Macromolecular bioscience最新文献_第6页

Masthead: Macromol. Biosci. 8/2024 刊头：Macromol.Biosci.8/2024

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-08-14 DOI: 10.1002/mabi.202470019

引用次数: 0

Nanoclay Hydrogel Microspheres with a Sandwich-Like Structure for Complex Tissue Infection Treatment 用于治疗复杂组织感染的具有三明治结构的纳米粘土水凝胶微球

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-08-14 DOI: 10.1002/mabi.202470018

Kunyuan Han, Jishizhan Chen, Qinglin Han, Lei Sun, Xieping Dong, Gengqiang Shi, Runhuai Yang, Wenqing Wei, Yunzhang Cheng

引用次数: 0

Dual-Functional Implant Based on Gellan-Xanthan Hydrogel with Diopside, BMP-2 and Lysostaphin for Bone Defect Repair and Control of Staphylococcal Infection. 基于含有地奥赛德、BMP-2 和溶血素的结冷黄原水凝胶的双功能植入体，用于骨缺损修复和葡萄球菌感染控制。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-08-14 DOI: 10.1002/mabi.202400205

Anna S Karyagina, Alexander V Grishin, Alina G Kudinova, Inna N Bulygina, Elizaveta V Koudan, Polina A Orlova, Vera P Datsenko, Anna V Zhulina, Tatyana M Grunina, Maria S Poponova, Mikhail S Krivozubov, Maria S Gromova, Natalia V Strukova, Maria S Generalova, Kirill E Nikitin, Igor V Shchetinin, Lev O Luchnikov, Svetlana V Zaitseva, Maria A Kirsanova, Eugene S Statnik, Fedor S Senatov, Vladimir G Lunin, Alexander V Gromov

{"title":"Dual-Functional Implant Based on Gellan-Xanthan Hydrogel with Diopside, BMP-2 and Lysostaphin for Bone Defect Repair and Control of Staphylococcal Infection.","authors":"Anna S Karyagina, Alexander V Grishin, Alina G Kudinova, Inna N Bulygina, Elizaveta V Koudan, Polina A Orlova, Vera P Datsenko, Anna V Zhulina, Tatyana M Grunina, Maria S Poponova, Mikhail S Krivozubov, Maria S Gromova, Natalia V Strukova, Maria S Generalova, Kirill E Nikitin, Igor V Shchetinin, Lev O Luchnikov, Svetlana V Zaitseva, Maria A Kirsanova, Eugene S Statnik, Fedor S Senatov, Vladimir G Lunin, Alexander V Gromov","doi":"10.1002/mabi.202400205","DOIUrl":"https://doi.org/10.1002/mabi.202400205","url":null,"abstract":"A new dual-functional implant based on gellan-xanthan hydrogel with calcium-magnesium silicate ceramic diopside and recombinant lysostaphin and bone morphogenetic protein 2 (BMP-2)-ray is developed. In this composite, BMP-2 is immobilized on microparticles of diopside while lysostaphin is mixed directly into the hydrogel, providing sustained release of BMP-2 to allow gradual bone formation and rapid release of lysostaphin to eliminate infection immediately after implantation. Introduction of diopside of up to 3% (w/v) has a negligible effect on the mechanical properties of the hydrogel but provides a high sorption capacity for BMP-2. The hydrogels show good biocompatibility and antibacterial activity. Lysostaphin released from the implants over a 3 h period efficiently kills planktonic cells and completely destroys 24 h pre-formed biofilms of Staphylococcus aureus. Furthermore, in vivo experiments in a mouse model of critically-sized cranial defects infected with S. aureus show a complete lack of osteogenesis when implants contain only BMP-2, whereas, in the presence of lysostaphin, complete closure of the defect with newly formed mineralized bone tissue is observed. Thus, the new implantable gellan-xanthan hydrogel with diopside and recombinant lysostaphin and BMP-2 shows both osteogenic and antibacterial properties and represents a promising material for the treatment and/or prevention of osteomyelitis after bone trauma.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141976098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface Immobilization of Oxidized Carboxymethyl Cellulose on Polyurethane for Sustained Drug Delivery. 氧化羧甲基纤维素在聚氨酯上的表面固定化用于持续给药

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-08-12 DOI: 10.1002/mabi.202400229

Manali Somani, Chetna Verma, Flavius Phrangsngi Nonglang, Surya Bhan, Bhuvanesh Gupta

{"title":"Surface Immobilization of Oxidized Carboxymethyl Cellulose on Polyurethane for Sustained Drug Delivery.","authors":"Manali Somani, Chetna Verma, Flavius Phrangsngi Nonglang, Surya Bhan, Bhuvanesh Gupta","doi":"10.1002/mabi.202400229","DOIUrl":"https://doi.org/10.1002/mabi.202400229","url":null,"abstract":"Polyurethane (PU) has a diverse array of customized physical, chemical, mechanical, and structural characteristics, rendering it a superb option for biomedical applications. The current study involves modifying the polyurethane surface by the process of aminolysis (aminolyzed polyurethane; PU-A), followed by covalently immobilizing Carboxymethyl cellulose (CMC) polymer utilizing Schiff base chemistry. Oxidation of CMC periodically leads to the creation of dialdehyde groups along the CMC chain. When the aldehyde groups on the OCMC contact the amine group on a modified PU surface, they form an imine bond. Scanning electron microscopy (SEM), contact angle, and X-ray photoelectron spectroscopy (XPS) techniques are employed to analyze and confirm the immobilization of OCMC on aminolyzed PU film (PU-O). The OCMC gel incorporates Nitrofurantoin (NF) and immobilizes it on the PU surface (PU-ON), creating an antibacterial PU surface. The confirmation of medication incorporation is achieved using EDX analysis. The varying doses of NF have demonstrated concentration-dependent bacteriostatic and bactericidal effects on both Gram-positive and Gram-negative bacteria, in addition to sustained release. The proposed polyurethane (PU-ON) surface exhibited excellent infection resistance in in vivo testing. The material exhibited biocompatibility and is well-suited for biomedical applications.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141917031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Star-Like Polypeptides as Simplified Analogues of Horseradish Peroxidase (HRP) Metalloenzymes 作为辣根过氧化物酶 (HRP) 金属酶简化类似物的星状多肽。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-08-09 DOI: 10.1002/mabi.202400155

Antoine Tronnet, Pedro Salas-Ambrosio, Rosa Roman, Lourdes Monica Bravo-Anaya, Marcela Ayala, Colin Bonduelle

引用次数: 0

Enhanced Osteogenesis in 2D and 3D Culture Systems Using RGD Peptide and α-TCP Phase Transition within Alginate-Based Hydrogel 利用藻酸盐水凝胶中的 RGD 肽和α-TCP 相转变增强二维和三维培养系统中的骨生成。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-08-08 DOI: 10.1002/mabi.202400190

Jueun Kim, Yeong-Jin Choi, Chang-Woo Gal, Aram Sung, Siwi Setya Utami, Honghyun Park, Hui-suk Yun

{"title":"Enhanced Osteogenesis in 2D and 3D Culture Systems Using RGD Peptide and α-TCP Phase Transition within Alginate-Based Hydrogel","authors":"Jueun Kim, Yeong-Jin Choi, Chang-Woo Gal, Aram Sung, Siwi Setya Utami, Honghyun Park, Hui-suk Yun","doi":"10.1002/mabi.202400190","DOIUrl":"10.1002/mabi.202400190","url":null,"abstract":"Cell-laden hydrogels have been extensively investigated in various tissue engineering fields by their potential capacity to deposit numerous types of cells in a specific area. They are largely used in soft-tissue engineering applications because of their low mechanical strength. In addition, sodium alginate is well-known for its encapsulation, loading capacity and for being easily controllable; however, it lacks cell-binding ligands and hence the ability to adhere cells. In this study, it is aimed to enhance osteogenesis in cells encapsulated in alginate and improve its mechanical properties by introducing a synthetic peptide and calcium phosphate phase transition. To increase cell–hydrogel interactions and increasing cell viability, an RGD peptide is added to a photocrosslinkable methacrylate-modified alginate, and alpha-tricalcium phosphate (α-TCP) is added to the hydrogel to increase its mechanical strength via phase transition. Cell proliferation, growth, and differentiation are assessed in both 2D and 3D cell cultures. The addition of α-TCP significantly improved the mechanical properties of the hydrogel. Moreover, the RGD peptide and α-TCP showed a synergistic effect with significantly improved cell adhesion and osteogenesis in both 2D and 3D cell cultures. Therefore, the functional hydrogel developed in this study can potentially be used for bone tissue regeneration.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202400190","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrogels with Antioxidant Microparticles Systems Based on Hyaluronic Acid for Regenerative Wound Healing 基于透明质酸的抗氧化微颗粒水凝胶系统用于伤口再生愈合。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-08-05 DOI: 10.1002/mabi.202400153

Isabella Nacu, Alina Ghilan, Alina G. Rusu, Maria Bercea, Loredana E. Nita, Liliana Vereştiuc, Aurica P. Chiriac

引用次数: 0

Fabrication of GelMA – Agarose Based 3D Bioprinted Photocurable Hydrogel with In Vitro Cytocompatibility and Cells Mirroring Natural Keratocytes for Corneal Stromal Regeneration 制作具有体外细胞相容性和天然角膜细胞镜像的基于琼脂糖的三维生物打印光固化水凝胶，用于角膜基质再生。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-08-03 DOI: 10.1002/mabi.202400136

Renuka Vijayaraghavan, Sravanthi Loganathan, Ravi Babu Valapa

{"title":"Fabrication of GelMA – Agarose Based 3D Bioprinted Photocurable Hydrogel with In Vitro Cytocompatibility and Cells Mirroring Natural Keratocytes for Corneal Stromal Regeneration","authors":"Renuka Vijayaraghavan, Sravanthi Loganathan, Ravi Babu Valapa","doi":"10.1002/mabi.202400136","DOIUrl":"10.1002/mabi.202400136","url":null,"abstract":"The complex anatomy of the cornea and the subsequent keratocyte-fibroblast transition have always made corneal stromal regeneration difficult. Recently, 3D printing has received considerable attention in terms of fabrication of scaffolds with precise dimension and pattern. In the current work, 3D printable polymer hydrogels made of GelMA/agarose are formulated and its rheological properties are evaluated. Despite the variation in agarose content, both the hydrogels exhibited G′>G′′ modulus. A prototype for 3D stromal model is created using Solid Works software, mimicking the anatomy of an adult cornea. The fabrication of 3D-printed hydrogels is performed using pneumatic extrusion. The FTIR analysis speculated that the hydrogel is well crosslinked and established strong hydrogen bonding with each other, thus contributing to improved thermal and structural stability. The MTT analysis revealed a higher rate of cell proliferation on the hydrogels. The optical analysis carried out on the 14th day of incubation revealed that the hydrogels exhibit transparency matching with natural corneal stromal tissue. Specific protein marker expression confirmed the keratocyte phenotype and showed that the cells do not undergo terminal differentiation into stromal fibroblasts. The findings of this work point to the potential of GelMA/A hydrogels as a novel biomaterial for corneal stromal tissue engineering.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sulfated Alginate for Biomedical Applications. 硫酸化海藻酸盐的生物医学应用。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-07-30 DOI: 10.1002/mabi.202400237

Alexandra L Mutch, Jiankun Yang, Vito Ferro, Anitha A, Lisbeth Grøndahl

{"title":"Sulfated Alginate for Biomedical Applications.","authors":"Alexandra L Mutch, Jiankun Yang, Vito Ferro, Anitha A, Lisbeth Grøndahl","doi":"10.1002/mabi.202400237","DOIUrl":"https://doi.org/10.1002/mabi.202400237","url":null,"abstract":"Alginate (Alg) polymers have received much attention due to the mild conditions required for gel formation and their good bio-acceptability. However, due to limited interactions with cells, many drugs, and biomolecules, chemically modified alginates are of great interest. Sulfated alginate (S-Alg) is a promising heparin-mimetic that continues to be investigated both as a drug molecule and as a component of biomaterials. Herein, the S-Alg literature of the past five years (2017-2023) is reviewed. Several methods used to synthesize S-Alg are described, with a focus on new advances in characterization and stereoselectivity. Material fabrication is another focus and spans bulk materials, particles, scaffolds, coatings, and part of multicomponent biomaterials. The new application of S-Alg as an antitumor agent is highlighted together with studies evaluating safety and biodistribution. The high binding affinity of S-Alg for various drugs and heparin-binding proteins is exploited extensively in biomaterial design to tune the encapsulation and release of these agents and this aspect is covered in detail. Recommondations include publishing key material properties to allow reproducibility, careful selection of appropriate sulfation strategies, the use of cross-linking strategies other than ionic cross-linking for material fabrication, and more detailed toxicity and biodistribution studies to inform future work.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141792862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanically Stable and Biocompatible Polymer Brush Coated Dental Materials with Lubricious and Antifouling Properties. 具有润滑和防污特性的机械稳定和生物兼容的聚合物刷涂牙科材料。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-07-29 DOI: 10.1002/mabi.202400194

Rong Mu, Ling Yang, Xinyue Wang, Binrui Yang, Jia Li, Aijun Wang, Guorui Zhang, Chufeng Sun, Yang Wu, Bo Yu, Bin Li

{"title":"Mechanically Stable and Biocompatible Polymer Brush Coated Dental Materials with Lubricious and Antifouling Properties.","authors":"Rong Mu, Ling Yang, Xinyue Wang, Binrui Yang, Jia Li, Aijun Wang, Guorui Zhang, Chufeng Sun, Yang Wu, Bo Yu, Bin Li","doi":"10.1002/mabi.202400194","DOIUrl":"https://doi.org/10.1002/mabi.202400194","url":null,"abstract":"Surface modification plays a crucial role in enhancing the functionality of implanted interventional medical devices, offering added advantages to patients, particularly in terms of lubrication and prevention of undesired adsorption of biomolecules and microorganisms, such as proteins and bacteria, on the material surfaces. Utilizing polymer brushes for surface modification is currently a promising approach to maintaining the inherent properties of materials while introducing new functionalities to surfaces. Here, surface-initiated atom transfer radical polymerization (SI-ATRP) technology to effectively graft anionic, cationic, and neutral polymer brushes from a mixed silane initiating layer is employed. The presence of a polymer brush layer significantly enhances the lubrication performance of the substrates and ensures a consistently low coefficient of friction over thousands of friction cycles in aqueous environments. The antimicrobial efficacy of polymer brushes is evaluated against gram-positive Staphylococcus aureus (S. aureus) and gram-negative Escherichia coli (E. coli). It is observed that polym er brushes grafted to diverse substrate surfaces displays notable antibacterial properties, effectively inhibiting bacterial attachment. Furthermore, the polymer brush layer shows favorable biocompatibility and anti-inflammatory characteristics, which shows potential applications in dental materials, and other fields such as catheters and food packaging.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0