A Novel 3D Bioprinting Crosslinking Method Based on Solenoid Valve Control

Abstract

The crosslinking method of bioinks is essential for scaffold formation in 3D bioprinting. Currently, the crosslinking process of bioinks presents challenges in control, resulting in diminished stability and reliability of the gel and the presence of residual crosslinking agents that may adversely affect cell viability within the gel. This study utilizes sodium alginate as the printing ink and calcium chloride as the crosslinking agent, employing a dual-mode 3D bioprinter for alternating printing. A crosslinking agent is injected through a solenoid valve after using an extrusion-based printing method to create multilayer cell scaffolds. By controlling the printing intervals and opening times of the valve, precise localized crosslinking is achieved, and multiple alternating prints can be performed according to the required thickness of the scaffold. The results indicate that this solenoid valve crosslinking technology significantly enhances the stability and biological properties of the scaffolds, including excellent hydrophilicity, decreased swelling rate, slow degradation rate, and improved mechanical properties. Additionally, due to the reduced residual crosslinking agent, the cell proliferation rate has significantly increased. This technology advances 3D bioprinting toward a more mature stage and provides significant implications for the development of dual-mode printing.