Microbes and Environments最新文献

{"title":"Current Understanding of Taxonomy and Ecology of the Phylum Minisyncoccota.","authors":"Naoki Fujii, Meri Nakajima, Takashi Narihiro, Kyohei Kuroda, Tomonori Kindaichi","doi":"10.1264/jsme2.ME25084","DOIUrl":"10.1264/jsme2.ME25084","url":null,"abstract":"<p><p>The phylum Minisyncoccota (formerly known as \"Candidatus Patescibacteria\"/candidate phyla radiation [CPR] and designated under SeqCode as Patescibacteriota) represents one of the major bacterial phyla; however, its physiological and ecological characteristics remain unclear. This review summarizes relevant studies on currently available isolate and genomic/metagenomic data, outlining the phylogenetic placement, metabolic features, host interactions, and unique genetic code usage of Minisyncoccota. Minisyncoccota play complementary and interdependent roles within microbial communities, while being restricted by incomplete metabolic capabilities that prevent independent survival. Studies on Minisyncoccota offer important insights into the diversity and evolution of uncultivated bacteria, as well as the hidden interaction networks that shape microbial ecosystems.</p>","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"41 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13293708/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147931157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Promoter Sequences Do Not Solely Govern nosZ Expression Differences between Bradyrhizobium ottawaense and B. diazoefficiens.","authors":"Sawa Wasai-Hara, Yoshikazu Shimoda, Hisayuki Mitsui, Shusei Sato, Haruko Imaizumi-Anraku, Kiwamu Minamisawa","doi":"10.1264/jsme2.ME25079","DOIUrl":"10.1264/jsme2.ME25079","url":null,"abstract":"<p><p>Nitrous oxide (N₂O) is a potent greenhouse gas, and the enzyme Nos catalyzes its reduction to dinitrogen (N₂). Bradyrhizobium ottawaense exhibits strong N₂O-reducing activity with high nosZ expression. To investigate whether promoter sequences affect nosZ expression, we constructed reciprocal promoter-swapped mutants between B. ottawaense and B. diazoefficiens. The swapping of promoters did not significantly affect expression levels. B. ottawaense mutants maintained approximately 200-fold higher expression levels than B. diazoefficiens, and the introduction of the B. ottawaense promoter into B. diazoefficiens did not increase expression levels. Therefore, the present results indicate that promoter sequence differences are not the primary factor affecting nosZ expression, suggesting regulation by other factors.</p>","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"41 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12999732/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147355663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Directional Colony Growth of Cupriavidus toward Sphingomonads.","authors":"Hiromi Kato, Shoko Hirano, Chiaki Haga, Sayaka Sakogawa, Yoshiyuki Ohtsubo, Yuji Nagata","doi":"10.1264/jsme2.ME25087","DOIUrl":"10.1264/jsme2.ME25087","url":null,"abstract":"<p><p>Bacterial communities often originate from physical encounters between distinct species on solid surfaces; however, the mechanisms underlying these initial interactions remain unclear. The γ-hexachlorocyclohexane (γ-HCH; a toxic recalcitrant insecticide)-degrading consortium includes the surface-motile bacterium Cupriavidus sp. strain TKC and the γ-HCH-degrading Sphingobium sp. strain TKS. During a co-culture on R2A agar, TKC colonies exhibited directional colony growth (DCG), characterized by asymmetric expansion toward neighboring TKS colonies. When TKC colonies eventually overgrew TKS colonies, non-motile TKS cells were passively carried along the expanding TKC front, indicating that DCG facilitates the physical association and surface dispersal of Sphingobium cells. DCG activity was also observed against various strains of Sphingobium, Sphingomonas, and Novosphingobium, whereas extremely weak or no DCG activity was observed toward other bacterial groups, including representatives of α-, β-, and γ-proteobacteria as well as actinobacteria. These results indicate that TKC exhibits DCG selectively toward sphingomonads, recognizing taxon-specific cues rather than responding indiscriminately to neighboring colonies. Among the components tested, sphingosine reproducibly triggered DCG in a dose-dependent manner, and the inhibition of sphingolipid biosynthesis in Sphingobium with myriocin markedly suppressed the inducing effect. These results highlight the sphingosine-mediated induction of DCG as a molecular mechanism underlying the initial step in the spatial organization of bacterial communities on solid surfaces.</p>","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"41 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13293705/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147775693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Symbiont- and Bacterial Cell Size-dependent Backpacking and Grazing of Acanthamoeba.","authors":"Nana Tanaka, Kotoka Kuriki, Torahiko Okubo, Yoshiaki Enoeda, Ayano Konno, Shinji Nakamura, Toyotaka Sato, Shin-Ichi Yokota, Hiroyuki Yamaguchi","doi":"10.1264/jsme2.ME25024","DOIUrl":"10.1264/jsme2.ME25024","url":null,"abstract":"<p><p>On solid media, Acanthamoeba harboring the endosymbiotic bacterium (Neochlamydia) carries live Escherichia coli on its cell surface without phagocytosing this bacterium, and defends against infection by Legionella pneumophila of a small size in an endosymbiotic bacterium-dependent manner. This implies the presence of an unknown protective mechanism. Therefore, we exami-ned the physical properties of the carried bacteria using transposon insertion mutants that had lost the \"backpack\" on solid media. A mutant was selected from a library in which the nhaA gene, encoding a Na⁺/H⁺ antiporter, was disrupted. The knockout mutant was longer than the parental strain and was gradually consumed by symbiotic amoebae. Similarly, the NhaA-knockout mutant strain was longer and lacked the backpack. The complementation of nhaA restored bacterial cells to their normal size, and the backpack phenomenon reappeared. Using E. coli elongated by a treatment with mitomycin C, the backpack was not evident, and enlarged bacteria were consumed by symbiotic amoebae. Therefore, symbiotic amoebae protected themselves from intruders by not engulfing small bacteria in an endosymbiosis-dependent manner. The present results propose a novel countermeasure by phagocytic cells against intruders that involves the recognition of bacterial sizes and is dependent on endosymbiosis.</p>","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"41 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12999725/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145917828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitrifying Communities in Biological Nitrogen Removal Processes at Tropical Municipal Wastewater Treatment Plants.","authors":"Liang Feng, Jia Xing Loi, Joana Séneca, Petra Pjevac, Faidzul Hakim Adnan, Gek Cheng Ngoh, Bee Chin Khor, Alijah Mohd Aris, Mamoru Oshiki, Holger Daims, Adeline Seak May Chua","doi":"10.1264/jsme2.ME25036","DOIUrl":"10.1264/jsme2.ME25036","url":null,"abstract":"<p><p>Nitrifying communities in activated sludge play a crucial role in biological nitrogen removal processes in municipal wastewater treatment plants. While extensive research has been conducted in temperate regions, limited information is available on nitrifiers in tropical regions. The present study investigated all currently known nitrifying communities in two full-scale municipal wastewater treatment plants in Malaysia operated under low-dissolved oxygen (DO) (0.2-0.7‍ ‍mg‍ ‍DO‍ ‍L^-1) or high-DO (2.0-5.5‍ ‍mg‍ ‍DO‍ ‍L^-1) conditions at 30°C. The core nitrifiers in the municipal wastewater treatment plants were Nitrosomonas (ammonia-oxidizing bacteria, AOB), Nitrospira (nitrite-oxidizing or complete ammonia-oxidizing, comammox, bacteria), and ammonia-oxidizing archaea (AOA) as identified by a 16S rRNA gene amplicon sequencing ana-lysis and corroborated by 16S rRNA-targeted fluorescence in situ hybridization. A quantitative polymerase chain reaction (qPCR) targeting ammonia monooxygenase subunit A (amoA) genes revealed stable populations of comammox Nitrospira and AOB in both wastewater treatment plants. AOA were detected in only one of the plants and their population sizes fluctuated, with higher temporary abundance under high-DO conditions. These results provide important insights into the composition and dynamics of nitrifying communities in tropical municipal wastewater treatment plants.</p>","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"40 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12501877/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145033697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimal Phosphate Concentration for Growth and Normal Functioning of Marine Anammox Bacteria, Candidatus Scalindua sp.","authors":"Thelwadanage Nadisha Tharangani Kumari Nawarathna, Haruhi Iida, Naoki Fujii, Noriatsu Ozaki, Akiyoshi Ohashi, Jonathan A C Roques, Tomonori Kindaichi","doi":"10.1264/jsme2.ME25042","DOIUrl":"10.1264/jsme2.ME25042","url":null,"abstract":"<p><p>The anammox process using marine anammox bacteria is a promising nitrogen removal process for recirculating aquaculture system wastewater. Marine anammox bacteria are typically found in oxygen-deficient zones and coastal areas under low phosphate concentrations. The optimal phosphate concentration for marine anammox bacteria remains unknown because most laboratory studies on these bacteria have been conducted under high phosphate concentrations. Therefore, the present study investigated the long-term effects of varying phosphate concentrations on the marine anammox bacteria, Candidatus Scalindua sp., to identify the optimal range of phosphate. Anammox activity and average growth rates were evaluated under seven phosphate concentrations (0, 0.23, 0.46, 0.68, 1.14, 6.15 [control], and 15.48‍ ‍mg P L^-1) over a period of 70 days. After 50 days of reactor operation, reactor performance under phosphate concentrations ranging from 0.23 to 6.15‍ ‍mg P L^-1 stabilized at 70% of total nitrogen removal efficiency, indicating the successful establishment of the anammox process. Conversely, anammox reactor performance under conditions without phosphate addition (0‍ ‍mg P L^-1) and the highest phosphate concentration (15.48‍ ‍mg P L^-1) did not reach 70% of total nitrogen removal efficiency, indicating a suboptimal phosphate concentration for normal anammox activity. Average growth rates calculated from total biomass samples varied from 0.0006 to 0.0012 h^-1. These results indicate that Ca. Scalindua need to be kept at phosphate concentrations between 0.23 and 6.15‍ ‍mg P L^-1 for optimal functioning in wastewater treatment ecosystems.</p>","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"40 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12727191/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145505397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ammonia Oxidation Property of Soils on the Glacier Foreland of Austre Brøggerbreen, Svalbard: Response to Open-top Chamber Experiments.","authors":"Kentaro Hayashi, Keisuke Ono, Yukiko Tanabe, Masahito Hayatsu, Kanako Tago, Tsubasa Ohbayashi, Yong Wang, Luciano Nobuhiro Aoyagi, Masaki Uchida","doi":"10.1264/jsme2.ME25058","DOIUrl":"10.1264/jsme2.ME25058","url":null,"abstract":"<p><p>Recent warming and glacier retreat in the Svalbard Archipelago, part of the Arctic cryosphere, have become increasingly evident. The present study investigated the foreland of Austre Brøggerbreen near Ny-Ålesund to clarify how soil nitrification responds to changing conditions. Two sites, exposed for different periods following glacier retreat, were compared. A manipulation experiment using open-top chambers (OTCs) and homogenized initial soil conditions was conducted to assess the effects of site differences, the OTC treatment, soil depth, and interannual variations on soil and nitrification properties. Although the OTC treatment slightly increased soil temperature and moisture, its overall effect on soil properties, ammonia oxidation potential (AOP), and microbial properties was negligible. In contrast, homogenization markedly increased total nitrogen at both sites and temporarily boosted AOPs for two years before levels returned to baseline. Site 2, exposed for longer, contained more soil nitrogen and showed higher AOP than Site 1. For example, in 2015, AOPs at 10°C were 3.5 and 2.4‍ ‍ng N g^-1 dry soil h^-1 at Sites 2 and 1, respectively. Ammonia-oxidizing bacteria (AOB) and archaea (AOA) were both more abundant at Site 2, although AOB clearly dominated at both sites. While AOB-amoA operational taxonomic units (OTUs) were mostly shared between sites, community compositions differed: OTU2 was prevalent at Site 1, but minor at Site 2. OTU2 may act as a pioneer taxon that declines in later stages, or its pattern may reflect site-specific soil conditions. A phylogenetic anal-ysis showed that OTU2 and OTU3 belonged to Cluster ME found near Mount Everest.</p>","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"40 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12727203/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145557208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Diversity of Endospore-forming Nitrogen-fixing Bacteria and Their Future Application as Biofertilizers in the Central Dry Zone of Myanmar.","authors":"Ya Maon Phoo, Koki Toyota, Yu Yu Min","doi":"10.1264/jsme2.ME25033","DOIUrl":"10.1264/jsme2.ME25033","url":null,"abstract":"<p><p>In Myanmar, the application of both nitrogen-based chemical fertilizers and biofertilizers is limited and this low input has caused poor agricultural yields. The present study aimed to isolate indigenous endospore-forming nitrogen-fixing bacteria (EFNFB) and examine their potential for co-inoculation with agricultural waste. A total of 387 isolates were obtained from 42 different soil samples in the central dry zone of Myanmar using nitrogen-free Rennie medium. Nitrogen-fixing activity (NFA) assessed with the acetylene reduction assay was positive in 102 isolates. A phylogenetic ana-lysis based on 16S rRNA sequences identified 25 different species, including the genera Paenibacillus, Priestia, Bacillus, Brevibacillus, Sporolactobacillus, Niallia, and Neobacillus. Among these genera, Paenibacillus spp. was the predominant genus, comprising 51 isolates (64%) across 16 different species (64%) that were prevalent in soils rotated with rice and pulses. Paenibacillus spp. showed different NFA levels in Rennie medium. Eleven species belonging to different genera had not been previously documented as nitrogen-fixing bacteria. NFA levels were evaluated in soil inoculated with EFNFB and rice straw or mung bean residue. The results obtained demonstrated that NFA levels were dependent on isolates and the type of agricultural waste. NFA in soil was significantly increased by inoculations with some isolates, suggesting their potential as biofertilizers. The inoculation of Priestia aryabhattai S10 with rice straw or mung bean resulted in significantly higher NFA levels in soil. These results indicate the potential of EFNFB as biofertilizer inoculants in Myanmar.</p>","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"40 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12727198/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145756999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-pressure Methanogenesis Reveals Metabolic Adaptation to Dissolved CO₂ Limitation.","authors":"Taiki Katayama, Hideyoshi Yoshioka, Masaru K Nobu","doi":"10.1264/jsme2.ME25066","DOIUrl":"10.1264/jsme2.ME25066","url":null,"abstract":"<p><p>This study investigated the effects of elevated hydrostatic pressure on methane production and gene expression in a hydrogenotrophic methanogen isolated from subseafloor sediments at biogenic gas hydrate sites, with a focus on the implications of CO₂ availability. Using high-pressure cultivation, the methane production rate decreased by 15% at 25 MPa, while a transcriptomic anal-ysis revealed the marked up-regulation of methyl-coenzyme M reductase and ATP synthase. These results suggest that methanogens compensate for pressure-driven constraints on CO₂ utilization by increasing the expression of key methanogenic enzymes, underscoring the overlooked role of CO₂ in deep biosphere microbial processes.</p>","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"40 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12727201/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145757038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fusarium Fungi Produce Nitrous Oxide (N₂O) from Nitrite (NO₂^-) in a Model Pot System Simulating the Soybean Rhizosphere.","authors":"Makoto Moriuchi, Keiichi Kuzunuki, Fumio Ikenishi, Reiko Sameshima, Akira Nakagiri, Sakae Toyoda, Chie Katsuyama, Kaori Kakizaki, Manabu Itakura, Naohiro Yoshida, Yuichi Suwa, Kiwamu Minamisawa","doi":"10.1264/jsme2.ME24092","DOIUrl":"10.1264/jsme2.ME24092","url":null,"abstract":"<p><p>Nitrous oxide (N₂O) is a key atmospheric greenhouse gas that contributes to global warming, with anthropogenic N₂O emissions from agriculture being a particular concern. Among agricultural sources, unknown soil organisms in the legume rhizosphere emit N₂O from degraded root nodules. To discriminate between fungal and bacterial N₂O emissions, we adopted an isotopomer ana-lysis, which provides site preference values (the difference in ¹⁵N abundance of the central and terminal N atoms in the N₂O molecule). The addition of nitrite instead of nitrate to soybean nodulated roots significantly increased SP_N2O from -3.5‰ to 4.2‰ in a pot system. Moreover, a mutation of the nirK gene (encoding dissimilatory nitrite reductase) in symbiotic bradyrhizobia significantly increased SP_N2O from 4.2‰ to 13.9‰ with nitrite. These results suggest that nitrite-utilizing N₂O emissions via fungal denitrification occurred in the model pot system of the soybean rhizosphere. Microscopic observations showed fungal hyphae and crescent spores around N₂O-emitting nodules. Therefore, we isolated single spores from soybean nodules under a microscope. A phylogenetic ana-lysis revealed that all 12 fungal isolates were Fusarium species, which exist in soybean field soil. When these isolates were cultivated in glycerol-peptone medium supplemented with nitrate or nitrite (1‍ ‍mM), 11 of the 12 isolates strongly converted nitrite to N₂O; however, no N₂O emissions were noted in the presence of nitrate. A ¹⁵N-nitrite tracer experiment revealed that one N₂O molecule was derived exclusively from two molecules of nitrite (NO₂^-) in the fungal culture. These results suggest that nitrite-utilizing Fusarium fungi mediate N₂O emissions in the soybean rhizosphere.</p>","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"40 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12213065/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}