Microscopy and Microanalysis最新文献_第3页

3D Histological Analysis of Soft Tissues by Contrast-Enhanced X-Ray Microfocus Computed Tomography: Screening and Staining Optimization of Contrast-Enhancing Staining Agents. 对比增强x射线微聚焦计算机断层扫描软组织三维组织学分析：对比增强染色剂的筛选和染色优化。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-03-17 DOI: 10.1093/mam/ozaf013

Delia Hoffmann, Tim Balcaen, Sarah Vangrunderbeeck, Léna Puigdevall Mata, Arne Maes, Grzegorz Pyka, Laure Dumoutier, Catherine Behets, Wim De Borggraeve, Greet Kerckhofs

{"title":"3D Histological Analysis of Soft Tissues by Contrast-Enhanced X-Ray Microfocus Computed Tomography: Screening and Staining Optimization of Contrast-Enhancing Staining Agents.","authors":"Delia Hoffmann, Tim Balcaen, Sarah Vangrunderbeeck, Léna Puigdevall Mata, Arne Maes, Grzegorz Pyka, Laure Dumoutier, Catherine Behets, Wim De Borggraeve, Greet Kerckhofs","doi":"10.1093/mam/ozaf013","DOIUrl":"10.1093/mam/ozaf013","url":null,"abstract":"The gold standard for studying biological soft tissues at the microscale (i.e., histology) is tissue sectioning with subsequent colorimetric or fluorescent staining and visual inspection under the microscope. When tissue integrity must be maintained for 3D histological assessment, contrast-enhanced microfocus X-ray computed tomography (CECT) is a promising solution, but there is still a lack of staining protocol optimization of contrast-enhancing staining agents (CESAs). Therefore, in this study, mouse auricles were incubated with Hafnium-substituted Wells-Dawson polyoxometalate, cationic iodinated contrast agent, or Lugol's iodine and were imaged with high-resolution CECT. Alignment with corresponding H&E-stained sections enabled the identification and segmentation of different tissue types. Contrast differences between tissue types were increased by washing the samples after staining or by combining CESAs. Finally, we proved that the latter could be used to quantitatively assess the 3D thickness distribution of the epidermis in the ears of a mouse model of psoriasis-like dermatitis. In conclusion, CECT and bright-field microscopy are complementary and not mutually exclusive techniques for the histological assessment of biological tissues. While bright-field microscopy gives detailed information about the cellular composition of tissues, CECT provides a better insight into the spatial interrelationship of tissues and is a powerful tool for performing 3D structural quantification.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Visualizing Epigenetics: A Review of Microscopy Techniques for Investigating DNA Methylation Patterns, Chromatin Structure, and Gene Expression. 可视化表观遗传学：用于研究DNA甲基化模式、染色质结构和基因表达的显微镜技术综述。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-03-17 DOI: 10.1093/mam/ozaf017

Anna-Lee C Thompson, Judith L M Wopereis, Yonas I Tekle, Laura A Katz

{"title":"Visualizing Epigenetics: A Review of Microscopy Techniques for Investigating DNA Methylation Patterns, Chromatin Structure, and Gene Expression.","authors":"Anna-Lee C Thompson, Judith L M Wopereis, Yonas I Tekle, Laura A Katz","doi":"10.1093/mam/ozaf017","DOIUrl":"10.1093/mam/ozaf017","url":null,"abstract":"Microscopy approaches are frequently used to decipher the localization and quantify the abundance of biologically relevant molecular targets within single cells. Recent research has applied many optical imaging techniques to specifically visualize epigenetic modifications, the mechanisms by which organisms control gene expression in response to environmental factors. While many molecular and omics-based approaches are used to understand epigenetic mechanisms, imaging approaches provide spatial information that supplies greater context for discerning function. Thus, labeling approaches have been developed to quantify and visualize epigenetic targets using various fluorescence microscopy, electron microscopy, and super-resolution microscopy techniques. Here, we synthesize information about microscopy methods that enable visualization of epigenetic marks including DNA methylation, histone modifications, and localization of RNAs, which provide insights into mechanisms involved in chromatin remodeling and gene expression. The ability to determine how and where specific epigenetic marks manifest structurally and functionally in cells demonstrates the power of microscopy in aiding our understanding of epigenetic processes.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11953014/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143743001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Template Matching Approach for Automated Determination of Crystal Phase and Orientation of Grains in 4D-STEM Precession Electron Diffraction Data for Hafnium Zirconium Oxide Ferroelectric Thin Films. 模板匹配法自动测定氧化铪锆铁电薄膜4D-STEM进动电子衍射数据中的晶相和晶粒取向

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-03-17 DOI: 10.1093/mam/ozaf019

Alain C Diebold, Colin Ophus, Amir Kordijazi, Steven Consiglio, Sarah Lombardo, Dina Triyoso, Kandabara Tapily, Anna Mian, Nithin B V I Shankar, Tomáš Morávek, Narendraraj Chandran, Robert Stroud, Gert Leusink

{"title":"Template Matching Approach for Automated Determination of Crystal Phase and Orientation of Grains in 4D-STEM Precession Electron Diffraction Data for Hafnium Zirconium Oxide Ferroelectric Thin Films.","authors":"Alain C Diebold, Colin Ophus, Amir Kordijazi, Steven Consiglio, Sarah Lombardo, Dina Triyoso, Kandabara Tapily, Anna Mian, Nithin B V I Shankar, Tomáš Morávek, Narendraraj Chandran, Robert Stroud, Gert Leusink","doi":"10.1093/mam/ozaf019","DOIUrl":"https://doi.org/10.1093/mam/ozaf019","url":null,"abstract":"Thin film processing methods used to fabricate ferroelectric hafnium zirconium oxide typically result in small-grained films with a mixture of ferroelectric and nonferroelectric crystal phases with various crystallographic orientations. Although reliable, rapid determination of grain phase and orientation from four-dimensional scanning transmission electron microscopy maps is critical for measuring increased ferroelectric response, an assessment of automated analysis methods is not available. Here, a comparison of results between commercially available software (NanoMEGAS ASTAR) and an open-source code (py4DSTEM) is presented. Typically, the lamella used for STEM characterization are thicker than the average hafnium zirconium oxide (HZO) grain size, resulting in 4D maps where dynamical diffraction from more than one grain occurs in a significant number of pixels. Thus, precession electron diffraction (PED) data was required for reliable automated template matching analysis. Reliably distinguishing between the different crystal phases of HZO is challenging due to the small difference in lattice constant between phases and the possible presence of multiple orthorhombic phases. The HZO films in this study were characterized using PED, and precession diffraction simulation capability was added to py4DSTEM. Correlation of automated phase mapping with electrical verification of the ferroelectric effect confirmed the identification of the noncentrosymmetric space group 29 orthorhombic phase of HZO.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of Crepis rueppellii Leaf Extract Against Paracetamol-Inducing Nephrotoxicity in Guinea Pigs: A Histological, Immunohistochemical, and Biochemical Study. 对豚鼠扑热息痛肾毒性作用的组织学、免疫组织化学和生化研究

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-03-17 DOI: 10.1093/mam/ozaf003

Warda Mohamed Abdu Kaidama, Esam Mohammed Qasem Aqlan, Gamal H El-Sokkary, Shaimaa M M Saleh

{"title":"Role of Crepis rueppellii Leaf Extract Against Paracetamol-Inducing Nephrotoxicity in Guinea Pigs: A Histological, Immunohistochemical, and Biochemical Study.","authors":"Warda Mohamed Abdu Kaidama, Esam Mohammed Qasem Aqlan, Gamal H El-Sokkary, Shaimaa M M Saleh","doi":"10.1093/mam/ozaf003","DOIUrl":"https://doi.org/10.1093/mam/ozaf003","url":null,"abstract":"The present study aimed to evaluate the antioxidant protective effects of the herbal extract of Crepis rueppellii (CR) against paracetamol (PCM)-induced nephrotoxicity in guinea pigs using hematological, biochemical, histological, and immunohistochemical methods. Thirty guinea pigs were divided into six groups: control, PCM (2 g/kg), CR1 group (100 mg/kg), CR2 group (200 mg/kg), CR1 (100 mg/kg) + PCM, and CR2 (200 mg/kg) + PCM. The hematological results showed that the PCM-treated groups experienced significant reductions in Hb, red blood corpuscles, packed cell volume, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, white blood cell, platelets, neutrophils, lymphocyte, and monocyte, in addition to albumin and total protein levels compared with the control group. In contrast, the groups treated with CR showed increases in all the abovementioned parameters. Morphologically, kidney tissues of PCM and CR-treated groups showed focal leukocytic infiltrations and extensive necrosis, while CR1 + PCM- and CR2 + PCM-treated groups revealed more pathological changes than PCM alone. The Bax immunohistochemical reaction showed strong positive reactions in both the PCM and CR-treated groups, while the CR + PCM-treated groups displayed very strong positive compared with the PCM-only group. In conclusion, the results from the hematological and biochemical parameters indicated that CR extract had an ameliorative effect against PCM toxicity.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144007765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Maltose-based Mixtures With Lanthanoid Acetates as Electron Staining Reagents for Transmission Electron Microscopy of Extracellular Vesicles and Liposomes. 麦芽糖基混合物与类镧乙酸酯作为细胞外囊泡和脂质体透射电镜电子染色试剂的评价。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-03-17 DOI: 10.1093/mam/ozaf026

Noriyuki Ishii

{"title":"Evaluation of Maltose-based Mixtures With Lanthanoid Acetates as Electron Staining Reagents for Transmission Electron Microscopy of Extracellular Vesicles and Liposomes.","authors":"Noriyuki Ishii","doi":"10.1093/mam/ozaf026","DOIUrl":"https://doi.org/10.1093/mam/ozaf026","url":null,"abstract":"Extracellular vesicles (EVs), including exosomes, have attracted interest as vehicles for intercellular communication. Recognition of EVs as bio-derived nanocolloids allows for advanced fractionation and preparation methods that preserve their integrity. Direct observation of EVs is critical, and negative staining with heavy metal salts is a standard technique in biological transmission electron microscopy for routine morphological analysis and experimental optimization. However, customizations are often required for specific objectives. Uranyl acetate, widely used for its excellent electron scattering properties, allows clear visualization of ultrafine structures but is limited by strict global regulations and its harsh effects on biological membranes. To overcome these challenges, the author investigated lanthanoid series heavy metal salts as alternatives. Among various maltose-based mixtures, a combination of lanthanum and samarium acetates proved effective for observing EV morphology. In addition, replacing maltose with trehalose, which is known to increase water retention, resulted in improved staining characteristics with lanthanum and samarium acetates.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancing Super-Resolution Microscopy: Recent Innovations in Commercial Instruments. 推进超分辨率显微镜：商业仪器的最新创新。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-03-17 DOI: 10.1093/mam/ozaf004

Louisa Mezache, Christophe Leterrier

引用次数: 0

Effect of Intranasally Administered Stem Cell-Derived Exosomes on Rat's Olfactory Bulb Histological Structure After Lead-Oxide Nanoparticle Administration. 经鼻给药的干细胞衍生外泌体对氧化铅纳米颗粒给药后大鼠嗅球组织结构的影响。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-03-17 DOI: 10.1093/mam/ozaf008

Samaa Samir Kamar, Nevine Bahaa, Mostafa A Dabbos, Asmaa Mohammed ShamsEldeen, Walaa Baher, Abeer Attia, Lobna A Elkhateb

{"title":"Effect of Intranasally Administered Stem Cell-Derived Exosomes on Rat's Olfactory Bulb Histological Structure After Lead-Oxide Nanoparticle Administration.","authors":"Samaa Samir Kamar, Nevine Bahaa, Mostafa A Dabbos, Asmaa Mohammed ShamsEldeen, Walaa Baher, Abeer Attia, Lobna A Elkhateb","doi":"10.1093/mam/ozaf008","DOIUrl":"10.1093/mam/ozaf008","url":null,"abstract":"Lead is a common heavy metal used in a variety of products. We investigated the effect of lead-oxide nanoparticles (PbO-NPs) on the histological structure of the rat olfactory bulb (OB) and the potential therapeutic effect of stem cell-derived exosomes (EXOs). Forty male rats were included: 8 for EXO isolation and 32 were distributed into 4 groups: control, PbO-NPs group [intranasal (IN) 50 µL PbO-NPs/rat for 3 weeks], recovery group (PbO-NPs for 3 weeks and left for another 4 weeks), and PbO-NP-EXO (PbO-NPs for 3 weeks then IN EXO for another 3 weeks). After 7 weeks, olfactory behavioral tests were done before scarification. OBs were stained with hematoxylin and eosin, toluidine blue, and immunohistochemistry for synaptophysin (SYP), and ionized calcium-binding adaptor molecule-1 (Iba-1) and glial fibrillary acidic protein (GFAP). The results were confirmed by histomorphometry and statistical analysis. PbO-NPs and recovery groups showed a significant olfactory dysfunction, significant decrease in the thickness of OB layers and diameter of glomeruli, Nissl's granules of mitral cells and SYP immunostaining, and a significant increase in Iba-1 and GFAP expression compared with control rats. In PbO-NP-EXO, notable regaining of OB structure and function was obvious with reversal of most of the behavioral and morphometric findings compared with the untreated groups. IN EXO administration improved the structure and function of PbO-NP-induced OB neurotoxicity.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamics of the Posthatching Testicular Development in Japanese Quail (Coturnix coturnix japonica): Histological and Ultrastructural Study. 日本鹌鹑（Coturnix Coturnix japonica）毛后睾丸发育动态的组织学和超微结构研究。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-03-17 DOI: 10.1093/mam/ozaf012

Sara M M Eldesoky, Marwa M Hussein, Fatma M Abdel-Maksoud

{"title":"Dynamics of the Posthatching Testicular Development in Japanese Quail (Coturnix coturnix japonica): Histological and Ultrastructural Study.","authors":"Sara M M Eldesoky, Marwa M Hussein, Fatma M Abdel-Maksoud","doi":"10.1093/mam/ozaf012","DOIUrl":"https://doi.org/10.1093/mam/ozaf012","url":null,"abstract":"The posthatching development of the testis is a well-organized process comprising the maturation of Sertoli cells, the development of Leydig cells, and the differentiation of germ cells. This study aimed to investigate the posthatching testicular development in the Japanese quail, using light and electron microscope. The current study was performed on 25 healthy Japanese quail chicks at 0, 7, 21, 40, and 50 posthatching days. The results revealed that the testis consists of solid seminiferous cords, and their lining epithelium is composed of two types of cells; immature Sertoli cells and gonocyte or spermatogonia at the early stage, which begins from the day of hatching till 21 days posthatching. The interstitium during this period consisted of different developmental stages of the Leydig cells. However, at the late posthatching developmental stage the testis is characterized by the presence of round and elongated spermatids as well as the initiation of spermiogenesis. The interstitial compartment also showed an increase in the number and size of Leydig cells. The findings of the current study provide comprehensive insights into the posthatching development of the Japanese quail testis, contributing to the understanding of avian reproduction.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144022839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sample Processing and Benchmarking for Multibeam Optical Scanning Transmission Electron Microscopy. 多光束光学扫描透射电子显微镜的样品处理和基准测试。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-03-17 DOI: 10.1093/mam/ozaf024

B H Peter Duinkerken, Arent J Kievits, Anouk H G Wolters, Daan van Beijeren Bergen En Henegouwen, Jeroen Kuipers, Jacob P Hoogenboom, Ben N G Giepmans

{"title":"Sample Processing and Benchmarking for Multibeam Optical Scanning Transmission Electron Microscopy.","authors":"B H Peter Duinkerken, Arent J Kievits, Anouk H G Wolters, Daan van Beijeren Bergen En Henegouwen, Jeroen Kuipers, Jacob P Hoogenboom, Ben N G Giepmans","doi":"10.1093/mam/ozaf024","DOIUrl":"10.1093/mam/ozaf024","url":null,"abstract":"Electron microscopy (EM) is an indispensable technique to visualize biological ultrastructure in health and disease. High-throughput EM further enables larger scales and volumes to be recorded within feasible timeframes. Multibeam optical scanning transmission EM (OSTEM) utilizes multiple beamlets and optical separation of the transmitted electrons to increase imaging throughput with transmission-based imaging. However, the compatibility of multibeam OSTEM with routine sample preparation protocols and the effect of machine settings on image quality remain largely unknown. Here, we show multibeam OSTEM to be an order of magnitude faster than (scanning) transmission EM while yielding comparable high-quality images of tissue processed with standard high-contrast staining protocols. Multibeam OSTEM benefits from embedding approaches that introduce high contrast but is flexible in the type of stain used. Optimal results are obtained using an acceleration voltage of 5 kV, where section thickness and pixel dwell time require a balance between throughput and image quality. Our results show high-throughput EM with imaging quality comparable with commonly used transmission-based modalities, enabling biological ultrastructure analysis across larger scales and volumes.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mass-Thickness Measurements in the Transmission Electron Microscope: A Single-Standard Approach to Quantitative EDS Analysis. 透射电子显微镜的质量厚度测量：定量EDS分析的单一标准方法。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-03-17 DOI: 10.1093/mam/ozaf005

Thomas J Zega, Jane Y Howe, Devin L Schrader, James Sagar, Philippe Pinard, Sam Marks

引用次数: 0