Materials science & engineering. C, Materials for biological applications最新文献

{"title":"Preparation of pro-angiogenic, antibacterial and EGCG-modified ZnO quantum dots for treating bacterial infected wound of diabetic rats.","authors":"X. Yin, Shuocheng Huang, Shibo Xu, Linna Chang, Xingjun Zhao, Zhenhua Chen, Xifan Mei, Xiuqiu Gao","doi":"10.1016/j.msec.2021.112638","DOIUrl":"https://doi.org/10.1016/j.msec.2021.112638","url":null,"abstract":"","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":"56 1","pages":"112638"},"PeriodicalIF":7.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88527101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Orai1 mediated store-operated calcium entry contributing to MC3T3-E1 differentiation on titanium implant with micro/nano-textured topography.","authors":"Guangwen Li, Bei Chang, Yide He, Yi Li, Jing Liu, Y. Zhang, Yajie Hou, Boya Xu, Xinyan Li, Min Xu, Xin Ding, Wen Song, Yumei Zhang","doi":"10.1016/j.msec.2022.112644","DOIUrl":"https://doi.org/10.1016/j.msec.2022.112644","url":null,"abstract":"","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":"4 1","pages":"112644"},"PeriodicalIF":7.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81854559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neural stem cell-laden 3D bioprinting of polyphenol-doped electroconductive hydrogel scaffolds for enhanced neuronal differentiation.","authors":"Shaoshuai Song, Xiaoyun Liu, Jie Huang, Zhijun Zhang","doi":"10.1016/j.msec.2021.112639","DOIUrl":"https://doi.org/10.1016/j.msec.2021.112639","url":null,"abstract":"","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":"69 1","pages":"112639"},"PeriodicalIF":7.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86909195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autologous stromal vascular fraction-loaded hyaluronic acid/gelatin-biphasic calcium phosphate scaffold for bone tissue regeneration","authors":"Seong-su Park ,&nbsp;Myeongki Park ,&nbsp;Byong-Taek Lee","doi":"10.1016/j.msec.2021.112533","DOIUrl":"10.1016/j.msec.2021.112533","url":null,"abstract":"<div><p>Bone defect augmentation with synthetic materials is crucial due to the unavoidable limitations of auto- and allografting. Although there are different promising synthetic materials for filling bone defects, the functionalization of these materials with cells is still challenging due to the lack of ideal cell sources. Here, we used stromal vascular fraction (SVF) heterogeneous cells that could be obtained from autologous adipose tissue to functionalize hyaluronic acid/gelatin-biphasic calcium phosphate (HyA-Gel/BCP) scaffolds for bone regeneration. The SVF cells were isolated, and the cellular composition and osteogenic differentiation potential were analyzed. Then, they were cultured on HyA-Gel/BCP scaffolds for <em>in vitro</em> characterization. An <em>In vivo</em> evaluation of the autologous SVF-loaded HyA-Gel/BCP scaffolds was performed using a rat skull critical-size defect model. The results showed that the SVF was successfully isolated and contained different types of cells, including mesenchymal stem like-cells with osteogenic differentiation ability. Also, the SVF cells could be cultured and expanded on the HyA-Gel/BCP scaffolds without affecting their viability. <em>In vivo</em> implantation of autologous SVF-loaded HyA-Gel/BCP scaffolds showed excellent bone regeneration compared to unloaded HyA-Gel/BCP scaffolds. Thus, autologous SVF-loaded HyA-Gel/BCP scaffolds could be a promising transplantable bone grafting material for bone tissue engineering.</p></div>","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":"132 ","pages":"Article 112533"},"PeriodicalIF":7.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0928493121006731/pdfft?md5=45bf0becdd85c3b9910f1e929884aee3&pid=1-s2.0-S0928493121006731-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39772670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nature of bilayer lipids affects membranes deformation and pore resealing during nanoparticle penetration","authors":"Yousef Nademi ,&nbsp;Tian Tang ,&nbsp;Hasan Uludağ","doi":"10.1016/j.msec.2021.112530","DOIUrl":"10.1016/j.msec.2021.112530","url":null,"abstract":"<div><p>Interactions of nanoparticles (NPs) with lipid membranes have enormous biological implications especially for gene delivery applications. In this work, using all-atom steered- and molecular dynamics simulations, we investigated deformation of lipid membranes and pore closure during a NP penetration process. Three membrane bilayer models built from 2-oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine (POPC), dipalmitoylphosphatidylcholine (DPPC) and dilauroylphosphatidylcholine (DLPC), and a NP formed by 2 short interfering RNA (siRNA) and 6 polyethylenimine (PEI) molecules were used. Our results showed that different membrane lipids could lead to differences in pore formation (symmetric vs. asymmetric), and could undergo different levels of pore-mediated flip-flops during the closure. DLPC showed the largest number of flip-flops among the three lipid membranes. In addition, introduction of hydrophobic linoleic acid (LA) substitution onto the PEIs was found to facilitate pore formation, since the long LA tails could insert themselves into the hydrophobic region of the membrane where the lipid tails were less aligned. Compared with DPPC, POPC and DLPC membranes had less alignment of lipid tails in the bilayer, which promoted the insertion of LA tails and hence NP entry into the cell. Our observations provide valuable insight into the membrane deformations and pore dynamics during NP penetration and will be important for the design of NP carriers for effective gene delivery.</p></div>","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":"132 ","pages":"Article 112530"},"PeriodicalIF":7.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0928493121006706/pdfft?md5=a41d37fe92f827a0837c58d3adb0b270&pid=1-s2.0-S0928493121006706-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39910970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoparticles-stacked superhydrophilic coating supported synergistic antimicrobial ability for enhanced wound healing","authors":"Linhua Li ,&nbsp;Yanan Wang ,&nbsp;Kunpeng Liu ,&nbsp;Li Yang ,&nbsp;Bo Zhang ,&nbsp;Qingfeng Luo ,&nbsp;Rifang Luo ,&nbsp;Yunbing Wang","doi":"10.1016/j.msec.2021.112535","DOIUrl":"10.1016/j.msec.2021.112535","url":null,"abstract":"<div><p>Medical device infections have now become the major burden of healthcare, and particular administration of combating bacterial infections is of significance. In this work, robust nanoparticles-stacked superhydrophilic coatings were established through the rapid oxidation, cross-linking and aggregation of dopamine in the presence of sodium periodate. The robust superhydrophilicity was achieved and maintained due to the hydrophilic chemical components together with the micro/nano topological structure stacked by nanoparticles, resulting in an impressive nonfouling performance for proteins adsorption. Moreover, due to the presence of aromatic catechol moieties, antibiotics (e.g. norfloxacin and cephalexin) were deposited into the superhydrophilic coating in situ, by π-π stacking/hydrophobic interactions, endowing the surface with antibacterial ability. Interestingly, the superhydrophilic coatings showed a safe and effective antibacterial ability in a low dose-dependent manner because of the nonfouling platform supported killing and releasing of bacteria. The in vivo cutaneous wound healing evaluation in rats further demonstrated the synchronous effect of anti-infection and promoting wound healing. Such superhydrophilicity supported nonfouling platform was believed to open a new window to modify biomedical devices combined with wound healing and antibacterial properties.</p></div>","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":"132 ","pages":"Article 112535"},"PeriodicalIF":7.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0928493121006755/pdfft?md5=23efbad39eb7538ec97410fb1a9b0914&pid=1-s2.0-S0928493121006755-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39867646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Klotho functionalization on vascular graft for improved patency and endothelialization.","authors":"Pan Zhao, Q. Fang, Dongsheng Gao, Qiang Wang, Yanbin Cheng, Q. Ao, Xiaohong Wang, Xiaohong Tian, Yanhui Zhang, Hao Tong, N. Yan, Xinkang Hu, Jun Fan","doi":"10.1016/j.msec.2021.112630","DOIUrl":"https://doi.org/10.1016/j.msec.2021.112630","url":null,"abstract":"","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":"317 1","pages":"112630"},"PeriodicalIF":7.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75697348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ZnO-loaded DNA nanogels as neutrophil extracellular trap-like structures in the treatment of mouse peritonitis","authors":"Yu-Fon Chen ,&nbsp;Yee-Hsuan Chiou ,&nbsp;Yi-Cheng Chen ,&nbsp;Yi-Sheng Jiang ,&nbsp;Ting-Yuan Lee ,&nbsp;Jeng-Shiung Jan","doi":"10.1016/j.msec.2021.112484","DOIUrl":"10.1016/j.msec.2021.112484","url":null,"abstract":"<div><p>Neutrophil extracellular traps (NETs) are chromatin-based structures that are released from neutrophils during infections and prevent microbes from spreading in the body through efficient degradation of their composition. Based on this chromatin-driven strategy of capturing and killing bacteria, we designed NET-like structures using DNA and ZnO nanoparticles (NPs). DNA was first purified from kiwifruit and treated with HCl to increase hydroxyl groups in the opened-deoxylribose form. The carboxyl groups of citric acid were then thermally crosslinked with said hydroxyl and primary amine groups in DNA, forming DNA-HCl nanogels (NGs). ZnO NPs were then used as positively charged granule enzymes, adsorbed onto the DNA-HCl NG, obtaining ZnO/DNA-HCl NGs (with NET biomimicry). In an anti-inflammatory assay, ZnO/DNA-HCl NGs significantly inhibited TNF-α, IL-6, iNOS and COX-2 expression in LPS-stimulated Raw264.7 cells. Moreover, the ZnO/DNA-HCl NGs markedly alleviated clinical symptoms in LPS-induced mouse peritonitis. Finally, ZnO/DNA-HCl NGs suppressed <em>E. coli</em> from entering circulation in septic mice while prolonging their survival. Our results suggest that the ZnO/DNA-HCl NGs, which mimic NET-like structures in the blocking of bacteria-inducted inflammation, may be a potential therapeutic strategy for bacterial infections.</p></div>","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":"131 ","pages":"Article 112484"},"PeriodicalIF":7.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S092849312100624X/pdfft?md5=302ef6c49cd069a135339c69052ce543&pid=1-s2.0-S092849312100624X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39686031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mg-Fe layered double hydroxides modified titanium enhanced the adhesion of human gingival fibroblasts through regulation of local pH level","authors":"Yijia Yin ,&nbsp;Linjia Jian ,&nbsp;Baoe Li ,&nbsp;Chunyong Liang ,&nbsp;Xianglong Han ,&nbsp;Xuefeng Zhao ,&nbsp;Donghui Wang","doi":"10.1016/j.msec.2021.112485","DOIUrl":"10.1016/j.msec.2021.112485","url":null,"abstract":"<div><p>The durability of dental implants is closely related to osseointegration and surrounding soft tissue sealing. Appropriate local pH favors fibroblasts adhesion and contributes to soft tissue sealing. Layered double hydroxides (LDHs) are characterized by adjustable alkalinity, offering a possibility to investigate the influence of pH on cellular behaviors. Herein, we fabricated Mg<img>Fe LDHs modified titanium. During calcination, the local pH value of LDHs increase, without altering other physics and chemical properties via OH⁻ exchange mechanism. In vitro studies showed that LDHs films calcined at 250 °C for 2 h provide a local pH of 10.17, which promote early adhesion, proliferation, and type I collagen expression of human gingival fibroblasts (hGFs) through the formation of focal adhesion complex and activation of focal adhesion kinase related signaling pathways. In conclusion, endowing the titanium surface with appropriate alkalinity by Mg<img>Fe LDHs films enhances the adhesion of hGFs, providing a new strategy of designing multifunctional biomaterials for soft tissue sealing around dental implants.</p></div>","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":"131 ","pages":"Article 112485"},"PeriodicalIF":7.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0928493121006251/pdfft?md5=86d4d7d83df68f4a665221d5c5c607cc&pid=1-s2.0-S0928493121006251-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39686032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular matrix-inspired surface coatings functionalized with dexamethasone-loaded liposomes to induce osteo- and chondrogenic differentiation of multipotent stem cells","authors":"Yazmin A. Brito Barrera ,&nbsp;Catharina Husteden ,&nbsp;Jumanah Alherz ,&nbsp;Bodo Fuhrmann ,&nbsp;Christian Wölk ,&nbsp;Thomas Groth","doi":"10.1016/j.msec.2021.112516","DOIUrl":"10.1016/j.msec.2021.112516","url":null,"abstract":"<div><p>Biomimetic surface coatings can be combined with conventional implants to mimic the extracellular matrix (ECM) of the surrounding tissue to make them more biocompatible. Layer-by-layer technique (LbL) can be used for making surface coatings by alternating adsorption of polyanions and polycations from aqueous solutions without need of chemical reactions. Here, polyelectrolyte multilayer (PEM) systems is made of hyaluronic acid (HA) as polyanion and Collagen I (Col) as polycation to mimic the ECM of connective tissue. The PEM are combined with dexamethasone (Dex)-loaded liposomes to achieve a local delivery and protection of this drug for stimulation of osteo- and chondrogenic differentiation of multipotent stem cells. The liposomes possess a positive surface charge that is required for immobilization on the PEM. The surface properties of PEM system show a positive zeta potential after liposome adsorption and a decrease in wettability, both promoting cell adhesion and spreading of C3H10T1/2 multipotent embryonic mouse fibroblasts. Differentiation of C3H10T1/2 was more prominent on the PEM system with embedded Dex-loaded liposomes compared to the basal PEM system and the use of free Dex-loaded liposomes in the supernatant. This was evident by immunohistochemical staining and an upregulation of the expression of genes, which play a key role in osteogenesis (RunX2, ALP, Osteocalcin (OCN)) and chondrogenesis (Sox9, aggrecan (ACAN), collagen type II), determined by quantitative Real-time polymerase chain reaction (qRT-PCR) after 21 days. These findings indicate that the designed liposome-loaded PEM system have high potential for use as drug delivery systems for implant coatings that can induce bone and cartilage differentiation needed for example in osteochondral implants.</p></div>","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":"131 ","pages":"Article 112516"},"PeriodicalIF":7.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0928493121006561/pdfft?md5=07ee6db2ba7fc072e78f206020231ada&pid=1-s2.0-S0928493121006561-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39686245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}