Molecular plant pathology最新文献_第2页

Regulation of Oomycete Autophagy, Lipid Droplet Accumulation and Pathogenesis by Three Rab GTPases. 三种兔gtpase对卵菌自噬、脂滴积累及发病机制的调控。

IF 4.9 1区农林科学

Molecular plant pathology Pub Date : 2025-09-01 DOI: 10.1111/mpp.70154

Gangqiang Zhou, Honglin Chen, Wenzhe Zhou, Rubin Shen, Lizhu Xie, Gao Gao, Junjian Situ, Pinggen Xi, Minhui Li, Wen Li, Tom Hsiang, Zide Jiang, Guanghui Kong

{"title":"Regulation of Oomycete Autophagy, Lipid Droplet Accumulation and Pathogenesis by Three Rab GTPases.","authors":"Gangqiang Zhou, Honglin Chen, Wenzhe Zhou, Rubin Shen, Lizhu Xie, Gao Gao, Junjian Situ, Pinggen Xi, Minhui Li, Wen Li, Tom Hsiang, Zide Jiang, Guanghui Kong","doi":"10.1111/mpp.70154","DOIUrl":"10.1111/mpp.70154","url":null,"abstract":"Among eukaryotes, Rab GTPases are critical for intracellular membrane trafficking and possess various functions. Oomycetes, responsible for many devastating plant diseases, pose a significant threat to global agriculture. However, the functions of Rab GTPases in oomycetes are largely uncharted. In this study, we functionally characterised two Rab8 homologues, PlRab8A and PlRab8B, from a plant-pathogenic oomycete, Peronophythora litchii. These genes are crucial for mycelial growth and play significant roles in the production and morphology of sporangia, as well as the formation of zoospores. PlRab8A and PlRab8B also contribute to maintaining cell wall integrity, osmotic stress tolerance and oospore formation, which are vital for the organism's survival and virulence. Moreover, PlRab8A and PlRab8B are essential for the full virulence of P. litchii by influencing laccase activity. We also found that PlRab8B co-localised with lipid droplets (LDs) and negatively affected LD accumulation. Autophagy was enhanced and autophagy-related genes (PlATGs) were up-regulated in PlRab8B mutants. Additionally, PlRab8A was found to interact with PlRab6. Similar to PlRab8, PlRab6 is also required for growth, zoospore formation and pathogenicity of P. litchii. Taken together, this study reported for the first time the functions of three Rab proteins regulating autophagy, LD accumulation and pathogenesis in oomycetes.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 9","pages":"e70154"},"PeriodicalIF":4.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12420355/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145030297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of the Matrix Protein as a Conserved and Central Determinant of Superinfection Exclusion in Plant Rhabdoviruses. 基质蛋白作为植物横纹肌病毒排除重复感染的保守和中心决定因素的鉴定。

IF 4.9 1区农林科学

Molecular plant pathology Pub Date : 2025-09-01 DOI: 10.1111/mpp.70152

Junyun Jiang, Shuang Ni, Shuo Wang, Li Xie, Zhenghe Li

{"title":"Identification of the Matrix Protein as a Conserved and Central Determinant of Superinfection Exclusion in Plant Rhabdoviruses.","authors":"Junyun Jiang, Shuang Ni, Shuo Wang, Li Xie, Zhenghe Li","doi":"10.1111/mpp.70152","DOIUrl":"10.1111/mpp.70152","url":null,"abstract":"Superinfection exclusion (SIE) is a finely tuned virus-virus interaction mechanism closely linked to the viral infection cycle. However, the mechanistic basis of SIE remains incompletely understood in plant viruses, particularly among negative-sense, single-stranded RNA viruses. In this study, we first describe the development of an efficient reverse genetics system for the plant nucleorhabdovirus Physostegia chlorotic mottle virus (PhCMoV) by codon optimisation of the large polymerase coding sequence. Using fluorescently tagged variants of PhCMoV, as well as three additional closely or distantly related plant rhabdoviruses, we found that each rhabdovirus displayed homotypic SIE. Moreover, two closely related alphanucleorhabdoviruses, PhCMoV and eggplant mottled dwarf virus, also exhibited mutual exclusion. Loss- and gain-of-function reverse genetics analyses identified the rhabdovirus matrix (M) protein as the central SIE effector: M-deficient mutant viruses lost exclusion capacity, whereas ectopically expressed heterologous M proteins conferred SIE against otherwise compatible, distantly related rhabdoviruses. Additional functional assays demonstrated that the ability of rhabdovirus M proteins to suppress cognate and noncognate viral RNA synthesis correlated with the intra- and interspecies SIE capacity. The widespread occurrence of SIE across distinct plant rhabdoviruses underscores its importance for understanding the viral replication cycle and highlights its practical relevance for the development of novel virus control strategies.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 9","pages":"e70152"},"PeriodicalIF":4.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415270/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ustilago maydis Nit2 Regulates Nitrate Utilisation During Biotrophy and Affects Amino Acid Metabolism of Galls Under Nitrogen Depletion. 氮耗竭条件下黑穗病菌Nit2调控瘤胃营养过程中硝酸盐利用及氨基酸代谢

IF 4.9 1区农林科学

Molecular plant pathology Pub Date : 2025-09-01 DOI: 10.1111/mpp.70148

Philipp L Lopinski, Christin Schulz, Alicia Fischer, Nadine Reichl, Timo Engelsdorf, Nadja Braun, Lars M Voll

{"title":"Ustilago maydis Nit2 Regulates Nitrate Utilisation During Biotrophy and Affects Amino Acid Metabolism of Galls Under Nitrogen Depletion.","authors":"Philipp L Lopinski, Christin Schulz, Alicia Fischer, Nadine Reichl, Timo Engelsdorf, Nadja Braun, Lars M Voll","doi":"10.1111/mpp.70148","DOIUrl":"10.1111/mpp.70148","url":null,"abstract":"In previous work, we have shown that the transcription factor Nit2 plays a major role in the utilisation of non-favoured nitrogen sources like nitrate, minor amino acids or nucleobases in saprotrophic sporidia of the basidiomycete corn smut fungus Ustilago maydis. Addressing the knowledge gap regarding how filamentous phytopathogens adapt to nitrogen limitation in the host plant, we employed Δnit2 mutants in the natural FB1 × FB2 background to identify Nit2-regulated genes during biotrophy. We further investigated the impact of Nit2 on the physiology of leaf galls in nitrogen-replete versus nitrogen-limited host plants by comparative RNA-Seq and metabolic steady state analysis. About one third of the fungal genes affected by Nit2 during biotrophy were involved in nitrogen metabolism and transport, only showing minor overlap to saprotrophic sporidia. Induction of the nitrate assimilation cluster was completely dependent on Nit2 during biotrophy. In nitrogen-limited host plants, Δnit2 leaf galls accumulated nitrate and showed reduced accumulation of the nitrogen-rich phloem transport amino acids asparagine and glutamine compared to wild-type galls. However, total protein content in galls and pathogenicity were comparable between fungal genotypes in both nitrogen regimes. The findings of our physiological and transcriptomic analysis demonstrate that nitrate utilisation is dispensable for U. maydis during biotrophy and can likely be actively compensated by increased utilisation of abundant organic nitrogen sources, like asparagine, GABA and glutamine in a partially Nit2-dependent fashion.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 9","pages":"e70148"},"PeriodicalIF":4.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12401940/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Accessory Chromosome Contributes to Virulence of Banana Infecting Fusarium oxysporum Tropical Race 4. 副染色体对香蕉感染尖孢镰刀菌热带小种4毒力的影响。

IF 4.9 1区农林科学

Molecular plant pathology Pub Date : 2025-09-01 DOI: 10.1111/mpp.70146

Jelmer Dijkstra, Anouk C van Westerhoven, Lucía Gómez-Gil, Carolina Aguilera-Galvez, Giuliana Nakasato-Tagami, Sebastien D Garnier, Masaya Yamazaki, Tsutomu Arie, Takashi Kamakura, Takayuki Arazoe, Antonio Di Pietro, Michael F Seidl, Gert H J Kema

{"title":"Accessory Chromosome Contributes to Virulence of Banana Infecting Fusarium oxysporum Tropical Race 4.","authors":"Jelmer Dijkstra, Anouk C van Westerhoven, Lucía Gómez-Gil, Carolina Aguilera-Galvez, Giuliana Nakasato-Tagami, Sebastien D Garnier, Masaya Yamazaki, Tsutomu Arie, Takashi Kamakura, Takayuki Arazoe, Antonio Di Pietro, Michael F Seidl, Gert H J Kema","doi":"10.1111/mpp.70146","DOIUrl":"10.1111/mpp.70146","url":null,"abstract":"Filamentous fungi have evolved compartmentalised genomes comprising conserved core regions and dynamic accessory regions, which are thought to drive adaptation to changing environments, including interactions with host organisms. Tropical Race 4 (TR4) is a lineage of banana-infecting Fusarium spp. and causes a devastating Fusarium wilt epidemic in the industrial banana cultivar Cavendish. A recent study showed that TR4 contains a single accessory chromosome (chromosome 12), which in some strains has undergone extensive internal duplications, tripling its size compared to other closely related strains. However, the contribution of this accessory chromosome to virulence is currently unknown. Here we show that the induced loss of accessory chromosome 12 in the TR4 reference strain II5 leads to reduced virulence on banana plants. Moreover, loss of chromosome 12 co-occurs with structural rearrangements of conserved core chromosomes. Together, our results provide new insights into the chromosome dynamics of the banana-infecting Fusarium TR4 lineage and highlight the importance of its unique accessory chromosome in virulence.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 9","pages":"e70146"},"PeriodicalIF":4.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12430104/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145054295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maize Diterpenoid Sensing via the Ste3 A-Pheromone Receptor Guide Oval Conidia of Colletotrichum graminicola to Host Roots. 通过Ste3 a -信息素受体引导玉米炭疽病卵圆形分生孢子到寄主根的二萜类传感。

IF 4.9 1区农林科学

Molecular plant pathology Pub Date : 2025-09-01 DOI: 10.1111/mpp.70155

Anina Y Rudolph, Carolin Schunke, Christoph Sasse, Luis Antelo, Jennifer Gerke, Gerhard H Braus, Stefanie Pöggeler, Daniela E Nordzieke

{"title":"Maize Diterpenoid Sensing via the Ste3 A-Pheromone Receptor Guide Oval Conidia of Colletotrichum graminicola to Host Roots.","authors":"Anina Y Rudolph, Carolin Schunke, Christoph Sasse, Luis Antelo, Jennifer Gerke, Gerhard H Braus, Stefanie Pöggeler, Daniela E Nordzieke","doi":"10.1111/mpp.70155","DOIUrl":"10.1111/mpp.70155","url":null,"abstract":"Colletotrichum graminicola, the maize anthracnose fungus, is known for its ability to invade above-ground tissues. This fungus forms two distinct asexual spore types in its life cycle, falcate conidia in acervuli on infected leaves and oval conidia in parenchyma cells in leaf and stem lesions. Our study reveals a previously unknown role for oval conidia in the infection of roots. We investigated whether root exudate from maize could redirect the growth of germlings generated by C. graminicola. Our results showed that only germlings derived from oval conidia were able to respond to root exudates, whereas those from falcate conidia did not. High-performance liquid chromatography/mass spectrometry (HPLC-MS) analyses combined with biological assays and genetic studies identified diterpenoids from maize as attractants perceived by the a-pheromone receptor CgSte3. We further explored the root-fungus interaction by analysing the germination of oval and falcate spores in soils of different composition. Oval conidia germinated rapidly under all conditions, whereas falcate conidia remained dormant and became highly vacuolated even in the presence of the host plant. Microscopic evaluation of root infection experiments showed that both conidia types attached to root material and formed penetration structures. However, only oval conidia enabled the pathogenic fungus to reach upper plant parts from infected roots. Our findings suggest a novel role for oval conidia in the infection of roots, highlighting the complexity of the anthracnose disease cycle.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 9","pages":"e70155"},"PeriodicalIF":4.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12445352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145086559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lettuce Big-Vein Associated Virus ORF3 Encodes a Functional 30K Movement Protein. 莴苣大静脉相关病毒ORF3编码一个功能性30K运动蛋白。

IF 4.9 1区农林科学

Molecular plant pathology Pub Date : 2025-09-01 DOI: 10.1111/mpp.70153

Willem E W Schravesande, Machiel V Cligge, Raoul Frijters, Adriaan Verhage, Harrold A van den Burg

{"title":"Lettuce Big-Vein Associated Virus ORF3 Encodes a Functional 30K Movement Protein.","authors":"Willem E W Schravesande, Machiel V Cligge, Raoul Frijters, Adriaan Verhage, Harrold A van den Burg","doi":"10.1111/mpp.70153","DOIUrl":"10.1111/mpp.70153","url":null,"abstract":"Movement proteins (MPs) modulate the size exclusion limit of plasmodesmata-membrane-lined channels connecting plant cells-thereby allowing cell-to-cell movement and systemic spread of plant viruses. The largest and arguably best-studied group of MPs is the 30K superfamily. Its family members share little sequence similarity, with only a handful of residues being well conserved. Yet, all family members appear to adopt the same jelly-roll protein fold structure. Lettuce big-vein associated virus (LBVaV), a member of the Rhabdoviridae family, is closely associated with lettuce big-vein disease (LBVD). It appears to facilitate the long-distance movement of Mirafiori lettuce big-vein virus (MiLBVV) in plants through an unknown mechanism. Notably, enhanced MiLBVV spread correlates with severe LBVD symptoms. Despite LBVaV having been known for decades, its proteins have not been studied in detail thus far. By using a combination of Alphafold2 structure modelling and FoldSeek structure-based homology searches, we managed to annotate all LBVaV open reading frames (ORFs), with ORF3 clustering with the 30K superfamily. While ORF3 is the most conserved protein sequence among the LBVaV-encoded ORFs, it shares only 5%-11% protein sequence identity with related MPs in the same genus. Microscopy studies confirmed that ORF3 locates at plasmodesmata, and in planta expression of ORF3 allowed cell-to-cell movement of two movement-impaired plant viruses. Thus, the Alphafold2-FoldSeek strategy allowed successful annotation of a plant viral genome even when viral proteins show little sequence similarity.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 9","pages":"e70153"},"PeriodicalIF":4.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12463573/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145150062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural Insights Into the Role of RxLR Effectors in the Arms Race Between Oomycetes and Plants. RxLR效应物在卵菌和植物军备竞赛中作用的结构见解。

IF 4.9 1区农林科学

Molecular plant pathology Pub Date : 2025-09-01 DOI: 10.1111/mpp.70138

Yang Liu, Yang Yang Zheng, Xin Ru Zhou, Hossein Ghanizadeh, Dong Li Wang, Pei Wen Wang, Mo Zhen Cheng, Jia Yin Liu, Xiu Ling Chen, Y Zhang, Jun Feng Liu, Ao Xue Wang

引用次数: 0

Mitochondrial Complex Member VdNuo1 Recruits Superoxide Dismutases VdSOD2/4 to Maintain Superoxide Anion Homeostasis During Pathogenesis in Verticillium dahliae. 在大丽花黄萎病发病过程中，线粒体复合体成员VdNuo1招募超氧化物歧化酶VdSOD2/4维持超氧化物阴离子稳态。

IF 4.9 1区农林科学

Molecular plant pathology Pub Date : 2025-09-01 DOI: 10.1111/mpp.70149

Huan Li, Ruo-Cheng Sheng, Ya-Hong Wang, Chen-Ning Zhang, Dan Wang, Steven J Klosterman, Dan-Dan Zhang, Krishna V Subbarao, Feng-Mao Chen, Jie-Yin Chen

{"title":"Mitochondrial Complex Member VdNuo1 Recruits Superoxide Dismutases VdSOD2/4 to Maintain Superoxide Anion Homeostasis During Pathogenesis in Verticillium dahliae.","authors":"Huan Li, Ruo-Cheng Sheng, Ya-Hong Wang, Chen-Ning Zhang, Dan Wang, Steven J Klosterman, Dan-Dan Zhang, Krishna V Subbarao, Feng-Mao Chen, Jie-Yin Chen","doi":"10.1111/mpp.70149","DOIUrl":"10.1111/mpp.70149","url":null,"abstract":"During oxidative phosphorylation, the leaked electrons generate superoxide anions to attack the mitochondrial inner membrane and impair mitochondrial activity. Three superoxide dismutases (SODs) are secreted to degrade host superoxide anions in Verticillium dahliae. However, the roles of mitochondrial SODs (mtSODs) in superoxide anion detoxification and in virulence are unknown in this fungus. We had previously shown that complex I VdNuo1 subunit mediates multiple biological functions and mitochondrial morphogenesis in V. dahliae. Here, we demonstrate that among the seven VdSODs of V. dahliae, only VdSOD2 and VdSOD4 were localised in the mitochondria and interact directly with VdNuo1. The VdNuo1 mutants, which exhibited mitochondrial inactivation in response to the superoxide anion inducer menadione, also displayed aberrant VdSODs transcription and SOD activity. VdSOD2 acted as a positive regulator of mitochondrial superoxide anion detoxification, and thus, its overexpression rescued the menadione-sensitive phenotypes of VdNuo1 mutants. In contrast, the increased tolerance of VdSOD2/VdSOD4 double mutants highlights that VdSOD4 negatively affects superoxide anion degradation. Thus, VdSOD2 and VdSOD4 cooperate with VdNuo1 to maintain SOD homeostasis for growth, mitochondrial superoxide anion detoxification, and virulence in V. dahliae. The two active superoxide anion scavengers CgSOD2 and CgSOD4 shared the same localisation and interaction model with CgNuo1 in Colletotrichum gloeosporioides. These results not only demonstrate the roles of mtSODs in V. dahliae and a novel conserved mechanism in which the respiratory chain couples with mtSODs to regulate superoxide anion metabolism in filamentous fungi, but also provide insights for the development of multisite fungicides to control phytopathogenic pathogens.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 9","pages":"e70149"},"PeriodicalIF":4.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12413312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145006345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Woody Host-Specific Type III Effector HopBL2 Is Essential for Pseudomonas savastanoi Virulence and Associates With Plasmodesmata. 木本寄主特异性III型效应物HopBL2对savastanoi假单胞菌毒力至关重要并与胞间连丝相关。

IF 4.9 1区农林科学

Molecular plant pathology Pub Date : 2025-09-01 DOI: 10.1111/mpp.70142

Alba Moreno-Pérez, Antonio Arroyo-Mateo, Luis Rodríguez-Moreno, Gitta Coaker, Cayo Ramos

{"title":"Woody Host-Specific Type III Effector HopBL2 Is Essential for Pseudomonas savastanoi Virulence and Associates With Plasmodesmata.","authors":"Alba Moreno-Pérez, Antonio Arroyo-Mateo, Luis Rodríguez-Moreno, Gitta Coaker, Cayo Ramos","doi":"10.1111/mpp.70142","DOIUrl":"10.1111/mpp.70142","url":null,"abstract":"The type III secretion system in Pseudomonas syringae complex pathogens delivers type III effectors (T3Es) into plant cells to manipulate host processes, enhance survival, and promote disease. While substantial research has focused on herbaceous pathogens, T3Es in strains infecting woody hosts are less understood. This study investigates the HopBL family of effectors in Pseudomonas savastanoi, a pathogen of woody plants. HopBL1 and HopBL2, core effectors in P. savastanoi, are restricted to phylogroup 3 strains of the P. syringae complex, all isolated from woody hosts. Phylogenetic analysis suggests recent horizontal acquisition of these effectors across multiple P. syringae pathovars, integrated into genomic islands flanked by mobile genetic elements. Structural analysis shows that both HopBL effectors contain SUMO protease and DNA-binding domains, with HopBL1 also possessing an ethylene-responsive motif, all characteristic of XopD from Xanthomonas spp. Despite low sequence identity, HopBL effectors exhibit structural similarity to XopD, with HopBL1 showing greater resemblance, particularly in the arrangement of these domains. Functional assays in olive and oleander revealed strain-specific contributions of HopBL1 and HopBL2 to virulence. In oleander, the natural host of P. savastanoi pv. nerii, mutation of either effector gene resulted in reduced symptom development. We show that HopBL2 localised predominantly to subnuclear foci and associated with plasmodesmata, with partial overlap observed along microtubules, suggesting a potential role in cytoskeleton manipulation. These findings underscore the importance of T3Es unique to P. syringae strains infecting woody hosts and their adaptation to modulate host cellular structures to promote disease.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 9","pages":"e70142"},"PeriodicalIF":4.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12413314/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145006363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RepA Protein of Citrus Chlorotic Dwarf-Associated Virus Impairs Perinuclear Chloroplast Clustering Induced by Lemon Chloroplast Malate Dehydrogenase. 柑桔绿矮秆相关病毒的RepA蛋白损害柠檬叶绿体苹果酸脱氢酶诱导的核周叶绿体聚集。

IF 4.9 1区农林科学

Molecular plant pathology Pub Date : 2025-08-01 DOI: 10.1111/mpp.70133

Yuan Chen, Jinfa Zhao, Jiajun Wang, Qi Zhang, Mengji Cao, Yan Zhou

引用次数: 0