Macromolecular bioscience最新文献_第6页

Orai1 is Required for Osteogenic Differentiation on Nanoparticulate Mineralized Collagen Glycosaminoglycan Materials. Orai1是纳米颗粒矿化胶原糖胺聚糖材料成骨分化所必需的。

IF 4.1 4区医学

Macromolecular bioscience Pub Date : 2025-07-13 DOI: 10.1002/mabi.202400636

Meiwand Bedar, Wei Chen, Xiaoyan Ren, Shahrzad Moghadam, Youngnam Kang, Kaavian Shariati, Kelly X Huang, Grace Rubino, Brendan A C Harley, Justine C Lee

{"title":"Orai1 is Required for Osteogenic Differentiation on Nanoparticulate Mineralized Collagen Glycosaminoglycan Materials.","authors":"Meiwand Bedar, Wei Chen, Xiaoyan Ren, Shahrzad Moghadam, Youngnam Kang, Kaavian Shariati, Kelly X Huang, Grace Rubino, Brendan A C Harley, Justine C Lee","doi":"10.1002/mabi.202400636","DOIUrl":"10.1002/mabi.202400636","url":null,"abstract":"The role of the extracellular matrix (ECM) in guiding cell fate has spurred the development of synthetic, ECM-inspired regenerative biomaterials. We previously described a porous, open-cell foam composed of nanoparticulate mineralized collagen glycosaminoglycan (MC-GAG), capable of inducing in vitro osteogenesis of primary human mesenchymal stem cells (hMSCs) and in vivo skull regeneration without adding growth factors or expanded progenitor cells. This study investigated the role of store-operated calcium entry (SOCE) in MC-GAG activity by evaluating the impact of Orai1, the pore-forming subunit of the calcium release-activated channel. Compared to nonmineralized collagen glycosaminoglycan (Col-GAG), hMSCs cultured on MC-GAG exhibited increased Orai1 expression. Chemical inhibition of SOCE using MRS1845 reduced the expression of both gene and protein of osteogenic markers, as well as mineralization on MC-GAG, while MRS1845 exerted no effects on Col-GAG materials. Orai1 knockdown similarly inhibited matrix mineralization and expression of the late osteogenic marker, bone sialoprotein-2 (BSP2), on MC-GAG, whereas no effects were notable on Col-GAG. Orai1 knockdown reduced ERK1/2 phosphorylation, suggesting that the osteogenic effects of Orai1 functioned downstream of ERK1/2 pathways. These findings suggest Orai1 is necessary for osteoprogenitor maturation and matrix mineralization in MC-GAG-mediated osteogenic differentiation.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00636"},"PeriodicalIF":4.1,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12371709/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Substrate-Dependent Variability in Viability and Angiogenic Marker Expression Among Three Endothelial Cell Subtypes: Insights for Artificial Tissue Vascularization. 三种内皮细胞亚型中生存能力和血管生成标志物表达的底物依赖性变异性：对人工组织血管化的见解。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-07-10 DOI: 10.1002/mabi.202500333

Jernej Vajda, Boštjan Vihar, Marko Milojević, Dragana Bjelić, Amadeja Brečko, Uroš Maver

{"title":"Substrate-Dependent Variability in Viability and Angiogenic Marker Expression Among Three Endothelial Cell Subtypes: Insights for Artificial Tissue Vascularization.","authors":"Jernej Vajda, Boštjan Vihar, Marko Milojević, Dragana Bjelić, Amadeja Brečko, Uroš Maver","doi":"10.1002/mabi.202500333","DOIUrl":"https://doi.org/10.1002/mabi.202500333","url":null,"abstract":"Tissue engineering faces the challenge of achieving effective vascularization within tissue constructs for sustained viability and optimal function. The success of tissue-engineered constructs depends on selecting an optimal angiogenesis-stimulating ECM substitute material. This study compares four substrates made from three different biomacromolecules-fibrin, fibronectin, non-crosslinked, and crosslinked gelatin, and their effect on endothelial cells. Acknowledging the diverse range of endothelial cells that play a role in (micro)vascularization, human endothelial primary cells, human umbilical vein endothelial cells, and human microvascular endothelial cells are subjected to these materials for evaluation. Biocompatibility is assessed by measuring cell viability (Live/Dead assay), metabolic activity (alamarBlue assay), morphology (actin staining), phenotype expression (immunocytochemistry), and the production of von Willebrand factor, which promotes angiogenesis by promoting cell adhesion and migration. The results show that the use of biomaterials as culturing substrates significantly impacts the viability and morphology of the cells. While the expression of angiogenic markers is shown to rely more on the cell lineage, the use of different substrates has an impact on the expression timeline. Thus, combining cells and biomaterials in a favorable manner can be used as a powerful tool for controlled vascularization in vitro, which requires the systematic assembly of different stimuli.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00333"},"PeriodicalIF":4.4,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144600928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Continuous Chitosan/Poly (Vinyl Alcohol) Nanofiber in Collagen Hydrogel to Prepare Mechanically Robust Fibrous Nanocomposite for Tissue Engineering 连续壳聚糖/聚乙烯醇纳米纤维在胶原水凝胶中制备组织工程用机械坚固的纳米纤维复合材料。

IF 4.1 4区医学

Macromolecular bioscience Pub Date : 2025-07-07 DOI: 10.1002/mabi.202500230

Shakiba Kalhori, Ayoob Karimizade, Mohsen Sadeghi-Ghadikolaei, Masoud Siaghi, Amir Mellati, Somayeh Shahani

{"title":"Continuous Chitosan/Poly (Vinyl Alcohol) Nanofiber in Collagen Hydrogel to Prepare Mechanically Robust Fibrous Nanocomposite for Tissue Engineering","authors":"Shakiba Kalhori, Ayoob Karimizade, Mohsen Sadeghi-Ghadikolaei, Masoud Siaghi, Amir Mellati, Somayeh Shahani","doi":"10.1002/mabi.202500230","DOIUrl":"10.1002/mabi.202500230","url":null,"abstract":"<div>\u0000 \u0000 Collagen (Col) hydrogel scaffolds require mechanical properties comparable to those of implanted tissues; however, their significant shrinkage, opacity, and rapid degradation hinder their application in tissue engineering and therapeutic contexts. Furthermore, polymer-reinforced Col hydrogels often lose their fibrous morphology, leading to a reduction in cell binding sites. In this study, we aim to enhance the mechanical properties and biodegradation resistance of Col hydrogels while preserving their fibrous microstructure. We achieve this by blending Col hydrogel with a continuous chitosan/poly(vinyl alcohol) nanofiber suspension (CS/PVA@NF), utilizing a wet electrospinning process coupled with a falling film collector. Morphological assessment of CS/PVA@NF reveals a well-defined nanofibrous microstructure, in contrast to the non-fibrous morphology observed in conventional polymer-blended hydrogels. The mechanical properties of the composite hydrogel improve up to 24-fold (Young's modulus: 120 kPa). The incorporation of CS/PVA@NF enhances cell attachment and proliferation potential. Subcutaneous implantation of the hydrogels in a murine model shows no notable inflammation. This research presents an effective method for improving Col hydrogels while maintaining their nanofibrous structure.\u0000 </div>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"25 9","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144584292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering Efferocytosis for Bone Regeneration. 骨再生的工程Efferocytosis。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-07-04 DOI: 10.1002/mabi.202500094

Jacob Miszuk, Linna Zhong, Hongli Sun

{"title":"Engineering Efferocytosis for Bone Regeneration.","authors":"Jacob Miszuk, Linna Zhong, Hongli Sun","doi":"10.1002/mabi.202500094","DOIUrl":"10.1002/mabi.202500094","url":null,"abstract":"Bone is an incredibly robust tissue thanks to its high blood supply, rapid cell turnover, and continuous remodeling. A significant body of research investigates strategies to improve osteogenesis, angiogenesis, and immunomodulation for bone regeneration, facilitated by numerous various therapeutic approaches (e.g. pharmacologics, biomaterials, stem cell therapy, and more). However, a critically understudied but recently emerging area of research lies in the inflammatory cascade and the cleanup of apoptotic cells during repair, aging, and disease. Termed \"efferocytosis,\" this natural and efficient cleaning up of cells at the end of their lifespan is a crucial step in resolving injury, controlling disease, maintaining homeostasis, and tissue repair. Currently, the primary mechanism(s) driving efferocytosis in most tissue but especially bone, is unknown. Despite this knowledge gap, mounting evidence suggests that impaired efferocytosis plays a significant role in many chronic illnesses and impairs tissue regeneration. Biomaterials-based interventions are well-positioned to interrogate mechanisms of efferocytosis due to their ability to provide local support and guide cellular activity not only in combination with but also without additional pharmaceutical aid. This review will highlight the current understanding of efferocytosis in bone and discuss cutting-edge biomaterials-based strategies to engineer efferocytosis for improved outcomes in bone regeneration.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00094"},"PeriodicalIF":4.4,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12261927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144564871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Injectable Multifunctional Hemostatic Adhesive for the Hemostasis of Non-Compressible Hemorrhage and Anti-Infection of Bacterial Wounds. 注射用多功能止血胶粘剂用于不可压缩性出血止血及细菌性创面抗感染。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-07-02 DOI: 10.1002/mabi.202500294

Yu-Yao Li, Xiao-Hong Zhu, Zedong Cai, Xiao-Suan Zhou, Wei Wang, Ya-Jun Shi, Bang-Le Zhang

{"title":"Injectable Multifunctional Hemostatic Adhesive for the Hemostasis of Non-Compressible Hemorrhage and Anti-Infection of Bacterial Wounds.","authors":"Yu-Yao Li, Xiao-Hong Zhu, Zedong Cai, Xiao-Suan Zhou, Wei Wang, Ya-Jun Shi, Bang-Le Zhang","doi":"10.1002/mabi.202500294","DOIUrl":"https://doi.org/10.1002/mabi.202500294","url":null,"abstract":"Non-compressible hemorrhage and bacterial infection remain as the primary challenges in emergency surgery and war trauma. To better control hemorrhage immediately from non-compressible wounds, an injectable double-network multifunctional hemostatic adhesive GelMA/QCS/Ca2+ based on gelatin methacryloyl (GelMA), quaternary ammonium chitosan (QCS) and calcium ions is designed. The adhesive formation is based on the blue light-triggered crosslinking of GelMA and the synergistic hydrogen bond between GelMA and QCS. The results show that the fast adhesive triggered by blue light provides rapid hemostatic ability through sealing blood vessels, including tail amputation, puncture femoral artery, liver puncture, and hepatectomy. A series of in vitro and in vivo hemostatic and antibacterial models in mice indicate that GelMA/QCS/Ca2+ adhesive exhibits better hemostatic and antibacterial abilities than the commercially available adhesive fibrin glue and the hemostatic hydrogels with a single function. GelMA/QCS/Ca2+ adhesive has broad application prospects as a hemostatic wound dressing in the emergency treatment of non-compressible hemorrhage and bacterial infection of wounds.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00294"},"PeriodicalIF":4.4,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144553932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Advances in Injectable Hydrogel Biotherapeutics for Regenerative Dental Medicine. 再生牙科注射用水凝胶生物疗法研究进展。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-07-02 DOI: 10.1002/mabi.202500096

Renan Dal-Fabbro, Arwa Daghrery, Caroline Anselmi, Igor Paulino M Soares, Alexandre Henrique Dos Reis-Prado, Pedro Henrique Chaves de Oliveira, Marco C Bottino

{"title":"Recent Advances in Injectable Hydrogel Biotherapeutics for Regenerative Dental Medicine.","authors":"Renan Dal-Fabbro, Arwa Daghrery, Caroline Anselmi, Igor Paulino M Soares, Alexandre Henrique Dos Reis-Prado, Pedro Henrique Chaves de Oliveira, Marco C Bottino","doi":"10.1002/mabi.202500096","DOIUrl":"https://doi.org/10.1002/mabi.202500096","url":null,"abstract":"Dental caries and periodontitis continue to be significant oral health challenges. Traditional therapies typically manage infection and inflammation but seldom restore lost tissue. In this way, injectable hydrogels have emerged as versatile biomaterials that adapt to the complex anatomy of the dentin-pulp complex and periodontal lesions. Generally speaking, by incorporating antimicrobial, anti-inflammatory, and pro-regenerative cues, these hydrogels support localized disinfection, promote angiogenesis, and foster tissue regeneration. More specifically, recent advances in functionalizing hydrogels with growth factors, bioactive compounds, and dentin matrix components have effectively modulated inflammatory responses, stimulated host cell activity, and facilitated the formation of hard and soft tissue for regenerative endodontic therapies. In periodontology, engineered hydrogels have demonstrated their ability to deliver osteogenic, antimicrobial, and immunomodulatory agents while also enabling the encapsulation of stem cells or extracellular vesicles, thereby enhancing cellular activity and supporting the regeneration of alveolar bone and soft tissue. This review summarizes recent advancements in injectable hydrogels for regenerating the dentin-pulp complex and periodontal tissues, discussing their design principles, performance, and future directions. Although not exhaustive, it provides a comprehensive overview to inspire further research and innovation, encouraging the development of novel, easy-to-apply, and highly biocompatible multifunctional hydrogels for regenerative dental medicine.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00096"},"PeriodicalIF":4.4,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144553933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silk Fibroin-Based Matrices for the Guidance of Cell Interaction, Tissue Regeneration, and Crosstalk. 基于丝素蛋白的基质用于细胞相互作用、组织再生和串扰的引导。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-06-29 DOI: 10.1002/mabi.202400629

Behnaz Sadat Eftekhari, Behnaz Ashtari, Mozhgan Jahani, Elham Afjeh-Dana, Paul A Janmey, Sara Simorgh, Mazaher Gholipourmalekabadi

{"title":"Silk Fibroin-Based Matrices for the Guidance of Cell Interaction, Tissue Regeneration, and Crosstalk.","authors":"Behnaz Sadat Eftekhari, Behnaz Ashtari, Mozhgan Jahani, Elham Afjeh-Dana, Paul A Janmey, Sara Simorgh, Mazaher Gholipourmalekabadi","doi":"10.1002/mabi.202400629","DOIUrl":"https://doi.org/10.1002/mabi.202400629","url":null,"abstract":"The interactions between cells and the extracellular matrix are essential regulators of cell behaviors such as adhesion, proliferation, migration, differentiation, and function. From the perspective of tissue regeneration, some physicochemical characteristics of the material, including hydrophilicity, topology, and charge of the material surface, can significantly affect cell adhesion, proliferation, and differentiation. Many biomaterials are introduced for tissue engineering scaffolds, biomimicking natural tissues. Among the biomaterials, silk proteins (fibroin and sericin) have many excellent characteristics, making them ideal candidates for regenerative medicine. Several studies have tuned silk fibroin characteristics to specify cell adhesion, proliferation, and stem cell differentiation by combining fibroin with other materials, coating, modification, and biofunctionalization. In the current review article, the essential properties of silk fibroin-based scaffolds (presence of cell adhesion motifs, wettability, charge, elasticity) and their influences on cell adhesion, proliferation, and migration, as well as their biodegradation and the body's immune response are discussed. In addition, the crosstalk between silk fibroin and various cells is discussed, as well as different methods for blending or biofunctionalization of silk fibroin with the aim of engineering a silk-based scaffold with a specifically tuned response to biological systems and subsequently affecting the behavior of the cells.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00629"},"PeriodicalIF":4.4,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Tissue Engineering's Guide to Biomimicry. 组织工程仿生指南。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-06-29 DOI: 10.1002/mabi.202500093

Kenny A van Kampen, Carlos Mota, Lorenzo Moroni

引用次数: 0

Fabrication of Lithium/Strontium-Releasing Smart Bioactive Glasses with Anti-Inflammatory and Osteogenic Effects Tailored to Pathological Stages. 具有抗炎和成骨作用的锂/锶释放智能生物活性眼镜的制备

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-06-29 DOI: 10.1002/mabi.202500268

Hirohiko Sakai, Jun-Ichi Sasaki, Haruaki Kitagawa, Gabriela L Abe, Tomoki Kohno, Naoya Funayama, Satoshi Imazato

{"title":"Fabrication of Lithium/Strontium-Releasing Smart Bioactive Glasses with Anti-Inflammatory and Osteogenic Effects Tailored to Pathological Stages.","authors":"Hirohiko Sakai, Jun-Ichi Sasaki, Haruaki Kitagawa, Gabriela L Abe, Tomoki Kohno, Naoya Funayama, Satoshi Imazato","doi":"10.1002/mabi.202500268","DOIUrl":"https://doi.org/10.1002/mabi.202500268","url":null,"abstract":"Bioactive glasses (BGs) are highly biocompatible with affinity for hard tissues and exhibit high bioactivity through ion release. Smart BGs that allow controlled ion release are required because uncontrolled release can lead to unexpected adverse effects on tissue regeneration. Strontium promotes osteoblast differentiation of mesenchymal stem cells (MSCs) and inhibits osteoclast activity. In this study, the release profile of strontium is regulated by the incorporation of aluminum into a phosphate-based BG. Furthermore, composites of strontium-releasing BG and lithium-releasing BG (Li/Sr-BG) show stepwise ion release, with rapid lithium release followed by sustained strontium release. Li/Sr-BG increases the expression of osteogenic markers and mineral deposition in MSCs, but suppresses osteoclast maturation, including multinucleation and osteoclast marker expression. Additionally, application of Li/Sr-BG to inflammatory macrophages decreases phagocytic activity and inflammatory gene expression, while increasing the expression of anti-inflammatory markers. Analysis of signaling proteins reveals that osteogenic and anti-inflammatory effects of Li/Sr-BG are attributed to the release of strontium and lithium, respectively. This study demonstrates that Li/Sr-BGs can be used for the development of novel smart bioactive materials that effectively suppress inflammation and promote bone formation in a manner that follows the process of bone regeneration.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00268"},"PeriodicalIF":4.4,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanoparticle-Embedded GelMA/NIPAm Hydrogels: A Temperature-Responsive Hybrid System for Controlled Drug Release. 纳米颗粒嵌入凝胶/NIPAm水凝胶：用于控制药物释放的温度响应混合系统。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-06-29 DOI: 10.1002/mabi.202400635

Maria Daaboul, Ayse Akkaya, Zehra Kanli, Oguzhan Gunduz, Banu Aydın, Emine Alarcin, Mehmet Murat Ozmen, Murat Topuzogullari

{"title":"Nanoparticle-Embedded GelMA/NIPAm Hydrogels: A Temperature-Responsive Hybrid System for Controlled Drug Release.","authors":"Maria Daaboul, Ayse Akkaya, Zehra Kanli, Oguzhan Gunduz, Banu Aydın, Emine Alarcin, Mehmet Murat Ozmen, Murat Topuzogullari","doi":"10.1002/mabi.202400635","DOIUrl":"https://doi.org/10.1002/mabi.202400635","url":null,"abstract":"Temperature-responsive hydrogels incorporating drug-loaded polymeric nanoparticles represent a significant advancement in controlled release systems, enabling responsive and environmentally triggered drug delivery. In this study, a novel temperature-responsive drug delivery system was developed based on a gelatin methacryloyl/N-isopropylacrylamide (GelMA/NIPAm) hydrogel incorporating phenytoin (PHT)-loaded poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles. For this, empty nanoparticles, PHT-loaded nanoparticles, bare hydrogels (BH), empty nanoparticle-loaded hydrogels (eNP-H), and PHT-encapsulated nanoparticle-embedded hydrogels (PHT-H) were prepared and characterized using FTIR, SEM, DSC, XRD, DLS, swelling, drug release, and biocompatibility tests. The drug-loaded nanoparticles exhibited hydrodynamic diameter of 223.7 ± 8.4 nm with a PDI of 0.298 and a zeta potential of -20.4 mV. The BH, eNP-H, and PHT-H hydrogels displayed similar temperature-dependent swelling, with approximate weight swelling ratios of 9.0 at 25°C, 7.5 at 37°C, and 6.0 at 40°C. Swelling kinetics showed that all hydrogels reached equilibrium within 20 min. Moreover, the hydrogels demonstrated consistent cyclic swelling and shrinking at 37°C and 40°C. Drug release studies revealed that PHT-H hydrogels released ∼20% of phenytoin at 37°C and ∼34% at 40°C over 7 days, confirming sustained, temperature-responsive drug release. Cell viability assays indicated no cytotoxicity and potential promotion of cell proliferation. Thus, these hydrogels offer a promising platform for efficient, temperature-sensitive, and controlled drug delivery applications.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00635"},"PeriodicalIF":4.4,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0