Molecular plant pathology最新文献

Melatonin Enhances Panax vietnamensis Resistance to Leaf Blight Pathogen Neofusicoccum ribis via the PvWRKY40-PvCOMT2 Module-Driven Lignin Biosynthesis. 褪黑素通过PvWRKY40-PvCOMT2模块驱动木质素生物合成增强越南人参对叶枯病的抗性

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-07-01 DOI: 10.1111/mpp.70117

Mingtao Ai, Leilin Li, Jianbin Li, Jiae Hou, TianTai Liu, Zuiyi Tang, Yuan Liu, Ye Yang, Xiuming Cui, Qian Yang

{"title":"Melatonin Enhances Panax vietnamensis Resistance to Leaf Blight Pathogen Neofusicoccum ribis via the PvWRKY40-PvCOMT2 Module-Driven Lignin Biosynthesis.","authors":"Mingtao Ai, Leilin Li, Jianbin Li, Jiae Hou, TianTai Liu, Zuiyi Tang, Yuan Liu, Ye Yang, Xiuming Cui, Qian Yang","doi":"10.1111/mpp.70117","DOIUrl":"10.1111/mpp.70117","url":null,"abstract":"Panax vietnamensis, a medicinally valuable perennial herb, is highly susceptible to leaf blight under cultivation; however, the molecular mechanisms underlying this disease remain poorly understood. In this study, we identified Neofusicoccum ribis as the causal agent of P. vietnamensis leaf blight through pathogen isolation and fulfilment of Koch's postulates. Transcriptomic analysis revealed activation of phytohormone signalling (salicylic acid, jasmonic acid, and melatonin [MT]) and phenylpropanoid metabolism during infection. Among these, MT exhibited superior efficacy in inducing lignin biosynthesis compared to other hormones, with exogenous application of MT significantly enhancing lignin accumulation and improving disease resistance by 8 days post-inoculation. Further, we identified PvWRKY40 as a negative regulator of lignin synthesis, which directly binds to the W-box motif in the PvCOMT2 promoter to suppress its expression. MT counteracted this repression by downregulating PvWRKY40. Heterologous overexpression of PvCOMT2 in Nicotiana benthamiana increased lignin content and conferred enhanced resistance to Fusarium oxysporum. This study reveals a novel MT-PvWRKY40-PvCOMT2 regulatory axis governing lignin-mediated defence in P. vietnamensis, providing critical insights for combating leaf blight in cultivated ginseng.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 7","pages":"e70117"},"PeriodicalIF":4.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12227329/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CWI-MAPKs Regulate the Formation of Hyphopodia Required for Virulence in Ceratocystis fimbriata. CWI-MAPKs调控毛状角鼻虫毒力所需菌丝的形成。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-07-01 DOI: 10.1111/mpp.70119

Kailun Lu, Hao Cong, Ru Xin, Yong Sun, Qinghe Cao, Lianwei Li, Jihong Jiang

{"title":"CWI-MAPKs Regulate the Formation of Hyphopodia Required for Virulence in Ceratocystis fimbriata.","authors":"Kailun Lu, Hao Cong, Ru Xin, Yong Sun, Qinghe Cao, Lianwei Li, Jihong Jiang","doi":"10.1111/mpp.70119","DOIUrl":"10.1111/mpp.70119","url":null,"abstract":"Ceratocystis fimbriata is a destructive fungal pathogen that infects various economic crops. Nevertheless, the infection mechanism of this fungus is still unclear. Our previous studies have shown that the transcription factor CfSwi6 downstream of the cell wall integrity pathway is involved in regulating the pathogenicity of C. fimbriata. To further clarify the pathogenic mechanism of this pathway, upstream MAPKs (CfBck1-CfMkk1-CfSlt2) were characterised in this study. Deletion of CWI-MAPK genes resulted in an almost complete loss of pathogenicity of C. fimbriata. Importantly, CWI-MAPKs are associated with the formation of hyphopodia, which are infection structures required for C. fimbriata, and are reported for the first time in this work. Mutants lacking CWI-MAPK genes had defects in forming hyphopodia. The ability of mutants to penetrate cellophane membranes and host cells was reduced. CWI-MAPKs or CfSwi6 deletion affected CfSep4 assembly at penetration pegs, while CfSep4 was important for septin-ring and penetration peg formation. These results indicate that CWI-MAPKs regulate infection structure formation by modulating septin-ring organisation. RNA-seq analysis revealed that some downstream genes co-regulated by CfSlt2 and CfSwi6 are cellophane surface-induced genes. Knockout of PHH50197 and CfHSP30_1, two CfSlt2-CfSwi6-dependent genes, affected hyphopodium formation and pathogenicity. Additionally, other downstream genes, including PHH51274, CfHSP30_0, CfSTE11 and PHH55780, are not necessary for hyphopodium morphogenesis but are important for pathogenicity. Our study reveals a molecular mechanism by which CWI-MAPKs regulate pathogenicity through downstream genes mediated by CfSwi6 in C. fimbriata.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 7","pages":"e70119"},"PeriodicalIF":4.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12234376/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144584314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss-of-Function of Two PD-Associated Proteins Confers Resistance to Rice Stripe Virus. 两个pd相关蛋白的功能缺失赋予水稻条纹病毒抗性。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-07-01 DOI: 10.1111/mpp.70121

Hong Lu, Panpan Ge, Yonghuan Ma, Feng Lin, Dedao Jing, Tong Zhou, Feng Cui

{"title":"Loss-of-Function of Two PD-Associated Proteins Confers Resistance to Rice Stripe Virus.","authors":"Hong Lu, Panpan Ge, Yonghuan Ma, Feng Lin, Dedao Jing, Tong Zhou, Feng Cui","doi":"10.1111/mpp.70121","DOIUrl":"10.1111/mpp.70121","url":null,"abstract":"Plant viruses usually exploit plasmodesmata (PDs) to achieve cellular infection in host plants. Although PD-associated proteins are commonly implicated in the regulation of PD pore size, a few limited cases demonstrate their roles as viral targets suitable for resistance breeding. Here we screened the importin α protein family of rice to identify the PD-associated members and explored their effects on the infection of rice stripe virus (RSV), one of the most notorious pathogens threatening rice yields. Both Importin α1b and α4 were found to be localised on the plasma membrane and PD. Only importin α4 knockout mutant rice exhibited resistance to RSV infection while the role of importin α1b in RSV infection was negligible. The absence of importin α4 enhanced callose deposition at PDs, which impeded viral intercellular movement. Flotillin 1 is another PD-associated protein in rice and was previously reported to facilitate RSV infection. When flotillin1 and importin α4 were simultaneously knocked out, the double-knockout mutant exhibited a synergically higher resistance level to RSV not only in the greenhouse but also in natural fields without affecting agronomic traits. This study proposed the potential of the two PD-associated proteins as targets for engineering virus resistance in future.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 7","pages":"e70121"},"PeriodicalIF":4.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12227655/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Cotton Laccase Confers Disease Resistance Against Verticillium dahliae by Promoting Cell Wall Lignification. 一种棉花漆酶通过促进细胞壁木质化来抵抗大丽花黄萎病。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-07-01 DOI: 10.1111/mpp.70125

Guanfu Cheng, Chuanzong Li, Guoshuai Zhang, W G Dilantha Fernando, Yanqing Bi, Jianfeng Lei, Peihong Dai, Xiaofeng Su, Yue Li

{"title":"A Cotton Laccase Confers Disease Resistance Against Verticillium dahliae by Promoting Cell Wall Lignification.","authors":"Guanfu Cheng, Chuanzong Li, Guoshuai Zhang, W G Dilantha Fernando, Yanqing Bi, Jianfeng Lei, Peihong Dai, Xiaofeng Su, Yue Li","doi":"10.1111/mpp.70125","DOIUrl":"10.1111/mpp.70125","url":null,"abstract":"Verticillium wilt (VW), caused primarily by Verticillium dahliae, is a significant threat to cotton production. Lignification of the plant cell wall, a defence response triggered by pathogen invasion, is critical for plant resistance to numerous diseases. Laccases are known to participate in the lignification of secondary cell walls, but their role in cotton resistance to V. dahliae is not fully understood. In this study, we identified a cotton laccase gene, GhLAC14-3, that was significantly upregulated during early V. dahliae infection and was closely related to a gene previously reported to respond to V. dahliae infection in Arabidopsis. Silencing of GhLAC14-3 in cotton increased disease susceptibility and reduced lignin deposition and the expression of lignin-related genes. By contrast, overexpression of GhLAC14-3 in transgenic Arabidopsis increased lignin content and the expression of lignin-related genes, thereby enhancing VW resistance. We identified an interaction between GhLAC14-3 and the mitogen-activated protein kinase GhMAPKKK2 at the cell membrane. GhMAPKKK2 expression was also significantly induced by V. dahliae infection in cotton, and its overexpression in Arabidopsis activated multiple key resistance genes, thus improving V. dahliae resistance. Transient co-expression of GhMAPKKK2 and GhLAC14-3 in Nicotiana benthamiana leaves significantly increased lignin content. Conversely, silencing of AtMAPKKK2, the homologue of GhMAPKKK2, in GhLAC14-3-overexpressing Arabidopsis reduced both lignin levels and disease resistance. Our findings suggest that GhLAC14-3 is a promising target for enhancing VW resistance, as its interaction with GhMAPKKK2 at the cell membrane modulates defence-induced lignification.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 7","pages":"e70125"},"PeriodicalIF":4.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12257636/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The SsSmk1-SsSom1-SsMsb2 Pathway Regulates Infection Cushion Formation and Pathogenicity in Sclerotinia sclerotiorum. sssmk1 - sssom1 - sssmsb2通路调控菌核菌感染缓冲层形成和致病性

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-07-01 DOI: 10.1111/mpp.70127

Wenli Jiao, Dongmeng Ma, Hongyu Sun, Yalan Li, Xingming Lv, Qi Zuo, Sirui Liu, Hongyu Pan

{"title":"The SsSmk1-SsSom1-SsMsb2 Pathway Regulates Infection Cushion Formation and Pathogenicity in Sclerotinia sclerotiorum.","authors":"Wenli Jiao, Dongmeng Ma, Hongyu Sun, Yalan Li, Xingming Lv, Qi Zuo, Sirui Liu, Hongyu Pan","doi":"10.1111/mpp.70127","DOIUrl":"https://doi.org/10.1111/mpp.70127","url":null,"abstract":"Sclerotinia sclerotiorum causes Sclerotinia stem rot on economically important plants, posing serious threats to food security worldwide. Host-induced gene silencing (HIGS) was reported as a promising strategy for preventing infections caused by S. sclerotiorum; however, highly effective HIGS gene targets are limited. During infection, transmembrane proteins sense cell surface signals to induce infection cushion differentiation. The regulatory pathways governing intracellular signal transduction and the expression patterns of these transmembrane proteins remain unclear. Here, we demonstrated that the transcription factor SsSom1 interacted with the mitogen-activated protein kinase SsSmk1. Deletion of SsSom1 abolished sclerotia formation, regulated infection cushions development and reduced pathogenicity of S. sclerotiorum. Biochemical analysis demonstrated that SsSom1 could bind to the promoter of SsMSB2 and the SsMsb2 protein interacts with SsSte50 to activate the SsSmk1-MAPK pathway, thereby driving infection cushion differentiation of S. sclerotiorum. Furthermore, ChIP-qPCR analysis demonstrated that in the presence of SsSmk1, SsSom1 significantly enhanced the transcriptional activity of SsMSB2 under infection cushion-induced conditions. Moreover, we infiltrated HIGS constructs targeting SsSOM1 in Nicotiana benthamiana, which reduced the virulence of S. sclerotiorum. Taken together, this study elucidated the SsSmk1-SsSom1-SsMsb2 regulated infection cushions formation and the pathogenicity of S. sclerotiorum, identifying SsSom1 as a potential HIGS target for Sclerotinia stem rot control.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 7","pages":"e70127"},"PeriodicalIF":4.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144675298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytokinesis-Defective 1 (CYT1) Positively Regulates Plant Antiviral Immunity by Promoting Callose Deposition and Ascorbic Acid Biosynthesis. 细胞分裂-缺陷1 （CYT1）通过促进胼胝质沉积和抗坏血酸生物合成积极调节植物抗病毒免疫。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-07-01 DOI: 10.1111/mpp.70126

Xue Jiang, Yuting Wang, Yu Zhao, Xayvangye Korxeelor, Wenqian Fan, Xinyue Fan, Yong Li, Xiaoxia Wu, Xueping Zhou, Fangfang Li, Xiaoyun Wu, Weiqin Ji, Xiaofei Cheng

{"title":"Cytokinesis-Defective 1 (CYT1) Positively Regulates Plant Antiviral Immunity by Promoting Callose Deposition and Ascorbic Acid Biosynthesis.","authors":"Xue Jiang, Yuting Wang, Yu Zhao, Xayvangye Korxeelor, Wenqian Fan, Xinyue Fan, Yong Li, Xiaoxia Wu, Xueping Zhou, Fangfang Li, Xiaoyun Wu, Weiqin Ji, Xiaofei Cheng","doi":"10.1111/mpp.70126","DOIUrl":"10.1111/mpp.70126","url":null,"abstract":"NUCLEAR INCLUSION B (NIb), the RNA-dependent RNA polymerase (RdRp) of potyviruses, plays a critical role in both viral replication and suppression of host antiviral immunity. However, the mechanisms by which NIb suppresses host immunity remain poorly understood. In this study, we used affinity purification-mass spectrometry to identify host factors interacting with NIb encoded by turnip mosaic virus (TuMV). We identified 57 potential NIb-interacting host factors, including mannose-1-phosphate guanylyltransferase CYTOKINESIS-DEFECTIVE 1 (CYT1). Virus infectivity assays showed that TuMV infection was significantly attenuated in Nicotiana benthamiana leaves transiently expressing CYT1 and in transgenic Arabidopsis overexpressing CYT1. Exogenous application of ascorbic acid (AsA) and inhibition of N-linked glycosylation reduced virus infection. Furthermore, overexpression of CYT1 induced callose deposition, and inhibition of callose synthesis enhanced virus infection. We also demonstrated that NIb interacts with the C-terminal domain of CYT1, affects its cytosolic distribution, and inhibits AsA accumulation. These findings suggest that CYT1 positively regulates plant antiviral immunity by promoting callose deposition and ascorbic acid biosynthesis.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 7","pages":"e70126"},"PeriodicalIF":4.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12241708/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144601017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of the Transcription Factor NbTFIISL in Enhancing Bamboo Mosaic Virus Accumulation via Mitochondria Localisation. 转录因子NbTFIISL通过线粒体定位促进竹花叶病毒积累的作用

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-07-01 DOI: 10.1111/mpp.70120

Ying-Ping Huang, Jun-Wei Zhang, Ching-Ing Huang, I-Hsuan Chen, Ying-Wen Huang, Chi-Ping Cheng, Ching-Hsiu Tsai

{"title":"Role of the Transcription Factor NbTFIISL in Enhancing Bamboo Mosaic Virus Accumulation via Mitochondria Localisation.","authors":"Ying-Ping Huang, Jun-Wei Zhang, Ching-Ing Huang, I-Hsuan Chen, Ying-Wen Huang, Chi-Ping Cheng, Ching-Hsiu Tsai","doi":"10.1111/mpp.70120","DOIUrl":"10.1111/mpp.70120","url":null,"abstract":"The transcription factor NbTFIISL, a TFIIS-like protein in Nicotiana benthamiana, plays a critical role in facilitating bamboo mosaic virus (BaMV) infection. BaMV infections significantly downregulate NbTFIISL expression, with transcript levels reduced to 38% by 7 days post-inoculation. Virus-induced gene silencing of NbTFIISL impaired BaMV accumulation, reducing coat protein levels to ~45% and leading to smaller GFP-labelled infection foci. Protoplast-based assays further confirmed its involvement in viral replication, with BaMV RNA levels dropping to 12% in NbTFIISL-silenced cells. Subcellular localisation analysis revealed that NbTFIISL is primarily nuclear, directed by a classical nuclear localisation signal (NLS) and an LW motif. Mutant constructs lacking these signals-NbTFIISL/ΔNLS and NbTFIISL/ΔNLS/ΔLW-lost nuclear targeting and instead localised to mitochondria, with the double mutant forming distinct speckle-like aggregates. Overexpression experiments uncovered a dual role for NbTFIISL: nuclear-localised wild-type protein induced necrosis, whereas mitochondria-localised NbTFIISL/ΔNLS significantly enhanced BaMV accumulation (~148%). In contrast, protein aggregation in the NbTFIISL/ΔNLS/ΔLW mutant partially impaired this enhancement. Yeast two-hybrid assays revealed specific interactions between NbTFIISL and BaMV proteins, including the replicase RdRp domain and movement protein TGBp1. These findings suggest that NbTFIISL promotes BaMV replication through mitochondrial relocalisation and interaction with viral proteins, whereas its nuclear presence may trigger necrosis, potentially limiting viral spread. This study highlights the multifunctional roles of NbTFIISL and advances our understanding of host factors in plant-virus interactions and viral pathogenesis.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 7","pages":"e70120"},"PeriodicalIF":4.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12223542/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144553991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GATA8-Mediated Antiviral Defence Is Countered by Tomato Chlorosis Virus-Encoded Pathogenicity Protein p27. 番茄萎黄病毒编码致病性蛋白p27对抗gata8介导的抗病毒防御

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-07-01 DOI: 10.1111/mpp.70115

Dan Zhao, Xinghua Niu, Kaijie Shang, Guozhen Sun, Shumin Liu, Zengli Wang, Anyu Chen, Xiaoping Zhu, Lianyi Zang

{"title":"GATA8-Mediated Antiviral Defence Is Countered by Tomato Chlorosis Virus-Encoded Pathogenicity Protein p27.","authors":"Dan Zhao, Xinghua Niu, Kaijie Shang, Guozhen Sun, Shumin Liu, Zengli Wang, Anyu Chen, Xiaoping Zhu, Lianyi Zang","doi":"10.1111/mpp.70115","DOIUrl":"10.1111/mpp.70115","url":null,"abstract":"Tomato chlorosis virus (ToCV), a phloem-restricted RNA virus within the genus Crinivirus of the family Closteroviridae, exhibits a broad host range and severely impacts the yield and quality of multiple crops. Viral infection directly alters endogenous phytohormone levels, which are intricately associated with viral mobility, replication, symptom development and defence mechanisms. Previous studies have demonstrated that GATA transcription factors regulate several hormone signalling pathways in plants. In this study, we explored the interaction between ToCV p27 and SlGATA8/NbGATA11. Results indicated that ToCV p27 interacts with an 18-amino-acid at the C-terminus of SlGATA8 and NbGATA11 proteins. Silencing and overexpressing of SlGATA8 revealed its positive role in regulating tomato defence against ToCV infection. Additionally, the interaction redirected SlGATA8's subcellular localisation to plasmodesmata. Furthermore, SlGATA8 promoted the transcriptional expression of SlSnRK2 to regulate the abscisic acid (ABA) signalling pathway. In conclusion, this study confirmed that ToCV p27 impaired the transcriptional activation activity of SlGATA8 through direct interaction, thereby inhibiting the ABA pathway and ultimately facilitating viral infection. This study established a link among virus, GATA family transcription factors and phytohormones, elucidating the molecular mechanism by which ToCV-encoded p27 protein interacts with SlGATA8 to disrupt ABA balance and promote virus infection.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 7","pages":"e70115"},"PeriodicalIF":4.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12214945/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Are Fungal Disease Outbreaks Instigated by Starship Transposons? 真菌疾病爆发是由星际飞船转座子引发的吗？

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-07-01 DOI: 10.1111/mpp.70124

Andrew S Urquhart, Adrian Forsythe, Aaron A Vogan

引用次数: 0

A Sucrose-Utilisation Gene Cluster Contributes to Colonisation of Horse Chestnut by Pseudomonas syringae pv. aesculi. 一个蔗糖利用基因簇有助于丁香假单胞菌在七叶树中的定殖。aesculi。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-07-01 DOI: 10.1111/mpp.70116

Sabrine Dhaouadi, Diana Vinchira-Villarraga, Sanju Bijarniya, Amy J Webster, Federico Dorati, Carrie Brady, Dawn L Arnold, Mojgan Rabiey, Robert W Jackson

{"title":"A Sucrose-Utilisation Gene Cluster Contributes to Colonisation of Horse Chestnut by Pseudomonas syringae pv. aesculi.","authors":"Sabrine Dhaouadi, Diana Vinchira-Villarraga, Sanju Bijarniya, Amy J Webster, Federico Dorati, Carrie Brady, Dawn L Arnold, Mojgan Rabiey, Robert W Jackson","doi":"10.1111/mpp.70116","DOIUrl":"10.1111/mpp.70116","url":null,"abstract":"Pseudomonas syringae pathovar aesculi (E-Pae) causes bleeding canker disease in the woody tissue of European horse chestnut (HC). Comparative genomic analysis of E-Pae with a related leaf-infecting strain (I-Pae) and other P. syringae strains identified candidate virulence genes for colonisation of woody tissue, including a sucrose uptake and utilisation system (scrYABCDBR cluster) found in 162 of 206 P. syringae strains spanning the pangenome. Growth analysis using sucrose as sole carbon source showed that I-Pae (lacking the gene cluster) was unable to grow whereas E-Pae could grow. P. savastanoi pv. phaseolicola 1448A and P. syringae pv. morsprunorum R15244 were compromised in growth despite the presence of the gene cluster. Sucrose utilisation assays using scrB and scrY mutants and complemented strains confirmed the importance of the cluster for sucrose metabolism in vitro. Pathogenicity assays in HC revealed the sucrose gene cluster is important for symptom development in the woody tissue. While the scr genes contribute to disease causation, they were not essential for pathogen fitness when compared to hrpL and hopAB1 mutants. E-Pae caused disease symptoms in HC leaves, suggesting the strain has the potential to infect leaves as well. However, it was notable that the scrB mutant of E-Pae caused increased disease symptoms, possibly highlighting a niche adaptation strategy for I-Pae to cause leaf spots in HC as well as constraining E-Pae to predominantly infect the woody tissue.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 7","pages":"e70116"},"PeriodicalIF":4.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12227327/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0