Microscopy and Microanalysis最新文献

Retraction of: Automated Tools to Advance High-Resolution Imaging in Liquid. 撤回：自动化工具，以推进高分辨率成像的液体。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-01-22 DOI: 10.1093/mam/ozae128

引用次数: 0

Retraction of: Structural Insights of the SARS-CoV-2 Nucleocapsid Protein: Implications for the Inner-workings of Rapid Antigen Tests. 撤回：SARS-CoV-2核衣壳蛋白的结构见解：对快速抗原检测内部工作的影响。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-01-22 DOI: 10.1093/mam/ozae127

引用次数: 0

Estimation of Dislocation Densities With Nondestructive Scanning Electron Microscope Techniques: Application to Gallium Nitride. 利用无损扫描电子显微镜技术估算位错密度：氮化镓的应用

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-01-13 DOI: 10.1093/mam/ozae124

Arka Mandal, Benoît Beausir, Julien Guyon, Vincent Taupin, Antoine Guitton

{"title":"Estimation of Dislocation Densities With Nondestructive Scanning Electron Microscope Techniques: Application to Gallium Nitride.","authors":"Arka Mandal, Benoît Beausir, Julien Guyon, Vincent Taupin, Antoine Guitton","doi":"10.1093/mam/ozae124","DOIUrl":"https://doi.org/10.1093/mam/ozae124","url":null,"abstract":"Characterizing threading dislocations (TDs) in gallium nitride (GaN) semiconductors is crucial for ensuring the reliability of semiconductor devices. The current research addresses this issue by combining two techniques using a scanning electron microscope, namely electron channeling contrast imaging (ECCI) and high-resolution electron backscattered diffraction (HR-EBSD). It is a comparative study of these techniques to underscore how they perform in the evaluation of TD densities in GaN epitaxial layers. Experiments reveal that the dislocation line vectors mostly deviate from the growth direction of the film, i.e., ∦ [0001], followed by edge-type dislocations (dislocation lines || [0001]) with insignificant screw character. Furthermore, TDs from the dislocation clusters are characterized as edge- and (edge + mixed)-type TDs. By combining ECCI counting of dislocations and HR-EBSD description of geometrically necessary dislocation density type, it is possible to measure the total TD density and provide the proportion of pure (edge and screw) and mixed TDs. It has also been observed from the analyses of residual elastic strain fields and lattice rotations that it is not possible to identify individual dislocations for the spatial resolution of 50 nm in HR-EBSD. Nevertheless, ECCI and HR-EBSD can be complementarily used to count and characterize the TDs.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142979172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retrieval of Phase Information from Low-Dose Electron Microscopy Experiments: Are We at the Limit Yet? 从低剂量电子显微镜实验中获取相位信息：我们已经到了极限了吗？

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-01-13 DOI: 10.1093/mam/ozae125

Francisco Vega Ibáñez, Jo Verbeeck

{"title":"Retrieval of Phase Information from Low-Dose Electron Microscopy Experiments: Are We at the Limit Yet?","authors":"Francisco Vega Ibáñez, Jo Verbeeck","doi":"10.1093/mam/ozae125","DOIUrl":"https://doi.org/10.1093/mam/ozae125","url":null,"abstract":"The challenge of imaging low-density objects in an electron microscope without causing beam damage is significant in modern transmission electron microscopy. This is especially true for life science imaging, where the sample, rather than the instrument, still determines the resolution limit. Here, we explore whether we have to accept this or can progress further in this area. To do this, we use numerical simulations to see how much information we can obtain from a weak phase object at different electron doses. Starting from a model with four phase values, we compare Zernike phase contrast with measuring diffracted intensity under multiple random phase illuminations to solve the inverse problem. Our simulations have shown that diffraction-based methods perform better than the Zernike method, as we have found and addressed a normalization issue that, in some other studies, led to an overly optimistic representation of the Zernike setup. We further validated this using more realistic 2D objects and found that random phase illuminated diffraction can be up to five times more efficient than an ideal Zernike implementation. These findings suggest that diffraction-based methods could be a promising approach for imaging beam-sensitive materials and that current low-dose imaging methods are not yet at the quantum limit.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142979179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and Anatomical Deformities of Hirudo verbana, Carena 1820 (Annelida, Hirudinea) After Feeding: Light and Electron Microscopic Analysis. 水蛭1820（环节动物，水蛭目）摄食后的形态和解剖畸形：光镜和电镜分析。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-01-13 DOI: 10.1093/mam/ozae136

Özmen Ülger, Huseyin Ayhan

{"title":"Morphological and Anatomical Deformities of Hirudo verbana, Carena 1820 (Annelida, Hirudinea) After Feeding: Light and Electron Microscopic Analysis.","authors":"Özmen Ülger, Huseyin Ayhan","doi":"10.1093/mam/ozae136","DOIUrl":"https://doi.org/10.1093/mam/ozae136","url":null,"abstract":"Leeches are widely used as model organisms in scientific studies and medical treatments. Medical leeches are hematophagous parasites that usually feed on the blood of their hosts. Some leeches show deformities, usually after feeding. This causes both medical and economic losses as it reduces the effectiveness of leeches in cultivation and medical treatment. The aim of this study was to investigate the effects of morphological deformations after feeding in Hirudo verbana, Carena 1820 (Annelida, Hirudinea), an important species in medical leech treatments. For this purpose, both histopathological and scanning electron microscopy examinations of starved and fed leeches were performed. The causes of deformities in medical leeches were found to be due to overfeeding, which caused cracks on the intestinal surface and therefore deterioration of the tissues. It is also thought that the immunological agents in the fed blood destroyed the medical leech tissue in these regions. In addition, it was determined that the cellular structure of the shaped blood elements stored in the cavity after feeding was preserved for a long time (months) without deterioration. It is certain that revealing the mechanism by which this occurs will inspire the preservation of blood for a long time in blood transfusion.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142979176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TBX3 is Essential for Zygotic Genome Activation and Embryonic Development in Pigs. TBX3对猪受精卵基因组激活和胚胎发育至关重要。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-01-13 DOI: 10.1093/mam/ozae123

Xiao-Han Li, Song-Hee Lee, Ji-Dam Kim, Gyu-Hyun Lee, Jae-Min Sim, Xiang-Shun Cui

{"title":"TBX3 is Essential for Zygotic Genome Activation and Embryonic Development in Pigs.","authors":"Xiao-Han Li, Song-Hee Lee, Ji-Dam Kim, Gyu-Hyun Lee, Jae-Min Sim, Xiang-Shun Cui","doi":"10.1093/mam/ozae123","DOIUrl":"https://doi.org/10.1093/mam/ozae123","url":null,"abstract":"The pluripotency-related T-box family transcription factor TBX3 maintains mESC self-renewal and plays a key role in the development of several tissues, including the heart, mammary glands, limbs, and lungs. However, the role of TBX3 during porcine preimplantation embryo development remains unclear. In our research, TBX3 was knocked down by injecting dsRNA to explore the function of TBX3. TBX3 expression gradually increases during early embryonic development. TBX3 knockdown resulted in decreased in the rate of four-cell and blastocyst. Depletion of TBX3 decreased the level of H3K9Ac/H3K27Ac and decreased ZGA gene expression at the four-cell stage. Furthermore, TBX3 knockdown led to a decrease in ZSACN4 protein level, DNMT1 and intracellular 5mc levels were increased, and then induced telomeres shorten and DNA damaged. Additionally, TBX3 knockdown significantly decreased histone acetylation and pluripotency genes NANOG/OCT4 expression in blastocysts. TBX3 knockdown induced apoptosis in blastocysts. Taken together, TBX3 regulate histone acetylation and play important roles in zygotic genome activation and early embryonic development in pigs.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142979180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Self-Opening Transfer Shuttle for the Transfer of Air-Sensitive Sample to Scanning Electron Microscopy. 一种用于空气敏感样品转移到扫描电子显微镜的新型自开转移梭。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-01-13 DOI: 10.1093/mam/ozae120

Peng Wan, Xuri Wang, Qiang Zhang, Zhou Xu, Rui Cai, Yumeng Zhou

引用次数: 0

Heat Transfer Analysis of Cryogenic EXLO Specimen Handling. 低温EXLO样品处理的传热分析。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-01-13 DOI: 10.1093/mam/ozae121

Kyle W Beggs, Thomas E Dougherty, Alain J Kassab, Lucille A Giannuzzi

引用次数: 0

Direct Evidence of Anomalous Peierls Transition-Induced Charge Density Wave Order at Room Temperature in Metallic NaRu2O4. 金属NaRu2O4在室温下异常peerls跃迁诱导电荷密度波序的直接证据。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-01-13 DOI: 10.1093/mam/ozae129

Anna Scheid, Isha, Arvind Kumar Yogi, Masahiko Isobe, Birgit Bußmann, Tobias Heil, Peter A van Aken

{"title":"Direct Evidence of Anomalous Peierls Transition-Induced Charge Density Wave Order at Room Temperature in Metallic NaRu2O4.","authors":"Anna Scheid, Isha, Arvind Kumar Yogi, Masahiko Isobe, Birgit Bußmann, Tobias Heil, Peter A van Aken","doi":"10.1093/mam/ozae129","DOIUrl":"https://doi.org/10.1093/mam/ozae129","url":null,"abstract":"In the field of quantum materials, understanding anomalous behavior under charge degrees of freedom through bond formation is of fundamental importance, with two key concepts: Dimerization and charge order at different cation sites. The coexistence of both dimerization and charge ordering is unusually found in NaRu2O4, even in its metallic state at room temperature. Our work unveils the origin of the interplay of these effects within metallic single-crystalline NaRu2O4. Employing advanced transmission electron microscopy techniques, we probe the lattice order of NaRu2O4 as a function of temperature and provide direct microscopic evidence of a Peierls-type transition. This transition is accompanied by a pronounced dimerization of the ruthenium chains, resulting in a distinctive twofold superstructure along the b axis below the critical transition temperature of ∼535 K, coinciding with a charge order. In situ heating experiments confirm the reversibility of this first-order phase transition, and periodic lattice displacement maps depict atomic-scale displacements linked to dimerization.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142979159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of Small Extracellular Vesicles Using Sequential Ultrafiltration with Regenerated Cellulose Membranes of Different Molecular Weight Cutoffs: A Study of Morphology and Size by Electron Microscopy. 用不同分子量截留物的再生纤维素膜序贯超滤制备细胞外小泡：形貌和大小的电镜研究。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-01-13 DOI: 10.1093/mam/ozae133

Noriyuki Ishii, Hiroaki Tateno

引用次数: 0