Microbes and Environments最新文献_第9页

Symbiosis Contribution of Non-nodulating Bradyrhizobium cosmicum S23321 after Transferal of the Symbiotic Plasmid pDOA9 转移共生质粒pDOA9后非结瘤慢生根瘤菌粘粒S23321的共生贡献

IF 2.2 4区环境科学与生态学

Microbes and Environments Pub Date : 2022-06-08 DOI: 10.1264/jsme2.ME22008

D. Wulandari, P. Tittabutr, Pongpan Songwattana, P. Piromyou, Kamonluck Teamtisong, N. Boonkerd, Pakpoom Boonchuen, N. Teaumroong

{"title":"Symbiosis Contribution of Non-nodulating Bradyrhizobium cosmicum S23321 after Transferal of the Symbiotic Plasmid pDOA9","authors":"D. Wulandari, P. Tittabutr, Pongpan Songwattana, P. Piromyou, Kamonluck Teamtisong, N. Boonkerd, Pakpoom Boonchuen, N. Teaumroong","doi":"10.1264/jsme2.ME22008","DOIUrl":"https://doi.org/10.1264/jsme2.ME22008","url":null,"abstract":"The symbiotic properties of rhizobial bacteria are driven by the horizontal gene transfer of symbiotic genes, which are located in symbiosis islands or on plasmids. The symbiotic megaplasmid pDOA9 of Bradyrhizobium sp. DOA9, carrying the nod, nif, fix, and type three secretion system (T3SS) genes, has been conjugatively transferred to different Bradyrhizobium strains. In the present study, non-nodulating B. cosmicum S23321, which shows a close phylogenetic relationship with Bradyrhizobium sp. DOA9, but lacks symbiotic properties, was used to carry pDOA9 (annotated as chimeric S2:pDOA9). The results obtained showed that pDOA9 conferred symbiotic properties on S23321; however, nodulation phenotypes varied among the DOA9, chimeric ORS278:pDOA9, and S2:pDOA9 strains even though they all carried symbiotic pDOA9 plasmid. S23321 appeared to gain symbiotic nodulation from pDOA9 by processing nodulation genes and broadening the host range. The present results also showed the successful formation of active nodules in Arachis hypogaea (Dalbergoid) and Vigna radiata (Millitoid) by chimeric S2:pDOA9, while Crotalaria juncea (Genistoid) and Macroptilium atropurpureum (Millitoid) formed nodule-like structures. The formation of nodules and nodule-like structures occurred in a nod factor-dependent manner because the nod factor-lacking strain (S2:pDOA9ΩnodB) completely abolished nodulation in all legumes tested. Moreover, T3SS carried by S2:pDOA9 exerted negative effects on symbiosis with Crotalaria juncea, which was consistent with the results obtained on DOA9. T3SS exhibited symbiotic compatibility with V. radiata when nodulated by S23321. These outcomes implied that pDOA9 underwent changes during legume evolution that broadened host specificity and the compatibility of nodulation in a manner that was dependent on the chromosomal background of the recipient as well as legume host restrictions.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47639697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Weekly Observations of Estuarine Microbial Assemblages during Summer in the Inner Part of Ariake Bay, Japan; Microbial Water-sediment Coupling in Turbid Shallow Waters 日本有明湾中部夏季河口微生物群落的周观测浑浊浅水微生物水-沉积物耦合研究

IF 2.2 4区环境科学与生态学

Microbes and Environments Pub Date : 2022-06-08 DOI: 10.1264/jsme2.ME22015

R. Orita, K. Yoshida, Hiroto Terazono, Y. Nagano, M. Goto, K. Kimura, Genta Kobayashi

{"title":"Weekly Observations of Estuarine Microbial Assemblages during Summer in the Inner Part of Ariake Bay, Japan; Microbial Water-sediment Coupling in Turbid Shallow Waters","authors":"R. Orita, K. Yoshida, Hiroto Terazono, Y. Nagano, M. Goto, K. Kimura, Genta Kobayashi","doi":"10.1264/jsme2.ME22015","DOIUrl":"https://doi.org/10.1264/jsme2.ME22015","url":null,"abstract":"Estuarine microbial assemblages are altered by a number of environmental factors, and knowledge of these changes is essential for understanding the functions of microbes in estuarine ecosystems. The aims of the present study were to examine the relationship between microbial assemblages in the water column and sediment surface, and to identify the environmental factors that influence the short-term dynamics of microbial assemblages in these two zones in summer in the inner part of Ariake Bay. The microbial assemblage of each sample consisted of a mean of 71.1% operational taxonomic units (OTUs), which commonly occurred in the water column and sediment surface, although their relative composition markedly differed between the two zones. In the water column, spatiotemporal changes in microbial assemblages correlated with several environmental factors, such as the nitrogen content in suspended particles, turbidity, and salinity. On the other hand, temporal changes in the sediment’s microbial assemblages were governed by a single environmental factor, namely, the oxygen reduction potential. These results suggest that the composition of microbial assemblages in the water column and sediment surface differed even in highly turbid brackish waters with high sediment resuspension, and the environmental factors contributing to the change in the assemblage composition also differed between the water column and sediment.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44241460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity of Candidatus Patescibacteria in Activated Sludge Revealed by a Size-Fractionation Approach 用粒度分级法揭示活性污泥中念珠菌的多样性

IF 2.2 4区环境科学与生态学

Microbes and Environments Pub Date : 2022-06-08 DOI: 10.1264/jsme2.me22027

Shuka Kagemasa, Kyohei Kuroda, R. Nakai, Y. Li, K. Kubota

{"title":"Diversity of Candidatus Patescibacteria in Activated Sludge Revealed by a Size-Fractionation Approach","authors":"Shuka Kagemasa, Kyohei Kuroda, R. Nakai, Y. Li, K. Kubota","doi":"10.1264/jsme2.me22027","DOIUrl":"https://doi.org/10.1264/jsme2.me22027","url":null,"abstract":"Uncultivated members of Candidatus Patescibacteria are commonly found in activated sludge treating sewage and are widely distributed in wastewater treatment plants in different regions and countries. However, the phylogenetic diversity of Ca. Patescibacteria is difficult to examine because of their low relative abundance in the environment. Since Ca. Patescibacteria members have small cell sizes, we herein collected small microorganisms from activated sludge using a filtration-based size-fractionation approach (i.e., 0.45–0.22 μm and 0.22–0.1 μm fractions). Fractionated samples were characterized using 16S rRNA gene amplicon and shotgun metagenomic sequence analyses. The amplicon analysis revealed that the relative abundance of Ca. Patescibacteria increased to 73.5% and 52.5% in the 0.45–0.22 μm and 0.22–0.1 μm fraction samples, respectively, from 5.8% in the unfractionated sample. The members recovered from the two size-fractionated samples included Ca. Saccharimonadia, Ca. Gracilibacteria, Ca. Paceibacteria, Ca. Microgenomatia, class-level uncultured lineage ABY1, Ca. Berkelbacteria, WS6 (Ca. Dojkabacteria), and WWE3, with Ca. Saccharimonadia being predominant in both fraction samples. The number of operational taxonomic units belonging to Ca. Patescibacteria was approximately 6-fold higher in the size-fractionated samples than in the unfractionated sample. The shotgun metagenomic analysis of the 0.45–0.22 μm fractioned sample enabled the reconstruction of 24 high-quality patescibacterial bins. The bins obtained were classified into diverse clades at the family and genus levels, some of which were rarely detected in previous activated sludge studies. Collectively, the present results suggest that the overall diversity of Ca. Patescibacteria inhabiting activated sludge is higher than previously expected.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44441858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Effects of Different Types of Additional Fertilizers on Root-associated Microbes of Napa Cabbage Grown in an Andosol Field in Japan 不同施肥类型对日本安多醇田Napa甘蓝根系相关微生物的影响

IF 2.2 4区环境科学与生态学

Microbes and Environments Pub Date : 2022-05-31 DOI: 10.1264/jsme2.ME22013

S. Ikeda, K. Okazaki, Hirohito Tsurumaru, Takanori Suzuki, M. Hirafuji

引用次数: 0

A Minority Population of Non-dye-decolorizing Bacillus subtilis enhances the Azo Dye-decolorizing Activity of Enterococcus faecalis 少数非染料脱色枯草芽孢杆菌提高粪肠球菌偶氮染料脱色活性

IF 2.2 4区环境科学与生态学

Microbes and Environments Pub Date : 2022-05-31 DOI: 10.1264/jsme2.ME21080

Yuta Yamanashi, Tsukasa Ito

{"title":"A Minority Population of Non-dye-decolorizing Bacillus subtilis enhances the Azo Dye-decolorizing Activity of Enterococcus faecalis","authors":"Yuta Yamanashi, Tsukasa Ito","doi":"10.1264/jsme2.ME21080","DOIUrl":"https://doi.org/10.1264/jsme2.ME21080","url":null,"abstract":"Microbes live in communities in biological wastewater treatment plants and in the intestines. However, limited information is currently available on the mechanisms by which minority bacterial populations assist other bacteria besides syntrophic relationships as well as on the microbial food web. Therefore, the present study investigated the effects of non-dye-decolorizing Bacillus subtilis strain S4ga at population levels ranging between 0.04 and 4% on the activity of dye-decolorizing Enterococcus faecalis strain T6a1 using a dye decolorization assay. The results obtained revealed that the minority population of B. subtilis S4ga enhanced the dye-decolorizing activity of E. faecalis T6a1, resulting in a shorter lag time and longer active time of dye decolorization. These effects were related to redox potential values rather than O2 concentrations. Comparisons of the extracellular metabolites in individual incubations of E. faecalis T6a1 and B. subtilis S4ga and a co-incubation suggested a mutual relationship through the cross-feeding of specific amino acids (tyrosine, methionine, tryptophan, phenylalanine, valine, and leucine from B. subtilis S4ga to E. faecalis T6a1; glutamine, histidine, aspartic acid, and proline from E. faecalis T6a1 to B. subtilis S4ga). An analysis of intracellular primary metabolites indicated that the arginine deiminase (ADI) pathway, an ATP-producing energy-generating process, was more strongly activated in co-incubated E. faecalis T6a1 than in E. faecalis T6a1 incubated alone. These results suggest that a co-incubation with B. subtilis S4ga promoted ATP production by E. faecalis T6a1 cells and enhanced its dye-decolorizing activity.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44778182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection and Identification of Novel Intracellular Bacteria Hosted in Strains CBS 648.67 and CFCC 80795 of Biocontrol Fungi Metarhizium 绿僵菌生防菌CBS 648.67和CFCC 80795胞内新型细菌的检测与鉴定

IF 2.2 4区环境科学与生态学

Microbes and Environments Pub Date : 2022-05-24 DOI: 10.1264/jsme2.ME21059

Yue Ying, Chenglin Liu, Ran He, Ruizhen Wang, Liangjian Qu

引用次数: 0

Isolation and Characterization of Phosphate Solubilizing Bacteria from Paddy Field Soils in Japan 日本稻田土壤中溶磷细菌的分离与鉴定

IF 2.2 4区环境科学与生态学

Microbes and Environments Pub Date : 2022-05-21 DOI: 10.1264/jsme2.ME21085

Jean Louise Cocson Damo, María Daniela Artigas Ramírez, Shin-ichiro Agake, M. Pedro, Marilyn B. Brown, H. Sekimoto, T. Yokoyama, S. Sugihara, Shin Okazaki, Naoko Ohkama‐Ohtsu

{"title":"Isolation and Characterization of Phosphate Solubilizing Bacteria from Paddy Field Soils in Japan","authors":"Jean Louise Cocson Damo, María Daniela Artigas Ramírez, Shin-ichiro Agake, M. Pedro, Marilyn B. Brown, H. Sekimoto, T. Yokoyama, S. Sugihara, Shin Okazaki, Naoko Ohkama‐Ohtsu","doi":"10.1264/jsme2.ME21085","DOIUrl":"https://doi.org/10.1264/jsme2.ME21085","url":null,"abstract":"Phosphorus (P) is abundant in soil and is essential for plant growth and development; however, it is easily rendered insoluble in complexes of different types of phosphates, which may lead to P deficiency. Therefore, increases in the amount of P released from phosphate minerals using microbial inoculants is an important aspect of agriculture. The present study used inorganic phosphate solubilizing bacteria (iPSB) in paddy field soils to develop microbial inoculants. Soils planted with rice were collected from different regions of Japan. Soil P was sequentially fractionated using the Hedley method. iPSB were isolated using selective media supplemented with tricalcium phosphate (Ca-P), aluminum phosphate (Al-P), or iron phosphate (Fe-P). Representative isolates were selected based on the P solubilization index and soil sampling site. Identification was performed using 16S rRNA and rpoB gene sequencing. Effectiveness was screened based on rice cultivar Koshihikari growth supplemented with Ca-P, Al-P, or Fe-P as the sole P source. Despite the relatively homogenous soil pH of paddy field sources, three sets of iPSB were isolated, suggesting the influence of fertilizer management and soil types. Most isolates were categorized as β-Proteobacteria (43%). To the best of our knowledge, this is the first study to describe the genera Pleomorphomonas, Rhodanobacter, and Trinickia as iPSB. Acidovorax sp. JC5, Pseudomonas sp. JC11, Burkholderia sp. JA6 and JA10, Sphingomonas sp. JA11, Mycolicibacterium sp. JF5, and Variovorax sp. JF6 promoted plant growth in rice supplemented with an insoluble P source. The iPSBs obtained may be developed as microbial inoculants for various soil types with different P fixation capacities.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47554913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Bacterial Community of Water Yam (Dioscorea alata L.) cv. A-19 水山药的细菌群落。19

IF 2.2 4区环境科学与生态学

Microbes and Environments Pub Date : 2022-05-03 DOI: 10.1264/jsme2.ME21062

Shunta Kihara, Kosuke Yamamoto, Atsushi Hisatomi, Yuh Shiwa, Chia-Cheng Chu, Kanako Takada, Michel Ouyabe, Babil Pachakkil, H. Kikuno, N. Tanaka, H. Shiwachi

{"title":"Bacterial Community of Water Yam (Dioscorea alata L.) cv. A-19","authors":"Shunta Kihara, Kosuke Yamamoto, Atsushi Hisatomi, Yuh Shiwa, Chia-Cheng Chu, Kanako Takada, Michel Ouyabe, Babil Pachakkil, H. Kikuno, N. Tanaka, H. Shiwachi","doi":"10.1264/jsme2.ME21062","DOIUrl":"https://doi.org/10.1264/jsme2.ME21062","url":null,"abstract":"The bacterial community of water yam (Dioscorea alata L.) cv. A-19 is vital because it may promote plant growth without the need for fertilization. However, the influence of fertilization practices on the composition and proportion of the bacterial community of water yam cv. A-19 has not yet been extensively examined. Therefore, we herein investigated the diversity and composition of the bacterial community of water yam cv. A-19 cultivated with and without chemical fertilization using amplicon community profiling based on 16S rRNA gene sequences. No significant difference was detected in the growth of plants cultivated with or without chemical fertilization. Alpha diversity indices were significantly dependent on each compartment, and a decrease was observed in indices from the belowground (rhizosphere and root) to aboveground compartments (stem and leaf). The bacterial composition of each compartment was clustered into three groups: bulk soil, rhizosphere and root, and stem and leaf. Chemical fertilization did not significantly influence the diversity or composition of the water yam cv. A-19 bacterial community. It remained robust in plants cultivated with chemical fertilization. The amplicon community profiling of bacterial communities also revealed the dominance of two bacterial clades, the Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium clade and Burkholderia-Caballeronia-Paraburkholderia clade, with and without chemical fertilization. This is the first study to characterize the bacterial community of water yam cv. A-19 cultivated with and without chemical fertilization.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44206780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

NH2OH Disproportionation Mediated by Anaerobic Ammonium-oxidizing (Anammox) Bacteria 厌氧氨氧化菌介导的NH2OH歧化反应

IF 2.2 4区环境科学与生态学

Microbes and Environments Pub Date : 2022-04-12 DOI: 10.1264/jsme2.ME21092

M. Oshiki, Lin Gao, Lei Zhang, S. Okabe

{"title":"NH2OH Disproportionation Mediated by Anaerobic Ammonium-oxidizing (Anammox) Bacteria","authors":"M. Oshiki, Lin Gao, Lei Zhang, S. Okabe","doi":"10.1264/jsme2.ME21092","DOIUrl":"https://doi.org/10.1264/jsme2.ME21092","url":null,"abstract":"Anammox bacteria produce N2 gas by oxidizing NH4+ with NO2–, and hydroxylamine (NH2OH) is a potential intermediate of the anammox process. N2 gas production occurs when anammox bacteria are incubated with NH2OH only, indicating their capacity for NH2OH disproportionation with NH2OH serving as both the electron donor and acceptor. Limited information is currently available on NH2OH disproportionation by anammox bacteria; therefore, the stoichiometry of anammox bacterial NH2OH disproportionation was examined in the present study using 15N-tracing techniques. The anammox bacteria, Brocadia sinica, Jettenia caeni, and Scalindua sp. were incubated with the addition of 15NH2OH, and the production of 15N-labeled nitrogenous compounds was assessed. The anammox bacteria tested performed NH2OH disproportionation and produced 15-15N2 gas and NH4+ as reaction products. The addition of acetylene, an inhibitor of the anammox process, reduced the activity of NH2OH disproportionation, but not completely. The growth of B. sinica by NH2OH disproportionation (–240.3 kJ mol NH2OH–1 under standard conditions) was also tested in 3 up-flow column anammox reactors fed with 1) 0.7 mM NH2OH only, 2) 0.7 mM NH2OH and 0.5 mM NH4+, and 3) 0.7 mM NH2OH and 0.5 mM NO2–. NH2OH consumption activities were markedly reduced after 7 d of operation, indicating that B. sinica was unable to maintain its activity or biomass by NH2OH disproportionation.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41463425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

N2O Reduction by Gemmatimonas aurantiaca and Potential Involvement of Gemmatimonadetes Bacteria in N2O Reduction in Agricultural Soils 金双胞菌对农业土壤N2O的还原作用及双胞菌在N2O还原中的潜在作用

IF 2.2 4区环境科学与生态学

Microbes and Environments Pub Date : 2022-04-12 DOI: 10.1264/jsme2.ME21090

M. Oshiki, Yuka Toyama, T. Suenaga, A. Terada, Y. Kasahara, Takashi Yamaguchi, N. Araki

{"title":"N2O Reduction by Gemmatimonas aurantiaca and Potential Involvement of Gemmatimonadetes Bacteria in N2O Reduction in Agricultural Soils","authors":"M. Oshiki, Yuka Toyama, T. Suenaga, A. Terada, Y. Kasahara, Takashi Yamaguchi, N. Araki","doi":"10.1264/jsme2.ME21090","DOIUrl":"https://doi.org/10.1264/jsme2.ME21090","url":null,"abstract":"Agricultural soil is the primary N2O sink limiting the emission of N2O gas into the atmosphere. Although Gemmatimonadetes bacteria are abundant in agricultural soils, limited information is currently available on N2O reduction by Gemmatimonadetes bacteria. Therefore, the effects of pH and temperature on N2O reduction activities and affinity constants for N2O reduction were examined by performing batch experiments using an isolate of Gemmatimonadetes bacteria, Gemmatimonas aurantiaca (NBRC100505T). G. aurantiaca reduced N2O at pH 5–9 and 4–50°C, with the highest activity being observed at pH 7 and 30°C. The affinity constant of G. aurantiaca cells for N2O was 4.4 μM. The abundance and diversity of the Gemmatimonadetes 16S rRNA gene and nosZ encoding nitrous oxide reductase in agricultural soil samples were also investigated by quantitative PCR (qPCR) and amplicon sequencing analyses. Four N2O-reducing agricultural soil samples were assessed, and the copy numbers of the Gemmatimonadetes 16S rRNA gene (clades G1 and G3), nosZ DNA, and nosZ mRNA were 8.62–9.65×108, 5.35–7.15×108, and 2.23–4.31×109 copies (g dry soil)–1, respectively. The abundance of the nosZ mRNA of Gemmatimonadetes bacteria and OTU91, OUT332, and OTU122 correlated with the N2O reduction rates of the soil samples tested, suggesting N2O reduction by Gemmatimonadetes bacteria. Gemmatimonadetes 16S rRNA gene reads affiliated with OTU4572 and OTU3759 were predominant among the soil samples examined, and these Gemmatimonadetes OTUs have been identified in various types of soil samples.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42373544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5