Materials science & engineering. C, Materials for biological applications最新文献

{"title":"Mg-Fe layered double hydroxides modified titanium enhanced the adhesion of human gingival fibroblasts through regulation of local pH level","authors":"Yijia Yin ,&nbsp;Linjia Jian ,&nbsp;Baoe Li ,&nbsp;Chunyong Liang ,&nbsp;Xianglong Han ,&nbsp;Xuefeng Zhao ,&nbsp;Donghui Wang","doi":"10.1016/j.msec.2021.112485","DOIUrl":"10.1016/j.msec.2021.112485","url":null,"abstract":"<div><p>The durability of dental implants is closely related to osseointegration and surrounding soft tissue sealing. Appropriate local pH favors fibroblasts adhesion and contributes to soft tissue sealing. Layered double hydroxides (LDHs) are characterized by adjustable alkalinity, offering a possibility to investigate the influence of pH on cellular behaviors. Herein, we fabricated Mg<img>Fe LDHs modified titanium. During calcination, the local pH value of LDHs increase, without altering other physics and chemical properties via OH⁻ exchange mechanism. In vitro studies showed that LDHs films calcined at 250 °C for 2 h provide a local pH of 10.17, which promote early adhesion, proliferation, and type I collagen expression of human gingival fibroblasts (hGFs) through the formation of focal adhesion complex and activation of focal adhesion kinase related signaling pathways. In conclusion, endowing the titanium surface with appropriate alkalinity by Mg<img>Fe LDHs films enhances the adhesion of hGFs, providing a new strategy of designing multifunctional biomaterials for soft tissue sealing around dental implants.</p></div>","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":"131 ","pages":"Article 112485"},"PeriodicalIF":7.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0928493121006251/pdfft?md5=86d4d7d83df68f4a665221d5c5c607cc&pid=1-s2.0-S0928493121006251-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39686032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular matrix-inspired surface coatings functionalized with dexamethasone-loaded liposomes to induce osteo- and chondrogenic differentiation of multipotent stem cells","authors":"Yazmin A. Brito Barrera ,&nbsp;Catharina Husteden ,&nbsp;Jumanah Alherz ,&nbsp;Bodo Fuhrmann ,&nbsp;Christian Wölk ,&nbsp;Thomas Groth","doi":"10.1016/j.msec.2021.112516","DOIUrl":"10.1016/j.msec.2021.112516","url":null,"abstract":"<div><p>Biomimetic surface coatings can be combined with conventional implants to mimic the extracellular matrix (ECM) of the surrounding tissue to make them more biocompatible. Layer-by-layer technique (LbL) can be used for making surface coatings by alternating adsorption of polyanions and polycations from aqueous solutions without need of chemical reactions. Here, polyelectrolyte multilayer (PEM) systems is made of hyaluronic acid (HA) as polyanion and Collagen I (Col) as polycation to mimic the ECM of connective tissue. The PEM are combined with dexamethasone (Dex)-loaded liposomes to achieve a local delivery and protection of this drug for stimulation of osteo- and chondrogenic differentiation of multipotent stem cells. The liposomes possess a positive surface charge that is required for immobilization on the PEM. The surface properties of PEM system show a positive zeta potential after liposome adsorption and a decrease in wettability, both promoting cell adhesion and spreading of C3H10T1/2 multipotent embryonic mouse fibroblasts. Differentiation of C3H10T1/2 was more prominent on the PEM system with embedded Dex-loaded liposomes compared to the basal PEM system and the use of free Dex-loaded liposomes in the supernatant. This was evident by immunohistochemical staining and an upregulation of the expression of genes, which play a key role in osteogenesis (RunX2, ALP, Osteocalcin (OCN)) and chondrogenesis (Sox9, aggrecan (ACAN), collagen type II), determined by quantitative Real-time polymerase chain reaction (qRT-PCR) after 21 days. These findings indicate that the designed liposome-loaded PEM system have high potential for use as drug delivery systems for implant coatings that can induce bone and cartilage differentiation needed for example in osteochondral implants.</p></div>","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":"131 ","pages":"Article 112516"},"PeriodicalIF":7.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0928493121006561/pdfft?md5=07ee6db2ba7fc072e78f206020231ada&pid=1-s2.0-S0928493121006561-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39686245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced osteogenesis and angiogenesis of calcium phosphate cement incorporated with zinc silicate by synergy effect of zinc and silicon ions","authors":"Teliang Lu ,&nbsp;Jing Zhang ,&nbsp;Xinyuan Yuan ,&nbsp;Chenyu Tang ,&nbsp;Xiaolan Wang ,&nbsp;Yu Zhang ,&nbsp;Kun Xiong ,&nbsp;Jiandong Ye","doi":"10.1016/j.msec.2021.112490","DOIUrl":"10.1016/j.msec.2021.112490","url":null,"abstract":"<div><p>Calcium phosphate cement (CPC) with good injectability and osteoconductivity plays important roles in bone grafting application. Much attention has been paid to achieve multifunctionality through incorporating trace elements into CPC. Silicon and zinc can be used as additives to endow CPC with biological functions of osteogenesis, angiogenesis and anti-osteoclastogenesis. In this study, zinc and silicate ions were co-incorporated into CPC through mixing with submicron zinc silicate (Zn₂SiO₄, ZS) to obtain zinc silicate-modified CPCs (ZS/CPCs) with different contents. The results revealed that the addition of ZS increased the compressive strength, prolonged the setting time, and densified the structure of CPC. Low addition content of ZS facilitated the formation of surface apatite layer in the early mineralization stage. Incorporating ZS significantly induced osteogenesis of mouse bone marrow stromal cells (mBMSCs) and angiogenesis of human umbilical vein endothelial cells (HUVECs), and moreover, restricted osteoclastogenesis of Raw 264.7 <em>in vitro</em>. Silicate and zinc ions could be steadily released from ZS/CPCs into the culture medium. With the synergistic effect of silicate and zinc ions, ZS/CPCs provided an appropriate microenvironment for the immune cells to facilitate the osteogenesis of mBMSCs and angiogenesis of HUVECs further. Taken together, it can be concluded that incorporating ZS is an effective way to endow CPC with multifunctionality and better bone regeneration for bone defect repair.</p></div>","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":"131 ","pages":"Article 112490"},"PeriodicalIF":7.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0928493121006305/pdfft?md5=5d3f0e7f2c4551b9ba44768a40c3c92f&pid=1-s2.0-S0928493121006305-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39686417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sonochemically engineered nano-enabled zinc oxide/amylase coatings prevent the occurrence of catheter-associated urinary tract infections","authors":"Aleksandra Ivanova ,&nbsp;Kristina Ivanova ,&nbsp;Ilana Perelshtein ,&nbsp;Aharon Gedanken ,&nbsp;Katerina Todorova ,&nbsp;Rositsa Milcheva ,&nbsp;Petar Dimitrov ,&nbsp;Teodora Popova ,&nbsp;Tzanko Tzanov","doi":"10.1016/j.msec.2021.112518","DOIUrl":"10.1016/j.msec.2021.112518","url":null,"abstract":"<div><p>Catheter-associated urinary tract infections (CAUTIs), caused by biofilms, are the most frequent health-care associated infections. Novel antibiofilm coatings are needed to increase the urinary catheters' life-span, decrease the prevalence of CAUTIs and reduce the development of antimicrobial resistance. Herein, antibacterial zinc oxide nanoparticles (ZnO NPs) were decorated with a biofilm matrix-degrading enzyme amylase (AM) and simultaneously deposited onto silicone urinary catheters in a one-step sonochemical process. The obtained nano-enabled coatings inhibited the biofilm formation of <em>Escherichia coli</em> and <em>Staphylococcus aureus</em> by 80% and 60%, respectively, for up to 7 days <em>in vitro</em> in a model of catheterized bladder with recirculation of artificial urine due to the complementary mode of antibacterial and antibiofilm action provided by the NPs and the enzyme. Over this period, the coatings did not induce toxicity to mammalian cell lines. <em>In vivo</em>, the nano-engineered ZnO@AM coated catheters demonstrated lower incidence of bacteriuria and prevent the early onset of CAUTIs in a rabbit model, compared to the animals treated with pristine silicone devices. The nano-functionalization of catheters with hybrid ZnO@AM coatings appears as a promising strategy for prevention and control of CAUTIs in the clinic.</p></div>","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":"131 ","pages":"Article 112518"},"PeriodicalIF":7.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0928493121006585/pdfft?md5=225db294bb2916d9322b962b4f0aae91&pid=1-s2.0-S0928493121006585-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39687226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dopamine-assisted co-deposition of hydroxyapatite-functionalised nanoparticles of polydopamine on implant surfaces to promote osteogenesis in environments with high ROS levels","authors":"Yilin Yu ,&nbsp;Xiaolei Li ,&nbsp;Jiarun Li ,&nbsp;Dongying Li ,&nbsp;Qinmei Wang ,&nbsp;Wei Teng","doi":"10.1016/j.msec.2021.112473","DOIUrl":"10.1016/j.msec.2021.112473","url":null,"abstract":"<div><p>Environments with high reactive oxygen species (ROS) levels, which are common in patients with diseases such as diabetes, periodontitis, and osteoporosis, impair the osseointegration of implants. To address this issue, by using a one-pot dopamine-assisted co-deposition method, we constructed a three-dimensional coating of hydroxyapatite-functionalised nanoparticles of polydopamine (HA/nPDAs) on implant surfaces, where polydopamine is designed to protect cells via scavenging excessive ROS and HA facilitates osteogenesis. First, nanoparticles of polydopamine (nPDAs) were prepared by self-polymerization and assembly of dopamine under alkaline conditions, and HA/nPDAs were obtained by incubating nPDAs in simulated body fluid (SBF) due to metal chelation and ionic interactions triggered by the catechol moieties of PDA. Thereafter, HA/nPDAs with thickness of ~4 μm were constructed on titanium surfaces by immersing titanium discs in a weak alkaline solution of HA/nPDAs and dopamine through interface interactions driven by catechol chemistry. The properties of coatings (e.g., thickness, composition, hydrophilia and morphology) can be controlled by preparation conditions such as mineralization time and reactant concentration. The coatings display efficient ROS-scavenging ability, promote cell proliferation, and upregulate the activity of alkaline phosphatase and the expression of osteogenesis-related genes in environments with high or normal ROS levels, demonstrating the great promise of such coatings for osseointegration promotion, especially in the state of high ROS in diseases. This study provides a facile, efficient, mild, and universal strategy in engineering functional surfaces on any substrates for diversified applications by simple variation of co-deposited components, through taking advantages of versatile catechol chemistry and nanoparticles with stereo structure and great specific surface area.</p></div>","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":"131 ","pages":"Article 112473"},"PeriodicalIF":7.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0928493121006135/pdfft?md5=a75b3eab2d1ebbd91d2ad61a118e27b6&pid=1-s2.0-S0928493121006135-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39799090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D-printed porous Ti6Al4V alloys with silver coating combine osteocompatibility and antimicrobial properties.","authors":"A. Diez-Escudero, B. Andersson, Elin Carlsson, Benjamin Recker, H. Link, J. Järhult, N. Hailer","doi":"10.1016/j.msec.2021.112629","DOIUrl":"https://doi.org/10.1016/j.msec.2021.112629","url":null,"abstract":"","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":"40 1","pages":"112629"},"PeriodicalIF":7.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78418222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Double-responsive hyaluronic acid-based prodrugs for efficient tumour targeting","authors":"Vincenzo Quagliariello ,&nbsp;Arianna Gennari ,&nbsp;Som Akshay Jain ,&nbsp;Francesco Rosso ,&nbsp;Rosario Vincenzo Iaffaioli ,&nbsp;Alfonso Barbarisi ,&nbsp;Manlio Barbarisi ,&nbsp;Nicola Tirelli","doi":"10.1016/j.msec.2021.112475","DOIUrl":"10.1016/j.msec.2021.112475","url":null,"abstract":"<div><p>Hyaluronic acid (HA)-based prodrugs bearing double-responsive (acid pH or oxidation) boronates of catechol-containing drugs were used to treat xenografted human prostate tumours (LNCaP) in SCID mice. The HA prodrugs accumulated significantly only in tumours (impressively, up to 40% of the injected dose after 24 h) and in liver, with negligible – actually anti-inflammatory - consequences in the latter. A quercetin-HA prodrug significantly slowed down tumour growth, in a dose-dependent fashion and with a much higher efficacy (up to 4 times) than equivalent doses of free quercetin. In short, boronated HA appears to be a very promising platform for targeted chemotherapy.</p></div>","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":"131 ","pages":"Article 112475"},"PeriodicalIF":7.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0928493121006159/pdfft?md5=6c6310230d5c4577ead76049f7bd87b9&pid=1-s2.0-S0928493121006159-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39686025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peptide-modified substrate enhances cell migration and migrasome formation","authors":"Shogo Saito ,&nbsp;Masayoshi Tanaka ,&nbsp;Soichiro Tatematsu,&nbsp;Mina Okochi","doi":"10.1016/j.msec.2021.112495","DOIUrl":"10.1016/j.msec.2021.112495","url":null,"abstract":"<div><p>Extracellular vesicles (EVs) are cell-to-cell communication tools. Migrasomes are recently discovered microscale EVs formed at the rear ends of migrating cells, and thus are suggested to be involved in communicating with neighboring cells. In cell culture, peptide scaffolds on substrates have been used to demonstrate cellular function for regenerative medicine. In this study, we evaluated peptide scaffolds, including cell penetrating, virus fusion, and integrin-binding peptides, for their potential to enable the formation of migrasome-like vesicles. Through structural and functional analyses, we confirmed that the EVs formed on these peptide-modified substrates were migrasomes. We further noted that the peptide interface comprising cell-penetrating peptides (pVEC and R9) and virus fusion peptide (SIV) have superior properties for enabling cell migration and migrasome formation than fibronectin protein, integrin-binding peptide (RGD), or bare substrate. This is the first report of migrasome formation on peptide-modified substrates. Additionally, the combination of 95% RGD and 5% pVEC peptides provided a functional interface for effective migrasome formation and desorption of cells from the substrate via a simple ethylenediaminetetraacetic acid treatment. These results provide a functional substrate for the enhancement of migrasome formation and functional analysis.</p></div>","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":"131 ","pages":"Article 112495"},"PeriodicalIF":7.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0928493121006354/pdfft?md5=fa0de800491b97d7bc0506845cc6afb4&pid=1-s2.0-S0928493121006354-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39775289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical and biological properties of carboxymethyl chitosan zinc (CMCS-Zn)/α‑calcium sulfate hemihydrate (α-CSH) composites","authors":"Jianchun Lin ,&nbsp;Daorong Xu ,&nbsp;Zhongguo Liu ,&nbsp;Yi Jiang ,&nbsp;Mingliang Ren ,&nbsp;Haibo Xiang ,&nbsp;Bin Yu","doi":"10.1016/j.msec.2021.112496","DOIUrl":"10.1016/j.msec.2021.112496","url":null,"abstract":"<div><p>To improve the osteoinductivity, antibacterial activity, and clinical application of calcium sulfate hemihydrate (CSH), carboxymethyl chitosan zinc (CMCS-Zn) and α-CSH were prepared using different mass ratios. The setting time and injectability of the CMCS-Zn/α-CSH composite were increased with increasing CMCS-Zn content. After adding different amounts of CMCS-Zn to α-CSH, the fine lamellar structure of CMCS-Zn was found by scanning electron microscopy (SEM), which is evenly distributed in the matrix of α-CSH. With the increase of CMCS-Zn, the pores on the surface gradually increased. After mixing CMCS-Zn and α-CSH, no new phase was measured by X-ray diffraction (XRD) and Fourier transform (FTIR) spectroscopy. The degradation rate of CMCS-Zn/α-CSH decreased with increasing CMCS-Zn content, and the pH was stable during the degradation process. The release of Zn²⁺ increased with increasing CMCS-Zn content, while the release of Ca²⁺ decreased. Extracts of CMCS-Zn/α-CSH composites up-regulated the osteoinduction and migration of rat bone marrow stem cells. The antibacterial ability of CMCS-Zn/α-CSH was evaluated as a function of CMCS-Zn content. In the rat bone defect model, 5% CMCS-Zn/α-CSH group revealed a higher volume and density of trabeculae by micro-CT 8 weeks after the operation. Therefore, CMCS-Zn/α-CSH was demonstrated to be an adjustable, degradable, substitute biomaterial (with osteogenesis-promoting effects) for use in bone defects, which also has antibacterial activity that can suppress bone infection.</p></div>","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":"131 ","pages":"Article 112496"},"PeriodicalIF":7.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0928493121006366/pdfft?md5=52d0caed17c467653bf5f29a34833610&pid=1-s2.0-S0928493121006366-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39775290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanical properties, in vitro and in vivo biocompatibility analysis of pure iron porous implant produced by metal injection molding: A new eco-friendly feedstock from natural rubber (Hevea brasiliensis)","authors":"Diego Pacheco Wermuth ,&nbsp;Thaís Casagrande Paim ,&nbsp;Isadora Bertaco ,&nbsp;Carla Zanatelli ,&nbsp;Liliana Ivet Sous Naasani ,&nbsp;Mônica Slaviero ,&nbsp;David Driemeier ,&nbsp;André Carvalho Tavares ,&nbsp;Vinicius Martins ,&nbsp;Camila Ferreira Escobar ,&nbsp;Luis Alberto Loureiro dos Santos ,&nbsp;Lirio Schaeffer ,&nbsp;Márcia Rosângela Wink","doi":"10.1016/j.msec.2021.112532","DOIUrl":"10.1016/j.msec.2021.112532","url":null,"abstract":"<div><p>Metal injection molding (MIM) has become an important manufacturing technology for biodegradable medical devices. As a biodegradable metal, pure iron is a promising biomaterial due to its mechanical properties and biocompatibility. In light of this, we performed the first study that manufactured and evaluated the <em>in vitro</em> and <em>in vivo</em> biocompatibility of samples of iron porous implants produced by MIM with a new eco-friendly feedstock from natural rubber (<em>Hevea brasiliensis</em>), a promisor binder that provides elastic property in the green parts. The iron samples were submitted to tests to determine density, microhardness, hardness, yield strength, and stretching. The biocompatibility of the samples was studied <em>in vitro</em> with adipose-derived mesenchymal stromal cells (ADSCs) and erythrocytes, and <em>in vivo</em> on a preclinical model with Wistar rats, testing the iron samples after subcutaneous implant. Results showed that the manufactured samples have adequate physical, and mechanical characteristics to biomedical devices and they are cytocompatible with ADSCs, hemocompatible and biocompatible with Wistars rats. Therefore, pure iron produced by MIM can be considered a promising material for biomedical applications.</p></div>","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":"131 ","pages":"Article 112532"},"PeriodicalIF":7.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S092849312100672X/pdfft?md5=10dbb5eeff57c3eebfc27ae57978b715&pid=1-s2.0-S092849312100672X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39799101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}