Macromolecular bioscience最新文献_第10页

Sulfated Glycosaminoglycans as Inhibitors for Chlamydia Infections: Molecular Weight and Sulfation Dependence 磺化糖胺聚糖作为衣原体感染的抑制剂：分子量和磺化依赖性。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-01-21 DOI: 10.1002/mabi.202400443

Sebastian Wintgens, Janita Müller, Felicitas Drees, Dominik Spona, Lorand Bonda, Laura Hartmann, Johannes H. Hegemann, Stephan Schmidt

{"title":"Sulfated Glycosaminoglycans as Inhibitors for Chlamydia Infections: Molecular Weight and Sulfation Dependence","authors":"Sebastian Wintgens, Janita Müller, Felicitas Drees, Dominik Spona, Lorand Bonda, Laura Hartmann, Johannes H. Hegemann, Stephan Schmidt","doi":"10.1002/mabi.202400443","DOIUrl":"10.1002/mabi.202400443","url":null,"abstract":"Glycosaminoglycans (GAGs) play a pivotal role in pathogen attachment and entry into host cells, where the interaction with GAGs is critical for a diverse range of bacteria and viruses. This study focuses on elucidating the specific interactions between sulfated GAGs and the adhesin OmcB (Outer membrane complex protein B) of Chlamydia species, examining how structural characteristics of GAGs, such as sulfation degree and molecular weight, influence their binding affinity and thereby affect bacterial infectivity. A surface-based binding assay is established to determine the binding constants of OmcB with various GAGs. It is shown that increased sulfation and higher molecular weight enhance GAG binding to OmcB. These findings are further validated using cell assays, which shows that the addition of sulfated GAGs reduces OmcB-cell binding and inhibits the attachment of C. pneumoniae elementary bodies (EBs), underscoring the pivotal role of specific GAGs in chlamydial infections. Notably, heparin exhibites a stronger inhibitory effect on OmcB compare to GAGs with similar sulfation degrees and molecular weights, suggesting that particular molecular architectures may optimize binding interactions.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"25 4","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202400443","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143007971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating How All-Trans Retinoic Acid Polycaprolactone (atRA-PCL) Microparticles Alter the Material Properties of 3D Printed Fibrin Constructs 研究全反式维甲酸聚己内酯（atRA-PCL）微粒如何改变3D打印纤维蛋白结构的材料特性。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-01-21 DOI: 10.1002/mabi.202400464

Maria V. Hangad, Alejandro Forigua, Kali Scheck, Stephanie M. Willerth, Katherine S. Elvira

{"title":"Investigating How All-Trans Retinoic Acid Polycaprolactone (atRA-PCL) Microparticles Alter the Material Properties of 3D Printed Fibrin Constructs","authors":"Maria V. Hangad, Alejandro Forigua, Kali Scheck, Stephanie M. Willerth, Katherine S. Elvira","doi":"10.1002/mabi.202400464","DOIUrl":"10.1002/mabi.202400464","url":null,"abstract":"The 3D printing of human tissue constructs requires carefully designed bioinks to support the growth and function of cells. Here it is shown that an additional parameter is how drug-releasing microparticles affect the material properties of the scaffold. A microfluidic platform is used to create all-trans retinoic acid (atRA) polycaprolactone (PCL) microparticles with a high encapsulation efficiency (85.9 ± 5.0%), and incorporate them into fibrin constructs to investigate their effect on the material properties. An encapsulation that is around 25–35% higher than the current state of the art batch methods is achieved. It is also found that the drug loading concentration affects the microparticle size, which can be controlled using the microfluidic platform. It is shown that the release of atRA is slower in fibrin constructs than in buffer, and that the presence of atRA in the microparticles modulates both the degradation and the rheological properties of the constructs. Finally, it is shown that the fibrin material exhibits a stronger solid-like state in the presence of atRA-PCL microparticles. These findings establish a basis for understanding the interplay between drug-releasing microparticles and scaffold materials, paving the way for bioinks that achieve tailored degradation and mechanical properties, together with sustained drug delivery for tissue engineering applications.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"25 5","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202400464","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143007920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GelMA Hydrogels Integrated With aptamer CH6-Functionalized Tetrahedral DNA Nanostructures for Osteoporotic Mandibular Regeneration 与适配体ch6功能化四面体DNA纳米结构集成的凝胶用于骨质疏松颌骨再生。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-01-21 DOI: 10.1002/mabi.202400471

Shebin Hong, Ya Cui, Dongming He, Hao Wu, Weidong Jiang, Jian Cao, Xudong Wang

{"title":"GelMA Hydrogels Integrated With aptamer CH6-Functionalized Tetrahedral DNA Nanostructures for Osteoporotic Mandibular Regeneration","authors":"Shebin Hong, Ya Cui, Dongming He, Hao Wu, Weidong Jiang, Jian Cao, Xudong Wang","doi":"10.1002/mabi.202400471","DOIUrl":"10.1002/mabi.202400471","url":null,"abstract":"Osteoporotic bone regeneration is challenging due to impaired bone formation. Tetrahedral DNA nanostructures (TDN), promising nucleic acid nanomaterials, have garnered attention for their potential in osteoporotic mandibular regeneration owing to their ability to enhance cellular activity and promote osteogenic differentiation. Osteoblasts play a critical role in bone regeneration; however, intracellular delivery of TDN into osteoblasts remains difficult. In this study, a novel osteoblast-targeted CH6 aptamer-functionalized TDN (TDN-CH6) is aimed to develop for osteoporotic mandibular regeneration. This results demonstrated that TDN-CH6 exhibits superior osteoblast specificity and efficient recruitment to bone fracture sites. Furthermore, TDN-CH6 significantly enhances cellular activity and osteogenic differentiation compared to TDN alone. Notably, Gelatin Methacryloyl (GelMA) hydrogels incorporating TDN and TDN-CH6 shows improved biological performance and are favorable for osteoporotic mandibular regeneration, suggesting that this platform represents a promising strategy for addressing complex bone defects.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"25 4","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143007854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CP/HA/HGF Conductive Composite Scaffolds with Synergistic Electrical Stimulation for Nerve Regeneration CP/HA/HGF导电复合支架协同电刺激神经再生。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-01-21 DOI: 10.1002/mabi.202400265

Yahao Ma, Cong Wang, Jun Li, Pengfei Xie, Longyou Xiao, Seeram Ramakrishna, Nuan Chen, Xiaoying Wang, Liumin He

引用次数: 0

Designing Short Cardin-Motif Peptide and Biopolymer-Based Multicomponent Hydrogels for Developing Advanced Composite Scaffolds for Improving Cellular Behavior 设计短基序肽和基于生物聚合物的多组分水凝胶用于开发先进的复合支架以改善细胞行为。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-01-21 DOI: 10.1002/mabi.202400555

Sourav Sen, Rakesh Kumar, Rahul Singh Tomar, Sangita Roy

{"title":"Designing Short Cardin-Motif Peptide and Biopolymer-Based Multicomponent Hydrogels for Developing Advanced Composite Scaffolds for Improving Cellular Behavior","authors":"Sourav Sen, Rakesh Kumar, Rahul Singh Tomar, Sangita Roy","doi":"10.1002/mabi.202400555","DOIUrl":"10.1002/mabi.202400555","url":null,"abstract":"Multicomponent self-assembly represents a cutting-edge strategy in peptide nanotechnology, enabling the creation of nanomaterials with enhanced physical and biological characteristics. This approach draws inspiration from the highly complex nature of the native extracellular matrix (ECM) constituting multicomponent biomolecular entities. In recent years, the combination of bioactive peptide with polymer has gained significant attention for the fabrication of novel biomaterials due to their inherent specificity, tunable physiochemical properties, biocompatibility, and biodegradability. This advanced strategy can address the limitation of the lower mechanical strength of the individual peptide hydrogel by incorporating the biopolymer, resulting in the formation of a composite scaffold. In this direction, this advanced strategy is explored using noncovalent interactions between cellulose nano-fiber (CNF) and cationic Cardin-motif peptide to develop advanced composite scaffolds. The bioactive cationic peptide otherwise failed to form hydrogel at physiological conditions. Interestingly, the differential mixing ratio of CNF and peptide modulated the surface charge, functionality, and mechanical properties of the composite scaffolds, resulting in diverse cellular responses. 10:1 (w/w) ratio of CNF and peptide-based composite scaffold demonstrates improved cellular survival and proliferation in 2D culture conditions. Notably, in 3D cultures, cell proliferation on the 10:1 matrix is comparable to Matrigel, highlighting its potential for advanced tissue engineering applications.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"25 5","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143007849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing Hemocompatibility in ECMO Systems With a Fibrinolytic Interactive Coating: in Vitro Evaluation of Blood Clot Lysis Using a 3D Microfluidic Model 增强血液相容性在ECMO系统与纤溶相互作用涂层：在体外评估血凝块溶解使用三维微流体模型。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-01-13 DOI: 10.1002/mabi.202400530

Lena Witzdam, Samarth Sandhu, Suji Shin, Yeahwa Hong, Shanzeh Kamal, Oliver Grottke, Keith E. Cook, Cesar Rodriguez-Emmenegger

{"title":"Enhancing Hemocompatibility in ECMO Systems With a Fibrinolytic Interactive Coating: in Vitro Evaluation of Blood Clot Lysis Using a 3D Microfluidic Model","authors":"Lena Witzdam, Samarth Sandhu, Suji Shin, Yeahwa Hong, Shanzeh Kamal, Oliver Grottke, Keith E. Cook, Cesar Rodriguez-Emmenegger","doi":"10.1002/mabi.202400530","DOIUrl":"10.1002/mabi.202400530","url":null,"abstract":"Blood-contacting medical devices, especially extracorporeal membrane oxygenators (ECMOs), are highly susceptible to surface-induced coagulation because of their extensive surface area. This can compromise device functionality and lead to life-threatening complications. High doses of anticoagulants, combined with anti-thrombogenic surface coatings, are typically employed to mitigate this risk, but such treatment can lead to hemorrhagic complications. Therefore, bioactive surface coatings that mimic endothelial blood regulation are needed. However, evaluating these coatings under realistic ECMO conditions is both expensive and challenging. This study utilizes microchannel devices to simulate ECMO fluid dynamics and assess the clot-lysis efficacy of a self-activating fibrinolytic coating system. The system uses antifouling polymer brushes combined with tissue plasminogen activator (tPA) to induce fibrinolysis at the surface. Here, tPA catalyzes the conversion of blood plasminogen into plasmin, which dissolves clots. This positive feedback loop enhances clot digestion under ECMO-like conditions. This findings demonstrate that this coating system can significantly improve the hemocompatibility of medical device surfaces.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"25 5","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202400530","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

4-O-Methylglucuronoxylan from Hygrophila Ringens var. Ringens Seeds: Chemical Composition and Anti-Inflammatory Activity 4- o -甲基葡萄糖醛酸氧化酶来自喜湿草的种子：化学成分和抗炎活性。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-01-13 DOI: 10.1002/mabi.202400434

Vo Hoai Bac, Tat Cuong Trinh, Andreas Koschella, Thomas Heinze, Yu Ping Fu, Kari Tvete Inngjerdingen, Le Van Truong, Berit Smestad Paulsen, Martin Gericke

{"title":"4-O-Methylglucuronoxylan from Hygrophila Ringens var. Ringens Seeds: Chemical Composition and Anti-Inflammatory Activity","authors":"Vo Hoai Bac, Tat Cuong Trinh, Andreas Koschella, Thomas Heinze, Yu Ping Fu, Kari Tvete Inngjerdingen, Le Van Truong, Berit Smestad Paulsen, Martin Gericke","doi":"10.1002/mabi.202400434","DOIUrl":"10.1002/mabi.202400434","url":null,"abstract":"Hygrophila ringens var. ringens is a medicinal plant of the Acanthaceae family. A soluble polysaccharide is extracted from H. ringens seeds using warm water, followed by deproteinization and purification using column chromatography. DL1 is characterized comprehensively using spectroscopic and chromatographic techniques and identified as a polymer containing xylose (Xyl; 78.5%) and 4-O-methyl-d-glucuronic acid (4-O-MeGlcA; 21.5 %). The most prominent glycosidic linkages detected are terminal-xylose (T-Xyl); 1,2,3,4-Xylp; 1,2,4-Xylp; and T-4-O-MeGlcA. DL1 belongs to the xylan group and is a 4-O-methylglucuronoxylan. DL1 exhibits inhibition of bovine serum albumin denaturation with IC50 values of 0.35 mg mL−1 and a similar activity to diclofenac (non-steroidal anti-inflammatory drug). In a model of lipopolysaccharide-stimulated macrophages, DL1 (20–40 µg mL−1) strongly inhibits inflammatory cytokines and reactive oxygen species release without having significant macrophage cytotoxicity. The inhibitory effect of DL1 on inflammatory cytokines is mediated by the activation of mitogen-activated protein kinases by inhibiting the phosphorylation of p38 and extracellular signal-regulated kinase. These results highlight the potential of DL1 for treating inflammation through its cytokine-suppressive activity.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"25 4","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202400434","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Thin Polymer Layer Prepared from Liposomes and Polyelectrolytes for TGF-β3 Release in Tissue Engineering 脂质体和聚电解质制备TGF-β3在组织工程中释放的薄聚合物层的表征

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-01-13 DOI: 10.1002/mabi.202400447

Nils Meier, Leonie Berten-Schunk, Yvonne Roger, Robert Hänsch, Andrea Hoffmann, Heike Bunjes, Wibke Dempwolf, Henning Menzel

{"title":"Characterization of Thin Polymer Layer Prepared from Liposomes and Polyelectrolytes for TGF-β3 Release in Tissue Engineering","authors":"Nils Meier, Leonie Berten-Schunk, Yvonne Roger, Robert Hänsch, Andrea Hoffmann, Heike Bunjes, Wibke Dempwolf, Henning Menzel","doi":"10.1002/mabi.202400447","DOIUrl":"10.1002/mabi.202400447","url":null,"abstract":"Implant-integrated drug delivery systems that enable the release of biologically active factors can be part of an in situ tissue engineering approach to restore biological function. Implants can be functionalized with drug-loaded nanoparticles through a layer-by-layer assembly. Such coatings can release biologically active levels of growth factors. Sustained release is desired for many in vivo applications. The layer-by-layer technique also allows for the addition of extra layers, which can serve as “barriers” to delay the release. Electrospun Polycaprolactone (PCL) fiber mats are modified with a Chitosan (CS) grafted with PCL sidechains (CS-g-PCL24) and coated with transforming growth factor beta 3 (TGF-β3) loaded Chitosan/tripolyphosphate nanoparticles as a drug delivery system. Additional layers including polystyrene sulfonate, alginate, carboxymethyl cellulose, and liposomes (phosphatidylcholine) are applied. Streaming potential and X-ray photoelectron spectroscopy (XPS) measurements indicated a strong interpenetration of the chitosan and polyanion layers, while liposomes formed separate layers, which are more promising for sustained release. All samples release TGF-β3 at different cumulative levels without altering release kinetics. Variations in layer structure, interpenetration, and stability depending on the chitosan used are observed, which ultimately has minimal impact on the release kinetics. Polyelectrolyte layers strongly interpenetrated the active layers and therefore do not act as effective diffusion barriers, while the liposome layer, though separated, lacked sufficient stability.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"25 4","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202400447","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information: Macromol. Biosci. 1/2025 资料:宏mol。Biosci 1/2025。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-01-13 DOI: 10.1002/mabi.202570002

引用次数: 0

Effect of Defined Block Sequence Terpolymers on Antifungal Activity and Biocompatibility 限定嵌段序列三元共聚物对抗真菌活性和生物相容性的影响

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-01-07 DOI: 10.1002/mabi.202400429

Hatu Gmedhin, Sebastian Schaefer, Nathaniel Corrigan, Peifeng Wu, Zi Gu, Megan D. Lenardon, Cyrille Boyer

{"title":"Effect of Defined Block Sequence Terpolymers on Antifungal Activity and Biocompatibility","authors":"Hatu Gmedhin, Sebastian Schaefer, Nathaniel Corrigan, Peifeng Wu, Zi Gu, Megan D. Lenardon, Cyrille Boyer","doi":"10.1002/mabi.202400429","DOIUrl":"https://doi.org/10.1002/mabi.202400429","url":null,"abstract":"Invasive fungal infections cause over 3.7 million deaths worldwide annually, underscoring the critical need for new antifungal agents. Developing selective antifungal agents is challenging due to the shared eukaryotic nature of both fungal and mammalian cells. Toward addressing this, synthetic polymers designed to mimic host defense peptides are promising new candidates for combating fungal infections. This study investigates well-defined multiblock terpolymers with specific arrangements of cationic, hydrophobic, and hydrophilic groups, as potential antifungal agents. The block sequence in these copolymers significantly impacts their minimum inhibition concentration (MIC) against Candida albicans and biocompatibility. Furthermore, compared to their statistical counterparts, these block polymers exhibit lower MIC values in certain instances. Notably, triblock terpolymers containing a central hydrophobic block present an enhanced antifungal efficacy and biocompatibility. These findings highlight the potential of block sequence-controlled polymers as a versatile platform for developing customized and targeted antifungal therapies.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"25 4","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143826657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0