Microscopy and Microanalysis最新文献

Multi-laboratory Validation of Join of the Five Dyes Revealing Collagenous Tissue (JFRL) Staining for Bone Histology Across Animal Species and Bone Implant Materials. 五种显示胶原组织的染料（JFRL）在不同动物种类和骨植入材料中的骨组织染色的多实验室验证。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-07-15 DOI: 10.1093/mam/ozaf058

Erika Tsuji, Kanako Sumi, Natsuko Tsuzuki, Daisuke Kondoh, Masashi Tsujio, Marina Hosotani, Takashi Namba, Shoichi Wakitani, Osamu Ichii, Ko Nakanishi, Teppei Nakamura

{"title":"Multi-laboratory Validation of Join of the Five Dyes Revealing Collagenous Tissue (JFRL) Staining for Bone Histology Across Animal Species and Bone Implant Materials.","authors":"Erika Tsuji, Kanako Sumi, Natsuko Tsuzuki, Daisuke Kondoh, Masashi Tsujio, Marina Hosotani, Takashi Namba, Shoichi Wakitani, Osamu Ichii, Ko Nakanishi, Teppei Nakamura","doi":"10.1093/mam/ozaf058","DOIUrl":"https://doi.org/10.1093/mam/ozaf058","url":null,"abstract":"Histological staining is essential for understanding bone structure and pathology; however, variations in decalcification agents can compromise reproducibility. We have previously developed a novel osteochondral staining method, Join of the Five dyes Revealing coLlagenous tissue (JFRL) staining, that is independent of the decalcification method. To promote its widespread adoption, this study confirms the robustness of JFRL staining through intra- and inter-laboratory validation. JFRL staining demonstrated consistent patterns across different manufacturers and facilities, with proper dehydration steps being crucial for optimal results. We applied JFRL staining to diverse vertebrate species prepared under various fixation and decalcification conditions to effectively visualize species-specific bone structures, including distinct osteoid and mineralized bone features from fish to large mammals. Furthermore, JFRL staining proved useful in evaluating bone biomaterials within defect models and clearly depicts the complex architecture of bone-healing processes and material integration. The staining qualitatively distinguished osteoid, mineralized bone, hyaline cartilage, and bone cells of different colors across all applications. These findings establish JFRL staining as a robust and versatile method for bone histology. Future studies focusing on quantitative assessment and pathological applications will prove that JFRL staining presents a reliable tool for both basic research and clinical diagnostics of bone disorders.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 4","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancing Cross-Sectional Scanning Electron Microscopy of Perovskite Solar Cells. 钙钛矿太阳能电池的截面扫描电镜研究进展。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-07-15 DOI: 10.1093/mam/ozaf067

Abigail Carbone, Robert Sinclair, Reinhold H Dauskardt

引用次数: 0

Ameliorative Potential of Mesenchymal Stem Cells-derived Exosomes on the Cerebellar Cortex in a Rat Model of Parkinson's Disease: Targeting HOTAIR/miRNA-221 Signaling Axis: A Histological, Immunohistochemical, and Biochemical Study. 针对HOTAIR/miRNA-221信号轴的间质干细胞衍生外泌体在帕金森病大鼠模型小脑皮层的改善潜力：组织学、免疫组织化学和生化研究

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-07-15 DOI: 10.1093/mam/ozaf062

Amany Mohamed Shalaby, Nema Soliman, Amira Mostafa Elshamy, Sulaiman Mohammed Alnasser, Mohammed Alorini, Hamad Alsaykhan, Fatima A Jaber, Mohamed Ali Alabiad, Amr Mohamed Younes, Mohamed Mahmoud Abdelrahim Elshaer, Walaa E Omar, Hanim Magdy Abdelnour

{"title":"Ameliorative Potential of Mesenchymal Stem Cells-derived Exosomes on the Cerebellar Cortex in a Rat Model of Parkinson's Disease: Targeting HOTAIR/miRNA-221 Signaling Axis: A Histological, Immunohistochemical, and Biochemical Study.","authors":"Amany Mohamed Shalaby, Nema Soliman, Amira Mostafa Elshamy, Sulaiman Mohammed Alnasser, Mohammed Alorini, Hamad Alsaykhan, Fatima A Jaber, Mohamed Ali Alabiad, Amr Mohamed Younes, Mohamed Mahmoud Abdelrahim Elshaer, Walaa E Omar, Hanim Magdy Abdelnour","doi":"10.1093/mam/ozaf062","DOIUrl":"https://doi.org/10.1093/mam/ozaf062","url":null,"abstract":"Among neurodegenerative diseases, Parkinson's disease (PD) is the second most common disorder. It is marked by the degeneration of dopaminergic neurons and depletion of dopamine. The mesenchymal stem cells (MSCs)-derived exosomes hold a promise for addressing neurodegeneration-associated neurological disorders owing to their distinctive immunomodulatory and regenerative properties. The investigation explored the therapeutic potential of MSCs-derived exosomes to mitigate the pathological changes in the cerebellar cortex in a rat model of PD. Thirty rats were divided into control, PD, and PD-BM-MSCs-derived exosomes groups. For 5 weeks, rodents were administered a subcutaneous injection of 2 mg/kg/day of rotenone to induce a PD model. The PD group exhibited a substantial increase in relative cerebellar mRNA HOTAIR, BAX, and caspase 3 gene expression, along with a concomitant decrease in relative cerebellar miRNA-221 gene expression. Light and transmission electron microscopy also depicted marked degenerative changes in the cerebellar cortex. The immune expression of glial fibrillary acidic protein and ionized calcium-binding adaptor molecule-1 markedly increased, while synaptophysin expression markedly decreased. Interestingly, all changes showed a significant regression following treatment with exosomes derived from BM-MSCs. In conclusion, BM-MSCs-derived exosomes may be a promising PD intervention strategy.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 4","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlative Imaging of Structural Biochemistry in Plant and Food Quality Research Within an Interoperable Data Acquisition Platform. 基于互操作数据采集平台的植物结构生物化学相关成像与食品质量研究

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-07-15 DOI: 10.1093/mam/ozaf057

Marjana Regvar, Boštjan Jenčič, Martin Šala, Aleš Kladnik, Iztok Dogša, Maja Koblar, Mitja Kelemen, Primož Vavpetič, Katarina Vogel-Mikuš, Ivan Kreft, Primož Pelicon, Paula Pongrac

{"title":"Correlative Imaging of Structural Biochemistry in Plant and Food Quality Research Within an Interoperable Data Acquisition Platform.","authors":"Marjana Regvar, Boštjan Jenčič, Martin Šala, Aleš Kladnik, Iztok Dogša, Maja Koblar, Mitja Kelemen, Primož Vavpetič, Katarina Vogel-Mikuš, Ivan Kreft, Primož Pelicon, Paula Pongrac","doi":"10.1093/mam/ozaf057","DOIUrl":"https://doi.org/10.1093/mam/ozaf057","url":null,"abstract":"Correlative imaging is a powerful tool for revealing information on cell-type structures and their biochemistry, with the potential to inform healthier food choices and improved dietary recommendations. Determination of plant structures and their structural biochemistry advances our understanding of specific structures designed to store different biomolecules within cells and tissues. Compared to the classical biochemical separation techniques, the key advantage of sequential correlative imaging techniques is in relating spatial plant (micro)structures to their biochemistry in a nondestructive manner. Sequential imaging reported here comprises six methodologies on a single sample, a cross-section of a Tartary buckwheat (Fagopyrum tataricum) grain, namely, bright-field and autofluorescence microscopy, fluorescence microspectroscopy, MeV-secondary ion mass spectrometry, micro-particle-induced X-ray emission, scanning electron microscopy coupled with energy dispersive X-ray spectroscopy, and laser ablation-inductively coupled plasma-mass spectrometry. Results confirm that the stepwise addition of the desired information across several classes of biomolecules and several spatial scales informs the quality and safety of plant-based produce across scales. Therefore, a viable workflow is proposed, enabling sequential spatial analysis of grain and highlighting plant structures' in situ specificity. The advantages and disadvantages of the selected methodologies were critically evaluated.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 4","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mapping the Path to Cryogenic Atom Probe Tomography Analysis of Biomolecules. 绘制生物分子低温原子探针层析分析路径。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-07-15 DOI: 10.1093/mam/ozaf032

Eric V Woods, Tim M Schwarz, Mahander P Singh, Shuo Zhang, Se-Ho Kim, Ayman A El-Zoka, Lothar Gremer, Dieter Willbold, Ingrid McCarroll, Baptiste Gault

{"title":"Mapping the Path to Cryogenic Atom Probe Tomography Analysis of Biomolecules.","authors":"Eric V Woods, Tim M Schwarz, Mahander P Singh, Shuo Zhang, Se-Ho Kim, Ayman A El-Zoka, Lothar Gremer, Dieter Willbold, Ingrid McCarroll, Baptiste Gault","doi":"10.1093/mam/ozaf032","DOIUrl":"https://doi.org/10.1093/mam/ozaf032","url":null,"abstract":"The understanding of protein structure and interactions remains a fundamental challenge in modern biology. While X-ray and electron-based techniques have provided atomic-level protein configurations, they require numerous molecules for averaged views and lack detailed compositional information crucial for biochemical activity. Atom probe tomography (APT) emerges as a promising tool for biological material analysis, though its capabilities for examining biomolecules in their native, hydrated state remain largely unexplored. We present systematic analyses of amino acids in frozen aqueous solutions using two different nanoporous metal supports across various analysis conditions. Our methodology employs a complete cryogenic workflow, including drop-casting, inert gas glovebox freezing, and specimen transfer via a cryogenically cooled ultra-high vacuum shuttle to both focused ion beam microscopy and atom probes. Using water molecular ion ratios as electrostatic field condition indicators, we investigate amino acid fragmentation and behavior. We evaluate the critical factors for successful biomolecular analysis: support material selection, cryogenic specimen preparation, and optimal data acquisition parameters. This work establishes guidelines for cryogenic APT analysis of biomolecules, advancing the technique's application in biological sciences.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 4","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144708132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leucine-Rich Repeat Kinase 2 Regulates Mitochondria for Zygotic Genome Activation in Mouse Early Embryos. 富亮氨酸重复激酶2调控线粒体对小鼠早期胚胎合子基因组激活的影响。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-07-15 DOI: 10.1093/mam/ozaf037

Yu-Lan Lu, Zi-Yu Wei, Xiao-Ting Yu, Li-Zhou Qin, Lin-Lin Hu, Shao-Chen Sun, Shang-Lin Yang

{"title":"Leucine-Rich Repeat Kinase 2 Regulates Mitochondria for Zygotic Genome Activation in Mouse Early Embryos.","authors":"Yu-Lan Lu, Zi-Yu Wei, Xiao-Ting Yu, Li-Zhou Qin, Lin-Lin Hu, Shao-Chen Sun, Shang-Lin Yang","doi":"10.1093/mam/ozaf037","DOIUrl":"https://doi.org/10.1093/mam/ozaf037","url":null,"abstract":"Leucine-rich repeat kinase 2 (LRRK2) is a multidomain protein known for its involvement in neurodegenerative disorders, particularly Parkinson's disease, where it is considered one of the most common genetic contributors. LRRK2 plays multiple roles in cellular signaling, protein trafficking, and cytoskeletal dynamics. In present study, using mouse as the mammalian model, we reported its important roles in early embryo development. We showed that LRRK2 accumulated around nucleus before two-cell stage but distributed in the cytoplasm of blastomeres after four-cell stage. Loss of LRRK2 activity induced two-cell to four-cell transition defects, indicating the failure of zygotic genome activation during embryo development. We showed the mitochondria dysfunction after LRRK2 inhibition, since the mitochondria distribution, intensity, ATP production, and mitochondria number were all altered. This might further induce the evaluated ROS level for the occurrence of oxidative stress. Besides, we also observed that the cortex and cytoplasmic actin in the blastomere of embryos were decreased, which further linked with mitochondria. In summary, we showed that LRRK2 activity is essential for actin-based mitochondria distribution and function, which further controls the occurrence of oxidative stress for mouse early embryo development.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 4","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Precision Local Burnup Assessment Through Dynamic Peak Fitting in Atom Probe Tomography for Depleted, Enriched, and Irradiated Metallic and Ceramic Fuels. 通过原子探针断层扫描动态峰拟合对贫化、富集和辐照金属和陶瓷燃料进行精确的局部燃耗评估。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-07-15 DOI: 10.1093/mam/ozaf066

Anshul Kamboj, Sohail Shah, Joshua Kane, Jian Gan, William Hanson, Jefferey Giglio, Mukesh Bachhav

{"title":"Precision Local Burnup Assessment Through Dynamic Peak Fitting in Atom Probe Tomography for Depleted, Enriched, and Irradiated Metallic and Ceramic Fuels.","authors":"Anshul Kamboj, Sohail Shah, Joshua Kane, Jian Gan, William Hanson, Jefferey Giglio, Mukesh Bachhav","doi":"10.1093/mam/ozaf066","DOIUrl":"https://doi.org/10.1093/mam/ozaf066","url":null,"abstract":"Burnup estimation in nuclear fuels is vital for evaluating fuel performance, transportation, and safe fuel storage. Accurate assessments of burnup from service period and spent fuels involve tracking the consumption of fissile isotopes of uranium (U) offering a direct insight into energy changes within the fuels especially for thermal spectrum reactors. In current approach, mass spectroscopic technique in atom probe tomography (APT) is utilized for accurate quantification of U isotopes. Quantification of U peaks in mass spectrum is performed on asymmetric shapes due to delayed signals, known as thermal tails, particularly for poorly conducting samples analyzed in laser mode. In this study, we introduce a novel quantification tool for isotopic analysis from APT datasets by developing a fitting algorithm based on shapes of the peaks. A MATLAB-based dynamic peak fitting toolbox is developed and designed to adapt to various peak shapes, ensuring accurate quantification of U isotopes. The effectiveness of this approach is demonstrated in standard Ni-Cr sample, depleted and enriched U samples, and U-based fuels with different burnup levels. The viability of this approach for isotopic quantification is demonstrated on both metallic and ceramic fuels.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 4","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strategies for Preparing and Analyzing Thin Passive Films With Atom Probe Tomography. 用原子探针层析技术制备和分析钝化薄膜的策略。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-07-15 DOI: 10.1093/mam/ozaf023

Elizabeth J Kautz, Kayla H Yano, Josephine C Hartmann, Angela Y Gerard, Sandra D Taylor, John R Scully, Daniel K Schreiber

{"title":"Strategies for Preparing and Analyzing Thin Passive Films With Atom Probe Tomography.","authors":"Elizabeth J Kautz, Kayla H Yano, Josephine C Hartmann, Angela Y Gerard, Sandra D Taylor, John R Scully, Daniel K Schreiber","doi":"10.1093/mam/ozaf023","DOIUrl":"https://doi.org/10.1093/mam/ozaf023","url":null,"abstract":"Atom probe tomography (APT) provides a unique, three-dimensional map of elemental and isotopic distributions over a wide range of materials with near-atomic scale resolution and is particularly strong at analyzing buried interfaces within materials. However, it is much more difficult to apply atom probe to the analysis of nanoscale surface films, such as those formed during alloy passivation, where unique challenges persist for sample preparation and data collection. Here, we present sample preparation strategies involving the deposition of a <100 nm capping layer that enables reliable characterization of thin passive films ∼2-5 nm thick formed on binary and multiprincipal element alloys via APT. Several capping layer materials (Pt, Ti, and Ni/Cr bilayer) and deposition methods are contrasted. Our results indicate a sputtered Ni/Cr bilayer enables the characterization of the entire passive film and concentration profiles that can easily be interpreted to clearly distinguish base alloy/passive film/capping layer interfaces. Lastly, we highlight ongoing challenges and opportunities for this experimental approach.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 4","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144699012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding the Impact of Reperfusion in the Development of a Safe Compression Therapy. 了解再灌注对安全压迫治疗发展的影响。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-07-15 DOI: 10.1093/mam/ozaf056

Rachel Beatty, Pascal Paco Farina, Joanne O'Dwyer, Peter Dockery, Alanna Stanley

{"title":"Understanding the Impact of Reperfusion in the Development of a Safe Compression Therapy.","authors":"Rachel Beatty, Pascal Paco Farina, Joanne O'Dwyer, Peter Dockery, Alanna Stanley","doi":"10.1093/mam/ozaf056","DOIUrl":"https://doi.org/10.1093/mam/ozaf056","url":null,"abstract":"The benefits of an intermittent compression therapy are known; however, in some circumstances, it results in soft-tissue injuries. This study aims to create a model for testing the safety, efficacy, and molecular mechanisms of a compression therapy, without creating an injury. Existing ischemia/reperfusion protocols were modified to apply repeated compression without forming a pressure ulcer. The skin of immobilized C57BL/6 male mice was pinched between two magnets (3 h, 2-3 days of recovery between treatments). Hair growth was stunted but started to regrow within 18 days. The application of pressure caused an increase in epidermal thickness (****p = 0.002), CD31+ total number (**p = 0.0021), and increased radial diffusion distance (*p = 0.0285). Pressure caused a reduction in adipocyte volume weight mean volume (****p < 0.0001) and CD34 volume fraction (*p = 0.0460). Stem cell populations (cytokeratin 15; p = 0.4992, CD34; p = 0.6873 and integrin α-6; p = 0.7752) were unaffected. Importantly, pressure did not increase markers of DNA damage (p53; p = 0.7524 and caspase 3; p = 0.0839), indicating that this compression model had limited negative effects on the tissue. The altered protocol successfully created a model to permit the analysis of uninjured skin after pressure is applied.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 4","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Insights Into Egyptian Tortoise (Testudo kleinmanni) Tongue Morphology Using Immunofluorescence Analysis. 利用免疫荧光分析对埃及龟（Testudo kleinmanni）舌头形态的新见解。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-07-15 DOI: 10.1093/mam/ozaf064

Samir A A El-Gendy, Mohamed A M Alsafy, Ahmed A El-Mansi, Mamdouh B Eldesoqui, Ahmed M Rashwan

{"title":"Novel Insights Into Egyptian Tortoise (Testudo kleinmanni) Tongue Morphology Using Immunofluorescence Analysis.","authors":"Samir A A El-Gendy, Mohamed A M Alsafy, Ahmed A El-Mansi, Mamdouh B Eldesoqui, Ahmed M Rashwan","doi":"10.1093/mam/ozaf064","DOIUrl":"https://doi.org/10.1093/mam/ozaf064","url":null,"abstract":"The Egyptian tortoise (Testudo kleinmanni) exhibits remarkable adaptations; yet, significant gaps remain in the understanding of taste bud distribution and the molecular characteristics of its tongue cells. The unique adaptations were investigated using immunofluorescence with six specific antibodies, light microscopy, and scanning electron microscopy. Vimentin and nestin identified superficial taste buds in the lingual body, notably larger than those in the lingual root. The lingual root's fungiform papillae contained deep taste buds. Vimentin showed broad expression across all taste bud cells, while nestin specifically marked basal cells. Co-expression of platelet-derived growth factor receptor alpha (PDGFRα) and cluster of differentiation 34 indicated telocyte-like cells within the papillae. Circular and rectangular hyaline cartilage was also observed, and PDGFRα and SRY-box transcription factor 9 (SOX9) co-expression was demonstrated in chondrocytes, with SOX9 showing higher expression. Cluster of differentiation 19 also identified B-cell lymphocytes in the lingual root. This comprehensive study provides the first application of immunofluorescence to the Egyptian tortoise tongue. It represents new insights into the structure and molecular composition of taste buds of its tongue, highlights its role in physiological adaptation, and contributes to a better understanding of the biology species.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 4","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0