Magnesium research最新文献

{"title":"The assessment of intracellular magnesium: different strategies to answer different questions.","authors":"Giovanna Picone,&nbsp;Concettina Cappadone,&nbsp;Giovanna Farruggia,&nbsp;Emil Malucelli,&nbsp;Stefano Iotti","doi":"10.1684/mrh.2020.0464","DOIUrl":"https://doi.org/10.1684/mrh.2020.0464","url":null,"abstract":"<p><p>The role of magnesium in cell metabolism is complex and still not completely clarified. Although magnesium has been shown to modulate many phenomena in cells, its intracellular distribution and subcellular compartmentalization have not yet elucidated in detail, mainly as a consequence of the inadequacy of analytical techniques. The method usually employed to quantify total magnesium in cells or tissue are F-AAS or more sensitive techniques as graphite furnace AAS and inductively coupled plasma mass spectroscopy (MS). Thanks to the development of new specific fluorescent dyes, several progresses have been made in the comprehension of the fundamental biological process at the cellular and sub-cellular level. Moreover, the biological function of a chemical element in cells does not only require the determination of its intracellular quantity but also the spatial distribution of its concentration. Most of Mg²⁺-sensitive fluorescent dyes detect only the free metal ions, precluding the possibility of identifying the total pool of Mg. This review aims at giving an overview on different techniques focusing on two approaches to quantify total Mg in a small cell population or in single cells: i) Indirect Mg detection, label-based methods that represent the best choice to quantify the elemental concentration on a large cell population; ii) direct Mg detection (label-free), Synchrotron-based x-ray microscopy techniques that offer the possibility of achieving a detailed map of the intracellular concentration of a specific chemical element on single cell.</p>","PeriodicalId":18159,"journal":{"name":"Magnesium research","volume":"33 1","pages":"1-11"},"PeriodicalIF":3.2,"publicationDate":"2020-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38132871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The permeability of magnesium across the skin is enhanced by menthol cream","authors":"Veera Turunen, S. Peräniemi, K. Tolvanen, M. Kokki, Marika Ruponen, H. Kokki","doi":"10.1684/mrh.2020.0460","DOIUrl":"https://doi.org/10.1684/mrh.2020.0460","url":null,"abstract":"","PeriodicalId":18159,"journal":{"name":"Magnesium research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1684/mrh.2020.0460","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48626545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biopharmaceutics classification and pharmacokinetics study of magnesium orotate","authors":"H. Verma, R. Garg","doi":"10.1684/mrh.2020.0462","DOIUrl":"https://doi.org/10.1684/mrh.2020.0462","url":null,"abstract":"","PeriodicalId":18159,"journal":{"name":"Magnesium research","volume":"1 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1684/mrh.2020.0462","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41520432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk factors of hypomagnesemia in hospitalized patients with end-stage cancer","authors":"Masahiro Takahashi","doi":"10.1684/mrh.2020.0461","DOIUrl":"https://doi.org/10.1684/mrh.2020.0461","url":null,"abstract":"","PeriodicalId":18159,"journal":{"name":"Magnesium research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1684/mrh.2020.0461","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41674169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reactive oxygen species are implicated in altering magnesium homeostasis in endothelial cells exposed to high glucose.","authors":"Roberta Scrimieri,&nbsp;Laura Locatelli,&nbsp;Roberta Cazzola,&nbsp;Jeanette A M Maier,&nbsp;Alessandra Cazzaniga","doi":"10.1684/mrh.2019.0456","DOIUrl":"https://doi.org/10.1684/mrh.2019.0456","url":null,"abstract":"<p><p>Transient Receptor Potential Melastatin (TRPM)7 is important in maintaining the intracellular homeostasis of magnesium (Mg), which is instrumental for vital cellular functions. Since the upregulation of TRPM7 has been proposed as a marker of endothelial dysfunction, we evaluated the effects of high glucose, which markedly impacts endothelial performance, on TRPM7 and intracellular Mg homeostasis in human macrovascular endothelial cells. We show that glucose-induced free radicals increase the amounts of TRPM7 as well as total intracellular magnesium. On the contrary, the highly selective Mg transporter MagT1 is not modulated by high glucose, hydrogen peroxide and low extracellular magnesium. We conclude that in endothelial cells high glucose alters Mg homeostasis through the upregulation of TRPM7.</p>","PeriodicalId":18159,"journal":{"name":"Magnesium research","volume":"32 3","pages":"54-62"},"PeriodicalIF":3.2,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37729390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnesium transport and homeostasis-related gene expression in skeletal muscle of young and old adults: analysis of the transcriptomic data from the PROOF cohort Study.","authors":"Cécile Coudy-Gandilhon,&nbsp;Marine Gueugneau,&nbsp;Daniel Taillandier,&nbsp;Lydie Combaret,&nbsp;Cécile Polge,&nbsp;Frédéric Roche,&nbsp;Jean-Claude Barthélémy,&nbsp;Léonard Féasson,&nbsp;Jeanette A Maier,&nbsp;André Mazur,&nbsp;Daniel Béchet","doi":"10.1684/mrh.2019.0458","DOIUrl":"https://doi.org/10.1684/mrh.2019.0458","url":null,"abstract":"<p><p>Magnesium (Mg²⁺) is critical for a number of biological processes and 25% body Mg²⁺ is located in the skeletal muscle. Mg²⁺ transport and homeostasis systems (MgTHs) regulate intracellular Mg²⁺ concentration and muscle MgTHs are thus related to whole body Mg²⁺ homeostasis. Nonetheless, few studies have investigated the regulation of muscle MgTHs under (patho)physiological conditions. Herein, we assessed the relationship between the expression of MgTHs genes (Trpm6, Trpm7, Magt1, Mrs2, Cnnm1-4, Slc41a1-3) and relevant pathways in human sarcopenia, which is one of the most dramatic physiologic changes affecting the human body. Transcriptomic data were compared between young adult (YO, 22 y, n = 11) and old (EL, 73 y, n = 13) men from the PROOF cohort. MgTH mRNA levels did not change with aging, with the exception of a slight decrease for Slc41a3. Nevertheless, interindividual variations of mRNA levels revealed strong correlations between MgTHs in the YO group, while few were maintained in the EL muscle. Moreover, in the YO muscle, different clusters of MgTH mRNAs strongly correlated with divers physiological (BMI, blood pressure) and muscle characteristics (intramyocellular droplets, capillarization); however, most correlations changed or disappeared in the EL muscle. Further investigations of the whole transcriptome identified several sets of mRNAs correlated with defined MgTHs. There again was a sharp difference between YO and EL muscles, as the number of mRNAs correlated with MgTHs strongly decreased with aging. Gene ontology analyses of these sets of correlated mRNAs revealed 6 biological processes common to YO and EL, 3 specific to the YO (RNA processing, translation, respiration), and 2 (regulation of catabolic process, Wnt signaling) to the EL muscle. Overall, these observations lead to questions about potential resilience to muscle Mg²⁺ homeostasis in the elderly.</p>","PeriodicalId":18159,"journal":{"name":"Magnesium research","volume":"32 3","pages":"72-82"},"PeriodicalIF":3.2,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37729393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of bioavailability of Mg from Mg citrate and Mg oxide by measuring urinary excretion in Mg-saturated subjects.","authors":"Tanja Werner,&nbsp;Martin Kolisek,&nbsp;Jürgen Vormann,&nbsp;Ivana Pilchova,&nbsp;Marian Grendar,&nbsp;Eva Struharnanska,&nbsp;Michal Cibulka","doi":"10.1684/mrh.2019.0457","DOIUrl":"https://doi.org/10.1684/mrh.2019.0457","url":null,"abstract":"<p><strong>Background: </strong>Low magnesium (Mg) levels are linked to many diseases. Studies suggest that organic salts of Mg are more readily bioavailable than its oxide or inorganic salts used for supplements production. Unfortunately, the plethora of variables in the previous study designs complicates the making of any clear and reliable conclusions.</p><p><strong>Methods: </strong>14 healthy males were supplemented for five days with 400 mg Mg to saturate Mg pools before intake of the test products. Bioavailability of 400 mg Mg from Mg citrate (MgC) and Mg oxide (MgO) after single-dose administration was assessed by measuring renal Mg excretion in 24-h urine and blood plasma [Mg] at time points 0, 2, 4, 8, and 24 h.</p><p><strong>Results: </strong>Single-dose MgC supplementation led to a significant (P < 0.05) increase in 24 h urinary Mg excretion, but this was not significant following MgO. Plasma [Mg] was also significantly higher for MgC than for MgO at 4 h (P < 0.05) and 8 h (P < 0.05). Compared with baseline levels, MgC supplementation showed a significant increase in plasma [Mg] at all time points, in contrast to MgO.</p><p><strong>Conclusions: </strong>MgC shows higher bioavailability compared with MgO. Furthermore, urinary Mg excretion should be determined as the primary endpoint of Mg bioavailability studies.</p>","PeriodicalId":18159,"journal":{"name":"Magnesium research","volume":"32 3","pages":"63-71"},"PeriodicalIF":3.2,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37729392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum magnesium concentrations in vitiligo patients.","authors":"M R Namazi,&nbsp;Farnoosh Nozari,&nbsp;Peyman Jafari","doi":"10.1684/mrh.2019.0450","DOIUrl":"https://doi.org/10.1684/mrh.2019.0450","url":null,"abstract":"<p><p>Hypomagnesemia has been shown to contribute to oxidative stress and inflammation. This study was designed to evaluate the serum magnesium (Mg) concentration in vitiligo patients versus controls. Twenty-seven patients with vitiligo were enrolled in this study, along with 27 age and sex-matched healthy controls. The mean serum Mg concentrations in the case and control groups were 0.75 ± 0.07 and 0.77 ± 0.07 mmol/L, respectively. No significant difference in the mean concentrations of Mg between the two groups was noted (P = 0.95). However, interestingly, we noticed a positive correlation between serum Mg concentration and Vitiligo Area Severity Index (VASI) score as well as the total body surface area (TBSA) concerned by the disease. Further research on the role of Mg in vitiligo is therefore warranted.</p>","PeriodicalId":18159,"journal":{"name":"Magnesium research","volume":"32 2","pages":"51-53"},"PeriodicalIF":3.2,"publicationDate":"2019-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37489579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute myocardial infarction severity, complications, and mortality associated with lack of magnesium intake through consumption of desalinated seawater.","authors":"Meital Shlezinger, Y. Amitai, I. Goldenberg, S. Atar, M. Shechter","doi":"10.1684/mrh.2019.0449","DOIUrl":"https://doi.org/10.1684/mrh.2019.0449","url":null,"abstract":"Drinking water (DW) is an important dietary source of magnesium. Israel has recently increased desalinated seawater (DSW) production for DW, but negligible magnesium content in DSW may pose a risk of hypomagnesemia and consequential adverse cardiovascular effects. Consecutive acute myocardial infarction (AMI) patients (n = 380, age 35-75 years), hospitalized in 2015-2017 with ST-segment elevation myocardial infarction (STEMI), were divided into two groups based on their domicile region having a major supply of DSW (n = 250, 65%) or not (non-DSW; n = 130, 35%). We evaluated admission serum magnesium concentrations in patients, magnesium levels in tap water, 1-year all-cause mortality, and major adverse cardiovascular events (MACE), including all-cause mortality, nonfatal myocardial infarction, rehospitalization for heart failure or angina pectoris, stroke, coronary artery bypass grafting, and percutaneous coronary interventions. Multivariate analyses were adjusted for age and sex. Serum magnesium concentrations (mean ± SD) were significantly higher among patients in the non-DSW group compared with the DSW group (1.95 ± 0.20 mg/dL and 1.81 ± 0.20 mg/dL, P < 0.001; respectively). Additionally, the mean residential DW magnesium level in the DSW group was 5.4 ± 2.2 mg/L compared with 25.1 ± 3.4 mg/L, P < 0.01 in the non-DSW group. Fewer patients (although not statistically significant) in the non-DSW group experienced major adverse cardiovascular events (MACE) or 1-year-all-cause mortality compared with the patients in the DSW group (12.4% and 20%, P = 0.065; respectively). In conclusion, in post AMI patients, we found nonsignificant higher MACE and 1-year mortality with the use of DSW.","PeriodicalId":18159,"journal":{"name":"Magnesium research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46517866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correct estimates of hydrated ionic radius should be used when discussing the permeation of topically applied magnesium ions.","authors":"N. C. Chandrasekaran, R. Barnard","doi":"10.1684/mrh.2019.0451","DOIUrl":"https://doi.org/10.1684/mrh.2019.0451","url":null,"abstract":"","PeriodicalId":18159,"journal":{"name":"Magnesium research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2019-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48332590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}