Molecular plant pathology最新文献

The Two Tomato Ubiquitin E1 Enzymes Play Unequal Roles in Host Immunity. 两种番茄泛素E1酶在宿主免疫中的作用不同。

IF 4.9 1区农林科学

Molecular plant pathology Pub Date : 2025-10-01 DOI: 10.1111/mpp.70160

Chaofeng Wang, Bangjun Zhou, Xuanyang Chen, Lirong Zeng

{"title":"The Two Tomato Ubiquitin E1 Enzymes Play Unequal Roles in Host Immunity.","authors":"Chaofeng Wang, Bangjun Zhou, Xuanyang Chen, Lirong Zeng","doi":"10.1111/mpp.70160","DOIUrl":"10.1111/mpp.70160","url":null,"abstract":"Plants typically encode multiple ubiquitin-activating enzymes (E1s or UBAs), but their functional equivalence or divergence remains unclear. Here, we demonstrate that the two tomato (Solanum lycopersicum) E1s, SlUBA1 and SlUBA2, differentially regulate development and immunity. Knockdown of SlUBA1 or SlUBA2 caused distinct growth and developmental defects in tomato, while silencing both genes resulted in severe abnormalities, rapid etiolation, and plant death within 5-7 weeks. Notably, silencing SlUBA2, but not SlUBA1, compromised plant immunity against the bacterial pathogen Pseudomonas syringae pv. tomato (Pst). SlUBA1 and SlUBA2 exhibited distinct charging efficiencies for E2s from groups IV (SlUBC32/33/34), V (SlUBC7/14/35/36), VI (SlUBC4/5/6/15) and XII (SlUBC22), with SlUBA2 showing significantly higher efficiency. Swapping the C-terminal ubiquitin-folding domains (UFDs) between SlUBA1 and SlUBA2 largely reversed their E2-charging efficiency for these groups. Furthermore, mutating a key residue (SlUBA2Q1009) in the UFD or deleting a conserved 13-amino-acid sequence unique to group V E2s altered the E2-charging profiles of both E1s. These findings suggest dual ubiquitin-activating systems (DUAS) operate in tomato. Given the established role of group IV E2s in plant immunity against Pst, the SlUBA2-group IV E2 module likely plays a central role in modulating host defence. Similarly, the Arabidopsis E1s, AtUBA1 and AtUBA2, differentially charge homologues of tomato group IV E2s, suggesting a conserved mechanism by which plant E1s fulfil distinct physiological roles.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 10","pages":"e70160"},"PeriodicalIF":4.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12477439/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosurveillance of Invasive Southern Corn Rust: Insights Into Recent Migration Patterns and Virulence Variation. 入侵南方玉米锈病的生物监测：对近期迁移模式和毒力变异的见解。

IF 4.9 1区农林科学

Molecular plant pathology Pub Date : 2025-10-01 DOI: 10.1111/mpp.70159

Yuanjie Li, Wiruda Pootakham, Supawadee Ingsriswang, Fe Dela Cueva, Benjamine William Cordez, Yusufjion Gafforov, Jintana Unartngam, Lin Liu, Guozhi Bi, Peng Zhao, K M Tsui Clement, Junmin Liang, Lei Cai

{"title":"Biosurveillance of Invasive Southern Corn Rust: Insights Into Recent Migration Patterns and Virulence Variation.","authors":"Yuanjie Li, Wiruda Pootakham, Supawadee Ingsriswang, Fe Dela Cueva, Benjamine William Cordez, Yusufjion Gafforov, Jintana Unartngam, Lin Liu, Guozhi Bi, Peng Zhao, K M Tsui Clement, Junmin Liang, Lei Cai","doi":"10.1111/mpp.70159","DOIUrl":"10.1111/mpp.70159","url":null,"abstract":"Emerging pathogen races spreading via long-distance migration increasingly threaten global agricultural ecosystems. Understanding how pathogens migrate and adapt to new hosts via virulence evolution is crucial for developing strategies to mitigate future crop damage. Here we performed biosurveillance of Puccinia polysora, a global fungal pathogen causing southern corn rust (SCR), across China, Thailand and the Philippines. By analysing 193 field transcriptomic data, we detected both epidemic and endemic lineages co-circulating in each country and elucidated the crucial role of host selection in driving the diversification of endemic lineages. Gene flow assessments and trajectory tracking indicated that the SCR infection source in northern China is likely of domestic origin and pathogen migration from the Philippines/Thailand into China is restricted to Hainan, coastal Guangdong and southern Yunnan. We detected country-specific variants in 32 effector genes, with AvrRppC exhibiting the strongest positive selection. A phylogenetically distinct Luzon Island lineage (Philippines), carrying a novel AvrRppC allele capable of overcoming RppC-mediated resistance and represents a potentially invasive threat. Finally, we reviewed the global migration history of P. polysora in light of our findings. Our work represents the first step toward establishing an international surveillance network for P. polysora and emphasised a comprehensive control strategy integrating local governance and invasion prevention of international races.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 10","pages":"e70159"},"PeriodicalIF":4.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12483992/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145200262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High Temperature Promoted the Accumulation of Citrus Yellow Mosaic Virus in Citrus sinensis via Weakening the Immune Function of the CsWRKY76-CsPR4A Modules. 高温通过削弱CsWRKY76-CsPR4A模块的免疫功能促进柑桔黄花叶病毒在柑桔中的积累。

IF 4.9 1区农林科学

Molecular plant pathology Pub Date : 2025-10-01 DOI: 10.1111/mpp.70161

Xu-Bin Tian, Xinliang Wang, Yayu Li, Jiaxin Li, Jinhuan Zhou, Zhen Song

{"title":"High Temperature Promoted the Accumulation of Citrus Yellow Mosaic Virus in Citrus sinensis via Weakening the Immune Function of the CsWRKY76-CsPR4A Modules.","authors":"Xu-Bin Tian, Xinliang Wang, Yayu Li, Jiaxin Li, Jinhuan Zhou, Zhen Song","doi":"10.1111/mpp.70161","DOIUrl":"10.1111/mpp.70161","url":null,"abstract":"Rising global temperatures exacerbate the severity of crop diseases, threatening global agricultural production. Citrus yellow mosaic virus (CYMV) is one of the pathogens that seriously threaten citrus production, the world's largest fruit industry. However, the molecular mechanisms underlying CYMV-citrus interactions at high temperatures remain poorly understood. Over a 1-year observation period, this study found elevated temperatures increased CYMV accumulation in Madam Vinous sweet orange. Controlled experiments comparing 25°C and 37°C conditions further validated this phenomenon, with significantly higher viral titres observed under high-temperature treatments (37°C). Subsequent transcriptomic analysis revealed that the transcription factor CsWRKY76 and the pathogenesis-related gene CsPR4A were significantly downregulated in sweet orange infected with CYMV at 37°C. CsWRKY76 could directly bind to the CsPR4A promoter, thereby positively regulating CsPR4A transcription. Overexpression of CsWRKY76 or CsPR4A in transgenic citrus hairy roots significantly suppressed CYMV accumulation, while RNAi-mediated silencing of either gene promoted viral accumulation, indicating that both genes were positive regulators of citrus immunity. Overexpression of CsWRKY76 increased hydrogen peroxide content in transgenic citrus hairy roots while upregulating CsPAL2 and CsCOMT1 (involved in phenylpropanoid metabolism). This study elucidates the molecular mechanism by which high temperature suppresses the immune function of the CsWRKY76-CsPR4A modules and thereby promotes the accumulation of CYMV. Our results provide a theoretical basis for developing high-temperature-resistant disease control strategies in citrus.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 10","pages":"e70161"},"PeriodicalIF":4.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12497541/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145233069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR398-Dirigent Module Negatively Regulates Cassava Resistance to Cassava Common Mosaic Virus by Affecting the Cell Wall Lignification and Thickness.

IF 4.9 1区农林科学

Molecular plant pathology Pub Date : 2025-10-01 DOI: 10.1111/mpp.70151

Abdoulaye Assane Hamidou, Jiming Song, Yuhua Chen, Liyun Yang, Linling Zheng, Xin Chen, Yinhua Chen

{"title":"miR398-Dirigent Module Negatively Regulates Cassava Resistance to Cassava Common Mosaic Virus by Affecting the Cell Wall Lignification and Thickness.","authors":"Abdoulaye Assane Hamidou, Jiming Song, Yuhua Chen, Liyun Yang, Linling Zheng, Xin Chen, Yinhua Chen","doi":"10.1111/mpp.70151","DOIUrl":"https://doi.org/10.1111/mpp.70151","url":null,"abstract":"Studies have demonstrated microRNA398 (miR398) hyperaccumulation in plant species infected with viruses; however, its role in a viral context remains unexplored. Here, we identified cassava (Mes)-miR398 as a regulatory miRNA responsive to cassava common mosaic virus (CsCMV) infection through small-RNA analysis. Mes-miR398 targets Dirigent (Dir; Manes.05G165700.1) associated with disease resistance. Transgenic cassava lines overexpressing Mes-miR398 (OE398) showed downregulation of the Dir gene during CsCMV infection. A dual-luciferase reporter assay confirmed the direct targeting of the Dir gene by miR398. Interestingly, OE398 lines exhibited enhanced vegetative growth. However, CsCMV accumulation was significantly higher in OE398-CsCMV and Dir-silenced (SDir)-CsCMV lines and reduced in Dir-overexpressing (OEDir-CsCMV) lines, indicating a negative regulatory role of the Mes-miR398/Dir module in antiviral defence. Transmission electron microscopy revealed a reduction in cell wall thickness in CsCMV-infected plants compared with healthy controls. A similar decrease was observed in SDir lines, whereas OEDir lines maintained greater wall thickness. Furthermore, lignin content was significantly reduced in SDir lines relative to OEDir lines. Histochemical staining with Toluidine Blue O and Safranin O-Fast Green supported these findings, showing increased lignification in OEDir lines and reduced lignification in SDir lines. Subcellular localisation demonstrated that the Dir protein localises to the cell membrane, suggesting a role in regulating the cell wall membrane. These results revealed that the mes-miR398/Dir regulatory module negatively affects cassava resistance to CsCMV by altering cell wall lignification. This study is the first to identify miR398 as a regulator of a Dir gene and provides critical insights into the molecular interactions between cassava and CsCMV.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 10","pages":"e70151"},"PeriodicalIF":4.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145286615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

H₂S-Mediated GH3.1 Persulfidation Regulates IAA Homeostasis to Enhance Nodulation Formation and Nitrogen Fixation in Robinia pseudoacacia. h2s介导的GH3.1过硫化调控IAA稳态促进刺槐结瘤形成和固氮

IF 4.9 1区农林科学

Molecular plant pathology Pub Date : 2025-10-01 DOI: 10.1111/mpp.70145

Weiqin Zhang, Huaping Cheng, Xiaowu Yan, Bingyu Suo, Shiming Wen, Wuyu Liu, Gehong Wei, Juan Chen

{"title":"H2S-Mediated GH3.1 Persulfidation Regulates IAA Homeostasis to Enhance Nodulation Formation and Nitrogen Fixation in Robinia pseudoacacia.","authors":"Weiqin Zhang, Huaping Cheng, Xiaowu Yan, Bingyu Suo, Shiming Wen, Wuyu Liu, Gehong Wei, Juan Chen","doi":"10.1111/mpp.70145","DOIUrl":"10.1111/mpp.70145","url":null,"abstract":"Hydrogen sulphide (H2S), a gaseous signalling molecule, plays a multifaceted role in plant physiology by enhancing adaptability to environmental stresses. However, the regulatory mechanism of symbiotic nitrogen (N) fixation by H2S in indeterminate nodules of woody legumes remains unclear. In this study, we investigated the mechanism by which H2S promotes nodulation and N fixation in the woody legume Robinia pseudoacacia. Exogenous H2S significantly enhanced rhizobium infection, nodule formation and nitrogenase activity, demonstrating its positive role in the symbiotic process. Transcriptomic analysis of roots and nodules revealed that H2S signalling modulates auxin metabolism, particularly through the regulation of indole-3-acetic acid (IAA) homeostasis. H2S was found to promote free IAA accumulation and reduce IAA conjugation (IAA-Asp and IAA-Glu). Further investigation revealed that H2S directly targets GH3.1, a key IAA-amido synthetase responsible for IAA conjugation. Specifically, H2S mediated persulfidation at Cys304 of GH3.1, inhibiting its enzymatic activity and preventing IAA inactivation. This modification was confirmed by LC-MS/MS, UPLC-ESI-MS/MS and site-directed mutagenesis. This post-translational modification maintained active IAA levels, facilitating early nodule development. These findings highlight the active role of H2S in regulating IAA homeostasis, thereby enhancing indeterminate nodule formation and N fixation through persulfidation of the Cys304 residue of GH3.1 in R. pseudoacacia.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 10","pages":"e70145"},"PeriodicalIF":4.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12495139/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145225493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteome and Ubiquitinome Analyses Reveal the Involvement of Ubiquitination in Resistance to Maize Lethal Necrosis. 蛋白质组和泛素组分析揭示泛素化参与玉米致死性坏死的抗性。

IF 4.9 1区农林科学

Molecular plant pathology Pub Date : 2025-09-01 DOI: 10.1111/mpp.70147

Huiyan Guo, Xue Dong, Kaiqiang Hao, Xinran Gao, Jinxiu Guo, Jian Li, Shixue Zhao, Lijun Sang, Zhiping Wang, Mengnan An, Zihao Xia, Yuanhua Wu

{"title":"Proteome and Ubiquitinome Analyses Reveal the Involvement of Ubiquitination in Resistance to Maize Lethal Necrosis.","authors":"Huiyan Guo, Xue Dong, Kaiqiang Hao, Xinran Gao, Jinxiu Guo, Jian Li, Shixue Zhao, Lijun Sang, Zhiping Wang, Mengnan An, Zihao Xia, Yuanhua Wu","doi":"10.1111/mpp.70147","DOIUrl":"10.1111/mpp.70147","url":null,"abstract":"The co-infection of maize chlorotic mottle virus (MCMV) and sugarcane mosaic virus (SCMV) causes maize lethal necrosis (MLN), which seriously affects the yield and quality of maize. Ubiquitination is one of the most important protein post-translational modifications. However, the role of ubiquitination modification in regulating maize resistance to viral infection remains largely unknown. In this study, we found that the ubiquitination levels in SCMV- and/or MCMV-infected maize plants were higher than that in the non-infected maize plants. Ubiquitinome and proteome analyses of the above maize plants revealed that most down-regulated differentially accumulated proteins that possessed up-regulated lysine ubiquitination sites were mainly involved in photosynthesis, fructose and mannose metabolism, and glyoxylate and dicarboxylate metabolism. Functional analyses of three DAPs involved in glyoxylate metabolism demonstrated that silencing ZmGOX1 facilitated SCMV and MCMV single and co-infection, while knockdown of ZmHPR1 or ZmHPR2 suppressed viral infections. Moreover, overexpression of ZmGOX1 and its mutants at Kub sites enhanced maize resistance to SCMV infection. We also found that exogenous application of sodium sulphide could up-regulate the expression of ZmGOX1 and effectively inhibit viral infections. These findings provide novel insights into the roles of ubiquitination in the regulation of maize resistance to viral infection.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 9","pages":"e70147"},"PeriodicalIF":4.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12413316/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145006379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MoMad2 With a Conserved Function in the Spindle Assembly Checkpoint Is Required for Maintaining Appressorial Turgor Pressure and Pathogenicity of Rice Blast Fungus. 在纺锤体组装检查点中具有保守功能的MoMad2是维持稻瘟病菌附着体膨胀压力和致病性的必要条件。

IF 4.9 1区农林科学

Molecular plant pathology Pub Date : 2025-09-01 DOI: 10.1111/mpp.70157

Tianjiao Shen, Qiushi Chen, Ioanna Leontiou, Rong Wang, Meiling Su, Qiong Luo, Guodong Lu, Zonghua Wang, Ya Li, Kevin G Hardwick, Mo Wang

{"title":"MoMad2 With a Conserved Function in the Spindle Assembly Checkpoint Is Required for Maintaining Appressorial Turgor Pressure and Pathogenicity of Rice Blast Fungus.","authors":"Tianjiao Shen, Qiushi Chen, Ioanna Leontiou, Rong Wang, Meiling Su, Qiong Luo, Guodong Lu, Zonghua Wang, Ya Li, Kevin G Hardwick, Mo Wang","doi":"10.1111/mpp.70157","DOIUrl":"10.1111/mpp.70157","url":null,"abstract":"Mad2, a conserved core component of the spindle assembly checkpoint (SAC) in eukaryotes, delays anaphase onset in case of incorrect kinetochore-microtubule attachment. However, its functions in plant-pathogenic fungi remain largely unknown. Here, we identified the Mad2 homologue in rice blast fungus Magnaporthe oryzae (MoMad2), which shows high similarity with Mad2 in fission yeast. When expressed in fission yeast, MoMad2 associated with native SpMad1 and SpCdc20, and successfully rescued the ΔSpmad2 mutant's defect in arresting anaphase onset upon damaged spindle, indicating the conserved SAC function of MoMad2. Moreover, MoMad2 interacted with MoMad1 and depends on MoMad1 for its nuclear envelope-localisation. Although it plays a dispensable role in M. oryzae growth, MoMad2 is required for tolerance to the microtubule depolymerising agent treatment. ΔMomad2 mutants exhibited shorter hyphal compartments and earlier conidial germination and appressorium formation, suggesting that MoMad2 deletion shortens M. oryzae's mitotic cell cycle due to defective SAC arrest. Additionally, knockout of MoMAD2 decreased the appressorial turgor pressure, impaired appressorium penetration and compromised M. oryzae pathogenicity. Taken together, our findings revealed that MoMad2, as a conserved component in SAC signalling, is essential for full pathogenicity of rice blast fungus.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 9","pages":"e70157"},"PeriodicalIF":4.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12446995/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145086496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to 'Cytokinesis-Defective 1 (CYT1) Positively Regulates Plant Antiviral Immunity by Promoting Callose Deposition and Ascorbic Acid Biosynthesis'. 更正“细胞分裂-缺陷1 （CYT1）通过促进胼胝质沉积和抗坏血酸生物合成积极调节植物抗病毒免疫”。

IF 4.9 1区农林科学

Molecular plant pathology Pub Date : 2025-09-01 DOI: 10.1111/mpp.70156

引用次数: 0

The Small Cysteine-Rich Protein of Potato Mop-Top Virus Exhibits Viroporin Activity. 马铃薯拖地病毒富半胱氨酸小蛋白具有病毒蛋白活性。

IF 4.9 1区农林科学

Molecular plant pathology Pub Date : 2025-09-01 DOI: 10.1111/mpp.70150

Ye Liu, Xinyue Fan, Yameng Luan, Yong Li, Yu Zhao, Weiqin Ji, Naihui Li, Xiaoyun Wu, Xiaofei Cheng, Yanju Bai

引用次数: 0

Integrated Analysis of GC-MS-Based Metabolomics and Proteomics Reveals the Importance of Volatile Metabolite Stigmasterol in the Defence Response of Panax notoginseng Against Root Rot. 基于gc - ms的代谢组学和蛋白质组学综合分析揭示了挥发性代谢物豆甾醇在三七抗根腐病防御反应中的重要性。

IF 4.9 1区农林科学

Molecular plant pathology Pub Date : 2025-09-01 DOI: 10.1111/mpp.70144

Xiao-Min Li, Han-Lin Wang, Long-Yi Wei, Gui Li, Yuan Qu, Di-Qiu Liu

{"title":"Integrated Analysis of GC-MS-Based Metabolomics and Proteomics Reveals the Importance of Volatile Metabolite Stigmasterol in the Defence Response of Panax notoginseng Against Root Rot.","authors":"Xiao-Min Li, Han-Lin Wang, Long-Yi Wei, Gui Li, Yuan Qu, Di-Qiu Liu","doi":"10.1111/mpp.70144","DOIUrl":"10.1111/mpp.70144","url":null,"abstract":"Root rot disease in Panax notoginseng, primarily caused by the pathogenic fungus Fusarium solani, significantly impacts the growth and production of this medicinal herb. To elucidate the defence mechanisms of P. notoginseng against root rot, we employed proteomics and gas chromatography-mass spectrometry (GC-MS)-based metabolomics analyses. These analyses revealed significant accumulations of metabolites involved in phenylpropanoid, terpenoid and steroid biosynthesis pathways in F. solani-infected P. notoginseng roots. This accumulation correlated with the up-regulation of synthetases in these pathways as indicated by proteomics data. Focusing on stigmasterol, a representative steroid with differential accumulation levels, and its biosynthesis gene PnCYP710A, we investigated the role of stigmasterol metabolism in the defence response against root rot. Stigmasterol exhibited significant inhibitory effects on spore germination and hyphal growth of F. solani. Furthermore, PnCYP710A was up-regulated upon F. solani infection and induced by hormonal signals such as methyl jasmonate (MeJA). Overexpression of PnCYP710A in tobacco enhanced resistance to F. solani, up-regulated expression of JA biosynthesis/signalling pathway-related genes, increased accumulation of stigmasterol/lignin/callus, and maintained reactive oxygen species homeostasis during F. solani infection. Conversely, RNA interference (RNAi) of PnCYP710A in P. notoginseng yielded opposite effects. Additionally, PnWRKY4 positively regulated the transcription level of PnCYP710A by binding to its promoter. In summary, this study not only identifies volatile metabolites and proteins involved in the defence response of P. notoginseng against root rot but also discovers that PnWRKY4 activates stigmasterol biosynthesis to resist root rot pathogen infection.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 9","pages":"e70144"},"PeriodicalIF":4.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12405599/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0