Microscopy and Microanalysis最新文献_第10页

The Dynamic Atom-Probe: Past, Present, and Perspectives. 动态原子探测器：过去、现在和前景。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-02-03 DOI: 10.1093/mam/ozae115

Norbert Kruse, Thierry Visart de Bocarmé

{"title":"The Dynamic Atom-Probe: Past, Present, and Perspectives.","authors":"Norbert Kruse, Thierry Visart de Bocarmé","doi":"10.1093/mam/ozae115","DOIUrl":"10.1093/mam/ozae115","url":null,"abstract":"The present communication aims at demonstrating the wealth of information accessible by 1D-atom probe experiments using pulsed field desorption mass spectrometry (PFDMS), ultimately combined with video-field ion microscopy, while subjecting metallic samples to elevated gas pressures and studying surface reaction kinetics. Two case studies are being presented here: (a) the microkinetics of nickel tetracarbonyl (Ni(CO)4) formation through reaction of carbon monoxide with nickel and (b) the nitric oxide decomposition and reaction with hydrogen on platinum at variable steady electric fields mimicking electrocatalytic conditions. In both cases, surface areas with 140-150 atomic sites of the stepped Ni (001) and Pt (111) sample surfaces were probed. Under (a), we demonstrate variable repetition frequencies of field pulses to inform kinetic and mechanistic details of the surface reaction while under (b), we reveal the occurrence of field-induced processes impacting the surface reaction mechanism of nitric oxide with hydrogen and therefore opening new pathways not available under purely thermal conditions (in the absence of electric fields). Some aspects of PFDMS technical achievements will be discussed as they may provide clues for designing dynamic atom probe tomography instrumentation.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":" ","pages":"1100-1108"},"PeriodicalIF":2.9,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142751272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of Parameters for Atom Probe Tomography Analysis of β-Tricalcium Phosphates. β-磷酸三钙原子探针断层扫描分析参数的优化。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-02-03 DOI: 10.1093/mam/ozae077

Selase Torkornoo, Marc Bohner, Ingrid McCarroll, Baptiste Gault

引用次数: 0

Decreased in Mitochondrial Complex I Subunit NDUFS2 Is Critical for Oocyte Quality During Postovulatory Aging in Pigs. 线粒体复合体 I 亚基 NDUFS2 的减少对猪排卵后衰老过程中的卵母细胞质量至关重要

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-11-04 DOI: 10.1093/mam/ozae079

Dongjie Zhou, Song-Hee Lee, Xiao-Han Li, Ji-Dam Kim, Gyu-Hyun Lee, Jae-Min Sim, Xiang-Shun Cui

{"title":"Decreased in Mitochondrial Complex I Subunit NDUFS2 Is Critical for Oocyte Quality During Postovulatory Aging in Pigs.","authors":"Dongjie Zhou, Song-Hee Lee, Xiao-Han Li, Ji-Dam Kim, Gyu-Hyun Lee, Jae-Min Sim, Xiang-Shun Cui","doi":"10.1093/mam/ozae079","DOIUrl":"10.1093/mam/ozae079","url":null,"abstract":"The levels of nicotinamide adenine dinucleotide (NADH) dehydrogenase [ubiquinone] iron-sulfur protein 2 (NDUFS2, a subunit of NADH dehydrogenase) decrease in aged tissues, and these reductions may be partly associated with age-related conditions such as Parkinson's disease. Aging leads to many mitochondrial defects, such as biogenesis disruption, dysfunction, defects in the mitochondrial membrane potential, and production of reactive oxygen species, that may be highly related to NDUFS2 expression. The relationship between NDUFS2 and postovulatory oocyte aging in pigs remains unknown. In this study, we investigated changes in NDUFS2 expression during postovulatory aging (POA). Furthermore, NDUFS2 was knocked down via dsRNA microinjection at the MII stage to evaluate the effects on mitochondrial-related processes during POA. The mRNA expression of NDUFS2 decreased significantly after 48-h aging compared with that in fresh oocytes. NDUFS2 knockdown (KD) significantly impaired the maintenance of oocyte morphology and blastocyst development of embryos after POA. The levels of PGC1α (mitochondrial biogenesis-related proteins) decreased significantly after NDUFS2 KD, while the level of GSNOR, a protein denitrosylase, was reduced by NDUFS2 KD after 48 h of aging. These data suggest that NDUFS2 is vital for maintaining the oocyte quality during POA in pigs.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":" ","pages":"953-961"},"PeriodicalIF":2.9,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142120225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Concept of Local Coordination Number for the Characterization of Solute Clusters within Atom Probe Tomography Data. 用于描述原子探针断层成像数据中溶质团簇特征的局部配位数概念。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-11-04 DOI: 10.1093/mam/ozae074

Mykola Lazarev, John Banhart

{"title":"A Concept of Local Coordination Number for the Characterization of Solute Clusters within Atom Probe Tomography Data.","authors":"Mykola Lazarev, John Banhart","doi":"10.1093/mam/ozae074","DOIUrl":"10.1093/mam/ozae074","url":null,"abstract":"Identifying clusters of solute atoms in a matrix of solvent atoms helps to understand precipitation phenomena in alloys, for example, during the age hardening of certain aluminum alloys. Atom probe tomography datasets can deliver such information, provided that appropriate cluster identification routines are available. We investigate algorithms based on the local composition of the neighborhood of solute atoms and compare them with traditional approaches based on the local solute number density, such as the maximum separation distance method. For an ideal solid solution, the pair correlation functions of the kth nearest solute atom in the coordination number representation are derived, and the percolation threshold and the size distribution of clusters are studied. A criterion for selecting optimal control parameters based on maximizing the phase separation by the degree of clustering is proposed for a two-phase system. A map of phase compositions accessible for cluster analysis is constructed. The coordination number approach reduces the influence of density variations commonly observed in atom probe tomography data. Finally, a practical cluster analysis technique applied to the early stages of aluminum alloy aging is described.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":" ","pages":"793-806"},"PeriodicalIF":2.9,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141897775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Induction of Heat Shock Proteins 27, 60, 70, and 90 in the Cerebellum of Rats After Hyperthermia During Postnatal Development. 大鼠小脑在出生后发育过程中受到高热后热休克蛋白 27、60、70 和 90 的诱导

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-11-04 DOI: 10.1093/mam/ozae075

Banu Kandil, Alev Gürol Bayraktaroglu

{"title":"Induction of Heat Shock Proteins 27, 60, 70, and 90 in the Cerebellum of Rats After Hyperthermia During Postnatal Development.","authors":"Banu Kandil, Alev Gürol Bayraktaroglu","doi":"10.1093/mam/ozae075","DOIUrl":"10.1093/mam/ozae075","url":null,"abstract":"Heat shock proteins (HSPs) are induced in response to stressful stimuli and play an important role in cell repair and protection. This study, using immunohistochemistry, aimed to determine whether HSPs are induced in the cerebellum of rats subjected to hyperthermia during postnatal development (PND). The results showed that unlike HSP27 and HSP70, HSP60 and HSP90 were constitutively expressed in the cerebellum of rats. However, hyperthermia induced HSP27 in the white matter (WM) and HSP70 in the Bergmann glial cells, the internal granule layer (IGL), and the WM. In the WM, HSP27 induction was only observed on days PND20, PND25, and PND30, and HSP27 expression was higher on day PND30 compared with days PND20 and PND25 (p < 0.001). In the Bergmann glial cells, HSP70 induction was only observed on days PND5, PND10, and PND20, and HSP70 expression was greater on days PND5 and PND10 compared with day PND20 (p < 0.001). In the IGL and the WM, HSP70 expression was higher on days PND20, PND25, and PND30 compared with days PND5 and PND10 (p < 0.001). These findings indicate that unlike HSP60 and HSP90, HSP27 and HSP70 have different expression patterns in the cerebellum of rats after hyperthermia during PND.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":" ","pages":"944-952"},"PeriodicalIF":2.9,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective Role of Melatonin Against Abamectin-Induced Biochemical, Immunohistochemical, and Ultrastructural Alterations in the Testicular Tissues of Rats. 褪黑素对阿维菌素诱导的大鼠睾丸组织生化、免疫组织化学和超微结构改变的保护作用

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-11-04 DOI: 10.1093/mam/ozae080

Caglar Adiguzel, Hatice Karaboduk, Meltem Uzunhisarcikli

{"title":"Protective Role of Melatonin Against Abamectin-Induced Biochemical, Immunohistochemical, and Ultrastructural Alterations in the Testicular Tissues of Rats.","authors":"Caglar Adiguzel, Hatice Karaboduk, Meltem Uzunhisarcikli","doi":"10.1093/mam/ozae080","DOIUrl":"10.1093/mam/ozae080","url":null,"abstract":"Abamectin is one of the most widely used pesticides due to its strong insecticidal and anthelmintic activities. Melatonin is a neurohormone with potent antioxidant, anti-apoptotic, and anti-inflammatory effects. This study aimed to investigate the potential ameliorative effects of melatonin against abamectin-induced testicular toxicity in rats. Twenty-four rats were divided into four groups: control group (1 mL/kg/day corn oil), melatonin-treated group (10 mg/kg/day), abamectin-treated group (0.5 mg/kg/day), and melatonin plus abamectin-treated group. Test substances were administered via oral gavage once daily for 28 days. While MDA and 8-OHdG levels increased in the testicular tissue of rats treated with abamectin, SOD, CAT, GPx, and GST enzyme activities decreased significantly. While interleukin-17 levels, TNF-α, and caspase3 expression increased in the testicular tissue, acetylcholinesterase activity decreased. At the same time, serum gonadotropins (luteinizing and follicle-stimulating hormones) and testosterone levels decreased. Light microscope examinations of testicular tissues revealed severe histopathological changes, such as atrophic hyalinized seminiferous tubules, basement membrane irregularity, degeneration, spermatogenic cell loss, and necrosis. Electron microscopy examinations revealed large vacuoles in Sertoli and spermatogenic cells, swelling and vacuolization in mitochondria, lysosomal structures, and increased pyknotic nuclei. In contrast, melatonin supplementation significantly ameliorated abamectin-induced testicular toxicity in rats through antioxidant, antiapoptotic, and anti-inflammatory mechanisms.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":" ","pages":"962-977"},"PeriodicalIF":2.9,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three-Dimensional Nanoscale Imaging of SiO2 Nanofiller in Styrene-Butadiene Rubber with High-Resolution and High-Sensitivity Ptychographic X-ray Computed Tomography. 利用高分辨率和高灵敏度 X 射线计算机断层扫描对苯乙烯-丁二烯橡胶中的 SiO2 纳米填料进行三维纳米级成像。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-11-04 DOI: 10.1093/mam/ozae094

Naru Okawa, Nozomu Ishiguro, Shuntaro Takazawa, Hideshi Uematsu, Yuhei Sasaki, Masaki Abe, Kyosuke Ozaki, Yoshiaki Honjo, Haruki Nishino, Yasumasa Joti, Takaki Hatsui, Yukio Takahashi

引用次数: 0

Imaging and Segmenting Grains and Subgrains Using Backscattered Electron Techniques. 利用背散射电子技术对晶粒和子晶粒进行成像和细分。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-11-04 DOI: 10.1093/mam/ozae092

Thomas J Bennett, Eric M Taleff

引用次数: 0

Wave Optical Modeling of the SEM Column From Source to Specimen. 扫描电镜柱从光源到试样的波光学建模。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-11-04 DOI: 10.1093/mam/ozae072

Surya Kamal, Yongjian Zhou, Zizhou Gong

{"title":"Wave Optical Modeling of the SEM Column From Source to Specimen.","authors":"Surya Kamal, Yongjian Zhou, Zizhou Gong","doi":"10.1093/mam/ozae072","DOIUrl":"10.1093/mam/ozae072","url":null,"abstract":"Probe formation in scanning electron microscope (SEM) is often reduced to objective lens action modeling based on a point-spread function or Fourier transforms. In this study, we present the first complete wave optical modeling of the whole SEM column based on plane-by-plane propagation of the electron beam wavefunction without simplifying the optical system. We identify the challenges in plane-by-plane beam propagation and show how sampling limitations produce aliased results. Through a careful selection and combination of propagators, we have developed a general wave optical propagation method that is able to overcome the aliasing problem to achieve the appropriate probe widths. Using a two-step propagator, we show that it is possible to model the electron beam distribution throughout the column from the virtual source plane to the specimen plane. We also show that our results from the wave optical simulations are consistent with the geometrical theory of probe formation. Finally, as a direct application of this method, we demonstrated that the combined effect of aberrations in the condenser lens and the probe forming objective lens cannot be accurately represented using only the objective lens. Designing beam shaping experiments and studying the effect of partial coherence can be some novel applications.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":" ","pages":"866-877"},"PeriodicalIF":2.9,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142000391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Carbide Precipitation in Low-Carbon Martensitic Steels Using an Ultrawide Field-of-View 3D Atom Probe. 使用超宽视场 3D 原子探针表征低碳马氏体钢中的碳化物析出。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-11-04 DOI: 10.1093/mam/ozae084

Hidekazu Minami, Levi Tegg, Takanori Sato, Julie M Cairney

{"title":"Characterization of Carbide Precipitation in Low-Carbon Martensitic Steels Using an Ultrawide Field-of-View 3D Atom Probe.","authors":"Hidekazu Minami, Levi Tegg, Takanori Sato, Julie M Cairney","doi":"10.1093/mam/ozae084","DOIUrl":"10.1093/mam/ozae084","url":null,"abstract":"It is important to understand the carbide distribution around high-energy sites such as dislocations and grain boundaries in martensitic steels as they have a major influence on the alloy performance. The aim of this study is to characterize fine ε carbides precipitated in low-carbon lath martensitic steel using the ultrawide field-of-view (FoV) CAMECA Invizo 6000 atom probe. We demonstrate the advantages of the wide FoV and determine the optimum conditions for analysis, by comparing the results such as the background noise and the C++/C+ charge state ratio (CSR) between voltage-pulsed and laser-pulsed modes. Increasing the laser pulse energy decreased the background noise and the CSR, where 70 pJ laser pulse energy produced a comparable mass-to-charge ratio spectrum to that recorded in voltage-pulsed mode, with the bulk compositions of C, Si, and Mn closest to that measured using voltage-pulsed mode. Increasing laser pulse energies to above 300 pJ decreased the bulk carbon content, with a more diffuse distribution of carbon around the carbides. This paper outlines some of the important experimental considerations when performing quantitative study of carbide precipitation in low-carbon martensitic steels using the Invizo 6000, considerations that can also be applied to other ferrous and non-ferrous alloy systems.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":" ","pages":"825-835"},"PeriodicalIF":2.9,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142126179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0