Preparation of Kudzu vine oral formulation and its application against doxorubicin-induced cardiotoxicity via alteration of gut microbiota

In this experimental protocol, we scrutinized the preventive effect of Kudzu vine extract on DOX-induced cardiac toxicity via alteration of gut microbiota. Sprague-Dawley (SD) was used in this protocol and the rats were divided into different groups and received the DOX (3 mg/kg) for induction the cardiac remodeling and Kudzu vine extract (50, 100 and 200 mg/kg) administration was used for the estimation of cardioprotective potential for 5 weeks. Afterward, biochemical parameter, electrolyte, antioxidant, cytokines and inflammatory parameters were estimated. Kudzu vine remarkably improved the body weight and declined the heart weight. Kudzu vine extract significantly (P <0.001) suppressed the level of CK-MB, LDH, CK, cTnT and BNP. Kudzu vine significantly (P <0.001) improved the level of Na+, Ca2+, K+ and Cl− and decreased the level of urea, magnesium, total protein, albumin and globulin. Kudzu vine remarkably altered the lipid and antioxidant parameters. Additionally, Kudzu vine extract suppressed the level of TNF-α, IL-1β, IL-6 and improved the level of TGF-β, IL-10. DOX-induced cardiac dysfunction exhibited the altered relative abundance of different bacteria and Kudzu vine remarkably restore the relative abundance of different bacteria. Therefore, Kudzu vine extract has beneficial effects against DOX-induced cardiac toxicity via alteration of gut microbiota.