Yuan Meng, Ge Zhao, Ye Tang, Lin Xu, Dongliang Li, Xiaoyang Ge, Jiahe Wu
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引用次数: 0
摘要
在植物中，III类同源结构域亮氨酸拉链（HD-ZIP III）转录因子调控着广泛的发育过程。然而，HD-ZIP iii对黄萎病的研究较少，黄萎病是世界范围内棉花生产的毁灭性疾病。在这里，我们报道了ghr-miR166b靶向的棉花HD-ZIP III （GhHB14）参与了植物对黄萎病的抗性。根据降解组数据、RACE测序和体内荧光测定，ghr-miR166b可以靶向GhHB14 mRNA，直接在586核苷酸位置切割GhHB14 mRNA，表明GhHB14的表达是通过转录后过程介导的。酵母双杂交和双分子荧光互补分析表明，GhHB14可以与细胞中的GhDLO1相互作用。ghr-miR166b沉默植株对大丽花黄萎病的敏感性显著高于对照，而GhHB14-和ghdlo1沉默植株对黄萎病的抗性显著高于对照。这些结果表明ghr-miR166b是植物抗黄萎病的正调控因子，而GhHB14和GhDLO1都是负调控因子。因此，ghr-miR166b-GhHB14与GhDLO1偶联可调控棉花植株对大丽花病菌的防御。
Ghr-miR166b-GhHB14 Pair With GhDLO1 Partner Mediates Cotton Plant Defence Against Verticillium dahliae.
In plants, the class III homeodomain-leucine zipper (HD-ZIP III) transcription factors regulate a broad range of developmental processes. However, less research on HD-ZIP IIIs has been done on Verticillium wilt, which is a devastating disease of cotton production worldwide. Here, we report that a cotton HD-ZIP III (GhHB14) targeted by ghr-miR166b participates in plant resistance to Verticillium wilt. According to degradome data, RACE sequencing and in vivo fluorescent light assay, ghr-miR166b can target GhHB14 mRNA to directly cleave it at nucleotide position 586, suggesting that GhHB14 expression is mediated by a post-transcriptional process. Yeast two-hybrid and bimolecular fluorescence complementation analyses showed that GhHB14 can interact with GhDLO1 in cells. Plants silenced for ghr-miR166b showed significantly higher susceptibility to Verticillium dahliae infection compared to the control, while GhHB14- and GhDLO1-silenced plants exhibited significantly higher resistance. These results suggested that ghr-miR166b is a positive regulator in plant resistance against Verticillium wilt, whereas GhHB14 and GhDLO1 are both negative regulators. Therefore, ghr-miR166b-GhHB14 coupled with GhDLO1 regulates cotton plant defence against V. dahliae.
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