Macromolecular bioscience最新文献_第10页

Exploring Morphological and Molecular Properties of Different Adipose Cell Models: Monolayer, Spheroids, Gellan Gum-Based Hydrogels, and Explants 探索不同脂肪细胞模型的形态和分子特性：单层、球形、结冷胶水凝胶和外植体

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-10-25 DOI: 10.1002/mabi.202400320

Franziska B. Albrecht, Ann-Kathrin Schick, Annemarie Klatt, Freia F. Schmidt, Svenja Nellinger, Petra J. Kluger

{"title":"Exploring Morphological and Molecular Properties of Different Adipose Cell Models: Monolayer, Spheroids, Gellan Gum-Based Hydrogels, and Explants","authors":"Franziska B. Albrecht, Ann-Kathrin Schick, Annemarie Klatt, Freia F. Schmidt, Svenja Nellinger, Petra J. Kluger","doi":"10.1002/mabi.202400320","DOIUrl":"10.1002/mabi.202400320","url":null,"abstract":"White adipose tissue (WAT) plays a crucial role in energy homeostasis and secretes numerous adipokines with far-reaching effects. WAT is linked to diseases such as diabetes, cardiovascular disease, and cancer. There is a high demand for suitable in vitro models to study diseases and tissue metabolism. Most of these models are covered by 2D-monolayer cultures. This study aims to evaluate the performance of different WAT models to better derive potential applications. The stability of adipocyte characteristics in spheroids and two 3D gellan gum hydrogels with ex situ lobules and 2D-monolayer culture is analyzed. First, the differentiation to achieve adipocyte-like characteristics is determined. Second, to evaluate the maintenance of differentiated ASC-based models, an adipocyte-based model, and explants over 3 weeks, viability, intracellular lipid content, perilipin A expression, adipokine, and gene expression are analyzed. Several advantages are supported using each of the models. Including, but not limited to, the strong differentiation in 2D-monolayers, the self-assembling within spheroids, the long-term stability of the stem cell-containing hydrogels, and the mature phenotype within adipocyte-containing hydrogels and the lobules. This study highlights the advantages of 3D models due to their more in vivo-like behavior and provides an overview of the different adipose cell models.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"25 3","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202400320","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142503106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Viscosity Characteristics of Ideal Lubricants to Reduce Stress on Intestinal Mucosa During Balloon-Assisted Enteroscopy 理想润滑剂的粘度特性，以减轻球囊辅助肠镜检查过程中对肠道粘膜的压力。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-10-22 DOI: 10.1002/mabi.202400285

Katsuma Yamauchi, Ryohei Hirose, Hiroshi Ikegaya, Hiroki Mukai, Hajime Miyazaki, Naoto Watanabe, Takuma Yoshida, Risa Bandou, Ken Inoue, Osamu Dohi, Naohisa Yoshida, Takaaki Nakaya, Yoshito Itoh

{"title":"Viscosity Characteristics of Ideal Lubricants to Reduce Stress on Intestinal Mucosa During Balloon-Assisted Enteroscopy","authors":"Katsuma Yamauchi, Ryohei Hirose, Hiroshi Ikegaya, Hiroki Mukai, Hajime Miyazaki, Naoto Watanabe, Takuma Yoshida, Risa Bandou, Ken Inoue, Osamu Dohi, Naohisa Yoshida, Takaaki Nakaya, Yoshito Itoh","doi":"10.1002/mabi.202400285","DOIUrl":"10.1002/mabi.202400285","url":null,"abstract":"Balloon-assisted enteroscopy (BAE) is highly invasive and carries a higher risk of complications such as pain and perforation during enteroscope insertion. Applying lubricants to the small intestinal mucosa and reducing the dynamic friction coefficient (DFC) between the small intestinal mucosa and endoscopic shaft (ES) (or overtube (OT)) can minimize the invasiveness of BAE. However, the ideal viscosity characteristics of these lubricants remain unknown. In this study, a model is developed to measure the DFC using human small intestines from forensic autopsies and determine the ideal viscosity of low-friction lubricants that exhibit a minimal DFC, thus reducing the pressure on the intestinal lining during the procedure. The results reveal that the DFC is strongly correlated to the lubricant's viscosity rather than its chemical composition. Low-friction lubricants with viscosities within 0.20–0.32 and 0.35–0.58 Pa·s (at shear rates of 10 s−1) for the OT and ES, respectively, can significantly reduce the DFC, yielding optimal results. These findings highlight the role of viscosity in minimizing the friction between the equipment and small intestinal mucosa. The ideal low-friction lubricants satisfying the aforementioned viscosity ranges can minimize the invasiveness of BAE by decreasing the friction between the equipment and intestinal lining.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"24 12","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142503169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Facile Strategy for Preparing Flexible and Porous Hydrogel-Based Scaffolds from Silk Sericin/Wool Keratin by In Situ Bubble-Forming for Muscle Tissue Engineering Applications 利用原位气泡成形法制备柔性多孔水凝胶支架的简便策略，用于肌肉组织工程应用

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-10-20 DOI: 10.1002/mabi.202400362

Elif Beyza Demiray, Tugba Sezgin Arslan, Burak Derkus, Yavuz Emre Arslan

{"title":"A Facile Strategy for Preparing Flexible and Porous Hydrogel-Based Scaffolds from Silk Sericin/Wool Keratin by In Situ Bubble-Forming for Muscle Tissue Engineering Applications","authors":"Elif Beyza Demiray, Tugba Sezgin Arslan, Burak Derkus, Yavuz Emre Arslan","doi":"10.1002/mabi.202400362","DOIUrl":"10.1002/mabi.202400362","url":null,"abstract":"In the present study, it is aimed to fabricate a novel silk sericin (SS)/wool keratin (WK) hydrogel-based scaffolds using an in situ bubble-forming strategy containing an N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) coupling reaction. During the rapid gelation process, CO2 bubbles are released by activating the carboxyl groups in sericin with EDC and NHS, entrapped within the gel, creating a porous cross-linked structure. With this approach, five different hydrogels (S2K1, S4K2, S2K4, S6K3, and S3K6) are constructed to investigate the impact of varying sericin and keratin ratios. Analyses reveal that more sericin in the proteinaceous mixture reinforced the hydrogel network. Additionally, the hydrogels’ pore size distribution, swelling ratio, wettability, and in vitro biodegradation rate, which are crucial for the applications of biomaterials, are evaluated. Moreover, biocompatibility and proangiogenic properties are analyzed using an in-ovo chorioallantoic membrane assay. The findings suggest that the S4K2 hydrogel exhibited the most promising characteristics, featuring an adequately flexible and highly porous structure. The results obtained by in vitro assessments demonstrate the potential of S4K2 hydrogel in muscle tissue engineering. However, further work is necessary to improve hydrogels with an aligned structure to meet the features that can fully replace muscle tissue for volumetric muscle loss regeneration.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"25 2","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202400362","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142469036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silk Composite-Based Multifunctional Pellets for Controlled Release 基于蚕丝复合材料的多功能控释颗粒。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-10-20 DOI: 10.1002/mabi.202400410

Sushma Indrakumar, Sriram Bharath Gugulothu, Akshat Joshi, Tapan Kumar Dash, Vivek Mishra, Bharat Tandon, Kaushik Chatterjee

{"title":"Silk Composite-Based Multifunctional Pellets for Controlled Release","authors":"Sushma Indrakumar, Sriram Bharath Gugulothu, Akshat Joshi, Tapan Kumar Dash, Vivek Mishra, Bharat Tandon, Kaushik Chatterjee","doi":"10.1002/mabi.202400410","DOIUrl":"10.1002/mabi.202400410","url":null,"abstract":"Chronic wounds present significant clinical challenges due to the high risk of infections and persistent inflammation. While personalized treatments in point-of-care settings are crucial, they are limited by the complex fabrication techniques of the existing products. The calcium sulfate hemihydrate (CSH)-based drug delivery platform enables rapid fabrication but lacks antioxidant and antibacterial properties, essential to promote healing. To develop a multifunctional platform, a tannic acid (TA)-silk fibroin (SF) complex is engineered and incorporated as an additive in CSH cement. This cement is then cast into pellets to create silk/bioceramic-based composite drug delivery systems, designed for point-of-care use. Compared to neat CSH pellets, the composite pellets exhibit a 7.5-fold increase in antioxidant activity and prolonged antibacterial efficacy (up to 13 d). Moreover, the subcutaneous implantation of the pellets shows no hallmarks of local or systemic toxicity in a rodent model. The pellets are optimized in composition and fabrication to ease market translation. Clinically, the pellets have the potential to be further developed into products to place on wound beds or fill into bone cavities that are designed to deliver the intended therapeutic effect. The developed multifunctional system proves to be a promising solution for personalized treatment in point-of-care settings.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"25 2","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142469044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tunable Blended Collagen I/II and Collagen I/III Hydrogels as Tissue Mimics 作为组织模拟物的可调混合胶原 I/II 和胶原 I/III 水凝胶

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-10-20 DOI: 10.1002/mabi.202400280

Paulina M. Babiak, Carly M. Battistoni, Leonard Cahya, Rithika Athreya, Jason Minnich II, Alyssa Panitch, Julie C. Liu

{"title":"Tunable Blended Collagen I/II and Collagen I/III Hydrogels as Tissue Mimics","authors":"Paulina M. Babiak, Carly M. Battistoni, Leonard Cahya, Rithika Athreya, Jason Minnich II, Alyssa Panitch, Julie C. Liu","doi":"10.1002/mabi.202400280","DOIUrl":"10.1002/mabi.202400280","url":null,"abstract":"Collagen (Col) is commonly used as a natural biomaterial for biomedical applications. Although Col I is the most prevalent col type employed, many collagen types work together in vivo to confer function and biological activity. Thus, blending collagen types can better recapitulate many native environments. This work investigates how hydrogel properties can be tuned through blending collagen types (col I/II and col I/III) and by varying polymerization temperatures. Col I/II results in poorly developed fibril networks, which softened the gels, especially at lower polymerization temperatures. Conversely, col I/III hydrogels exhibit well-connected fibril networks with localized areas of fine fibrils and result in stiffer hydrogels. A decreased molecular mass recovery rate is observed in blended hydrogels. The altered fibril morphologies, mechanical properties, and biological signals of the blended gels can be leveraged to alter cell responses and can be used as models for different tissue types (e.g., healthy vs fibrotic tissue). Furthermore, the biomimetic hydrogel properties are a tool that can be used to modulate the transport of drugs, nutrients, and wastes in tissue engineering applications.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"24 12","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202400280","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142469045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecules in Motion: Unravelling the Dynamics of Vascularization Control in Tissue Engineering. 运动中的分子：揭示组织工程中血管控制的动力学。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-10-18 DOI: 10.1002/mabi.202400139

Francisco A. P. Rodrigues, Cláudia S. Oliveira, Simone C. Sá, Freni K. Tavaria, Sang Jin Lee, Ana L. Oliveira, João B. Costa

{"title":"Molecules in Motion: Unravelling the Dynamics of Vascularization Control in Tissue Engineering.","authors":"Francisco A. P. Rodrigues, Cláudia S. Oliveira, Simone C. Sá, Freni K. Tavaria, Sang Jin Lee, Ana L. Oliveira, João B. Costa","doi":"10.1002/mabi.202400139","DOIUrl":"10.1002/mabi.202400139","url":null,"abstract":"Significant progress has been made in tissue engineering (TE), aiming at providing personalized solutions and overcoming the current limitations of traditional tissue and organ transplantation. 3D bioprinting has emerged as a transformative technology in the field, able to mimic key properties of the natural architecture of the native tissues. However, most successes in the area are still limited to avascular or thin tissues due to the difficulties in controlling the vascularization of the engineered tissues. To address this issue, several molecules, biomaterials, and cells with pro- and anti-angiogenic potential have been intensively investigated. Furthermore, different bioreactors capable to provide a dynamic environment for in vitro vascularization control have been also explored. The present review summarizes the main molecules and TE strategies used to promote and inhibit vascularization in TE, as well as the techniques used to deliver them. Additionally, it also discusses the current challenges in 3D bioprinting and in tissue maturation to control in vitro/in vivo vascularization. Currently, this field of investigation is of utmost importance and may open doors for the design and development of more precise and controlled vascularization strategies in TE.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"24 12","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142469041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioengineered Silk Fibroin Hydrogel Reinforced with Collagen-Like Protein Chimeras for Improved Wound Healing 用胶原蛋白类嵌合体增强的生物工程蚕丝纤维素水凝胶可改善伤口愈合。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-10-18 DOI: 10.1002/mabi.202400346

Thiagarajan Hemalatha, Mayilvahanan Aarthy, Ashokraj Sundarapandiyan, Niraikulam Ayyadurai

{"title":"Bioengineered Silk Fibroin Hydrogel Reinforced with Collagen-Like Protein Chimeras for Improved Wound Healing","authors":"Thiagarajan Hemalatha, Mayilvahanan Aarthy, Ashokraj Sundarapandiyan, Niraikulam Ayyadurai","doi":"10.1002/mabi.202400346","DOIUrl":"10.1002/mabi.202400346","url":null,"abstract":"The study investigates the potentials of the rapid crosslinking hydrogel concoction comprising of natural silk fibroin (SF) and recombinant tailorable collagen-like protein with binding domains for wound repair. The formation of dityrosine crosslinks between the tyrosine moieties augments the formation of stable hydrogels, in the presence of the cytocompatible photo-initiator riboflavin and visible light. This uniquely engineered PASCH (Photo-activated silk fibroin and tailor-made collagen-like protein hydrogel) confers the key advantage of improved biological properties over the control hydrogels comprising only of SF. The physico-chemical characterization of the hydrogels with respect to crosslinking, modulus, and thermal stability delineates the ascendancy of PASCH 7:3 over other combinations. Furthermore, the hybrid protein hydrogel proves to be a favorable cellular matrix as it enhances cell adhesion, elongation, growth, and proliferation in vitro. Time-lapse microscopy studies reveal an enhanced wound closure in human endothelial cell monolayer (EA.hy926), while the gene expression studies portray the dynamic interplay of cytokines and growth factors in the wound milieu facilitating the repair and regeneration of cells, sculpted by the proteins. The results demonstrate the improved physical and biological properties of fabricated PASCH, depicting their synergism, and implying their competency for use in tissue engineering applications.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"25 2","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142469037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteins-Based Nanoparticles for Benznidazole Enteric Delivery 基于蛋白质的苯并咪唑肠道给药纳米颗粒

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-10-18 DOI: 10.1002/mabi.202400338

Victor A. Pilicita, Ana S. Sonzogni, Mariana Allasia, Florencia Borra, Roque J. Minari, Verónica D.G. Gonzalez

引用次数: 0

Exploiting the Potential of Decellularized Extracellular Matrix (ECM) in Tissue Engineering: A Review Study 在组织工程中开发脱细胞细胞外基质 (ECM) 的潜力：回顾研究。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-10-16 DOI: 10.1002/mabi.202400322

Peiman Brouki Milan, Farimah Masoumi, Esmaeil Biazar, Saeedeh Zare Jalise, Arezou Mehrabi

{"title":"Exploiting the Potential of Decellularized Extracellular Matrix (ECM) in Tissue Engineering: A Review Study","authors":"Peiman Brouki Milan, Farimah Masoumi, Esmaeil Biazar, Saeedeh Zare Jalise, Arezou Mehrabi","doi":"10.1002/mabi.202400322","DOIUrl":"10.1002/mabi.202400322","url":null,"abstract":"While significant progress has been made in creating polymeric structures for tissue engineering, the therapeutic application of these scaffolds remains challenging owing to the intricate nature of replicating the conditions of native organs and tissues. The use of human-derived biomaterials for therapeutic purposes closely imitates the properties of natural tissue, thereby assisting in tissue regeneration. Decellularized extracellular matrix (dECM) scaffolds derived from natural tissues have become popular because of their unique biomimetic properties. These dECM scaffolds can enhance the body's ability to heal itself or be used to generate new tissues for restoration, expanding beyond traditional tissue transfers and transplants. Enhanced knowledge of how ECM scaffold materials affect the microenvironment at the injury site is expected to improve clinical outcomes. In this review, recent advancements in dECM scaffolds are explored and relevant perspectives are offered, highlighting the development and application of these scaffolds in tissue engineering for various organs, such as the skin, nerve, bone, heart, liver, lung, and kidney.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"25 1","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142469039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnetically Active Bicontinuous Polymer Structures for Multiple Controlled Drug Delivery 用于多重可控给药的磁活性双连续聚合物结构

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-10-15 DOI: 10.1002/mabi.202470025

Elisa Lacroce, Fabio Pizzetti, Nicolás M. Barbosa Urrego, Giuseppe Nunziata, Maurizio Masi, Filippo Rossi

引用次数: 0