Molecular plant pathology最新文献

{"title":"Mutation of OsCDS5 confers broad-spectrum disease resistance in rice","authors":"Qiping Sun, Yongxin Xiao, Le Song, Lei Yang, Yin Wang, Wei Yang, Qun Yang, Kabin Xie, Meng Yuan, Guotian Li","doi":"10.1111/mpp.13430","DOIUrl":"https://doi.org/10.1111/mpp.13430","url":null,"abstract":"Phospholipids are important components of biological membranes, participating in various biological processes, including plant development and responses to biotic and abiotic stresses. A previous study showed that mutation of the rice <i>OsCDS5</i> (<span style=\"text-decoration:underline\">C</span>DP-<span style=\"text-decoration:underline\">D</span>AG <span style=\"text-decoration:underline\">S</span>ynthase) gene alters lipid metabolism, causing enhanced abiotic stress responses, yellowing of leaves at the seedling stage and delayed plant development. Here, we observed that the <i>Oscds5</i> mutant shows enhanced resistance to rice blast, bacterial blight and bacterial leaf streak. Mutation of <i>OsCDS5</i> promotes production of reactive oxygen species and increases the expression level of multiple defence-related genes. Transcriptomic analyses indicate that genes involved in responses to stress, biotic/abiotic stimuli and metabolic processes are highly upregulated and enriched in mutant <i>Oscds5</i>. Metabolomic analyses showed that differential metabolites were enriched in the lipid metabolic and tryptophan metabolic pathways. The decreased level of phosphatidylinositol and increased level of serotonin probably contribute to enhanced disease resistance of the <i>Oscds5</i> mutant. Taken together, mutation of <i>OsCDS5</i> enhances abiotic and biotic stress responses, and <i>OsCDS5</i> may be a promising target for genetic engineering to enhance the resilience of rice to abiotic and biotic stresses simultaneously.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139753023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant–pathogen interaction with root rot of Panax notoginseng as a model: Insight into pathogen pathogenesis, plant defence response and biological control","authors":"Jianbin Li, Mingtao Ai, Jiae Hou, Peiqi Zhu, Xiuming Cui, Qian Yang","doi":"10.1111/mpp.13427","DOIUrl":"https://doi.org/10.1111/mpp.13427","url":null,"abstract":"Plant diseases are a major threat affecting the sustainability of global agriculture. Although the breeding of new resistant cultivars is considered to be the primary approach to prevent and control plant diseases, it is dependent on an in-depth understanding of plant–pathogen interactions. At present, we have an in-depth understanding of the interactions between model plants and pathogens, such as <i>Arabidopsis thaliana</i> and rice, but we are still in the beginning stage for more non-model plants (e.g., medicinal plants). <i>Panax notoginseng</i> is the primary source of the high-value active ingredient triterpenoid saponins. Root rot disease in <i>P. notoginseng</i> has attracted research attention because of its high destructiveness. Understanding the infection stages and strategies of pathogens, plant resistance mechanisms and induced plant defence against pathogens is essential to support agricultural sustainable development of <i>P. notoginseng</i>. Here, we review and summarize, with root rot of <i>P. notoginseng</i> as a model, the current knowledge of plant–pathogen interaction, and feasability of use of microorganisms and secondary metabolites as sources of biological control agents at a low cost. Finally, we also discuss the importance of plant–pathogen interactions in resistance breeding, thereby providing a new strategy to develop green agriculture for non-model plants.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"98 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139773238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A natural single-nucleotide polymorphism in the CAMTA3 transcription factor regulates its function and transcription of its target genes","authors":"Kasavajhala V. S. K. Prasad, Amira A. E. Abdel-Hameed, Anireddy S. N. Reddy","doi":"10.1111/mpp.13428","DOIUrl":"https://doi.org/10.1111/mpp.13428","url":null,"abstract":"CAMTA3, a Ca²⁺/calmodulin-binding transcription factor, is a key regulator of plant immunity in <i>Arabidopsis</i>. Here, we identified a novel naturally occurring single-nucleotide polymorphism that results in a missense nonconservative mutation (<i>CAMTA3</i>_<i>H386D</i>) in many <i>Arabidopsis</i> ecotypes. This region of CAMTA3 is not part of any previously characterized regulatory domains. To study the consequence of this change on the function of CAMTA3, we introduced the <i>CAMTA3</i>_<i>H386D</i> into <i>camta3</i>, a loss-of-function mutant that exhibits a constitutive cell death phenotype, chlorotic lesions on leaves, and reduced plant size. Phenotypic and molecular analysis of these lines indicated that the expression of <i>CAMTA3</i>_<i>H386D</i> in the <i>camta3</i> mutant did not complement the mutant phenotypes. Also, the ecotypes containing the <i>CAMTA3</i>_<i>H386D</i> exhibited <i>camta3</i> phenotypes. Marker genes associated with salicylic acid biosynthesis and pathogen response were upregulated in the <i>CAMTA3</i>_<i>H386D</i> lines and the <i>Arabidopsis</i> accessions 7127 (Est-1) and 9941 (Fei-0), as in <i>camta3</i>, indicating that H386D mutation alters CAMTA3 activity in regulating the expression of known target genes. In <i>Nicotiana benthamiana</i> transient expression assays, <i>CAMTA3</i>_<i>H386D</i> failed to induce the expression of a luciferase reporter gene driven by the rapid stress-responsive elements (RSRE) that contain the known binding sites of CAMTA3, suggesting that CAMTA3_H386D mutation impairs its ability to activate its target genes. Transgenic lines and tested natural accessions expressing CAMTA3_H386D showed enhanced levels of H₂O₂ and increased resistance to the bacterial pathogen <i>Pseudomonas syringae</i> pv. <i>tomato</i> DC3000. Collectively, our results indicate that the H386D mutation in a previously unknown regulatory region of CAMTA3 is essential for its function.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"14 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139773281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"UvHOS3-mediated histone deacetylation is essential for virulence and negatively regulates ustilaginoidin biosynthesis in Ustilaginoidea virens.","authors":"Bo Wang, Guohua Duan, Ling Liu, Zhaoyi Long, Xiaolong Bai, Mingming Ou, Peiying Wang, Du Jiang, Dayong Li, Wenxian Sun","doi":"10.1111/mpp.13429","DOIUrl":"10.1111/mpp.13429","url":null,"abstract":"<p><p>Ustilaginoidea virens is the causal agent of rice false smut, which has recently become one of the most important rice diseases worldwide. Ustilaginoidins, a major type of mycotoxins produced in false smut balls, greatly deteriorates grain quality. Histone acetylation and deacetylation are involved in regulating secondary metabolism in fungi. However, little is yet known on the functions of histone deacetylases (HDACs) in virulence and mycotoxin biosynthesis in U. virens. Here, we characterized the functions of the HDAC UvHOS3 in U. virens. The ΔUvhos3 deletion mutant exhibited the phenotypes of retarded growth, increased mycelial branches and reduced conidiation and virulence. The ΔUvhos3 mutants were more sensitive to sorbitol, sodium dodecyl sulphate and oxidative stress/H₂ O₂ . ΔUvhos3 generated significantly more ustilaginoidins. RNA-Seq and metabolomics analyses also revealed that UvHOS3 is a key negative player in regulating secondary metabolism, especially mycotoxin biosynthesis. Notably, UvHOS3 mediates deacetylation of H3 and H4 at H3K9, H3K18, H3K27 and H4K8 residues. Chromatin immunoprecipitation assays indicated that UvHOS3 regulates mycotoxin biosynthesis, particularly for ustilaginoidin and sorbicillinoid production, by modulating the acetylation level of H3K18. Collectively, this study deepens the understanding of molecular mechanisms of the HDAC UvHOS3 in regulating virulence and mycotoxin biosynthesis in phytopathogenic fungi.</p>","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 2","pages":"e13429"},"PeriodicalIF":4.9,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10866089/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139730031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The schizotrophic lifestyle of Sclerotinia sclerotiorum.","authors":"Qingna Shang, Daohong Jiang, Jiatao Xie, Jiasen Cheng, Xueqiong Xiao","doi":"10.1111/mpp.13423","DOIUrl":"10.1111/mpp.13423","url":null,"abstract":"<p><p>Sclerotinia sclerotiorum is a cosmopolitan and typical necrotrophic phytopathogenic fungus that infects hundreds of plant species. Because no cultivars highly resistant to S. sclerotiorum are available, managing Sclerotinia disease caused by S. sclerotiorum is still challenging. However, recent studies have demonstrated that S. sclerotiorum has a beneficial effect and can live mutualistically as an endophyte in graminaceous plants, protecting the plants against major fungal diseases. An in-depth understanding of the schizotrophic lifestyle of S. sclerotiorum during interactions with plants under different environmental conditions will provide new strategies for controlling fungal disease. In this review, we summarize the pathogenesis mechanisms of S. sclerotiorum during its attack of host plants as a destructive pathogen and discuss its lifestyle as a beneficial endophytic fungus.</p>","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 2","pages":"e13423"},"PeriodicalIF":4.8,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10895550/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139972709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The secreted feruloyl esterase of Verticillium dahliae modulates host immunity via degradation of GhDFR.","authors":"Yajuan Wang, Xiwen Liao, Wenjing Shang, Jun Qin, Xiangming Xu, Xiaoping Hu","doi":"10.1111/mpp.13431","DOIUrl":"10.1111/mpp.13431","url":null,"abstract":"<p><p>Feruloyl esterase (ferulic acid esterase, FAE) is an essential component of many biological processes in both eukaryotes and prokaryotes. This research aimed to investigate the role of FAE and its regulation mechanism in plant immunity. We identified a secreted feruloyl esterase VdFAE from the hemibiotrophic plant pathogen Verticillium dahliae. VdFAE acted as an important virulence factor during V. dahliae infection, and triggered plant defence responses, including cell death in Nicotiana benthamiana. Deletion of VdFAE led to a decrease in the degradation of ethyl ferulate. VdFAE interacted with Gossypium hirsutum protein dihydroflavanol 4-reductase (GhDFR), a positive regulator in plant innate immunity, and promoted the degradation of GhDFR. Furthermore, silencing of GhDFR led to reduced resistance of cotton plants against V. dahliae. The results suggested a fungal virulence strategy in which a fungal pathogen secretes FAE to interact with host DFR and interfere with plant immunity, thereby promoting infection.</p>","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 2","pages":"e13431"},"PeriodicalIF":4.9,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10866084/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139730069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous application of pectin triggers stomatal closure and immunity in Arabidopsis.","authors":"Cheng Zhang, Charles Tetteh, Sheng Luo, Pinyuan Jin, Xingqian Hao, Min Sun, Nan Fang, Yingjun Liu, Huajian Zhang","doi":"10.1111/mpp.13438","DOIUrl":"10.1111/mpp.13438","url":null,"abstract":"<p><p>Pectin has been extensively studied in animal immunity, and exogenous pectin as a food additive can provide protection against inflammatory bowel disease. However, the utility of pectin to improve immunity in plants is still unstudied. Here, we found exogenous application of pectin triggered stomatal closure in Arabidopsis in a dose- and time-dependent manner. Additionally, pectin activated peroxidase and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase to produce reactive oxygen species (ROS), which subsequently increased cytoplasmic Ca²⁺ concentration ([Ca²⁺ ]_cyt ) and was followed by nitric oxide (NO) production, leading to stomatal closure in an abscisic acid (ABA) and salicylic acid (SA) signalling-dependent mechanism. Furthermore, pectin enhanced the disease resistance to Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) with mitogen-activated protein kinases (MPKs) MPK3/6 activated and upregulated expression of defence-responsive genes in Arabidopsis. These results suggested that exogenous pectin-induced stomatal closure was associated with ROS and NO production regulated by ABA and SA signalling, contributing to defence against Pst DC3000 in Arabidopsis.</p>","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 2","pages":"e13438"},"PeriodicalIF":4.9,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10887356/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139932020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytoplasma: A plant pathogen that cannot be ignored in agricultural production-Research progress and outlook.","authors":"Ruotong Wang, Bixin Bai, Danyang Li, Jingke Wang, Weijie Huang, Yunfeng Wu, Lei Zhao","doi":"10.1111/mpp.13437","DOIUrl":"10.1111/mpp.13437","url":null,"abstract":"<p><p>Phytoplasmas are phloem-restricted plant-pathogenic bacteria transmitted by insects. They cause diseases in a wide range of host plants, resulting in significant economic and ecological losses worldwide. Research on phytoplasmas has a long history, with significant progress being made in the past 30 years. Notably, with the rapid development of phytoplasma research, scientists have identified the primary agents involved in phytoplasma transmission, established classification and detection systems for phytoplasmas, and 243 genomes have been sequenced and assembled completely or to draft quality. Multiple possible phytoplasma effectors have been investigated, elucidating the molecular mechanisms by which phytoplasmas manipulate their hosts. This review summarizes recent advances in phytoplasma research, including identification techniques, host range studies, whole- or draft-genome sequencing, effector pathogenesis and disease control methods. Additionally, future research directions in the field of phytoplasma research are discussed.</p>","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 2","pages":"e13437"},"PeriodicalIF":4.9,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10887288/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139932021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to Cassava molecular genetics and genomics for enhanced resistance to diseases and pests.","authors":"","doi":"10.1111/mpp.13432","DOIUrl":"10.1111/mpp.13432","url":null,"abstract":"","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 2","pages":"e13432"},"PeriodicalIF":4.9,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10853577/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139707259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Turnip mosaic virus NIb weakens the function of eukaryotic translation initiation factor 6 facilitating viral infection in Nicotiana benthamiana.","authors":"Ziqiang Chen, Feng Wang, Binghua Chen, Guanwei Wu, Dagang Tian, Quan Yuan, Shiyou Qiu, Yushan Zhai, Jianping Chen, Hongying Zheng, Fei Yan","doi":"10.1111/mpp.13434","DOIUrl":"10.1111/mpp.13434","url":null,"abstract":"<p><p>Viruses rely completely on host translational machinery to produce the proteins encoded by their genes. Controlling translation initiation is important for gaining translational advantage in conflicts between the host and virus. The eukaryotic translation initiation factor 4E (eIF4E) has been reported to be hijacked by potyviruses for virus multiplication. The role of translation regulation in defence and anti-defence between plants and viruses is not well understood. We report that the transcript level of eIF6 was markedly increased in turnip mosaic virus (TuMV)-infected Nicotiana benthamiana. TuMV infection was impaired by overexpression of N. benthamiana eIF6 (NbeIF6) either transiently expressed in leaves or stably expressed in transgenic plants. Polysome profile assays showed that overexpression of NbeIF6 caused the accumulation of 40S and 60S ribosomal subunits, the reduction of polysomes, and also compromised TuMV UTR-mediated translation, indicating a defence role for upregulated NbeIF6 during TuMV infection. However, the polysome profile in TuMV-infected leaves was not identical to that in leaves overexpressing NbeIF6. Further analysis showed that TuMV NIb protein, the RNA-dependent RNA polymerase, interacted with NbeIF6 and interfered with its effect on the ribosomal subunits, suggesting that NIb might have a counterdefence role. The results propose a possible regulatory mechanism at the translation level during plant-virus interaction.</p>","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 2","pages":"e13434"},"PeriodicalIF":4.8,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10883789/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139932022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}