Extracellular matrix-inspired surface coatings functionalized with dexamethasone-loaded liposomes to induce osteo- and chondrogenic differentiation of multipotent stem cells

IF 8.1 1区 工程技术 Q1 MATERIALS SCIENCE, BIOMATERIALS
Yazmin A. Brito Barrera , Catharina Husteden , Jumanah Alherz , Bodo Fuhrmann , Christian Wölk , Thomas Groth
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引用次数: 8

Abstract

Biomimetic surface coatings can be combined with conventional implants to mimic the extracellular matrix (ECM) of the surrounding tissue to make them more biocompatible. Layer-by-layer technique (LbL) can be used for making surface coatings by alternating adsorption of polyanions and polycations from aqueous solutions without need of chemical reactions. Here, polyelectrolyte multilayer (PEM) systems is made of hyaluronic acid (HA) as polyanion and Collagen I (Col) as polycation to mimic the ECM of connective tissue. The PEM are combined with dexamethasone (Dex)-loaded liposomes to achieve a local delivery and protection of this drug for stimulation of osteo- and chondrogenic differentiation of multipotent stem cells. The liposomes possess a positive surface charge that is required for immobilization on the PEM. The surface properties of PEM system show a positive zeta potential after liposome adsorption and a decrease in wettability, both promoting cell adhesion and spreading of C3H10T1/2 multipotent embryonic mouse fibroblasts. Differentiation of C3H10T1/2 was more prominent on the PEM system with embedded Dex-loaded liposomes compared to the basal PEM system and the use of free Dex-loaded liposomes in the supernatant. This was evident by immunohistochemical staining and an upregulation of the expression of genes, which play a key role in osteogenesis (RunX2, ALP, Osteocalcin (OCN)) and chondrogenesis (Sox9, aggrecan (ACAN), collagen type II), determined by quantitative Real-time polymerase chain reaction (qRT-PCR) after 21 days. These findings indicate that the designed liposome-loaded PEM system have high potential for use as drug delivery systems for implant coatings that can induce bone and cartilage differentiation needed for example in osteochondral implants.

Abstract Image

细胞外基质激发表面涂层与地塞米松装载脂质体功能化诱导多能干细胞成骨和软骨分化
仿生表面涂层可以与传统植入物结合,模拟周围组织的细胞外基质(ECM),使其更具生物相容性。逐层技术(LbL)可以通过交替吸附水溶液中的聚阴离子和聚阳离子来制备表面涂层,而不需要化学反应。在这里,聚电解质多层(PEM)系统由透明质酸(HA)作为聚阴离子和胶原I (Col)作为聚阳离子组成,以模拟结缔组织的ECM。PEM与装载地塞米松(Dex)的脂质体结合,实现药物的局部递送和保护,以刺激多能干细胞的成骨和软骨分化。脂质体具有固定在PEM上所需的正表面电荷。PEM系统的表面特性在脂质体吸附后呈现正的zeta电位,润湿性降低,促进了C3H10T1/2多能胚胎小鼠成纤维细胞的粘附和扩散。与基础PEM体系和在上清液中使用游离dex脂质体相比,在内含dex脂质体的PEM体系中,C3H10T1/2的分化更为突出。通过免疫组化染色和21天后定量实时聚合酶链反应(qRT-PCR)检测的在成骨(RunX2、ALP、骨钙素(OCN))和软骨形成(Sox9、聚集蛋白(ACAN)、II型胶原)中起关键作用的基因表达上调,可以证明这一点。这些发现表明,所设计的脂质体负载PEM系统具有很高的潜力,可以作为药物递送系统用于植入物涂层,可以诱导骨和软骨分化,例如骨软骨植入物。
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来源期刊
CiteScore
12.60
自引率
0.00%
发文量
28
审稿时长
3.3 months
期刊介绍: Materials Today is a community committed to fostering the creation and sharing of knowledge and experience in materials science. With the support of Elsevier, this community publishes high-impact peer-reviewed journals, organizes academic conferences, and conducts educational webinars, among other initiatives. It serves as a hub for advancing materials science and facilitating collaboration within the scientific community.
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