灭菌处理对锌基种植材料生物降解性和细胞相容性的影响

IF 8.1 1区 工程技术 Q1 MATERIALS SCIENCE, BIOMATERIALS
Ping Li , Wentai Zhang , Sebastian Spintzyk , Ernst Schweizer , Stefanie Krajewski , Dorothea Alexander , Jingtao Dai , Shulan Xu , Guojiang Wan , Frank Rupp
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引用次数: 4

摘要

生物可降解锌(Zn)和锌基合金是一种很有前途的生物医学植入材料。在临床实践中使用锌基植入物之前,灭菌是处理锌基植入物的重要步骤,有各种灭菌方法可供选择。然而,这些治疗如何影响锌基生物材料仍然是未知的,这是至关重要的。本研究采用γ辐照、过氧化氢气体等离子体和蒸汽高压灭菌法对纯Zn和Zn3Cu (wt%)合金进行了三种常用的标准灭菌方法。研究了处理后的Zn和ZnCu合金,比较了灭菌对Zn和Zn合金表面特性、暂态和长期降解行为以及细胞毒性的不同影响。我们的结果表明,高压灭菌带来了明显的不均匀氧化锌膜的形成,而其他两种方法对材料表面没有明显的改变。因此,高压灭菌后的样品由于覆盖不完全,氧化锌层不稳定,其降解速度明显加快,局部腐蚀更加严重,尤其是ZnCu合金。此外,高压灭菌处理的Zn和ZnCu合金对成纤维细胞表现出明显的细胞毒性作用,这可能是由于锌离子释放过量,其局部浓度超过细胞耐受能力。伽马辐照和过氧化氢等离子体对Zn和ZnCu合金的生物降解性和细胞相容性没有明显的不利影响。我们的研究结果可能对选择合适的消毒方法的锌基种植材料等具有重要意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Impact of sterilization treatments on biodegradability and cytocompatibility of zinc-based implant materials

Biodegradable zinc (Zn) and Zn-based alloys have been recognized as promising biomaterials for biomedical implants. Sterilization is an essential step in handling Zn-based implants before their use in clinical practice and there are various sterilization methods are available. However, how these treatments influence the Zn-based biomaterials remains unknown and is of critical relevance. In this study, three commonly-applied standard sterilization methods, namely gamma irradiation, hydrogen peroxide gas plasma and steam autoclave, were used on pure Zn and Zn3Cu (wt%) alloy. The treated Zn and ZnCu alloy were investigated to compare the different influences of sterilizations on surface characteristics, transient and long-term degradation behavior and cytotoxicity of Zn and Zn alloy. Our results indicate that autoclaving brought about apparently a formation of inhomogeneous zinc oxide film whereas the other two methods produced no apparent alterations on the material surfaces. Consequently, the samples after autoclaving showed significantly faster degradation rates and more severe localized corrosion, especially for the ZnCu alloy, owing to the incomplete covering and unstable zinc oxide layer. Moreover, the autoclave-treated Zn and ZnCu alloy exhibited apparent cytotoxic effects towards fibroblasts, which may be due to the excessive Zn ion releasing and its local concentration exceeds the cellular tolerance capacity. In contrast, gamma irradiation and hydrogen peroxide gas plasma had no apparent adverse effects on the biodegradability and cytocompatibility of Zn and ZnCu alloy. Our findings may have significant implications regarding the selection of suitable sterilization methods for Zn-based implant materials among others.

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来源期刊
CiteScore
12.60
自引率
0.00%
发文量
28
审稿时长
3.3 months
期刊介绍: Materials Today is a community committed to fostering the creation and sharing of knowledge and experience in materials science. With the support of Elsevier, this community publishes high-impact peer-reviewed journals, organizes academic conferences, and conducts educational webinars, among other initiatives. It serves as a hub for advancing materials science and facilitating collaboration within the scientific community.
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