Microscopy and Microanalysis最新文献_第3页

Ultrastructural Study of Ciliary Fragmentation in Renal Tubules During Ischemia-Reperfusion Injury. 缺血再灌注损伤时肾小管纤毛断裂的超微结构研究。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-05-09 DOI: 10.1093/mam/ozaf042

Mungunchimeg Chultemsuren, Su-Youn Lee, Mi Jin Jee, Kwon Moo Park, Ki-Hwan Han

{"title":"Ultrastructural Study of Ciliary Fragmentation in Renal Tubules During Ischemia-Reperfusion Injury.","authors":"Mungunchimeg Chultemsuren, Su-Youn Lee, Mi Jin Jee, Kwon Moo Park, Ki-Hwan Han","doi":"10.1093/mam/ozaf042","DOIUrl":"https://doi.org/10.1093/mam/ozaf042","url":null,"abstract":"Primary cilia on renal tubular cells are critical for kidney function. This study investigated how acute kidney injury affects cilia length, specifically focusing on their shortening during ischemia-reperfusion injury. Using Sprague-Dawley rats, renal pedicles were clamped for 30 min followed by six hours of reperfusion. Kidney tissues were analyzed using confocal laser scanning microscopy and scanning electron microscopy. Acetylated α-tubulin and specific markers such as aquaporin 1 and H + -ATPase were used to identify proximal tubules and collecting ducts. Under normal conditions, renal tubular cells exhibited long primary cilia. Following injury, significant shortening of primary cilia was observed in both proximal tubules and collecting ducts. Intermingled microvilli in proximal tubules complicated ultrastructural observation. However, the collecting duct exhibited distinct responses. While intercalated cells detached into the lumen, principal cells remained attached to the basal lamina. Electron microscopy confirmed the shortening of cilia in principal cells, often accompanied by bulging regions of varying sizes, observed at either the tip or middle, and appearing as single or multiple structures. These findings demonstrate that acute ischaemic injury induces ciliary fragmentation in collecting duct principal cells. The observed bulging in cilia undergoing shortening may indicate potential mechanisms underlying ciliary fragmentation.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histological Insights into the Distribution of Immune Cells in the Esophagus in Koi Fish (Cyprinus rubrofuscus). 锦鲤食道免疫细胞分布的组织学观察。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-05-09 DOI: 10.1093/mam/ozaf046

Maha K Mohamed, Madeha Ahmed Hashim, Enas A Abd-Elhafez, Nada Abdellah

{"title":"Histological Insights into the Distribution of Immune Cells in the Esophagus in Koi Fish (Cyprinus rubrofuscus).","authors":"Maha K Mohamed, Madeha Ahmed Hashim, Enas A Abd-Elhafez, Nada Abdellah","doi":"10.1093/mam/ozaf046","DOIUrl":"https://doi.org/10.1093/mam/ozaf046","url":null,"abstract":"The esophagus of koi fish plays a critical role in digestion and immune defense, as it is directly exposed to a variety of pathogens. This study investigates the histological features and the immune cell composition and enrichment within the esophagus of koi fish, aiming to understand the local immune response mechanisms. Histological and immunohistochemical analyses were conducted on esophagus samples from koi fish, focusing on the distribution of various immune cells. Our results indicate a notable enrichment of immune cells (lymphocytes, macrophages, rodlet cell) in the esophageal mucosa. In addition, the presence of goblet cells, club cells, and telocytes suggests that they play a role in immune cell regulation. The study highlights the adaptive immune system's presence in this region and suggests that the esophagus may serve as a crucial site for mucosal immunity, offering a first line of defense against ingested pathogens through mechanical barriers and immune activation. Understanding the immune cell dynamics in the koi fish esophagus could provide insights into fish immunology and help improve aquaculture health management practices.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Innovative Transmission Electron Microscopy-Based Ultrastructural Imaging Methodology for the Diagnosis of Respiratory Ciliary Disorders. 一种基于透射电子显微镜的诊断呼吸道纤毛疾病的创新超微结构成像方法。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-05-09 DOI: 10.1093/mam/ozaf033

Shikha Chaudhary, Kana Ram Jat, Subhash Chandra Yadav

{"title":"An Innovative Transmission Electron Microscopy-Based Ultrastructural Imaging Methodology for the Diagnosis of Respiratory Ciliary Disorders.","authors":"Shikha Chaudhary, Kana Ram Jat, Subhash Chandra Yadav","doi":"10.1093/mam/ozaf033","DOIUrl":"https://doi.org/10.1093/mam/ozaf033","url":null,"abstract":"Transmission electron microscopy is a classical methodology for diagnosing many ciliary disorders. However, heterogeneity in sampling, low yield of ciliary epithelium by cytology, improper fixation, and different orientations of cylindrical axes of cilia section create difficulties in achieving the appropriate ciliary ultrastructure for diagnosis. We have reported an improvement in the number and proportion of ciliary epithelial cells up to 32 times. These include cleaning the nose before sample collection, changing the collection brush used for the cervical cytology (eight times), and paired nose collection (four times). We have optimized the primary fixation to preserve cilia ultrastructure, modified ultra-sectioning, and improved the number of reportable ultrastructure of cilia by tilting the sample angles of α axes (up to ±40°) and β axes (up to ±25°). These optimizations resulted in ∼20 times more reportable cilia (parallel to cylindrical axes concerning the electron beam, clearly visible dynein arms, 9 + 2 patterns, and compound cilia). This resulted in an enhanced diagnosis of ciliary disorders, up to 67% (reported in 135 among 200 patients), compared with 5% achieved using conventional routine practices at our facility. With certain advancements in conventional method, we could achieve an accuracy comparable to the new sophisticated cryo-electron tomography imaging.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

4D-STEM-in-SEM: Changing an SEM Microscope to a User-friendly Powder Electron Diffractometer. 4d - stem -在扫描电镜：改变扫描电镜显微镜的用户友好的粉末电子衍射仪。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-05-09 DOI: 10.1093/mam/ozaf045

Miroslav Slouf, Pavlina Sikorova, Ewa Pavlova, Malgorzata Swietek, Lenaic Lartigue, Radim Skoupy, Vladislav Krzyzanek

{"title":"4D-STEM-in-SEM: Changing an SEM Microscope to a User-friendly Powder Electron Diffractometer.","authors":"Miroslav Slouf, Pavlina Sikorova, Ewa Pavlova, Malgorzata Swietek, Lenaic Lartigue, Radim Skoupy, Vladislav Krzyzanek","doi":"10.1093/mam/ozaf045","DOIUrl":"https://doi.org/10.1093/mam/ozaf045","url":null,"abstract":"We describe recent improvements of our method named powder nanobeam diffraction in four-dimensional scanning transmission electron microscopy (4D-STEM/PNBD). The method can change an arbitrary SEM equipped with a 2D-array STEM detector to a user-friendly powder electron diffractometer. It reduces a 4D-STEM dataset to a single 2D powder electron diffraction pattern (using our Python package named STEMDIFF; https://pypi.org/project/stemdiff) and then to 1D radially averaged diffraction profile (using our Python package named EDIFF; https://pypi.org/project/ediff). Moreover, the EDIFF package can compare the final diffractogram with theoretically calculated X-ray diffraction patterns. Both STEMDIFF and EDIFF can be used in the form of simple interactive templates in Jupyter environment, which makes them accessible to common SEM users. The recent improvements in STEMDIFF and EDIFF (better dataset filtering, parallelization, and more flexible user interface) enabled us to analyze not only strongly diffracting nanocrystals but also samples with higher absorption and/or lower diffraction power. The final results obtained from 4D-STEM/PNBD datasets of all six samples analyzed in this contribution (two types of Au nanocrystals, GdF3 and TbF3 aggregates, and Fe3O4 nanoclusters with/without organic shell) were shown to be comparable with the results of the classical TEM/SAED method (selected area electron diffraction in TEM).","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144266650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancing Atom Probe Tomography of SrTiO3: Measurement Methodology and Impurity Detection Limits. 先进的SrTiO3原子探针层析成像：测量方法和杂质检测极限。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-05-09 DOI: 10.1093/mam/ozaf051

Janina Malin Rybak, Jonas Arlt, Baptiste Gault, Cynthia A Volkert

引用次数: 0

Enhanced Prognostication of Early Breast Cancer Outcomes Using Deep Learning on Merged Multistain and Multicolor-Depth Tumor Histopathology. 利用融合多染色和多色深度肿瘤组织病理学的深度学习增强早期乳腺癌预后的预测。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-05-09 DOI: 10.1093/mam/ozaf044

Yifei Lin, Xingyu Li, Jelena Milovanović, Nataša Todorović Raković, Velicko Vranes, Tijana Vujasinović, Ksenija Kanjer, Marko Radulovic

{"title":"Enhanced Prognostication of Early Breast Cancer Outcomes Using Deep Learning on Merged Multistain and Multicolor-Depth Tumor Histopathology.","authors":"Yifei Lin, Xingyu Li, Jelena Milovanović, Nataša Todorović Raković, Velicko Vranes, Tijana Vujasinović, Ksenija Kanjer, Marko Radulovic","doi":"10.1093/mam/ozaf044","DOIUrl":"https://doi.org/10.1093/mam/ozaf044","url":null,"abstract":"Accurate breast cancer prognosis helps clinicians in selecting optimal treatments, potentially improving patient survival. We tested whether combining deep learning with tumor histopathology images could reliably predict cancer spread. Advantages of this study include the use of deep learning, which often outperforms traditional methods, and the analysis of tumor histopathology images that offer higher resolution than MRI or CT. We also optimized tumor immunostaining by separately staining slides with AE1/AE3 pan-cytokeratin and hematoxylin and eosin (H&E), and evaluated different image color-depth representations (color, grayscale, and binary) for their prognostic utility. The results indicate that grayscale images outperformed both color and binary formats. Grayscale pan-CK-stained images achieved 94.4% accuracy [area under the curve (AUC) = 0.982], while grayscale H&E-stained images reached 85.7% accuracy (AUC = 0.992) on the test set. Notably, training the ResNet-50 model with experimentally augmented data comprising six distinct datasets differing in staining type and color depth, totaling 2,646 images, further enhanced performance, to 100% accuracy (AUC of 1.0). Importantly, our pipeline ensured no contamination between the development and test sets. Deep learning applied to tumor histopathology images of early-stage breast cancer patients using two stains and varying color depths achieved exceptional prognostic accuracy and robust generalization.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing eCHORD Orientation Mapping by Means of a Derivative Approach. 利用导数方法增强回声方向映射。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-05-09 DOI: 10.1093/mam/ozaf054

Gabriel L'Hôte, Thierry Douillard, Claire Maurice, Matthieu Bugnet, Romain Haeffele, Sabrina Marcelin, Bernard Normand, Cyril Langlois

{"title":"Enhancing eCHORD Orientation Mapping by Means of a Derivative Approach.","authors":"Gabriel L'Hôte, Thierry Douillard, Claire Maurice, Matthieu Bugnet, Romain Haeffele, Sabrina Marcelin, Bernard Normand, Cyril Langlois","doi":"10.1093/mam/ozaf054","DOIUrl":"https://doi.org/10.1093/mam/ozaf054","url":null,"abstract":"The core indexing engine of the electron Channelling ORientation Determination (eCHORD) technique has been modified to significantly minimize indexation errors in the resulting orientation maps, thus enhancing accurate microstructure characterization. Orientation mapping using eCHORD involves acquiring a sequence of backscattered electron images by rotating the region of interest (ROI) within the scanning electron microscope, the sample being tilted in the range ∼10°-15°. Such an image series forms a data-cube from which intensity profiles can be extracted at each position in the ROI, reflecting the variation of the backscattered electron signal as a function of sample rotation. These profiles are compared to a database of theoretical profiles obtained by dynamical diffraction simulations. Here, the methodology consists of emphasizing the importance of diffraction peak positions in these profiles, by differentiating the data extracted from the backscattered electron images rather than using them in their raw form. The implementation of the profile derivative significantly improves the correspondence between experimental data and its theoretical counterpart. The characterization of sub-micron twin boundaries in a copper interconnect on a microelectronic chip provides insights into the effects of this indexing refinement. A comparison with Electron Back Scattered Diffraction data on a twinned nickel-based sample confirms the results.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three-dimensional Ultrastructural and Anatomical Analysis of the Relationship Between Subepithelial Stromal Cells and Shape of Basement Membrane of the Epithelium of Prostatic Duct in Mice. 小鼠前列腺导管上皮下基质细胞与基底膜形态关系的三维超微结构及解剖分析。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-05-09 DOI: 10.1093/mam/ozaf027

Kei-Ichiro Uemura, Akinobu Togo, Tasuku Hiroshige, Keisuke Ohta, Yoshihiro Kawaguchi, Kosuke Ueda, Kiyoaki Nishihara, Makoto Nakiri, Shingo Hirashima, Tsukasa Igawa, Kei-Ichiro Nakamura

{"title":"Three-dimensional Ultrastructural and Anatomical Analysis of the Relationship Between Subepithelial Stromal Cells and Shape of Basement Membrane of the Epithelium of Prostatic Duct in Mice.","authors":"Kei-Ichiro Uemura, Akinobu Togo, Tasuku Hiroshige, Keisuke Ohta, Yoshihiro Kawaguchi, Kosuke Ueda, Kiyoaki Nishihara, Makoto Nakiri, Shingo Hirashima, Tsukasa Igawa, Kei-Ichiro Nakamura","doi":"10.1093/mam/ozaf027","DOIUrl":"https://doi.org/10.1093/mam/ozaf027","url":null,"abstract":"This study examined the three-dimensional (3D) ultrastructural relationship between subepithelial stromal cells (SESCs) and the basement membranes (BM) of the prostatic epithelium in mice. Three 13-week-old male C57BL/6 mice were used for analysis of the lower urinary tract via en bloc staining as well as 3D ultrastructural analysis of SESCs, stroma, and luminal and basal epithelial cells. Morphologically, two types of epithelial BMs were observed: wavy and straight. SESCs associated with straight BMs were sheet-like in structure and much larger than those associated with the wavy BMs, with occasional protrusions extending toward the BM. SESCs with wavy BMs were of two major types: one type was sheet-like and relatively large, frequently extending its projections toward the BM, whereas the other type was small and mostly did not extend its projections toward the BM. Some SESCs with wavy and straight BMs exhibited contact with nerves. However, none of the analyzed SESCs showed vascular contact. Our study revealed the relationship between SESCs and the BM of epithelia in the mouse prostatic duct. Each SESC had a distinctive morphology that matched the shape of the epithelial BM. SESCs could be a new target for research in the fields of anatomy and physiology.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144026678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role of Laser Pulse Energy on Data Quality and Reconstruction of Olivine Atom Probe Tomography Data. 激光脉冲能量对橄榄石原子探针层析成像数据质量和重建的影响。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-05-09 DOI: 10.1093/mam/ozaf040

Dimitrios Dimitriou, Steven M Reddy, David W Saxey, Denis Fougerouse, Katy Evans, Xiao Sun, William D A Rickard

{"title":"The Role of Laser Pulse Energy on Data Quality and Reconstruction of Olivine Atom Probe Tomography Data.","authors":"Dimitrios Dimitriou, Steven M Reddy, David W Saxey, Denis Fougerouse, Katy Evans, Xiao Sun, William D A Rickard","doi":"10.1093/mam/ozaf040","DOIUrl":"https://doi.org/10.1093/mam/ozaf040","url":null,"abstract":"The study of the structure and geochemistry of olivine crystal defects is important but difficult because of their nanometer size and the analytical limitations of most techniques. Laser-assisted atom probe tomography (APT) is capable of sub-nanometer resolution, quantitative geochemical analysis and 3D reconstruction of olivine defects, but optimal analytical conditions and data reconstruction strategies have not been sufficiently studied. Here, we investigate the effect of different laser pulse energy (LPE) and crystal orientations on the quality and reconstruction parameters of APT data using specimens from two San Carlos olivine grains. Our findings show that increased LPE reduces the background noise, percentage of multiple hit events, and applied electric field, as shown by the Mg2+/Mg+ ratio, but increases the peak tails. The major element compositions show inaccuracies under all LPEs but exhibit higher consistency for higher LPEs. We determine that a LPE of 150pJ is the best compromise for optimal data quality in olivine. Using scanning electron microscopy imaging before and after APT analyses, we suggest that the Mg2+/Mg+ ratio can be used as a guide to estimate the electric field parameter and results in more accurate reconstructions.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144266651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scanning Transmission Electron Microscopy-Atom Probe Tomography Correlative Analysis for the Characterization of Solute-defect Interactions. 扫描透射电子显微镜-原子探针层析成像表征溶质缺陷相互作用的相关分析。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-05-09 DOI: 10.1093/mam/ozaf039

Siwei Chen, Jonathan D Poplawsky, Steven J Zinkle, Matthew Boebinger, Yajie Zhao

{"title":"Scanning Transmission Electron Microscopy-Atom Probe Tomography Correlative Analysis for the Characterization of Solute-defect Interactions.","authors":"Siwei Chen, Jonathan D Poplawsky, Steven J Zinkle, Matthew Boebinger, Yajie Zhao","doi":"10.1093/mam/ozaf039","DOIUrl":"https://doi.org/10.1093/mam/ozaf039","url":null,"abstract":"Atom probe tomography (APT) and (scanning) transmission electron microscopy ((S)TEM) are complementary techniques that provide spatially resolved chemical and structural information at the atomic scale. In this study, we employ two different STEM/APT correlative analysis methods to investigate Cr segregation at dislocation loops in ultra-high purity Fe-Cr alloys. APT needles for the correlative analysis were extracted either from bulk material or from thinned TEM lamellae. STEM analysis was used to determine the Burgers vectors of ion-irradiation-induced dislocation loops, while APT reconstruction of the same region revealed the Cr segregation to these loops. We extended the g•b = 0 invisibility criterion of dislocation loops from TEM mode in a lamella to STEM mode in a needle-shaped specimen. STEM and APT analysis on the same needle provide straightforward correlative analysis, although it is limited by a small observation volume. In contrast, iterative STEM analysis of TEM lamellae, followed by the selective extraction of specific regions of interest for APT analysis, expands the observation area by up to 100 times but requires additional time-consuming steps for APT needle extraction from the lamellae.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0