Chinese Ophthalmic Research最新文献

{"title":"Treatment of experimental autoimmune uveitis in rats with arsenic trioxide","authors":"邹正平, 强孚勇, 张挺, 孙莉, 嘉婷, 朱小春, 徐宏","doi":"10.3969/J.ISSN.1003-0808.2010.04.005","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.04.005","url":null,"abstract":"目的 探讨三氧化二砷(As2O3)对实验性自身免疫性葡萄膜炎(EAU)大鼠模型的治疗作用.方法 将光感受器间维生素A类结合蛋白(IRBP)R14多肽免疫大鼠随机分为高剂量As2O3组、低剂量As2O3组、环孢素A(CsA)组和生理盐水组4组,每组5只,分别腹腔注射5mg/kg As2O3、0.5mg/kg As2O3、2mg/kg CsA及生理盐水0.2mL.裂隙灯显微镜观察大鼠眼前房炎症情况,第14天时处死大鼠,光学显微镜观察大鼠眼球的病理变化程度,免疫组织化学观察大鼠眼球中CD3+ T细胞的浸润情况,TUNEL法检测大鼠视网膜组织的凋亡.结果 生理盐水组和0.5mg/kg As2O3组可见明显的虹膜充血、血管扩张和脓性渗出,5mg/kg As2O3组和CsA组前房炎症反应程度明显较轻,与生理盐水组比较,5mg/kg As2O3组和CsA组的临床评分明显降低,差异均有统计学意义(tAs2O3 5mg=-0.656,P=0.042;tCsA =-1.308,P=0.002).组织病理学评分显示各组间差异有统计学意义(F=11.297,P=0.000).与生理盐水组比较,As2O3组和CsA组的CD3+T细胞浸润明显减少.TUNEL检测细胞凋亡在各组间差异无统计学意义(P＞0.05).结论 腹腔注射As2O3可以明显抑制EAU大鼠的临床表现、病理改变及CD3+T细胞的浸润,未增加病变部位细胞的凋亡。","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125102730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of melatonin on oxidative stress and the expression of ICAM-1 on retina in diabetic rat","authors":"吴昌凡, 薛嘉睿, 郎平","doi":"10.3969/J.ISSN.1003-0808.2010.04.010","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.04.010","url":null,"abstract":"目的 观察褪黑素对糖尿病(DM)大鼠氧化应激和视网膜组织中细胞间黏附分子-1(ICAM-1)表达的影响.方法 36只Wistar大鼠随机取10只作为正常对照组;其余26只制成DM模型,将造模成功的20只大鼠随机分为褪黑素组和DM组,每组10只.褪黑素组大鼠每日左下腹腔注射10mg/kg褪黑素进行干预治疗,正常对照组和DM组大鼠不进行治疗.12周后测各组大鼠的体重、血糖、血清一氧化氮(NO)及血清超氧化物歧化酶(SOD)值,并观察大鼠视网膜形态以及ICAM-1表达的不同.结果 12周后,与正常对照组比较,DM组和褪黑素组大鼠体重均明显下降(t=5.15,t=3.62;P＜0.05),血糖均升高(t=69.03,t=45.78;P＜0.05),而DM组和褪黑素组体重(t=1.88,P=0.09)和血糖(t=-1.41,P=0.19)比较,差异均无统计学意义.与正常对照组比较,DM组和褪黑素组大鼠血清中SOD、NO水平均明显下降(P＜0.05);与DM组比较,褪黑素组大鼠血清SOD值、血清NO水平均明显升高(t=9.45,t=2.11,P＜0.05).组织病理学检查显示,DM组和褪黑素组大鼠视网膜结构紊乱,白细胞黏附于血管内皮;免疫组织化学检测显示2组大鼠视网膜标本中ICAM-1颗粒表达于视网膜血管内皮细胞,但褪黑素组较DM组表达明显降低.结论 褪黑素可显著提高DM大鼠体内抗氧化能力、降低炎症反应,对DM引起的视网膜损伤有一定的保护作用。","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"348 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123328206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of mfERG in severe nonproliferative diabetic retinopathy","authors":"杜蓓, 徐延山, 张红","doi":"10.3969/J.ISSN.1003-0808.2010.04.021","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.04.021","url":null,"abstract":"目的 研究重度非增生型糖尿病视网膜病变(NPDR)多焦视网膜电图(mfERG)的特征及临床意义.方法 30例(40眼)重度NPDR患者为NPDR组和35例(35眼)正常人为对照组.以国际分期作为NPDR诊断纳入标准,mfERG记录过程遵循国际临床视觉电生理学会的标准化方案,每个受试者在接受检查前均取得知情同意.结果 与对照组相比,NPDR组患者mfERG2～5环的P1波、N1波反应密度明显下降,差异均有统计学意义(P＜0.05～0.01);mfERG第Ⅱ象限和第Ⅲ象限的P1波、N1波反应密度明显降低,差异均有统计学意义(P＜0.05～0.01).NPDR患者mfERG3～5环P1波、N1波隐含时较对照组明显延长,差异均有统计学意义(P＜0.05～0.01);第Ⅰ象限和第Ⅲ象限隐含时显著延迟,差异均有统计学意义(P＜0.05～0.01).结论 NPDR可导致视网膜黄斑区视功能的损伤,mfERG能够客观、定量地反映黄斑区功能损害的程度。","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129714825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The initial application of magnetic resonance diffusion tensor imaging in ischemic optic neuropathy","authors":"祁佩红, 史大鹏, 李四保, 樊红光, 轩昂, 郭希让, 程志兴","doi":"10.3969/J.ISSN.1003-0808.2010.04.012","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.04.012","url":null,"abstract":"目的 初步探讨磁共振扩散张量成像(MR-DTI)在缺血性视神经病变的检测价值.方法 对18例单侧缺血性视神经病变和20例健康成年人分别行3.0 MR-DTI检测,研究缺血视神经、健侧及对照组视神经的各向异性(FA)、表现扩散系数(ADC)、Trace W、E1、E2、相对各向异性指数(RA)、容积率(VR)值,并进行比较.结果 缺血性视神经病变患者FA、ADC、Trace W、E1、E2、RA、VR值分别为0.259±0.057、(1510±85)×10-6mm2/s、52±10、(1884±139)×10-6mm2/s、(1476±302)×10-6mm2/s、0.216±0.049、0.919±0.023.健侧视神经相应DTI参数值分别为0.574±0.018、(1025±67)×10-6mm2/s、67±8、(1661±103)×10-6mm2/s、(962±182)×10-6mm2/s、0.548±0.026、0.624±0.030.正常成人视神经相应DTI参数值分别为0.585±0.020、(935±133)×10-6mm2/s、69±15、(1654±157)×10-6mm2/s、(752±118)×10-6mm2/s、0.561±0.026、0.617±0.048.患侧视神经与健侧、对照组视神经相比,ADC、E2、VR值升高,FA值、RA值均降低,差异有统计学意义(P＜0.01).患侧视神经平均Trace W值与健侧(P=0.003)、对照组视神经(P=0.005)相比均升高,差异有统计学意义.患侧视神经E1值较健侧、对照组视神经升高,差异均有统计学意义(P=0.001).结论 MR-DTI可以作为缺血性视神经病变敏感、可靠的检测方法 。","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134349528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"眼睑黏膜相关淋巴瘤1例","authors":"陈海月, 金仁顺","doi":"10.3969/J.ISSN.1003-0808.2010.04.013","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.04.013","url":null,"abstract":"黏膜相关淋巴瘤(mucosa-associated lymphoid tumors,MALT)属于非霍奇金淋巴瘤中的一种亚型,其中原发于胃肠道者占51%;其他占49%,其中眼眶原发者占25%[1],眼睑MALT较少,现将来我院就诊的眼睑MALT 1例报告如下。","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"198200 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122057944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"外伤性开角型青光眼术后恶性青光眼1例","authors":"任晓冬, 刘伟, 魏玉斌, 田春玲","doi":"10.3969/J.ISSN.1003-0808.2010.04.015","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.04.015","url":null,"abstract":"患者,男,47岁,因右眼红肿、疼痛,伴剧烈头痛2d,于2009年7月9日入院.患者11年前右眼曾被打伤,在当地医院诊为右眼球顿挫伤、右眼视神经挫伤,治疗3个月,出院时右眼视力手动/眼前.入院检查:右眼视力光感,光定位:正上(+),颞上(+),余各方位(-);左眼视力1.0.右眼结膜充血,角膜哈气样水肿,前房深,虹膜纹理欠清,颞上虹膜根部可见少许新生血管,瞳孔4.5mm,对光反射消失,晶状体透明,眼底模糊,视盘苍白;左眼正常.右眼眼压66.23mmHg(1mmHg=0.133kPa),左眼眼压20.55mmHg。","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130335367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The current treatment of refractory glaucoma","authors":"朱勤, 胡竹林","doi":"10.3969/J.ISSN.1003-0808.2010.04.022","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.04.022","url":null,"abstract":"难治性青光眼是眼科常见病,常见病因包括多次滤过手术失败、新生血管性青光眼、角膜移植术后继发性青光眼等.因其病因复杂,病情多变,治疗手段虽较多,但疗效较差,常因药物耐受、滤过术后瘢痕阻塞通道等导致手术失败.针对难治性青光眼的特征,一些改良术式及联合术式逐渐应用,如复合式小梁切除术、脉络膜上腔引流术、房角金环植入术及全视网膜光凝联合avastin球内注射等.就难治性青光眼的各种治疗方法 、临床疗效及术后并发症等进行综述。","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123448958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short-term influence of LASIK on anterior and posterior corneal asphericity","authors":"J. Hou, Yan Wang, T. Zuo, Wei-li Geng, Ying Jin, Xiaoyan Yang, Li-qing Liu","doi":"10.3969/J.ISSN.1003-0808.2010.03.019","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.03.019","url":null,"abstract":"Background Laser in situ keratomileusis (LASIK) is frequently performed to reduce or eliminate myopic refractive error.Some patients complain of a loss of visual function after surgery even when they have 20/20 visual acuity.One of the reasons is the change of asphericity of the cornea.Objective This study attempts to investigate the short-term changes of the anterior and posterior corneal asphericity after LASIK.Methods One hundred and seven myopic eyes of 54 subjects with the equivalent spherical diopter of -1.30 to -7.50 D who have received LASIK were enrolled in this prospective study.The Q-values of the posterior corneal surface for different corneal diameters (6mm,7mm,8mm and 9mm) were measured with Pentacam preoperatively and 1 month postoperatively.The correlations between Q-value,Q change (△Q),and the mean preoperative spherical equivalent refraction (SE),central corneal thickness (CCT),central ablation depth (AD) and residual bed thickness were investigated.Written informed consents were obtained from all the subjects prior to the clinical trial.Results The Q-values of the anterior and posterior corneal surfaces gradually decreased to negative values with the increase of corneal diameter in myopic eyes.Weak correlations were found between the asphericity of the anterior and posterior corneal surfaces from diameters of 6mm,7mm,8mm and 9mm (r=0.227,0.288,0.303,0.389;P=0.019,0.003,0.002 and 0.000,respectively).No statistically significant correlation was found between Q-value and the diopter of refractive error (P＞0.05).Both the anterior and posterior corneal Q-values varied toward the positive direction except that in the 9mm area of the posterior corneal surface at postoperative 1 month(t=-1.495,P=0.138).The increase of the anterior corneal asphericity (△Q) was more obvious than that of the posterior corneal surface and showed a positive correlation with ablation depth and a negative correlation with residual bed thickness.However,no statistically significant correlation was seen between △Q and these two parameters in the posterior corneal surface (P＞0.05).Conclusion The shape of the anterior and posterior corneal surface shows more prolateness as the increase of corneal diameter in myopic eyes.Both the anterior and the posterior corneal surfaces have an oblate shift within the ablation zone 1 month after LASIK. \u0000 \u0000Key words: \u0000myopia; excimer laser; asphericity; Q-value","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131477946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of plasmin Kringle domains deficiency mutant on the interface of vitreous and retina in rabbit eyes","authors":"Wu Chen, Xin Huang, W. Mo, Wen-ji Wang, Hou-yan Song","doi":"10.3969/J.ISSN.1003-0808.2010.03.012","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.03.012","url":null,"abstract":"Background The vitreoretinal traction plays a critical role in the formation of macular hole and cystoid macular edema.Enzymatic vitreolysis has potential in relieving vitreoretinal traction as a simple and less invasive method in comparison with pars plane vitrectomy.ObjectiveThis study is to investigate the effects of plasmin mutant with kringle domains deficiency(Plm△K)on vitreoretinal interface in new Zealand white rabbits.Methods Plm△K was prepared through activating plasminogen mutant with Kringle domains deficiency (Plg△K) by tissue plasminogen activator (tPA).100μL of Plm△K at the dose of 0.5,1.0 and 1.5μmol/min was injected respectively into the vitreous of 48 New Zealand white rabbits and 16 eyes for each dose.B-scan and optical coherence tomography (OCT) were performed to detect the structure variety at the vitreoretinal interface in 1 day and 7 days after injection.The gross anatomy analysis with triamcinolone acetonide fine particle suspension,as well as histopathological examinations by scanning electron microscopy,was performed in the different time points mentioned above.Results Two peptide chains were determined with the relative molecular weight about 26000 and 5000 by the gel imaging analysis of reduced SDS-PAGE.Separation of the posterior vitreous cortex from retina was found after intravitreous injection under the B-scan and OCT.The ultrastructure change of vitreoretinal interface as well as the examination of fine particle suspension by triamcinolone acetonide demonstrated the same outcome.The degree of remnants of vitreous cortex showed the negative correlation with the dosage of Plm△K (r=-0.9516,P=0.048).No significant correlation was found between the degree of remnants of vitreous cortex and the action time(r=-0.720,P=0.470).There was no obvious morphological difference in outer layer of retina between control eyes and Plm△K-treated eyes.No drug-related adverse event was found after intravitreous injection of Plm△K.Conclusion Intravitreous injection of Plm△K alone can induce complete separation of vitreous from retina.This procedure is safe and effective. \u0000 \u0000Key words: \u0000vitreoretinal interface; plasmin; Kringle domain; deficiency mutant","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"389 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131753429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of hypoxia on the expression of AQP-4 in Miiller cells","authors":"Miao Zeng, Yang Cheng, ZengShuiqing","doi":"10.3969/CMA.J.ISSN.1003-0808.2010.03.014","DOIUrl":"https://doi.org/10.3969/CMA.J.ISSN.1003-0808.2010.03.014","url":null,"abstract":"Background Hypoxia is an important cause resulting in many retinal diseases,such as retinal edema,diabetic retinopathy,proliferative retinopathy and so on. Objective This study is to investigate the effects of hypoxia on the expression of AQP-4 in Muller ceils in vitro. Methods Muller cells were isolated from New Zealand white rabbits and primarily cultured in DMEM containing 20% fetal bovine serum by the explant culture method. The cells were identified by immunostaining for the glial fibrillary acidic protein（ GFAP）. Generation 2 of cells was cultivated with the chemical hypoxia inducer, CoCl2 ,for 24 hours in the hypoxic group and only with DMEM in the control group. The expression of the AQP-4 protein in Mtiller cells was detected by immunocytochemistry. The expression of AQP-4 mRNA was determined by semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR）. Results About 90% of Mtiller cells （generation 2） presented a positive immunoreactivity for GFAP, showing a brown staining in the cytoplasm. Cultured cells displayed the presence of intermediate filaments, microvillus and various cellular organs. The Integral absorbanee of the AQP-4 protein in Mtiller cells was markedly increased 24 hours after incubation with CoC12 in comparison with the control group （t = 6.74 ,P 〈 0.05 ）. The expression level of AQP-4 mRNA in MUller cells was significantly enhanced 24 hours after incubation with CoC12 in comparison with the control group （t = 21.79, P 〈 0.05 ）. Conclusion Hypoxia enchances the expression of AQP-4 in Mutiller cells and further increases fluid accumulation in the retina. These results suggest that Mtiller cells play an important role in the formation of retinal edema in diabetic retinopathy or proliferative retinopathy. \u0000 \u0000Key words: \u0000aquaporin-4 ;  Muller cell;  retinal edema;  hypoxia","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"113976572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}