Xiaoxiao Xie, Jian Ling, Shaoyun Dong, Mingjuan Zhai, Junru Lu, Jianlong Zhao, Xueyong Yang, Xin Dong, Yan Li, Richard G F Visser, Yuling Bai, Zhenchuan Mao, Shengping Zhang, Bingyan Xie
{"title":"Clade V MLO Genes Are Negative Modulators of Cucumber Defence Response to Meloidogyne incognita.","authors":"Xiaoxiao Xie, Jian Ling, Shaoyun Dong, Mingjuan Zhai, Junru Lu, Jianlong Zhao, Xueyong Yang, Xin Dong, Yan Li, Richard G F Visser, Yuling Bai, Zhenchuan Mao, Shengping Zhang, Bingyan Xie","doi":"10.1111/mpp.70078","DOIUrl":null,"url":null,"abstract":"<p><p>Cucumber production is seriously constrained by Meloidogyne incognita. Because no resistance resources to the pathogen have been reported, disabling susceptibility genes may represent a novel breeding strategy to introduce resistance against this nematode in cucumber. Here, we studied the clade V MLO genes for their involvement in the interaction between cucumber and M. incognita. Our results showed that Arabidopsis clade V MLO mutants were resistant to M. incognita. Cucumber has three clade V MLO genes, CsaMLO1, CsaMLO8 and CsaMLO11, with upregulated expression upon inoculation with M. incognita. Heterologous overexpression of CsaMLO1, CsaMLO8 and CsaMLO11 in Arabidopsis mutants restored susceptibility to varying degrees. Silencing and knockout of individual clade V MLO genes in cucumber reduced susceptibility to M. incognita. The cucumber CRISPR mutants produced similar fruits as the wild type (WT) did. Although the yields of two single mutants (M11<sup>1</sup> and M11<sup>2</sup>) and two double mutants (M8<sup>1</sup> M11<sup>1</sup> and M8<sup>1</sup> M11<sup>2</sup>) were reduced compared to WT, the yields of M8<sup>1</sup> and M8<sup>2</sup> were not decreased. In summary, clade V MLO genes function as susceptibility genes for M. incognita in cucumber. Among them, CsaMLO8 may be the most promising candidate for M. incognita resistance breeding in cucumber.</p>","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 4","pages":"e70078"},"PeriodicalIF":4.8000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950635/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular plant pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1111/mpp.70078","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Cucumber production is seriously constrained by Meloidogyne incognita. Because no resistance resources to the pathogen have been reported, disabling susceptibility genes may represent a novel breeding strategy to introduce resistance against this nematode in cucumber. Here, we studied the clade V MLO genes for their involvement in the interaction between cucumber and M. incognita. Our results showed that Arabidopsis clade V MLO mutants were resistant to M. incognita. Cucumber has three clade V MLO genes, CsaMLO1, CsaMLO8 and CsaMLO11, with upregulated expression upon inoculation with M. incognita. Heterologous overexpression of CsaMLO1, CsaMLO8 and CsaMLO11 in Arabidopsis mutants restored susceptibility to varying degrees. Silencing and knockout of individual clade V MLO genes in cucumber reduced susceptibility to M. incognita. The cucumber CRISPR mutants produced similar fruits as the wild type (WT) did. Although the yields of two single mutants (M111 and M112) and two double mutants (M81 M111 and M81 M112) were reduced compared to WT, the yields of M81 and M82 were not decreased. In summary, clade V MLO genes function as susceptibility genes for M. incognita in cucumber. Among them, CsaMLO8 may be the most promising candidate for M. incognita resistance breeding in cucumber.
期刊介绍:
Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.