Microscopy and Microanalysis最新文献

Morphophysiological Aspects of the Muscle Injury Caused by Eccentric Exercise. 偏心运动引起肌肉损伤的形态生理学研究。

IF 3 4区工程技术

Microscopy and Microanalysis Pub Date : 2026-03-03 DOI: 10.1093/mam/ozag011

Mariana Baptista, Isabella Gomes, Matheus Bertanha Fior, Jurandyr Pimentel Neto, Valter Tadeu Boldarine, Lila Missae Oyama, Lara Caetano Rocha-Braga, Adriano Polican Ciena

{"title":"Morphophysiological Aspects of the Muscle Injury Caused by Eccentric Exercise.","authors":"Mariana Baptista, Isabella Gomes, Matheus Bertanha Fior, Jurandyr Pimentel Neto, Valter Tadeu Boldarine, Lila Missae Oyama, Lara Caetano Rocha-Braga, Adriano Polican Ciena","doi":"10.1093/mam/ozag011","DOIUrl":"10.1093/mam/ozag011","url":null,"abstract":"Muscle injuries impair function by damaging cells and triggering inflammation. Eccentric exercise is an effective model for studying these injuries as it overstretches muscle fibers and activates inflammatory responses. The aim of the study was to examine morphological, biochemical, and functional adaptations in the muscle after an eccentric exercise injury protocol. Forty male Wistar rats were divided into Control (C, n = 8) and Injury (I, n = 32) groups, split by recovery time. The injury protocol involved 18 sets/5 min on a treadmill at a -16° incline and 16 m/min. Functional assessments measured joint edema and grip strength. For light microscopy, we used Hematoxylin-Eosin and Picrosirius, and for protein analysis, we measured the levels of interleukin-6 (IL-6), IL-10, and tumor necrosis factor-alpha (TNF-α) via ELISA. Results showed Injury groups with reduced body and muscle mass, and grip strength. The PI0H group exhibited elevated TNF-α and IL-6, while PI24H had increased joint edema. The PI7D group showed an increase in connective tissue and joint circumference. In conclusion, the protocol causes an inflammatory response with increase in nuclei density, and in the area of connective tissue associated with functional decline, with regeneration starting with the presence of neutrophils in PI24H and macrophages in PI4D.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"32 2","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147348781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detective Quantum Efficiency-Based Comparison of HRTEM and Ptychography Phase Imaging. 基于探测量子效率的HRTEM与平面摄影相位成像的比较。

IF 3 4区工程技术

Microscopy and Microanalysis Pub Date : 2026-03-03 DOI: 10.1093/mam/ozag018

Felix Bennemann, Angus I Kirkland, David A Muller, Peter D Nellist

引用次数: 0

Contextual High-Throughput 3D Volume Electron Microscopy Data Acquisition Using Artificial Intelligence. 使用人工智能的上下文高通量3D体积电子显微镜数据采集。

IF 3 4区工程技术

Microscopy and Microanalysis Pub Date : 2026-03-03 DOI: 10.1093/mam/ozag014

Tereza Hurník Konečná, Radek Jančík, Daniela Slamková, Bronislav Přibyl, Cveta Tomova, Lolita Rotkina, Tessa Burch-Smith, Sanja Sviben, James A J Fitzpatrick, Kirk J Czymmek

{"title":"Contextual High-Throughput 3D Volume Electron Microscopy Data Acquisition Using Artificial Intelligence.","authors":"Tereza Hurník Konečná, Radek Jančík, Daniela Slamková, Bronislav Přibyl, Cveta Tomova, Lolita Rotkina, Tessa Burch-Smith, Sanja Sviben, James A J Fitzpatrick, Kirk J Czymmek","doi":"10.1093/mam/ozag014","DOIUrl":"https://doi.org/10.1093/mam/ozag014","url":null,"abstract":"One of the greatest challenges when generating large and/or high-resolution three-dimensional (3D) volume electron microscopy (vEM) datasets is long acquisition times. We developed a method that leverages Artificial intelligence algorithms to increase acquisition throughput for 3D datasets by creating an AI-derived mask for the targeted region of interest, referred to as Adaptive Scanning. This allowed for specific structures to be imaged at high resolution, with the surrounding area captured at lower resolution, without artificially generating or enhancing any raw data. We demonstrate that this dynamic-resolution scanning approach significantly reduced acquisition time depending on the number of pixels of interest and was sample agnostic, being compatible with a diverse array of organisms and tissues, including brains, parasites, cultured cells, and plants. This multiresolution strategy has the potential to enhance focused ion beam scanning electron microscopy and other forms of vEM, by increasing time savings by up to twofold or more, enabling routine generation of multiple and/or larger vEM datasets more efficiently, cost-effectively, and allowing more data collection to increase statistical power for comparative studies.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"32 2","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147458588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Label-Free Imaging of Red Blood Cells in Gestational Diabetes: Autofluorescence Captures Oxidative Stress Beyond Membrane Fluidity. 妊娠期糖尿病红细胞无标记成像：自身荧光捕捉膜流动性以外的氧化应激。

IF 3 4区工程技术

Microscopy and Microanalysis Pub Date : 2026-03-03 DOI: 10.1093/mam/ozag016

Alessia Riente, Caterina Neri, Duaa Hatem, Cassandra Serantoni, Michele Maria De Giulio, Davide Archelao Dessì, Davide Manieri, Alessandro Rizzi, Linda Tartaglione, Lorenzo Lucacchini Paoli, Marco De Spirito, Giuseppe Maulucci, Dario Pitocco

{"title":"Label-Free Imaging of Red Blood Cells in Gestational Diabetes: Autofluorescence Captures Oxidative Stress Beyond Membrane Fluidity.","authors":"Alessia Riente, Caterina Neri, Duaa Hatem, Cassandra Serantoni, Michele Maria De Giulio, Davide Archelao Dessì, Davide Manieri, Alessandro Rizzi, Linda Tartaglione, Lorenzo Lucacchini Paoli, Marco De Spirito, Giuseppe Maulucci, Dario Pitocco","doi":"10.1093/mam/ozag016","DOIUrl":"https://doi.org/10.1093/mam/ozag016","url":null,"abstract":"Gestational diabetes mellitus (GDM) is a transient pregnancy disorder marked by insulin resistance and oxidative stress. In type 1 and 2 diabetes, reduced red blood cell (RBC) membrane fluidity, measured by Laurdan-GP, associates with vascular risk, but its role in GDM is unclear. This study compared GP with intrinsic RBC autofluorescence (green-to-red ratio, AFG,R) to identify a better marker of metabolic stress in GDM. Forty-eight pregnant women were enrolled (31 GDM, 17 controls). High-resolution multispectral confocal imaging quantified RBC GP and AFG,R. A machine-learning pipeline analyzed core, membrane, and whole-cell metrics, alongside clinical and biochemical data. Logistic regression assessed associations with GDM. GP did not differ between groups (0.60 ± 0.04 versus 0.61 ± 0.04; p = 0.168), unlike the reductions reported in chronic diabetes. Conversely, AFG,R values were higher in GDM across compartments (core: -0.073 ± 0.202 versus -0.154 ± 0.060; membrane: 0.013 ± 0.198 versus -0.066 ± 0.075; p = 0.049). In multivariable analysis, AFG,R Core independently predicted GDM status (p = 0.018), outperforming clinical and hematological markers. In GDM, membrane fluidity remains unchanged, but RBC autofluorescence reflects acute oxidative stress, supporting its use as a non-invasive, repeatable complement to OGTT for early risk assessment and monitoring.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"32 2","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147481070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relaxing Direct Ptychography Sampling Requirements via Parallax Imaging Insights. 通过视差成像洞察放松直接平面摄影采样要求。

IF 3 4区工程技术

Microscopy and Microanalysis Pub Date : 2026-03-03 DOI: 10.1093/mam/ozaf139

Georgios Varnavides, Julie Marie Bekkevold, Stephanie M Ribet, Mary C Scott, Lewys Jones, Colin Ophus

引用次数: 0

Exploring Mechanisms Leading to Composition Errors in Monazite (CePO4) Analyzed With Atom Probe Tomography. 原子探针层析分析Monazite （CePO4）成分误差的机制

IF 3 4区工程技术

Microscopy and Microanalysis Pub Date : 2026-03-03 DOI: 10.1093/mam/ozag009

Tom Veret, Fabien Delaroche, Ivan Blum, Jonathan Houard, Benjamin Klaes, Isabelle Mouton, Frederic De-Geuser, Anne-Magali Seydoux-Guillaume, François Vurpillot

{"title":"Exploring Mechanisms Leading to Composition Errors in Monazite (CePO4) Analyzed With Atom Probe Tomography.","authors":"Tom Veret, Fabien Delaroche, Ivan Blum, Jonathan Houard, Benjamin Klaes, Isabelle Mouton, Frederic De-Geuser, Anne-Magali Seydoux-Guillaume, François Vurpillot","doi":"10.1093/mam/ozag009","DOIUrl":"https://doi.org/10.1093/mam/ozag009","url":null,"abstract":"Monazite (CePO4) is widely used in U-Th-Pb geochronology due to its reliable age determinations, although isotopic disturbances often require investigation at the nanoscale to better understand the mechanisms at play. Atom probe tomography (APT) offers unique capabilities for nanoscale chemical analysis and 3D atomic reconstruction but presents challenges for insulating materials such as CePO4, particularly due to oxygen loss during field evaporation. This study investigates the effects of laser wavelength, energy, metallic coatings, and detection device on mass spectrum optimization and compositional accuracy in synthetic CePO4 samples. Results show that shorter laser wavelengths (260 nm) enhance peak resolution, particularly when combined with advanced reflectron configurations, as demonstrated with the LEAP 6000 XR. Chromium coatings further improve thermal dissipation and reduce noise levels. However, compositional measurements reveal systematic underestimation of oxygen and overestimation of P and Ce, likely influenced by preferential low-field evaporation of certain elements. These findings highlight the need to carefully tune experimental parameters to mitigate quantification biases and enhance the reliability of APT analyses for geological materials.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"32 2","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147463490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Systematic Scanning Electron Microscopy Study on MoS2 Layers Using In-lens Detector and Everhart-Thornley Detector. 基于透镜内检测器和Everhart-Thornley检测器的二硫化钼层系统扫描电镜研究。

IF 3 4区工程技术

Microscopy and Microanalysis Pub Date : 2026-03-03 DOI: 10.1093/mam/ozag022

Yixin Liu, Chao Wang, Wanqing Chen, Li Huang

{"title":"A Systematic Scanning Electron Microscopy Study on MoS2 Layers Using In-lens Detector and Everhart-Thornley Detector.","authors":"Yixin Liu, Chao Wang, Wanqing Chen, Li Huang","doi":"10.1093/mam/ozag022","DOIUrl":"https://doi.org/10.1093/mam/ozag022","url":null,"abstract":"A systematic SEM imaging of partially covered CVD-grown monolayer MoS2 on SiO2/Si substrates and the exfoliated MoS2 on Si substrates by both the in-lens detector and the Everhart-Thornley detector under varying acceleration voltage and working distance has been demonstrated. Superior contrast of MoS2 layers can be feasibly realized with the in-lens detector even at high acceleration voltage of 10 kV and large working distance of 20 mm. In the in-lens images, MoS2 layers appear consistently darker than the bare SiO2/Si substrate, which is a nice example of mass-thickness contrast. For Everhart-Thornley images, a contrast reversal of MoS2 layers was observed. Specifically, at working distance≤10 mm, MoS2 layers are brighter than the bare SiO2/Si substrates, which belong to atomic number contrast; at working distance≥15 mm, MoS2 layers appear darker than the bare SiO2/Si substrates. The underlying mechanism of contrast reversal was revealed. The dependence of MoS2 contrast in in-lens images and Everhart-Thornley images on acceleration voltage and working distance have been discussed by analyzing different responses of three types SE. The findings reported here will have important implications for effective SEM characterizations of MoS2, and other transition metal dichalcogenides.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"32 2","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147574699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EDX Array Tomography: 3D Imaging With Chemical Sensitivity and Submicron Resolution Through Ultramicrotomy and SEM-EDX Mapping. EDX阵列层析成像：通过超显微切开术和SEM-EDX成像，具有化学灵敏度和亚微米分辨率的三维成像。

IF 3 4区工程技术

Microscopy and Microanalysis Pub Date : 2026-03-03 DOI: 10.1093/mam/ozag012

David McLaughlin, Markus Bierling, Colin P O'Brien, David Sinton, Simon Thiele, Thomas Böhm

{"title":"EDX Array Tomography: 3D Imaging With Chemical Sensitivity and Submicron Resolution Through Ultramicrotomy and SEM-EDX Mapping.","authors":"David McLaughlin, Markus Bierling, Colin P O'Brien, David Sinton, Simon Thiele, Thomas Böhm","doi":"10.1093/mam/ozag012","DOIUrl":"https://doi.org/10.1093/mam/ozag012","url":null,"abstract":"Elemental contrast from energy-dispersive X-ray spectroscopy (EDX) provides valuable insights into various materials and samples. However, this surface-mapping technique suffers from poor depth resolution, which prevents a straightforward application to tomographic reconstruction. Additionally, due to charge artifacts or beam damage, this technique is often limited to electrically conductive samples, which does not allow its use on multiphase samples with low conductivity and complex 3D geometries. We developed an adaptation of array tomography using an automated tape-collecting ultramicrotome (ATUMtome) and scanning electron microscopy with EDX mapping to enable tomographic imaging of polymer-containing multiphase samples at submicron resolution. Hence, this method overcomes the limited depth resolution of EDX mapping, and by surface-coating the sections before imaging, charging artifacts are mitigated. Recently, we demonstrated the utility of the technique by applying it to a multiphase microporous layer encountered in a CO2 electrolyzer gas diffusion electrode, which could not be imaged using conventional tomographic approaches. A digital model of the full electrode was obtained in combination with focused ion beam scanning electron microscopy tomography and X-ray microtomography. Here, we describe the process of EDX array tomography in detail and elucidate the advantages of the technique in the field of energy materials.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"32 2","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147675056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sequential Development of Albino Rabbit Iris During Fetal and Postnatal Life: Histological, Ultrastructural, and Immunohistochemical Investigations. 白化兔虹膜在胎儿和出生后的顺序发育：组织学、超微结构和免疫组织化学研究。

IF 3 4区工程技术

Microscopy and Microanalysis Pub Date : 2026-03-03 DOI: 10.1093/mam/ozag015

Nagwa Ibrahim, Sara M M Eldesoky

{"title":"Sequential Development of Albino Rabbit Iris During Fetal and Postnatal Life: Histological, Ultrastructural, and Immunohistochemical Investigations.","authors":"Nagwa Ibrahim, Sara M M Eldesoky","doi":"10.1093/mam/ozag015","DOIUrl":"https://doi.org/10.1093/mam/ozag015","url":null,"abstract":"The iris has several unique structures that are determined by its embryonic origin. Therefore, this research aimed to demonstrate the morphogenesis of the albino rabbit iris. The development of the rabbit iris began at mid-gestation and was completed postnatally. Consequently, we used 36 embryos and 12 neonatal rabbits. The iris originated from the tip of the optic cup. The epithelium of the iris began to differentiate on day 15 of pregnancy, forming a single layer of epithelial cells. The constrictor muscle began its differentiation on day 27 of pregnancy in the area around the pupil, while the dilator muscle differentiated on day 30 as a protrusion from the anterior epithelial cells. The stroma of the iris differentiated from the periocular mesenchyme into fibroblasts, telocytes, dendritic cells, and macrophages. The tyrosinase enzymes expressed in the albino rabbit's iris were inactive and at low levels, with melanocytes lacking melanin pigment. Vimentin immunohistochemistry expression was observed in all layers of the iris. Desmin was expressed only in the constrictor muscle and stroma. Explaining the developmental process of the albino's iris is vital for advancing our knowledge of this tissue.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"32 2","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147458608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chondrocyte Division in the Brachial Cartilage of Silver Carp: Atypical Cell Division. 鲢鱼肱软骨的软骨细胞分裂：非典型细胞分裂。

IF 3 4区工程技术

Microscopy and Microanalysis Pub Date : 2026-03-03 DOI: 10.1093/mam/ozag004

Soha Soliman

{"title":"Chondrocyte Division in the Brachial Cartilage of Silver Carp: Atypical Cell Division.","authors":"Soha Soliman","doi":"10.1093/mam/ozag004","DOIUrl":"https://doi.org/10.1093/mam/ozag004","url":null,"abstract":"Studying chondrocyte division in fish has great biological significance in obtaining knowledge helpful for cartilage regeneration. This study focuses on the staged description of the chondrocytes in brachial cartilage in silver carp. Morphological analysis of different stages of chondrocyte division until yield chondrocyte progeny described and discussed the morphological criteria of dividing chondrocytes in brachial cartilage of carp and compared with the known similar features and their indications. The chondrocyte maintains the nuclear membrane during typical cell division. Nuclear changes of the dividing chondrocyte include chromatin condensation (chromatin condensation stage), nuclear indentation (initial lobulation stage), lobulation (lobulation stage), and lobular separation (separation stage). During division, chromatin underwent incomplete condensation that unable to form defined chromosomes. Dividing chondrocytes remained active secretory cells that developed an extensive membranous system of rough endoplasmic reticulum. Chondrocytes exhibited secretory vesicles and cellular extrusion, which probably participate in matrix formation. Chondrocyte division yields chondrocyte progeny located in hemi-circular lacunae. The nuclear-cytoplasmic ratio and the number of chondrocytes at different stages statistically analyzed. Understanding the molecular pathways that regulate atypical chondrocyte division in aquatic species is important to identify a different strategy for propagating chondrocytes and for delivering a potentially useful approach to enhancing cartilage repair and tissue engineering.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"32 2","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147775962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0