Materials Express最新文献

{"title":"The mechanism of metallothionein MT1M-mediated PI3K/AKT signaling pathway in the regulation of diabetic retinopathy","authors":"Lu Gao, Fangling Song, Ting Liu","doi":"10.1166/mex.2024.2594","DOIUrl":"https://doi.org/10.1166/mex.2024.2594","url":null,"abstract":"Metallothionein (MT1M) is associated with tumors and autoimmune diseases. However, its role in DR has not yet been elucidated. DR and normal rat retinal endothelial cells (RECs) were isolated and cultured. DR Rat RECs achieved gene regulation by transfecting MT1M plasmid. PCR and MTT were used to detect MT1M expression, cell proliferation. Flow cytometry was used to detect cell apoptosis. Lactate dehydrogenase (LDH), superoxide dismutase (SOD) and reactive oxygen species (ROS) were detected by MTT. The expression of VEGF and PI3K/AKT signaling pathway was detected by Western blot. The levels of inflammatory factors TNF-α and IL-1β were detected by ELISA. The results showed that the expression of MT1M was reduced in the RECs of DRC rats compared to the normal control group, cell proliferation was enhanced, SOD activity was reduced, LDH and ROS levels were increased, TNF-α and IL-1β secretion increased, and vascular endothelial growth factor (VEGF), PI3K/AKT expression increased (P < 0.05). However, transfection with MT1M plasmid could significantly inhibit cell proliferation, increase SOD activity, reduce LDH and ROS levels, reduce TNF-α and IL-1β secretion, and reduce VEGF and PI3K/AKT expression (P <0.05). The expression of MT1M is reduced in RECs of DR rats. Up-regulation of MT1M can regulate DR3K/AKT signaling pathway and oxidative/antioxidant balance, alter VEGF expression, inhibit inflammation, regulate the growth and proliferation of RECs, and delay DR lesions.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"58 5","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139127683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Positive effects of dietary honey and aflatoxin B1 on serum enzymes, superoxide dismutase activity, β-glucuronidase enzyme activity, and colonic probiotic bacteria on rats","authors":"Alaa Baazeem, M. Helal, R. Sami, G. Alshehry, Eman Algarni, Uguru Hilary, Fadi Baakdah, S. Alharthy, Doaa Mahmoud Johari","doi":"10.1166/mex.2024.2584","DOIUrl":"https://doi.org/10.1166/mex.2024.2584","url":null,"abstract":"Dietary honey as a natural product can attenuate the inflammatory process and prevent several diseases. The current research aimed to assess some positive effects of dietary honey and aflatoxin B1 on serum enzymes, superoxide dismutase activity, β-glucuronidase enzyme activity, and colonic probiotic bacteria in rats. Four kinds of honey were coded as NSH, MOH, SIH, and PUH for Nigella sativa, moringa, pumpkin, and Sidr honey, respectively. Aflatoxin B1 (AFB1) was daily administered orally for rats with a dose of 200 μg/kg until 90 days. The rat groups’ body weights at the beginning and end of the experiment ranged from 311.29 g to 327.55 g, and 302.97 g to 342.77 g respectively. Dietary honey decreased the values of some serum enzymes and protected the animals from AFB1 hepatotoxicity compared with the positive group (+). The concentrations of liver superoxide dismutase activity (SOD) among rat groups ranged from 19.55 u ·g in MOH+AFB1 rat group to 27.21 u·g in SIH+AFB1 rat group. Dietary honey was reported to defend the liver against aflatoxins and enhance the gut microflora as β-glucuronidase activity and colonic probiotic bacteria in rats.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"27 10","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139125795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of Kudzu vine oral formulation and its application against doxorubicin-induced cardiotoxicity via alteration of gut microbiota","authors":"Qingmei Wang, Xuanguo Zhang","doi":"10.1166/mex.2024.2533","DOIUrl":"https://doi.org/10.1166/mex.2024.2533","url":null,"abstract":"In this experimental protocol, we scrutinized the preventive effect of Kudzu vine extract on DOX-induced cardiac toxicity via alteration of gut microbiota. Sprague-Dawley (SD) was used in this protocol and the rats were divided into different groups and received the DOX (3 mg/kg) for induction the cardiac remodeling and Kudzu vine extract (50, 100 and 200 mg/kg) administration was used for the estimation of cardioprotective potential for 5 weeks. Afterward, biochemical parameter, electrolyte, antioxidant, cytokines and inflammatory parameters were estimated. Kudzu vine remarkably improved the body weight and declined the heart weight. Kudzu vine extract significantly (P <0.001) suppressed the level of CK-MB, LDH, CK, cTnT and BNP. Kudzu vine significantly (P <0.001) improved the level of Na+, Ca2+, K+ and Cl− and decreased the level of urea, magnesium, total protein, albumin and globulin. Kudzu vine remarkably altered the lipid and antioxidant parameters. Additionally, Kudzu vine extract suppressed the level of TNF-α, IL-1β, IL-6 and improved the level of TGF-β, IL-10. DOX-induced cardiac dysfunction exhibited the altered relative abundance of different bacteria and Kudzu vine remarkably restore the relative abundance of different bacteria. Therefore, Kudzu vine extract has beneficial effects against DOX-induced cardiac toxicity via alteration of gut microbiota.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"27 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139126221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism of montelukast on autophagy and apoptosis of airway epithelial cells through STAT3-RORγt-IL-17/IL-23 signaling pathway","authors":"Hui Xu, Xiurong Wang, Bin Du","doi":"10.1166/mex.2024.2596","DOIUrl":"https://doi.org/10.1166/mex.2024.2596","url":null,"abstract":"This study investigates the mechanism of montelukast intervention on autophagy and apoptosis of airway epithelial cells. Construction of HBE cell building model, which were intervened by montelukast. The proliferation of human 16HBE cells was detected using MTT method and β-catenin level was detected. The cellular cycle distribution, autophagy and apoptosis were detected using flow cytometry. And expressions of Transcriptional activator 3 (STAT3)-Retinoic acid-associated nuclear orphan receptor (RORγt)-interleukin 17 (IL-17)/interleukin 23 (IL-23) signaling related proteins were measured using Western blot. Montelukast inhibited the proliferation of human 16HBE cells and its inhibition rate and action concentration showed time and dose dependence. The half maximal inhibitory concentrations (IC50) were (12.8±0.67) μmol/L at 24 h, (8.8±0.43) μmol/L at 48 h and (6.6±0.42) μmol/L at 72 h, respectively. Montelukast induced 16HBE cellular cycle to arrest in G2/M phase dose-dependently (5, 10 and 20 μmol/L) (P <0.05) and simultaneously increased apoptosis rate (P < 0.05). 40 μL montelukast had a protective effect on 16HBE cells. In addition, montelukast reduced β-catenin level, which suggested that STAT3-RORγt-IL-17/IL-23 signaling pathway might be inhibited. Meanwhile, montelukast reduced the expressions of STAT3, P-STAT3, RORγt (RORβ), c-myc and survivin and increased protein expressions of GSK-3 (RORα) and Th17, but had no effect on the total RORγt level. Montelukast may effectively promote the apoptosis of 16HBE airway epithelial cells via inhibition of STAT3-RORγt-IL-17/IL-23 signaling.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"47 16","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139126611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term monitoring of SF6 decomposition gases and water molecules using TiO2-doped SnSe monolayer for generator circuit breakers","authors":"Rufei He, Li Cheng, Xiaofeng Huang, Hao Xu, Xiaojing Zhang, Xiaobo Zhang","doi":"10.1166/mex.2024.2590","DOIUrl":"https://doi.org/10.1166/mex.2024.2590","url":null,"abstract":"In generator circuit breakers, monitoring the decomposition of Sulfur Hexafluoride (SF6) gas is a primary method to determine insulation and fault conditions. The presence of SF6 combined with H2O impurities also significantly impacts the degradation of equipment insulation. However, there is a lack of research on the simultaneous monitoring of both SF6 decomposition and H2O. To improve the system’s rapid recoverability, recyclability, and long-term usability, a SnSe monolayer doped with TiO2 nanoparticles (SnSe–TiO2) has been proposed as an SF6 gas decomposer detection sensor with humidity detection capabilities. The SnSe–TiO2 monolayer significantly enhances the conductivity and increases its adsorption energy for H2O (73.2%), H2S (13.54%), HF (59.70%), SO2 (96.33%), and SOF2 (52.04%). Furthermore, this material can be utilized for long-term cyclic gas monitoring as the SnSe–TiO2 monolayer is physically adsorbed to these gases and can be rapidly desorbed. This study establishes a theoretical foundation for the future advancement of gas detection sensors for insulating gases in circuit breakers.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"59 49","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139126673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation and continuous improvement of magnetic components material design skills in interdisciplinary switched-mode power supply","authors":"Yi Kuang, Zhiyong Zhang, Bin Duan","doi":"10.1166/mex.2024.2581","DOIUrl":"https://doi.org/10.1166/mex.2024.2581","url":null,"abstract":"The design of switched-mode power supplies (SMPSs) is a material-electrical engineering interdisciplinary problem. The design of magnetic component materials can significantly affect SMPSs’ performances. In engineering education, the design and development of solutions is an important skill for engineering students. However, the traditional engineering experiments curriculum is shown as validation and single-disciplinary experiments as well as one-sided assessment results. To foster open-ended exploration, enhance students’ material design skills, and improve skills adaptation, students must tackle real engineering problems and develop practical design solutions. This paper proposes an exploratory factor analysis and knowledge graph-based method for evaluating and continuously improving magnetic components material design skills in the context of SMPS design tasks. First, we used the multiple imputation method to address the missing data. Each imputed data was analyzed to extract factors through parallel analysis, ordinary least squares estimation, and target rotation. Then, we identified four sub-skills: efficiency design skill, passive device design skill, power magnetic reduction design skill, and power economy design skill. The RMSEA for the four-factor model is 0.042, suggesting a good fit to the data. We established the relationships between these sub-skills and SMPS performance metrics. Furthermore, the average of the factor scores from each of the imputed datasets and the SMPS design constraints were combined to obtain the cut-off scores to evaluate engineering students’ achievements in these sub-skills. Finally, we constructed an SMPS magnetic components material knowledge graph, which could recommend specific experimental tasks, relevant knowledge areas, and SMPS performance metrics, providing personalized guidance to designers.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"21 13","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139126342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect and mechanism of a subanesthetic dose of esketamine on postoperative neurocognitive disorders through the regulating of the TLR4/NF-κB pathway in microglia","authors":"Yang Zhang, Mengying Xiong, Shibiao Chen, Ningyan Wang, Xiuhong Wang","doi":"10.1166/mex.2024.2583","DOIUrl":"https://doi.org/10.1166/mex.2024.2583","url":null,"abstract":"Perioperative neurocognitive disorders (PND) are postoperative or anesthesia-related complications of the central nervous system, characterized by intelligence decline, memory loss, attention deficit, impaired decision-making skills, reduced language comprehension performance, and psychological disorders. This study aims to investigate the impact of a subanesthetic dose of esketamine on PND in aged mice by regulating the microglia toll-like receptor 4 (TLR4)/nuclear factor κB (NF-κB) pathway. An exploratory laparotomy model was established in 18-month-old C57BL/6 mice post-anesthesia. Behavioral evaluations such as open field tests and Morris water maze tests for autonomous activity and spatial learning and memory ability. Different esketamine doses were administered to aged mice, followed by behavioral assessment for autonomous activity and learning/memory abilities. Nucleic acid extraction employed magnetic nanoparticles, while qRT-PCR measured mRNA levels of tumor necrosis factor-α, interleukin-6, and interleukin-1β. Western Blot detected the Iba-1 and TLR4 protein expression and phosphorylation status of the P65 protein. Immunofluorescence determined the fluorescence intensity of Iba-1 and P-NF-κBp65. MTT assay evaluated cell viability. As a result, we demonstrated that a subanesthetic dose of esketamine effectively inhibited microglia activation and TLR4/NF-κB signaling pathway both in vivo and in vitro, resulting in decreased levels of inflammatory factors and improved neuroinflammatory responses, ultimately alleviating postoperative neurocognitive dysfunction. Notably, our findings revealed that laparotomy exploratory surgery-induced cognitive dysfunction, which can be improved by esketamine’s neuroprotective effects. Furthermore, our study also highlighted the protective effect of esketamine against lipopolysaccharide-induced inflammatory response in BV2 cells by inhibiting the TLR4/NF-κB signaling pathway.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"81 6","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139127601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of electrospun pluronic F68/polyvinyl alcohol nanofibers composites containing titanium dioxide, silver, and cobalt nanoparticles for biomedical applications","authors":"Mahir Es-Saheb, Yasser Fouad, Khalid A. Ibrahim","doi":"10.1166/mex.2024.2609","DOIUrl":"https://doi.org/10.1166/mex.2024.2609","url":null,"abstract":"Nanoscale drug delivery systems and biomedical sensors are witnessing huge research interests. Polymeric nanoparticles, nanofibers, liposomes, and nano-emulsion are regarded as novel sensors and drug delivery systems including the various types of wound dressings formulated by micro/nanofibers, hydrogels, hydrocolloids, films, foams, and sponges. Consequently, the electro-spun nanofibrous mats received the most attention since the drugs and nanoparticles embedded nanofibers have been recognized as the potential candidates for sensors and wound dressing applications. These composite materials have superior surface area-to-volume ratio, high nano-porosity, distinct skin extracellular matrix structure, and ease of electro-spinning, which also supports a prolonged drug release. In this study the main advantages of the nano-fibrillary network and the active substances that can be incorporated in fabricating the composite nanofibers as sensors and wound dressing materials are discussed. The promising “Pluronic F68” and polyvinyl alcohol (PVA), materials are used including nanoparticles (NPs) of titanium dioxide (TiO2), silver, and cobalt. The difficulties that arise by converting the Pluronic into nanofibers via electrospinning due to its low melting point and beads formation and the blending of this material with polyvinyl alcohol (PVA) are discussed. The electro-spinability of the pure Pluronic, PVA and their blends are evaluated. The microstructures and morphologies of the fabricated composite nanofibers and structures are characterized using scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential thermal analysis (DSC), Fourier transform infrared (FTIR) spectroscopy, and transmission electron microscopy (TEM). The fabricated blended nanofibers were found to be compact and entangled having a diameter from 50 nm to 80 nm and length ranging from 1 to 3 μm. Meanwhile, the pure Pluronic produced nano-beads with an average size of 40 nm. These nanocomposites can be used in biomedical applications such as biosensors by embedding nanoparticles of TiO2, silver, and cobalt and for antibacterial treatment in woundcare dressings.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"65 8","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139128828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduction of inflammatory response after ischemia-reperfusion injury in rats with panax notoginseng saponins by regulating nucleotide-bound oligomerized domain-like receptor protein 1(NLRP1)/Caspase-1 signaling via Cezanne","authors":"Shoujian Zong, Xiaojie Hu, Shouwei Zong, Guizhi Sun","doi":"10.1166/mex.2024.2595","DOIUrl":"https://doi.org/10.1166/mex.2024.2595","url":null,"abstract":"Acute ischemic stroke (AIS) is featured as damage of blood-brain barrier in severe cases, which leads to brain tissue damage. Panax notoginseng saponins (PNS), because of its anti-inflammatory effect, can reduce the inflammatory response caused by ischemia-reperfusion, thereby reducing the degree of damage to the blood-brain barrier. Studies on AIS have confirmed that, Cazenne has an anti-inflammatory effect, but specific mechanism by which PNS regulates Cazenne to reduce the inflammatory response after cerebral ischemia-reperfusion injury (IRI) is still unclear. Therefore, this study explored PNS’ role in inhibiting the inflammatory response after cerebral IRI through Cezanne. Sprague-Dawley (SD) rats were randomly grouped and then assigned into sham operation group (sham), model group (MCAO), model+PNS (MCAO+PNS), model+PNS+Cezanne silencing lentivirus (MCAO+PNS+shCezanne), and model+PNS+empty carrier (MCAO+PNS+shNC). Neurological function was scored by mNSS while cerebral infarction volume was tested by TCC staining. The brain was tested by dry and wet method, while levels of inflammatory factors and NLRP1 and Caspase-1 were detected by ELISA, Western blot, and Cazenne expression was detected by immunofluorescence. PNS reduced the expression of pro-inflammatory factors in MCAO rats, by mainly downregulating NLRP-1 and Caspase-1. By upregulating Cezanne and inflammatory factors downstream NLRP1/Caspase-1 pathway was inhibited, thereby improving the inflammation in the rats after IRI. PNS inhibited the activity of NLRP1/Caspase-1 signaling pathway through Cezanne, thereby reducing inflammatory factors in the brain after cerebral IRI, and reducing inflammatory response.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"16 12","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139127889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yb@TiO2 nanoparticles as a SELDI-TOF MS matrix for the simultaneous measurement of Tetrabromobisphenol A and Perfluorooctane sulfonate","authors":"Jing Yang, Chunhua Luo, Lihao Xiong, Lichun Fu, Xiaohu Luo, Linxiao Gao, Huang Zhou, Lixia Yang, Fei Yang, Deshuai Zhen","doi":"10.1166/mex.2024.2591","DOIUrl":"https://doi.org/10.1166/mex.2024.2591","url":null,"abstract":"Ytterbium modified TiO2 nanoparticles (Yb@TiO2), synthesized by a sol–gel process, were employed as an efficient adsorbent and matrix for the analysis of Perfluorooctane sulfonate (PFOS) and Tetrabromobisphenol A (TBBPA) via surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS). The detection limits for PFOS and TBBPA are achievement with 0.01 pg ·mL−1. The results of the analysis of camellia oil samples show good recovery (80.3%–86.8%) with a low detection limit for TBBPA.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"26 12","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139129054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}