Microscopy and Microanalysis最新文献

Operando Freezing Cryogenic Electron Microscopy of Active Battery Materials. 活性电池材料的操作性冷冻低温电子显微镜。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-10-07 DOI: 10.1093/mam/ozae097

Nikita S Dutta, Gerard Michael Carroll, Nathan R Neale, Sang-Don Han, Mowafak Al-Jassim, Katherine Jungjohann

{"title":"Operando Freezing Cryogenic Electron Microscopy of Active Battery Materials.","authors":"Nikita S Dutta, Gerard Michael Carroll, Nathan R Neale, Sang-Don Han, Mowafak Al-Jassim, Katherine Jungjohann","doi":"10.1093/mam/ozae097","DOIUrl":"https://doi.org/10.1093/mam/ozae097","url":null,"abstract":"Understanding structural and chemical evolution of battery materials during operation is critical to achieving safe, efficient, and long-lasting energy storage. Cryogenic electron microscopy (cryo-EM) has become a valuable tool in battery characterization, leveraging low temperatures to improve stability of sensitive materials under electron beam irradiation. However, typical cryo-EM sample preparations leave extended time between the electrochemical point of interest and ex situ freezing of samples, during which active structures may relax, degrade, or otherwise evolve. Here, we detail a method for operando freezing cryo-EM to preserve and characterize native electrode and interfacial structures that arise during battery cycling, based on an operando plunge freezer and cold sample removal process. We validate the method on multiple electrode materials and quantify and discuss the freezing rate achieved. Operando freezing cryo-EM can be used to directly visualize transient features that arise at active electrochemical interfaces, to enable deeper understanding of structural evolution and interfacial chemistry in batteries and other electrochemical systems.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Large-Angle Rocking Beam Electron Diffraction of Large Unit Cell Crystals Using Direct Electron Detector. 使用直接电子探测器对大单元晶胞进行大角度摇摆光束电子衍射

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-10-02 DOI: 10.1093/mam/ozae088

Robert Busch, Hsu-Chih Ni, Yu-Tsun Shao, Jian-Min Zuo

引用次数: 0

Obtaining 3D Atomic Reconstructions from Electron Microscopy Images Using a Bayesian Genetic Algorithm: Possibilities, Insights, and Limitations. 使用贝叶斯遗传算法从电子显微镜图像中获取三维原子重构：可能性、启示和局限。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-10-02 DOI: 10.1093/mam/ozae090

Tom Stoops, Annick De Backer, Ivan Lobato, Sandra Van Aert

引用次数: 0

Utilization of Advanced Microscopy Techniques and Energy-dispersive X-ray Spectroscopy to Characterize Three Piper Species Related to Kava. 利用先进的显微镜技术和能量色散 X 射线光谱分析与卡瓦相关的三种胡椒的特性。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-10-01 DOI: 10.1093/mam/ozae095

Sebastian John Adams, Amar G Chittiboyina, Ikhlas A Khan

{"title":"Utilization of Advanced Microscopy Techniques and Energy-dispersive X-ray Spectroscopy to Characterize Three Piper Species Related to Kava.","authors":"Sebastian John Adams, Amar G Chittiboyina, Ikhlas A Khan","doi":"10.1093/mam/ozae095","DOIUrl":"https://doi.org/10.1093/mam/ozae095","url":null,"abstract":"Macro- and microscopic techniques have long been used to describe plant materials and establish plant structural profiles. These techniques are commonly used in botanical authentication to identify the genuine and closely allied species used in botanical research. Advanced microscopic techniques were used in this study to differentiate three different Piper species used as kava or kava-kava. The genuine species is Piper methysticum and the other two species commonly called false-kava or kava-kava, are Piper auritum and Piper excelsum. Macroscopic characteristics, including a black-spotted stem and fibrous root, are characteristic of P. methysticum, whereas the stem of P. auritum is greenish with no spots, and the P. excelsum stem is purple-pink. Microscopic attributes include the characteristic collenchyma of stems and the pattern of arrangement of peripheral and medullary vascular bundles. The starch grains are smaller in P. excelsum than in the other two species. Energy-dispersive X-ray spectroscopy analysis of the crystals indicates the expected calcium, magnesium, and silica, along with lesser amounts of sodium, and potassium. The crystals present in the Piper species vary in shape, size, and elemental composition. Combining macro- and microscopical techniques and resulting characteristics are instrumental in differentiating the three Piper species.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142365823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Imaging and Segmenting Grains and Subgrains Using Backscattered Electron Techniques. 利用背散射电子技术对晶粒和子晶粒进行成像和细分。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-09-27 DOI: 10.1093/mam/ozae092

Thomas J Bennett, Eric M Taleff

引用次数: 0

Characteristics of the Integument of Phyllomedusa burmeisteri (Anura, Phyllomedusidae) and Boana semilineata (Anura, Hylidae) Males Can Contribute to Understanding its Relation to Habitat. Phyllomedusa burmeisteri (Anura, Phyllomedusidae) 和 Boana semilineata (Anura, Hylidae) 雄性器官的特征有助于了解其与生境的关系。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-09-20 DOI: 10.1093/mam/ozae091

Stéphanie Asséf Millen Valente Teixeira, Mariana Moraes de Castro, Camila Moura Novaes, Daiane Cristina Marques Dos Santos, Charlene da Penha Neves, Luiz Otávio Guimarães-Ervilha, Renato Neves Feio, Mariana Machado-Neves

{"title":"Characteristics of the Integument of Phyllomedusa burmeisteri (Anura, Phyllomedusidae) and Boana semilineata (Anura, Hylidae) Males Can Contribute to Understanding its Relation to Habitat.","authors":"Stéphanie Asséf Millen Valente Teixeira, Mariana Moraes de Castro, Camila Moura Novaes, Daiane Cristina Marques Dos Santos, Charlene da Penha Neves, Luiz Otávio Guimarães-Ervilha, Renato Neves Feio, Mariana Machado-Neves","doi":"10.1093/mam/ozae091","DOIUrl":"https://doi.org/10.1093/mam/ozae091","url":null,"abstract":"The integument of anurans plays vital physiological roles, crucial for understanding the species' survival in their environment. Despite its significance, there are few studies describing the cutaneous morphology of anurans from the Brazilian Atlantic Forest. This study aimed to characterize the integument of Phyllomedusa burmeisteri and Boana semilineata in males using microscopic and histochemical approaches. Histological sections were stained with various dyes, and additional fragments underwent electron microscopy and energy-dispersive X-ray spectroscopy. Results showed different projections on the dorsal and ventral regions of males from these species, without the Eberth-Katschenko layer. Differences in the arrangement of chromatophore cells in regions with varying solar incidence were observed in the spongy dermis. Various gland types were identified, aiding taxonomic differentiation and validation of behavioral data. Both species had seromucous and granular glands, while only P. burmeisteri displayed lipid glands. Histochemical analysis revealed higher production of polysaccharides and proteins, contributing to the integument's moisture and protection. Lipid secretions in P. burmeisteri helped waterproof the integument more effectively against desiccation. This study concludes that analyzing anuran integument provides valuable insights into their behavior, with integument composition potentially influenced by habitat choice among different species.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142291231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Micromorphological and Phytochemical Evaluation of Heliotropium rariflorum Stocks From Karak, Pakistan. 来自巴基斯坦卡拉克的 Heliotropium rariflorum 种群的微形态学和植物化学评估。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-09-20 DOI: 10.1093/mam/ozae085

Noor Ul Uza, Ghulam Dastagir

{"title":"Micromorphological and Phytochemical Evaluation of Heliotropium rariflorum Stocks From Karak, Pakistan.","authors":"Noor Ul Uza, Ghulam Dastagir","doi":"10.1093/mam/ozae085","DOIUrl":"https://doi.org/10.1093/mam/ozae085","url":null,"abstract":"Micromorphological and phytochemical studies play a major role in quality control and standardization of traditional or herbal medications. In the present research, micromorphological assessment of Heliotropium rarifloum stocks was performed through light and scanning electron microscopies (LM & SEM). The anatomy of leaves, stem and root showed salient histological features. Both surfaces of the leaves had setose glandular trichomes measuring 20-38 × 6-15 µm. The lower epidermis had comparatively a maximum anomocytic stomata (16-35) and stomatal index (12-33). The mature pollen grains were small (74 µm) and spheroidal shaped, with psilate exine (2 μm) sculpturing. Vein termination and vein islet number of the upper epidermis were 5-20 and 5-15, respectively. The palisade ratio of the leaf lamina for the upper and lower epidermis was 2-10 and 2-8. LM and SEM of the powdered samples displayed crystals, phloem fibers, xylem, vessels, sieve tube elements, companion cells, and tracheids. Extractive values determination, fluorescence, and phytochemical analysis were employed for quality control according to the World Health Organization (WHO) guidelines. Phytochemical screening revealed various secondary metabolites. It is suggested that H. rariflorum might be a reliable source of nutrients and secondary metabolites and might be more medically effective. The current findings confirm its standardization and validation.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142291234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How Cryo-EM Revolutionized the Field of Bioenergetics. 低温电子显微镜如何彻底改变生物能学领域。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-09-19 DOI: 10.1093/mam/ozae089

Muratha Sottatipreedawong, Ahad Ali Kazmi, Irene Vercellino

{"title":"How Cryo-EM Revolutionized the Field of Bioenergetics.","authors":"Muratha Sottatipreedawong, Ahad Ali Kazmi, Irene Vercellino","doi":"10.1093/mam/ozae089","DOIUrl":"https://doi.org/10.1093/mam/ozae089","url":null,"abstract":"Ten years ago, the term \"resolution revolution\" was used for the first time to describe how cryogenic electron microscopy (cryo-EM) marked the beginning of a new era in the field of structural biology, enabling the investigation of previously unsolvable protein targets. The success of cryo-EM was recognized with the 2017 Chemistry Nobel Prize and has become a widely used method for the structural characterization of biological macromolecules, quickly catching up to x-ray crystallography. Bioenergetics is the division of biochemistry that studies the mechanisms of energy conversion in living organisms, strongly focused on the molecular machines (enzymes) that carry out these processes in cells. As bioenergetic enzymes can be arranged in complexes characterized by conformational heterogeneity/flexibility, they represent challenging targets for structural investigation by crystallography. Over the last decade, cryo-EM has therefore become a powerful tool to investigate the structure and function of bioenergetic complexes; here, we provide an overview of the main achievements enabled by the technique. We first summarize the features of cryo-EM and compare them to x-ray crystallography, and then, we present the exciting discoveries brought about by cryo-EM, particularly but not exclusively focusing on the oxidative phosphorylation system, which is a crucial energy-converting mechanism in humans.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142291233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three-Dimensional Nanoscale Imaging of SiO2 Nanofiller in Styrene-Butadiene Rubber with High-Resolution and High-Sensitivity Ptychographic X-ray Computed Tomography. 利用高分辨率和高灵敏度 X 射线计算机断层扫描对苯乙烯-丁二烯橡胶中的 SiO2 纳米填料进行三维纳米级成像。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-09-19 DOI: 10.1093/mam/ozae094

Naru Okawa, Nozomu Ishiguro, Shuntaro Takazawa, Hideshi Uematsu, Yuhei Sasaki, Masaki Abe, Kyosuke Ozaki, Yoshiaki Honjo, Haruki Nishino, Yasumasa Joti, Takaki Hatsui, Yukio Takahashi

引用次数: 0

The 4D Camera: An 87 kHz Direct Electron Detector for Scanning/Transmission Electron Microscopy. 4D 相机：用于扫描/透射电子显微镜的 87 kHz 直接电子探测器。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-09-19 DOI: 10.1093/mam/ozae086

Peter Ercius, Ian J Johnson, Philipp Pelz, Benjamin H Savitzky, Lauren Hughes, Hamish G Brown, Steven E Zeltmann, Shang-Lin Hsu, Cassio C S Pedroso, Bruce E Cohen, Ramamoorthy Ramesh, David Paul, John M Joseph, Thorsten Stezelberger, Cory Czarnik, Matthew Lent, Erin Fong, Jim Ciston, Mary C Scott, Colin Ophus, Andrew M Minor, Peter Denes

{"title":"The 4D Camera: An 87 kHz Direct Electron Detector for Scanning/Transmission Electron Microscopy.","authors":"Peter Ercius, Ian J Johnson, Philipp Pelz, Benjamin H Savitzky, Lauren Hughes, Hamish G Brown, Steven E Zeltmann, Shang-Lin Hsu, Cassio C S Pedroso, Bruce E Cohen, Ramamoorthy Ramesh, David Paul, John M Joseph, Thorsten Stezelberger, Cory Czarnik, Matthew Lent, Erin Fong, Jim Ciston, Mary C Scott, Colin Ophus, Andrew M Minor, Peter Denes","doi":"10.1093/mam/ozae086","DOIUrl":"https://doi.org/10.1093/mam/ozae086","url":null,"abstract":"We describe the development, operation, and application of the 4D Camera-a 576 by 576 pixel active pixel sensor for scanning/transmission electron microscopy which operates at 87,000 Hz. The detector generates data at ∼480 Gbit/s which is captured by dedicated receiver computers with a parallelized software infrastructure that has been implemented to process the resulting 10-700 Gigabyte-sized raw datasets. The back illuminated detector provides the ability to detect single electron events at accelerating voltages from 30 to 300 kV. Through electron counting, the resulting sparse data sets are reduced in size by 10--300× compared to the raw data, and open-source sparsity-based processing algorithms offer rapid data analysis. The high frame rate allows for large and complex scanning diffraction experiments to be accomplished with typical scanning transmission electron microscopy scanning parameters.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142291236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0