两个pd相关蛋白的功能缺失赋予水稻条纹病毒抗性。

IF 4.8 1区 农林科学 Q1 PLANT SCIENCES
Hong Lu, Panpan Ge, Yonghuan Ma, Feng Lin, Dedao Jing, Tong Zhou, Feng Cui
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引用次数: 0

摘要

植物病毒通常利用胞间连丝(plasmodesmata, pd)感染寄主植物。虽然PD相关蛋白通常参与PD孔径的调节,但少数有限的病例表明它们作为适合抗性育种的病毒靶点的作用。本研究筛选水稻进口蛋白α家族,鉴定pd相关成员,并探讨其对水稻条纹病毒(RSV)感染的影响。输入蛋白α1b和α4均定位于质膜和PD上。只有输入蛋白α4敲除突变体水稻对RSV感染表现出抗性,而输入蛋白α1b在RSV感染中的作用可以忽略不计。缺乏输入蛋白α4增强了pd处胼胝质沉积,从而阻碍了病毒的细胞间运动。Flotillin 1是水稻中另一种pd相关蛋白,以前曾报道过促进呼吸道合胞病毒感染。当同时敲除flotillin1和importin α4时,该双敲除突变体不仅在温室中,而且在不影响农艺性状的情况下,对RSV表现出更高的协同抗性。本研究提出了两种pd相关蛋白在未来作为工程病毒抗性靶点的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Loss-of-Function of Two PD-Associated Proteins Confers Resistance to Rice Stripe Virus.

Plant viruses usually exploit plasmodesmata (PDs) to achieve cellular infection in host plants. Although PD-associated proteins are commonly implicated in the regulation of PD pore size, a few limited cases demonstrate their roles as viral targets suitable for resistance breeding. Here we screened the importin α protein family of rice to identify the PD-associated members and explored their effects on the infection of rice stripe virus (RSV), one of the most notorious pathogens threatening rice yields. Both Importin α1b and α4 were found to be localised on the plasma membrane and PD. Only importin α4 knockout mutant rice exhibited resistance to RSV infection while the role of importin α1b in RSV infection was negligible. The absence of importin α4 enhanced callose deposition at PDs, which impeded viral intercellular movement. Flotillin 1 is another PD-associated protein in rice and was previously reported to facilitate RSV infection. When flotillin1 and importin α4 were simultaneously knocked out, the double-knockout mutant exhibited a synergically higher resistance level to RSV not only in the greenhouse but also in natural fields without affecting agronomic traits. This study proposed the potential of the two PD-associated proteins as targets for engineering virus resistance in future.

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来源期刊
Molecular plant pathology
Molecular plant pathology 生物-植物科学
CiteScore
9.40
自引率
4.10%
发文量
120
审稿时长
6-12 weeks
期刊介绍: Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.
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