Microscopy and Microanalysis最新文献_第2页

On the Formation of Quaternary Particles, Based on Lead-Barium-Antimony + Tin, Considered Characteristic of GSRs. 基于铅-钡-锑+锡的四元粒子的形成，认为是gsr的特征。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-05-09 DOI: 10.1093/mam/ozaf028

Felice Nunziata, Claudio Gentile, Marco Romeo, Giancarlo Palumbo, Gabriele Rotter

{"title":"On the Formation of Quaternary Particles, Based on Lead-Barium-Antimony + Tin, Considered Characteristic of GSRs.","authors":"Felice Nunziata, Claudio Gentile, Marco Romeo, Giancarlo Palumbo, Gabriele Rotter","doi":"10.1093/mam/ozaf028","DOIUrl":"https://doi.org/10.1093/mam/ozaf028","url":null,"abstract":"The presence of tin in gunshot residue (GSR) particles from ammunitions used in short and long rifled firearms is well known and widely documented. Tin does not originate from the primer mixture itself but is typically linked to a thin disc-shaped foil placed over mercury fulminate-based primers to prevent embrittlement of the cartridge case and primer cap. However, this practice has not been previously reported or observed in hunting ammunitions. We designed an experimental protocol to investigate the formation of quaternary GSR particles containing Pb-Ba-Sb-Sn, following shots fired from a double-barrel shotgun using modified hunting ammunitions. These ammunitions were loaded with multiple pellets and included a thin tin foil over the primer's flash hole. Our results confirm that the tin foil is directly responsible for the origin and composition of the quaternary GSR particles detected on the collected stubs. Additionally, the hunting ammunitions market has recently seen increased production of environmentally friendly ammunitions, including shot pellets made of tin, tin alloys, or coated with tin. Our findings provide a foundation for further experimental studies into GSR from such eco-friendly ammunitions, especially in forensic investigations involving tin-containing pellets.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144010124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Histological Analysis of the Parotid and Submandibular Glands in Ovine and Caprine Species Using Histochemical, Immunohistochemical, and Stereological Methods. 用组织化学、免疫组织化学和体视学方法对绵羊和山羊腮腺和下颌骨腺的比较组织学分析。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-05-09 DOI: 10.1093/mam/ozaf025

Diaa Massoud, Mervat A AbdRabou, Attalla F El-Kott, Kareem Morsy, Ali S AlSheri, Sally Negm, Mohamed Abumandour

{"title":"Comparative Histological Analysis of the Parotid and Submandibular Glands in Ovine and Caprine Species Using Histochemical, Immunohistochemical, and Stereological Methods.","authors":"Diaa Massoud, Mervat A AbdRabou, Attalla F El-Kott, Kareem Morsy, Ali S AlSheri, Sally Negm, Mohamed Abumandour","doi":"10.1093/mam/ozaf025","DOIUrl":"https://doi.org/10.1093/mam/ozaf025","url":null,"abstract":"This work was designed to compare the histochemical, immunohistochemical, and stereological features of the parotid and mandibular glands of sheep and goat as representative for ovine and caprine species. The parotid gland was serous in both species, while the mandibular gland was a mixed one. Mucous acini were Periodic Acid-Schiff positive and Aldehyde Fuchsin (AF) negative. Serous acini were AF positive. The parotid gland of goat was significantly larger than that of sheep; however, the mandibular gland of sheep was significantly larger than that of goat. The volume of isolated serous acini in the mandibular gland of goat was significantly higher than that of sheep. The total length of ductal system was nonsignificant in both species, but the total volume of parotid glands' ducts was significantly higher than those of mandibular glands. The parotid glands were negative for Mucin-1; however, the mandibular glands were moderately positive. Cytokeratin 7 exhibited a pronounced expression within the ductal systems of both glands. S100 proteins were expressed intensely by serous acini of glands in sheep; however, its expression was confined to the intercalated ducts and myoepithelial cells in goat. The obtained results suggest that the feeding modalities may lead to significant variations in histological and histophysiological characteristics.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-03-17 DOI: 10.1093/mam/ozaf021

Diaa Massoud, Mervat A AbdRabou, Khadija Abdul Jalil Faddladdeen, Attalla F El-Kott, Mohamed M A Abumandour, Eman Kamal Khalil

{"title":"Age-related Histological and Histochemical Changes of the Uropygial Gland in the Domestic Pigeon (Columba livia).","authors":"Diaa Massoud, Mervat A AbdRabou, Khadija Abdul Jalil Faddladdeen, Attalla F El-Kott, Mohamed M A Abumandour, Eman Kamal Khalil","doi":"10.1093/mam/ozaf021","DOIUrl":"https://doi.org/10.1093/mam/ozaf021","url":null,"abstract":"The present investigation aimed to analyze the uropygial gland's (UG) histological architecture and histochemical variation in the domestic pigeon at four posthatching age stages. The small and relatively undeveloped UG was observed at 1-day-old, and then the gland started to increase in size and complexity with age to reach its full functional size in the adult. The stroma became more organized with increased collagen fiber deposition, supporting the growing gland with age. The secretory tubules were more developed in the mature and adult pigeons than in other ages. The epithelial layer had four layers: basal layer, intermediate layer, secretory layer, and degenerative layer. The histochemical analysis using Mallory's trichrome stain revealed a positive reaction with all ages. The capsule, interlobular septum, papilla areas, and CT appeared blue, and the cellular components appeared red. The staining intensity increases with age till it becomes intensely blue with well-developed collagen in adults. Herbst corpuscles were encapsulated by a connective tissue sheath and comprised nerve cells arranged along the central axon, enveloped by collagen fibers. The findings from the current study may have a potential implication in poultry science and developmental biology.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144031602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Post-hatch Development of the Adrenal Gland in Chickens (Gallus gallus domesticus): Insights From Histology, Immunohistochemistry, Transmission Electron Microscopy, and Catecholamine Level Analysis. 鸡（Gallus Gallus domesticus）肾上腺的孵化后发育：来自组织学、免疫组织化学、透射电镜和儿茶酚胺水平分析的见解。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-03-17 DOI: 10.1093/mam/ozaf018

Eman Kamal Khalil, Eman M El-Beltagi, Mohamed M A Abumandour, Maha A Aljumaa, Eman Fayad, Aya H Tantawy

{"title":"Post-hatch Development of the Adrenal Gland in Chickens (Gallus gallus domesticus): Insights From Histology, Immunohistochemistry, Transmission Electron Microscopy, and Catecholamine Level Analysis.","authors":"Eman Kamal Khalil, Eman M El-Beltagi, Mohamed M A Abumandour, Maha A Aljumaa, Eman Fayad, Aya H Tantawy","doi":"10.1093/mam/ozaf018","DOIUrl":"10.1093/mam/ozaf018","url":null,"abstract":"This study is the first to describe posthatching morphological changes in chicken adrenal glands using histology, immunohistochemistry, transmission electron microscopy, and catecholamine levels. The study involved 30 chickens in 3 posthatching stages: Juvenile (1 day old), immature (2 months old), and mature (6 months old). The results showed no demarcation between cortical and medullary, with the interrenal tissue comprising most of the adrenal parenchyma. The 1-day-old chickens did not exhibit the zoning of interrenal tissue into subcapsular and inner zones, while as they aged, the proportion of medullary tissue increased. The norepinephrine-secreting cells exhibited a significantly higher number of secretory granules compared to the epinephrine-secreting cells across the different stages. The plasma levels of catecholamines increased with advancing age. Chromogranin immunoactivity increased with age during the development of the adrenal gland. It can be concluded that the adrenal gland undergoes significant structural and functional changes during various posthatching developmental periods. In general, the study provides valuable insights into the growth and maturation of the adrenal glands in posthatch chickens, potentially aiding in understanding their maturation and their role in controlling catecholamines. In conclusion, the findings suggest that optimizing stress responses in chickens could enhance poultry production, potentially leading to the development of new stress management strategies.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantifying Patterned Features on Material Surfaces Created using Ga Ion Beam in FIB-SEM. 在FIB-SEM中使用Ga离子束创建材料表面的定量化图案特征。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-03-17 DOI: 10.1093/mam/ozaf001

Supriya Ghosh, K Andre Mkhoyan

{"title":"Quantifying Patterned Features on Material Surfaces Created using Ga Ion Beam in FIB-SEM.","authors":"Supriya Ghosh, K Andre Mkhoyan","doi":"10.1093/mam/ozaf001","DOIUrl":"https://doi.org/10.1093/mam/ozaf001","url":null,"abstract":"We introduced and applied a set of parameters to quantify surface modifications and pattern resolutions made by a Ga ion beam in a focused ion beam instrument using two material systems, Si and SrTiO3. A combination of top-view scanning electron microscopy and cross-sectional scanning transmission electron microscopy imaging and energy-dispersive X-ray spectroscopy was used to study the structure, composition and measure dimensions of the patterned lines. The total ion dose was identified as the key parameter governing the line characteristics, which can be controlled by the degree of overlap among adjacent spots, beam dwell time at each spot, and number of beam passes for given beam size and current. At higher ion doses (>1015 ions/cm2), the Ga ions remove part of the material in the exposed area creating \"channels\" surrounded with amorphized regions, whereas at lower ion doses only amorphization occurs, creating \"ridges\" on the wafer surface. To pattern lines with similar sizes, an order of magnitude different ion doses was required for Si and SrTiO3 indicating strong material dependence. Quantification revealed that lines as fine as 10 nm can be reproducibly patterned and characterized on the surfaces of materials.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Dynamic Reconstruction on Particle Size and Morphology in Atom Probe Tomography. 动态重建对原子探针层析成像中颗粒大小和形貌的影响。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-03-17 DOI: 10.1093/mam/ozaf006

Constantinos Hatzoglou, Paraskevas Kontis, Heena Khanchandani, Gerald Da Costa, Baptiste Gault, François Vurpillot

引用次数: 0

The Role of the CXCL12/CXCR4 Signaling Pathway in Regulating Cellular Migration. CXCL12/CXCR4信号通路在调控细胞迁移中的作用

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-03-17 DOI: 10.1093/mam/ozaf011

Austin N Worden, Emma Grace Pittard, Matt Stern, Mark J Uline, Jay D Potts

{"title":"The Role of the CXCL12/CXCR4 Signaling Pathway in Regulating Cellular Migration.","authors":"Austin N Worden, Emma Grace Pittard, Matt Stern, Mark J Uline, Jay D Potts","doi":"10.1093/mam/ozaf011","DOIUrl":"10.1093/mam/ozaf011","url":null,"abstract":"We investigated the CXCL12/CXCR4 signaling pathway as a regulator of adipose-derived stem cell (ADSC) self-assembling toroidal constructs using collagen hydrogels. ADSCs formed toroid rings when cultured on hydrogel surfaces but failed to do so when mixed within the matrix. Gene expression profiling revealed significant upregulation of the CXCL12/CXCR4 pathway in toroid-forming conditions, supported by immunofluorescence studies that confirmed CXCL12 presence in toroids but not in mixed-in cultures. Early toroid formation was marked by the emergence of CXCL12 expression, correlating with cell migration. Targeted inhibition experiments identified the PI3K pathway as a critical regulator, delaying cell migration by ∼16 h, while N-Cadherin, Ras/Raf, and ERK1/2 inhibition either reduced or halted migration over extended periods. Through Western blot analysis, altered expression of α-Smooth muscle actin and focal adhesion kinase under PI3K inhibition was highlighted thus emphasizing their roles in toroid formation. Lastly, initial coculture studies with 4T1 breast cancer cells unexpectedly showed CXCL12 localization primarily in 4T1 cells within mixed toroids, suggesting modified chemotactic signaling. Our findings establish CXCL12/CXCR4 as crucial for ADSC toroid formation and reveal the pathway's complex involvement in cellular organization and migration, presenting a robust model for exploring cell-cell and cell-matrix interactions relevant to tissue engineering and cancer research.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Efficient Method for Preparing High-Quality Atom Probe Tomography Samples of Dual-Phase Ti Alloys. 制备高质量双相钛合金原子探针断层扫描样品的高效方法

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-03-17 DOI: 10.1093/mam/ozaf009

Ruiqing Lu, Felix Theska, Shenglu Lu, Hao Wang, Ma Qian, Sophie Primig, Simon P Ringer, Xiaozhou Liao

引用次数: 0

Mixed Element Microparticle Characterization by Electron Probe Microanalysis. 混合元素微粒的电子探针分析表征。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-03-17 DOI: 10.1093/mam/ozaf022

Alexis T Riche, Peter McSwiggen, Kayleigh Harvey, Shelby Bowden, Rachel A Bergin, Spencer M Scott, Bryan J Foley, Kyle M Samperton

{"title":"Mixed Element Microparticle Characterization by Electron Probe Microanalysis.","authors":"Alexis T Riche, Peter McSwiggen, Kayleigh Harvey, Shelby Bowden, Rachel A Bergin, Spencer M Scott, Bryan J Foley, Kyle M Samperton","doi":"10.1093/mam/ozaf022","DOIUrl":"https://doi.org/10.1093/mam/ozaf022","url":null,"abstract":"Microparticle compositional characterization for nuclear forensics has traditionally been achieved by \"gold-standard\" destructive analytical techniques such as large geometry-secondary ion mass spectrometry and fission track-thermal ionization mass spectrometry, but long processing times and total sample consumption eliminate the option of subsequent analysis by other methods. Electron probe microanalysis has long been widely employed for rapid, nondestructive, micrometer-scale compositional measurements and imaging in fields such as material science and geology and may therefore need to be reevaluated as an alternative tool for microparticle analysis for the nuclear forensics community. This study presents the use of electron probe microanalysis for imaging and quantitative characterization of homogeneous microparticles utilizing a calibration curve based on high-precision quadrupole-inductively coupled plasma-mass spectrometry analyses. Samples of opportunity synthesized at Savannah River National Laboratory, nickel-doped cerium oxide microparticles, were selected as analogs for plutonium-doped uranium oxide microparticles. Positive detection and accurate quantification of variable amounts of nickel dopant down to trace levels (10s of parts per million) suggest applicability of this technique to other mixed element systems (e.g., actinides). Quantitative single-particle characterization via electron probe microanalyzer may thus provide a high fidelity, nondestructive complement to techniques currently in use.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developmental Dynamics of the Rabbit Cerebellum During Fetal Maturation With Insights into the Role of Radial Glia in Neuronal Development. 兔胎儿成熟过程中小脑的发育动态及径向胶质细胞在神经元发育中的作用。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-03-17 DOI: 10.1093/mam/ozaf015

Manal T Hussein, Norhan M Kotb, Doaa M Mokhtar, Marwa M Hussein

{"title":"Developmental Dynamics of the Rabbit Cerebellum During Fetal Maturation With Insights into the Role of Radial Glia in Neuronal Development.","authors":"Manal T Hussein, Norhan M Kotb, Doaa M Mokhtar, Marwa M Hussein","doi":"10.1093/mam/ozaf015","DOIUrl":"10.1093/mam/ozaf015","url":null,"abstract":"This study examines the development of the rabbit cerebellum from the 10th day postconception to full-term fetal age, with a particular focus on the role of radial glial cells in the differentiation of cerebellar neurons. A total of 35 embryonic samples were meticulously dissected and microscopically analyzed. On embryonic day (ED) 12, cerebellar primordia, consisting of the ventricular neuroepithelium and rhombic lip, were observed. By ED16, significant neuronal cell proliferation and migration in both the radial and tangential directions were noted. On ED 20, lamination processes began, forming the external granular layer (EGL) and Purkinje cell plate (PCP) with the support of radial glial cells. By ED 25, the cerebellar cortex had developed three distinct layers: the EGL, PCP, and the prospective molecular layer (PML), with radial glial cells localized in the PCP. Differentiation continued, and upon ED30, a new cortical layer, the internal granular layer, was evident. Additionally, the gradual replacement of nestin by glial fibrillary acidic protein marked the differentiation of radial glia into Bergmann glia at ED 25 and ED 30. β-III tubulin, a marker of differentiated neurons, was detected in the inner layer of EGL and PCP during these stages. In conclusion, this study highlights the pivotal role of radial glial cells in the layered organization and neuronal differentiation of the developing rabbit cerebellum. The developmental trajectory observed provides valuable insights into cerebellar morphogenesis and supports the relevance of the rabbit model for exploring neurodevelopmental processes.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0