Ghr-miR166b-GhHB14 Pair With GhDLO1 Partner Mediates Cotton Plant Defence Against Verticillium dahliae.

IF 4.8 1区农林科学 Q1 PLANT SCIENCES

Molecular plant pathology Pub Date : 2025-06-01 DOI:10.1111/mpp.70104

Yuan Meng, Ge Zhao, Ye Tang, Lin Xu, Dongliang Li, Xiaoyang Ge, Jiahe Wu

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引用次数: 0

Abstract

In plants, the class III homeodomain-leucine zipper (HD-ZIP III) transcription factors regulate a broad range of developmental processes. However, less research on HD-ZIP IIIs has been done on Verticillium wilt, which is a devastating disease of cotton production worldwide. Here, we report that a cotton HD-ZIP III (GhHB14) targeted by ghr-miR166b participates in plant resistance to Verticillium wilt. According to degradome data, RACE sequencing and in vivo fluorescent light assay, ghr-miR166b can target GhHB14 mRNA to directly cleave it at nucleotide position 586, suggesting that GhHB14 expression is mediated by a post-transcriptional process. Yeast two-hybrid and bimolecular fluorescence complementation analyses showed that GhHB14 can interact with GhDLO1 in cells. Plants silenced for ghr-miR166b showed significantly higher susceptibility to Verticillium dahliae infection compared to the control, while GhHB14- and GhDLO1-silenced plants exhibited significantly higher resistance. These results suggested that ghr-miR166b is a positive regulator in plant resistance against Verticillium wilt, whereas GhHB14 and GhDLO1 are both negative regulators. Therefore, ghr-miR166b-GhHB14 coupled with GhDLO1 regulates cotton plant defence against V. dahliae.

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Ghr-miR166b-GhHB14对GhDLO1伴侣介导棉花植株对大丽花黄萎病的防御

在植物中，III类同源结构域亮氨酸拉链（HD-ZIP III）转录因子调控着广泛的发育过程。然而，HD-ZIP iii对黄萎病的研究较少，黄萎病是世界范围内棉花生产的毁灭性疾病。在这里，我们报道了ghr-miR166b靶向的棉花HD-ZIP III （GhHB14）参与了植物对黄萎病的抗性。根据降解组数据、RACE测序和体内荧光测定，ghr-miR166b可以靶向GhHB14 mRNA，直接在586核苷酸位置切割GhHB14 mRNA，表明GhHB14的表达是通过转录后过程介导的。酵母双杂交和双分子荧光互补分析表明，GhHB14可以与细胞中的GhDLO1相互作用。ghr-miR166b沉默植株对大丽花黄萎病的敏感性显著高于对照，而GhHB14-和ghdlo1沉默植株对黄萎病的抗性显著高于对照。这些结果表明ghr-miR166b是植物抗黄萎病的正调控因子，而GhHB14和GhDLO1都是负调控因子。因此，ghr-miR166b-GhHB14与GhDLO1偶联可调控棉花植株对大丽花病菌的防御。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular plant pathology 生物-植物科学

CiteScore

9.40

自引率

4.10%

发文量

120

审稿时长

6-12 weeks

期刊介绍： Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.