Molecular plant pathology最新文献_第2页

Are Fungal Disease Outbreaks Instigated by Starship Transposons? 真菌疾病爆发是由星际飞船转座子引发的吗？

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-07-01 DOI: 10.1111/mpp.70124

Andrew S Urquhart, Adrian Forsythe, Aaron A Vogan

引用次数: 0

A Sucrose-Utilisation Gene Cluster Contributes to Colonisation of Horse Chestnut by Pseudomonas syringae pv. aesculi. 一个蔗糖利用基因簇有助于丁香假单胞菌在七叶树中的定殖。aesculi。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-07-01 DOI: 10.1111/mpp.70116

Sabrine Dhaouadi, Diana Vinchira-Villarraga, Sanju Bijarniya, Amy J Webster, Federico Dorati, Carrie Brady, Dawn L Arnold, Mojgan Rabiey, Robert W Jackson

{"title":"A Sucrose-Utilisation Gene Cluster Contributes to Colonisation of Horse Chestnut by Pseudomonas syringae pv. aesculi.","authors":"Sabrine Dhaouadi, Diana Vinchira-Villarraga, Sanju Bijarniya, Amy J Webster, Federico Dorati, Carrie Brady, Dawn L Arnold, Mojgan Rabiey, Robert W Jackson","doi":"10.1111/mpp.70116","DOIUrl":"10.1111/mpp.70116","url":null,"abstract":"Pseudomonas syringae pathovar aesculi (E-Pae) causes bleeding canker disease in the woody tissue of European horse chestnut (HC). Comparative genomic analysis of E-Pae with a related leaf-infecting strain (I-Pae) and other P. syringae strains identified candidate virulence genes for colonisation of woody tissue, including a sucrose uptake and utilisation system (scrYABCDBR cluster) found in 162 of 206 P. syringae strains spanning the pangenome. Growth analysis using sucrose as sole carbon source showed that I-Pae (lacking the gene cluster) was unable to grow whereas E-Pae could grow. P. savastanoi pv. phaseolicola 1448A and P. syringae pv. morsprunorum R15244 were compromised in growth despite the presence of the gene cluster. Sucrose utilisation assays using scrB and scrY mutants and complemented strains confirmed the importance of the cluster for sucrose metabolism in vitro. Pathogenicity assays in HC revealed the sucrose gene cluster is important for symptom development in the woody tissue. While the scr genes contribute to disease causation, they were not essential for pathogen fitness when compared to hrpL and hopAB1 mutants. E-Pae caused disease symptoms in HC leaves, suggesting the strain has the potential to infect leaves as well. However, it was notable that the scrB mutant of E-Pae caused increased disease symptoms, possibly highlighting a niche adaptation strategy for I-Pae to cause leaf spots in HC as well as constraining E-Pae to predominantly infect the woody tissue.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 7","pages":"e70116"},"PeriodicalIF":4.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12227327/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

E3-Ubiquitin Ligase SgATL31 Promotes Anthracnose Resistance in Stylosanthes by Modulating ROS Burst and Antioxidant Defence: A Proteomic and Functional Study. e3 -泛素连接酶sgart31通过调节ROS爆发和抗氧化防御促进柱花草的炭疽病抗性：蛋白质组学和功能研究

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-07-01 DOI: 10.1111/mpp.70122

Liyun Yang, Yunpiao Long, Mengze Gao, Shizi Zhang, Jing Gao, Lijuan Luo, Lingyan Jiang

{"title":"E3-Ubiquitin Ligase SgATL31 Promotes Anthracnose Resistance in Stylosanthes by Modulating ROS Burst and Antioxidant Defence: A Proteomic and Functional Study.","authors":"Liyun Yang, Yunpiao Long, Mengze Gao, Shizi Zhang, Jing Gao, Lijuan Luo, Lingyan Jiang","doi":"10.1111/mpp.70122","DOIUrl":"10.1111/mpp.70122","url":null,"abstract":"Stylosanthes spp. (stylo) is an important leguminous forage cultivated in tropical areas. Anthracnose caused by Colletotrichum gloeosporioides is a destructive disease that limits the yield of stylo. Therefore, improving the resistance of stylo is crucial to control stylo anthracnose. In this study, the resistance evaluation of 40 Chinese stylo accessions was performed, including the main cultivar Stylosanthes guianensis 'Reyan No. 2' (RY2) as a susceptible control. Twelve stylo accessions were rated as highly resistant, with 2001-84 showing the strongest resistance. Compared to RY2, 2001-84 exhibited significantly milder disease symptoms, slower fungal colonisation, and higher pathogen-induced antioxidant enzyme activities. Integrated phosphoproteomics and plasma membrane (PM) enriched proteomics of both RY2 and 2001-84 revealed that pathogen-responsive proteins were predominantly associated with kinase signalling, transport processes, and oxidoreductase activity. A PM-localised E3 ubiquitin ligase, SgATL31, was identified as increasing in response to pathogen in both proteomic analyses. Functional characterisation demonstrated that SgATL31 overexpression in Arabidopsis enhanced resistance to C. gloeosporioides, promoted chitin-induced reactive oxygen species (ROS) production in both Arabidopsis and stylo protoplasts, and increased antioxidant enzyme activities following pathogen infection. Furthermore, the expression levels of SgATL31 were induced by pathogen infection in all 40 stylo accessions and accumulated to higher levels in resistant accessions. Overall, our findings not only identify 2001-84 as a valuable genetic resource for anthracnose resistance but also establish SgATL31 as a regulator of plant immunity against anthracnose, potentially through modulation of ROS and antioxidant pathways, providing important insights for improving disease resistance in stylo.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 7","pages":"e70122"},"PeriodicalIF":4.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12234378/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144584315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

N-Glycosylation-The Behind-the-Scenes 'Manipulative Hand' of Plant Pathogen Invasiveness. n-糖基化-植物病原体入侵背后的“操纵之手”

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-07-01 DOI: 10.1111/mpp.70123

Hao Jiang, Yamin Du, Maorun Fu, Wenxiao Jiao

{"title":"N-Glycosylation-The Behind-the-Scenes 'Manipulative Hand' of Plant Pathogen Invasiveness.","authors":"Hao Jiang, Yamin Du, Maorun Fu, Wenxiao Jiao","doi":"10.1111/mpp.70123","DOIUrl":"10.1111/mpp.70123","url":null,"abstract":"Plant pathogens infect hosts through sophisticated molecular strategies, with N-glycosylation serving as a critical post-translational modification that regulates their virulence. This review comprehensively summarises and discusses the role of N-glycosylation in regulating potential pathogenic mechanisms, including fungal growth and development, cell wall integrity, infection structures (e.g., appressoria, invasive hyphae) and the secretion of effector proteins. By ensuring proper protein folding, stability and interaction with host defences, N-glycosylation enables pathogens to evade plant immune recognition, such as pathogen-associated molecular pattern (PAMP)-triggered immunity, and establish successful infections. Advances in glycomic techniques and proteomics offer tools to dissect these mechanisms with enhanced precision. Future research directions regarding N-glycosylation in plant pathogens should emphasise the development of small-molecule targeted drugs against pathogens and the creation of enzyme inhibitors to disrupt virulence factors. This work provides a perspective for further study regarding N-glycosylation in the plant-pathogenic mechanisms and effective disease control.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 7","pages":"e70123"},"PeriodicalIF":4.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12234393/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144584316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Enzyme Glucose-1-Phosphate Thymidylyltransferase RmlA Plays a Crucial Role in the Pathogenesis of Pectobacterium actinidiae GX1. 葡萄糖-1-磷酸胸苷基转移酶RmlA在果胶杆菌GX1的发病机制中起着至关重要的作用。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-07-01 DOI: 10.1111/mpp.70118

Zhixiang Yuan, Yijie Yu, Tingmi Yang, Yuwei Xue, Jiangfei Hu, Njoroge Hellen Wambui, Zhuang Liu, Mingzhao Wang, Hongxia Liu

{"title":"The Enzyme Glucose-1-Phosphate Thymidylyltransferase RmlA Plays a Crucial Role in the Pathogenesis of Pectobacterium actinidiae GX1.","authors":"Zhixiang Yuan, Yijie Yu, Tingmi Yang, Yuwei Xue, Jiangfei Hu, Njoroge Hellen Wambui, Zhuang Liu, Mingzhao Wang, Hongxia Liu","doi":"10.1111/mpp.70118","DOIUrl":"10.1111/mpp.70118","url":null,"abstract":"Pectobacterium actinidiae is one of the primary pathogens that causes summer canker disease in kiwifruit, yet its pathogenic mechanisms remain unknown. The exopolysaccharide PCAP-1a, isolated from the fermentation broth of P. actinidiae strain GX1, exhibits notable cytotoxicity and acts as a virulence factor facilitating host infection. Genome-wide analysis revealed a 21-gene cluster responsible for the biosynthesis of exopolysaccharides in GX1. Homologous recombination was used to systematically knock out these genes, which led to the identification of RmlA as a key protein in the synthesis of the PCAP-1a precursor. The deletion of the rmlA gene significantly affected the yield of PCAP-1a and resulted in a direct reduction in GX1 pathogenicity. Further studies revealed that mutations in the substrate binding site of RmlA weakened its capacity to bind G-1-P and dTTP, which led to markedly reduced pathogenicity in the corresponding complemented strains. This study indicates that the exopolysaccharide PCAP-1a serves as a virulence factor in the pathogenesis of GX1, and its biosynthesis depends on the polysaccharide synthesis gene rmlA and the substrate binding activity of its encoded protein.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 7","pages":"e70118"},"PeriodicalIF":4.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12227328/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Osmotic and pH Stress-Responsive Two-Component System, OmpR/EnvZ, Modulates Type III Secretion, Biofilm Formation, Swimming Motility and Virulence in Acidovorax citrulli xjL12. 渗透和pH胁迫响应双组分系统，OmpR/EnvZ，调节瓜酸ovorax xjL12 III型分泌、生物膜形成、游泳运动和毒力。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-06-01 DOI: 10.1111/mpp.70107

Yuanjie Wang, Chenchao Sun, Ling Cai, Shitong Wu, Wenxin Chen, Yanli Tian, Baishi Hu, Ron Walcott

{"title":"Osmotic and pH Stress-Responsive Two-Component System, OmpR/EnvZ, Modulates Type III Secretion, Biofilm Formation, Swimming Motility and Virulence in Acidovorax citrulli xjL12.","authors":"Yuanjie Wang, Chenchao Sun, Ling Cai, Shitong Wu, Wenxin Chen, Yanli Tian, Baishi Hu, Ron Walcott","doi":"10.1111/mpp.70107","DOIUrl":"10.1111/mpp.70107","url":null,"abstract":"Acidovorax citrulli, the causal pathogen of bacterial fruit blotch of cucurbits, relies on a functional type III secretion system (T3SS) for pathogenicity. Two-component systems (TCSs) are primary signal transduction mechanisms for bacteria to detect and adapt to various environmental conditions. However, the role of TCS on regulating T3SS and other virulence factors in response to environmental stimuli is still poorly understood in A. citrulli. Here, we report the identification of a conserved TCS, OmpR/EnvZ, involved in hypersensitive response (HR) induction in Nicotiana benthamiana by screening a transposon-insertion library in the group II strain xjL12 of A. citrulli. Transcription analysis confirmed that OmpRAc/EnvZAc was upregulated in response to elevated osmotic pressure, low and high pH conditions, and host environment. Deletions of envZAc, ompRAc, or both envZAc and ompRAc in A. citrulli attenuated virulence to melon seedlings and mature leaf tissues, and delayed HR in N. benthamiana. OmpRAc was activated by EnvZAc and directly bound to the promoter region of hrpG, a major regulator of T3SS. This binding activated hrpG transcription and promoted T3SS assembly in T3SS-inducing medium, XVM2. Additionally, the OmpRAc/EnvZAc mutants of A. citrulli displayed reduced swimming motility due to impaired flagella formation, but also had enhanced biofilm formation and exopolysaccharide production. OmpRAc/EnvZAc regulation of these virulence factors in A. citrulli depended on its own conserved phosphorylation sites. This work illuminates a signalling pathway for regulating the T3SS and provides insights into the OmpR/EnvZ-mediated virulence regulatory network in A. citrulli.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 6","pages":"e70107"},"PeriodicalIF":4.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tomato Spotted Wilt Virus Promotes Offspring and Egg Production of Its Vector, Frankliniella occidentalis, by Suppressing Plant Defences Induced by a Thrips Salivary Elicitor. 番茄斑点枯萎病毒通过抑制蓟马唾液激发子诱导的植物防御来促进其载体西富兰克林菌的后代和产卵。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-06-01 DOI: 10.1111/mpp.70112

Lingna Shangguan, Ronzhen Chen, Yu Zhang, Shujuan Ye, Chentao Hong, Hongmin Cui, Haohua Yu, Chongkun Zuo, Junheng Pan, Jiayi Hu, Mingfeng Feng, Jia Li, Xiaorong Tao, Min Zhu

{"title":"Tomato Spotted Wilt Virus Promotes Offspring and Egg Production of Its Vector, Frankliniella occidentalis, by Suppressing Plant Defences Induced by a Thrips Salivary Elicitor.","authors":"Lingna Shangguan, Ronzhen Chen, Yu Zhang, Shujuan Ye, Chentao Hong, Hongmin Cui, Haohua Yu, Chongkun Zuo, Junheng Pan, Jiayi Hu, Mingfeng Feng, Jia Li, Xiaorong Tao, Min Zhu","doi":"10.1111/mpp.70112","DOIUrl":"10.1111/mpp.70112","url":null,"abstract":"Western flower thrips (Frankliniella occidentalis) are among the most significant invasive pests worldwide. In addition to causing direct plant damage, they transmit tomato spotted wilt virus (TSWV) (species Orthotospovirus tomatomaculae; genus Orthotospovirus), a member of the genus Orthotospovirus. Although numerous studies have examined virus-insect-host plant interactions, research on the TSWV-thrips-plant tripartite interaction remains limited. In this study, we found that F. occidentalis can induce plant defence responses. FoCSP1, a chemosensory protein from F. occidentalis, was identified as a salivary elicitor capable of inducing serial plant defence responses in Nicotiana benthamiana. Our results revealed that the FoCSP1-induced plant defence responses did not affect thrips feeding preference but significantly inhibited both offspring and egg production. Moreover, TSWV impairs these defence responses through its encoded proteins, N and NSs, thereby alleviating the FoCSP1-mediated suppression of thrips offspring and egg production. Collectively, these findings indicate that TSWV promotes the offspring and egg production of its thrips vector by inhibiting plant defences induced by FoCSP1, providing new insights into the TSWV-thrips-plant tripartite interaction.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 6","pages":"e70112"},"PeriodicalIF":4.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186865/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alternaria solani Effector AsCEP20, Essential for Virulence, Targets Potato StFtsH4 Protein to Suppress Plant Disease Resistance. 马铃薯StFtsH4蛋白抑制植物抗病性研究进展

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-06-01 DOI: 10.1111/mpp.70109

Siyu Xiao, Jinhui Wang, Zihan Bai, Haibin Jiang, Jiehua Zhu, Zhihui Yang

{"title":"Alternaria solani Effector AsCEP20, Essential for Virulence, Targets Potato StFtsH4 Protein to Suppress Plant Disease Resistance.","authors":"Siyu Xiao, Jinhui Wang, Zihan Bai, Haibin Jiang, Jiehua Zhu, Zhihui Yang","doi":"10.1111/mpp.70109","DOIUrl":"10.1111/mpp.70109","url":null,"abstract":"Alternaria solani is an important necrotrophic pathogen causing potato early blight. However, the pathogenic molecular mechanisms of A. solani remain unclear. Previous work identified a specific effector AsCEP20 in A. solani through multi-omics analysis. AsCEP20 is required for the full virulence of A. solani and targets the host chloroplasts. In this study, we screened out 46 candidate proteins that potentially interact with AsCEP20 in Nicotiana benthamiana using co-immunoprecipitation followed by liquid chromatography-tandem mass spectrometry analysis. We identified a candidate target protein in potato, filamentation temperature-sensitive H4 (StFtsH4), which is located in chloroplasts, based on homologous alignment and subcellular localisation analysis. The interaction between AsCEP20 and StFtsH4 was further confirmed by co-immunoprecipitation, yeast two-hybrid assay and bimolecular fluorescence complementation assays. The interaction site between AsCEP20 and StFtsH4 is also the chloroplast. Silencing the potato StFtsH4 gene resulted in suppressed pathogen-associated molecular pattern-triggered reactive oxygen species (ROS) bursts, and defence-related genes were significantly downregulated. These results suggest that StFtsH4 positively regulates plant immunity. Therefore, AsCEP20 targets the chloroplast protein StFtsH4 to promote pathogen infection. AsCEP20 attenuates the efficiency of light energy utilisation in photosynthesis by targeting StFtsH4. These results suggest that AsCEP20 suppresses StFtsH4-mediated potato disease resistance to A. solani. With the increase of light intensity, ROS continued to accumulate in the chloroplast of StFtsH4-silenced plant leaves, while defence-related genes significantly decreased. Our findings reveal that the impaired StFtsH4 function limits plant photosynthesis, thereby affecting immune signalling.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 6","pages":"e70109"},"PeriodicalIF":4.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12167767/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ChnagG Plays the Role of 5-Salicylate Hydroxylase in the Gentisic Acid Pathway of Salicylic Acid Metabolism in Cochliobolus heterostrophus. ChnagG在异养蜗水杨酸代谢中5-水杨酸羟化酶的作用

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-06-01 DOI: 10.1111/mpp.70090

Yadi Xu, He Wei, Haixiao Li, Fanli Zeng, Ning Liu, Zhiyan Cao, Jingao Dong

{"title":"ChnagG Plays the Role of 5-Salicylate Hydroxylase in the Gentisic Acid Pathway of Salicylic Acid Metabolism in Cochliobolus heterostrophus.","authors":"Yadi Xu, He Wei, Haixiao Li, Fanli Zeng, Ning Liu, Zhiyan Cao, Jingao Dong","doi":"10.1111/mpp.70090","DOIUrl":"10.1111/mpp.70090","url":null,"abstract":"Salicylic acid (SA) plays a crucial role in the defence strategies of plants against fungal pathogens. To circumvent plant immunity, pathogens use metabolic enzymes such as salicylate hydroxylase to degrade SA, thereby facilitating successful pathogenicity after infection. This phenomenon has not been previously reported in Cochliobolus heterostrophus. Our study demonstrates that high concentrations of SA can inhibit both growth and spore germination; however, at concentrations below 1 mM, SA does not significantly impact the growth and spore germination of C. heterostrophus, which is capable of metabolising exogenously supplied SA. Transcriptome and LC-MS analyses indicated that C. heterostrophus metabolises exogenous SA via the gentisic acid (GA) pathway, involving genes such as 5-salicylate hydroxylase (ChnagG). Prokaryotic expression of ChnagG confirmed its ability to convert SA into GA. Additionally, we created ChnagG gene deletion and complementation mutants, revealing that ChnagG influences melanin synthesis and the pathogenicity of C. heterostrophus. Analysis of the SA signalling pathway in plants during fungal infection indicated that the ChnagG knockout mutant did not alter the synthesis of SA in its host maize; however, it led to the upregulation of the downstream signalling pathway ZmPR1 gene compared to the wild type. These findings suggest that C. heterostrophus obstructs the immune signalling pathway of maize through SA metabolism, thereby enhancing its infection and pathogenicity. This study lays the groundwork for further elucidating the mechanisms underlying the interaction between maize and C. heterostrophus.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 6","pages":"e70090"},"PeriodicalIF":4.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12130555/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144209002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of Pathogenicity and Evidence of Horizontal Gene Transfer in Colletotrichum gloeosporioides From a Medicinal Plant. 药用植物炭疽菌致病性丧失及水平基因转移证据。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-06-01 DOI: 10.1111/mpp.70098

Xizhen Yue, Jia Yang, Jiale Qi, Shanshan Gao, Qingmiao Huo, Xinxin Guo, Hongwei Guo, Jinmei Luo, Yiran Wang, Yirui Zhao, Rongxing Liu, He Wang, Shichen Yi, Yanping Fu, Xu Ji, Yahui Wei, Wei He, Bin Guo

{"title":"Loss of Pathogenicity and Evidence of Horizontal Gene Transfer in Colletotrichum gloeosporioides From a Medicinal Plant.","authors":"Xizhen Yue, Jia Yang, Jiale Qi, Shanshan Gao, Qingmiao Huo, Xinxin Guo, Hongwei Guo, Jinmei Luo, Yiran Wang, Yirui Zhao, Rongxing Liu, He Wang, Shichen Yi, Yanping Fu, Xu Ji, Yahui Wei, Wei He, Bin Guo","doi":"10.1111/mpp.70098","DOIUrl":"10.1111/mpp.70098","url":null,"abstract":"Colletotrichum gloeosporioides is a major agricultural pathogen of crops that has also been identified as an endophyte of the medicinal plant Huperzia serrata. Both H. serrata and C. gloeosporioides produce huperzine A, a potential treatment for Alzheimer's disease. In this study, a nonpathogenic C. gloeosporioides strain (NWUHS001) was isolated and its genome sequenced. Gene structure prediction identified 15,413 protein-coding genes and 879 noncoding RNAs. Through PHI-base database prediction, we found that NWUHS001 lacks two key pathogenicity genes CgDN3 and cap20, which may be the cause of its nonpathogenicity. Comparative genomic analysis showed that the number of genes encoding pectin lyase B (pelB), pectin lyase (pnl) and polygalacturonase (pg) in NWUHS001 was significantly lower than that in pathogenic strains during the expansion of mycelium into host tissues. This caused slow growth and incapability to penetrate host cells. In contrast, in NWUHS001, genes involved in carbon acquisition such as ribose and amino sugar metabolic pathways were enriched, indicating active metabolite exchange with the host. In addition, by comparing the genome of NWUHS001 with that of the host H. serrata, we found that polyketosynthetase (pksIII), a key gene in the host huperzine A biosynthetic pathway, may possibly have been acquired from the fungus by horizontal gene transfer (HGT). This study explained the possible genetic evolution mechanism of C. gloeosporioides from pathogenicity to nonpathogenicity, which is of value for studying the interaction between microorganisms and plants. It also provided clues to the genetic evolution of the biosynthetic pathway of huperzine A.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 6","pages":"e70098"},"PeriodicalIF":4.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12127103/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0