Molecular plant pathology最新文献

{"title":"Importin α2 participates in RNA interference against bamboo mosaic virus accumulation in Nicotiana benthamiana via NbAGO10a-mediated small RNA clearance","authors":"Jiun-Da Wang, Yau-Heiu Hsu, Yun-Shien Lee, Na-Sheng Lin","doi":"10.1111/mpp.13422","DOIUrl":"https://doi.org/10.1111/mpp.13422","url":null,"abstract":"Karyopherins, the nucleocytoplasmic transporters, participate in multiple RNA silencing stages by transporting associated proteins into the nucleus. Importin α is a member of karyopherins and has been reported to facilitate virus infection via nuclear import of viral proteins. Unlike other RNA viruses, silencing of importin α2 (α2<i>i</i>) by virus-induced gene silencing (VIGS) boosted the titre of bamboo mosaic virus (BaMV) in protoplasts, and inoculated and systemic leaves of <i>Nicotiana benthamiana</i>. The enhanced BaMV accumulation in importin α2<i>i</i> plants was linked to reduced levels of RDR6-dependent secondary virus-derived small-interfering RNAs (vsiRNAs). Small RNA-seq revealed importin α2 silencing did not affect the abundance of siRNAs derived from host mRNAs but significantly reduced the 21 and 22 nucleotide vsiRNAs in BaMV-infected plants. Deletion of BaMV TGBp1, an RNA silencing suppressor, compromised importin α2<i>i</i>-mediated BaMV enhancement. Moreover, silencing of importin α2 upregulated NbAGO10a, a proviral protein recruited by TGBp1 for BaMV vsiRNAs clearance, but hindered the nuclear import of NbAGO10a. Taken together, these results indicate that importin α2 acts as a negative regulator of BaMV invasion by controlling the expression and nucleocytoplasmic shuttling of NbAGO10a, which removes vsiRNAs via the TGBp1-NbAGO10a-SDN1 pathway. Our findings reveal the hidden antiviral mechanism of importin α2 in countering BaMV infection in <i>N</i>. <i>benthamiana</i>.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"116 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139515527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to ‘Different epitopes of Ralstonia solanacearum effector RipAW are recognized by two Nicotiana species and trigger immune responses’","authors":"","doi":"10.1111/mpp.13420","DOIUrl":"https://doi.org/10.1111/mpp.13420","url":null,"abstract":"<p>Niu Y, Fu S, Chen G, Wang H, Wang Y, Hu J, Jin X, Zhang M, Lu M, He Y, Wang D, Chen Y, Zhang Y, Coll N, Valls M, Zhao C, Chen Q and Lu H. Different epitopes of <i>Ralstonia solanacearum</i> effector RipAW are recognized by two <i>Nicotiana</i> species and trigger immune responses. <i>Molecular Plant Pathology</i> 2022;23:188–203</p>\u0000<p>In the authorship section, the affiliations ‘Dongdong Wang², Yong Zhang^3,4, Núria S. Coll⁵, Marc Valls^5,6, Qin Chen² and Haibin Lu^1,7’ (Author's affiliations) were incorrect. They should have read ‘Dongdong Wang³, Yong Zhang^4,5, Núria S. Coll⁶, Marc Valls^6,7, Qin Chen³ and Haibin Lu^1,2’</p>\u0000<p>We apologize for these errors.</p>","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139515656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Nicotiana benthamiana phosphatidylinositol 4-kinase type II regulates chilli leaf curl virus pathogenesis”","authors":"","doi":"10.1111/mpp.13421","DOIUrl":"https://doi.org/10.1111/mpp.13421","url":null,"abstract":"<p>Mansi, Kushwaha, N.K., Singh, A.K., Karim, M.J. and Chakraborty, S. (2019) <i>Nicotiana benthamiana</i> phosphatidylinositol 4-kinase type II regulates chilli leaf curl virus pathogenesis. <i>Molecular Plant Pathology</i>, 20(10), 1408–1424. https://doi.org/10.1111/mpp.12846.</p>\u0000<p>In the published version of this article in Figure 6, panels (d) and (e) were mistakenly used for both the NbPI4KII-mGFP + Rep1-180-DsRed and NbPI4KII-mGFP + Rep181-361-DsRed images. The two panels showing individual microscopy images with the NbPI4KII-mGFP + Rep1-180-DsRed and NbPI4KII-mGFP + Rep181-361-DsRed are highly identical; hence this mistake happened during figure assembly. This inadvertent figure error does not in any way alter the interpretations or the conclusions drawn in the manuscript. The corrected Figure 6 is shown below.</p>\u0000<figure><picture>\u0000<source media=\"(min-width: 1650px)\" srcset=\"/cms/asset/dabc6575-0d18-42a2-85e5-c4cf0541aa42/mpp13421-fig-0001-m.jpg\"/><img alt=\"Details are in the caption following the image\" data-lg-src=\"/cms/asset/dabc6575-0d18-42a2-85e5-c4cf0541aa42/mpp13421-fig-0001-m.jpg\" loading=\"lazy\" src=\"/cms/asset/2814bb6c-0e51-4747-9329-cfc3c261cd9a/mpp13421-fig-0001-m.png\" title=\"Details are in the caption following the image\"/></picture><figcaption>\u0000<div><strong>FIGURE 6</strong><div>Open in figure viewer<i aria-hidden=\"true\"></i><span>PowerPoint</span></div>\u0000</div>\u0000<div>Colocalization study of NbPI4K-mGFP with Rep-DsRed and its mutant. Localization of NbPI4KII:mGFP with (a, b) Rep-DsRed, (c) Rep_1-120-DsRed, (d) Rep_1-180-DsRed and (e) Rep_181-361-DsRed. Scale bar = 20 μm.</div>\u0000</figcaption>\u0000</figure>\u0000<p>We apologize for this error.</p>","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"8 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139515837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"WRR4B contributes to a broad-spectrum disease resistance against powdery mildew in Arabidopsis","authors":"Shuangshuang Mei, Yuxin Song, Zuer Zhang, Haitao Cui, Shuguo Hou, Weiguo Miao, Wei Rong","doi":"10.1111/mpp.13415","DOIUrl":"https://doi.org/10.1111/mpp.13415","url":null,"abstract":"<i>Oidium heveae</i> HN1106, a powdery mildew (PM) that infects rubber trees, has been found to trigger disease resistance in <i>Arabidopsis thaliana</i> through ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1)-, PHYTOALEXIN DEFICIENT 4 (PAD4)- and salicylic acid (SA)-mediated signalling pathways. In this study, a typical <i>TOLL-INTERLEUKIN 1 RECEPTOR, NUCLEOTIDE-BINDING, LEUCINE-RICH REPEAT</i> (<i>TIR-NB-LRR</i>)-encoding gene, <i>WHITE RUST RESISTANCE 4</i> (<i>WRR4B</i>), was identified to be required for the resistance against <i>O. heveae</i> in <i>Arabidopsis</i>. The expression of <i>WRR4B</i> was upregulated by <i>O. heveae</i> inoculation, and <i>WRR4B</i> positively regulated the expression of genes involved in SA biosynthesis, such as <i>EDS1</i>, <i>PAD4</i>, <i>ICS1</i> (<i>ISOCHORISMATE SYNTHASE 1</i>), <i>SARD1</i> (<i>SYSTEMIC-ACQUIRED RESISTANCE DEFICIENT 1</i>) and <i>CBP60g</i> (<i>CALMODULIN-BINDING PROTEIN 60 G</i>). Furthermore, WRR4B triggered self-amplification, suggesting that WRR4B mediated plant resistance through taking part in the SA-based positive feedback loop. In addition, WRR4B induced an <i>EDS1</i>-dependent hypersensitive response in <i>Nicotiana benthamiana</i> and contributed to disease resistance against three other PM species: <i>Podosphaera xanthii</i>, <i>Erysiphe quercicola</i> and <i>Erysiphe neolycopersici</i>, indicating that <i>WRR4B</i> is a broad-spectrum disease resistance gene against PMs.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"86 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139410955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infection-specific transcriptional patterns of the maize pathogen Cochliobolus heterostrophus unravel genes involved in asexual development and virulence","authors":"Huilin Yu, Jiyue Zhang, Jinyu Fan, Wantong Jia, Yanan Lv, Hongyu Pan, Xianghui Zhang","doi":"10.1111/mpp.13413","DOIUrl":"https://doi.org/10.1111/mpp.13413","url":null,"abstract":"Southern corn leaf blight (SCLB) caused by <i>Cochliobolus heterostrophus</i> is a destructive disease that threatens global maize (<i>Zea mays</i>) production. Despite many studies being conducted, very little is known about molecular processes employed by the pathogen during infection. There is a need to understand the fungal arms strategy and identify novel functional genes as targets for fungicide development. Transcriptome analysis based on RNA sequencing was carried out across conidia germination and host infection by <i>C. heterostrophus</i>. The present study revealed major changes in <i>C. heterostrophus</i> gene expression during host infection. Several differentially expressed genes (DEGs) induced during <i>C. heterostrophus</i> infection could be involved in the biosynthesis of secondary metabolites, peroxisome, energy metabolism, amino acid degradation and oxidative phosphorylation. In addition, histone acetyltransferase, secreted proteins, peroxisomal proteins, NADPH oxidase and transcription factors were selected for further functional validation. Here, we demonstrated that histone acetyltransferases (Hat2 and Rtt109), secreted proteins (Cel61A and Mep1), peroxisomal proteins (Pex11A and Pex14), NADPH oxidases (NoxA, NoxD and NoxR) and transcription factors (Crz1 and MtfA) play essential roles in <i>C. heterostrophus</i> conidiation, stress adaption and virulence. Taken together, our study revealed major changes in gene expression associated with <i>C. heterostrophus</i> infection and identified a diverse repertoire of genes critical for successful infection.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"1 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139411022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytophthora infestans RXLR effector Pi23014 targets host RNA-binding protein NbRBP3a to suppress plant immunity","authors":"Wanyue Li, Zeming Liu, Yuli Huang, Jie Zheng, Yang Yang, Yimeng Cao, Liwen Ding, Yuling Meng, Weixing Shan","doi":"10.1111/mpp.13416","DOIUrl":"https://doi.org/10.1111/mpp.13416","url":null,"abstract":"<i>Phytophthora infestans</i> is a destructive oomycete that causes the late blight of potato and tomato worldwide. It secretes numerous small proteins called effectors in order to manipulate host cell components and suppress plant immunity. Identifying the targets of these effectors is crucial for understanding <i>P. infestans</i> pathogenesis and host plant immunity. In this study, we show that the virulence RXLR effector Pi23014 of <i>P. infestans</i> targets the host nucleus and chloroplasts. By using a liquid chromatogrpahy-tandem mass spectrometry assay and co-immunoprecipitation assasys, we show that it interacts with NbRBP3a, a putative glycine-rich RNA-binding protein. We confirmed the co-localization of Pi23014 and NbRBP3a within the nucleus, by using bimolecular fluorescence complementation. Reverse transcription-quantitative PCR assays showed that the expression of <i>NbRBP3a</i> was induced in <i>Nicotiana benthamiana</i> during <i>P. infestans</i> infection and the expression of marker genes for multiple defence pathways were significantly down-regulated in <i>NbRBP3</i>-silenced plants compared with <i>GFP</i>-silenced plants. <i>Agrobacterium tumefaciens</i>-mediated transient overexpression of <i>NbRBP3a</i> significantly enhanced plant resistance to <i>P. infestans</i>. Mutations in the N-terminus RNA recognition motif (RRM) of NbRBP3a abolished its interaction with Pi23014 and eliminated its capability to enhance plant resistance to leaf colonization by <i>P. infestans</i>. We further showed that silencing <i>NbRBP3</i> reduced photosystem II activity, reduced host photosynthetic efficiency, attenuated Pi23014-mediated suppression of cell death triggered by <i>P. infestans</i> pathogen-associated molecular pattern elicitor <i>INF1</i>, and suppressed plant immunity.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"4 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139411060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic variations in root tissues and rhizosphere soils of weak host plants potently lead to distinct host status and chemotaxis regulation of Meloidogyne incognita in intercropping.","authors":"Xu Zhang, Mengyuan Song, Lihong Gao, Yongqiang Tian","doi":"10.1111/mpp.13396","DOIUrl":"10.1111/mpp.13396","url":null,"abstract":"<p><p>Root-knot nematodes (RKNs) inflict extensive damage to global agricultural production. Intercropping has been identified as a viable agricultural tool for combating RKNs, but the mechanisms by which intercropped plants modulate RKN parasitism are still not well understood. Here, we focus on the cucumber-amaranth intercropping system. We used a range of approaches, including the attraction assay, in vitro RNA interference (RNAi), untargeted metabolomics, and hairy root transformation, to unveil the mechanisms by which weak host plants regulate Meloidogyne incognita chemotaxis towards host plants and control infection. Amaranth roots showed a direct repellence to M. incognita through disrupting its chemotaxis. The in vitro RNAi assay demonstrated that the Mi-flp-1 and Mi-flp-18 genes (encoding FMRFamide-like peptides) regulated M. incognita chemotaxis towards cucumber and controlled infection. Moreover, M. incognita infection stimulated cucumber and amaranth to accumulate distinct metabolites in both root tissues and rhizosphere soils. In particular, naringenin and salicin, enriched specifically in amaranth rhizosphere soils, inhibited the expression of Mi-flp-1 and Mi-flp-18. In addition, overexpression of genes involved in the biosynthesis of pantothenic acid and phloretin, both of which were enriched specifically in amaranth root tissues, delayed M. incognita development in cucumber hairy roots. Together, our results reveal that both the distinct host status and disruption of chemotaxis contribute to M. incognita inhibition in intercropping.</p>","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":" ","pages":"e13396"},"PeriodicalIF":4.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10782644/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41205379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The nanovirus U2 protein suppresses RNA silencing via three conserved cysteine residues.","authors":"Dankan Yan, Kelei Han, Yuwen Lu, Jiejun Peng, Shaofei Rao, Guanwei Wu, Yong Liu, Jianping Chen, Hongying Zheng, Fei Yan","doi":"10.1111/mpp.13394","DOIUrl":"10.1111/mpp.13394","url":null,"abstract":"<p><p>Nanoviruses have multipartite, circular, single-stranded DNA genomes and cause huge production losses in legumes and other crops. No viral suppressor of RNA silencing (VSR) has yet been reported from a member of the genus Nanovirus. Here, we demonstrate that the nanovirus U2 protein is a VSR. The U2 protein of milk vetch dwarf virus (MDV) suppressed the silencing of the green fluorescent protein (GFP) gene induced by single-stranded and double-stranded RNA, and the systemic spread of the GFP silencing signal. An electrophoretic mobility shift assay showed that the U2 protein was able to bind double-stranded 21-nucleotide small interfering RNA (siRNA). The cysteine residues at positions 43, 79 and 82 in the MDV U2 protein are critical to its nuclear localization, self-interaction and siRNA-binding ability, and were essential for its VSR activity. In addition, expression of the U2 protein via a potato virus X vector induced more severe necrosis symptoms in Nicotiana benthamiana leaves. The U2 proteins of other nanoviruses also acted as VSRs, and the three conserved cysteine residues were indispensable for their VSR activity.</p>","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":" ","pages":"e13394"},"PeriodicalIF":4.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10782648/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41205381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Pseudomonas syringae pv. tomato biofilm-like aggregate formation in susceptible and PTI-responding Arabidopsis thaliana.","authors":"Wantao N Xiao, Garrett M Nunn, Angela B Fufeng, Natalie Belu, Rowan K Brookman, Abdul Halim, Evan C Krysmanski, Robin K Cameron","doi":"10.1111/mpp.13403","DOIUrl":"10.1111/mpp.13403","url":null,"abstract":"<p><p>Bacterial biofilm-like aggregates have been observed in plants, but their role in pathogenicity is underinvestigated. In the present study, we observed that extracellular DNA and polysaccharides colocalized with green fluorescent protein (GFP)-expressing Pseudomonas syringae pv. tomato (Pst) aggregates in Arabidopsis leaves, suggesting that Pst aggregates are biofilms. GFP-expressing Pst, Pst ΔalgU ΔmucAB (Pst algU mutant), and Pst ΔalgD ΔalgU ΔmucAB (Pst algU algD mutant) were examined to explore the roles of (1) alginate, a potential biofilm component; (2) Pst AlgU, thought to regulate alginate biosynthesis and some type III secretion system effector genes; and (3) intercellular salicylic acid (SA) accumulation during pathogen-associated molecular pattern-triggered immunity (PTI). Pst formed extensive aggregates in susceptible plants, whereas aggregate numbers and size were reduced in Pst algU and Pst algD algU mutants, and both multiplied poorly in planta, suggesting that aggregate formation contributes to Pst success in planta. However, in SA-deficient sid2-2 plants, Pst algD algU mutant multiplication and aggregate formation were partially restored, suggesting plant-produced SA contributes to suppression of Pst aggregate formation. Pst algD algU mutants formed fewer and smaller aggregates than Pst algU mutants, suggesting both AlgU and AlgD contribute to Pst aggregate formation. Col-0 plants accumulated low levels of SA in response to Pst and both mutants (Pst algU and Pst algD algU), suggesting the regulatory functions of AlgU are not involved in suppressing SA-mediated plant defence. Plant PTI was associated with highly reduced Pst aggregate formation and accumulation of intercellular SA in flg22-induced PTI-responding wild-type Col-0, but not in PTI-incompetent fls2, suggesting intercellular SA accumulation by Arabidopsis contributes to suppression of Pst biofilm-like aggregate formation during PTI.</p>","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":" ","pages":"e13403"},"PeriodicalIF":4.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10799205/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138291375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cassava molecular genetics and genomics for enhanced resistance to diseases and pests.","authors":"Valentine Otang Ntui, Jaindra Nath Tripathi, Samwel Muiruri Kariuki, Leena Tripathi","doi":"10.1111/mpp.13402","DOIUrl":"10.1111/mpp.13402","url":null,"abstract":"<p><p>Cassava (Manihot esculenta) is one of the most important sources of dietary calories in the tropics, playing a central role in food and economic security for smallholder farmers. Cassava production is highly constrained by several pests and diseases, mostly cassava mosaic disease (CMD) and cassava brown streak disease (CBSD). These diseases cause significant yield losses, affecting food security and the livelihoods of smallholder farmers. Developing resistant varieties is a good way of increasing cassava productivity. Although some levels of resistance have been developed for some of these diseases, there is observed breakdown in resistance for some diseases, such as CMD. A frequent re-evaluation of existing disease resistance traits is required to make sure they are still able to withstand the pressure associated with pest and pathogen evolution. Modern breeding approaches such as genomic-assisted selection in addition to biotechnology techniques like classical genetic engineering or genome editing can accelerate the development of pest- and disease-resistant cassava varieties. This article summarizes current developments and discusses the potential of using molecular genetics and genomics to produce cassava varieties resistant to diseases and pests.</p>","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":" ","pages":"e13402"},"PeriodicalIF":4.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10788594/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71483722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}