养老院中的嗜麦芽霉单胞菌对多种药物产生耐药性:新出现的威胁

IF 3.9 3区 生物学 Q2 MICROBIOLOGY
MicrobiologyOpen Pub Date : 2024-04-29 DOI:10.1002/mbo3.1409
Sylvia A. Sapula, Bradley J. Hart, Naomi L. Siderius, Anteneh Amsalu, Jack M. Blaikie, Henrietta Venter
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引用次数: 0

摘要

嗜麦芽血单胞菌是一种耐多药(MDR)革兰氏阴性细菌,对β-内酰胺类药物(包括最后的碳青霉烯类)具有内在耐药性。作为一种机会性病原体,它可引起严重的医疗相关感染。本研究评估了从养老院(RACF)中分离出的嗜麦芽糖酵母菌的流行率、耐药性特征和遗传多样性。众所周知,养老院过度使用抗生素,而且往往使用不当,这就形成了一个有利于细菌耐药性发展的强大选择性环境。这项研究针对从三家 RACF 和一家退休村的废水和设施拭子样本中分离出的 73 个嗜麦芽汁酵母菌进行了研究。对分离菌株的表型和基因型评估显示,这些菌株对碳青霉烯类耐药性较高,体现了其固有的β-内酰胺耐药性。令人担忧的是,49.3%(36/73)的分离株对可乐定具有非野生型耐药性,最低抑菌浓度值为 4 毫克/升,11.0%(8/73)的分离株对三甲双胍-磺胺甲噁唑具有耐药性。对这两种抗菌素均未发现耐药机制。对已知菌系的基因型评估显示,分离物与 Sm17 和 Sm18 菌系聚集在一起,而这两个菌系以前在澳大利亚未曾报道过,这表明嗜麦芽糖酵母菌的 MDR 有可能正在蔓延。最后,虽然只有少数分离物对杀菌剂有耐受性(2.7%,2/73),但它们在高浓度(64 毫克/升)三氯生中的生长能力令人担忧,因为这可能会促进它们的生存和继续传播。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Multidrug-resistant Stenotrophomonas maltophilia in residential aged care facilities: An emerging threat

Multidrug-resistant Stenotrophomonas maltophilia in residential aged care facilities: An emerging threat

Stenotrophomonas maltophilia is a multidrug-resistant (MDR), Gram-negative bacterium intrinsically resistant to beta-lactams, including last-resort carbapenems. As an opportunistic pathogen, it can cause serious healthcare-related infections. This study assesses the prevalence, resistance profiles, and genetic diversity of S. maltophilia isolated from residential aged care facilities (RACFs). RACFs are known for their overuse and often inappropriate use of antibiotics, creating a strong selective environment that favors the development of bacterial resistance. The study was conducted on 73 S. maltophilia isolates recovered from wastewater and facility swab samples obtained from three RACFs and a retirement village. Phenotypic and genotypic assessments of the isolates revealed high carbapenem resistance, exemplifying their intrinsic beta-lactam resistance. Alarmingly, 49.3% (36/73) of the isolates were non-wild type for colistin, with minimum inhibitory concentration values of > 4 mg/L, and 11.0% (8/73) were resistant to trimethoprim-sulfamethoxazole. No resistance mechanisms were detected for either antimicrobial. Genotypic assessment of known lineages revealed isolates clustering with Sm17 and Sm18, lineages not previously reported in Australia, suggesting the potential ongoing spread of MDR S. maltophilia. Lastly, although only a few isolates were biocide tolerant (2.7%, 2/73), their ability to grow in high concentrations (64 mg/L) of triclosan is concerning, as it may be selecting for their survival and continued dissemination.

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来源期刊
MicrobiologyOpen
MicrobiologyOpen MICROBIOLOGY-
CiteScore
8.00
自引率
0.00%
发文量
78
审稿时长
20 weeks
期刊介绍: MicrobiologyOpen is a peer reviewed, fully open access, broad-scope, and interdisciplinary journal delivering rapid decisions and fast publication of microbial science, a field which is undergoing a profound and exciting evolution in this post-genomic era. The journal aims to serve the research community by providing a vehicle for authors wishing to publish quality research in both fundamental and applied microbiology. Our goal is to publish articles that stimulate discussion and debate, as well as add to our knowledge base and further the understanding of microbial interactions and microbial processes. MicrobiologyOpen gives prompt and equal consideration to articles reporting theoretical, experimental, applied, and descriptive work in all aspects of bacteriology, virology, mycology and protistology, including, but not limited to: - agriculture - antimicrobial resistance - astrobiology - biochemistry - biotechnology - cell and molecular biology - clinical microbiology - computational, systems, and synthetic microbiology - environmental science - evolutionary biology, ecology, and systematics - food science and technology - genetics and genomics - geobiology and earth science - host-microbe interactions - infectious diseases - natural products discovery - pharmaceutical and medicinal chemistry - physiology - plant pathology - veterinary microbiology We will consider submissions across unicellular and cell-cluster organisms: prokaryotes (bacteria, archaea) and eukaryotes (fungi, protists, microalgae, lichens), as well as viruses and prions infecting or interacting with microorganisms, plants and animals, including genetic, biochemical, biophysical, bioinformatic and structural analyses. The journal features Original Articles (including full Research articles, Method articles, and Short Communications), Commentaries, Reviews, and Editorials. Original papers must report well-conducted research with conclusions supported by the data presented in the article. We also support confirmatory research and aim to work with authors to meet reviewer expectations. MicrobiologyOpen publishes articles submitted directly to the journal and those referred from other Wiley journals.
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