Guangxing Miao, Jiatao Xie, Yanping Fu, Bo Li, Tao Chen, Yang Lin, Xiao Yu, Tom Hsiang, Daohong Jiang, Jiasen Cheng
{"title":"A Necrotrophic Phytopathogen-Derived GPI-Anchored Protein Functions as an Elicitor to Activate Plant Immunity and Enhance Resistance.","authors":"Guangxing Miao, Jiatao Xie, Yanping Fu, Bo Li, Tao Chen, Yang Lin, Xiao Yu, Tom Hsiang, Daohong Jiang, Jiasen Cheng","doi":"10.1111/mpp.70072","DOIUrl":null,"url":null,"abstract":"<p><p>GPI-anchored proteins are widely distributed in eukaryotic cells. However, their functions are still poorly understood in necrotrophic pathogenic fungi. Here, based on Agrobacterium tumefaciens-mediated transient expression screening, a novel secreted GPI-anchored protein, SsGP1, that induces plant cell death was characterised in Sclerotinia sclerotiorum. The homologues of SsGP1 are broadly distributed among ascomycetes. SsGP1 can activate plant immune responses, including reactive oxygen species (ROS) burst and the up-regulated expression of immunity genes, in a manner that is dependent on BAK1 but independent of SOBIR1. Treatment of plants with SsGP1 protein enhanced the resistance of Nicotiana benthamiana and Arabidopsis thaliana to S. sclerotiorum. Our findings reveal that SsGP1 functions as a pathogen-associated molecular pattern (PAMP) and is recognised by plants in a BAK1-dependent manner.</p>","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 4","pages":"e70072"},"PeriodicalIF":4.8000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950629/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular plant pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1111/mpp.70072","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
GPI-anchored proteins are widely distributed in eukaryotic cells. However, their functions are still poorly understood in necrotrophic pathogenic fungi. Here, based on Agrobacterium tumefaciens-mediated transient expression screening, a novel secreted GPI-anchored protein, SsGP1, that induces plant cell death was characterised in Sclerotinia sclerotiorum. The homologues of SsGP1 are broadly distributed among ascomycetes. SsGP1 can activate plant immune responses, including reactive oxygen species (ROS) burst and the up-regulated expression of immunity genes, in a manner that is dependent on BAK1 but independent of SOBIR1. Treatment of plants with SsGP1 protein enhanced the resistance of Nicotiana benthamiana and Arabidopsis thaliana to S. sclerotiorum. Our findings reveal that SsGP1 functions as a pathogen-associated molecular pattern (PAMP) and is recognised by plants in a BAK1-dependent manner.
期刊介绍：
Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
