Chinese Ophthalmic Research最新文献_第6页

左旋多巴治疗大龄患者屈光参差性弱视疗效的多因素分析左旋多巴治疗大龄患者屈光参差性弱视疗效的多因素分析

Chinese Ophthalmic Research Pub Date : 2010-07-10 DOI: 10.3969/J.ISSN.1003-0808.2010.07.021

张荻, 吴小影, 刘双珍, 王丽娅

引用次数: 0

Targeting therapy of pigment epithelium derived factor mediated by ultrasound activated immunoliposome for choroidal neovascularization 超声激活免疫脂质体介导的色素上皮衍生因子靶向治疗脉络膜新生血管

Chinese Ophthalmic Research Pub Date : 2010-07-10 DOI: 10.3969/J.ISSN.1003-0808.2010.07.004

李涛, 韩勇, 张虹, 徐玲娟, 向艳

{"title":"Targeting therapy of pigment epithelium derived factor mediated by ultrasound activated immunoliposome for choroidal neovascularization","authors":"李涛, 韩勇, 张虹, 徐玲娟, 向艳","doi":"10.3969/J.ISSN.1003-0808.2010.07.004","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.07.004","url":null,"abstract":"目的研究超声联合载色素上皮源性因子(PEDF)免疫纳米脂质体对脉络膜新生血管(CNV)的靶向治疗作用.方法以CNV内皮细胞上血管内皮生长因子受体-2(VEGFR-2)为靶向位点,将其配体寡肽连接到纳米脂质体上,再反向装载PEDF,制备能与CNV内皮细胞特异性结合的免疫纳米脂质体.对48只BN大鼠右眼行半导体倍频激光(波长为532nm)光凝术建立CNV模型.光凝后7d,实验组鼠尾静脉分别注射游离PEDF、载PEDF脂质体、载PEDF免疫脂质体,联合眼局部超声波辐照20s,并设立玻璃体腔注射PEDF组和对照组.光凝后14d,脉络膜巩膜冰冻切片观察免疫脂质体对CNV的靶向性.FITC-右旋糖酐标记脉络膜巩膜铺片行新生血管定量分析.结果载PEDF免疫脂质体可特异性靶向结合CNV.CNV与FITC标记的PEDF免疫纳米脂质体结合而呈现绿色荧光,正常脉络膜血管不显现荧光.玻璃体腔注射PEDF组及鼠尾静脉注射游离PEDF、载PEDF脂质体、载PEDF免疫脂质体组CNV面积均较对照组减少,差异有统计学意义(P＜0.05).超声联合PEDF免疫纳米脂质体组对CNV的抑制作用强于玻璃体腔注射PEDF组(P＜0.05).结论动物实验证实PEDF免疫纳米脂质体可特异性结合CNV细胞,超声可增强PEDF免疫纳米脂质体对CNV的抑制作用,该方法有望为眼新生血管的治疗提供新途径。","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"141 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123632729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of pigment epithelium-derived factor on oxygen-induced retinopathy in rats 色素上皮衍生因子对大鼠氧致视网膜病变的影响

Chinese Ophthalmic Research Pub Date : 2010-07-10 DOI: 10.3969/J.ISSN.1003-0808.2010.07.007

陈晓隆, 谷瑞东, 底煜, 刘鹤南

{"title":"Effects of pigment epithelium-derived factor on oxygen-induced retinopathy in rats","authors":"陈晓隆, 谷瑞东, 底煜, 刘鹤南","doi":"10.3969/J.ISSN.1003-0808.2010.07.007","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.07.007","url":null,"abstract":"目的观察色素上皮衍生因子(PEDF)对高氧诱导的新生鼠视网膜病变模型的影响.方法出生7d Wistar大鼠50只,分为空气组、空气PEDF组、高氧组、高氧PBS组和高氧PEDF组5组.氧箱内饲养5d建立高氧诱导的新生鼠视网膜病变模型,12、14、16d时腹腔内注射PEDF或PBS.在鼠龄17d时,进行视网膜形态学及组织病理学检测,以及血管直径测量、周边视网膜血管覆盖率测定及视网膜新生血管计数.结果视网膜铺片可见高氧组、高氧PBS组较空气组、空气PEDF组新生血管丛增多,高氧PEDF组较高氧组、高氧PBS组视网膜新生血管减少.组织病理学检测突破视网膜内界膜的视网膜新生血管计数可见高氧PEDF组视网膜血管直径明显高于高氧组(P＜0.01)、与空气组比较差异无统计学意义(P＞0.05);高氧组周边视网膜血管覆盖率明显高于高氧PEDF组(P＜0.01)、与空气组相比差异无统计学意义(P＞0.05);高氧组视网膜新生血管数量明显高于高氧PEDF组(P＜0.01)、与空气组相比差异无统计学意义(P＞0.05).结论 PEDF对高氧诱导的新生鼠视网膜病变模型具有抑制作用。","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116901245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of compound rhodiola cretinii on VEGF expression in retina of rats with diabetes mellitus 复方红景天对糖尿病大鼠视网膜VEGF表达的影响

Chinese Ophthalmic Research Pub Date : 2010-06-10 DOI: 10.3969/J.ISSN.1003-0808.2010.06.009

赵红姝, 魏文斌

{"title":"Effect of compound rhodiola cretinii on VEGF expression in retina of rats with diabetes mellitus","authors":"赵红姝, 魏文斌","doi":"10.3969/J.ISSN.1003-0808.2010.06.009","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.06.009","url":null,"abstract":"目的观察中药高山红景天组方对糖尿病大鼠糖尿病视网膜病变(DR)早期血管内皮生长因子(VEGF)表达的影响.方法采用腹腔注射链脲佐菌素(STZ)的方法建立糖尿病大鼠模型.随机分为糖尿病模型治疗组、糖尿病模型未治疗组、正常给药组、正常对照组.造模成功4个月后糖尿病模型治疗组及正常给药组给予高山红景天3g/kg、川芎3g/kg、丹参3g/kg、甘草3g/kg,水煎灌胃,每日1次,正常对照组及糖尿病模型未治疗组均给予凉开水灌胃,每日1次,共1个月.处死大鼠,摘除眼球行VEGF免疫组织化学染色和逆转录-聚合酶链反应(RT-PCR)半定量分析VEGF mRNA的表达.结果糖尿病模型未治疗组与正常对照组间大鼠体重、血糖比较差异均有统计学意义(P＜0.01),糖尿病模型治疗组与正常对照组间大鼠体重、血糖差异均有统计学意义(P＜0.01).VEGF免疫组织化学染色结果显示,糖尿病模型未治疗组、糖尿病模型治疗组、正常对照组及正常给药组视网膜组织VEGF的蛋白表达积分光密度(IOD)值分别为0.316±0.046、0.149±0.038、0.126±0.028、0.130±0.038;各组面密度(AD)值分别为0.463±0.185、0.121±0.015、0.122±0.007、0.121±0.011.糖尿病模型治疗组与糖尿病模型未治疗组相比、正常对照组与糖尿病模型未治疗组相比IOD值及AL值差异均有统计学意义(P＜0.01).正常给药组及正常对照组组间比较差异无统计学意义(P＞0.05).RT-PCR检测VEGF mRNA表达显示,糖尿病模型治疗组对目的基因有表达,表达水平较低,糖尿病模型未治疗组对目的基因有表达,表达水平较高;糖尿病模型治疗组与未治疗组比较差异有统计学意义(P＜0.01);正常对照组对目的基因几乎无表达.结论高山红景天组方口服可使糖尿病鼠视网膜VEGF的表达减少,为DR的治疗提供新方法。","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116239853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic analysis of human lenses nucleus in cataractous eye 白内障眼晶状体核的蛋白质组学分析

Chinese Ophthalmic Research Pub Date : 2010-06-10 DOI: 10.3969/J.ISSN.1003-0808.2010.06.005

王丹丹, 朱忠欣, 蔡军勇, 金利泰, 丛维涛, 赵云娥

{"title":"Proteomic analysis of human lenses nucleus in cataractous eye","authors":"王丹丹, 朱忠欣, 蔡军勇, 金利泰, 丛维涛, 赵云娥","doi":"10.3969/J.ISSN.1003-0808.2010.06.005","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.06.005","url":null,"abstract":"目的研究年龄相关性白内障晶状体核不同组分晶状体蛋白的组成.方法取12例年龄相关性白内障晶状体软核,提取晶状体核中的水溶性蛋白及尿溶性蛋白进行二维电泳及UPD-蛋白质染料染色试剂盒对凝胶染色后,用Image Master 2D Platinum 6.0软件对获得的蛋白图谱进行分析,再利用MALDI-TOF MS分析及数据库搜索对差异表达的蛋白质点进行鉴定.结果二维电泳结果显示,年龄相关性白内障晶状体核中水溶性蛋白和尿溶性蛋白大部分为相对分子质量14 400～97 400,pI 5～9.高丰度蛋白质斑点相对分子质量分布在20 000～31 000,pI 6～8区域内.年龄相关性白内障晶状体核水溶性蛋白二维电泳图识别出31个蛋白质斑点,尿溶性蛋白二维电泳图识别出26个蛋白质斑点,相差2倍以上表达的差异点有14个.对选取的蛋白质斑点进行MALDI-TOF MS分析,经数据库检索后鉴定出6种蛋白质,其中3种蛋白质表达下调(γS-晶状体蛋白、βA3-晶状体蛋白、βA4-晶状体蛋白),1种蛋白质表达上调(βB1-晶状体蛋白),2种蛋白质表达缺失(截取的人βB1-晶状体蛋白A链、截取的人γD-晶状体蛋白X链).结论年龄相关性白内障晶状体核不同组分晶状体蛋白中主要由β-晶状体蛋白组成,与尿溶性晶状体蛋白相比,水溶性蛋白鉴定出γD-晶状体蛋白,而βA4-晶状体蛋白阙如。","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134217333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of resistin in serum of TAO patients 抵抗素在TAO患者血清中的表达

Chinese Ophthalmic Research Pub Date : 2010-06-10 DOI: 10.3969/J.ISSN.1003-0808.2010.06.018

L. Hongxia, W. Zhongyao

{"title":"Expression of resistin in serum of TAO patients","authors":"L. Hongxia, W. Zhongyao","doi":"10.3969/J.ISSN.1003-0808.2010.06.018","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.06.018","url":null,"abstract":"BackgroundResistin is the member of human cytokines,but its function is below understood.Research revealed that the expression of resistin in serum is regulated by sexual gland,thyroid hormone.ObjectiveThe goal of this study was to investigate the expression of resistin in the serum of TAO patients.MethodsThe serum samples were obtained from 35 patients with thyroid-associated ophthalmopathy(TAO).These patients rejected the administration of glucocorticoid and immunity inhibitor within 3 months and were assigned to inactive TAO group(CAS4,18 cases)and active TAO group(CAS≥4,17 cases).Fifteen normal subjects were as control group.The level of resistin in serum was detected using ELISA kit.Written informed consent was obtained from each individual at initial of the medical process.Results No significant difference was found in serum resistin level between inactive TAO group and active TAO group(12.103±5.042 versus 13.470±3.749)mg/L(t=0.906,P=0.371).However,serum resistin level was significant declined in inactive TAO group or active TAO group in comparison with normal group(12.103±5.042 versus 21.894±10.589;13.470±3.749 versus 21.894±10.589)mg/L(t=3.074,P=0.004;t=3.485,P=0.001).No obvious difference was seen between male patients and female patients(13.175±4.483 versus 12.335±4.511)mg/L(t=0.552,P=0.585).Serum resistin level was gradually elevated with the increase of age in TAO patients(F=24.978,P=0.000).ConclusionThe serum resistin level of TAO patients is lower than the control subjects,suggesting that the level of serum resistin is regulated by thyroid hormones.The serum resistin level increases with age in TAO patient.","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125028195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current research incomplications after intravitreal injection of bevacizumab 贝伐单抗玻璃体内注射后并发症的研究现状

Chinese Ophthalmic Research Pub Date : 2010-06-10 DOI: 10.3969/J.ISSN.1003-0808.2010.06.025

刘晓娟

引用次数: 0

Exclusive mapping for all known locus linked with congenital iris and retinochoroidal coloboma in a Chinese family 对一个中国家庭先天性虹膜和视网膜脉络膜结肠瘤的所有已知位点进行独家定位

Chinese Ophthalmic Research Pub Date : 2010-06-10 DOI: 10.3969/CMA.J.ISSN.1003-0808.2010.06.017

布娟, 董佳梅, 杜伟, 李静, 卓彦玲, 王乐今

引用次数: 0

Toric人工晶状体矫正散光的临床效果评价 Toric人工晶状体矫正散光的临床效果评价

Chinese Ophthalmic Research Pub Date : 2010-06-10 DOI: 10.3969/J.ISSN.1003-0808.2010.06.026

刘冬梅, 毕宏生, 蔡婉婷, 吴建峰, 解孝锋, 刘翠红

引用次数: 0

Avastin前房内注射及滴眼液治疗眼前段新生血管一例 Avastin前房内注射及滴眼液治疗眼前段新生血管一例

Chinese Ophthalmic Research Pub Date : 2010-05-10 DOI: 10.3969/J.ISSN.1003-0808.2010.05.015

宋莉, 沙翔垠, 罗春云

引用次数: 0