Materials science & engineering. C, Materials for biological applications最新文献

{"title":"On-demand pulling-off of magnetic nanoparticles from biomaterial surfaces through implant-associated infectious biofilms for enhanced antibiotic efficacy","authors":"Kecheng Quan ,&nbsp;Zexin Zhang ,&nbsp;Yijin Ren ,&nbsp;Henk J. Busscher ,&nbsp;Henny C. van der Mei ,&nbsp;Brandon W. Peterson","doi":"10.1016/j.msec.2021.112526","DOIUrl":"10.1016/j.msec.2021.112526","url":null,"abstract":"<div><p>Biomaterial-associated infections can occur any time after surgical implantation of biomaterial implants and limit their success rates. On-demand, antimicrobial release coatings have been designed, but in vivo release triggers uniquely relating with infection do not exist, while inadvertent leakage of antimicrobials can cause exhaustion of a coating prior to need. Here, we attach magnetic-nanoparticles to a biomaterial surface, that can be pulled-off in a magnetic field through an adhering, infectious biofilm. Magnetic-nanoparticles remained stably attached to a surface upon exposure to PBS for at least 50 days, did not promote bacterial adhesion or negatively affect interaction with adhering tissue cells. Nanoparticles could be magnetically pulled-off from a surface through an adhering biofilm, creating artificial water channels in the biofilm. At a magnetic-nanoparticle coating concentration of 0.64 mg cm⁻², these by-pass channels increased the penetrability of <em>Staphylococcus aureus</em> and <em>Pseudomonas aeruginosa</em> biofilms towards different antibiotics, yielding 10-fold more antibiotic killing of biofilm inhabitants than in absence of artificial channels. This innovative use of magnetic-nanoparticles for the eradication of biomaterial-associated infections requires no precise targeting of magnetic-nanoparticles and allows more effective use of existing antibiotics by breaking the penetration barrier of an infectious biofilm adhering to a biomaterial implant surface on-demand.</p></div>","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":null,"pages":null},"PeriodicalIF":7.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0928493121006664/pdfft?md5=44bf8854c9b0b417af11235bfcd3d0e2&pid=1-s2.0-S0928493121006664-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39799096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Customized construction of microscale multi-component biostructures for cellular applications.","authors":"Zhixing Ge, Haibo Yu, Wenguang Yang, X. Liao, Xiaoduo Wang, P. Zhou, Jia Yang, Bin Liu, Lianqing Liu","doi":"10.1016/j.msec.2021.112599","DOIUrl":"https://doi.org/10.1016/j.msec.2021.112599","url":null,"abstract":"","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":null,"pages":null},"PeriodicalIF":7.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85425281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Preparation and characterization of withaferin A loaded pegylated nanoliposomal formulation with high loading efficacy: In vitro and in vivo anti-tumor study” [Mater. Sci. Eng. C 128 (2021) 112335]","authors":"Prathapan Abeesh,&nbsp;Walsan Kalarikkal Vishnu,&nbsp;Chandrasekharan Guruvayoorappan","doi":"10.1016/j.msec.2021.112432","DOIUrl":"10.1016/j.msec.2021.112432","url":null,"abstract":"","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":null,"pages":null},"PeriodicalIF":7.9,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0928493121005725/pdfft?md5=49b8affa403f9ebcf03f2ee8fa0f23b0&pid=1-s2.0-S0928493121005725-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39560182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pioneering personalised design of femoropopliteal nitinol stents","authors":"Ran He ,&nbsp;Liguo Zhao ,&nbsp;Vadim V. Silberschmidt ,&nbsp;Helen Willcock ,&nbsp;Felix Vogt","doi":"10.1016/j.msec.2021.112462","DOIUrl":"10.1016/j.msec.2021.112462","url":null,"abstract":"<div><h3>Background/motivation</h3><p>Percutaneous femoropopliteal artery intervention moves towards personalised therapy, which requires design of unique lesion-specific stents. However, to date, not much progress has been made in the development of personalised stents.</p></div><div><h3>Objective</h3><p>This paper aims to design personalised nitinol stents for femoropopliteal arteries based on medical imaging of patients and advanced computational mechanics, which is the first attempt to the authors' best knowledge.</p></div><div><h3>Methods</h3><p>The design process is based on three objectives: (i) achieving the healthy lumen area; (ii) reducing the stress in the media layer; (iii) improving the lumen shape after stenting. The design parameters include the strut width and thickness, the crown length, the nominal radius and the number of strut units per crown. Using representative unit-cell models, the effects of the five geometric parameters on the stent performance are investigated thoroughly with numerical simulations. Then, design protocols, especially for the circumferentially varying strut size and the oval stent shape, are developed and fully evaluated for an asymmetric stenosis.</p></div><div><h3>Results</h3><p>Using the design protocols, full personalised stents are designed for arteries with diffuse and focal plaques, based on medical imaging of patients. The personalised stent designs provide a double lumen gain, a reduced stress in the media layer and an improved lumen shape compared to a commercial stent.</p></div><div><h3>Conclusions</h3><p>The suggested protocols prove their high effectiveness in design of personalised stents, and the suggested approach can be applied to development of personalised therapies involving the use of stent technology including percutaneous coronary artery intervention, transcatheter aortic valve implantation, endovascular aneurysm repair and ureteric stenting.</p></div>","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":null,"pages":null},"PeriodicalIF":7.9,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0928493121006020/pdfft?md5=2e2965be8f90d6fe9c2c8922c5d4b5c8&pid=1-s2.0-S0928493121006020-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39561539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of sterilization treatments on biodegradability and cytocompatibility of zinc-based implant materials","authors":"Ping Li ,&nbsp;Wentai Zhang ,&nbsp;Sebastian Spintzyk ,&nbsp;Ernst Schweizer ,&nbsp;Stefanie Krajewski ,&nbsp;Dorothea Alexander ,&nbsp;Jingtao Dai ,&nbsp;Shulan Xu ,&nbsp;Guojiang Wan ,&nbsp;Frank Rupp","doi":"10.1016/j.msec.2021.112430","DOIUrl":"10.1016/j.msec.2021.112430","url":null,"abstract":"<div><p>Biodegradable zinc (Zn) and Zn-based alloys have been recognized as promising biomaterials for biomedical implants. Sterilization is an essential step in handling Zn-based implants before their use in clinical practice and there are various sterilization methods are available. However, how these treatments influence the Zn-based biomaterials remains unknown and is of critical relevance. In this study, three commonly-applied standard sterilization methods, namely gamma irradiation, hydrogen peroxide gas plasma and steam autoclave, were used on pure Zn and Zn<img>3Cu (wt%) alloy. The treated Zn and Zn<img>Cu alloy were investigated to compare the different influences of sterilizations on surface characteristics, transient and long-term degradation behavior and cytotoxicity of Zn and Zn alloy. Our results indicate that autoclaving brought about apparently a formation of inhomogeneous zinc oxide film whereas the other two methods produced no apparent alterations on the material surfaces. Consequently, the samples after autoclaving showed significantly faster degradation rates and more severe localized corrosion, especially for the Zn<img>Cu alloy, owing to the incomplete covering and unstable zinc oxide layer. Moreover, the autoclave-treated Zn and Zn<img>Cu alloy exhibited apparent cytotoxic effects towards fibroblasts, which may be due to the excessive Zn ion releasing and its local concentration exceeds the cellular tolerance capacity. In contrast, gamma irradiation and hydrogen peroxide gas plasma had no apparent adverse effects on the biodegradability and cytocompatibility of Zn and Zn<img>Cu alloy. Our findings may have significant implications regarding the selection of suitable sterilization methods for Zn-based implant materials among others.</p></div>","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":null,"pages":null},"PeriodicalIF":7.9,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0928493121005701/pdfft?md5=7946000faefd33c098eb7410c252c5db&pid=1-s2.0-S0928493121005701-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39560180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ascorbic acid-loaded polyvinyl alcohol/cellulose nanofibril hydrogels as precursors for 3D printed materials","authors":"Hossein Baniasadi ,&nbsp;Zahraalsadat Madani ,&nbsp;Rubina Ajdary ,&nbsp;Orlando J. Rojas ,&nbsp;Jukka Seppälä","doi":"10.1016/j.msec.2021.112424","DOIUrl":"10.1016/j.msec.2021.112424","url":null,"abstract":"<div><p>We proposed a simple method to process hydrogels containing polyvinyl alcohol and cellulose nanofibrils (PVA/CNF) to prepare volumetric architectures by direct ink writing (DIW). The presence of CNF in the aqueous PVA suspensions conferred rheology profiles that were suitable for extrusion and solidification in pre-designed shapes. The viscoelastic behavior of the hybrid inks enabled precise control on processability and shape retention, for instance, as demonstrated in multilayered lattice structures of high fidelity. After lyophilization, the obtained 3D-printed hydrogels presented a very high porosity, with open and interconnected pores, allowing a high-water uptake capacity (up to 1600%). The mechanical strength of the composite 3D-printed materials matched those of soft tissues, opening opportunities for skin applications. As such, drug-loaded samples revealed a controlled and efficient delivery of an antioxidant (ascorbic acid) in PBS buffer media at 23 °C (~80% for 8 h). Altogether, PVA/CNF hydrogels were introduced as suitable precursors of 3D-lattice geometries with excellent physical and mechanical characteristics.</p></div>","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":null,"pages":null},"PeriodicalIF":7.9,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.msec.2021.112424","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39560293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “3D-printed Mg-incorporated PCL-based scaffolds: A promising approach for bone healing” [Mater. Sci. Eng. C 129 (2021) 112372]","authors":"Qiangsheng Dong ,&nbsp;Ming Zhang ,&nbsp;Xingxing Zhou ,&nbsp;Yi Shao ,&nbsp;Jiayi Li ,&nbsp;Liming Wang ,&nbsp;Chenglin Chu ,&nbsp;Feng Xue ,&nbsp;Qingqiang Yao ,&nbsp;Jing Bai","doi":"10.1016/j.msec.2021.112443","DOIUrl":"10.1016/j.msec.2021.112443","url":null,"abstract":"","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":null,"pages":null},"PeriodicalIF":7.9,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S092849312100583X/pdfft?md5=3ebebc864473e64c7c877755202d9a39&pid=1-s2.0-S092849312100583X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39563532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The surface modification of long carbon fiber reinforced polyether ether ketone with bioactive composite hydrogel for effective osteogenicity","authors":"Wenying Dong ,&nbsp;Wendi Ma ,&nbsp;Shanshan Zhao ,&nbsp;Yilong Wang ,&nbsp;Jihang Yao ,&nbsp;Zhewen Liu ,&nbsp;Zheng Chen ,&nbsp;Dahui Sun ,&nbsp;Zhenhua Jiang ,&nbsp;Mei Zhang","doi":"10.1016/j.msec.2021.112451","DOIUrl":"10.1016/j.msec.2021.112451","url":null,"abstract":"<div><p>Long carbon fiber reinforced polyether ether ketone (LCFRPEEK) is fabricated using a three-dimensional (3D) needle-punched method in our previous work, which is considered as a potential orthopedic implant due to its high mechanical strength and isotropic properties, as well as having an elastic modulus similar to human cortical bone. However, the LCFRPEEK has inferior integration with bone tissue, limiting its clinical application. Thus, a facile surface modification method, using gelatin methacrylate/polyacrylamide composite hydrogel coating (GelMA/PAAM) loading with dexamethasone (Dex) on our newly-developed LCFRPEEK composite <em>via</em> concentrated sulfuric acid sulfonating and ultraviolet (UV) irradiation grafting methods, has been developed to tackle the problem. The results demonstrate that the GelMA/PAAM/Dex coating modified sulfonated LCFRPEEK (SCP/GP/Dex) has a hydrophilicity surface, a long-term Dex release capability and forms more bone-like apatite nodules in SBF. The SCP/GP/Dex also displays enhanced cytocompatibility and osteogenic differentiation in terms of rat bone marrow mesenchymal stem cells (rBMSCs) responses <em>in vitro</em> assay. The <em>in vivo</em> rat cranial defect assay confirms that SCP/GP/Dex boosts bone regeneration/osseointegration, which significantly improves osteogenic fixation between the implant and bone tissue. Therefore, the newly-developed LCFRPEEK modified <em>via</em> GelMA/PAAM/Dex bioactive coating exhibits improved biocompatibility and osteogenic integration capability, which has the basis for an orthopedic implant for clinical application.</p></div>","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":null,"pages":null},"PeriodicalIF":7.9,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0928493121005919/pdfft?md5=819ec54195c79748852f0fe8540fb5c3&pid=1-s2.0-S0928493121005919-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39563534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a novel direct dental pulp-capping material using 4-META/MMA-TBB resin with nano hydroxyapatite","authors":"Shinichiro Yoshida ,&nbsp;Hideki Sugii ,&nbsp;Tomohiro Itoyama ,&nbsp;Masataka Kadowaki ,&nbsp;Daigaku Hasegawa ,&nbsp;Atsushi Tomokiyo ,&nbsp;Sayuri Hamano ,&nbsp;Keita Ipposhi ,&nbsp;Kozue Yamashita ,&nbsp;Hidefumi Maeda","doi":"10.1016/j.msec.2021.112426","DOIUrl":"10.1016/j.msec.2021.112426","url":null,"abstract":"<div><p>In the case of dental pulp exposure, direct pulp capping is often performed to preserve vital dental pulp tissue. Numerous studies regarding the development of direct pulp-capping materials have been conducted, but materials with an appropriate sealing ability, which induce dense reparative dentin formation, have not been developed. Although nano hydroxyapatite (naHAp) is a bone-filling material with bioactivity and biocompatibility, the inductive effects of naHAp on reparative dentin formation remain unclear. In the present study, the effects of dental adhesive material 4-methacryloxyethyl trimellitate anhydride/methyl methacrylate tri-n-butylborane [4-META/MMA-TBB or Super-bond (SB)], which included 10%, 30%, and 50% naHAp (naHAp/SB) on odontoblastic differentiation of dental pulp stem cells (DPSCs) and reparative dentin formation were investigated. Scanning electron microscopy (SEM) and energy dispersive X-ray spectrometer analysis were performed to verify the existence of naHAp particles on the surface of naHAp/SB discs. The tensile adhesive strength of naHAp/SB was measured using a universal testing machine. As a result, 10% naHAp/SB and 30% naHAp/SB showed almost the same tensile adhesive strength as SB but 50% naHAp/SB showed significantly lower than the other experimental group. WST-1 proliferation assay and SEM analysis revealed that naHAp/SB did not affect the proliferation of DPSCs. Calcium release assay, quantitative RT-PCR, and western blotting analysis demonstrated that naHAp/SB did not release calcium ion but 30% naHAp/SB increased the expression of calcium-sensing receptor (CaSR) in DPSCs. Additionally, quantitative RT-PCR, western blotting analysis, Alizarin Red S- and von Kossa staining revealed that 30% naHAp/SB induced odontoblastic differentiation of DPSCs, which was inhibited by a MEK/ERK inhibitor and CaSR antagonist. Furthermore, 30% naHAp/SB promoted dense reparative dentin formation in an experimentally-formed rat dental pulp exposure model. These findings suggest that 30% naHAp/SB can be used as an ideal direct pulp capping material.</p></div>","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":null,"pages":null},"PeriodicalIF":7.9,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S092849312100566X/pdfft?md5=2e91bbcbbf7870f022018e0b33124640&pid=1-s2.0-S092849312100566X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39560176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Living Lactobacillus–ZnO nanoparticles hybrids as antimicrobial and antibiofilm coatings for wound dressing application","authors":"Fereshte Nazemi Harandi ,&nbsp;Alireza Chackoshian Khorasani ,&nbsp;Seyed Abbas Shojaosadati ,&nbsp;Sameereh Hashemi-Najafabadi","doi":"10.1016/j.msec.2021.112457","DOIUrl":"10.1016/j.msec.2021.112457","url":null,"abstract":"<div><p>Probiotic bacteria are able to produce antimicrobial substances as well as to synthesize green metal nanoparticles (NPs). New antimicrobial and antibiofilm coatings (LAB-ZnO NPs), composed of <em>Lactobacillus</em> strains and green ZnO NPs, were employed for the modification of gum Arabic-polyvinyl alcohol-polycaprolactone nanofibers matrix (GA-PVA-PCL) against <em>Escherichia coli</em>, <em>Staphylococcus aureus</em>, <em>Pseudomonas aeruginosa</em> and <em>Candida albicans</em>. The physicochemical properties of ZnO NPs biologically synthesized by <em>L. plantarum</em> and <em>L. acidophilus</em>, LAB-ZnO NPs hybrids and LAB-ZnO NPs@GA-PVA-PCL were studied using FE-SEM, EDX, EM, FTIR, XRD and ICP-OES. The morphology of LAB-ZnO NPs hybrids was spherical in range of 4.56–91.61 nm with an average diameter about 34 nm. The electrospun GA-PVA-PCL had regular, continuous and without beads morphology in the scale of nanometer and micrometer with an average diameter of 565 nm. Interestingly, the LAB not only acted as a biosynthesizer in the green synthesis of ZnO NPs but also synergistically enhanced the antimicrobial and antibiofilm efficacy of LAB-ZnO NPs@GA-PVA-PCL. Moreover, the low cytotoxicity of ZnO NPs and ZnO NPs@GA-PVA-PCL on the mouse embryonic fibroblasts cell line led to make them biocompatible. These results suggest that LAB-ZnO NPs@GA-PVA-PCL has potential as a safe promising antimicrobial and antibiofilm dressing in wound healing against pathogens.</p></div>","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":null,"pages":null},"PeriodicalIF":7.9,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S092849312100597X/pdfft?md5=e4db6fa3e824f4e7ea69b775c850c032&pid=1-s2.0-S092849312100597X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39563537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}