Chinese Ophthalmic Research最新文献_第7页

Early diagnosis of primary open-angle glaucoma in patients with high myopia after laser in situ keratomileusis 高度近视激光原位角膜磨砂术后原发性开角型青光眼的早期诊断

Chinese Ophthalmic Research Pub Date : 2010-05-10 DOI: 10.3969/J.ISSN.1003-0808.2010.05.018

曾锦, 郭海科, 崔颖, 谢文娟, 李仲明, 廖伟雄

{"title":"Early diagnosis of primary open-angle glaucoma in patients with high myopia after laser in situ keratomileusis","authors":"曾锦, 郭海科, 崔颖, 谢文娟, 李仲明, 廖伟雄","doi":"10.3969/J.ISSN.1003-0808.2010.05.018","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.05.018","url":null,"abstract":"目的探讨高度近视行准分子激光角膜原位磨镶术(LASIK)后开角型青光眼患者的临床特征.方法回顾性分析2008年7月-2009年6月以屈光回退或眩光为主诉复诊的高度近视行LASIK术后患者13例25眼的临床资料;所有患者屈光回退现象均发生在术后2a,其中男8例,女5例;平均年龄30.1岁,均进行视力、屈光度、角膜地形图、Goldman眼压、眼轴、视野及OCT检查,原发性开角型青光眼的诊断依据全国青光眼学组建议的诊断标准,所有患者术前均签署知情同意书.结果 12例23眼中,术后平均回退屈光度为(-1.57±0.36)D,裸眼视力0.42±0.14;1例患者双眼眩光加重,瞳孔散大,直径为4.5～5.0mm,夜间瞳孔直径为5.8～6.1mm;6例12眼术后校正眼压为21～26mmHg,合并有视网膜神经纤维层(RNFL)变薄及相应的青光眼典型视野改变;7例13眼屈光回退者中OCT检查RNFL变薄者3例5眼,用眼过度致视疲劳者4例8眼.结论高度近视患者行LASIK 2a后出现视力下降及眩光现象,且单纯以屈光回退及光学区过小无法解释者,要考虑到并发开角型青光眼的可能。","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"112 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117241324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

双侧球后视神经炎致视交叉受累一例双侧球后视神经炎致视交叉受累一例

Chinese Ophthalmic Research Pub Date : 2010-05-10 DOI: 10.3969/J.ISSN.1003-0808.2010.05.013

张曙光, 马林昆, 李燕, 袁援生

引用次数: 0

Neuroprotective effect ofpigment epithelium-derived factor on retinal ganglion cells of rats with chronicintraocular hypertension 色素上皮衍生因子对慢性眼压过高大鼠视网膜神经节细胞的神经保护作用

Chinese Ophthalmic Research Pub Date : 2010-05-10 DOI: 10.3969/J.ISSN.1003-0808.2010.05.014

吕炳健, 高晓唯, 王瑞夫, 严宏, 王百忍

{"title":"Neuroprotective effect ofpigment epithelium-derived factor on retinal ganglion cells of rats with chronicintraocular hypertension","authors":"吕炳健, 高晓唯, 王瑞夫, 严宏, 王百忍","doi":"10.3969/J.ISSN.1003-0808.2010.05.014","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.05.014","url":null,"abstract":"目的探讨色素上皮衍生因子(PEDF)对慢性高眼压条件下大鼠视网膜神经节细胞(RGCs)的作用.方法雌性SD大鼠36只,随机分为高眼压+PEDF组(A组)、高眼压+去离子水组(B组)和假手术+PEDF组(C组),A组、B组模型的制作应用巩膜浅层静脉烧烙法建立慢性高眼压模型,C组仅剪开球结膜,不烧烙巩膜浅静脉.A组、C组在模型建立后即刻用10μL微量注射器于大鼠角膜缘后2mm处刺入玻璃体腔,抽出玻璃体2μL,再向玻璃体腔内注射0.05g/L重组大鼠PEDF 2μL,B组同法注入等量的去离子水.在3d和14d后处死动物,摘除眼球,将视网膜组织行冰冻切片,用TUNEL法及Fluoro-Jade(FJ)荧光染色检测各组RGCs的凋亡和变性.结果术后3d、14d时A组和B组眼压均明显升高,与同时间点C组相比差异均有统计学意义(P＜0.05);各组术后3d和14d间的眼压比较差异均无统计学意义(P＞0.05).TUNEL检测显示,A组、C组3d和14dRGCs的凋亡数目均明显少于同时间点B组(P＜0.05),A组14d的凋亡细胞数明显少于3d(P＜0.05).FJ荧光染色结果显示,A组、C组3d和14d变性RGCs数均明显少于B组(P＜0.05),A组14d的变性细胞数较3d时明显减少(P＜0.05).结论玻璃体腔注射PEDF可减少慢性高眼压大鼠RGCs的凋亡和变性。","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"108 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129955889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

白内障术后角膜曲率改变对人工晶状体计算公式准确性的影响白内障术后角膜曲率改变对人工晶状体计算公式准确性的影响

Chinese Ophthalmic Research Pub Date : 2010-05-10 DOI: 10.3969/J.ISSN.1003-0808.2010.05.026

陈剑铭, 王登廷, 魏建兰, 汝亚琴

引用次数: 0

Screening analysis for the causative genes in a Bai family with retinitis pigmentosa 白家系色素性视网膜炎致病基因筛选分析

Chinese Ophthalmic Research Pub Date : 2010-05-10 DOI: 10.3969/J.ISSN.1003-0808.2010.05.021

郭海科, 覃泳杰, 夏泽梅, 孟倩丽, 张洪洋, 金海鹰, 陈艳蕾

引用次数: 0

Susceptibility of CYP1B1 knock-out mouse to glaucoma in a high glucocorticoid environment 高糖皮质激素环境下CYP1B1基因敲除小鼠对青光眼的易感性

Chinese Ophthalmic Research Pub Date : 2010-05-10 DOI: 10.3969/J.ISSN.1003-0808.2010.05.001

吴畅, 姜发纲, 刘晓雯

{"title":"Susceptibility of CYP1B1 knock-out mouse to glaucoma in a high glucocorticoid environment","authors":"吴畅, 姜发纲, 刘晓雯","doi":"10.3969/J.ISSN.1003-0808.2010.05.001","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.05.001","url":null,"abstract":"目的观察高浓度糖皮质激素对CYP1B1-/-小鼠青光眼易感性的影响.方法采用成年CYP1B1-/-小鼠作为实验模型,以同龄C57BL/6J小鼠作为对照.每3天结膜下注射0.04mL倍他米松,用Tonopen眼压(IOP)笔每周定期测定小鼠IOP,于第一次给药前,给药后4、8、12周分别摘除眼球,制备石蜡切片,光学显微镜下观察小鼠视网膜形态和厚度,采用TUNEL法检测视网膜神经节细胞(RGCs)的凋亡.结果与给药前相比,2组小鼠给药后4、8、12周的IOP均较前升高,差异有统计学意义(P＜0.05);CYP1B1-/-小鼠的IOP在给药后8周、12周较C57BL/6J小鼠显著升高,差异有统计学意义(P＜0.05).随着倍他米松注射时间的推移,2组小鼠视网膜纤维层均变薄,各时间组的总体差异有统计学意义(P＜0.05);且CYP1B1-/-小鼠视网膜纤维层厚度在给药后8周、12周较C57BL/6J小鼠显著变薄,差异有统计学意义(P＜0.05).2组小鼠RGCs凋亡的速度与给药前相比均显著增加,差异有统计学意义(P＜0.05);且CYP1B1-/-小鼠与C57BL/6J小鼠相比结果更为显著(P＜0.05).结论在高浓度糖皮质激素的诱导下,CYP1B1-/-小鼠对青光眼的易感性增加。","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127440668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmaceutical treating progression of proliferative vitreoretinopathy 药物治疗增生性玻璃体视网膜病变的进展

Chinese Ophthalmic Research Pub Date : 2010-04-10 DOI: 10.3969/J.ISSN.1003-0808.2010.04.025

焦明菲, 颜华

引用次数: 1

屈光性透明晶状体置换术治疗超高度近视屈光性透明晶状体置换术治疗超高度近视

Chinese Ophthalmic Research Pub Date : 2010-04-10 DOI: 10.3969/J.ISSN.1003-0808.2010.04.024

张春梅, 贾乃伟, 高平, 宋长径

引用次数: 0

Effect of sinvastatin on VE-cadherin in retinas of rat with diabetes 辛伐他汀对糖尿病大鼠视网膜ve -钙粘蛋白的影响

Chinese Ophthalmic Research Pub Date : 2010-04-10 DOI: 10.3969/J.ISSN.1003-0808.2010.04.009

萧鸿, 王志云, 庞东渤, 刘学政

{"title":"Effect of sinvastatin on VE-cadherin in retinas of rat with diabetes","authors":"萧鸿, 王志云, 庞东渤, 刘学政","doi":"10.3969/J.ISSN.1003-0808.2010.04.009","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.04.009","url":null,"abstract":"目的观察链脲佐菌素(STZ)诱导的糖尿病视网膜血管内皮钙黏蛋白(VE-cadherin)的表达及辛伐他汀对VE-cadherin表达的影响,探讨他汀类药物可能的非调脂性治疗作用.方法尾静脉注射STZ法建立糖尿病大鼠模型,造模成功48只.成模次日起,24只大鼠每日给予辛伐他汀20mg/kg灌胃为辛伐他汀组,24只等量生理盐水灌胃为糖尿病组;另选24只大鼠作为正常对照组.分别在造模后2、5、8周各组取8只大鼠采用伊凡思蓝(EB)法检测视网膜的渗透性,免疫组织化学和Western blot法检测各组大鼠视网膜VE-cadherin的表达情况.结果与正常对照组比较,辛伐他汀组和糖尿病组大鼠体重明显下降,差异均有统计学意义(P＜0.01),血糖显著上升,差异均有统计学意义(P＜0.01).与糖尿病组大鼠比较,各时间点辛伐他汀组大鼠体重均明显上升,而血糖均显著下降(P＜0.05).免疫组织化学分析证实,VE-cadherin在糖尿病组大鼠视网膜血管呈黄褐色阳性表达.Western blot分析表明随着糖尿病视网膜病变(DR)的发生发展,糖尿病组视网膜血管表达VE-cadherin的量显著减少,与正常对照组和辛伐他汀组比较,差异均有统计学意义(P＜0.05).糖尿病组和辛伐他汀组EB渗透量明显高于正常对照组(P＜0.01),辛伐他汀组低于糖尿病组(P＜0.05).结论 STZ诱导的糖尿病大鼠视网膜血管中VE-cadherin表达量减少,辛伐他汀可改善这种改变,提示辛伐他汀对DR有预防和治疗作用。","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"155 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114207054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of CD4+ NK T cells on high-risk corneal transplantation in SEB-treated rat CD4+ NK T细胞在seb治疗大鼠高危角膜移植中的作用

Chinese Ophthalmic Research Pub Date : 2010-04-10 DOI: 10.3969/J.ISSN.1003-0808.2010.04.001

接英, 潘志强, 陈钰, 张文华, 武宇影

{"title":"The role of CD4+ NK T cells on high-risk corneal transplantation in SEB-treated rat","authors":"接英, 潘志强, 陈钰, 张文华, 武宇影","doi":"10.3969/J.ISSN.1003-0808.2010.04.001","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.04.001","url":null,"abstract":"目的研究超抗原金黄色葡萄球菌肠毒素B亚单位(SEB)治疗高危角膜移植免疫排斥反应与CD4+自然杀伤(NK)T细胞的相关性.方法 Fisher 344大鼠21只作为供体,Lewis大鼠42只作为受体.缝线法诱导角膜新生血管,建立高危角膜移植动物模型.将受体大鼠随机分为3组,每组14只.SEB组在角膜移植术前腹腔内注射75μg/kg SEB 0.2mL,每4日1次,共3次;SEB联合地塞米松组除按上述方法注射SEB外,在角膜移植术后每日结膜下注射5g/L地塞米松0.1mL,每日1次,共3次;生理盐水组按SEB组的方法腹腔内注射等体积生理盐水.术后观察植片混浊、水肿和新生血管指标并进行评分.术后第10天,每组取3只受体大鼠进行组织病理学和免疫学检测.结果 SEB组植片平均存活时间为(12.50±1.41)d,较SEB联合地塞米松组(10.38±3.07)d和生理盐水组(7.30±0.67)d明显延长(P＜0.05).SEB组淋巴细胞的增生能力明显降低,脾脏和颌下淋巴结中的CD4+ NK T细胞百分数明显升高.SEB联合地塞米松组脾脏和颌下淋巴结中的CD4+和CD8+细胞百分数均明显降低.SEB组房水和血清中IL-2质量浓度均明显降低,而IL-10质量浓度均明显升高.结论超抗原SEB能够明显延长大鼠高危角膜移植手术植片的存活时间,其作用机制与上调了CD4+NK T细胞有关,CD4+NK T细胞对于免疫耐受的形成有重要作用。","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"69 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127555549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0