Transcription Activator FgDDT Interacts With FgISW1 to Regulate Fungal Development and Pathogenicity in the Global Pathogen Fusarium graminearum.

Abstract

Several DNA-binding homeobox and different transcription factor (DDT)-domain proteins form stable remodelling complexes with imitation switch (ISWI) chromatin remodelling factors. ISWI complexes have been reported to be involved in various biological processes in many eukaryotic species. However, in phytopathogenic fungi, the regulatory mechanisms underlying the functions of DDT-domain proteins in ISWI complexes remain unclear. Here, chromatin immunoprecipitation-sequencing (ChIP-seq) assays were used to demonstrate that FgDDT from Fusarium graminearum was enriched within the promoter regions of genes associated with metabolic and MAPK signalling pathways, thereby activating their expression. Moreover, two additional ISWI genes, FgISW1 and FgISW2, were identified and characterised, with subsequent analyses indicating that the ISWI components FgISW1 and FgDDT are essential for fungal development and pathogenicity rather than FgISW2. Further experiments revealed that FgDDT binds to FgISW1 to form an ISWI complex that activates the expression of functional genes in F. graminearum, consequently contributing to its pathogenicity and development. FgDDT was also observed as highly conserved in Fusarium species but exhibits low similarity to homologues in Homo sapiens and Arabidopsis thaliana, suggesting that functional studies of FgDDT are crucial to uncover its unique role within Fusarium. These findings provide a basis for further understanding the molecular mechanisms by which ISWI complexes function in fungi and contribute to their pathogenicity.