Li","doi":"10.1111/mpp.70058","DOIUrl":"10.1111/mpp.70058","url":null,"abstract":"Necrotrophic pathogens cause serious threats to agricultural crops, and understanding the resistance genes and their genetic networks is key to breeding new plant cultivars with better resistance traits. Although Alternaria alternata causes black spot in important leafy brassica vegetables, and leads to significant loss of yield and food quality, little is known about plant-A. alternata interactions. In this study, we used a unique and large collection of single, double and triple mutant lines of defence metabolite regulators in Arabidopsis to explore how these transcription factors and their epistatic networks may influence A. alternata infections. This identified nine novel regulators and 20 pairs of epistatic interactions that modulate Arabidopsis plants' defence responses to A. alternata infection. We further showed that the glucosinolate 4-methoxy-indol-3-ylmethyl is the only glucosinolate consistently responsive to A. alternata infection in Col-0 ecotype. With the further exploration of the regulators and the genetic networks on modulating the accumulation of glucosinolates under A. alternata infection, an inverted triangle regulatory model was proposed for Arabidopsis plants' defence responses at a metabolic level and a phenotypic level.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 2","pages":"e70058"},"PeriodicalIF":4.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11788323/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and Application of the Heptad Repeat Domain in the CPR5 Protein for Enhancing Plant Immunity.

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-02-01 DOI: 10.1111/mpp.70059

Yuehui Zhang, Yuting Ge, Keke Sun, Leiwen Pan, Zhilin Liang, Ping Wang, Yingfan Cai, Shui Wang

{"title":"Identification and Application of the Heptad Repeat Domain in the CPR5 Protein for Enhancing Plant Immunity.","authors":"Yuehui Zhang, Yuting Ge, Keke Sun, Leiwen Pan, Zhilin Liang, Ping Wang, Yingfan Cai, Shui Wang","doi":"10.1111/mpp.70059","DOIUrl":"10.1111/mpp.70059","url":null,"abstract":"Plant resistance to pathogens can be significantly enhanced through genetic modification, thereby reducing the reliance on chemical pesticides. CONSTITUTIVE EXPRESSER OF PATHOGENESIS-RELATED GENES 5 (CPR5) serves as a key negative regulator of plant immunity. Here we explored the functional domains of the CPR5 protein with the goal of dampening its activity to bolster plant immunity. Using hexapeptide asparagine-alanine-alanine-isoleucine-arginine-serine (NAAIRS) linker-scanning analysis, we identified a heptad repeat domain (HRD) in the middle region of the CPR5 protein, which is highly conserved across the plant kingdom. The HRD is predicted to form an α-helix structure and acts as an interface for CPR5 dimerization. Intriguingly, overexpression of the HRD in Arabidopsis wild-type plants resulted in a phenotype similar to the cpr5 mutant and led to an enhancement of plant immunity, indicating that the introduced HRDs disrupt the native CPR5 dimers, thereby relieving the suppression of plant immunity. Furthermore, expression of the HRD under the control of a pathogen-inducible promoter significantly improved the resistance of cotton plants to Verticillium dahliae, a destructive wilt pathogen affecting cotton production worldwide. These findings suggest that downregulating CPR5 activity by the pathogen-inducible expression of its HRD could be a promising approach for strengthening plant immunity.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 2","pages":"e70059"},"PeriodicalIF":4.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11798864/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143255994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reprogrammed Plant Metabolism During Viral Infections: Mechanisms, Pathways and Implications.

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-02-01 DOI: 10.1111/mpp.70066

Tong Jiang, Tianwen Hao, Wenjing Chen, Chengliang Li, Shuqi Pang, Chenglong Fu, Jie Cheng, Chaobo Zhang, Mansour Ghorbanpour, Shuo Miao

{"title":"Reprogrammed Plant Metabolism During Viral Infections: Mechanisms, Pathways and Implications.","authors":"Tong Jiang, Tianwen Hao, Wenjing Chen, Chengliang Li, Shuqi Pang, Chenglong Fu, Jie Cheng, Chaobo Zhang, Mansour Ghorbanpour, Shuo Miao","doi":"10.1111/mpp.70066","DOIUrl":"10.1111/mpp.70066","url":null,"abstract":"Plant viruses pose a significant threat to global agriculture, leading to substantial crop losses that jeopardise food security and disrupt ecosystem stability. These viral infections often reprogramme plant metabolism, compromising key pathways critical for growth and defence. For instance, infections by cucumber mosaic virus alter amino acid and secondary metabolite biosynthesis, including flavonoid and phenylpropanoid pathways, thereby weakening plant defences. Similarly, tomato bushy stunt virus disrupts lipid metabolism by altering the synthesis and accumulation of sterols and phospholipids, which are essential for viral replication and compromise membrane integrity. Recent advancements in gene-editing technologies, such as CRISPR/Cas9, and metabolomics offer innovative strategies to mitigate these impacts. Precise genetic modifications can restore or optimise disrupted metabolic pathways, enhancing crop resilience to viral infections. Metabolomics further aids in identifying metabolic biomarkers linked to viral resistance, guiding breeding programmes aimed at developing virus-resistant plants. By reducing the susceptibility of crops to viral infections, these approaches hold significant potential to reduce dependence on chemical pesticides, increase crop yields and promote sustainable agricultural practices. Future research should focus on expanding our understanding of virus-host interactions at the molecular level while exploring the long-term ecological impacts of viral infections. Interdisciplinary approaches integrating multi-omics technologies and sustainable management strategies will be critical in addressing the challenges posed by plant viruses and ensuring global agricultural stability.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 2","pages":"e70066"},"PeriodicalIF":4.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11839395/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143458666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-Invasive, Bioluminescence-Based Visualisation and Quantification of Bacterial Infections in Arabidopsis Over Time.

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-02-01 DOI: 10.1111/mpp.70055

Nanne W Taks, Mathijs D Batstra, Ronald F Kortekaas, Floris D Stevens, Sebastian Pfeilmeier, Harrold A van den Burg

{"title":"Non-Invasive, Bioluminescence-Based Visualisation and Quantification of Bacterial Infections in Arabidopsis Over Time.","authors":"Nanne W Taks, Mathijs D Batstra, Ronald F Kortekaas, Floris D Stevens, Sebastian Pfeilmeier, Harrold A van den Burg","doi":"10.1111/mpp.70055","DOIUrl":"10.1111/mpp.70055","url":null,"abstract":"Plant-pathogenic bacteria colonise their hosts using various strategies, exploiting both natural openings and wounds in leaves and roots. The vascular pathogen Xanthomonas campestris pv. campestris (Xcc) enters its host through hydathodes, organs at the leaf margin involved in guttation. Subsequently, Xcc breaches the hydathode-xylem barrier and progresses into the xylem vessels causing systemic disease. To elucidate the mechanisms that underpin the different stages of an Xcc infection, a need exists to image bacterial progression in planta in a non-invasive manner. Here, we describe a phenotyping setup and Python image analysis pipeline for capturing 16 independent Xcc infections in Arabidopsis thaliana plants in parallel over time. The setup combines an RGB camera for imaging disease symptoms and an ultrasensitive CCD camera for monitoring bacterial progression inside leaves using bioluminescence. The method reliably quantified bacterial growth in planta for two bacterial species, that is, vascular Xcc and the mesophyll pathogen Pseudomonas syringae pv. tomato (Pst). The camera resolution allowed Xcc imaging already in the hydathodes, yielding reproducible data for the first stages prior to the systemic infection. Data obtained through the image analysis pipeline was robust and validated findings from other bioluminescence imaging methods, while requiring fewer samples. Moreover, bioluminescence was reliably detected within 5 min, offering a significant time advantage over our previously reported method with light-sensitive films. Thus, this method is suitable to quantify the resistance level of a large number of Arabidopsis thaliana accessions and mutant lines to different bacterial strains in a non-invasive manner for phenotypic screenings.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 2","pages":"e70055"},"PeriodicalIF":4.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11788312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Puccinia striiformis Effector PNPi Suppresses TaIAA14 Expression to Inhibit Host Cell Death Response.

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-02-01 DOI: 10.1111/mpp.70063

Huiyutang Wang, Xue Gao, Yixi Kong, Zhiqiang Jia, Liang Qiao, Baoju Yang

{"title":"Puccinia striiformis Effector PNPi Suppresses TaIAA14 Expression to Inhibit Host Cell Death Response.","authors":"Huiyutang Wang, Xue Gao, Yixi Kong, Zhiqiang Jia, Liang Qiao, Baoju Yang","doi":"10.1111/mpp.70063","DOIUrl":"10.1111/mpp.70063","url":null,"abstract":"Fungal pathogens secrete effectors that suppress the hypersensitive response (HR) of the host, characterised by programmed cell death, facilitating colonisation. However, how effectors manipulate host cell death remains poorly understood. In this study, we discovered that the Puccinia striiformis effector PNPi (Puccinia NPR1 interactor) suppressed BAX-induced cell death in Nicotiana benthamiana. This virulence was mediated by the FtsN domain of PNPi, and an enhanced suppression effect was observed when Ser129 was mutated into arginine. Further RNA-sequencing analysis revealed that auxin signalling was disturbed, with the auxin-responsive protein IAA29-like (NbIAA29) being downregulated during cell death suppression by PNPi. Exogenous application of auxins alleviated cell death suppression in N. benthamiana. Silencing NbIAA29 enhanced the PNPi-induced suppression; however, this effect was reduced in NbIAA29-silenced plants pretreated with auxins. Additionally, we confirmed the in vivo interaction between PNPi and TaIAA14, which is the homologous gene of NbIAA29 in wheat. Knocking down TaIAA14 through virus-induced gene silencing significantly increased the fungal development and reduced wheat cell death response. Overall, these results indicate that the P. striiformis effector PNPi suppresses the cell death response by targeting TaIAA14 to facilitate infection, advancing our understanding of how P. striiformis effectors manipulate host immunity and providing a theoretical basis for new strategies of sustainable disease control.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 2","pages":"e70063"},"PeriodicalIF":4.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11842466/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Wheat NLR Protein PM3b Localises to Endoplasmic Reticulum-Plasma Membrane Contact Sites and Interacts With AVRPM3^b2/c2 Through Its LRR Domain.

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-02-01 DOI: 10.1111/mpp.70054

Jonatan Isaksson, Lukas Kunz, Simon Flückiger, Victoria Widrig, Beat Keller

{"title":"The Wheat NLR Protein PM3b Localises to Endoplasmic Reticulum-Plasma Membrane Contact Sites and Interacts With AVRPM3b2/c2 Through Its LRR Domain.","authors":"Jonatan Isaksson, Lukas Kunz, Simon Flückiger, Victoria Widrig, Beat Keller","doi":"10.1111/mpp.70054","DOIUrl":"10.1111/mpp.70054","url":null,"abstract":"Plant nucleotide-binding leucine-rich repeat (NLR) proteins are intracellular immune receptors that directly or indirectly perceive pathogen-derived effector proteins to induce an immune response. NLRs display diverse subcellular localisations, which are associated with the capacity of the immune receptor to confer disease resistance and recognise its corresponding avirulence effector. In wheat, the NLR PM3b recognises the wheat powdery mildew effector AVRPM3b2/c2 and we examined the molecular mechanism underlying this recognition. We show that PM3b and other PM3 variants localise to endoplasmic reticulum (ER)-plasma membrane (PM) contact sites (EPCS), while AVRPM3b2/c2 localises to the nucleocytoplasmic space. Additionally, we found that PM3b interacts in planta with AVRPM3b2/c2 through its LRR domain. We further demonstrate that full-length PM3b interaction with AVRPM3b2/c2 is considerably weaker than for the isolated PM3b LRR domain or the susceptible PM3 variant PM3CS, indicating that activation of PM3b leads to dissociation of the complex. In line with this, we observed a strong interaction between PM3b and AVRPM3b2/c2 in a P-loop mutant of PM3b that was unable to initiate a cell death response, or when an inactive variant of AVRPM3b2/c2 was used. We propose that PM3b transiently interacts with AVRPM3b2/c2 through residues in the LRR that are conserved among PM3 variants, while the amino acids necessary for full activation and cell death signalling are unique to PM3b. Our data suggests that PM3b localisation and interaction with AVRPM3b2/c2 differ from other well-studied NLRs and further highlights the mechanistic diversity in NLR-mediated responses against pathogens in plants.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 2","pages":"e70054"},"PeriodicalIF":4.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11799908/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143255998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

"Candidatus Liberibacter asiaticus" Infection Induces Citric Acid Accumulation and Immune Responses Mediated by the Transcription Factor CitPH4.

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-02-01 DOI: 10.1111/mpp.70062

Bin Hu, Tao Yuan, Zhihao Lu, Rongyan Huang, Jiaxian He, Kun Yang, Qinchun Wu, Wanqi Ai, Wang Zhang, Weikang Zheng, Xiaoxiao Wu, Xia Wang, Yuantao Xu, Xiuxin Deng, Qiang Xu

{"title":"\"Candidatus Liberibacter asiaticus\" Infection Induces Citric Acid Accumulation and Immune Responses Mediated by the Transcription Factor CitPH4.","authors":"Bin Hu, Tao Yuan, Zhihao Lu, Rongyan Huang, Jiaxian He, Kun Yang, Qinchun Wu, Wanqi Ai, Wang Zhang, Weikang Zheng, Xiaoxiao Wu, Xia Wang, Yuantao Xu, Xiuxin Deng, Qiang Xu","doi":"10.1111/mpp.70062","DOIUrl":"10.1111/mpp.70062","url":null,"abstract":"Citrus huanglongbing (HLB), caused by \"Candidatus Liberibacter\" spp., is one of the most disastrous citrus diseases worldwide. HLB-affected citrus fruits are significantly more acidic than healthy fruits. However, the molecular mechanism behind this phenomenon remains to be elucidated. Here, we report that HLB-affected fruits have higher levels of citric acid (CA) than healthy fruits. Moreover, Citrus PH4 (CitPH4), which encodes a MYB transcription factor that functions as a key regulator of CA accumulation, was upregulated in HLB-affected fruits relative to healthy fruits. Heterologous overexpression of CitPH4 in tobacco (Nicotiana tabacum) plants enhanced tolerance to HLB. Subsequently, overexpression and gene-editing experiments indicated that CitPH4 can affect the salicylic acid (SA) pathway, which directly binds to and activates the promoter of CsPBS3, a key gene of SA biosynthesis. HLB-affected fruits had higher SA levels than healthy fruits. Furthermore, application of SA activated CA biosynthesis and application of CA activated SA biosynthesis and signalling in citrus fruits and decreased \"Candidatus Liberibacter asiaticus\" (CLas) titres in infected leaves. This work suggests that CitPH4 is a key node between CA and SA, thus revealing crosstalk between defence responses and fruit quality in citrus.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 2","pages":"e70062"},"PeriodicalIF":4.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11821725/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143408576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Constructing a Novel Disease Resistance Mechanism Model for Cruciferous Crops: An Example From Black Rot.

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-02-01 DOI: 10.1111/mpp.70060

Haojie Dai, Linli Hu, Jie Wang, Zhibin Yue, Jue Wang, Tongyan Chen, Jinbao Li, Tingting Dou, Jihua Yu, Zeci Liu

{"title":"Constructing a Novel Disease Resistance Mechanism Model for Cruciferous Crops: An Example From Black Rot.","authors":"Haojie Dai, Linli Hu, Jie Wang, Zhibin Yue, Jue Wang, Tongyan Chen, Jinbao Li, Tingting Dou, Jihua Yu, Zeci Liu","doi":"10.1111/mpp.70060","DOIUrl":"10.1111/mpp.70060","url":null,"abstract":"Cruciferous crops are essential components of global agricultural production due to their rich nutritional value and extensive economic benefits. Black rot caused by Xanthomonas campestris pv. campestris (Xcc) has caused significant losses to cruciferous crops. Therefore, studying the resistance mechanisms of cruciferous crops to improve the disease resistance of cruciferous crops is of significant practical importance. This review introduces the biological characteristics and epidemiological patterns of the Xcc. The main resistance mechanisms including the physical barrier functions, immune responses, systemic resistance, regulation of photosynthesis, antimicrobial effects of secondary metabolites, production and regulation of reactive oxygen species, and the signalling pathways of salicylic acid, jasmonic acid and ethylene of cruciferous crops to Xcc are also summarised. Comprehensive knowledge of these resistance mechanisms will provide theoretical support for enhancing disease resistance in crops.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 2","pages":"e70060"},"PeriodicalIF":4.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11808048/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143382647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MdVQ37 Negatively Regulates Apple Resistance to Valsa Canker via SA-Dependent and SA-Independent Pathways.

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-02-01 DOI: 10.1111/mpp.70064

Dingyue Duan, Wenqian Zheng, Mingrui Shi, Ran Yi, Qinglong Dong, Jie Yang, Fengwang Ma, Ke Mao

{"title":"MdVQ37 Negatively Regulates Apple Resistance to Valsa Canker via SA-Dependent and SA-Independent Pathways.","authors":"Dingyue Duan, Wenqian Zheng, Mingrui Shi, Ran Yi, Qinglong Dong, Jie Yang, Fengwang Ma, Ke Mao","doi":"10.1111/mpp.70064","DOIUrl":"10.1111/mpp.70064","url":null,"abstract":"Apple Valsa canker is one of the major fungal diseases adversely affecting the apple industry. Valine-glutamine motif-containing proteins (VQs) are a kind of plant transcription regulation cofactor, which are involved in regulating the growth and development of plants and their defence responses. Yet little is known about the role of VQs in the biotic stress response of woody plants, especially in apple trees. Recently, we identified MdVQ37 as a negative regulator of apple resistance to Glomerella leaf spot by inhibiting MdWRKY100-mediated salicylic acid (SA) accumulation via protein interaction. Here we found that MdVQ37 expression was induced significantly by Valsa mali infection. Overexpressing MdVQ37 in apple increased its susceptibility to V. mali, accompanied by a reduction in H2O2 accumulation along with the activity of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), chitinase and β-1,3-glucanase. Meanwhile, MdVQ37 overexpression increased the expression of two SA catabolic genes, MdS5H1 and MdS5H2, resulting in a greater accumulation of the SA metabolite 2,5-dihydroxybenzoic acid (2,5-DHBA), but a decreased SA content and less signalling in transgenic plants. MdPER3, a peroxidase-encoding gene, was identified as a direct target of MdWRKY100. Analysis of transcriptional regulation activity showed that MdVQ37 inhibited the binding and transcriptional ability of MdWRKY100 on the MdPER3 promoter, thereby reducing antioxidant enzyme activity and weakening the Valsa canker resistance. Overall, our results show that MdVQ37 negatively regulates apple defence against V. mali through both SA-dependent and SA-independent pathways. Our findings provide novel insight into the mechanisms by which the VQ-WRKY complex modulates plant defence responses against pathogens.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 2","pages":"e70064"},"PeriodicalIF":4.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11835766/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143449738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0