Molecular plant pathology最新文献_第2页

GWAS With a Global Population of Wheat Stripe Rust Identified High-Confidence Avirulence Gene Candidates. 小麦条锈病全球群体的GWAS鉴定出高可信度的无毒候选基因。

IF 5 1区农林科学

Molecular plant pathology Pub Date : 2026-09-01 DOI: 10.1111/mpp.70336

Siyue Zhang, Jiaxue Peng, Zihan Fu, Yuxiang Li, Qiang Yao, Xiaoping Hu

{"title":"GWAS With a Global Population of Wheat Stripe Rust Identified High-Confidence Avirulence Gene Candidates.","authors":"Siyue Zhang, Jiaxue Peng, Zihan Fu, Yuxiang Li, Qiang Yao, Xiaoping Hu","doi":"10.1111/mpp.70336","DOIUrl":"https://doi.org/10.1111/mpp.70336","url":null,"abstract":"Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a globally destructive disease characterised by rapid virulence evolution. Despite its significance, the genetic basis of avirulence variation at a global scale remains poorly understood. In this study, we integrated virulence phenotypes against 18 Yr resistance genes with genome-wide variation from a worldwide collection of Pst isolates to resolve the population-genomic architecture of avirulence. We identified multiple avirulence-associated genomic regions and defined 69 candidate effectors, with 11 high-confidence candidates meeting five stringent criteria based on effector properties and association significance. Allele frequency analyses revealed striking geographic differentiation; many variants showed high or near-fixation in specific regions, notably East Africa, South America and inland China, while remaining rare elsewhere. This pattern indicates a strong population structure driven by regionally confined polymorphisms in host-recognition genes. Furthermore, structural modelling supported direct molecular recognition between the resistance protein Yr9 and multiple candidate AvYr9 effectors, providing mechanistic insights into specificity. Together, this study establishes a global population-genomic framework for effector discovery and elucidates virulence evolution in Pst. These candidates represent valuable resources for understanding rust pathogenicity and developing durable resistance in wheat.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"27 9","pages":"e70336"},"PeriodicalIF":5.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149030969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GmERF109 Positively Enhances Soybean Resistance to Phytophthora sojae by Transcriptionally Activating GmG4DT-Like to Promote Glyceollin I Accumulation. GmERF109通过转录激活GmG4DT-Like促进glyceolin I的积累，正向增强大豆对大豆疫霉的抗性。

IF 5 1区农林科学

Molecular plant pathology Pub Date : 2026-09-01 DOI: 10.1111/mpp.70342

Xin Fang, Rui Liang, Yiling Cheng, Hong Gao, Chuanzhong Zhang, Jingcan Sun, Junjun Song, Gengpu Zhang, Meng Liu, Yuxin Zhao, Yan Sun, Xiran Wang, Xiaoyu Chen, Kexin Ma, Shuo Wang, Kexin Li, Jiapeng Tong, Junjiang Wu, Pengfei Xu, Shuzhen Zhang

{"title":"GmERF109 Positively Enhances Soybean Resistance to Phytophthora sojae by Transcriptionally Activating GmG4DT-Like to Promote Glyceollin I Accumulation.","authors":"Xin Fang, Rui Liang, Yiling Cheng, Hong Gao, Chuanzhong Zhang, Jingcan Sun, Junjun Song, Gengpu Zhang, Meng Liu, Yuxin Zhao, Yan Sun, Xiran Wang, Xiaoyu Chen, Kexin Ma, Shuo Wang, Kexin Li, Jiapeng Tong, Junjiang Wu, Pengfei Xu, Shuzhen Zhang","doi":"10.1111/mpp.70342","DOIUrl":"10.1111/mpp.70342","url":null,"abstract":"Phytophthora root rot, a devastating disease caused by Phytophthora sojae, poses a significant threat to worldwide soybean (Glycine max) production. Therefore, enhancing crop resistance to this pathogen is a major breeding objective. However, the signalling mechanisms underlying the response of soybean plants to P. sojae infection, and the networks and targets of key transcription factors TFs, are not yet fully understood. Here, we reveal the mechanisms and function of GmERF109, which differs in expression between soybean cultivars resistant and susceptible to P. sojae race 1 and encodes an AP2/ERF transcription factor. Molecular evaluation and disease resistance analysis show that GmERF109 is a nucleus-localized transcription factor that positively regulates soybean resistance to P. sojae. We also demonstrate that GmERF109 targets and activates the expression of GmG4DT-like, a gene whose role in the biosynthesis of the phytoalexin glyceollin was confirmed through overexpression and RNA interference (RNAi) analyses. GmG4DT-like also enhances P. sojae resistance. GmG4DT-like and GmERF109 greatly increased the content of the glyceollin I isomer. Overall, our results suggest that GmERF109 enhances glyceollin accumulation by positively regulating the expression of its target gene GmG4DT-like, thereby improving soybean resistance to P. sojae. These findings provide novel insights into soybean resistance to Phytophthora root rot and will be useful in efforts to create resistant soybean cultivars.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"27 9","pages":"e70342"},"PeriodicalIF":5.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13547965/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148900843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

OsASA2 Loss-of-Function Confers Broad-Spectrum Disease Resistance and Triggers Cell Death by Regulating Anthranilate Synthase Activity in Rice. 水稻中OsASA2功能缺失通过调控邻氨基甲酸酯合成酶活性赋予广谱抗病能力并引发细胞死亡

IF 5 1区农林科学

Molecular plant pathology Pub Date : 2026-09-01 DOI: 10.1111/mpp.70340

Mingliang Guo, Shuyu Jiang, Ying Li, Zhiwei Chen, Shengping Li, Ziqiang Chen, Yuqing Niu, Mo Wang, Songbiao Chen, Dagang Tian

{"title":"OsASA2 Loss-of-Function Confers Broad-Spectrum Disease Resistance and Triggers Cell Death by Regulating Anthranilate Synthase Activity in Rice.","authors":"Mingliang Guo, Shuyu Jiang, Ying Li, Zhiwei Chen, Shengping Li, Ziqiang Chen, Yuqing Niu, Mo Wang, Songbiao Chen, Dagang Tian","doi":"10.1111/mpp.70340","DOIUrl":"10.1111/mpp.70340","url":null,"abstract":"Lesion mimic mutants are valuable for studying plant cell death and defence mechanisms. Here, we identify a rice lesion mimic mutant, osasa2-1, which exhibits spontaneous cell death and enhanced disease resistance. The mutant displays white spots on leaves that develop into large brown lesions. Histological analysis reveals increased cell death and H2O2 accumulation. Map-based cloning identified the causal gene as OsASA2 (LOC_Os03g15780). The osasa2-1 mutant carries a G496 deletion that introduces a premature stop codon, likely resulting in nonsense-mediated decay of the mutant transcript. We demonstrate that OsASA2 interacts with OsASA1, OsASB1 and OsASB2. Its loss-of-function leads to hyperactivation of anthranilate synthase (AS) activity, enhancing tryptophan biosynthesis and metabolic flux into downstream pathways. The osasa2-1 mutant exhibits elevated reactive oxygen species (ROS) levels, increased callose deposition and upregulation of defence-related genes, contributing to enhanced resistance against both the rice blast fungus Magnaporthe oryzae and the bacterial blight pathogen Xanthomonas oryzae pv. oryzae. Our findings highlight a novel regulatory role for OsASA2 in conferring broad-spectrum disease resistance by modulating AS activity and the tryptophan biosynthetic pathway in rice.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"27 9","pages":"e70340"},"PeriodicalIF":5.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13547970/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148900896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cucurbit Leaf Crumple Virus: An Important Pathogen of Cucurbit and Snap Bean Crops. 瓜叶皱缩病毒：瓜和豆荚作物的一种重要病原体。

IF 5 1区农林科学

Molecular plant pathology Pub Date : 2026-09-01 DOI: 10.1111/mpp.70345

Dalvir Kaur Dhadly, Saritha Raman Kavalappara, Sudeep Bag, Theodore McAvoy, Paul M Severns, Rajagopalbabu Srinivasan, Alvin M Simmons

{"title":"Cucurbit Leaf Crumple Virus: An Important Pathogen of Cucurbit and Snap Bean Crops.","authors":"Dalvir Kaur Dhadly, Saritha Raman Kavalappara, Sudeep Bag, Theodore McAvoy, Paul M Severns, Rajagopalbabu Srinivasan, Alvin M Simmons","doi":"10.1111/mpp.70345","DOIUrl":"10.1111/mpp.70345","url":null,"abstract":"Taxonomy: Cucurbit leaf crumple virus (CuLCrV); Begomovirus cucurbitae; Geminiviridae; Geplafuvirales.Geographical distribution: The presence of CuLCrV is exclusively limited to North America, mainly Mexico and the United States.Physical properties: CuLCrV is a bipartite begomovirus comprising two circular single-stranded DNA molecules (DNA-A and DNA-B), encapsidated within geminate icosahedral particles.Genome and organization: CuLCrV possesses a bipartite genome of DNA-A (2632 nucleotides) and DNA-B (2600 nucleotides). DNA-A contains five open reading frames (ORFs): AV1 (coat protein), AC1 (replication-associated protein), AC2 (transcriptional activator protein), AC3 (replication enhancer protein) and AC4. DNA-B contains two ORFs: BV1 (nuclear shuttle protein) and BC1 (movement protein).Transmission: CuLCrV is transmitted by the sweetpotato whitefly, Bemisia tabaci, in a persistent, circulative and non-propagative manner.Hosts: CuLCrV primarily infects crop members of the Cucurbitaceae and snap bean (Phaseolus vulgaris, Fabaceae). Multiple weed species belonging to Brassicaceae, Convolvulaceae, Cucurbitaceae and Verbenaceae act as persistent virus reservoir hosts.Symptoms: Symptom expression varies with host and infection timing. In cucurbits, infection induces leaf crumpling, thickening and downward curling of leaves, with green streaks and distortion of fruits. In snap bean, symptoms include leaf distortion, chlorosis and malformed pods.Control: No commercial cultivars with resistance to CuLCrV are available for cucurbit crops, although some resistance has been reported in snap bean cultivars. Therefore, management relies primarily on integrated disease management.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"27 9","pages":"e70345"},"PeriodicalIF":5.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13575508/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148970293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Histone Demethylase JMJD2-Dependent Regulation of rDNA Transcription and Integrity of Nucleolus Is Required for Fungal Development and Pathogenicity in the Rice Blast Fungus. 组蛋白去甲基化酶jmjd2依赖性调控rDNA转录和核仁完整性是稻瘟病真菌发育和致病性所必需的。

IF 5 1区农林科学

Molecular plant pathology Pub Date : 2026-09-01 DOI: 10.1111/mpp.70352

Song Hee Lee, Taehyeon Kim, Joo Hyun Lee, Jiyeun Park, Ki-Tae Kim, Hokyoung Son, Junhyun Jeon

{"title":"The Histone Demethylase JMJD2-Dependent Regulation of rDNA Transcription and Integrity of Nucleolus Is Required for Fungal Development and Pathogenicity in the Rice Blast Fungus.","authors":"Song Hee Lee, Taehyeon Kim, Joo Hyun Lee, Jiyeun Park, Ki-Tae Kim, Hokyoung Son, Junhyun Jeon","doi":"10.1111/mpp.70352","DOIUrl":"10.1111/mpp.70352","url":null,"abstract":"The nucleolus is a central hub for nuclear functions, including, but not limited to, ribosome biogenesis in mammals and budding yeast. However, how the nucleolus is regulated and contributes to fungal development and pathogenesis is poorly understood. Here we report that a JmjC domain-containing histone demethylase MoJMJD2 is required for rDNA transcription and nucleolar integrity in the rice blast fungus, Magnaporthe oryzae. MoJMJD2 generates two alternatively spliced isoforms with distinct subcellular localization patterns, suggesting coordinated roles in gene regulation in the nucleolus and nucleoplasm. Deletion of MoJMJD2 rendered the fungus defective in hyphal growth, sporulation and pathogenicity. Notably, loss of MoJMJD2 led to mislocalization of a nucleolar protein to the nucleoplasm and a dramatic reduction of cellular protein synthesis, suggesting the disruption of functional nucleolar structure. Taken together, these results indicate that nucleolar integrity and functions, which are under epigenetic control associated with MoJMJD2, are required for the development and pathogenicity of the rice blast fungus.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"27 9","pages":"e70352"},"PeriodicalIF":5.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13601938/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149031041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cucumber Mosaic Virus Promotes Sphingolipid Biosynthesis to Alleviate Host Unfolded Protein Response (UPR)-Mediated Immunity. 黄瓜花叶病毒促进鞘脂生物合成以减轻宿主未折叠蛋白应答（UPR）介导的免疫。

IF 5 1区农林科学

Molecular plant pathology Pub Date : 2026-09-01 DOI: 10.1111/mpp.70353

Jiayi Yao, Shaomin Wu, Shushu Gao, Zhidan Xiang, Qian Wang, Fei Ye, Qiansheng Liao, Zhouhang Gu, Zhiyou Du

{"title":"Cucumber Mosaic Virus Promotes Sphingolipid Biosynthesis to Alleviate Host Unfolded Protein Response (UPR)-Mediated Immunity.","authors":"Jiayi Yao, Shaomin Wu, Shushu Gao, Zhidan Xiang, Qian Wang, Fei Ye, Qiansheng Liao, Zhouhang Gu, Zhiyou Du","doi":"10.1111/mpp.70353","DOIUrl":"10.1111/mpp.70353","url":null,"abstract":"RNA viruses usually reprogramme host cellular lipid metabolism to create favourable replication microenvironments, yet the broader biological significance of these lipid changes is largely unknown. To explore the potential roles of virus-induced lipids, we employed an economically important plant pathogen cucumber mosaic virus (CMV), which has a single-stranded, positive-sense, tripartite RNA genome replicating in host tonoplasts. Lipidomic analysis of purified tonoplasts revealed that CMV infection increased the relative accumulation of sphingolipids in tonoplasts. This is consistent with the increased expression levels of these enzymes involved in sphingolipid biosynthesis, particularly 3-ketosphinganine reductase (TSC10). Virus-induced silencing of Nb-TSC10 in Nicotiana benthamiana impaired CMV replication by triggering the unfolded protein response (UPR). Furthermore, we found that salicylic acid (SA) serves as a central regulator of UPR-mediated antiviral immunity. Notably, a specific sphingolipid, ceramide, was found to alleviate the UPR, suggesting an important role for sphingolipids during CMV infection: CMV subverts SA-induced immunity by rewiring sphingolipid biosynthesis to inhibit the UPR-mediated defence. This work advances mechanistic understanding of viral counterdefence strategies through sphingolipid-mediated modulation of UPR during plant-pathogen interactions.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"27 9","pages":"e70353"},"PeriodicalIF":5.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13615166/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149062777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Conserved Guanyl-Specific Ribonuclease T1 Effector EC19 Is Required for Full Virulence of the Vascular Wilt Fungus Fusarium oxysporum on Tomato. 保守的鸟苷基特异性核糖核酸酶T1效应物EC19是番茄枯萎病真菌尖孢镰刀菌完全毒力的必要条件。

IF 5 1区农林科学

Molecular plant pathology Pub Date : 2026-09-01 DOI: 10.1111/mpp.70335

Min Li, Yifei Wang, Haoqian Li, Xizhe Sun, Jianbiao Wang, Xiangling Fang, Jianjun Zhao, Lisong Ma

{"title":"The Conserved Guanyl-Specific Ribonuclease T1 Effector EC19 Is Required for Full Virulence of the Vascular Wilt Fungus Fusarium oxysporum on Tomato.","authors":"Min Li, Yifei Wang, Haoqian Li, Xizhe Sun, Jianbiao Wang, Xiangling Fang, Jianjun Zhao, Lisong Ma","doi":"10.1111/mpp.70335","DOIUrl":"10.1111/mpp.70335","url":null,"abstract":"Plant fungal pathogens secrete a plethora of effectors into host cells to facilitate their infection by interfering with the normal physiological processes of the host plant. However, the role of guanyl-specific ribonuclease T1 effector protein in the virulence of fungal pathogens remains largely unexplored. Here, we show that an effector protein EC19, secreted by the soil-borne fungus Fusarium oxysporum f. sp. lycopersici (Fol) that causes tomato wilt disease, contributes to the full virulence of Fol. In-locus knockout of EC19 significantly reduced the virulence of Fol, while complementing EC19 in the knockout mutant restored the pathogenicity of Fol on tomato. EC19, which contains the ribonuclease T1-like domain, is highly conserved across fungal species and exhibited the ability to cleave single-stranded RNA isolated from tomato. EC19 was specifically induced during Fol infection. The AlphaFold3-predicted structure of EC19 shows a high degree of structural similarity with ribonuclease T1 from Aspergillus oryzae, with limited similarity to RNase T2. Using Agrobacterium-mediated transient assay in the leaves of Nicotiana benthamiana, we found that EC19 predominantly localised to the nucleus of plant cells. Stable transgenic susceptible tomato plants overexpressing EC19 exhibited increased susceptibility to Fol infection compared to wild-type plants. RNA-seq analysis of EC19-overexpressing tomato lines revealed that downregulated differentially expressed genes were predominantly enriched in plant hormone signal transduction, phenylpropanoid biosynthesis, and plant-pathogen interaction pathways. Taken together, these findings indicate that EC19 with ribonuclease activity promotes Fol infection, expanding our understanding of the molecular mechanisms underlying Fol virulence.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"27 9","pages":"e70335"},"PeriodicalIF":5.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13530400/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148865712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DrdR Negatively Modulates the Expression of Flagellar Genes via Interaction With FleQ in Xanthomonas campestris. DrdR通过与FleQ互作负向调节油菜黄单胞菌鞭毛基因的表达。

IF 5 1区农林科学

Molecular plant pathology Pub Date : 2026-09-01 DOI: 10.1111/mpp.70332

Pei-Dong Ren, Jie Zheng, Hong-Ying Sun, Yan-Hua Qi, Ji-Liang Tang, Rui-Fang Li, Guang-Tao Lu

{"title":"DrdR Negatively Modulates the Expression of Flagellar Genes via Interaction With FleQ in Xanthomonas campestris.","authors":"Pei-Dong Ren, Jie Zheng, Hong-Ying Sun, Yan-Hua Qi, Ji-Liang Tang, Rui-Fang Li, Guang-Tao Lu","doi":"10.1111/mpp.70332","DOIUrl":"10.1111/mpp.70332","url":null,"abstract":"Response regulators (RRs) of two-component signalling systems (TCSs) containing tandem receiver (REC) domains are widespread in bacteria, yet their functions and regulatory mechanisms remain poorly understood. In our previous study, DrdR, one such RR in the cruciferous black rot disease pathogen Xanthomonas campestris pv. campestris (Xcc) was demonstrated to positively regulate pilus-dependent motility and negatively regulate flagellum-dependent motility. We showed that DrdR modulates the ATPase activities of pili motor proteins PilT and PilB, thereby enhancing bacterial pilus-dependent swarming motility. However, how DrdR represses flagellar motility remained unknown. Here, we demonstrate that DrdR acts as a transcriptional repressor of flagellar gene expression. We used in vitro and in vivo approaches to identify FleQ, the master transcriptional regulator of flagellar genes, as a novel interaction partner of DrdR. Biochemical analyses revealed that DrdR binding inhibits FleQ's ATPase activity, which is essential for its transcriptional activation function. Microscale thermophoresis assays showed that DrdR reduces FleQ's DNA-binding capability to its cognate promoter. These findings collectively indicate that DrdR modulates FleQ transcriptional activity by reducing both its DNA-binding ability and ATPase activity. Our results demonstrate that DrdR serves as a specialized modulator of FleQ that acts upstream in the signalling cascade controlling the expression of flagellar genes in Xcc. This study exhibits a previously unknown mechanism whereby DrdR regulates bacterial motility. Combined with our previous finding, our data suggest that DrdR most likely acts as a conversion regulator between flagellum-dependent and pilus-dependent motility in Xcc.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"27 9","pages":"e70332"},"PeriodicalIF":5.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13535131/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148875059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The ApHSF-ApCtf1β2-ApCUT3 Cascade Couples Host ROS Detoxification to Cuticle Penetration During Fungal Pathogenesis. 在真菌发病过程中，apsf - apctf1 - β2- apcut3级联将宿主ROS解毒与角质层渗透结合起来。

IF 5 1区农林科学

Molecular plant pathology Pub Date : 2026-09-01 DOI: 10.1111/mpp.70334

Peng Yan, Lin Li, Sijia Liu, Yutong Liu, Zhanbo Liu, Han Zhao, Shujiang Li

{"title":"The ApHSF-ApCtf1β2-ApCUT3 Cascade Couples Host ROS Detoxification to Cuticle Penetration During Fungal Pathogenesis.","authors":"Peng Yan, Lin Li, Sijia Liu, Yutong Liu, Zhanbo Liu, Han Zhao, Shujiang Li","doi":"10.1111/mpp.70334","DOIUrl":"10.1111/mpp.70334","url":null,"abstract":"Successful infection by plant-pathogenic fungi requires both penetration of the host cuticle and tolerance of the reactive oxygen species (ROS) burst associated with host immunity. However, how these early infection events are temporally coordinated remains unclear. Here, we identified a three-tier transcriptional cascade, ApHSF-ApCtf1β2-ApCUT3, in Arthrinium phaeospermum, the causal agent of shoot blight in hybrid bamboo (Bambusa pervariabilis × grandis). Yeast one-hybrid, electrophoretic mobility shift and dual-luciferase assays showed that ApHSF directly binds the CTAGAA core motif in the ApCtf1β2 promoter and activates its transcription. Functional analyses further showed that ApHSF promotes detoxification of host-derived ROS by activating the fungal antioxidant system, whereas pharmacological suppression of host ROS accumulation substantially restored the pathogenicity defect of the ΔApHSF mutant. ApHSF neither bound nor independently activated the ApCUT3 promoter. Instead, yeast three-hybrid and combinatorial dual-luciferase assays showed that, under early oxidative stress, ApHSF acts as a cofactor to enhance ApCtf1β2-dependent activation of ApCUT3. In vivo fluorescence imaging further demonstrated that ROS detoxification alone was insufficient for full pathogenicity, which additionally required an intact ApCtf1β2-ApCUT3 module for cuticle penetration and colonization. Phos-tag immunoblotting, λ-protein phosphatase treatment and kinase inhibition assays showed that heat and oxidative stress induced phosphorylation of ApHSF and ApCtf1β2 and that these phosphorylation responses depended on p38 MAPK activity. By contrast, host cuticle-derived cues preferentially induced the ApCtf1β2-ApCUT3 module. Together, these findings reveal how a plant-pathogenic fungus integrates oxidative and cuticle-associated signals to coordinate early infection.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"27 9","pages":"e70334"},"PeriodicalIF":5.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13527521/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148865708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Small GTPase RHO1 Regulates ROS Homeostasis and Differentially Responds to Pathogens Infection via Interaction With Aquaporin TIP1;1 in Nicotiana. 小GTPase RHO1通过与水通道蛋白TIP1的相互作用调控ROS稳态并对病原体感染做出差异反应我在尼科提亚那。

IF 5 1区农林科学

Molecular plant pathology Pub Date : 2026-09-01 DOI: 10.1111/mpp.70333

Bowen Yuan, Chenglong Ji, Bolei Jiao, Yongchao Cheng, Zhiwei Jin, Jiale Jiang, Zeyi Xiao, Yong Liu, Jianmin Zeng, Cheng Yuan, Dehui Xi

{"title":"The Small GTPase RHO1 Regulates ROS Homeostasis and Differentially Responds to Pathogens Infection via Interaction With Aquaporin TIP1;1 in Nicotiana.","authors":"Bowen Yuan, Chenglong Ji, Bolei Jiao, Yongchao Cheng, Zhiwei Jin, Jiale Jiang, Zeyi Xiao, Yong Liu, Jianmin Zeng, Cheng Yuan, Dehui Xi","doi":"10.1111/mpp.70333","DOIUrl":"10.1111/mpp.70333","url":null,"abstract":"Small G-proteins are crucial regulators in plant growth and environmental responses. However, the mechanistic basis underlying their regulation of plant immunity to different pathogens is still poorly understood. Here, we showed that the small GTPase Ras homologous 1 (RHO1) responded differently to infection by distinct pathogens. Overexpression of RHO1 increased the plant susceptibility to chilli veinal mottle virus (ChiVMV) and necrotrophic Botrytis cinerea infections but enhanced plant resistance to the biotrophic bacterium Pseudomonas syringae. Further exploration revealed that there existed a direct interaction between RHO1 and tonoplast intrinsic protein 1;1 (TIP1;1), an aquaporin involved in water movement and the transport of hydrogen peroxide (H2O2) between vacuoles and cytoplasm. Under pathogen infection, the interaction between RHO1 and TIP1;1 could affect the distribution of reactive oxygen species (ROS) in the cytoplasm and vacuoles, promote the accumulation of ROS in the cytoplasm, leading to different responses of plant cells to pathogens with different lifestyles. Furthermore, the expression level of TIP1;1 was altered under infection by different pathogens. Our results demonstrate a mechanism by which the small GTPase RHO1 regulates ROS compartmentalization to affect the plant response to different pathogen infections, indicating the diversity of responses of a plant gene to different biotic factors.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"27 9","pages":"e70333"},"PeriodicalIF":5.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13525207/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148850946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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