Chinese Ophthalmic Research最新文献

Effects of melatonin on expression of manganese superoxide dismutase and cuproznic superoxide dismutase in retina in early diabetic rats 褪黑素对早期糖尿病大鼠视网膜锰超氧化物歧化酶和铜丙二酸超氧化物歧化酶表达的影响

Chinese Ophthalmic Research Pub Date : 2010-12-10 DOI: 10.3969/J.ISSN.1003-0808.2010.12.002

李晓艳, 张卯年

{"title":"Effects of melatonin on expression of manganese superoxide dismutase and cuproznic superoxide dismutase in retina in early diabetic rats","authors":"李晓艳, 张卯年","doi":"10.3969/J.ISSN.1003-0808.2010.12.002","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.12.002","url":null,"abstract":"目的观察褪黑素对早期糖尿病大鼠视网膜锰超氧化物歧化酶(MnSOD)、铜锌超氧化物歧化酶(Cu/ZnSOD)活性及基因表达变化的影响,以揭示褪黑素对早期糖尿病大鼠视网膜的抗氧化作用机制.方法 54只SD大鼠按随机数字表法分为正常对照组、糖尿病组及褪黑素组,每组18只.糖尿病组及褪黑素组大鼠一次性腹腔注射链脲佐菌素(STZ)60mg/kg,升高血糖至＞16.7mmol/L,建立糖尿病大鼠模型,褪黑素组大鼠于STZ注射后第2天给予褪黑素灌胃,每日10mg/kg.于造模后4、8、12周3个时间点处死各组6只大鼠制备视网膜匀浆.应用黄嘌呤氧化酶法检测视网膜组织中总SOD、Cu/ZnSOD及MnSOD的活性;应用实时荧光定量PCR法检测视网膜组织中MnSOD mRNA及Cu/ZnSOD mRNA的表达量.结果模型建立后4、8、12周时糖尿病组和褪黑素组大鼠血糖水平明显高于正常对照组大鼠,差异均有统计学意义(P＜0.01).造模后4、8、12周时各时间点糖尿病组大鼠视网膜中总 SOD、MnSOD活性明显低于正常对照组,8周及12周时褪黑素组大鼠视网膜中总SOD、MnSOD活性明显高于糖尿病组,差异均有统计学意义(P＜0.01).糖尿病组大鼠视网膜中Cu/ZnSOD活性在8周及12周时明显降低,与正常对照组比较差异均有统计学意义(P＜0.01),12周时褪黑素组较糖尿病组有所升高,差异有统计学意义(P＜0.01).各时间点糖尿病组大鼠MnSOD mRNA在视网膜中的表达量与正常对照组相比明显下降,褪黑素组MnSOD mRNA的表达明显高于糖尿病组,差异均有统计学意义(P＜0.01);8周及12周时糖尿病组Cu/ZnSOD mRNA的表达量较正常对照组明显下降,12周时褪黑素组Cu/ZnSOD mRNA的表达明显高于糖尿病组,差异有统计学意义 (P＜0.01).结论糖尿病早期,褪黑素主要通过提高视网膜MnSOD的活性及增强其基因表达减轻视网膜的氧化损伤。","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125952700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of minocycline on leakage of retinal microvessels induced by glycation end products and its mechanism 米诺环素对糖基化终产物致视网膜微血管渗漏的影响及其机制

Chinese Ophthalmic Research Pub Date : 2010-12-10 DOI: 10.3969/J.ISSN.1003-0808.2010.12.001

陈永东, 许迅, 吴强, 郑志, 朱冬青, 顾青

{"title":"Effects of minocycline on leakage of retinal microvessels induced by glycation end products and its mechanism","authors":"陈永东, 许迅, 吴强, 郑志, 朱冬青, 顾青","doi":"10.3969/J.ISSN.1003-0808.2010.12.001","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.12.001","url":null,"abstract":"目的研究基质金属蛋白酶-9(MMP-9)在糖基化终末产物(AGEs)所致血-视网膜屏障损害中的作用,评估米诺环素对AGEs所致血-视网膜屏障损害的治疗作用.方法 36只SD大鼠用随机数字表法分为牛血清白蛋白(BSA)组、AGEs-BSA组和AGEs-BSA+米诺环素组.BSA组和AGEs-BSA组分别自尾静脉注入BSA和AGEs-BSA,每日1次,每次40mg/kg,AGEs-BSA+米诺环素组在每日注射AGEs-BSA的同时隔日腹腔注射米诺环素45mg/kg.14d后,每组取6只大鼠用伊文思蓝(EB)法观察并比较各组视网膜血管的通透性,其余大鼠处死后,采用RT-PCR及Western blot法观察视网膜MMP-9的表达.结果 AGEs-BSA组MMP-9 mRNA和蛋白水平均较BSA组升高,差异有统计学意义(P＜0.01),同时,视网膜微血管渗透性也显著升高(P＜0.01).而在AGEs-BSA+米诺环素组大鼠视网膜基因和蛋白水平的MMP-9均被抑制(P＜0.01),同时视网膜微血管渗漏被部分逆转(P＜0.01).视网膜微血管的渗透性与MMP-9蛋白的表达呈正相关(r=0.891,P=0.000).结论 AGEs所致的视网膜微血管渗漏与MMP-9的高表达有密切关系,AGEs的作用至少部分是通过MMP-9实现的.米诺环素能部分逆转AGEs-MMP-9途径所致的血管渗漏。","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121425035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical evaluation of application of quantitative micro-keratoscope 定量显微角膜镜应用的临床评价

Chinese Ophthalmic Research Pub Date : 2010-12-10 DOI: 10.3969/J.ISSN.1003-0808.2010.12.011

张加裕, 施明光

{"title":"Clinical evaluation of application of quantitative micro-keratoscope","authors":"张加裕, 施明光","doi":"10.3969/J.ISSN.1003-0808.2010.12.011","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.12.011","url":null,"abstract":"目的通过多家眼科单位对显微定量角膜检查镜(简称角膜镜)的临床试用检验角膜镜检查角膜散光度的准确性和稳定性.方法前瞻性多中心临床试验研究.4家眼科单位分别用角膜镜和角膜曲率计对被检者156例156眼(均取右眼)进行角膜散光测量,其中角膜镜每眼测3次,角膜曲率计每眼测1次,将角膜镜测量均值与角膜曲率计测量值进行比较,采用组内相关系数分析角膜镜3次测量间的稳定性,采用Bland-Altman散点图评价角膜镜与角膜曲率计测量散光度的准确性和一致性.结果角膜镜与角膜曲率计测量角膜散光度均值分别为(-1.96±1.16)D和(-1.84±1.17)D,二者呈高度线性相关(r=0.929,P=0.000),配对t检验示二者差异有统计学意义(t=-3.020,P=0.003).角膜镜与角膜曲率计测量角膜散光度差值为(-0.12±0.44)D.角膜镜与角膜曲率计测量角膜散光轴向的中位数均为0°,配对Wilcoxon符号秩检验示差异无统计学意义(Z=-1.775,P=0.076).单因素方差分析显示角膜镜散光度3次测量间差异无统计学意义(F=0.153,P=0.859).角膜镜对角膜散光度测量的组内相关系数(ICC)为0.975,对角膜散光轴向测量的ICC为0.988.角膜镜和角膜曲率计测量角膜散光度的一致性范围(差值均数±1.96SD)为(-1.00,0.76)D;将测量范围0～-8D分为0～、-1D～、-2D～、-4～-8D组段,结果显示角膜镜和角膜曲率计对各组段散光度测量的一致性范围均小于或接近于1.0D.结论角膜镜测量角膜散光度具有良好的准确性和稳定性。","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133851742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

百草枯中毒致眼部、口腔及皮肤病损一例百草枯中毒致眼部、口腔及皮肤病损一例

Chinese Ophthalmic Research Pub Date : 2010-12-10 DOI: 10.3969/J.ISSN.1003-0808.2010.12.020

仝春梅, 王超英, 刘迎庆, 韩宝雁

引用次数: 0

Effects of curcumin on expression of macrophage migration inhibitory factor in retina of diabetic rats 姜黄素对糖尿病大鼠视网膜巨噬细胞迁移抑制因子表达的影响

Chinese Ophthalmic Research Pub Date : 2010-12-10 DOI: 10.3969/J.ISSN.1003-0808.2010.12.008

刘娟, 游志鹏

{"title":"Effects of curcumin on expression of macrophage migration inhibitory factor in retina of diabetic rats","authors":"刘娟, 游志鹏","doi":"10.3969/J.ISSN.1003-0808.2010.12.008","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.12.008","url":null,"abstract":"目的检测巨噬细胞游走抑制因子(MIF)在糖尿病大鼠视网膜中的表达,探讨MIF在糖尿病视网膜病变(DR)中的作用及姜黄素早期干预对其表达的影响.方法对30只清洁级SD大鼠行腹腔注射质量分数1%链脲佐菌素(STZ)诱导建立糖尿病动物模型,选取其中的20只随机分为糖尿病组和姜黄素治疗组,各10只,另取10只正常匹配的大鼠作为正常对照组.姜黄素治疗组自STZ注射后3d起给予含羧甲基纤维素的姜黄素200mg/(kg*d)灌胃,共8周,正常对照组和糖尿病组给予等量的羧甲基纤维素灌胃.动物处死后收集各组大鼠眼球,苏木精-伊红染色观察大鼠视网膜组织的病理变化;采用ELISA法检测各组大鼠视网膜组织中MIF的表达;应用免疫组织化学法观察各实验组大鼠视网膜中MIF的表达及定位;透射电镜下观察大鼠视网膜超微结构的改变.结果与正常对照组比较,糖尿病组大鼠和姜黄素治疗组大鼠血糖明显升高,差异均有统计学意义(P＜0.01);姜黄素治疗组大鼠血糖水平明显低于糖尿病组,差异有统计学意义(P＜0.01).与正常对照组比较,糖尿病组和姜黄素治疗组大鼠体重明显下降,差异均有统计学意义(P＜0.01);姜黄素治疗组大鼠体重明显大于糖尿病组,差异有统计学意义(P＜0.01).糖尿病组大鼠视网膜变薄,但姜黄素治疗组大鼠视网膜接近正常.免疫组织化学检测表明,糖尿病组视网膜组织中含有大量MIF阳性细胞,姜黄素治疗组视网膜中MIF表达明显少于糖尿病组(P＜0.01).ELISA法检测结果显示,糖尿病组MIF的表达较正常对照组显著升高(P＜0.01);姜黄素治疗组MIF的表达较糖尿病组明显下降(P＜0.01).透射电镜下糖尿病组大鼠视网膜光感受器内外节水肿,毛细血管内皮细胞和周细胞染色体边集;姜黄素治疗组大鼠视网膜超微结构的改变轻微.结论 DR与视网膜的炎性改变有关,视网膜组织中MIF的高表达参与早期DR的发生发展.姜黄素可下调MIF的表达,其早期干预可延缓DR的发病进程。","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131294397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A preliminary study on genotype of candida tropicalis isolated from eyes infection 眼部感染分离热带念珠菌基因型的初步研究

Chinese Ophthalmic Research Pub Date : 2010-12-10 DOI: 10.3969/J.ISSN.1003-0808.2010.12.003

宋绪华, 邢健强, 陈海, 陈梨萍

{"title":"A preliminary study on genotype of candida tropicalis isolated from eyes infection","authors":"宋绪华, 邢健强, 陈海, 陈梨萍","doi":"10.3969/J.ISSN.1003-0808.2010.12.003","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.12.003","url":null,"abstract":"目的建立随机扩增DNA多态性(RAPD)反应体系对热带念珠菌进行基因分型.方法从14例真菌感染的眼部病变处取材并根据真菌菌落外观、菌丝、孢子及菌细胞形态进行菌种鉴定,采用Neo-Sensitab抗真菌药敏纸片进行药物敏感试验.分别提取14株眼部感染热带念珠菌临床分离株的基因组DNA,依次用随机引物OPA-18、OPE-18、RSD-6、OPG-10对其进行PCR扩增,图谱分析后用非加权配对算术平均法(UPGMA)绘出亲缘关系聚类图.结果 14株热带念珠菌对两性霉素B、酮康唑、氟康唑和氟胞嘧啶的敏感率依次为50.00%、85.71%、100.00%和100.00%.采用引物OPG-10和RSD-6构建的RAPD-PCR体系可以扩增出质量较好的带谱,多态性位点依次为71.42%和72.41%.采用引物OPG-10进行RAPD-PCR产物构建的亲缘关系聚类图中,对两性霉素B敏感的第1、2、3、4、5、6株热带念珠菌与对两性霉素B耐药的第14株热带念珠菌聚成一簇,而其余对两性霉素B耐药的第7、8、9、10、11、12、13株聚成一簇.采用引物RSD-6进行RAPD-PCR产物构建的亲缘关系聚类图中,对两性霉素B敏感的第1、2、3、4、5、6株聚成一簇,而对两性霉素B耐药的第7、8、9、10、11、12、13、14株聚成一簇.结论采用引物RSD-6构建的RAPD-PCR反应体系可以较好地进行热带念珠菌的分子分型,对两性霉素B敏感株和耐药株进行鉴定。","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132260259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of modulation transfer function between aspheric and spherical intraocular lens with different entire spherical aberrations 不同全球像差的非球面与球形人工晶状体调制传递函数的比较

Chinese Ophthalmic Research Pub Date : 2010-12-10 DOI: 10.3969/J.ISSN.1003-0808.2010.12.015

邢晓杰, 宋慧, 汤欣

{"title":"Comparison of modulation transfer function between aspheric and spherical intraocular lens with different entire spherical aberrations","authors":"邢晓杰, 宋慧, 汤欣","doi":"10.3969/J.ISSN.1003-0808.2010.12.015","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.12.015","url":null,"abstract":"目的比较不同非球面和球面人工晶状体(IOL)在不同全眼球差和空间频率下的高阶像差调制传递函数(MTF)值,为非球面IOL的球差设计及人眼的最佳球差值提供理论基础.方法选取白内障术后3个月相同材料、不同设计的非球面IOL共102眼(其中Z9003 IOL 40眼,PY60AD IOL 20眼,920H IOL 15眼,Akreos AO IOL 15眼,IQ IOL 12眼)和球面IOL共60眼(其中AR40e IOL 40眼,YA60BB IOL 20眼)进行iTrace视觉功能分析仪检查,记录6mm瞳孔直径下的全眼高阶像差成分及MTF值,将非球面IOL组根据全眼球差的大小和方向分组,连同球面IOL组比较不同频率下各组高阶像差的MTF值.结果在不同全眼球差下,非球面IOL各组总高阶像差值比较,差异有统计学意义(P＜0.05),而彗差和三叶草像差间差异无统计学意义(P＞0.05).除15cpd外,其他空间频率下球差0.05～0.15μm组非球面IOL的MTF值最高,但各组间差异无统计学意义(P＞0.05).在低空间频率下,0.05μm≤球差＜0.15μm组和0.15μm≤球差＜0.5μm组比较MTF值差异有统计学意义(P＜0.05).非球面IOL和球面IOL的全眼高阶像差各成分及不同空间频率MTF值的比较,差异均有统计学意义(P＜0.05).结论非球面IOL组较小的眼内球差较球面IOL组对MTF有更大的提高作用.对于非球面IOL植入术后6mm瞳孔直径下全眼球差在0.05～0.15μm,即+0.1μm左右可能具有更好的客观视觉质量。","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133533908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of human keratocyte proliferation following the transfection of cyclinD1 antisense gene 转染cyclinD1反义基因对人角质细胞增殖的抑制作用

Chinese Ophthalmic Research Pub Date : 2010-11-10 DOI: 10.3969/J.ISSN.1003-0808.2010.11.005

荣蓓, 晏晓明

{"title":"Inhibition of human keratocyte proliferation following the transfection of cyclinD1 antisense gene","authors":"荣蓓, 晏晓明","doi":"10.3969/J.ISSN.1003-0808.2010.11.005","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.11.005","url":null,"abstract":"目的探讨反义cyclinD1基因转染后人角膜基质细胞增生的改变.方法人角膜组织来源于北京大学第一医院眼库体外培养人角膜基质细胞,采用第2代或第3代细胞用于实验.利用阳离子脂质体lipofectamine介导反义cyclinD1基因转染入体外培养的人角膜基质细胞.同时设非转染组(以PBS代替cyclinD1质粒及lipofectamine)及lipofectamine转染组(以PBS代替反义cyclinD1质粒)作为对照.应用免疫细胞化学法观察lipofectamine介导反义cyclinD1基因转染对人角膜基质细胞中cyclinD1蛋白表达的影响,应用四甲基偶氮唑盐(MTT)法观察其对人角膜基质细胞增生的影响,并用质量分数0.5%锥虫蓝染色观察活细胞比率.结果非转染组和lipofectamine转染组均有大量人角膜基质细胞的细胞核cyclinD1染色呈棕褐色,反义cyclinD1转染组只有少量人角膜基质细胞细胞核cyclinD1染色呈棕褐色.与非转染组和lipofectamine转染组比较,反义cyclinD1转染组在转染后2、6、10d cyclinD1蛋白在培养的人角膜基质细胞中的表达明显降低,差异均有统计学意义(t=6.050、t=5.180、t=5.040,P＜0.01;t=6.190、t=5.670、t=5.010,P＜0.01),而非转染组与lipofectamine转染组间比较,差异均无统计学意义(t=1.120、t=1.310、t=1.010,P＞0.05).转染后2、6、10d,反义cyclinD1转染组人角膜基质细胞的A570值分别低于非转染组和lipofectamine转染组,差异均有统计学意义(t=5.830、t=5.210、t=4.920,P＜0.01;t=5.130、t=5.010、t=4.850,P＜0.01),说明抑制了基质细胞的增生,而非转染组与lipofectamine转染组间比较,差异均无统计学意义(t=1.110、t=1.230、t=1.120,P＞0.05).0.5%锥虫蓝染色显示各组活细胞比率均在90%以上,未见明显的细胞毒性.结论阳离子脂质体介导的反义cyclinD1基因转染在转染后一定时间内可降低人角膜基质细胞中cyclinD1蛋白的表达,抑制人角膜基质细胞的增生,未见明显细胞毒性,为角膜基质过度愈合反应的基因治疗奠定了基础。","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128339026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship of advanced glycation end products and mydriasis 晚期糖基化终产物与丝虫病的关系

Chinese Ophthalmic Research Pub Date : 2010-11-10 DOI: 10.3969/J.ISSN.1003-0808.2010.11.013

黄春梅, 李志红, 赵东, 袁中芳, 李建平

{"title":"Relationship of advanced glycation end products and mydriasis","authors":"黄春梅, 李志红, 赵东, 袁中芳, 李建平","doi":"10.3969/J.ISSN.1003-0808.2010.11.013","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.11.013","url":null,"abstract":"目的探讨虹膜中糖基化终末产物(AGEs)的表达及血浆AGEs质量浓度与糖尿病患者瞳孔功能障碍的关系,并探讨其发病机制.方法对糖尿病并发白内障患者的瞳孔散大难易度与单纯白内障患者进行对照研究.依据白内障手术前瞳孔散大是否能够达到6mm,将2型糖尿病并发白内障患者分为瞳孔功能异常组24例和瞳孔功能正常组21例,单纯白内障组患者21例作为对照.采用竞争性酶联免疫吸附分析法测定各组患者血浆AGEs的吸光度(A)值;采用免疫组织化学法分别检测上述各组患者虹膜组织中AGEs的表达强度.结果 3组在性别、年龄、眼压和糖尿病病程方面差异均无统计学意义(P＞0.05).瞳孔功能异常组、瞳孔功能正常组和单纯白内障组血浆AGEs质量浓度分别为7.625±1.69、5.904±1.27和3.726±1.35,组间差异均有统计学意义(F=312.431,P＜0.05);瞳孔功能正常组和单纯白内障组的血浆AGEs质量浓度明显低于瞳孔功能异常组,差异均有统计学意义(t1=2.017,P＜0.05;t2=2.018,P＜0.05).瞳孔功能异常组、瞳孔功能正常组和单纯白内障组AGEs在虹膜组织中的表达强度值分别为109.13±19.47、79.71±13.06和40.92±11.91,差异有统计学意义(F=188.32,P＜0.05),瞳孔功能正常组和单纯白内障组的AGEs表达值明显低于瞳孔功能异常组,差异均有统计学意义(t1=2.017,P＜0.05;t2=2.023,P＜0.05).结论 AGEs在糖尿病瞳孔功能异常患者虹膜组织中呈高表达,其血浆AGEs质量浓度亦明显升高,提示AGEs在虹膜组织中的积聚与糖尿病患者瞳孔功能异常有关,可能是其发病机制之一。","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"64 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124844342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vision electrophysiology assessment for color vision deficiency 色觉缺陷的视觉电生理评估

Chinese Ophthalmic Research Pub Date : 2010-11-10 DOI: 10.3969/J.ISSN.1003-0808.2010.11.021

安晶

引用次数: 0