Molecular plant pathology最新文献

Osmotic and pH Stress-Responsive Two-Component System, OmpR/EnvZ, Modulates Type III Secretion, Biofilm Formation, Swimming Motility and Virulence in Acidovorax citrulli xjL12. 渗透和pH胁迫响应双组分系统，OmpR/EnvZ，调节瓜酸ovorax xjL12 III型分泌、生物膜形成、游泳运动和毒力。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-06-01 DOI: 10.1111/mpp.70107

Yuanjie Wang, Chenchao Sun, Ling Cai, Shitong Wu, Wenxin Chen, Yanli Tian, Baishi Hu, Ron Walcott

{"title":"Osmotic and pH Stress-Responsive Two-Component System, OmpR/EnvZ, Modulates Type III Secretion, Biofilm Formation, Swimming Motility and Virulence in Acidovorax citrulli xjL12.","authors":"Yuanjie Wang, Chenchao Sun, Ling Cai, Shitong Wu, Wenxin Chen, Yanli Tian, Baishi Hu, Ron Walcott","doi":"10.1111/mpp.70107","DOIUrl":"10.1111/mpp.70107","url":null,"abstract":"Acidovorax citrulli, the causal pathogen of bacterial fruit blotch of cucurbits, relies on a functional type III secretion system (T3SS) for pathogenicity. Two-component systems (TCSs) are primary signal transduction mechanisms for bacteria to detect and adapt to various environmental conditions. However, the role of TCS on regulating T3SS and other virulence factors in response to environmental stimuli is still poorly understood in A. citrulli. Here, we report the identification of a conserved TCS, OmpR/EnvZ, involved in hypersensitive response (HR) induction in Nicotiana benthamiana by screening a transposon-insertion library in the group II strain xjL12 of A. citrulli. Transcription analysis confirmed that OmpRAc/EnvZAc was upregulated in response to elevated osmotic pressure, low and high pH conditions, and host environment. Deletions of envZAc, ompRAc, or both envZAc and ompRAc in A. citrulli attenuated virulence to melon seedlings and mature leaf tissues, and delayed HR in N. benthamiana. OmpRAc was activated by EnvZAc and directly bound to the promoter region of hrpG, a major regulator of T3SS. This binding activated hrpG transcription and promoted T3SS assembly in T3SS-inducing medium, XVM2. Additionally, the OmpRAc/EnvZAc mutants of A. citrulli displayed reduced swimming motility due to impaired flagella formation, but also had enhanced biofilm formation and exopolysaccharide production. OmpRAc/EnvZAc regulation of these virulence factors in A. citrulli depended on its own conserved phosphorylation sites. This work illuminates a signalling pathway for regulating the T3SS and provides insights into the OmpR/EnvZ-mediated virulence regulatory network in A. citrulli.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 6","pages":"e70107"},"PeriodicalIF":4.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alternaria solani Effector AsCEP20, Essential for Virulence, Targets Potato StFtsH4 Protein to Suppress Plant Disease Resistance. 马铃薯StFtsH4蛋白抑制植物抗病性研究进展

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-06-01 DOI: 10.1111/mpp.70109

Siyu Xiao, Jinhui Wang, Zihan Bai, Haibin Jiang, Jiehua Zhu, Zhihui Yang

{"title":"Alternaria solani Effector AsCEP20, Essential for Virulence, Targets Potato StFtsH4 Protein to Suppress Plant Disease Resistance.","authors":"Siyu Xiao, Jinhui Wang, Zihan Bai, Haibin Jiang, Jiehua Zhu, Zhihui Yang","doi":"10.1111/mpp.70109","DOIUrl":"10.1111/mpp.70109","url":null,"abstract":"Alternaria solani is an important necrotrophic pathogen causing potato early blight. However, the pathogenic molecular mechanisms of A. solani remain unclear. Previous work identified a specific effector AsCEP20 in A. solani through multi-omics analysis. AsCEP20 is required for the full virulence of A. solani and targets the host chloroplasts. In this study, we screened out 46 candidate proteins that potentially interact with AsCEP20 in Nicotiana benthamiana using co-immunoprecipitation followed by liquid chromatography-tandem mass spectrometry analysis. We identified a candidate target protein in potato, filamentation temperature-sensitive H4 (StFtsH4), which is located in chloroplasts, based on homologous alignment and subcellular localisation analysis. The interaction between AsCEP20 and StFtsH4 was further confirmed by co-immunoprecipitation, yeast two-hybrid assay and bimolecular fluorescence complementation assays. The interaction site between AsCEP20 and StFtsH4 is also the chloroplast. Silencing the potato StFtsH4 gene resulted in suppressed pathogen-associated molecular pattern-triggered reactive oxygen species (ROS) bursts, and defence-related genes were significantly downregulated. These results suggest that StFtsH4 positively regulates plant immunity. Therefore, AsCEP20 targets the chloroplast protein StFtsH4 to promote pathogen infection. AsCEP20 attenuates the efficiency of light energy utilisation in photosynthesis by targeting StFtsH4. These results suggest that AsCEP20 suppresses StFtsH4-mediated potato disease resistance to A. solani. With the increase of light intensity, ROS continued to accumulate in the chloroplast of StFtsH4-silenced plant leaves, while defence-related genes significantly decreased. Our findings reveal that the impaired StFtsH4 function limits plant photosynthesis, thereby affecting immune signalling.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 6","pages":"e70109"},"PeriodicalIF":4.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12167767/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of Pathogenicity and Evidence of Horizontal Gene Transfer in Colletotrichum gloeosporioides From a Medicinal Plant. 药用植物炭疽菌致病性丧失及水平基因转移证据。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-06-01 DOI: 10.1111/mpp.70098

Xizhen Yue, Jia Yang, Jiale Qi, Shanshan Gao, Qingmiao Huo, Xinxin Guo, Hongwei Guo, Jinmei Luo, Yiran Wang, Yirui Zhao, Rongxing Liu, He Wang, Shichen Yi, Yanping Fu, Xu Ji, Yahui Wei, Wei He, Bin Guo

{"title":"Loss of Pathogenicity and Evidence of Horizontal Gene Transfer in Colletotrichum gloeosporioides From a Medicinal Plant.","authors":"Xizhen Yue, Jia Yang, Jiale Qi, Shanshan Gao, Qingmiao Huo, Xinxin Guo, Hongwei Guo, Jinmei Luo, Yiran Wang, Yirui Zhao, Rongxing Liu, He Wang, Shichen Yi, Yanping Fu, Xu Ji, Yahui Wei, Wei He, Bin Guo","doi":"10.1111/mpp.70098","DOIUrl":"10.1111/mpp.70098","url":null,"abstract":"Colletotrichum gloeosporioides is a major agricultural pathogen of crops that has also been identified as an endophyte of the medicinal plant Huperzia serrata. Both H. serrata and C. gloeosporioides produce huperzine A, a potential treatment for Alzheimer's disease. In this study, a nonpathogenic C. gloeosporioides strain (NWUHS001) was isolated and its genome sequenced. Gene structure prediction identified 15,413 protein-coding genes and 879 noncoding RNAs. Through PHI-base database prediction, we found that NWUHS001 lacks two key pathogenicity genes CgDN3 and cap20, which may be the cause of its nonpathogenicity. Comparative genomic analysis showed that the number of genes encoding pectin lyase B (pelB), pectin lyase (pnl) and polygalacturonase (pg) in NWUHS001 was significantly lower than that in pathogenic strains during the expansion of mycelium into host tissues. This caused slow growth and incapability to penetrate host cells. In contrast, in NWUHS001, genes involved in carbon acquisition such as ribose and amino sugar metabolic pathways were enriched, indicating active metabolite exchange with the host. In addition, by comparing the genome of NWUHS001 with that of the host H. serrata, we found that polyketosynthetase (pksIII), a key gene in the host huperzine A biosynthetic pathway, may possibly have been acquired from the fungus by horizontal gene transfer (HGT). This study explained the possible genetic evolution mechanism of C. gloeosporioides from pathogenicity to nonpathogenicity, which is of value for studying the interaction between microorganisms and plants. It also provided clues to the genetic evolution of the biosynthetic pathway of huperzine A.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 6","pages":"e70098"},"PeriodicalIF":4.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12127103/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ChnagG Plays the Role of 5-Salicylate Hydroxylase in the Gentisic Acid Pathway of Salicylic Acid Metabolism in Cochliobolus heterostrophus. ChnagG在异养蜗水杨酸代谢中5-水杨酸羟化酶的作用

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-06-01 DOI: 10.1111/mpp.70090

Yadi Xu, He Wei, Haixiao Li, Fanli Zeng, Ning Liu, Zhiyan Cao, Jingao Dong

{"title":"ChnagG Plays the Role of 5-Salicylate Hydroxylase in the Gentisic Acid Pathway of Salicylic Acid Metabolism in Cochliobolus heterostrophus.","authors":"Yadi Xu, He Wei, Haixiao Li, Fanli Zeng, Ning Liu, Zhiyan Cao, Jingao Dong","doi":"10.1111/mpp.70090","DOIUrl":"10.1111/mpp.70090","url":null,"abstract":"Salicylic acid (SA) plays a crucial role in the defence strategies of plants against fungal pathogens. To circumvent plant immunity, pathogens use metabolic enzymes such as salicylate hydroxylase to degrade SA, thereby facilitating successful pathogenicity after infection. This phenomenon has not been previously reported in Cochliobolus heterostrophus. Our study demonstrates that high concentrations of SA can inhibit both growth and spore germination; however, at concentrations below 1 mM, SA does not significantly impact the growth and spore germination of C. heterostrophus, which is capable of metabolising exogenously supplied SA. Transcriptome and LC-MS analyses indicated that C. heterostrophus metabolises exogenous SA via the gentisic acid (GA) pathway, involving genes such as 5-salicylate hydroxylase (ChnagG). Prokaryotic expression of ChnagG confirmed its ability to convert SA into GA. Additionally, we created ChnagG gene deletion and complementation mutants, revealing that ChnagG influences melanin synthesis and the pathogenicity of C. heterostrophus. Analysis of the SA signalling pathway in plants during fungal infection indicated that the ChnagG knockout mutant did not alter the synthesis of SA in its host maize; however, it led to the upregulation of the downstream signalling pathway ZmPR1 gene compared to the wild type. These findings suggest that C. heterostrophus obstructs the immune signalling pathway of maize through SA metabolism, thereby enhancing its infection and pathogenicity. This study lays the groundwork for further elucidating the mechanisms underlying the interaction between maize and C. heterostrophus.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 6","pages":"e70090"},"PeriodicalIF":4.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12130555/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144209002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Aquaporin MdPIP2;2 Regulates Glomerella Leaf Spot Resistance in Apple. 水通道蛋白MdPIP2；2调控苹果肾小球叶斑病抗性。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-06-01 DOI: 10.1111/mpp.70111

Yubo Sun, Yufei Zhou, Junquan Chen, Xiaochun Ma, Xiaoqing Gong, Jie Yang, Changhai Liu, Fengwang Ma

引用次数: 0

A Lrp/AsnC Family Transcriptional Regulator Lrp Is Essential for the Pathogenicity of Dickeya oryzae. Lrp/AsnC家族转录调控因子Lrp对稻瘟病菌致病性的影响

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-06-01 DOI: 10.1111/mpp.70100

Xiaoyan Wu, Qunyi Chen, Huidi Liu, Weihan Gu, Yizhen Deng, Lian-Hui Zhang, Zhibin Liang

{"title":"A Lrp/AsnC Family Transcriptional Regulator Lrp Is Essential for the Pathogenicity of Dickeya oryzae.","authors":"Xiaoyan Wu, Qunyi Chen, Huidi Liu, Weihan Gu, Yizhen Deng, Lian-Hui Zhang, Zhibin Liang","doi":"10.1111/mpp.70100","DOIUrl":"10.1111/mpp.70100","url":null,"abstract":"Dickeya oryzae causes severe soft rot diseases in a range of important crops. To understand its complicated pathogenic mechanisms, we tried to identify the key virulence regulators through transposon mutagenesis. This led to the identification of a member of the Lrp/AsnC family transcriptional regulators in D. oryzae EC1, designated as Lrp. Phenotype analyses showed that Lrp positively regulated biofilm formation and the production of zeamines, proteases and polygalacturonases, but negatively regulated bacterial swimming motility. Deletion of lrp caused a drastic attenuation in bacterial virulence, indicating that Lrp is a key regulator in the modulation of D. oryzae pathogenicity. We further showed that the transcription of the lrp gene was negatively regulated by the transcriptional regulators SlyA, Fis and OhrR, and the transcriptional expression of tzpA, ohrR and fis was positively modulated by Lrp. Moreover, we demonstrated that Lrp can directly bind to the promoter regions of zmsA, zmsK, prtG, prtX, pehK, pehX, fis, tzpA and ohrR. DNase I footprinting assay determined that Lrp was capable of binding to a specific site (5'-GTGTAATTATGGGCGTGCTCCGGG-3') in the promoter of zmsA. Furthermore, we found that four amino acid residues of Lrp, L20, L23, G111 and T146, are essential to the biological function of Lrp. Overall, this study demonstrated that Lrp is an essential virulence modulator in D. oryzae and suggested that Lrp can be a potent target for controlling the soft rot diseases caused by D. oryzae.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 6","pages":"e70100"},"PeriodicalIF":4.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145271/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ghr-miR166b-GhHB14 Pair With GhDLO1 Partner Mediates Cotton Plant Defence Against Verticillium dahliae. Ghr-miR166b-GhHB14对GhDLO1伴侣介导棉花植株对大丽花黄萎病的防御

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-06-01 DOI: 10.1111/mpp.70104

Yuan Meng, Ge Zhao, Ye Tang, Lin Xu, Dongliang Li, Xiaoyang Ge, Jiahe Wu

{"title":"Ghr-miR166b-GhHB14 Pair With GhDLO1 Partner Mediates Cotton Plant Defence Against Verticillium dahliae.","authors":"Yuan Meng, Ge Zhao, Ye Tang, Lin Xu, Dongliang Li, Xiaoyang Ge, Jiahe Wu","doi":"10.1111/mpp.70104","DOIUrl":"10.1111/mpp.70104","url":null,"abstract":"In plants, the class III homeodomain-leucine zipper (HD-ZIP III) transcription factors regulate a broad range of developmental processes. However, less research on HD-ZIP IIIs has been done on Verticillium wilt, which is a devastating disease of cotton production worldwide. Here, we report that a cotton HD-ZIP III (GhHB14) targeted by ghr-miR166b participates in plant resistance to Verticillium wilt. According to degradome data, RACE sequencing and in vivo fluorescent light assay, ghr-miR166b can target GhHB14 mRNA to directly cleave it at nucleotide position 586, suggesting that GhHB14 expression is mediated by a post-transcriptional process. Yeast two-hybrid and bimolecular fluorescence complementation analyses showed that GhHB14 can interact with GhDLO1 in cells. Plants silenced for ghr-miR166b showed significantly higher susceptibility to Verticillium dahliae infection compared to the control, while GhHB14- and GhDLO1-silenced plants exhibited significantly higher resistance. These results suggested that ghr-miR166b is a positive regulator in plant resistance against Verticillium wilt, whereas GhHB14 and GhDLO1 are both negative regulators. Therefore, ghr-miR166b-GhHB14 coupled with GhDLO1 regulates cotton plant defence against V. dahliae.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 6","pages":"e70104"},"PeriodicalIF":4.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12151801/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144266739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systemic and Phloem-Specific Protein Targeting by High Affinity Nanobodies Expressed From a Plant RNA Virus Vector. 植物RNA病毒载体表达的高亲和力纳米体对系统和韧皮部特异性蛋白的靶向作用。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-06-01 DOI: 10.1111/mpp.70105

Angel Y S Chen, Giada Spigolon, Lorenzo Scipioni, James C K Ng

{"title":"Systemic and Phloem-Specific Protein Targeting by High Affinity Nanobodies Expressed From a Plant RNA Virus Vector.","authors":"Angel Y S Chen, Giada Spigolon, Lorenzo Scipioni, James C K Ng","doi":"10.1111/mpp.70105","DOIUrl":"10.1111/mpp.70105","url":null,"abstract":"The emergence of nanobodies (Nbs) has kindled an avid interest for their use in genetic engineering and plant biotechnology. In planta expression of Nbs has relied on either stable or transient transformation approaches that are lengthy and cannot support systemic expression, respectively. In addition, there is no precedence for studies on tissue-specific expression of Nbs. To address these issues, viral vectors could be used as an alternative, but this has not been shown. Here, this proof-of-concept study establishes a platform to demonstrate the phloem-specific targeting of proteins by Nbs expressed from a citrus tristeza virus-based vector. The vector facilitates anti-green fluorescent protein (GFP) Nb production within the phloem of transgenic Nicotiana benthamiana plants expressing a GFP-fused endoplasmic reticulum-targeting peptide and that of a microtubule marker line expressing GFP-fused α-tubulin 6. The interaction between anti-GFP Nb and the GFP-tagged peptide/protein is corroborated by both pull-down assays and fluorescence resonance energy transfer-fluorescence lifetime imaging microscopy (FRET-FLIM) measurements. This proof-of-concept platform-including validation of Nb-antigen interaction in the phloem by FRET-FLIM analysis, which has not been described in the literature-is novel for exploring Nb-mediated functions applicable to targeting or identifying phloem proteins and those co-opted into the virus infection process.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 6","pages":"e70105"},"PeriodicalIF":4.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12166124/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144294117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wheat TaNADPO Promotes Spot Blotch Resistance. 小麦TaNADPO提高抗斑点病能力。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-06-01 DOI: 10.1111/mpp.70103

Meng Yuan, Qingdong Zeng, Lei Hua, Shisheng Chen, Jianhui Wu, Shuqing Zhao, Mengyu Li, Xiaopeng Ren, Linfei Ma, Zihan Liu, Kaixuan Wang, Manli Sun, Hongfei Yan, Zhensheng Kang, Dejun Han, Xiaodong Wang

{"title":"Wheat TaNADPO Promotes Spot Blotch Resistance.","authors":"Meng Yuan, Qingdong Zeng, Lei Hua, Shisheng Chen, Jianhui Wu, Shuqing Zhao, Mengyu Li, Xiaopeng Ren, Linfei Ma, Zihan Liu, Kaixuan Wang, Manli Sun, Hongfei Yan, Zhensheng Kang, Dejun Han, Xiaodong Wang","doi":"10.1111/mpp.70103","DOIUrl":"10.1111/mpp.70103","url":null,"abstract":"Bipolaris sorokiniana is a prevalent fungal pathogen that resides in the soil and affects various parts of wheat, leading to diseases such as spot blotch, common root rot, head blight and black point. The genetic mechanisms that confer resistance in wheat against this pathogen are not completely known. In this research, 1302 wheat germplasms from around the world were evaluated for resistance to spot blotch at the seedling stage, and it was found that merely 3.8% displayed moderate or better resistance. A genome-wide association study (GWAS) employing high-density 660K single-nucleotide polymorphism (SNP) data pinpointed a segment on chromosome 1BL (621.2-674.0 Mb) containing nine SNPs that are significantly linked to spot blotch resistance, named Qsb.hebau-1BL. RNA sequencing and reverse transcription-quantitative PCR analyses demonstrated that the gene TraesCS1B02G410300, which codes for nicotinamide-adenine dinucleotide phosphate-binding oxidoreductase (TaNADPO), was markedly upregulated by B. sorokiniana. Five SNP variations were identified in the promoter region of TaNADPO in wheat lines with or without Qsb.hebau-1BL. Wheat lines that overexpressed TaNADPO exhibited increased resistance to spot blotch and higher accumulation of reactive oxygen species (ROS). In contrast, knockout EMS mutants of Triticum turgidum TdNADPO (tdnadpo-K2561, Gln125*) and TaNADPO (tanadpo-J10516796, splice donor variant) showed diminished resistance and lower ROS levels. In conclusion, TaNADPO is a key gene for resistance against B. sorokiniana, providing essential information for the development of spot blotch-resistant wheat varieties through molecular breeding techniques.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 6","pages":"e70103"},"PeriodicalIF":4.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145272/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HrpW Modulates Paracidovorax citrulli Virulence and Plant Immunity via ClRAR1 Interaction in Watermelon. HrpW通过ClRAR1互作调节瓜副酸虫病毒力和植物免疫

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-06-01 DOI: 10.1111/mpp.70108

Tong Qin, Xiuan Liang, Chen Zhong, Zhiheng Zhang, Jingjue Wang, Jiahuan Shi, Jingjing Huang, Dong Chen, Wei Zhao, Mengyang Wang, Jianlong Zhao, Yongqiang He, Shanshan Yang, Ali Chai, Xiaoxiao Zhang

{"title":"HrpW Modulates Paracidovorax citrulli Virulence and Plant Immunity via ClRAR1 Interaction in Watermelon.","authors":"Tong Qin, Xiuan Liang, Chen Zhong, Zhiheng Zhang, Jingjue Wang, Jiahuan Shi, Jingjing Huang, Dong Chen, Wei Zhao, Mengyang Wang, Jianlong Zhao, Yongqiang He, Shanshan Yang, Ali Chai, Xiaoxiao Zhang","doi":"10.1111/mpp.70108","DOIUrl":"10.1111/mpp.70108","url":null,"abstract":"Bacterial fruit blotch (BFB), caused by Paracidovorax citrulli, severely threatens watermelon production. This study investigates the role of HrpW, an atypical harpin in P. citrulli AAC00-1, in bacterial virulence and host immune modulation. Bioinformatics analysis revealed HrpW harbours a unique signal peptide and structural features distinct from other harpins. Deletion of hrpW impaired bacterial motility, biofilm formation and virulence, while complementation restored these traits. HrpW suppressed reactive oxygen species (ROS) bursts and mitogen-activated protein kinase (MAPK) activation in plants but failed to induce programmed cell death (PCD). Crucially, HrpW inhibited the hypersensitive response (HR) triggered by P. citrulli in non-host tobacco, with ΔhrpW mutant inducing premature HR. RNA-seq analysis demonstrated HrpW downregulated ClRAR1 expression. Silencing ClRAR1 or ClSGT1 compromised watermelon resistance, and notably, ClRAR1-silenced plants exhibited PCD upon HrpW treatment, indicating HrpW-mediated PCD suppression is RAR1-dependent. Importantly, HrpW triggered ubiquitin-dependent degradation of ClRAR1 and independently disrupted ClRAR1-ClSGT1 binding, thereby suppressing effector-triggered immunity (ETI). HrpW translocated into plant cells via the type III secretion system (T3SS), as confirmed by CyaA assays. Intriguingly, low concentrations of HrpW enhanced watermelon resistance to BFB, while high concentrations promoted disease progression, revealing a concentration-dependent duality. This study unveils HrpW as a multifunctional virulence factor that modulates bacterial fitness, suppresses HR and manipulates host immunity via RAR1 targeting. These findings expand our understanding of harpin-mediated pathogenicity and offer insights for sustainable BFB management strategies.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 6","pages":"e70108"},"PeriodicalIF":4.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170954/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0