Materials Express最新文献

{"title":"ATI-2341 TFA promotes repair of damaged endometrium by mediating the differentiation of bone marrow mesenchymal stem cells","authors":"Shiying Guo, Hong Chen, Ying Xu, Doudou Ding, Aihua Chen","doi":"10.1166/mex.2024.2576","DOIUrl":"https://doi.org/10.1166/mex.2024.2576","url":null,"abstract":"Intrauterine adhesion (IUA) is a common complication in endometrial disorder. This study investigated the role of ATI-2341, a functionally selective allosteric regulator of CXCR4 in IUAs, and its interaction with bone marrow-derived mesenchymal stem cells (BMSCs). Following establishment of endometrial injury model, rats BMSCs were treated with ATI-2341 TFA (100 ng/mL). Endometrial tissues of rats with IUA were treated with BMSCs and estrogen, or untreated which was taken as controls. The endometrium thickness was examined and endometrial BMSC proliferation was detected by MTT assay. Levels of MMP-9, TIMP-1, CK, and VIM were determined by Western blot analysis. Treatment with ATI-2341 TFA resulted in significantly decreased level of MMP-9 (0.346±0.036 ng/mL), compared with estrogen treatment (0.467±0.029 ng/mL) and control treatment. Both BMSCs carrying ATI-2341 TFA and estrogen induced increased expression of TIMP-1 (0.734±0.034 ng/mL, 0.618±0.035 ng/mL) and enhanced endometrim growth with thicker endometrium in ATI-2341 TFA group (294.21±59.97 μm). 48 h and 72 h after treatment, ATI-2341 TFA group and estrogen group exhibited increased proliferation rate (P <0.05), and higher rate appeared upon ATI-2341 TFA treatment. Cells in ATI-2341 TFA100 ng/mL and estrogen group were greatly positive for keratin and negative for vimentin. Collectively, ATI-2341 TFA promotes the differentiation of BMSCs into endometrial epithelial cells, enhances repair of damaged endometrium, and alleviate IUA. These findings might underlie a foundation for BMSC-based treatment for endometrial disorder.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"3 4","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139124974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of recombinant human brain natriuretic peptide combined with sacubitril and valsartan sodium tablets on the condition of patients with coronary atherosclerotic heart failure","authors":"Jun Fan, Chunxiao Yan","doi":"10.1166/mex.2024.2589","DOIUrl":"https://doi.org/10.1166/mex.2024.2589","url":null,"abstract":"Coronary atherosclerotic heart failure (CAHF) is a common clinical cardiovascular disease that seriously endangers human health. Combination of recombinant human brain natriuretic peptide and sacubitril-valsartan sodium tablets (rhBNP-SV) may have a positive effect on CAHF patients. However, there have been few research on this. In this study, 120 patients with CAHF were collected, 30 of them were used as control group and 90 patients were randomly divided into sacubitril-valsartan sodium tablet group, rhBNP group and combination group. The cardiac function-related indicators and serum mitochondrial coupling factor 6, ACE and others were examined. The levels of various cardiac function indexes in CAHF patients showed an upward trend and the increase was the most obvious in combination group. Patients in combination group had the longest walking distance within 6 minutes. Levels of the four groups of patients showed a downward trend and the intervention of rhBNP-VS showed the most significant decrease. Proportion of IFN-γ-positive lymphocytes in the total T lymphocytes gradually decreased with different interventions. Under rhBNP-SV intervention, ACE level in patients was significantly inhibited. The treatment of rhBNP-SV improved the exercise capacity of patients, reduced inflammatory response, and regulated ACE expression in patients with CAHF. Furthermore, it reduced the degree of vascular endothelial injury, inhibited the development of the disease, and ultimately enhanced the therapeutic effect to a large extent.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"58 3","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139125192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabonomics of brain tissue in migraine rat model induced by nitroglycerin or cortical spreading depression","authors":"Jiansheng Yang, Di Wu, Liling Zeng, Minzhi Zhong, Tianshan Liang, Honghong Jiang, Qiang Liu, Yanhua Wu, Hui Li","doi":"10.1166/mex.2024.2579","DOIUrl":"https://doi.org/10.1166/mex.2024.2579","url":null,"abstract":"This study analyzes the differences in potential biomarkers of migraine models constructed by two commonly used modeling methods through metabolomics research. Migraine models were constructed by nitroglycerin (NTG) induction or mechanical stimulus-induced cortical spreading depression (CSD) in rats. Multivariate analysis was used for metabolomic analysis. Compared with control group, NTG migraine group had 1 different metabolite up-regulated and 14 down-regulated. Compared with NTG migraine group, 13 differential metabolites in the CSD migraine model group were up-regulated and 12 were down-regulated. Both nitroglycerin-induced and mechanical stimulation-induced CSD can construct migraine rat models, and the biomarkers of the two models are different.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"112 7","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139126074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simvastatin mitigates vascular cognitive impairment in rat’s hippocampus in lacunar cerebral infarction","authors":"Sihan Chen, Yening Zhu, Zhuqin Li, Donger Chen","doi":"10.1166/mex.2024.2612","DOIUrl":"https://doi.org/10.1166/mex.2024.2612","url":null,"abstract":"Simvastatin is used to treat Alzheimer’s disease with impaired cognitive function through blocking of PI3K/Akt signaling to inhibit survival of new neurons and growth of neurons. This study investigated the potential of simvastatin on vascular cognitive impairment (VCI) caused by lacunar cerebral infarction (LCI) in the hippocampus of rats. After establishment of LCI models, rats were administered with NaOH solution (model group), simvastatin (simvastatin group), and PI3K/Akt pathway inhibitor (inhibitor group), respectively, and 10 healthy rats served as control group. Upon treatment, Y-maze and Morris water maze detected spatial cognition ability of rats; while cleaved caspase-3 staining was used to assess apoptosis in the hippocampus. Western blot analysis detected Akt and ERK1/2 phosphorylation levels and enzyme-linked immuno sorbent assay (ELISA) determined the content of brain-derived neurotrophic factor (BDNF). The Y maze Morris water maze confirmed impairment of long-term memory abilities in the LCI rats from the model group. Treatment with simvastatin significantly improved correct rate of arm alternates in rats and shorted the stage latency in the hidden platform experiment. The number of apoptotic cells increased in the hippocampal DG sub-granular area of rats with LCI, and BDNF level was significantly reduced. Administration of simvastatin suppressed cell apoptosis and increased BDNF level when improving phosphorylation level of Akt and ERK1/2 as well. Cerebral ischemia following LCI affects short and long-term memory, which leads to VCI. Simvastatin increases Akt and ERK1 in PI3K/Akt pathway, and hence up-regulates hippocampal BDNF levels, which promotes the regeneration of rat hippocampal nerve cells to relieve VCI.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"8 15","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139125013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of initial twins on mechanical properties of additive manufacturing CoCrNi alloy and molecular dynamic simulation","authors":"Xu Kong, Haiyi Cheng, Yuying Jiang, Di Hu","doi":"10.1166/mex.2024.2593","DOIUrl":"https://doi.org/10.1166/mex.2024.2593","url":null,"abstract":"The influence of twins on laser additive CoCrNi MEA was analyzed by experiments and molecular dynamic simulation. Results showed that the traditional laser additive CoCrNi MEA was prone to slip in the (111) direction. EBSD analysis showed an obvious 60°(111} axial angle pair relationship, which is related to the slower cooling rate. The results of molecular dynamic simulation showed that the yield strength of CoCrNi MEA increases first and then decreases, and the material has a critical twin density. When the twin density is less than the critical twin density, the strength of the material increases due to the thinning effect of the twin boundary on the grain. When the twin density is greater than the boundary twin density, the nucleation and proliferation of the twin boundary and the junction of the two become the dominant factors of the material deformation. When the twin density is far from the critical value, the twin spacing becomes smaller, the dislocation sources increase, the dislocation nucleation and proliferation increase, and the strength of the material decreases.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"14 11","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139126380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumin targets miR-1297 to promote mitochondrial apoptosis and cisplatin sensitization of lung cancer stem cells","authors":"Jiayang Sun, Luzhi Gang, Chunlin Li","doi":"10.1166/mex.2024.2597","DOIUrl":"https://doi.org/10.1166/mex.2024.2597","url":null,"abstract":"The medicinal value of curcumin has been confirmed in many diseases. This study explores miR-1297’s role in lung cancer cells. The targeting regulation between miR-1297 and BH3-only was analyzed by luciferase activity. Cisplatin (DDP)-resistant cell lines A549/DDP and NCI-H460/DDP were established followed by analysis of cell proliferation activity, BH3-only expression, and cell proliferation by EdU staining. miR-1297 targets BH3-only. miR-1297 expression in A549/DDP and NCI-H460/DDP cells was significantly increased while BH3-only was reduced. Same dose of DDP inhibited the proliferation of A549/DDP and NCI-H460/DDP cells. BH3-only in A549/DDP and NCI-H460/DDP cells in miR-1297 inhibitor transfection group was significantly increased, apoptosis was increased, proliferation was weakened, and drug resistance was reduced. Lung cancer DDP resistance is related to the expression of miR-1297. Down-regulation of miR-1297 can reduce the DDP drug resistance by increasing the expression of BH3-only.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"14 24","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139129650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression analysis of insulin-like growth factor 1, neutrophil/lymphocyte ratio, and inflammatory factors in children with Mycoplasmal pneumonia","authors":"Zhaoyan Wang, Xian Wang, Wanglan Zhao, Jun Luo","doi":"10.1166/mex.2023.2572","DOIUrl":"https://doi.org/10.1166/mex.2023.2572","url":null,"abstract":"Mycoplasma pneumonia (MPP) belongs to atypical pneumonia, which is caused by Mycoplasma pneumonia infection, accounting for 10–40% of children’s community pneumonia. It is more common in children at preschool age. The clinical symptoms of MPP are varied and\u0000 difficult to cure. The research analyze the expression of inflammatory factors in MPP, neutrophil/lymphocyte ratio (NLR), insulin-like growth factor 1 (IGF1). The aim is to provide relevant basis for treatment and diagnosis of the disease. 40 normal children were selected as the control group.\u0000 78 children with MPP were selected as the observation group. The levels of interleukin-6 (IL-6) and interleukin-2 (IL-2) in peripheral blood were detected. Platelet/lymphocyte ratio (PLR) and NLR were tested. IGF1 expression was determined by ELISA. The correlation between IGF1, IL-6, IL-2NLR,\u0000 PLR and the severity of MPP was analyzed. IL-2, NLR, and PLR significantly increased. The IGF1 in MPP group was lower than control group (P <0.05). IL-6 level in two group was no significant change. NLR, PLR and IL-2 were negatively correlated with IGF1. NLR, IL-2, PLR were positively\u0000 correlated with MPP severity (P <0.05). NLR, PLR, and IL-2 were upregulated, whereas IGF1 was declined in MPP, which can be used to evaluate the progression of MPP.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":" 52","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138612269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel nano-SiRNA delivery system loaded with anti-ESR1 siRNA and its application as a cyclosporine drug carrier in dry eye caused by diabetes","authors":"Jing He, Yunzhi Lin, Fen Ye, Ying Gao, Ting Yu, Bilian Ke, Weihong Zhou","doi":"10.1166/mex.2023.2561","DOIUrl":"https://doi.org/10.1166/mex.2023.2561","url":null,"abstract":"Dry eye is a relatively common clinical condition caused by abnormal fluid dynamics and tear quality, for which effective pharmacological treatment is lacking. In this study, a new nano-siRNA delivery system loaded with anti-ESR1 siRNA was designed and synthesized, and its effect in\u0000 the treatment of dry eye was evaluated. The system significantly inhibited the NaCl-induced increase in inflammatory factors, thereby suppressing the inflammatory response. In addition, the system downregulated the expression level of MMP-9 and STRA6. This system could be developed as a drug\u0000 for the treatment of diabetes-induced dry eye.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":" 31","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138614287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of metformin nanoemulsion through AMP-activated protein kinase (AMPK)-mediated autophagy on diabetic neuropathy","authors":"Fengmin Liu, Siyun Wang, Xiaojuan Du, Diya Xie","doi":"10.1166/mex.2023.2560","DOIUrl":"https://doi.org/10.1166/mex.2023.2560","url":null,"abstract":"Metformin (MET) is the preferred first-line treatment for patients with type 2 diabetes. However, the mechanism of diabetic peripheral neuropathy (DPN) is still unclear. To improve the oral utilization of metformin, a metformin nanoemulsion (MET-NE) was prepared to investigate its effects\u0000 on DPN and its underlying mechanism. In this study, a DPN model was established in Wistar rats induced by streptozotocin (STZ). The diabetic rats were randomly divided into four groups: the diabetic model group (DM group), the metformin tablet-treated group (MET group), the metformin nanoemulsion-treated\u0000 group (MET-NE group), and a normal control group consisting of five normal Wistar rats. All groups were administered the treatment orally for a period of 10 weeks. The findings of the study demonstrated that both MET and MET-NE significantly reduced blood glucose levels, glycated serum protein\u0000 levels, food intake, and water intake in DM rats. It was also observed that MET-NE was more effective than MET in reducing blood glucose levels. Additionally, both MET and MET-NE treatments significantly increased the motor nerve conduction velocity (MNCV), sensory nerve conduction velocity\u0000 (SNCV), and sensory nerve action potential (SNAP) amplitude in DM rats. Furthermore, these treatments improved mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL), resulting in a reduced sensitivity to pain stimuli. Moreover, both MET and MET-NE treatments promoted the\u0000 phosphorylation of AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC) proteins, as well as increased the activity of autophagic proteins in the sciatic nerve. However, no significant differences were observed between MET and MET-NE treatments in terms of these effects. In\u0000 conclusion, the MET-NE demonstrated a rapid decrease in blood glucose levels and improved glucose tolerance and metabolism, which was found to be superior to MET. Furthermore, MET-NE significantly improved the neurophysiological function and sciatic nerve pain threshold in DPN rats. These\u0000 beneficial effects may be attributed to the regulation of AMPK-mediated autophagy by MET-NE.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":" 2","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138615692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of a bearing capacity testing model for reinforced bridges based on material performance deterioration","authors":"Xia Zhang, Lei Zhang, Rongbin Huang, Xianghui Zhang","doi":"10.1166/mex.2023.2556","DOIUrl":"https://doi.org/10.1166/mex.2023.2556","url":null,"abstract":"Reinforced concrete beam bridges have been extensively utilized in transportation engineering due to their advantages such as simplified material selection and low cost. However, the mechanical performance of steel bars and concrete deteriorates over time due to factors such as prolonged\u0000 bridge service life, fluctuations in environmental temperature and humidity, and other influences. To achieve a precise and effective prediction of the bearing capacity of reinforced concrete structures, this study proposes a method for assessing bearing capacity in the context of material\u0000 performance degradation. The study commenced by examining the factors that impact the degradation of bridge performance and subsequently utilized material performance test values along with predicted and corrected values to estimate the reduced bearing capacity of the bridge. Additionally,\u0000 the mechanical and bonding properties of modified reinforced concrete materials were calculated. The experiment results demonstrate that the calculated values from the modified model closely align with the measured values with a differential range of 0.06 to 0.38. At a micro level, nano-SiO2\u0000 modification enhances rubber concrete by improving the compactness of the matrix and enhancing bond strength between steel fiber-and-nanosilica-reinforced crumb rubber concrete and deformed steel bars. The revised model in this study exhibits excellent predictive capability for concrete strength,\u0000 thereby enhancing the accuracy of outcomes in evaluating bridge technical conditions. This method holds potential for practical engineering applications and scientific research on bridge evaluation.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":" 34","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138616387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}