Macromolecular bioscience最新文献_第10页

Modeling 3D Tumor Invasiveness to Modulate Macrophage Phenotype in a Human-Based Hydrogel Platform 在人基水凝胶平台中模拟三维肿瘤侵袭性以调节巨噬细胞表型

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-06-28 DOI: 10.1002/mabi.202400227

Cátia F. Monteiro, Catarina R. Almeida, Catarina A. Custódio, João F. Mano

{"title":"Modeling 3D Tumor Invasiveness to Modulate Macrophage Phenotype in a Human-Based Hydrogel Platform","authors":"Cátia F. Monteiro, Catarina R. Almeida, Catarina A. Custódio, João F. Mano","doi":"10.1002/mabi.202400227","DOIUrl":"10.1002/mabi.202400227","url":null,"abstract":"The immune system is a pivotal player in determining tumor fate, contributing to the immunosuppressive microenvironment that supports tumor progression. Considering the emergence of biomaterials as promising platforms to mimic the tumor microenvironment, human platelet lysate (PLMA)-based hydrogel beads are proposed as 3D platforms to recapitulate the tumor milieu and recreate the synergistic tumor-macrophage communication. Having characterized the biomaterial-mediated pro-regenerative macrophage phenotype, an osteosarcoma spheroid encapsulated into a PLMA hydrogel bead is explored to study macrophage immunomodulation through paracrine signaling. The culture of PLMA-Tumor beads on the top of a 2D monolayer of macrophages reveals that tumor cells triggered morphologic and metabolic adaptations in macrophages. The cytokine profile, coupled with the upregulation of gene and protein anti-inflammatory biomarkers clearly indicates macrophage polarization toward an M2-like phenotype. Moreover, the increased gene expression of chemokines identified as pro-tumoral environmental regulators suggest a tumor-associated macrophage phenotype, exclusively stimulated by tumor cells. This pro-tumoral microenvironment is also found to enhance tumor invasiveness ability and proliferation. Besides providing a robust in vitro immunomodulatory tumor model that faithfully recreates the tumor-macrophage interplay, this human-based platform has the potential to provide fundamental insights into immunosuppressive signaling and predict immune-targeted response.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"24 11","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing the Regenerative Potential of Purified Bovine Dental Pulp and Dentin Extracellular Matrices in a Chitosan/Alginate Hydrogel 标题：利用壳聚糖/海藻酸盐水凝胶中纯化的牛牙髓和牙本质细胞外基质的再生潜力

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-06-27 DOI: 10.1002/mabi.202400254

Maree L. Gould, Nerida J. Downes, Adele G. Woolley, Haizal M. Hussaini, Jithendra T. Ratnayake, Mohammad Azam Ali, Lara T. Friedlander, Paul R. Cooper

{"title":"Harnessing the Regenerative Potential of Purified Bovine Dental Pulp and Dentin Extracellular Matrices in a Chitosan/Alginate Hydrogel","authors":"Maree L. Gould, Nerida J. Downes, Adele G. Woolley, Haizal M. Hussaini, Jithendra T. Ratnayake, Mohammad Azam Ali, Lara T. Friedlander, Paul R. Cooper","doi":"10.1002/mabi.202400254","DOIUrl":"10.1002/mabi.202400254","url":null,"abstract":"When a tooth is diseased or damaged through caries, bioactive molecules are liberated from the pulp and dentin as part of the natural response to injury and these are key molecules for stimulating stem cell responses for tissue repair. Incorporation of these extracellular-matrix (ECM)-derived molecules into a hydrogel model can mimic in vivo conditions to enable dentin–pulp complex regeneration. Here, a chitosan/alginate (C/A) hydrogel is developed to sequester bovine ECM extracts. Human dental pulp cells (hDPCs) are cultured with these constructs and proliferation and cytotoxicity assays confirm that these C/A hydrogels are bioactive. Sequential z-axis fluorescent imaging visualizes hDPCs protruding into the hydrogel as it degraded. Alizarin red S staining shows that hDPCs cultured with the hydrogels display increased calcium-ion deposition, with dentin ECM stimulating the highest levels. Alkaline phosphatase activity is increased, as is expression of transforming growth factor-beta as demonstrated using immunocytochemistry. Directional analysis following phase contrast kinetic image capture demonstrates that both dentin and pulp ECM molecules act as chemoattractants for hDPCs. Data from this study demonstrate that purified ECM from dental pulp and dentin when delivered in a C/A hydrogel stimulates dental tissue repair processes in vitro.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"24 11","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202400254","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic Effect of Multilayered Alginate/Poly(SBMA) Coatings on Marine Antifouling Property 多层海藻酸盐/聚（SBMA）涂料对海洋防污性能的协同效应

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-06-24 DOI: 10.1002/mabi.202400130

Jinwoo Lee, Inho Kim, Sung Min Kang

引用次数: 0

Mechanochemical Tailoring of Lignin Structure: Influence of Different Particle Sizes in the Organosolv Process 木质素结构的机械化学定制：有机溶胶工艺中不同颗粒尺寸的影响。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-06-20 DOI: 10.1002/mabi.202400090

Jonas Bergrath, Franz Zeppetzauer, Jessica Rumpf, Birgit Kamm, Robert Putz, Hans-Willi Kling, Margit Schulze

{"title":"Mechanochemical Tailoring of Lignin Structure: Influence of Different Particle Sizes in the Organosolv Process","authors":"Jonas Bergrath, Franz Zeppetzauer, Jessica Rumpf, Birgit Kamm, Robert Putz, Hans-Willi Kling, Margit Schulze","doi":"10.1002/mabi.202400090","DOIUrl":"10.1002/mabi.202400090","url":null,"abstract":"The autocatalyzed ethanolic organosolv process is gaining increasing attention for the sulfur-free isolation of lignin, which is subsequently used as a renewable substitute for various fossil-based applications. For the first time, the mechanochemical influence of seven different particle sizes of two different biomasses on the respective organosolv lignin structure is examined. Wine pruning (Pinot Noir) and wine pomace (Accent) are used for organosolv process with particle sizes ranging from 2.0–1.6 mm to less than 0.25 mm. As particle size decreases, the weight-average molecular weight increases, while the total phenol content decreases significantly. Additionally, the distribution of the lignin-typical monolignols and relevant substructures, as determined by two-dimensional heteronuclear nuclear magnetic resonance spectra single quantum coherence (HSQC), is observed. The degree of grinding of the biomass has a clear chemical–structural influence on the isolated HG and HGS organosolv lignins. Therefore, it is crucial to understand this influence to apply organosolv lignins in a targeted manner. In the future, particle size specifications in the context of the organosolv process should be expressed in terms of distribution densities rather than in terms of a smaller than specification.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"24 9","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202400090","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141427166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking Novel Anticancer Strategies: Bioactive Hydrogels for Local Delivery of Plasma-Derived Oxidants in an In Ovo Cancer Model 开启新的抗癌策略：生物活性水凝胶用于在卵内癌症模型中局部输送血浆氧化剂

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-06-20 DOI: 10.1002/mabi.202400213

Albert Espona-Noguera, Milica Živanić, Evelien Smits, Annemie Bogaerts, Angela Privat-Maldonado, Cristina Canal

{"title":"Unlocking Novel Anticancer Strategies: Bioactive Hydrogels for Local Delivery of Plasma-Derived Oxidants in an In Ovo Cancer Model","authors":"Albert Espona-Noguera, Milica Živanić, Evelien Smits, Annemie Bogaerts, Angela Privat-Maldonado, Cristina Canal","doi":"10.1002/mabi.202400213","DOIUrl":"10.1002/mabi.202400213","url":null,"abstract":"Cold atmospheric plasma (CAP) is a tool with the ability to generate reactive oxygen and nitrogen species (RONS), which can induce therapeutic effects like disinfection, wound healing, and cancer treatment. In the plasma oncology field, CAP-treated hydrogels (PTHs) are being explored for the local administration of CAP-derived RONS as a novel anticancer approach. PTHs have shown anticancer effects in vitro, however, they have not yet been studied in more relevant cancer models. In this context, the present study explores for the first time the therapeutic potential of PTHs using an advanced in ovo cancer model. PTHs composed of alginate (Alg), gelatin (Gel), Alg/Gel combination, or Alg/hyaluronic acid (HA) combination are investigated. All embryos survived the PTHs treatment, suggesting that the in ovo model could become a time- and cost-effective tool for developing hydrogel-based anticancer approaches. Results revealed a notable reduction in CD44+ cell population and their proliferative state for the CAP-treated Alg-HA condition. Moreover, the CAP-treated Alg-HA formulation alters the extracellular matrix composition, which may help combat drug-resistance. In conclusion, the present study validates the utility of in ovo cancer model for PTHs exploration and highlights the promising potential of Alg-based PTHs containing HA and CAP-derived RONS for cancer treatment.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"24 11","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202400213","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141427167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

pH-Responsive Degradable Electro-Spun Nanofibers Crosslinked via Boronic Ester Chemistry for Smart Wound Dressings 通过硼酸酯化学交联的 pH 值响应型可降解电纺纳米纤维用于智能伤口敷料

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-06-20 DOI: 10.1002/mabi.202400217

Sofia Nieves Casillas-Popova, Nishadi Dilkushi Lokuge, Brandon Andrade-Gagnon, Farhan Rahman Chowdhury, Cameron D. Skinner, Brandon L. Findlay, Jung Kwon Oh

{"title":"pH-Responsive Degradable Electro-Spun Nanofibers Crosslinked via Boronic Ester Chemistry for Smart Wound Dressings","authors":"Sofia Nieves Casillas-Popova, Nishadi Dilkushi Lokuge, Brandon Andrade-Gagnon, Farhan Rahman Chowdhury, Cameron D. Skinner, Brandon L. Findlay, Jung Kwon Oh","doi":"10.1002/mabi.202400217","DOIUrl":"10.1002/mabi.202400217","url":null,"abstract":"Recent advances in the treatment of chronic wounds have focused on the development of effective strategies for cutting-edge wound dressings based on nanostructured materials, particularly biocompatible poly(vinyl alcohol) (PVA)-based electro-spun (e-spun) nanofibers. However, PVA nanofibers need to be chemically crosslinked to ensure their dimensional stability in aqueous environment and their capability to encapsulate bioactive molecules. Herein, a robust approach for the fabrication of pH-degradable e-spun PVA nanofibers crosslinked with dynamic boronic ester (BE) linkages through a coupling reaction of PVA hydroxyl groups with the boronic acid groups of a phenyl diboronic acid crosslinker is reported. This comprehensive analysis reveals the importance of the mole ratio of boronic acid to hydroxyl group for the fabrication of well-defined BE-crosslinked fibrous mats with not only dimensional stability but also the ability to retain uniform fibrous form in aqueous solutions. These nanofibers degrade in both acidic and basic conditions that mimic wound environments, leading to controlled/enhanced release of encapsulated antimicrobial drug molecules. More importantly, drug-loaded BE-crosslinked fibers show excellent antimicrobial activities against both Gram-positive and Gram-negative bacteria, suggesting that this approach of exploring dynamic BE chemistry is amenable to the development of smart wound dressings with controlled/enhanced drug release.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"24 10","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202400217","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141523050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Avastin-Loaded 3D-Printed Alginate Scaffold as an Effective Antiadhesive Barrier to Prevent Postsurgical Adhesion Bands Formation 阿瓦斯汀负载的三维打印藻酸盐支架是防止术后粘连带形成的有效抗粘连屏障

IF 4.6 4区医学

Macromolecular bioscience Pub Date : 2024-06-14 DOI: 10.1002/mabi.202470014

Saeid Vakilian, Sulaiman Al-Hashmi, Juhaina Al-kindi, Fahad Al-Fahdi, Nasar Al-Wahaibi, Asem Shalaby, Hamad Al-Riyami, Ahmed Al-Harrasi, Fatemeh Jamshidi-adegani

引用次数: 0

Masthead: Macromol. Biosci. 6/2024 刊头：Macromol.Biosci.6/2024

IF 4.6 4区医学

Macromolecular bioscience Pub Date : 2024-06-14 DOI: 10.1002/mabi.202470015

引用次数: 0

3D Puzzle at the Nanoscale–How do RNA Viruses Self-Assemble their Capsids into Perfectly Ordered Structures 纳米尺度上的三维难题--RNA 病毒如何将其外壳自我组装成完美有序的结构？

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-06-12 DOI: 10.1002/mabi.202400088

Martyna Kordys, Anna Urbanowicz

{"title":"3D Puzzle at the Nanoscale–How do RNA Viruses Self-Assemble their Capsids into Perfectly Ordered Structures","authors":"Martyna Kordys, Anna Urbanowicz","doi":"10.1002/mabi.202400088","DOIUrl":"10.1002/mabi.202400088","url":null,"abstract":"The phenomenon of RNA virus self-organization, first observed in the mid-20th century in tobacco mosaic virus, is the subject of extensive research. Efforts to comprehend this process intensify due to its potential for producing vaccines or antiviral compounds as well as nanocarriers and nanotemplates. However, direct observation of the self-assembly is hindered by its prevalence within infected host cells. One of the approaches involves in vitro and in silico research using model viruses featuring a ssRNA(+) genome enclosed within a capsid made up of a single type protein. While various pathways are proposed based on these studies, their relevance in vivo remains uncertain. On the other hand, the development of advanced microscopic methods provide insights into the events within living cells, where following viral infection, specialized compartments form to facilitate the creation of nascent virions. Intriguingly, a growing body of evidence indicates that the primary function of packaging signals in viral RNA is to effectively initiate the virion self-assembly. This is in contrast to earlier opinions suggesting a role in marking RNA for encapsidation. Another noteworthy observation is that many viruses undergo self-assembly within membraneless liquid organelles, which are specifically induced by viral proteins.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"24 9","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141306260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond the Charge: Interplay of Nanogels’ Functional Group and Zeta-Potential for Antifungal Drug Delivery to Human Pathogenic Fungus Aspergillus Fumigatus 超越电荷：纳米凝胶的功能基团与 Zeta 电位在向人类致病真菌曲霉菌输送抗真菌药物中的相互作用。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-06-08 DOI: 10.1002/mabi.202400082

Theresa Vogel, Simon Kohlmann, Zahraa Abboud, Sina Thusek, Franziska Fella, Joerg Teßmar, Kazuhisa Sekimizu, Atsushi Miyashita, Andreas Beilhack, Jürgen Groll, Yidong Yu, Krystyna Albrecht

{"title":"Beyond the Charge: Interplay of Nanogels’ Functional Group and Zeta-Potential for Antifungal Drug Delivery to Human Pathogenic Fungus Aspergillus Fumigatus","authors":"Theresa Vogel, Simon Kohlmann, Zahraa Abboud, Sina Thusek, Franziska Fella, Joerg Teßmar, Kazuhisa Sekimizu, Atsushi Miyashita, Andreas Beilhack, Jürgen Groll, Yidong Yu, Krystyna Albrecht","doi":"10.1002/mabi.202400082","DOIUrl":"10.1002/mabi.202400082","url":null,"abstract":"The ubiquitous mold Aspergillus fumigatus (A. fumigatus) is one of the main fungal pathogens causing invasive infections in immunocompromised humans. Conventional antifungal agents exhibit limited efficacy and often cause severe side effects. Nanoparticle-based antifungal delivery provides a promising alternative, which can increase local drug concentration; while, mitigating toxicity, thereby enhancing treatment efficacy. Previous research underscores the potential of poly(glycidol)-based nanogels (NG) with negative surface charge as carriers for delivering antifungals to A. fumigatus hyphae. In this study, NG is tailored with 2-carboxyethyl acrylate (CEA) or with phosphoric acid 2-hydroxyethyl acrylate (PHA). It is discovered that quenching with PHA clearly improves the adhesion of NG to hyphal surface and the internalization of NG into the hyphae under protein-rich conditions, surpassing the outcomes of non-quenched and CEA-quenched NG. This enhancement cannot be solely attributed to an increase in negative surface charge but appears to be contingent on the functional group of the quencher. Further, it is demonstrated that itraconazole-loaded, PHA-functionalized nanogels (NGxPHA-ITZ) show lower MIC in vitro and superior therapeutic effect in vivo against A. fumigatus compared to pure itraconazole. This confirms NGxPHA as a promising antifungal delivery system.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"24 9","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202400082","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141288255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0