Microscopy and Microanalysis最新文献_第5页

Suppressing Lithium Migration in a Carbon Fiber Negative Electrode During Atom Probe Tomography Analysis. 在原子探针断层扫描分析过程中抑制碳纤维负电极中的锂迁移。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-07-19 DOI: 10.1093/mam/ozae058

Marcus Johansen, Mahander P Singh, Baptiste Gault, Fang Liu

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Atom Probe Tomography Investigation of Clustering in Model P2O5-Doped Borosilicate Glasses for Nuclear Waste Vitrification. 用于核废料玻璃化的掺杂 P2O5 的硼硅玻璃模型中的聚类的原子探针断层扫描研究。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-07-08 DOI: 10.1093/mam/ozae059

Przemyslaw Klupś, Katrina Skerratt-Love, Albert A Kruger, Jaime George, Anthony Martin Thomas Bell, Paul A Bingham, Michael P Moody, Paul A J Bagot

{"title":"Atom Probe Tomography Investigation of Clustering in Model P2O5-Doped Borosilicate Glasses for Nuclear Waste Vitrification.","authors":"Przemyslaw Klupś, Katrina Skerratt-Love, Albert A Kruger, Jaime George, Anthony Martin Thomas Bell, Paul A Bingham, Michael P Moody, Paul A J Bagot","doi":"10.1093/mam/ozae059","DOIUrl":"https://doi.org/10.1093/mam/ozae059","url":null,"abstract":"Atom probe tomography (APT) has been utilized to investigate the microstructure of two model borosilicate glasses designed to understand the solubility limits of phosphorous pentoxide (P2O5). This component is found in certain high-level radioactive defence wastes destined for vitrification, where phase separation can potentially lead to a number of issues relating to the processing of the glass and its long-term chemical and structural stability. The development of suitable focused ion beam (FIB)-preparation routes and APT analysis conditions were initially determined for the model glasses, before examining their detailed microstructures. In a 3.0 mol% P2O5-doped glass, both visual inspection and sensitive statistical analysis of the APT data show homogeneous microstructures, while raising the content to 4.0 mol% initiates the formation of phosphorus-enriched nanoscale precipitates. This study confirms the expected inhomogeneities and phase separation of these glasses and offers routes to characterizing these at near-atomic scale resolution using APT.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141555179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Correction to: Low-Temperature X-ray Microanalysis Sheds New Light on Mineral Nutrition Aspects of Insect Leaf Galling. 更正：低温 X 射线显微分析为昆虫叶瘿病的矿物质营养问题提供了新线索

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-07-04 DOI: 10.1093/mam/ozae030

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Expression Patterns of Grainyhead-Like 2 and Ovo-Like 2 in Mouse Mammary Gland Development During Pregnancy, Lactation, and Weaning. Grainyhead-Like 2 和 Ovo-Like 2 在妊娠期、哺乳期和断奶期小鼠乳腺发育中的表达模式

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-07-04 DOI: 10.1093/mam/ozae049

Mohamed Elfeky, Shinya Matsuoka, Izumi Yamamoto, Yaser Hosny Ali Elewa, Kazuki Nakamura, Hiroyoshi Suzuki, Akihiro Kamikawa, Yuko Okamatu-Ogura, Kazuhiro Kimura

{"title":"Expression Patterns of Grainyhead-Like 2 and Ovo-Like 2 in Mouse Mammary Gland Development During Pregnancy, Lactation, and Weaning.","authors":"Mohamed Elfeky, Shinya Matsuoka, Izumi Yamamoto, Yaser Hosny Ali Elewa, Kazuki Nakamura, Hiroyoshi Suzuki, Akihiro Kamikawa, Yuko Okamatu-Ogura, Kazuhiro Kimura","doi":"10.1093/mam/ozae049","DOIUrl":"10.1093/mam/ozae049","url":null,"abstract":"Grainyhead-like 2 (Grhl2) is a transcription factor that regulates cell adhesion genes in mammary ductal development and serves as a repressor of the epithelial-mesenchymal transition. Conversely, Ovo-like2 (Ovol2) is a target gene of Grhl2 but functions as a substitute in Grhl2-deficient mice, facilitating successful epithelial barrier formation and lumen expansion in kidney-collecting ductal epithelial cells. Our objective was to examine the expression patterns of Grhl2, Ovol2, and their associated genes during the intricate phases of mouse mammary gland development. The mRNA expression of Grhl2 and Ovol2 increased after pregnancy. We observed Grhl2 protein presence in the epithelial cell's region, coinciding with acini formation, and its signal significantly correlated with E-cadherin (Cdh1) expression. However, Ovol2 was present in the epithelial region without a correlation with Cdh1. Similarly, Zeb1, a mesenchymal transcription factor, showed Cdh1-independent expression. Subsequently, we explored the interaction between Rab25, a small G protein, and Grhl2/Ovol2. The expressions of Grhl2 and Ovol2 exhibited a strong correlation with Rab25 and claudin-4, a tight junction protein. These findings suggest that Grhl2 and Ovol2 may collaborate to regulate genes associated with cell adhesion and are crucial for maintaining epithelial integrity during the different phases of mammary gland development.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141248362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Evaluating the Quality of Serial EM Sections with Deep Learning. 利用深度学习评估串行电磁截面的质量。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-07-04 DOI: 10.1093/mam/ozae033

Mahsa Bank Tavakoli, Josh L Morgan

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Low-Temperature X-ray Microanalysis Sheds New Light on Mineral Nutrition Aspects of Insect Leaf Galling. 低温 X 射线显微分析揭示了昆虫叶瘿病的矿物质营养新奥秘

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-07-04 DOI: 10.1093/micmic/ozae008

Denise R Fernando, Peter T Green, Alan T Marshall

{"title":"Low-Temperature X-ray Microanalysis Sheds New Light on Mineral Nutrition Aspects of Insect Leaf Galling.","authors":"Denise R Fernando, Peter T Green, Alan T Marshall","doi":"10.1093/micmic/ozae008","DOIUrl":"10.1093/micmic/ozae008","url":null,"abstract":"Manipulation of host plant physiology by leaf-galling insects is a multifaceted process. Among fundamental knowledge gaps surrounding this scientifically intriguing phenomenon is the appropriation of plant mineral nutrients and moisture for galling advantage. Small, soluble mineral ions and watery cell contents in dense gall tissues risk disruption during routine sample preparations. In this study, an X-ray microanalysis was applied to investigate gall mineral nutrition. Morphologically diverse leaf galls were sampled from three Australian rainforest tree species. Using cryo-analytical scanning electron microscopy, real-time X-ray analytical maps of cellular mineral nutrients and water were integrated with anatomical images of gall and leaf cross-sectional surfaces. A comparison of host-leaf and gall anatomies bore direct evidence of drastic changes to leaf cells through the galling process. Distinct \"wet\" and \"dry\" regions within galls were anatomically and/or chemically differentiated, suggesting specific functionality. \"Wet\" regions comprising hydrated cells including soft gall-cavity linings where larvae are known to feed contained soluble plant mineral nutrients, while C-rich \"dry\" tissues largely devoid of mineral nutrients likely contribute structural support. Mapping immobile nutrients such as Mn may provide a means of \"matching\" specific gall cell types to those in ungalled host-leaf tissues. The findings here provided otherwise inaccessible insights into leaf-gall mineral nutrition.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139697837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Postprocessing Workflow for Laboratory Diffraction Contrast Tomography: A Case Study on Chromite Geomaterials. 实验室衍射对比断层扫描的后处理工作流程：铬铁矿地质材料案例研究。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-07-04 DOI: 10.1093/mam/ozae036

Xiao Chen, Belinda Godel, Michael Verrall

{"title":"Postprocessing Workflow for Laboratory Diffraction Contrast Tomography: A Case Study on Chromite Geomaterials.","authors":"Xiao Chen, Belinda Godel, Michael Verrall","doi":"10.1093/mam/ozae036","DOIUrl":"10.1093/mam/ozae036","url":null,"abstract":"Texture stands as a fundamental descriptor in the realms of geology and earth and planetary science. Beyond offering insights into the geological processes underlying mineral formation, its characterization plays a pivotal role in advancing engineering applications, notably in mining, mineral processing, and metal extraction, by providing quantitative data for predictive modeling. Laboratory diffraction contrast tomography (LabDCT), a recently developed 3D characterization technique, offers nondestructive measurement of grain phases including their morphology, distribution, and crystal orientation. It has recently shown its potential to assess 3D textures in complex natural rock samples. This study looks at improving on previous work by examining the artifacts and presents a novel postprocessing workflow designed to correct them. The workflow is developed to rectify inaccurate grain boundaries and interpolate partially reconstructed grains to provide more accurate results and is illustrated using multi-scan examples on chromite sands and natural chromitite from the Upper Group 2 Reef layer in South Africa. The postcorrected LabDCT results were validated through qualitative and quantitative assessment using 2D electron back-scattered diffraction on polished sample surfaces. The successful implementation of this postprocessing workflow underscores its substantial potential in achieving precise textural characterization and will provide valuable insights for both earth science and engineering applications.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140864549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Buparvaquone Induces Ultrastructural and Physiological Alterations Leading to Mitochondrial Dysfunction and Caspase-Independent Apoptotic Cell Death in Leishmania donovani. 布帕伐醌诱导超微结构和生理变化，导致线粒体功能障碍和 Caspase 依赖性细胞凋亡。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-07-04 DOI: 10.1093/mam/ozae034

Swetapadma Majhi, Bhanu Priya Awasthi, Rakesh Kumar Sharma, Kalyan Mitra

{"title":"Buparvaquone Induces Ultrastructural and Physiological Alterations Leading to Mitochondrial Dysfunction and Caspase-Independent Apoptotic Cell Death in Leishmania donovani.","authors":"Swetapadma Majhi, Bhanu Priya Awasthi, Rakesh Kumar Sharma, Kalyan Mitra","doi":"10.1093/mam/ozae034","DOIUrl":"10.1093/mam/ozae034","url":null,"abstract":"Leishmaniasis is a neglected tropical disease (endemic in 99 countries) caused by parasitic protozoa of the genus Leishmania. As treatment options are limited, there is an unmet need for new drugs. The hydroxynaphthoquinone class of compounds demonstrates broad-spectrum activity against protozoan parasites. Buparvaquone (BPQ), a member of this class, is the only drug licensed for the treatment of theileriosis. BPQ has shown promising antileishmanial activity but its mode of action is largely unknown. The aim of this study was to evaluate the ultrastructural and physiological effects of BPQ for elucidating the mechanisms underlying the in vitro antiproliferative activity in Leishmania donovani. Transmission and scanning electron microscopy analyses of BPQ-treated parasites revealed ultrastructural effects characteristic of apoptosis-like cell death, which include alterations in the nucleus, mitochondrion, kinetoplast, flagella, and the flagellar pocket. Using flow cytometry, laser scanning confocal microscopy, and fluorometry, we found that BPQ induced caspase-independent apoptosis-like cell death by losing plasma membrane phospholipid asymmetry and cell cycle arrest at sub-G0/G1 phase. Depolarization of the mitochondrial membrane leads to the generation of oxidative stress and impaired ATP synthesis followed by disruption of intracellular calcium homeostasis. Collectively, these findings provide valuable mechanistic insights and demonstrate BPQ's potential for development as an antileishmanial agent.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140859735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Automating Wood Species Detection and Classification in Microscopic Images of Fibrous Materials with Deep Learning. 利用深度学习实现纤维材料显微图像中木材种类的自动检测和分类。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-07-04 DOI: 10.1093/mam/ozae038

Lars Nieradzik, Jördis Sieburg-Rockel, Stephanie Helmling, Janis Keuper, Thomas Weibel, Andrea Olbrich, Henrike Stephani

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Anatomical and Surface Electron Microscopic Investigation of the Tongue and Laryngeal Prominence in the Red-Eyed Turtle Dove (Streptopelia semitorquata, Rüppel 1837). 红眼斑鸠（Streptopelia semitorquata, Rüppel 1837）舌头和喉突的解剖学和表面电子显微镜研究。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-07-04 DOI: 10.1093/mam/ozae045

Casmir Onwuaso Igbokwe, Felix Ejikeme Mbajiorgu, Umar Muhammed Bello

{"title":"Anatomical and Surface Electron Microscopic Investigation of the Tongue and Laryngeal Prominence in the Red-Eyed Turtle Dove (Streptopelia semitorquata, Rüppel 1837).","authors":"Casmir Onwuaso Igbokwe, Felix Ejikeme Mbajiorgu, Umar Muhammed Bello","doi":"10.1093/mam/ozae045","DOIUrl":"10.1093/mam/ozae045","url":null,"abstract":"This study investigated the morphology of tongue and laryngeal structures in red-eyed dove and related it to feeding habits. Samples were examined using gross anatomy, scanning electron microscopy (SEM), and histological techniques. The tongue conformed to the shape of the lower beak, and the apex with a pointed tip, body, and root were distinguished. A median grove was apparent, and a papillary crest with pointed triangular papillae separated the body from the root. The length of the tongue, its width (body), and the percentage of the length of the lower jaw occupied by the tongue were, on average, 12.5 mm, 2.3 mm, and 57.3%, respectively. SEM showed highly desquamated dorsal epithelium with pointed papillae-like projections. The papillary crest presented pointed papillae of about 20-22. Salivary openings were apparent in the root, surrounded by mucosal folds and blunt papillae. Laryngeal prominence was irregularly triangular shaped and elevated. The rim of the glottis showed glandular pinpointed marks. Typical cornified multilayered mucosal epithelium was present in the apex and decreased caudally. Hyaline entoglossal cartilage and adjoining connective tissue were present in the body and apex. Numerous tubuloalveolar glands were seen in the body and root. The rim of the glottis showed mucous intraepithelial glands. The rostral and caudal lingual glands indicated positive reaction to neutral and acidic mucins. This study demonstrated a morphofunctional relationship of the tongue of a red-eyed dove to its diet.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141437204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0