Microscopy and Microanalysis最新文献_第3页

Startling C Behavior in Mo-Free Steel Atom Probe Tomography Volumes. 无钼钢原子探针层析成像卷中惊人的C行为。

IF 3 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-07-15 DOI: 10.1093/mam/ozaf078

William Mottay, Jeremy Landes, Alain Portavoce, Benjamin Klaes, Frederic Danoix, Philippe Maugis, Pierre Stocker, Carine Perrin-Pellegrino, Khalid Hoummada

{"title":"Startling C Behavior in Mo-Free Steel Atom Probe Tomography Volumes.","authors":"William Mottay, Jeremy Landes, Alain Portavoce, Benjamin Klaes, Frederic Danoix, Philippe Maugis, Pierre Stocker, Carine Perrin-Pellegrino, Khalid Hoummada","doi":"10.1093/mam/ozaf078","DOIUrl":"https://doi.org/10.1093/mam/ozaf078","url":null,"abstract":"Atom probe tomography (APT) is now routinely used to study solute atom segregation at crystalline defects in different materials. The present study reports unexpected observations concerning carbon (C) segregation at dislocations in APT volumes analyzed from two different industrial steel grades. APT analyses reveal that C segregation at dislocations could only be observed with Mo co-segregation. Indeed, transmission electron microscopy (TEM) observations on APT tips and correlative TEM-APT analysis show that despite dislocations being present in the samples prior to APT analyses, C segregation was not observed in the absence of Mo segregation. Statistics on the distribution of C composition in the different APT volumes from Mo-free steels show important discrepancies, with 35% of the volumes exhibiting C content in solid solution five times higher than expected. It is concluded that APT measurements of both C segregation at dislocations and C content in solution in iron may be incorrect due to the possibility of dislocations leaving the APT samples when subjected to a high electric field before or during field evaporation.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 4","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Practical Considerations for Crystallographic and Microstructure Mapping With Direct Electron Detector-Based Electron Backscatter Diffraction. 基于直接电子探测器的电子背散射衍射的晶体学和微观结构映射的实际考虑。

IF 3 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-07-15 DOI: 10.1093/mam/ozaf076

Tianbi Zhang, Ruth M Birch, Graeme J Francolini, Ebru Karakurt Uluscu, Ben Britton

引用次数: 0

PtyRAD: A High-Performance and Flexible Ptychographic Reconstruction Framework with Automatic Differentiation. PtyRAD：一种高性能、灵活的带自动分化的ptygraphic重建框架。

IF 3 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-07-15 DOI: 10.1093/mam/ozaf070

Chia-Hao Lee, Steven E Zeltmann, Dasol Yoon, Desheng Ma, David A Muller

{"title":"PtyRAD: A High-Performance and Flexible Ptychographic Reconstruction Framework with Automatic Differentiation.","authors":"Chia-Hao Lee, Steven E Zeltmann, Dasol Yoon, Desheng Ma, David A Muller","doi":"10.1093/mam/ozaf070","DOIUrl":"10.1093/mam/ozaf070","url":null,"abstract":"Electron ptychography has recently achieved unprecedented resolution, offering valuable insights across diverse material systems, including in three dimensions. However, high-quality ptychographic reconstruction is computationally expensive and time consuming, requiring a significant amount of manually tuning even for experts. Additionally, essential tools for ptychographic analysis are often scattered across multiple software packages, with some advanced features available only in costly commercial software like MATLAB. To address these challenges, we introduce PtyRAD (Ptychographic Reconstruction with Automatic Differentiation), an open-source software framework offers a comprehensive, flexible, and computationally efficient solution for electron ptychography. PtyRAD provides seamless optimization of multiple parameters-such as sample thickness, local tilts, probe positions, and mixed probe and object modes-using gradient-based methods with automatic differentiation. By utilizing PyTorch's highly optimized tensor operations, PtyRAD achieves up to a 24× speedup in reconstruction time compared to existing packages without compromising image quality. In addition, we propose a real-space depth regularization, which avoids wrap-around artifacts and can be useful for twisted two-dimensional material datasets and vertical heterostructures. Moreover, PtyRAD integrates a Bayesian optimization workflow that streamlines hyperparameter selection. We hope the open-source nature of PtyRAD will foster reproducibility and community-driven development for future advances in ptychographic imaging.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 4","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144883196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RAPID-DM: A DigitalMicrograph® Script for On-Site Indexing of Zone Axis Aligned Electron Diffraction Patterns of Cubic Lattices. RAPID-DM：一个数字显微图像®脚本，用于现场索引的区域轴对齐电子衍射模式的立方晶格。

IF 3 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-07-15 DOI: 10.1093/mam/ozaf060

Vasilis A Maroufidis, Thomas E Weirich

{"title":"RAPID-DM: A DigitalMicrograph® Script for On-Site Indexing of Zone Axis Aligned Electron Diffraction Patterns of Cubic Lattices.","authors":"Vasilis A Maroufidis, Thomas E Weirich","doi":"10.1093/mam/ozaf060","DOIUrl":"10.1093/mam/ozaf060","url":null,"abstract":"A DigitalMicrograph® script RAPID-DM (RAtio method Pattern InDexing) has been developed, which allows instant on-site indexing of zone axis electron diffraction patterns of cubic lattices using the Rn ratio principle. In addition to indexing spot electron diffraction patterns, the program is also capable of indexing Kikuchi patterns taken from or near a zone axis. Both cases are demonstrated by examples for silicon. The program has a guided workflow and requires only three user-defined lines or Kikuchi bands for indexing. RAPID-DM has been extensively tested and verified to work reliably with both calibrated and noncalibrated zone axis patterns and allows the user to easily evaluate whether the material under examination is cubic, pseudo-cubic, or neither. For calibrated patterns, the program provides an average value of the cubic lattice parameter, which can serve for phase identification in connection with a structural database or it can simply be used to verify the material under investigation. In its current state, the developed script has proved to be a valuable add-on to the DigitalMicrograph® platform in the authors' service laboratory, as it greatly simplifies on-site crystallographic analysis of electron diffraction patterns of steels, alloys, and ceramics, which frequently form cubic or pseudo-cubic structures.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 4","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144883197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tiny Bubbles: Combined HR(S)TEM and 4D-STEM Analysis of Sub-Nanometer He Bubbles in Au. 微小气泡：金中亚纳米He气泡的HR(S)TEM和4D-STEM联合分析

IF 3 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-07-15 DOI: 10.1093/mam/ozaf072

Sean H Mills, Christoph Gammer, Alex Lin, Aaron Kohnert, Claire Chisholm, Khalid Hattar, Peter Ercius, Peter Hosemann, Andrew M Minor

引用次数: 0

Influence of Beam Size, Landing Energy, and Charging Effects on the Apparent Diameter of Substrate-Supported Single-Walled Carbon Nanotubes in Scanning Electron Microscopy Imaging. 扫描电子显微镜成像中光束尺寸、着陆能量和充电效应对单壁碳纳米管表观直径的影响

IF 3 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-07-15 DOI: 10.1093/mam/ozaf065

Boxiang Zhang, Xiaoxiao Guan, Chenjian Yang, Renzhao Xu, Yuanfang Zhang, Qingyun Lin, Xiaoming Ma, Chuanhong Jin

{"title":"Influence of Beam Size, Landing Energy, and Charging Effects on the Apparent Diameter of Substrate-Supported Single-Walled Carbon Nanotubes in Scanning Electron Microscopy Imaging.","authors":"Boxiang Zhang, Xiaoxiao Guan, Chenjian Yang, Renzhao Xu, Yuanfang Zhang, Qingyun Lin, Xiaoming Ma, Chuanhong Jin","doi":"10.1093/mam/ozaf065","DOIUrl":"10.1093/mam/ozaf065","url":null,"abstract":"Due to the apparent width/diameter broadening common for sub-10 nm features, scanning electron microscopy faces many challenges for nanometrology in silicon-based and emerging carbon nanotube (CNT)-based technologies. The influence of beam size (σbeam), landing energy (LE), and charging on the apparent diameter of CNTs (WCNT) is investigated here. Experiments show WCNT increases with increasing σbeam, decreases with increasing LE, and shows little variation between conductive Si and insulating SiO2/Si substrates. Monte Carlo simulations show WCNT remains unchanged with σbeam smaller than ∼1/6 of CNT diameters (dCNT) but begins to increase once σbeam becomes larger, and WCNT varies little with increasing LE if σbeam is fixed. These results suggest (1) σbeam is decisive in the WCNT broadening; (2) the effect of LE is attributed to the change in σbeam instead of the width of interaction volume; and (3) the contribution of charging is minimal with the contrast separation method. We also notice that increasing the LE beyond 3 keV makes CNT almost invisible. This is attributed to the too-small ratio of electron-CNT interaction volume to the electron-substrate interaction volume. Testing LEs ranging from 0.3 to 10 keV, we find optimal balancing of WCNT and visibility in the 0.5-1.0 keV range.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 4","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144883191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strategies for Preparing and Analyzing Thin Passive Films With Atom Probe Tomography. 用原子探针层析技术制备和分析钝化薄膜的策略。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-07-15 DOI: 10.1093/mam/ozaf023

Elizabeth J Kautz, Kayla H Yano, Josephine C Hartmann, Angela Y Gerard, Sandra D Taylor, John R Scully, Daniel K Schreiber

{"title":"Strategies for Preparing and Analyzing Thin Passive Films With Atom Probe Tomography.","authors":"Elizabeth J Kautz, Kayla H Yano, Josephine C Hartmann, Angela Y Gerard, Sandra D Taylor, John R Scully, Daniel K Schreiber","doi":"10.1093/mam/ozaf023","DOIUrl":"https://doi.org/10.1093/mam/ozaf023","url":null,"abstract":"Atom probe tomography (APT) provides a unique, three-dimensional map of elemental and isotopic distributions over a wide range of materials with near-atomic scale resolution and is particularly strong at analyzing buried interfaces within materials. However, it is much more difficult to apply atom probe to the analysis of nanoscale surface films, such as those formed during alloy passivation, where unique challenges persist for sample preparation and data collection. Here, we present sample preparation strategies involving the deposition of a <100 nm capping layer that enables reliable characterization of thin passive films ∼2-5 nm thick formed on binary and multiprincipal element alloys via APT. Several capping layer materials (Pt, Ti, and Ni/Cr bilayer) and deposition methods are contrasted. Our results indicate a sputtered Ni/Cr bilayer enables the characterization of the entire passive film and concentration profiles that can easily be interpreted to clearly distinguish base alloy/passive film/capping layer interfaces. Lastly, we highlight ongoing challenges and opportunities for this experimental approach.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 4","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144699012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ameliorative Potential of Mesenchymal Stem Cells-derived Exosomes on the Cerebellar Cortex in a Rat Model of Parkinson's Disease: Targeting HOTAIR/miRNA-221 Signaling Axis: A Histological, Immunohistochemical, and Biochemical Study. 针对HOTAIR/miRNA-221信号轴的间质干细胞衍生外泌体在帕金森病大鼠模型小脑皮层的改善潜力：组织学、免疫组织化学和生化研究

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-07-15 DOI: 10.1093/mam/ozaf062

Amany Mohamed Shalaby, Nema Soliman, Amira Mostafa Elshamy, Sulaiman Mohammed Alnasser, Mohammed Alorini, Hamad Alsaykhan, Fatima A Jaber, Mohamed Ali Alabiad, Amr Mohamed Younes, Mohamed Mahmoud Abdelrahim Elshaer, Walaa E Omar, Hanim Magdy Abdelnour

{"title":"Ameliorative Potential of Mesenchymal Stem Cells-derived Exosomes on the Cerebellar Cortex in a Rat Model of Parkinson's Disease: Targeting HOTAIR/miRNA-221 Signaling Axis: A Histological, Immunohistochemical, and Biochemical Study.","authors":"Amany Mohamed Shalaby, Nema Soliman, Amira Mostafa Elshamy, Sulaiman Mohammed Alnasser, Mohammed Alorini, Hamad Alsaykhan, Fatima A Jaber, Mohamed Ali Alabiad, Amr Mohamed Younes, Mohamed Mahmoud Abdelrahim Elshaer, Walaa E Omar, Hanim Magdy Abdelnour","doi":"10.1093/mam/ozaf062","DOIUrl":"https://doi.org/10.1093/mam/ozaf062","url":null,"abstract":"Among neurodegenerative diseases, Parkinson's disease (PD) is the second most common disorder. It is marked by the degeneration of dopaminergic neurons and depletion of dopamine. The mesenchymal stem cells (MSCs)-derived exosomes hold a promise for addressing neurodegeneration-associated neurological disorders owing to their distinctive immunomodulatory and regenerative properties. The investigation explored the therapeutic potential of MSCs-derived exosomes to mitigate the pathological changes in the cerebellar cortex in a rat model of PD. Thirty rats were divided into control, PD, and PD-BM-MSCs-derived exosomes groups. For 5 weeks, rodents were administered a subcutaneous injection of 2 mg/kg/day of rotenone to induce a PD model. The PD group exhibited a substantial increase in relative cerebellar mRNA HOTAIR, BAX, and caspase 3 gene expression, along with a concomitant decrease in relative cerebellar miRNA-221 gene expression. Light and transmission electron microscopy also depicted marked degenerative changes in the cerebellar cortex. The immune expression of glial fibrillary acidic protein and ionized calcium-binding adaptor molecule-1 markedly increased, while synaptophysin expression markedly decreased. Interestingly, all changes showed a significant regression following treatment with exosomes derived from BM-MSCs. In conclusion, BM-MSCs-derived exosomes may be a promising PD intervention strategy.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 4","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlative Imaging of Structural Biochemistry in Plant and Food Quality Research Within an Interoperable Data Acquisition Platform. 基于互操作数据采集平台的植物结构生物化学相关成像与食品质量研究

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-07-15 DOI: 10.1093/mam/ozaf057

Marjana Regvar, Boštjan Jenčič, Martin Šala, Aleš Kladnik, Iztok Dogša, Maja Koblar, Mitja Kelemen, Primož Vavpetič, Katarina Vogel-Mikuš, Ivan Kreft, Primož Pelicon, Paula Pongrac

{"title":"Correlative Imaging of Structural Biochemistry in Plant and Food Quality Research Within an Interoperable Data Acquisition Platform.","authors":"Marjana Regvar, Boštjan Jenčič, Martin Šala, Aleš Kladnik, Iztok Dogša, Maja Koblar, Mitja Kelemen, Primož Vavpetič, Katarina Vogel-Mikuš, Ivan Kreft, Primož Pelicon, Paula Pongrac","doi":"10.1093/mam/ozaf057","DOIUrl":"https://doi.org/10.1093/mam/ozaf057","url":null,"abstract":"Correlative imaging is a powerful tool for revealing information on cell-type structures and their biochemistry, with the potential to inform healthier food choices and improved dietary recommendations. Determination of plant structures and their structural biochemistry advances our understanding of specific structures designed to store different biomolecules within cells and tissues. Compared to the classical biochemical separation techniques, the key advantage of sequential correlative imaging techniques is in relating spatial plant (micro)structures to their biochemistry in a nondestructive manner. Sequential imaging reported here comprises six methodologies on a single sample, a cross-section of a Tartary buckwheat (Fagopyrum tataricum) grain, namely, bright-field and autofluorescence microscopy, fluorescence microspectroscopy, MeV-secondary ion mass spectrometry, micro-particle-induced X-ray emission, scanning electron microscopy coupled with energy dispersive X-ray spectroscopy, and laser ablation-inductively coupled plasma-mass spectrometry. Results confirm that the stepwise addition of the desired information across several classes of biomolecules and several spatial scales informs the quality and safety of plant-based produce across scales. Therefore, a viable workflow is proposed, enabling sequential spatial analysis of grain and highlighting plant structures' in situ specificity. The advantages and disadvantages of the selected methodologies were critically evaluated.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 4","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mapping the Path to Cryogenic Atom Probe Tomography Analysis of Biomolecules. 绘制生物分子低温原子探针层析分析路径。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-07-15 DOI: 10.1093/mam/ozaf032

Eric V Woods, Tim M Schwarz, Mahander P Singh, Shuo Zhang, Se-Ho Kim, Ayman A El-Zoka, Lothar Gremer, Dieter Willbold, Ingrid McCarroll, Baptiste Gault

{"title":"Mapping the Path to Cryogenic Atom Probe Tomography Analysis of Biomolecules.","authors":"Eric V Woods, Tim M Schwarz, Mahander P Singh, Shuo Zhang, Se-Ho Kim, Ayman A El-Zoka, Lothar Gremer, Dieter Willbold, Ingrid McCarroll, Baptiste Gault","doi":"10.1093/mam/ozaf032","DOIUrl":"https://doi.org/10.1093/mam/ozaf032","url":null,"abstract":"The understanding of protein structure and interactions remains a fundamental challenge in modern biology. While X-ray and electron-based techniques have provided atomic-level protein configurations, they require numerous molecules for averaged views and lack detailed compositional information crucial for biochemical activity. Atom probe tomography (APT) emerges as a promising tool for biological material analysis, though its capabilities for examining biomolecules in their native, hydrated state remain largely unexplored. We present systematic analyses of amino acids in frozen aqueous solutions using two different nanoporous metal supports across various analysis conditions. Our methodology employs a complete cryogenic workflow, including drop-casting, inert gas glovebox freezing, and specimen transfer via a cryogenically cooled ultra-high vacuum shuttle to both focused ion beam microscopy and atom probes. Using water molecular ion ratios as electrostatic field condition indicators, we investigate amino acid fragmentation and behavior. We evaluate the critical factors for successful biomolecular analysis: support material selection, cryogenic specimen preparation, and optimal data acquisition parameters. This work establishes guidelines for cryogenic APT analysis of biomolecules, advancing the technique's application in biological sciences.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 4","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144708132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0