Microbes and Environments最新文献_第10页

Detection and Characterization of RNA Viruses in Red Macroalgae (Bangiaceae) and Their Food Product (Nori Sheets). 红色大型藻类（Bangiaceae）及其食品（紫菜片）中 RNA 病毒的检测和特征描述。

IF 2.1 4区环境科学与生态学

Microbes and Environments Pub Date : 2022-01-01 DOI: 10.1264/jsme2.ME21084

Yukino Mizutani, Yuto Chiba, Syun-Ichi Urayama, Yuji Tomaru, Daisuke Hagiwara, Kei Kimura

{"title":"Detection and Characterization of RNA Viruses in Red Macroalgae (Bangiaceae) and Their Food Product (Nori Sheets).","authors":"Yukino Mizutani, Yuto Chiba, Syun-Ichi Urayama, Yuji Tomaru, Daisuke Hagiwara, Kei Kimura","doi":"10.1264/jsme2.ME21084","DOIUrl":"10.1264/jsme2.ME21084","url":null,"abstract":"Persistent RNA viruses, which have been suggested to form symbiotic relationships with their hosts, have been reported to occur in eukaryotes, such as plants, fungi, and algae. Based on empirical findings, these viruses may also be present in commercially cultivated macroalgae. Accordingly, the present study aimed to screen red macroalgae (family Bangiaceae conchocelis and Neopyropia yezoensis thallus) and processed nori sheets (N. yezoensis) for persistent RNA viruses using fragmented and primer-ligated dsRNA sequencing (FLDS) and targeted reverse transcription PCR (RT-PCR). A Totiviridae-related virus was detected in the conchocelis of Neoporphyra haitanensis, which is widely cultivated in China, while two Mitoviridae-related viruses were found in several conchocelis samples and all N. yezoensis-derived samples (thallus and nori sheets). Mitoviridae-related viruses in N. yezoensis are widespread among cultivated species and not expected to inhibit host growth. Mitoviridae-related viruses were also detected in several phylogenetically distant species in the family Bangiaceae, which suggests that these viruses persisted and coexist in the family Bangiaceae over a long period of time. The present study is the first to report persistent RNA viruses in nori sheets and their raw materials.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"37 5","pages":""},"PeriodicalIF":2.1,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9763034/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10838138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Putative Invertebrate, Plant, and Wastewater Derived ssRNA Viruses in Plankton of the Anthropogenically Impacted Anacostia River, District of Columbia, USA. 美国哥伦比亚特区阿纳科斯蒂亚河浮游生物中推测的无脊椎动物、植物和废水衍生的ssRNA病毒。

IF 2.2 4区环境科学与生态学

Microbes and Environments Pub Date : 2022-01-01 DOI: 10.1264/jsme2.ME21070

Caroline Solomon, Ian Hewson

{"title":"Putative Invertebrate, Plant, and Wastewater Derived ssRNA Viruses in Plankton of the Anthropogenically Impacted Anacostia River, District of Columbia, USA.","authors":"Caroline Solomon, Ian Hewson","doi":"10.1264/jsme2.ME21070","DOIUrl":"https://doi.org/10.1264/jsme2.ME21070","url":null,"abstract":"The Anacostia River is a highly impacted watershed in the Northeastern United States which experiences combined sewage outfall in downstream waters. We examined the composition of RNA viruses at three sites in the river using viral metagenomics. Viromes had well represented Picornaviruses, Tombusviruses, Wolframviruses, Nodaviruses, with fewer Tobamoviruses, Sobemoviruses, and Densoviruses (ssDNA). Phylogenetic ana-lyses of detected viruses provide evidence for putatively autochthonous and allochthonous invertebrate, plant, and vertebrate host origin. The number of viral genomes matching Ribovaria increased downstream, and assemblages were most disparate between distant sites, suggesting impacts of the combined sewage overflows at these sites. Additionally, we recovered a densovirus genome fragment which was highly similar to the Clinch ambidensovirus 1, which has been attributed to mass mortality of freshwater mussels in Northeastern America. Taken together, these data suggest that RNA viromes of the Anacostia River reflect autochthonous production of virus particles by benthic metazoan and plants, and inputs from terrestrial habitats including sewage.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"37 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9763036/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10470086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Degenerate PCR Targeting the Major Capsid Protein Gene of HcRNAV and Related Viruses. 针对HcRNAV及相关病毒衣壳蛋白主要基因的退化PCR。

IF 2.2 4区环境科学与生态学

Microbes and Environments Pub Date : 2022-01-01 DOI: 10.1264/jsme2.ME21075

Michiko Takahashi, Kei Wada, Syun-Ichi Urayama, Yuichi Masuda, Keizo Nagasaki

引用次数: 1

Delayed Lysis Time at High Multiplicities of Particles in a Chlorovirus-Chlorella Interaction. 绿病毒-小球藻相互作用中高倍数粒子的延迟裂解时间。

IF 2.2 4区环境科学与生态学

Microbes and Environments Pub Date : 2022-01-01 DOI: 10.1264/jsme2.ME22068

Eva J P Lievens, Manuela Spagnuolo, Tom Réveillon, Lutz Becks

引用次数: 3

Supplemental Clostridium butyricum MIYAIRI 588 Affects Intestinal Bacterial Composition of Finishing Pigs. 添加丁酸梭菌MIYAIRI 588对育肥猪肠道细菌组成的影响

IF 2.2 4区环境科学与生态学

Microbes and Environments Pub Date : 2022-01-01 DOI: 10.1264/jsme2.ME22011

Maki Hirata, Miki Matsuoka, Takuma Hashimoto, Takamichi Oura, Yo Ohnuki, Chika Yoshida, Ayaka Minemura, Daiki Miura, Kentaro Oka, Motomichi Takahashi, Fumiki Morimatsu

{"title":"Supplemental Clostridium butyricum MIYAIRI 588 Affects Intestinal Bacterial Composition of Finishing Pigs.","authors":"Maki Hirata, Miki Matsuoka, Takuma Hashimoto, Takamichi Oura, Yo Ohnuki, Chika Yoshida, Ayaka Minemura, Daiki Miura, Kentaro Oka, Motomichi Takahashi, Fumiki Morimatsu","doi":"10.1264/jsme2.ME22011","DOIUrl":"https://doi.org/10.1264/jsme2.ME22011","url":null,"abstract":"Animal gastrointestinal tracts are populated by highly diverse and complex microbiotas. The gut microbiota influences the bioavailability of dietary components and is closely associated with physiological processes in the host. Clostridium butyricum reportedly improves growth performance and affects the gut microbiota and immune functions in post-weaning piglets. However, the effects of C. butyricum on finishing pigs remain unclear. Therefore, we herein investigated the effects of C. butyricum MIYAIRI 588 (CBM588) on the gut microbiota of finishing pigs. 16S rRNA gene sequencing was performed using fecal samples and ileal, cecal, and colonic contents collected after slaughtering. The α-diversity of the small intestinal microbiota was lower than that of the large intestinal microbiota, whereas β-diversity showed different patterns depending on sample collection sites. The administration of CBM588 did not significantly affect the α- or β-diversity of the microbiotas of fecal and intestinal content samples regardless of the collection site. However, a linear discriminant ana-lysis Effect Size revealed that the relative abundance of Lactobacillaceae at the family level, Bifidobacterium at the order level, and Lactobacillus ruminis and Bifidobacterium pseudolongum at the species level were higher in the fecal samples and cecal and colonic contents of the treatment group than in those of the control group. Therefore, the administration of CBM588 to finishing pigs affected the composition of the gut microbiota and increased the abundance of bacteria that are beneficial to the host. These results provide important insights into the effects of probiotic administration on relatively stable gut microbial ecosystems.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"37 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9530721/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33494913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Single Shot of Vesicles. 单针囊泡。

IF 2.2 4区环境科学与生态学

Microbes and Environments Pub Date : 2022-01-01 DOI: 10.1264/jsme2.ME22083

Masanori Toyofuku, Yousuke Kikuchi, Azuma Taoka

{"title":"A Single Shot of Vesicles.","authors":"Masanori Toyofuku, Yousuke Kikuchi, Azuma Taoka","doi":"10.1264/jsme2.ME22083","DOIUrl":"https://doi.org/10.1264/jsme2.ME22083","url":null,"abstract":"Bacteria communicate through signaling molecules that coordinate group behavior. Hydrophobic signals that do not diffuse in aqueous environments are used as signaling molecules by several bacteria. However, limited information is currently available on the mechanisms by which these molecules are transported between cells. Membrane vesicles (MVs) with diverse functions play important roles in the release and delivery of hydrophobic signaling molecules, leading to differences in the dynamics of signal transportation from those of free diffusion. Studies on Paracoccus denitrificans, which produces a hydrophobic long-chain N-acyl homoserine lactone (AHL), showed that signals were loaded into MVs at a concentration with the potential to trigger the quorum sensing (QS) response with a \"single shot\" to the cell. Furthermore, stimulating the formation of MVs increased the release of signals from the cell; therefore, a basic understanding of MV formation is important. Novel findings revealed the formation of MVs through different routes, resulting in the production of different types of MVs. Methods such as high-speed atomic force microscopy (AFM) phase imaging allow the physical properties of MVs to be analyzed at a nanometer resolution, revealing their heterogeneity. In this special minireview, we introduce the role of MVs in bacterial communication and highlight recent findings on MV formation and their physical heterogeneity by referring to our research. We hope that this minireview will provide basic information for understanding the functionality of MVs in ecological systems.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"37 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10037094/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9184073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RNA Virosphere in a Marine Zooplankton Community in the Subtropical Western North Pacific. 北太平洋西部亚热带海洋浮游动物群落的RNA病毒圈。

IF 2.2 4区环境科学与生态学

Microbes and Environments Pub Date : 2022-01-01 DOI: 10.1264/jsme2.ME21066

Junya Hirai, Syun-Ichi Urayama, Yoshiro Takaki, Miho Hirai, Keizo Nagasaki, Takuro Nunoura

{"title":"RNA Virosphere in a Marine Zooplankton Community in the Subtropical Western North Pacific.","authors":"Junya Hirai, Syun-Ichi Urayama, Yoshiro Takaki, Miho Hirai, Keizo Nagasaki, Takuro Nunoura","doi":"10.1264/jsme2.ME21066","DOIUrl":"https://doi.org/10.1264/jsme2.ME21066","url":null,"abstract":"Zooplankton and viruses play a key role in marine ecosystems; however, their interactions have not been examined in detail. In the present study, the diversity of viruses associated with zooplankton collected using a plankton net (mesh size: 100‍ ‍μm) in the subtropical western North Pacific was investigated by fragmented and primer ligated dsRNA sequencing. We obtained 21 and 168 operational taxonomic units (OTUs) of ssRNA and dsRNA viruses, respectively, containing RNA-dependent RNA polymerase (RdRp). These OTUs presented average amino acid similarities of 43.5 and 44.0% to the RdRp genes of known viruses in ssRNA viruses and dsRNA viruses, respectively. Dominant OTUs mainly belonged to narna-like and picorna-like ssRNA viruses and chryso-like, partiti-like, picobirna-like, reo-like, and toti-like dsRNA viruses. Phylogenetic ana-lyses of the RdRp gene revealed that OTUs were phylogenetically diverse and clustered into distinct clades from known viral groups. The community structure of the same zooplankton sample was investigated using small subunit (SSU) rRNA sequences assembled from the metatranscriptome of single-stranded RNA. More than 90% of the sequence reads were derived from metazoan zooplankton; copepods comprised approximately 70% of the sequence reads. Although this ana-lysis provided no direct evidence of the host species of RNA viruses, these dominant zooplankton are expected to be associated with the RNA viruses detected in the present study. The present results indicate that zooplankton function as a reservoir of diverse RNA viruses and suggest that investigations of zooplankton viruses will provide a more detailed understanding of the role of viruses in marine ecosystems.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"37 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9763039/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10528098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Evaluation of Disease Suppressiveness of Soils in Croplands by Co-Cultivation of Pathogenic Fusarium oxysporum and Indigenous Soil Microorganisms. 病原菌尖孢镰刀菌与土生微生物共同栽培对农田土壤抗病能力的评价。

IF 2.2 4区环境科学与生态学

Microbes and Environments Pub Date : 2022-01-01 DOI: 10.1264/jsme2.ME21063

Masahiro Mitsuboshi, Yuuzou Kioka, Katsunori Noguchi, Susumu Asakawa

{"title":"Evaluation of Disease Suppressiveness of Soils in Croplands by Co-Cultivation of Pathogenic Fusarium oxysporum and Indigenous Soil Microorganisms.","authors":"Masahiro Mitsuboshi, Yuuzou Kioka, Katsunori Noguchi, Susumu Asakawa","doi":"10.1264/jsme2.ME21063","DOIUrl":"https://doi.org/10.1264/jsme2.ME21063","url":null,"abstract":"An evaluation of suppressiveness against soil-borne diseases is important for their control. We herein assessed disease suppression against F. oxysporum f. sp. spinaciae using the Fusarium co-cultivation method in 75 soils collected from croplands around the country. The suppressive effects of soil microbes against F. oxysporum growth were examined by simultaneously culturing soil suspensions and F. oxysporum f. sp. spinaciae on a culture medium. The growth degree of F. oxysporum on the medium varied among the 75 soils tested, and 14 soils showing different degrees of growth were selected to investigate the incidence of spinach wilt by cultivating spinach inoculated with the pathogenic F. oxysporum strain. A correlation (r=0.831, P<0.001) was observed between the disease incidence of spinach wilt and the growth degree of F. oxysporum using the co-cultivation method in the 14 selected soils. No correlations were noted between chemical and biological parameters (including pH and the population density of microbes, except for the growth degree of F. oxysporum) and the growth degree of F. oxysporum and incidence of spinach wilt, indicating that it was not possible to predict the degree of growth or disease incidence based on specific chemical and biological characteristics or their combination. The present results suggest that an evaluation of the growth degree of F. oxysporum by the Fusarium co-cultivation will be useful for diagnosing the disease suppressiveness of soil against pathogenic F. oxysporum in croplands.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"37 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9763048/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10823125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Occurrence and Diversity of Listeria monocytogenes Isolated from Two Pig Manure Treatment Plants in France. 法国两个猪粪处理厂分离的单核细胞增生李斯特菌的发生和多样性。

IF 2.2 4区环境科学与生态学

Microbes and Environments Pub Date : 2022-01-01 DOI: 10.1264/jsme2.ME22019

Martine Denis, Christine Ziebal, Evelyne Boscher, Sylvie Picard, Morgane Perrot, Meryl Vila Nova, Sophie Roussel, Arnaud Diara, Anne-Marie Pourcher

{"title":"Occurrence and Diversity of Listeria monocytogenes Isolated from Two Pig Manure Treatment Plants in France.","authors":"Martine Denis, Christine Ziebal, Evelyne Boscher, Sylvie Picard, Morgane Perrot, Meryl Vila Nova, Sophie Roussel, Arnaud Diara, Anne-Marie Pourcher","doi":"10.1264/jsme2.ME22019","DOIUrl":"https://doi.org/10.1264/jsme2.ME22019","url":null,"abstract":"The presence of Listeria monocytogenes in piggery effluents intended for irrigation crops may be a source of bacterial dissemination in agriculture. The occurrence and diversity of L. monocytogenes in the farm environment were examined in two pig manure treatment systems (S1 and S2). Samples collected over the course of one year consisted of manure, the liquid fraction of treated manure (lagoon effluent), and soil surrounding the lagoon. L. monocytogenes was enumerated using the Most Probable Number (MPN) method, serotyped by PCR, genotyped by pulsed-field gel electrophoresis (PFGE), and sequenced for multilocus sequence typing (MLST). L. monocytogenes was detected in 92% of manure samples and in approximately 50% of lagoon effluent and soil samples. Concentrations ranged between 5 and 103 MPN 100‍ ‍mL-1. Serogroups IIa, IIb, and IVb were identified. Diversity was high with 44 PFGE profiles (252 isolates) and 17 clonal complexes (CCs) (96 isolates) with higher diversity in manure at site S1 supplied by four farms. Some PFGE profiles and CCs identified in manure or in pig feces from a previous study were also detected in lagoons and/or soil, reflecting pig L. monocytogenes circulation throughout the manure treatment and in the vicinity of the sampling sites. However, some PFGE profiles and CCs were only found in the lagoon and/or in soil, suggesting an origin other than pigs. The present study highlights the limited ability of biological treatments to eliminate L. monocytogenes from pig manure. The persistence of some PFGE profiles and CCs throughout the year in the lagoon and soil shows the ability of L. monocytogenes to survive in this type of environment.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"37 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9763045/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10474211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Mitigation of Paddy Field Soil Methane Emissions by Betaproteobacterium Azoarcus Inoculation of Rice Seeds. 偶氮变形杆菌接种水稻种子减少稻田土壤甲烷排放

IF 2.2 4区环境科学与生态学

Microbes and Environments Pub Date : 2022-01-01 DOI: 10.1264/jsme2.ME22052

Midori Sakoda, Takeshi Tokida, Yoriko Sakai, Keishi Senoo, Tomoyasu Nishizawa

{"title":"Mitigation of Paddy Field Soil Methane Emissions by Betaproteobacterium Azoarcus Inoculation of Rice Seeds.","authors":"Midori Sakoda, Takeshi Tokida, Yoriko Sakai, Keishi Senoo, Tomoyasu Nishizawa","doi":"10.1264/jsme2.ME22052","DOIUrl":"https://doi.org/10.1264/jsme2.ME22052","url":null,"abstract":"Paddy fields are a major source of atmospheric methane, a greenhouse gas produced by methanogens and consumed by methanotrophs in flooded soil. The inoculation of rice seeds with the bacterium Azoarcus sp. KH32C alters the rice root-associated soil bacterial community composition. The present study investigated the effects of KH32C-inoculated rice cultivation on soil methanogens and methanotrophs involved in methane emissions from a rice paddy field. KH32C-inoculated and non-inoculated rice (cv. Nipponbare) were cultivated in a Japanese rice paddy with and without nitrogen fertilizer. Measurements of methane emissions and soil solution chemical properties revealed increases in methane flux over the waterlogged period with elevations in the concentrations of dissolved methane, dissolved organic carbon, and ferrous iron, which is an indicator of soil reduction levels. Reverse transcription quantitative PCR and amplicon sequencing were used to assess the transcription of the methyl-coenzyme M reductase gene (mcrA) from methanogens and the particulate methane monooxygenase gene (pmoA) from methanotrophs in paddy soil. The results obtained showed not only the transcript copy numbers, but also the compositions of mcrA and pmoA transcripts were related to methane flux. KH32C-inoculated rice cultivation recruited soil methanogens and methanotrophs that suppressed high methane synthesis, increased methane consumption, and decreased methane emissions by 23.5 and 17.2% under non-fertilized and nitrogen-fertilized conditions, respectively, while maintaining rice grain yield. The present study demonstrated the mitigation of paddy field methane emissions arising from the use of KH32C in rice cultivation due to its influence on the compositions of soil methanogen and methanotroph populations.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"37 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9763044/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10825229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0