Molecular plant pathology最新文献

ClBeclin1 Positively Regulates Citrus Defence Against Citrus Yellow Vein Clearing Virus Through Mediating Autophagy-Dependent Degradation of ClAPX1.

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-12-01 DOI: 10.1111/mpp.70041

Jiajun Wang, Ling Yu, Jinfa Zhao, Shimin Fu, Yalin Mei, Binghai Lou, Yan Zhou

{"title":"ClBeclin1 Positively Regulates Citrus Defence Against Citrus Yellow Vein Clearing Virus Through Mediating Autophagy-Dependent Degradation of ClAPX1.","authors":"Jiajun Wang, Ling Yu, Jinfa Zhao, Shimin Fu, Yalin Mei, Binghai Lou, Yan Zhou","doi":"10.1111/mpp.70041","DOIUrl":"10.1111/mpp.70041","url":null,"abstract":"Autophagy, one of the most widespread and highly conserved protein degradation systems in eukaryotic cells, plays an important role in plant growth, development and stress response. Beclin 1 is a core component of the phosphatidylinositol 3-kinase (PI3K) autophagy complex and positively regulates plant immunity against viruses. The upregulation of Eureka lemon ClBeclin1 was observed in response to citrus yellow vein clearing virus (CYVCV) infection. However, the function of ClBeclin1 and the underlying mechanism during CYVCV colonisation remain unclear. Here, the resistance evaluation of the overexpression and silencing of ClBeclin1 in Eureka lemon hairy roots revealed it as a positive regulator of citrus immunity against CYVCV. Transcriptomic profiling and metabolic analyses along with genetic evidence implied that the overexpression of ClBeclin1 positively triggered reactive oxygen species (ROS)- and jasmonic acid (JA)-mediated immunity in citrus. The accumulation of ROS and JA contents was attributed to the autophagic degradation of the ROS scavenger ClAPX1 via ClBeclin1 overexpression. Exogenous application of either H2O2 or JA significantly reduced CYVCV colonisation and vein-clearing symptoms on the host. Collectively, our findings indicate that ClBeclin1 activation contributes to citrus immunity against CYVCV through triggering ROS- and JA-mediated defence responses, and the accumulation of ROS and JA resulted from the autophagic degradation of ClAPX1 by ClBeclin1.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 12","pages":"e70041"},"PeriodicalIF":4.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11631719/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Genomics Reveals Sources of Genetic Variability in the Asexual Fungal Plant Pathogen Colletotrichum lupini. 比较基因组学揭示了无性真菌植物病原体 Colletotrichum lupini 的遗传变异来源。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-12-01 DOI: 10.1111/mpp.70039

Joris A Alkemade, Pierre Hohmann, Monika M Messmer, Timothy G Barraclough

{"title":"Comparative Genomics Reveals Sources of Genetic Variability in the Asexual Fungal Plant Pathogen Colletotrichum lupini.","authors":"Joris A Alkemade, Pierre Hohmann, Monika M Messmer, Timothy G Barraclough","doi":"10.1111/mpp.70039","DOIUrl":"10.1111/mpp.70039","url":null,"abstract":"Fungal plant pathogens cause major crop losses worldwide, with many featuring compartmentalised genomes that include both core and accessory regions, which are believed to drive adaptation. The highly host-specific fungus Colletotrichum lupini greatly impacts lupin (Lupinus spp.) cultivation. This pathogen is part of clade 1 of the C. acutatum species complex and comprises four genetically uniform, presumably clonal, lineages (I-IV). Despite this, variation in virulence and morphology has been observed within these lineages. To investigate the potential sources of genetic variability in this asexual fungus, we compared the genomes of 16 C. lupini strains and 17 related Colletotrichum species. Phylogenomics confirmed the presence of four distinct lineages, but further examination based on genome size, gene content, transposable elements (TEs), and deletions revealed that lineage II could be split into two groups, II-A and II-B. TE content varied between lineages and correlated strongly with genome size variation, supporting a role for TEs in genome expansion in this species. Pangenome analysis revealed a highly variable accessory genome, including a minichromosome present in lineages II, III, and IV, but absent in lineage I. Accessory genes and effectors appeared to cluster in proximity to TEs. Presence/absence variation of putative effectors was lineage-specific, suggesting that these genes play a crucial role in determining host range. Notably, no effectors were found on the TE-rich minichromosome. Our findings shed light on the potential mechanisms generating genetic diversity in this asexual fungal pathogen that could aid future disease management.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 12","pages":"e70039"},"PeriodicalIF":4.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11645255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142822210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

XopM, An FFAT Motif-Containing Type III Effector Protein From Xanthomonas, Suppresses MTI Responses at the Plant Plasma Membrane.

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-12-01 DOI: 10.1111/mpp.70038

Charlotte Brinkmann, Jennifer Bortlik, Margot Raffeiner, Manuel González-Fuente, Linus F Börnke, Suayib Üstün, Frederik Börnke

{"title":"XopM, An FFAT Motif-Containing Type III Effector Protein From Xanthomonas, Suppresses MTI Responses at the Plant Plasma Membrane.","authors":"Charlotte Brinkmann, Jennifer Bortlik, Margot Raffeiner, Manuel González-Fuente, Linus F Börnke, Suayib Üstün, Frederik Börnke","doi":"10.1111/mpp.70038","DOIUrl":"10.1111/mpp.70038","url":null,"abstract":"Many gram-negative pathogenic bacteria use type III effector proteins (T3Es) as essential virulence factors to suppress host immunity and to cause disease. However, in many cases the molecular function of T3Es remains unknown. The plant pathogen Xanthomonas campestris pv. vesicatoria (Xcv) is the causal agent of bacterial spot disease on tomato and pepper plants and is known to translocate around 36 T3Es into its host cell, which collectively suppress plant defence and promote infection. XopM is an Xcv core T3E with unknown function that has no similarity to any other known protein. We found that XopM interacts with vesicle-associated membrane protein (VAMP)-associated proteins (VAPs) in an isoform-specific manner. The endoplasmic reticulum (ER) integral membrane protein VAP is a common component of membrane contact sites involved in both tethering and lipid transfer by binding directly to proteins containing an FFAT (two phenylalanines [FF] in an acidic tract [AT]) motif. Sequence analyses revealed that XopM displays two FFAT motifs that cooperatively mediated the interaction of XopM with VAP. When expressed in plants, XopM supported growth of a nonpathogenic bacterial strain and dampened the production of reactive oxygen species, indicating its ability to suppress plant immunity. Further analyses revealed that the interaction with VAP and the ability to suppress microbe-associated molecular pattern-triggered immunity (MTI) are structurally and functionally separable, although XopM requires localisation to the host membrane system for full MTI suppression activity. We discuss a working model in which XopM uses FFAT motifs to target the membrane to interfere with early MTI responses.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 12","pages":"e70038"},"PeriodicalIF":4.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11631713/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: New persistent plant RNA virus carries mutations to weaken viral suppression of antiviral RNA interference.

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-12-01 DOI: 10.1111/mpp.70035

{"title":"Correction to: New persistent plant RNA virus carries mutations to weaken viral suppression of antiviral RNA interference.","authors":"","doi":"10.1111/mpp.70035","DOIUrl":"10.1111/mpp.70035","url":null,"abstract":"Zhu, L.-J., Zhu, Y., Zou, C., Su, L.-Y., Zhang, C.-T., Wang, C. et al. (2024) New persistent plant RNA virus carries mutations to weaken viral suppression of antiviral RNA interference. Molecular Plant Pathology, 25, e70020. Available from: https://doi.org/10.1111/mpp.70020 The following errors have been identified in the above published article: The co-first authors and co-corresponding authors are not indicated. The order of the funds appearing in the Funding information of the article are not consistent with the order in the Acknowledgements. The authors would like to correct these errors as follows: Li-Juan Zhu and Yu Zhu contributed equally to this work. Jian-Guo Wu and Yan-Hong Han are co-corresponding authors. Jiang-Guo Wu: wujianguo81@126.com; Yan-Hong Han: yan-hong@fafu.edu The correct order of funds is: National Natural Science Foundation of China, Grant/Award Number 32025031 and 31,900,153, National Key Research and Development Program of China, Grant/Award Number 2023YFF1000500 and Special Fund Project for Science and Technology Innovation of FAFU, Grant/Award Number KFB23013. We apologise for these errors.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 12","pages":"e70035"},"PeriodicalIF":4.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11631711/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Specific Transcriptional Regulation Controls Plant Organ-Specific Infection by the Oomycete Pathogen Phytophthora sojae.

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-12-01 DOI: 10.1111/mpp.70042

Long Lin, Yang Wang, Hui Qian, Jiawei Wu, Yachun Lin, Yeqiang Xia, Suomeng Dong, Wenwu Ye, Yuanchao Wang

{"title":"Specific Transcriptional Regulation Controls Plant Organ-Specific Infection by the Oomycete Pathogen Phytophthora sojae.","authors":"Long Lin, Yang Wang, Hui Qian, Jiawei Wu, Yachun Lin, Yeqiang Xia, Suomeng Dong, Wenwu Ye, Yuanchao Wang","doi":"10.1111/mpp.70042","DOIUrl":"10.1111/mpp.70042","url":null,"abstract":"The organs of a plant species vary in cell structure, metabolism and defence responses. However, the mechanisms that enable a single pathogen to colonise different plant organs remain unclear. Here we compared the transcriptome of the oomycete pathogen Phytophthora sojae during infection of roots versus leaves of soybeans. We found differences in the transcript levels of hundreds of pathogenicity-related genes, particularly genes encoding carbohydrate-active enzymes, secreted (effector) proteins, oxidoreductase-related proteins and transporters. To identify the key regulator for root-specific infection, we knocked out root-specific transcription factors (TFs) and found the mutants of PsBZPc29, which encodes a member of an oomycete-specific class of basic leucine zipper (bZIP) TFs, displayed reduced virulence on soybean roots but not on leaves. More than 60% of the root-specific genes showed reduced expression in the mutants during root infection. The results suggest that transcriptional regulation underlies the organ-specific infection by P. sojae, and that a bZIP TF plays a key role in root-specific transcriptional regulation.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 12","pages":"e70042"},"PeriodicalIF":4.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11645254/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142822127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lrp Family Regulator SCAB_Lrp2 Responds to the Precursor Tryptophan and Represses the Thaxtomin Biosynthesis in Streptomyces scabies.

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-12-01 DOI: 10.1111/mpp.70036

Haoyang He, Lijuan Tang, Mingrui Song, Hui Chen, Youquan Zou, Xueyan Li, Endong Yang, Hang Wu, Buchang Zhang, Jing Liu

{"title":"Lrp Family Regulator SCAB_Lrp2 Responds to the Precursor Tryptophan and Represses the Thaxtomin Biosynthesis in Streptomyces scabies.","authors":"Haoyang He, Lijuan Tang, Mingrui Song, Hui Chen, Youquan Zou, Xueyan Li, Endong Yang, Hang Wu, Buchang Zhang, Jing Liu","doi":"10.1111/mpp.70036","DOIUrl":"https://doi.org/10.1111/mpp.70036","url":null,"abstract":"Streptomyces scabies is a well-researched plant pathogen belonging to the genus Streptomyces. Its virulence is linked to the production of the secondary metabolite thaxtomin A, which is tightly regulated at the transcriptional level. The leucine-responsive regulatory protein (Lrp) family is conserved in prokaryotes and is involved in various crucial biological processes. However, the regulatory interaction between Lrp protein and pathogenic Streptomyces species remains poorly understood. This study aims to explore the role of SCAB_Lrp2 in regulating thaxtomin biosynthesis and pathogenicity, and to analyse the shared and unique features of Lrp homologues in S. scabies. We observed that SCAB_Lrp2 (SCAB_75421) showed significant homology with SCAB_Lrp, a recognised activator of thaxtomin A production in S. scabies. Our results revealed a regulatory interaction between SCAB_Lrp2 and SCAB_Lrp in terms of their targets, although SCAB_Lrp2 does not respond to the amino acid-effectors of SCAB_Lrp. In contrast to SCAB_Lrp, deletion of SCAB_Lrp2 resulted in a notable increase in thaxtomin A production with the emergence of a hypervirulent phenotype in S. scabies. Further analysis revealed that SCAB_Lrp2 represses the transcription of the thaxtomin biosynthetic gene cluster by directly regulating the cluster-situated regulator (CSR) gene txtR. Moreover, the precursor of thaxtomin, tryptophan, acts as an effector of SCAB_Lrp2, strengthening the repressive effect on thaxtomin biosynthesis through txtR. These findings provide new insights into the functional conservation and diversity of Lrp homologues involved in the biosynthesis of thaxtomin phytotoxins in pathogenic Streptomyces species.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 12","pages":"e70036"},"PeriodicalIF":4.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11609053/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142770581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to 'Heat Shock Transcription Factor 3 Regulates Plant Immune Response Through Modulation of Salicylic Acid Accumulation and Signalling in Cassava'.

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-12-01 DOI: 10.1111/mpp.70032

引用次数: 0

SsPtc3 Modulating SsSmk1-MAPK and Autophagy to Facilitate Growth and Pathogenicity in Sclerotinia sclerotiorum.

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-12-01 DOI: 10.1111/mpp.70037

Wenli Jiao, Dongmeng Ma, Qi Zuo, Yalan Li, Jun Hu, Dongmei Jia, Yanhua Zhang, Jinliang Liu, Xianghui Zhang, Hongyu Pan

{"title":"SsPtc3 Modulating SsSmk1-MAPK and Autophagy to Facilitate Growth and Pathogenicity in Sclerotinia sclerotiorum.","authors":"Wenli Jiao, Dongmeng Ma, Qi Zuo, Yalan Li, Jun Hu, Dongmei Jia, Yanhua Zhang, Jinliang Liu, Xianghui Zhang, Hongyu Pan","doi":"10.1111/mpp.70037","DOIUrl":"https://doi.org/10.1111/mpp.70037","url":null,"abstract":"The compound appressoria of Sclerotinia sclerotiorum can produce cell wall-degrading enzymes, effectors and toxins, which promote penetration and the death of host cells. Subsequently, invasive hyphae (IH) branch rapidly as necrotrophic growth and disease symptoms are observed. S. sclerotiorum can respond to complex stresses and regulate its metabolism to adapt to the external environment. Here we demonstrated that type 2C Ser/Thr phosphatase (PP2C) SsPtc3 responds to nutritional, osmotic, cell wall and oxidative stresses. Loss of function ΔSsptc3 mutants displayed defects in mycelial growth, sclerotia formation and reduced virulence. Phosphoproteomic analyses revealed that SsPtc3 is involved in autophagy and MAPK signalling pathways. We obtained evidence that SsPtc3 negatively modulates the phosphorylation of SsSmk1. SsSmk1 is essential for mycelial growth, compound appressorium formation and pathogenicity, SsPtc3 modulated phosphorylation homeostasis of SsSmk1 to maintain hyphal growth. SsPtc3 interacted with SsAtg1 to influence autophagic flux under starvation. Taken together, these results reveal that SsPtc3 responds to various stresses that modulate autophagy and phosphorylation of SsSmk1-MAPK, which facilitates the growth and virulence of S. sclerotiorum.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 12","pages":"e70037"},"PeriodicalIF":4.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11609052/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142770584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The two-component system CpxA/CpxR regulates pathogenesis and stress adaptability in the poplar canker bacterium Lonsdalea populi. 双组分系统 CpxA/CpxR 调节杨树腐烂病菌 Lonsdalea populi 的致病机理和胁迫适应性。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-11-01 DOI: 10.1111/mpp.70029

Ruirui Yang, Zexu Ming, Sha Zeng, Yanwei Wang, Yonglin Wang, Aining Li

{"title":"The two-component system CpxA/CpxR regulates pathogenesis and stress adaptability in the poplar canker bacterium Lonsdalea populi.","authors":"Ruirui Yang, Zexu Ming, Sha Zeng, Yanwei Wang, Yonglin Wang, Aining Li","doi":"10.1111/mpp.70029","DOIUrl":"10.1111/mpp.70029","url":null,"abstract":"Bacteria employ two-component systems (TCSs) to rapidly sense and respond to their surroundings often and during plant infection. Poplar canker caused by Lonsdalea populi is an emerging woody bacterial disease that leads to high mortality and poplar plantation losses in China. Nonetheless, the information about the underlying mechanism of pathogenesis remains scarce. Therefore, in this study, we reported the role of a TCS pair CpxA/CpxR in regulating virulence and stress responses in L. populi. The CpxA/R system is essential during infection, flagellum formation, and oxidative stress response. Specifically, the Cpx system affected flagellum formation by controlling the expression of flagellum-related genes. CpxR, which was activated by phosphorylation in the presence of CpxA, participated in the transcriptional regulation of a chaperone sctU and the type III secretion system (T3SS)-related genes, thereby influencing T3SS functions during L. populi infection. Phosphorylated CpxR directly manipulated the transcription of a membrane protein-coding gene yccA and the deletion of yccA resulted in reduced virulence and increased sensitivity to H2O2. Furthermore, we mutated the conserved phosphorylation site of CpxR and found that CpxRD51A could no longer bind to the yccA promoter but could still bind to the sctU promoter. Together, our findings elucidate the roles of the Cpx system in regulating virulence and reactive oxygen species resistance and provide further evidence that the TCS is crucial during infection and stress response.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 11","pages":"e70029"},"PeriodicalIF":4.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11568244/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Abscisic acid-, stress-, ripening-induced 2 like protein, TaASR2L, promotes wheat resistance to stripe rust. 脱落酸、胁迫、成熟诱导的 2 类蛋白 TaASR2L 促进小麦对条锈病的抗性。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-11-01 DOI: 10.1111/mpp.70028

Qiao Wang, Yaqi Tang, Ying Li, Jun Ren, Hongxu Zuo, Peng Cheng, Qiang Li, Baotong Wang

{"title":"Abscisic acid-, stress-, ripening-induced 2 like protein, TaASR2L, promotes wheat resistance to stripe rust.","authors":"Qiao Wang, Yaqi Tang, Ying Li, Jun Ren, Hongxu Zuo, Peng Cheng, Qiang Li, Baotong Wang","doi":"10.1111/mpp.70028","DOIUrl":"10.1111/mpp.70028","url":null,"abstract":"Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive wheat diseases. The plant hormone abscisic acid (ABA) plays a key regulatory role in plant response to stress. ABA-, stress-, ripening-induced proteins (ASR) have been shown to be abundantly induced in response to biotic and abiotic stresses to protect plants from damage. However, the function of wheat ASR2-like protein (TaASR2L) in plants under biotic stress remains unclear. In this study, transient silencing of TaASR2L using a virus-induced gene silencing system substantially reduced wheat resistance to Pst. TaASR2L interaction with serine/arginine-rich splicing factor SR30-like (TaSR30) was validated mainly in the nucleus. Knockdown of TaSR30 expression substantially reduced wheat resistance to Pst. Overexpression of TaASR2L and TaSR30 demonstrated that they can promote the expression of ABA- and resistance-related genes to enhance wheat resistance to Pst. In addition, the expression levels of TaSR30 and TaASR2L were substantially increased by exogenous ABA, and the resistance of wheat to Pst was increased, and the expression of PR genes was induced. Therefore, these results suggest that TaASR2L interacts with TaSR30 by promoting PR genes expression and enhancing wheat resistance to Pst.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 11","pages":"e70028"},"PeriodicalIF":4.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11551255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142623901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0