Molecular plant pathology最新文献_第3页

Engineered Biomolecular Condensates Limit Tobacco Mosaic Virus Accumulation and Symptom Development. 工程生物分子凝聚物限制烟草花叶病毒的积累和症状发展。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-06-01 DOI: 10.1111/mpp.70113

Alyssa M Stanfield, Jared P May

{"title":"Engineered Biomolecular Condensates Limit Tobacco Mosaic Virus Accumulation and Symptom Development.","authors":"Alyssa M Stanfield, Jared P May","doi":"10.1111/mpp.70113","DOIUrl":"https://doi.org/10.1111/mpp.70113","url":null,"abstract":"Artificial condensates formed by liquid-liquid phase separation (LLPS) have been extensively studied in the field of synthetic biology for their ability to control cellular functions in a reversible and tunable manner. Although cellular condensates can restrict viral accumulation, the potential to employ LLPS as a platform for targeted antiviral strategies remains understudied. Here, we engineered tobacco mosaic virus (TMV) to harbour MS2 bacteriophage hairpins that are specifically bound by the MS2 coat protein (MCP). Using well-studied intrinsically disordered regions (IDRs) as scaffolds, we engineered artificial condensates that could target MS2-tagged TMV, inhibiting TMV accumulation up to fivefold following transient expression in Nicotiana benthamiana. TAR DNA-binding protein 43 (TDP-43)-based condensates inhibited MS2-tagged virus accumulation by over twofold, independent of the global translational repression observed during condensate expression. Following manual inoculation of MS2-tagged TMV onto leaves transiently expressing TDP-43:MCP condensates, systemic virus accumulation was reduced by over 10-fold, which was associated with a significant decrease in symptom severity. These results provide a foundation for developing artificial biomolecular condensates to mitigate plant virus accumulation and disease severity.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 6","pages":"e70113"},"PeriodicalIF":4.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12182602/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Aquaporin MdPIP2;2 Regulates Glomerella Leaf Spot Resistance in Apple. 水通道蛋白MdPIP2；2调控苹果肾小球叶斑病抗性。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-06-01 DOI: 10.1111/mpp.70111

Yubo Sun, Yufei Zhou, Junquan Chen, Xiaochun Ma, Xiaoqing Gong, Jie Yang, Changhai Liu, Fengwang Ma

引用次数: 0

A Lrp/AsnC Family Transcriptional Regulator Lrp Is Essential for the Pathogenicity of Dickeya oryzae. Lrp/AsnC家族转录调控因子Lrp对稻瘟病菌致病性的影响

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-06-01 DOI: 10.1111/mpp.70100

Xiaoyan Wu, Qunyi Chen, Huidi Liu, Weihan Gu, Yizhen Deng, Lian-Hui Zhang, Zhibin Liang

{"title":"A Lrp/AsnC Family Transcriptional Regulator Lrp Is Essential for the Pathogenicity of Dickeya oryzae.","authors":"Xiaoyan Wu, Qunyi Chen, Huidi Liu, Weihan Gu, Yizhen Deng, Lian-Hui Zhang, Zhibin Liang","doi":"10.1111/mpp.70100","DOIUrl":"10.1111/mpp.70100","url":null,"abstract":"Dickeya oryzae causes severe soft rot diseases in a range of important crops. To understand its complicated pathogenic mechanisms, we tried to identify the key virulence regulators through transposon mutagenesis. This led to the identification of a member of the Lrp/AsnC family transcriptional regulators in D. oryzae EC1, designated as Lrp. Phenotype analyses showed that Lrp positively regulated biofilm formation and the production of zeamines, proteases and polygalacturonases, but negatively regulated bacterial swimming motility. Deletion of lrp caused a drastic attenuation in bacterial virulence, indicating that Lrp is a key regulator in the modulation of D. oryzae pathogenicity. We further showed that the transcription of the lrp gene was negatively regulated by the transcriptional regulators SlyA, Fis and OhrR, and the transcriptional expression of tzpA, ohrR and fis was positively modulated by Lrp. Moreover, we demonstrated that Lrp can directly bind to the promoter regions of zmsA, zmsK, prtG, prtX, pehK, pehX, fis, tzpA and ohrR. DNase I footprinting assay determined that Lrp was capable of binding to a specific site (5'-GTGTAATTATGGGCGTGCTCCGGG-3') in the promoter of zmsA. Furthermore, we found that four amino acid residues of Lrp, L20, L23, G111 and T146, are essential to the biological function of Lrp. Overall, this study demonstrated that Lrp is an essential virulence modulator in D. oryzae and suggested that Lrp can be a potent target for controlling the soft rot diseases caused by D. oryzae.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 6","pages":"e70100"},"PeriodicalIF":4.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145271/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Tetracycline-Inducible/CRISPR-Cas9 System is an Efficient Tool for Studying Gene Function in Phytophthora sojae. 四环素诱导/CRISPR-Cas9系统是研究大豆疫霉基因功能的有效工具

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-06-01 DOI: 10.1111/mpp.70114

Chengcheng Li, Xiaofei Liu, Yiying Li, Qin Peng, Jianqiang Miao, Xili Liu

{"title":"The Tetracycline-Inducible/CRISPR-Cas9 System is an Efficient Tool for Studying Gene Function in Phytophthora sojae.","authors":"Chengcheng Li, Xiaofei Liu, Yiying Li, Qin Peng, Jianqiang Miao, Xili Liu","doi":"10.1111/mpp.70114","DOIUrl":"10.1111/mpp.70114","url":null,"abstract":"The present study presents a novel approach combining a tetracycline-inducible system (Tet-On) and CRISPR-Cas9 techniques to investigate the function of two essential genes in Phytophthora sojae. We constructed a donor vector in which the reverse tetracycline transactivator (rtTA) is driven by an oomycete promoter. Additionally, it contains a fused TetR binding site and the minimum oomycete promoter, as well as 1000-bp homologous arms of the promoter upstream and downstream sequences. The promoter of the target gene was replaced with a tetracycline-responsive promoter (Ptet) using a CRISPR-Cas9 system. In the native transformants, the target gene was induced by the administration of tetracycline and repressed in its absence. Using the Tet-On/CRISPR-Cas9 system, we obtained inducible transformants of PsAF5 and PsCesA3. The phenotype of PsAF5 inducible transformants without doxycycline was consistent with that of ΔPsAF5 transformants, specifically characterised by an increase in oospore production and heightened sensitivity to H2O2. PsCesA3 inducible transformants could not grow in the absence of doxycycline, which means PsCesA3 is an essential protein for P. sojae. In conclusion, the Tet-On/CRISPR-Cas9 system represents an effective approach for investigating crucial genes in P. sojae.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 6","pages":"e70114"},"PeriodicalIF":4.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186864/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NbHDR, A Host Protein Involved in the MEP Pathway, Interacts With Bamboo Mosaic Virus Replicase and Enhances Viral Accumulation. 参与MEP途径的宿主蛋白NbHDR与竹花叶病毒复制酶相互作用并促进病毒积累

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-06-01 DOI: 10.1111/mpp.70099

Chi Hzeng Wong, Chung-Chi Hu, Ching-Hsiu Tsai, Na-Sheng Lin, Yau-Heiu Hsu, Ming-Kuem Lin, Ying-Wen Huang

{"title":"NbHDR, A Host Protein Involved in the MEP Pathway, Interacts With Bamboo Mosaic Virus Replicase and Enhances Viral Accumulation.","authors":"Chi Hzeng Wong, Chung-Chi Hu, Ching-Hsiu Tsai, Na-Sheng Lin, Yau-Heiu Hsu, Ming-Kuem Lin, Ying-Wen Huang","doi":"10.1111/mpp.70099","DOIUrl":"10.1111/mpp.70099","url":null,"abstract":"Plant viruses, as obligate parasites, depend on host cellular machinery for various processes essential to their life cycle, making the investigation of these interactions fundamentally important. Bamboo mosaic virus (BaMV), a positive-strand (+) RNA virus, serves as a model to explore host-virus interactions during replication. In this study, Nicotiana benthamiana 1-hydroxy-2-methyl-butenyl 4-diphosphate reductase (NbHDR), a key enzyme in the methylerythritol 4-phosphate (MEP) pathway, was identified as an interactor with BaMV replicase through immunoprecipitation, pull-down and yeast two-hybrid assays. Knockdown of NbHDR significantly reduced the accumulation of BaMV RNA and coat protein but did not affect infection with the close relative potato virus X, indicating its specific involvement in BaMV replication. Overexpression of NbHDR in N. benthamiana or the addition of NbHDR in in vitro RdRp reactions demonstrated that NbHDR enhances BaMV replication by promoting (+) RNA synthesis. To further explore whether the role of NbHDR in BaMV replication is linked to gibberellic acid (GA) synthesis through the MEP pathway, individual knockdowns of NbHDR and ent-kaurene synthase (KS), a key enzyme in GA biosynthesis, were performed. Silencing KS sireduced BaMV accumulation, which was rescued by exogenous GA, but GA supplementation was insufficient to restore BaMV levels after NbHDR silencing. These findings suggest that NbHDR associates with the BaMV replication complex to enhance viral replication efficiency through a mechanism independent of GA synthesis from the MEP pathway, providing new insights into host-virus interactions.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 6","pages":"e70099"},"PeriodicalIF":4.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186863/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Apoplast Metabolomics Profiling Reveals Nitrogen-Dependent Modulation of Plant-Pathogen Interactions. 外质体代谢组学分析揭示植物与病原体相互作用的氮依赖调节。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-06-01 DOI: 10.1111/mpp.70110

Roua Jeridi, Antoine Davière, Sylvie Jolivet, Marco Zarattini, Gilles Clement, Marie-Christine Soulie, Ahmed Landoulsi, Mathilde Fagard

{"title":"Apoplast Metabolomics Profiling Reveals Nitrogen-Dependent Modulation of Plant-Pathogen Interactions.","authors":"Roua Jeridi, Antoine Davière, Sylvie Jolivet, Marco Zarattini, Gilles Clement, Marie-Christine Soulie, Ahmed Landoulsi, Mathilde Fagard","doi":"10.1111/mpp.70110","DOIUrl":"10.1111/mpp.70110","url":null,"abstract":"In the present study, we analysed the role played by the apoplast in the crosstalk between biotic and abiotic stress conditions. In particular, we studied the crosstalk between nitrogen (N) limitation and infection of the model plant Arabidopsis thaliana by Erwinia amylovora, an apoplastic bacterium. Our previous findings indicated that low N (LN) conditions increase E. amylovora in planta titres and expression of virulence factors. In this work, we extracted the apoplast wash fluids (AWF) from plants grown under low N or high N (HN) conditions and applied them to bacteria in vitro. We observed that LN-AWF induced stronger virulence gene expression than HN-AWF. Metabolomic analysis of both apoplast extracts revealed the presence of common metabolites; however, their proportions were distinct, indicating a direct effect of N availability on apoplast content. Interestingly, changes in the apoplast metabolite proportions were also observed early after bacterial infection, but only in plants grown under LN conditions. To evaluate the effect of single metabolites on virulence gene expression, we selected 43 metabolites and observed that 29 of them were activators, whereas two, GABA and citrate, acted as repressors. This study shows that environmental constraints, such as N availability, impact plant-pathogen interactions by altering the apoplastic content.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 6","pages":"e70110"},"PeriodicalIF":4.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12190549/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144497505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wheat TaNADPO Promotes Spot Blotch Resistance. 小麦TaNADPO提高抗斑点病能力。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-06-01 DOI: 10.1111/mpp.70103

Meng Yuan, Qingdong Zeng, Lei Hua, Shisheng Chen, Jianhui Wu, Shuqing Zhao, Mengyu Li, Xiaopeng Ren, Linfei Ma, Zihan Liu, Kaixuan Wang, Manli Sun, Hongfei Yan, Zhensheng Kang, Dejun Han, Xiaodong Wang

{"title":"Wheat TaNADPO Promotes Spot Blotch Resistance.","authors":"Meng Yuan, Qingdong Zeng, Lei Hua, Shisheng Chen, Jianhui Wu, Shuqing Zhao, Mengyu Li, Xiaopeng Ren, Linfei Ma, Zihan Liu, Kaixuan Wang, Manli Sun, Hongfei Yan, Zhensheng Kang, Dejun Han, Xiaodong Wang","doi":"10.1111/mpp.70103","DOIUrl":"10.1111/mpp.70103","url":null,"abstract":"Bipolaris sorokiniana is a prevalent fungal pathogen that resides in the soil and affects various parts of wheat, leading to diseases such as spot blotch, common root rot, head blight and black point. The genetic mechanisms that confer resistance in wheat against this pathogen are not completely known. In this research, 1302 wheat germplasms from around the world were evaluated for resistance to spot blotch at the seedling stage, and it was found that merely 3.8% displayed moderate or better resistance. A genome-wide association study (GWAS) employing high-density 660K single-nucleotide polymorphism (SNP) data pinpointed a segment on chromosome 1BL (621.2-674.0 Mb) containing nine SNPs that are significantly linked to spot blotch resistance, named Qsb.hebau-1BL. RNA sequencing and reverse transcription-quantitative PCR analyses demonstrated that the gene TraesCS1B02G410300, which codes for nicotinamide-adenine dinucleotide phosphate-binding oxidoreductase (TaNADPO), was markedly upregulated by B. sorokiniana. Five SNP variations were identified in the promoter region of TaNADPO in wheat lines with or without Qsb.hebau-1BL. Wheat lines that overexpressed TaNADPO exhibited increased resistance to spot blotch and higher accumulation of reactive oxygen species (ROS). In contrast, knockout EMS mutants of Triticum turgidum TdNADPO (tdnadpo-K2561, Gln125*) and TaNADPO (tanadpo-J10516796, splice donor variant) showed diminished resistance and lower ROS levels. In conclusion, TaNADPO is a key gene for resistance against B. sorokiniana, providing essential information for the development of spot blotch-resistant wheat varieties through molecular breeding techniques.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 6","pages":"e70103"},"PeriodicalIF":4.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145272/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HrpW Modulates Paracidovorax citrulli Virulence and Plant Immunity via ClRAR1 Interaction in Watermelon. HrpW通过ClRAR1互作调节瓜副酸虫病毒力和植物免疫

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-06-01 DOI: 10.1111/mpp.70108

Tong Qin, Xiuan Liang, Chen Zhong, Zhiheng Zhang, Jingjue Wang, Jiahuan Shi, Jingjing Huang, Dong Chen, Wei Zhao, Mengyang Wang, Jianlong Zhao, Yongqiang He, Shanshan Yang, Ali Chai, Xiaoxiao Zhang

{"title":"HrpW Modulates Paracidovorax citrulli Virulence and Plant Immunity via ClRAR1 Interaction in Watermelon.","authors":"Tong Qin, Xiuan Liang, Chen Zhong, Zhiheng Zhang, Jingjue Wang, Jiahuan Shi, Jingjing Huang, Dong Chen, Wei Zhao, Mengyang Wang, Jianlong Zhao, Yongqiang He, Shanshan Yang, Ali Chai, Xiaoxiao Zhang","doi":"10.1111/mpp.70108","DOIUrl":"10.1111/mpp.70108","url":null,"abstract":"Bacterial fruit blotch (BFB), caused by Paracidovorax citrulli, severely threatens watermelon production. This study investigates the role of HrpW, an atypical harpin in P. citrulli AAC00-1, in bacterial virulence and host immune modulation. Bioinformatics analysis revealed HrpW harbours a unique signal peptide and structural features distinct from other harpins. Deletion of hrpW impaired bacterial motility, biofilm formation and virulence, while complementation restored these traits. HrpW suppressed reactive oxygen species (ROS) bursts and mitogen-activated protein kinase (MAPK) activation in plants but failed to induce programmed cell death (PCD). Crucially, HrpW inhibited the hypersensitive response (HR) triggered by P. citrulli in non-host tobacco, with ΔhrpW mutant inducing premature HR. RNA-seq analysis demonstrated HrpW downregulated ClRAR1 expression. Silencing ClRAR1 or ClSGT1 compromised watermelon resistance, and notably, ClRAR1-silenced plants exhibited PCD upon HrpW treatment, indicating HrpW-mediated PCD suppression is RAR1-dependent. Importantly, HrpW triggered ubiquitin-dependent degradation of ClRAR1 and independently disrupted ClRAR1-ClSGT1 binding, thereby suppressing effector-triggered immunity (ETI). HrpW translocated into plant cells via the type III secretion system (T3SS), as confirmed by CyaA assays. Intriguingly, low concentrations of HrpW enhanced watermelon resistance to BFB, while high concentrations promoted disease progression, revealing a concentration-dependent duality. This study unveils HrpW as a multifunctional virulence factor that modulates bacterial fitness, suppresses HR and manipulates host immunity via RAR1 targeting. These findings expand our understanding of harpin-mediated pathogenicity and offer insights for sustainable BFB management strategies.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 6","pages":"e70108"},"PeriodicalIF":4.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170954/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ghr-miR166b-GhHB14 Pair With GhDLO1 Partner Mediates Cotton Plant Defence Against Verticillium dahliae. Ghr-miR166b-GhHB14对GhDLO1伴侣介导棉花植株对大丽花黄萎病的防御

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-06-01 DOI: 10.1111/mpp.70104

Yuan Meng, Ge Zhao, Ye Tang, Lin Xu, Dongliang Li, Xiaoyang Ge, Jiahe Wu

{"title":"Ghr-miR166b-GhHB14 Pair With GhDLO1 Partner Mediates Cotton Plant Defence Against Verticillium dahliae.","authors":"Yuan Meng, Ge Zhao, Ye Tang, Lin Xu, Dongliang Li, Xiaoyang Ge, Jiahe Wu","doi":"10.1111/mpp.70104","DOIUrl":"10.1111/mpp.70104","url":null,"abstract":"In plants, the class III homeodomain-leucine zipper (HD-ZIP III) transcription factors regulate a broad range of developmental processes. However, less research on HD-ZIP IIIs has been done on Verticillium wilt, which is a devastating disease of cotton production worldwide. Here, we report that a cotton HD-ZIP III (GhHB14) targeted by ghr-miR166b participates in plant resistance to Verticillium wilt. According to degradome data, RACE sequencing and in vivo fluorescent light assay, ghr-miR166b can target GhHB14 mRNA to directly cleave it at nucleotide position 586, suggesting that GhHB14 expression is mediated by a post-transcriptional process. Yeast two-hybrid and bimolecular fluorescence complementation analyses showed that GhHB14 can interact with GhDLO1 in cells. Plants silenced for ghr-miR166b showed significantly higher susceptibility to Verticillium dahliae infection compared to the control, while GhHB14- and GhDLO1-silenced plants exhibited significantly higher resistance. These results suggested that ghr-miR166b is a positive regulator in plant resistance against Verticillium wilt, whereas GhHB14 and GhDLO1 are both negative regulators. Therefore, ghr-miR166b-GhHB14 coupled with GhDLO1 regulates cotton plant defence against V. dahliae.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 6","pages":"e70104"},"PeriodicalIF":4.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12151801/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144266739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systemic and Phloem-Specific Protein Targeting by High Affinity Nanobodies Expressed From a Plant RNA Virus Vector. 植物RNA病毒载体表达的高亲和力纳米体对系统和韧皮部特异性蛋白的靶向作用。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-06-01 DOI: 10.1111/mpp.70105

Angel Y S Chen, Giada Spigolon, Lorenzo Scipioni, James C K Ng

{"title":"Systemic and Phloem-Specific Protein Targeting by High Affinity Nanobodies Expressed From a Plant RNA Virus Vector.","authors":"Angel Y S Chen, Giada Spigolon, Lorenzo Scipioni, James C K Ng","doi":"10.1111/mpp.70105","DOIUrl":"10.1111/mpp.70105","url":null,"abstract":"The emergence of nanobodies (Nbs) has kindled an avid interest for their use in genetic engineering and plant biotechnology. In planta expression of Nbs has relied on either stable or transient transformation approaches that are lengthy and cannot support systemic expression, respectively. In addition, there is no precedence for studies on tissue-specific expression of Nbs. To address these issues, viral vectors could be used as an alternative, but this has not been shown. Here, this proof-of-concept study establishes a platform to demonstrate the phloem-specific targeting of proteins by Nbs expressed from a citrus tristeza virus-based vector. The vector facilitates anti-green fluorescent protein (GFP) Nb production within the phloem of transgenic Nicotiana benthamiana plants expressing a GFP-fused endoplasmic reticulum-targeting peptide and that of a microtubule marker line expressing GFP-fused α-tubulin 6. The interaction between anti-GFP Nb and the GFP-tagged peptide/protein is corroborated by both pull-down assays and fluorescence resonance energy transfer-fluorescence lifetime imaging microscopy (FRET-FLIM) measurements. This proof-of-concept platform-including validation of Nb-antigen interaction in the phloem by FRET-FLIM analysis, which has not been described in the literature-is novel for exploring Nb-mediated functions applicable to targeting or identifying phloem proteins and those co-opted into the virus infection process.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 6","pages":"e70105"},"PeriodicalIF":4.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12166124/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144294117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0