Molecular plant pathology最新文献_第3页

XopM, An FFAT Motif-Containing Type III Effector Protein From Xanthomonas, Suppresses MTI Responses at the Plant Plasma Membrane. 含FFAT基序的黄单胞菌III型效应蛋白XopM抑制植物质膜上的MTI反应。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-12-01 DOI: 10.1111/mpp.70038

Charlotte Brinkmann, Jennifer Bortlik, Margot Raffeiner, Manuel González-Fuente, Linus F Börnke, Suayib Üstün, Frederik Börnke

{"title":"XopM, An FFAT Motif-Containing Type III Effector Protein From Xanthomonas, Suppresses MTI Responses at the Plant Plasma Membrane.","authors":"Charlotte Brinkmann, Jennifer Bortlik, Margot Raffeiner, Manuel González-Fuente, Linus F Börnke, Suayib Üstün, Frederik Börnke","doi":"10.1111/mpp.70038","DOIUrl":"10.1111/mpp.70038","url":null,"abstract":"Many gram-negative pathogenic bacteria use type III effector proteins (T3Es) as essential virulence factors to suppress host immunity and to cause disease. However, in many cases the molecular function of T3Es remains unknown. The plant pathogen Xanthomonas campestris pv. vesicatoria (Xcv) is the causal agent of bacterial spot disease on tomato and pepper plants and is known to translocate around 36 T3Es into its host cell, which collectively suppress plant defence and promote infection. XopM is an Xcv core T3E with unknown function that has no similarity to any other known protein. We found that XopM interacts with vesicle-associated membrane protein (VAMP)-associated proteins (VAPs) in an isoform-specific manner. The endoplasmic reticulum (ER) integral membrane protein VAP is a common component of membrane contact sites involved in both tethering and lipid transfer by binding directly to proteins containing an FFAT (two phenylalanines [FF] in an acidic tract [AT]) motif. Sequence analyses revealed that XopM displays two FFAT motifs that cooperatively mediated the interaction of XopM with VAP. When expressed in plants, XopM supported growth of a nonpathogenic bacterial strain and dampened the production of reactive oxygen species, indicating its ability to suppress plant immunity. Further analyses revealed that the interaction with VAP and the ability to suppress microbe-associated molecular pattern-triggered immunity (MTI) are structurally and functionally separable, although XopM requires localisation to the host membrane system for full MTI suppression activity. We discuss a working model in which XopM uses FFAT motifs to target the membrane to interfere with early MTI responses.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 12","pages":"e70038"},"PeriodicalIF":4.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11631713/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ralstonia solanacearum Alters Root Developmental Programmes in Auxin-Dependent and -Independent Manners. 植物生长素依赖性和非依赖性改变根系发育规律。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-12-01 DOI: 10.1111/mpp.70043

Lu Zhang, Gang Yu, Hao Xue, Meng Li, Rosa Lozano-Durán, Alberto P Macho

{"title":"Ralstonia solanacearum Alters Root Developmental Programmes in Auxin-Dependent and -Independent Manners.","authors":"Lu Zhang, Gang Yu, Hao Xue, Meng Li, Rosa Lozano-Durán, Alberto P Macho","doi":"10.1111/mpp.70043","DOIUrl":"10.1111/mpp.70043","url":null,"abstract":"Microbial pathogens and other parasites can modify the development of their hosts, either as a target or a side effect of their virulence activities. The plant-pathogenic bacterium Ralstonia solanacearum, causal agent of the devastating bacterial wilt disease, is a soilborne microbe that invades host plants through their roots and later proliferates in xylem vessels. In this work, we studied the early stages of R. solanacearum infection in the model plant Arabidopsis thaliana, using an in vitro infection system. In addition to the previously reported inhibition of primary root length and increase in root hair formation at the root tip, we observed an earlier xylem differentiation during R. solanacearum infection that occurs in a HrpG-dependent manner, suggesting that the pathogen actively promotes the development of the vascular system upon invasion of the root. Moreover, we found that the phytohormone auxin, of which the accumulation is promoted by the bacterial infection, is required for the R. solanacearum-triggered induction of root hair formation but not earlier xylem differentiation. Altogether, our results shed light on the capacity of R. solanacearum to induce alterations of root developmental pathways and on the role of auxin in this process.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 12","pages":"e70043"},"PeriodicalIF":4.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11662138/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Specific Transcriptional Regulation Controls Plant Organ-Specific Infection by the Oomycete Pathogen Phytophthora sojae. 大豆疫霉卵菌侵染植物器官的特异性转录调控。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-12-01 DOI: 10.1111/mpp.70042

Long Lin, Yang Wang, Hui Qian, Jiawei Wu, Yachun Lin, Yeqiang Xia, Suomeng Dong, Wenwu Ye, Yuanchao Wang

{"title":"Specific Transcriptional Regulation Controls Plant Organ-Specific Infection by the Oomycete Pathogen Phytophthora sojae.","authors":"Long Lin, Yang Wang, Hui Qian, Jiawei Wu, Yachun Lin, Yeqiang Xia, Suomeng Dong, Wenwu Ye, Yuanchao Wang","doi":"10.1111/mpp.70042","DOIUrl":"10.1111/mpp.70042","url":null,"abstract":"The organs of a plant species vary in cell structure, metabolism and defence responses. However, the mechanisms that enable a single pathogen to colonise different plant organs remain unclear. Here we compared the transcriptome of the oomycete pathogen Phytophthora sojae during infection of roots versus leaves of soybeans. We found differences in the transcript levels of hundreds of pathogenicity-related genes, particularly genes encoding carbohydrate-active enzymes, secreted (effector) proteins, oxidoreductase-related proteins and transporters. To identify the key regulator for root-specific infection, we knocked out root-specific transcription factors (TFs) and found the mutants of PsBZPc29, which encodes a member of an oomycete-specific class of basic leucine zipper (bZIP) TFs, displayed reduced virulence on soybean roots but not on leaves. More than 60% of the root-specific genes showed reduced expression in the mutants during root infection. The results suggest that transcriptional regulation underlies the organ-specific infection by P. sojae, and that a bZIP TF plays a key role in root-specific transcriptional regulation.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 12","pages":"e70042"},"PeriodicalIF":4.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11645254/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142822127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: New persistent plant RNA virus carries mutations to weaken viral suppression of antiviral RNA interference. 更正：新的持久性植物RNA病毒携带突变以削弱抗病毒RNA干扰的病毒抑制。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-12-01 DOI: 10.1111/mpp.70035

{"title":"Correction to: New persistent plant RNA virus carries mutations to weaken viral suppression of antiviral RNA interference.","authors":"","doi":"10.1111/mpp.70035","DOIUrl":"10.1111/mpp.70035","url":null,"abstract":"Zhu, L.-J., Zhu, Y., Zou, C., Su, L.-Y., Zhang, C.-T., Wang, C. et al. (2024) New persistent plant RNA virus carries mutations to weaken viral suppression of antiviral RNA interference. Molecular Plant Pathology, 25, e70020. Available from: https://doi.org/10.1111/mpp.70020 The following errors have been identified in the above published article: The co-first authors and co-corresponding authors are not indicated. The order of the funds appearing in the Funding information of the article are not consistent with the order in the Acknowledgements. The authors would like to correct these errors as follows: Li-Juan Zhu and Yu Zhu contributed equally to this work. Jian-Guo Wu and Yan-Hong Han are co-corresponding authors. Jiang-Guo Wu: wujianguo81@126.com; Yan-Hong Han: yan-hong@fafu.edu The correct order of funds is: National Natural Science Foundation of China, Grant/Award Number 32025031 and 31,900,153, National Key Research and Development Program of China, Grant/Award Number 2023YFF1000500 and Special Fund Project for Science and Technology Innovation of FAFU, Grant/Award Number KFB23013. We apologise for these errors.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 12","pages":"e70035"},"PeriodicalIF":4.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11631711/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA Ligase I Circularises Potato Spindle Tuber Viroid RNA in a Biomolecular Condensate. DNA连接酶I在生物分子凝聚物中使马铃薯纺锤形块茎类病毒RNA环状化。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-12-01 DOI: 10.1111/mpp.70047

Yunhan Wang, Junfei Ma, Jie Hao, Bin Liu, Ying Wang

{"title":"DNA Ligase I Circularises Potato Spindle Tuber Viroid RNA in a Biomolecular Condensate.","authors":"Yunhan Wang, Junfei Ma, Jie Hao, Bin Liu, Ying Wang","doi":"10.1111/mpp.70047","DOIUrl":"10.1111/mpp.70047","url":null,"abstract":"Viroids are single-stranded circular noncoding RNAs that mainly infect crops. Upon infection, nuclear-replicating viroids engage host DNA-dependent RNA polymerase II for RNA-templated transcription, which is facilitated by a host protein TFIIIA-7ZF. The sense-strand and minus-strand RNA intermediates are differentially localised to the nucleolus and nucleoplasm regions, respectively. The factors and function underlying the differential localisation of viroid RNAs have not been fully elucidated. The sense-strand RNA intermediates are cleaved into linear monomers by a yet-to-be-identified RNase III-type enzyme and ligated to form circular RNA progeny by DNA ligase I (LIG1). The subcellular compartment for the ligation reaction has not been characterised. Here, we show that LIG1 and potato spindle tuber viroid (PSTVd) colocalise near the nucleolar region in Nicotiana benthamiana protoplasts. The colocalised region is also the highly condensed region of sense-strand PSTVd RNA, indicating that PSTVd RNA and LIG1 form a biomolecular condensate for RNA processing. This finding expands the function of biomolecular condensates to the infection of subviral pathogens. In addition, this knowledge of viroid biogenesis will contribute to exploring thousands of viroid-like RNAs that have been recently identified.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 12","pages":"e70047"},"PeriodicalIF":4.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11665776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to 'Heat Shock Transcription Factor 3 Regulates Plant Immune Response Through Modulation of Salicylic Acid Accumulation and Signalling in Cassava'. 更正“热休克转录因子3通过调节木薯水杨酸积累和信号传导调节植物免疫反应”。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-12-01 DOI: 10.1111/mpp.70032

引用次数: 0

Lrp Family Regulator SCAB_Lrp2 Responds to the Precursor Tryptophan and Represses the Thaxtomin Biosynthesis in Streptomyces scabies. Lrp家族调节因子SCAB_Lrp2响应前体色氨酸并抑制疥疮链霉菌的Thaxtomin生物合成。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-12-01 DOI: 10.1111/mpp.70036

Haoyang He, Lijuan Tang, Mingrui Song, Hui Chen, Youquan Zou, Xueyan Li, Endong Yang, Hang Wu, Buchang Zhang, Jing Liu

{"title":"Lrp Family Regulator SCAB_Lrp2 Responds to the Precursor Tryptophan and Represses the Thaxtomin Biosynthesis in Streptomyces scabies.","authors":"Haoyang He, Lijuan Tang, Mingrui Song, Hui Chen, Youquan Zou, Xueyan Li, Endong Yang, Hang Wu, Buchang Zhang, Jing Liu","doi":"10.1111/mpp.70036","DOIUrl":"https://doi.org/10.1111/mpp.70036","url":null,"abstract":"Streptomyces scabies is a well-researched plant pathogen belonging to the genus Streptomyces. Its virulence is linked to the production of the secondary metabolite thaxtomin A, which is tightly regulated at the transcriptional level. The leucine-responsive regulatory protein (Lrp) family is conserved in prokaryotes and is involved in various crucial biological processes. However, the regulatory interaction between Lrp protein and pathogenic Streptomyces species remains poorly understood. This study aims to explore the role of SCAB_Lrp2 in regulating thaxtomin biosynthesis and pathogenicity, and to analyse the shared and unique features of Lrp homologues in S. scabies. We observed that SCAB_Lrp2 (SCAB_75421) showed significant homology with SCAB_Lrp, a recognised activator of thaxtomin A production in S. scabies. Our results revealed a regulatory interaction between SCAB_Lrp2 and SCAB_Lrp in terms of their targets, although SCAB_Lrp2 does not respond to the amino acid-effectors of SCAB_Lrp. In contrast to SCAB_Lrp, deletion of SCAB_Lrp2 resulted in a notable increase in thaxtomin A production with the emergence of a hypervirulent phenotype in S. scabies. Further analysis revealed that SCAB_Lrp2 represses the transcription of the thaxtomin biosynthetic gene cluster by directly regulating the cluster-situated regulator (CSR) gene txtR. Moreover, the precursor of thaxtomin, tryptophan, acts as an effector of SCAB_Lrp2, strengthening the repressive effect on thaxtomin biosynthesis through txtR. These findings provide new insights into the functional conservation and diversity of Lrp homologues involved in the biosynthesis of thaxtomin phytotoxins in pathogenic Streptomyces species.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 12","pages":"e70036"},"PeriodicalIF":4.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11609053/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142770581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SsPtc3 Modulating SsSmk1-MAPK and Autophagy to Facilitate Growth and Pathogenicity in Sclerotinia sclerotiorum. SsPtc3调控SsSmk1-MAPK和自噬促进菌核菌生长和致病性

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-12-01 DOI: 10.1111/mpp.70037

Wenli Jiao, Dongmeng Ma, Qi Zuo, Yalan Li, Jun Hu, Dongmei Jia, Yanhua Zhang, Jinliang Liu, Xianghui Zhang, Hongyu Pan

{"title":"SsPtc3 Modulating SsSmk1-MAPK and Autophagy to Facilitate Growth and Pathogenicity in Sclerotinia sclerotiorum.","authors":"Wenli Jiao, Dongmeng Ma, Qi Zuo, Yalan Li, Jun Hu, Dongmei Jia, Yanhua Zhang, Jinliang Liu, Xianghui Zhang, Hongyu Pan","doi":"10.1111/mpp.70037","DOIUrl":"https://doi.org/10.1111/mpp.70037","url":null,"abstract":"The compound appressoria of Sclerotinia sclerotiorum can produce cell wall-degrading enzymes, effectors and toxins, which promote penetration and the death of host cells. Subsequently, invasive hyphae (IH) branch rapidly as necrotrophic growth and disease symptoms are observed. S. sclerotiorum can respond to complex stresses and regulate its metabolism to adapt to the external environment. Here we demonstrated that type 2C Ser/Thr phosphatase (PP2C) SsPtc3 responds to nutritional, osmotic, cell wall and oxidative stresses. Loss of function ΔSsptc3 mutants displayed defects in mycelial growth, sclerotia formation and reduced virulence. Phosphoproteomic analyses revealed that SsPtc3 is involved in autophagy and MAPK signalling pathways. We obtained evidence that SsPtc3 negatively modulates the phosphorylation of SsSmk1. SsSmk1 is essential for mycelial growth, compound appressorium formation and pathogenicity, SsPtc3 modulated phosphorylation homeostasis of SsSmk1 to maintain hyphal growth. SsPtc3 interacted with SsAtg1 to influence autophagic flux under starvation. Taken together, these results reveal that SsPtc3 responds to various stresses that modulate autophagy and phosphorylation of SsSmk1-MAPK, which facilitates the growth and virulence of S. sclerotiorum.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 12","pages":"e70037"},"PeriodicalIF":4.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11609052/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142770584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Negative Regulators of the Basal Defence WRKY7, WRKY11 and WRKY17 Modulate the Jasmonic Acid Pathway and an Alternative Splicing Regulatory Network in Response to Pseudomonas syringae in Arabidopsis thaliana. 拟南芥基础防御基因WRKY7、WRKY11和WRKY17的负调控因子调控茉莉酸通路和一个可选剪接调控网络对紫花假单胞菌的响应

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-12-01 DOI: 10.1111/mpp.70044

Isabel Fuenzalida-Valdivia, Ariel Herrera-Vásquez, María Victoria Gangas, Julio Sáez-Vásquez, José Miguel Álvarez, Claudio Meneses, Francisca Blanco-Herrera

{"title":"The Negative Regulators of the Basal Defence WRKY7, WRKY11 and WRKY17 Modulate the Jasmonic Acid Pathway and an Alternative Splicing Regulatory Network in Response to Pseudomonas syringae in Arabidopsis thaliana.","authors":"Isabel Fuenzalida-Valdivia, Ariel Herrera-Vásquez, María Victoria Gangas, Julio Sáez-Vásquez, José Miguel Álvarez, Claudio Meneses, Francisca Blanco-Herrera","doi":"10.1111/mpp.70044","DOIUrl":"10.1111/mpp.70044","url":null,"abstract":"In Arabidopsis thaliana, the transcription factors WRKY7, WRKY11 and WRKY17 act as negative defence regulators against Pseudomonas syringae pv. tomato (Pst) DC3000. However, their coordinated regulation of gene expression has yet to be fully explored. In this study, we conducted a transcriptomic analysis on the triple mutant wrky7/11/17 in response to Pst DC3000 at 0, 3 and 24 h post-inoculation (hpi). Our results suggest that at early infection stages (0 and 3 hpi), WRKY7, WRKY11 and WRKY17 significantly repress a group of genes involved in signal perception and transduction, including receptor-like kinases. Furthermore, at later stages of interaction (24 hpi), these transcription factors induce genes related to the biosynthesis and signalling of the jasmonic acid (JA) pathway. Further infection experiments with Pst DC3000 in plants treated with methyl jasmonate (a JA analogue) and infections with Botrytis cinerea, a pathogen against which JA-mediated responses are crucial for effective defence, support this proposal. Moreover, we analysed the role of WRKY7, WRKY11 and WRKY17 in alternative splicing regulation. A comparison between differentially expressed (DEG) and spliced (DAS) genes revealed that over 80% of DAS events do not occur in conjunction with overall changes in gene expression. Alternative splicing events were found in genes with functions in splicing and the JA pathway, such as ALY4, PRP40A, JAZ3 and JAZ10. These results suggest that WRKY7, WRKY11 and WRKY17 can also participate in this layer of gene expression regulation to modulate immunity negatively.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 12","pages":"e70044"},"PeriodicalIF":4.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11667101/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytokinin Plays a Multifaceted Role in Ralstonia solanacearum-Triggered Plant Disease Development. 细胞分裂素在植物病害发展中起着多方面的作用。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-12-01 DOI: 10.1111/mpp.70045

Xiang Wang, Qichang Gong, Shengyang Cheng, Ning Qin, Tao Cao, Yue Chen, Dongdong Wang, Marc Valls, Núria S Coll, Qin Chen, Cuizhu Zhao, Haibin Lu

{"title":"Cytokinin Plays a Multifaceted Role in Ralstonia solanacearum-Triggered Plant Disease Development.","authors":"Xiang Wang, Qichang Gong, Shengyang Cheng, Ning Qin, Tao Cao, Yue Chen, Dongdong Wang, Marc Valls, Núria S Coll, Qin Chen, Cuizhu Zhao, Haibin Lu","doi":"10.1111/mpp.70045","DOIUrl":"10.1111/mpp.70045","url":null,"abstract":"Cytokinin signalling plays both positive and negative roles in plant resistance to pathogens. It is not clear whether the role of cytokinin changes at the different stages of pathogen infection. Arabidopsis thaliana sequentially exhibits distinct root morphological symptoms during Ralstonia solanacearum infection, which offers a good system to investigate function of cytokinin in the whole pathogen infection process. Using this system, we found increase of cytokinin signalling by Lonely Guy 2 (LOG2) overexpression or depletion of type-A Arabidopsis Response Regulators (ARRs), negative regulators of cytokinin signalling pathway, promoted cell death, wilting symptom and bacterial growth, but attenuated primary root growth inhibition and lateral root formation. The decrease of cytokinin signalling by mutation on Isopentenyl Transferases (IPTs) inhibited root hair formation, cell death, wilting symptom and bacterial colonisation. Application of different concentration of exogenesis 6-benzylaminopurine (6-BA) showed first promoted, then decreased root hair formation. Moreover, application of 6-BA accelerated cell death but suppressed lateral root formation and primary root growth inhibition. The diverse roles of cytokinin in these different root disease phenotypes suggested function of cytokinin during plant responses to R. solanacearum is cell type-specific, which provides new insights on roles of cytokinin signalling in regulation on plant-pathogen interactions.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 12","pages":"e70045"},"PeriodicalIF":4.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11671236/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0