MicrobiologyOpen最新文献_第6页

Transcriptomics-guided identification of an algicidal protease of the marine bacterium Kordia algicida OT-1 转录组学引导鉴定海洋细菌褐藻Kordia algicida OT-1的一种杀藻蛋白酶

IF 3.4 3区生物学

MicrobiologyOpen Pub Date : 2023-10-10 DOI: 10.1002/mbo3.1387

Kristy S. Syhapanha, David A. Russo, Yun Deng, Nils Meyer, Remington X. Poulin, Georg Pohnert

{"title":"Transcriptomics-guided identification of an algicidal protease of the marine bacterium Kordia algicida OT-1","authors":"Kristy S. Syhapanha, David A. Russo, Yun Deng, Nils Meyer, Remington X. Poulin, Georg Pohnert","doi":"10.1002/mbo3.1387","DOIUrl":"https://doi.org/10.1002/mbo3.1387","url":null,"abstract":"In recent years, interest in algicidal bacteria has risen due to their ecological importance and their potential as biotic regulators of harmful algal blooms. Algicidal bacteria shape the plankton communities of the oceans by inhibiting or lysing microalgae and by consuming the released nutrients. Kordia algicida strain OT-1 is a model marine algicidal bacterium that was isolated from a bloom of the diatom Skeletonema costatum. Previous work has suggested that algicidal activity is mediated by secreted proteases. Here, we utilize a transcriptomics-guided approach to identify the serine protease gene KAOT1_RS09515, hereby named alpA1 as a key element in the algicidal activity of K. algicida. The protease AlpA1 was expressed and purified from a heterologous host and used in in vitro bioassays to validate its activity. We also show that K. algicida is the only algicidal species within a group of four members of the Kordia genus. The identification of this algicidal protease opens the possibility of real-time monitoring of the ecological impact of algicidal bacteria in natural phytoplankton blooms.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"12 5","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.1387","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50148209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between the Rod complex and peptidoglycan structure in Escherichia coli 大肠杆菌杆状复合体与肽聚糖结构的关系

IF 3.4 3区生物学

MicrobiologyOpen Pub Date : 2023-10-09 DOI: 10.1002/mbo3.1385

Risa Ago, Yuhei O. Tahara, Honoka Yamaguchi, Motoya Saito, Wakana Ito, Kaito Yamasaki, Taishi Kasai, Sho Okamoto, Taiki Chikada, Taku Oshima, Issey Osaka, Makoto Miyata, Hironori Niki, Daisuke Shiomi

{"title":"Relationship between the Rod complex and peptidoglycan structure in Escherichia coli","authors":"Risa Ago, Yuhei O. Tahara, Honoka Yamaguchi, Motoya Saito, Wakana Ito, Kaito Yamasaki, Taishi Kasai, Sho Okamoto, Taiki Chikada, Taku Oshima, Issey Osaka, Makoto Miyata, Hironori Niki, Daisuke Shiomi","doi":"10.1002/mbo3.1385","DOIUrl":"https://doi.org/10.1002/mbo3.1385","url":null,"abstract":"Peptidoglycan for elongation in Escherichia coli is synthesized by the Rod complex, which includes RodZ. Although various mutant strains of the Rod complex have been isolated, the relationship between the activity of the Rod complex and the overall physical and chemical structures of the peptidoglycan have not been reported. We constructed a RodZ mutant, termed RMR, and analyzed the growth rate, morphology, and other characteristics of cells producing the Rod complexes containing RMR. The growth and morphology of RMR cells were abnormal, and we isolated suppressor mutants from RMR cells. Most of the suppressor mutations were found in components of the Rod complex, suggesting that these suppressor mutations increase the integrity and/or the activity of the Rod complex. We purified peptidoglycan from wild-type, RMR, and suppressor mutant cells and observed their structures in detail. We found that the peptidoglycan purified from RMR cells had many large holes and different compositions of muropeptides from those of WT cells. The Rod complex may be a determinant not only for the whole shape of peptidoglycan but also for its highly dense structure to support the mechanical strength of the cell wall.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"12 5","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.1385","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50145344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic interactions between Candida albicans and different streptococcal species in a multispecies oral biofilm 多菌种口腔生物膜中白色念珠菌与不同链球菌的动态相互作用

IF 3.4 3区生物学

MicrobiologyOpen Pub Date : 2023-10-03 DOI: 10.1002/mbo3.1381

Tenzin Kunchok Lueyar, Lamprini Karygianni, Thomas Attin, Thomas Thurnheer

{"title":"Dynamic interactions between Candida albicans and different streptococcal species in a multispecies oral biofilm","authors":"Tenzin Kunchok Lueyar, Lamprini Karygianni, Thomas Attin, Thomas Thurnheer","doi":"10.1002/mbo3.1381","DOIUrl":"https://doi.org/10.1002/mbo3.1381","url":null,"abstract":"The oral cavity is colonized by a plethora of bacteria, fungi, and archaea, including streptococci of the mitis group (MSG) and the yeast Candida albicans. This study aims to investigate the role of streptococcal species in the development of oral biofilm and the cross-kingdom interactions between some of the members of the commensal MSG and the pathogen yeast C. albicans using a multispecies supragingival biofilm model. A total of nine different in vitro biofilms were grown, quantified with culture analyses, and visually examined with confocal laser scanning microscopy (CLSM). A four-species biofilm without any streptococcal species was used as a basic biofilm. In each subsequent inoculum, one species of MSG was added and afterward combined with Streptococcus mutans. The eight-species biofilm contained all eight strains used in this study. Culture analyses showed that the presence of S. mutans in a four-species biofilm with Streptococcus oralis or S. oralis subsp. tigurinus did not differ significantly in C. albicans colony-forming unit (CFU) counts compared to biofilms without S. mutans. However, compared to other mitis species, Streptococcus gordonii combined with S. mutans resulted in the lowest CFUs of C. albicans. Visual observation by CLSM showed that biofilms containing both S. mutans and one species of MSG seemed to induce the formation of filamentous form of C. albicans. However, when several species of MSG were combined with S. mutans, C. albicans was again found in its yeast form.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"12 5","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.1381","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50119393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Similarity in milk microbiota in replicates 复制品中牛奶微生物群的相似性

IF 3.4 3区生物学

MicrobiologyOpen Pub Date : 2023-09-30 DOI: 10.1002/mbo3.1383

Josef Dahlberg, Erik Pelve, Johan Dicksved

{"title":"Similarity in milk microbiota in replicates","authors":"Josef Dahlberg, Erik Pelve, Johan Dicksved","doi":"10.1002/mbo3.1383","DOIUrl":"https://doi.org/10.1002/mbo3.1383","url":null,"abstract":"Receiving the same results from repeated analysis of the same sample is a basic principle in science. The inability to reproduce previously published results has led to discussions of a reproducibility crisis within science. For studies of microbial communities, the problem of reproducibility is more pronounced and has, in some fields, led to a discussion on the very existence of a constantly present microbiota. In this study, DNA from 44 bovine milk samples were extracted twice and the V3–V4 region of the 16S rRNA gene was sequenced in two separate runs. The FASTQ files from the two data sets were run through the same bioinformatics pipeline using the same settings and results from the two data sets were compared. Milk samples collected maximally 2 h apart were used as replicates and permitted comparisons to be made within the same run. Results show a significant difference in species richness between the two sequencing runs although Shannon and Simpson's diversity was the same. Multivariate analyses of all samples demonstrate that the sequencing run was a driver for variation. Direct comparison of similarity between samples and sequencing run showed an average similarity of 42%–45% depending on whether binary or abundance-based similarity indices were used. Within-run comparisons of milk samples collected maximally 2 h apart showed an average similarity of 39%–47% depending on the similarity index used and that similarity differed significantly between runs. We conclude that repeated DNA extraction and sequencing significantly can affect the results of a low microbial biomass microbiota study.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"12 5","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.1383","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50155966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A loophole in soap dispensers mediates contamination with Gram-negative bacteria 肥皂分配器中的一个漏洞介导了革兰氏阴性菌的污染

IF 3.4 3区生物学

MicrobiologyOpen Pub Date : 2023-09-29 DOI: 10.1002/mbo3.1384

Ralf Lucassen, Nicole van Leuven, Dirk Bockmühl

{"title":"A loophole in soap dispensers mediates contamination with Gram-negative bacteria","authors":"Ralf Lucassen, Nicole van Leuven, Dirk Bockmühl","doi":"10.1002/mbo3.1384","DOIUrl":"https://doi.org/10.1002/mbo3.1384","url":null,"abstract":"Liquid soap dispensers are widely used in domestic and clinical settings. In previous studies, the risk of bacterial contamination of refillable systems was pointed out and a bacterial contamination rate of 25%, with values of up to 108 colony-forming units/mL (CFU/mL), was reported. However, the route of contamination remains elusive. To address this point, we determined the microbial contamination of refillable standard pump dispensers and nonrefillable press-dispenser systems. Following the collection of 104 liquid soap dispensers from hotel rooms across Germany, bacterial counts were determined. Isolates of samples containing nonfastidious Gram-negative(lac−) bacteria were further analyzed by the Vitek 2 system for the determination of species. 70.2% of the refillable pump dispensers (mean total bacterial count = 2.2 × 105 CFU/mL) but only 10.6% of the nonrefillable press dispensers, were contaminated (mean total bacterial count = 1.5 × 101 CFU/mL). Of samples containing nonfastidious Gram-negative(lac−) bacteria, Pluralibacter gergoviae was present in 41.7%, Pseudomonads (Pseudomonas aeruginosa and Pseudomonas putida) in 25%, Serratia marcescens in 16.7%, and Klebsiella oxytoca and Pasteurella testudinis in 8.3%. After the initial assessment, we contaminated different dispensing systems with P. aeruginosa/P. gergoviae, to reveal the route of contamination and identied the pressure release of standard pump dispensers as the loophole for microbial contamination.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"12 5","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.1384","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50147914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of light conditions on the production of chrysolaminarin using Phaeodactylum tricornutum in artificially illuminated photobioreactors 光照条件对利用三角褐指藻在人工光生物反应器中生产黄昆布的影响

IF 3.4 3区生物学

MicrobiologyOpen Pub Date : 2023-09-18 DOI: 10.1002/mbo3.1378

Konstantin Frick, Tobias Ebbing, Yen-Cheng Yeh, Ulrike Schmid-Staiger, Günter E. M. Tovar

{"title":"Influence of light conditions on the production of chrysolaminarin using Phaeodactylum tricornutum in artificially illuminated photobioreactors","authors":"Konstantin Frick, Tobias Ebbing, Yen-Cheng Yeh, Ulrike Schmid-Staiger, Günter E. M. Tovar","doi":"10.1002/mbo3.1378","DOIUrl":"https://doi.org/10.1002/mbo3.1378","url":null,"abstract":"The light conditions are of utmost importance in any microalgae production process especially involving artificial illumination. This also applies to a chrysolaminarin (soluble 1,3-β-glucan) production process using the diatom Phaeodactylum tricornutum. Here we examine the influence of the amount of light per gram biomass (specific light availability) and the influence of two different biomass densities (at the same amount of light per gram biomass) on the accumulation of the storage product chrysolaminarin during nitrogen depletion in artificially illuminated flat-panel airlift photobioreactors. Besides chrysolaminarin, other compounds (fucoxanthin, fatty acids used for energy storage [C16 fatty acids], and eicosapentaenoic acid) are regarded as well. Our results show that the time course of C-allocation between chrysolaminarin and fatty acids, serving as storage compounds, is influenced by specific light availability and cell concentration. Furthermore, our findings demonstrate that with increasing specific light availability, the maximal chrysolaminarin content increases. However, this effect is limited. Beyond a certain specific light availability (here: 5 µmolphotons gDW−1 s−1) the maximal chrysolaminarin content no longer increases, but the rate of increase becomes faster. Furthermore, the conversion of light to chrysolaminarin is best at the beginning of nitrogen depletion. Additionally, our results show that a high biomass concentration has a negative effect on the maximal chrysolaminarin content, most likely due to the occurring self-shading effects.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"12 5","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.1378","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50152216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial surface-exposed lipoproteins and sortase-mediated anchored cell surface proteins in plant infection 植物感染中细菌表面暴露的脂蛋白和分选酶介导的锚定细胞表面蛋白

IF 3.4 3区生物学

MicrobiologyOpen Pub Date : 2023-09-14 DOI: 10.1002/mbo3.1382

Andrés de Sandozequi, Claudia Martínez-Anaya

{"title":"Bacterial surface-exposed lipoproteins and sortase-mediated anchored cell surface proteins in plant infection","authors":"Andrés de Sandozequi, Claudia Martínez-Anaya","doi":"10.1002/mbo3.1382","DOIUrl":"https://doi.org/10.1002/mbo3.1382","url":null,"abstract":"The bacterial cell envelope is involved in all stages of infection and the study of its components and structures is important to understand how bacteria interact with the extracellular milieu. Thanks to new techniques that focus on identifying bacterial surface proteins, we now better understand the specific components involved in host–pathogen interactions. In the fight against the deleterious effects of pathogenic bacteria, bacterial surface proteins (at the cell envelope) are important targets as they play crucial roles in the colonization and infection of host tissues. These surface proteins serve functions such as protection, secretion, biofilm formation, nutrient intake, metabolism, and virulence. Bacteria use different mechanisms to associate proteins to the cell surface via posttranslational modification, such as the addition of a lipid moiety to create lipoproteins and attachment to the peptidoglycan layer by sortases. In this review, we focus on these types of proteins (and provide examples of others) that are associated with the bacterial cell envelope by posttranslational modifications and their roles in plant infection.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"12 5","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.1382","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50133176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Orientia tsutsugamushi: A life between escapes 恙虫病东方体：逃亡之间的生活

IF 3.4 3区生物学

MicrobiologyOpen Pub Date : 2023-09-10 DOI: 10.1002/mbo3.1380

Lea Fromm, Jonas Mehl, Christian Keller

引用次数: 0

Rifampicin-resistant RpoB S522L Vibrio vulnificus exhibits disturbed stress response and hypervirulence traits 抗利福平的RpoB S522L创伤弧菌表现出应激反应紊乱和高通气特性

IF 3.4 3区生物学

MicrobiologyOpen Pub Date : 2023-09-10 DOI: 10.1002/mbo3.1379

Laura Cutugno, Conor O'Byrne, Jan Pané-Farré, Aoife Boyd

引用次数: 0

Evaluating models and assessment techniques for understanding oral biofilm complexity 了解口腔生物膜复杂性的评估模型和评估技术。

IF 3.4 3区生物学

MicrobiologyOpen Pub Date : 2023-08-29 DOI: 10.1002/mbo3.1377

Srinivas Sulugodu Ramachandra, Patricia Wright, Pingping Han, Abdalla Abdal-hay, Ryan S. B. Lee, Saso Ivanovski

{"title":"Evaluating models and assessment techniques for understanding oral biofilm complexity","authors":"Srinivas Sulugodu Ramachandra, Patricia Wright, Pingping Han, Abdalla Abdal-hay, Ryan S. B. Lee, Saso Ivanovski","doi":"10.1002/mbo3.1377","DOIUrl":"10.1002/mbo3.1377","url":null,"abstract":"Oral biofilms are three-dimensional (3D) complex entities initiating dental diseases and have been evaluated extensively in the scientific literature using several biofilm models and assessment techniques. The list of biofilm models and assessment techniques may overwhelm a novice biofilm researcher. This narrative review aims to summarize the existing literature on biofilm models and assessment techniques, providing additional information on selecting an appropriate model and corresponding assessment techniques, which may be useful as a guide to the beginner biofilm investigator and as a refresher to experienced researchers. The review addresses previously established 2D models, outlining their advantages and limitations based on the growth environment, availability of nutrients, and the number of bacterial species, while also exploring novel 3D biofilm models. The growth of biofilms on clinically relevant 3D models, particularly melt electrowritten fibrous scaffolds, is discussed with a specific focus that has not been previously reported. Relevant studies on validated oral microcosm models that have recently gaining prominence are summarized. The review analyses the advantages and limitations of biofilm assessment methods, including colony forming unit culture, crystal violet, 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt assays, confocal microscopy, fluorescence in situ hybridization, scanning electron microscopy, quantitative polymerase chain reaction, and next-generation sequencing. The use of more complex models with advanced assessment methodologies, subject to the availability of equipment/facilities, may help in developing clinically relevant biofilms and answering appropriate research questions.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"12 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.1377","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10120492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1