Macromolecular bioscience最新文献

Masthead: Macromol. Biosci. 9/2024

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-09-16 DOI: 10.1002/mabi.202470021

引用次数: 0

One Stone Several Birds: Self-Localizing Submicrocages With Dual Loading Points for Multifunctional Drug Delivery

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-09-16 DOI: 10.1002/mabi.202470020

Shuxuan Liu, Jifei Wang, Yong Jiang, Yao Wang, Bin Yang, Hao Li, Guofu Zhou

引用次数: 0

Unlocking the Potential: PEGylation and Molecular Weight Reduction of Ionenes for Enhanced Antifungal Activity and Biocompatibility

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-09-16 DOI: 10.1002/mabi.202470022

Jan M. Kurzyna, Rafał J. Kopiasz, Martyna Paul, Magdalena Flont, Patrycja Baranowska, Jolanta Mierzejewska, Karolina Drężek, Waldemar Tomaszewski, Elżbieta Jastrzębska, Dominik Jańczewski

引用次数: 0

Phytotherapeutic Hierarchical PCL‐Based Scaffolds as a Multifunctional Wound Dressing: Combining 3D Printing and Electrospinning 以植物疗法为基础的分层 PCL 支架作为多功能伤口敷料：三维打印与电纺丝的结合

IF 4.6 4区医学

Macromolecular bioscience Pub Date : 2024-09-10 DOI: 10.1002/mabi.202400253

Irem Unalan, Benedikt Slavik, Andrea Buettner, Aldo R. Boccaccini

{"title":"Phytotherapeutic Hierarchical PCL‐Based Scaffolds as a Multifunctional Wound Dressing: Combining 3D Printing and Electrospinning","authors":"Irem Unalan, Benedikt Slavik, Andrea Buettner, Aldo R. Boccaccini","doi":"10.1002/mabi.202400253","DOIUrl":"https://doi.org/10.1002/mabi.202400253","url":null,"abstract":"This study focuses on developing hybrid scaffolds incorporating phytotherapeutic agents via a combination of three‐dimensional (3D) printing and electrospinning to enhance mechanical properties and provide antibacterial activity, in order to address the limitations of traditional antibiotics. In this regard, 3D‐printed polycaprolactone (PCL) struts are first fabricated using fused deposition modeling (FDM). Then, alkaline surface treatment is applied to improve the adhesion of electrospun nanofibers. Finally, peppermint oil (PEP) or clove oil (CLV)‐incorporated PCL‐gelatin (GEL) electrospun nanofibers are collected on top of the 3D‐printed PCL scaffolds by electrospinning. Incorporating PEP or CLV into PCL‐GEL electrospun nanofibers enhances the scaffold's layer detachment and adhesion force. In addition, the DPPH free radical scavenging activity assay indicates that incorporating PEP or CLV improves the antioxidant properties of the scaffolds. Further, antibacterial activity results reveal that PEP or CLV incorporated scaffolds exhibit inhibition against Staphylococcus aureus and Escherichia coli bacteria. Moreover, anti‐inflammatory assays show that scaffolds reduce the concentration of nitric oxide (NO) released from Raw 264.7 macrophage‐like cells. On the other hand, the phytotherapeutic hierarchical scaffolds have no toxic effect on normal human dermal fibroblast (NHDF) cells, and PEP or CLV enhance cell attachment and proliferation. Overall, incorporating natural phytotherapeutic agents into hierarchical scaffolds shows promise for advancing wound healing applications.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142182648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metals at the Helm: Revolutionizing Protein Assembly and Applications. 金属掌舵：彻底改变蛋白质组装和应用。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-09-06 DOI: 10.1002/mabi.202400126

Maoping Duan, Chenyan Lv, Jiachen Zang, Xiaojing Leng, Guanghua Zhao, Tuo Zhang

{"title":"Metals at the Helm: Revolutionizing Protein Assembly and Applications.","authors":"Maoping Duan, Chenyan Lv, Jiachen Zang, Xiaojing Leng, Guanghua Zhao, Tuo Zhang","doi":"10.1002/mabi.202400126","DOIUrl":"https://doi.org/10.1002/mabi.202400126","url":null,"abstract":"Protein assembly is an essential process in biological systems, where proteins self-assemble into complex structures with diverse functions. Inspired by the exquisite control over protein assembly in nature, scientists have been exploring ways to design and assemble protein structures with precise control over their topologies and functions. One promising approach for achieving this goal is through metal coordination, which utilizes metal-binding motifs to mediate protein-protein interactions and assemble protein complexes with controlled stoichiometry and geometry. Metal coordination provides a modular and tunable approach for protein assembly and de novo structure design, where the metal ion acts as a molecular glue that holds the protein subunits together in a specific orientation. Metal-coordinated protein assemblies have shown great potential for developing functional metalloproteinase, novel biomaterials and integrated drug delivery systems. In this review, an overview of the recent advances in protein assemblies benefited from metal coordination is provided, focusing on various protein arrangements in different dimensions including protein oligomers, protein nanocage and higher-order protein architectures. Moreover, the key metal-binding motifs and strategies used to assemble protein structures with precise control over their properties are highlighted. The potential applications of metal-mediated protein assemblies in biotechnology and biomedicine are also discussed.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142140490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bone-Mimetic Osteon Microtopographies on Poly-ε-Caprolactone Enhance the Osteogenic Potential of Human Mesenchymal Stem Cells. 聚ε-己内酯上的仿骨骨微结构可增强人间质干细胞的成骨潜能

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-09-05 DOI: 10.1002/mabi.202400311

Matthias Vostatek, Elettra Verin, Marvin Tamm, Mario Rothbauer, Stefan Toegel, Francesco Moscato

{"title":"Bone-Mimetic Osteon Microtopographies on Poly-ε-Caprolactone Enhance the Osteogenic Potential of Human Mesenchymal Stem Cells.","authors":"Matthias Vostatek, Elettra Verin, Marvin Tamm, Mario Rothbauer, Stefan Toegel, Francesco Moscato","doi":"10.1002/mabi.202400311","DOIUrl":"https://doi.org/10.1002/mabi.202400311","url":null,"abstract":"The attributes of implant surfaces are pivotal for successful osseointegration. Among surface engineering strategies, microtopography stands out as a promising approach to promote early cellular interactions. This study aims to design and craft a novel biomimetic osteon-like surface modification and to compare its impact on human mesenchymal stem cells (hMSCs) with four established topographies: blank, inverted pyramids, protrusions, and grooves. Poly-ε-caprolactone samples are fabricated using 2-photon-polymerization and soft lithography, prior to analysis via scanning electron microscopy (SEM), water contact angle (WCA), and protein adsorption assays. Additionally, cellular responses including cell attachment, proliferation, morphology, cytoskeletal organization, and osteogenic differentiation potential are evaluated. SEM confirms the successful fabrication of microtopographies, with minimal effect on WCA and protein adsorption. Cell attachment experiments demonstrate a significant increase on the osteon-like structure, being three times higher than on the blank. Proliferation assays indicate a fourfold increase with osteon-like microtopography compared to the blank, while ALP activity is notably elevated with osteon-like microtopography at days 7 (threefold increase over blank) and 14 (fivefold increase over blank). In conclusion, the novel biomimetic osteon-like structure demonstrates favorable responses from hMSCs, suggesting potential for promoting successful implant integration in vivo.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alginate Capsules: Versatile Applications and Production Techniques. 藻酸盐胶囊：多种应用和生产技术。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-09-05 DOI: 10.1002/mabi.202400202

Francesca Passannanti, Marianna Gallo, Giulia Lentini, Rosa Colucci Cante, Federica Nigro, Roberto Nigro, Andrea Budelli

引用次数: 0

The Effect of Immobilization Methods of P9-4 Antimicrobial Peptide Onto Gelatin Methacrylate on Multidrug-Resistant Bacteria: A Comparative Study. 在甲基丙烯酸明胶上固定 P9-4 抗菌肽的方法对耐多药细菌的影响：比较研究

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-09-04 DOI: 10.1002/mabi.202400324

Günnur Pulat, Nisa Nilsu Çelebi, Eda Bilgiç

{"title":"The Effect of Immobilization Methods of P9-4 Antimicrobial Peptide Onto Gelatin Methacrylate on Multidrug-Resistant Bacteria: A Comparative Study.","authors":"Günnur Pulat, Nisa Nilsu Çelebi, Eda Bilgiç","doi":"10.1002/mabi.202400324","DOIUrl":"https://doi.org/10.1002/mabi.202400324","url":null,"abstract":"Wound dressings play a crucial role in wound management by providing a protective barrier and creating an optimal environment for healing. Photocrosslinkable hydrogels, such as gelatin methacrylate (GelMA), have gained attention for their unique properties but often lack antimicrobial activity. To enhance their effectiveness, researchers are exploring methods to incorporate antimicrobial agents into photocrosslinkable hydrogel dressings. Immobilization of antimicrobial peptides (AMPs) onto hydrogel matrices may be achieved through physical or chemical methods. Although, chemical immobilization, using techniques like EDC/NHS chemistry, has shown promise in enhancing antimicrobial properties of hydrogels, the capacity for immobilization may be limited by the structure of hydrogel. Physical methods, such as immersing, offer alternatives but may have different efficacy and biocompatibility. The study aims to chemically immobilize GelMA with P9-4 AMP by photoinduced conjugation and EDC/NHS chemistry and compare its antimicrobial efficacy with a physical immobilization method. Chemical immobilization by EDC/NHS chemistry significantly enhances the antimicrobial effect of GelMA hydrogels against multi-drug resistant Psuedomonas aeruginosa (MDR P. aeruginosa) and methicillin-resistant Staphylococcus aureus (MRSA) while maintaining favorable biocompatibility. Study highlights the potential of AMP-functionalized GelMA as advanced wound dressings for reducing infections caused by antibiotic-resistant bacteria and offers a promising approach for future research in wound management.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142126128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial Cellulose as a UVB Filter to Protect the Skin Microbiota. 细菌纤维素作为 UVB 过滤器保护皮肤微生物群。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-09-03 DOI: 10.1002/mabi.202400269

Pablo Alarcón-Guijo, Víctor Garcés, Ana González, José M Delgado-López, Ruh Ullah, Vipul Bansal, Jose M Dominguez-Vera

{"title":"Bacterial Cellulose as a UVB Filter to Protect the Skin Microbiota.","authors":"Pablo Alarcón-Guijo, Víctor Garcés, Ana González, José M Delgado-López, Ruh Ullah, Vipul Bansal, Jose M Dominguez-Vera","doi":"10.1002/mabi.202400269","DOIUrl":"https://doi.org/10.1002/mabi.202400269","url":null,"abstract":"Certain aerobic bacteria produce bacterial cellulose (BC) to protect themselves from UV radiation. Inspired by this natural function, the UV-filtering capacity of wet BC film (BC) and dried BC (BC-Dried) is evaluated and it is concluded that both samples hardly filter UVA, but filter UVB to some extent, especially BC-Dried. Moreover, this filtering capacity does not diminish but significantly increases with time, with efficiencies in the 145-160 min time range equal to or greater than most UV filters of the market. This increase in efficiency is due to the fact that the BC structure is modified by prolonged exposure to UVB radiation. Specifically, UVB causes sintering of the cellulose fibers, making the structure denser and increasing its reflection and scattering of UVB radiation. Remarkably, this UVB filtering ability of BC allows it to protect key skin probiotics, Lactobacillus fermentum (L. fermentum) and Cutibacterium acnes (C. acnes), against UVB damage. While the protection of healthy skin microbiota is not currently a regulatory requirement for sunscreens with UV filters, it may become a key differentiator for future UV filters given the increasing evidence on the role of skin microbiota in health.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142120204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovative Fluorescent Polymers in Niosomal Carriers: A Novel Approach to Enhancing Cancer Therapy and Imaging. 创新型荧光聚合物在纳米载体中的应用：增强癌症治疗和成像的新方法。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-09-02 DOI: 10.1002/mabi.202400343

Selay Tornaci, Merve Erginer, Umut Bulut, Beste Sener, Elifsu Persilioglu, İsmail Bergutay Kalaycilar, Emine Guler Celik, Hasret Yardibi, Pinar Siyah, Oguzhan Karakurt, Ali Cirpan, Baris Gokalsin, Ahmet Murat Senisik, Firat Baris Barlas

{"title":"Innovative Fluorescent Polymers in Niosomal Carriers: A Novel Approach to Enhancing Cancer Therapy and Imaging.","authors":"Selay Tornaci, Merve Erginer, Umut Bulut, Beste Sener, Elifsu Persilioglu, İsmail Bergutay Kalaycilar, Emine Guler Celik, Hasret Yardibi, Pinar Siyah, Oguzhan Karakurt, Ali Cirpan, Baris Gokalsin, Ahmet Murat Senisik, Firat Baris Barlas","doi":"10.1002/mabi.202400343","DOIUrl":"https://doi.org/10.1002/mabi.202400343","url":null,"abstract":"Cancer is anticipated to become the pioneer reason of disease-related deaths worldwide in the next two decades, underscoring the urgent need for personalized and adaptive treatment strategies. These strategies are crucial due to the high variability in drug efficacy and the tendency of cancer cells to develop resistance. This study investigates the potential of theranostic nanotechnology using three innovative fluorescent polymers (FP-1, FP-2, and FP-3) encapsulated in niosomal carriers, combining therapy (chemotherapy and radiotherapy) with fluorescence imaging. These cargoes are assessed for their cytotoxic effects across three cancer cell lines (A549, MCF-7, and HOb), with further analysis to determine their capacity to augment the effects of radiotherapy using a Linear Accelerator (LINAC) at specific doses. Fluorescence microscopy is utilized to verify their uptake and localization in cancerous versus healthy cell lines. The results confirmed that these niosomal cargoes not only improved the antiproliferative effects of radiotherapy but also demonstrate the practical application of fluorescent polymers in in vitro imaging. This dual function underscores the importance of dose optimization to maximize therapeutic benefits while minimizing adverse effects, thereby enhancing the overall efficacy of cancer treatments.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142108936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0