Macromolecular bioscience最新文献

{"title":"Evaluation of HER2 Targeted Poly(Ethylene Glycol)-Poly(L-histidine) Copolymer Micelles in Breast Cancer Models.","authors":"Remya Valsala Kumari, Abhilash D Pandya, Solveig J Pettersen, Audun Kvalvaag, Anne Grethe Myrann, Razvan Ghiarasim, Alexandru Rotaru, Mihail-Gabriel Dimofte, Tore Skotland, Kirsten Sandvig, Gunhild Mari Mælandsmo, Tore-Geir Iversen","doi":"10.1002/mabi.202500047","DOIUrl":"https://doi.org/10.1002/mabi.202500047","url":null,"abstract":"<p><p>Breast cancer positive for human epidermal growth factor receptor-2 (HER2) is challenging to treat due to the development of drug resistance, even with recently developed antibody-mediated therapeutics. However, nanotechnology provides new innovative therapeutic strategies. This study evaluates the efficacy of new HER2-targeted, pH-sensitive poly(ethylene glycol)-poly(L-histidine) copolymer micelles (PEG-PHis micelles), either empty, loaded with the drug doxorubicin (DOX), or the fluorophore Rhodamine-B (RB) for imaging purposes. Cellular association of the micelles, in vitro effects on proliferation and cytotoxicity, as well as ex vivo and in vivo responses on tumor growth are assessed. The results indicate that trastuzumab (TZM) conjugation enhances the specific cellular association of PEG-PHis micelles in HER2 overexpressing cells and improves the cytotoxic effects of the micelles themselves. Although the TZM conjugation of DOX-loaded micelles did not contribute to an enhancement in overall efficacy in cell lines and tumor explant cultures, the findings highlight the potential of TZM conjugation on these micelles in targeting HER2-positive tumors.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00047"},"PeriodicalIF":4.1,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144732075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research Progress on Antimicrobial Biomaterials.","authors":"Ruirui Zhang, Hao Lin, Ze Wang, Shujie Cheng, Chong Li, Yi Wang","doi":"10.1002/mabi.202500239","DOIUrl":"https://doi.org/10.1002/mabi.202500239","url":null,"abstract":"<p><p>Bacterial infections and biofilm-associated complications pose significant global health challenges, exacerbated by antibiotic overuse and multidrug-resistant strains. In this review, we commence by delving into the processes of bacterial adhesion and biofilm formation. Subsequently, we elaborate on the mechanisms underlying passive antibacterial adhesion-resistant surfaces and active sterilization materials. Finally, we introduce the application potential of multi-mechanism synergistic and intelligent-responsive antimicrobial materials in the biomedical field. This study systematically explores the design principles and performance optimization strategies of antimicrobial materials. It offers theoretical guidance for the development of a new generation of antimicrobial materials with improved efficacy and safety.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00239"},"PeriodicalIF":4.4,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144715144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Natural Polyphenol-Regulated Microporous Soybean Protein Injectable Hydrogel Loaded with Placental Stem Cell-Derived Exosomes for Promoting Chronic Wound Healing.","authors":"Hui Liu, Wen Jiang, Lu Han, Xiong Lu","doi":"10.1002/mabi.202400546","DOIUrl":"https://doi.org/10.1002/mabi.202400546","url":null,"abstract":"<p><p>Diabetic chronic wounds exhibit difficulties in healing due to prolonged high glucose and inflammatory conditions. Suppression of inflammation and restoration of the epithelial layer are crucial for preventing infections, reducing moisture loss, and restoring structural and functional integrity to the wound. Currently, there are numerous studies on dressings for diabetic chronic wounds, most of which can inhibit inflammation, but the effect on epithelial regeneration is limited. In this study, we developed a novel therapeutic system utilizing a microporous hydrogel regulated by the natural tea polyphenol EGCG for the treatment of chronic wounds. This hydrogel, made from soy protein isolate and dextran, exhibits anti-inflammatory properties and excellent biocompatibility, and is designed to be both injectable and biodegradable. This hydrogel facilitated the efficient loading and release of human placental stem cell-derived exosomes. Then the exosome-loaded hydrogel was used for full-thickness diabetic skin wound healing. Histological characterization confirmed that the exosome-loaded hydrogel promoted wound healing and epidermal regeneration. Immunofluorescence staining and Western blot experiments demonstrated the anti-inflammatory effect of the hydrogel. In short, this study proposed an Exo-hydrogel therapy as a promising potential for effective treatment of chronic wounds.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00546"},"PeriodicalIF":4.4,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vascularized Tumor-on-a-Chip Model as a Platform for Studying Tumor-Microenvironment-Drug Interaction.","authors":"Hyelim Kim, Seung-Woo Cho, Hong Nam Kim","doi":"10.1002/mabi.202500240","DOIUrl":"https://doi.org/10.1002/mabi.202500240","url":null,"abstract":"<p><p>As cancer-targeting technologies advance, robust platforms for evaluating drug delivery systems (DDS) under pathomimetic conditions are critically needed. Traditional models inadequately mimic human tumor microenvironment (TME) complexity due to interspecies variance, structural simplification, and static perfusion. Vascularized tumor-on-a-chip systems address these gaps by integrating perfusable vasculature with tumor-stroma dynamics in microfluidic environments, enabling dynamic 3D evaluation of drug transport kinetics and therapeutic efficacy. These advances significantly enhance preclinical-to-clinical translatability, though challenges remain in achieving long-term vascular stability and multi-tissue integration under physiological flow conditions. Herein, we summarize recent progress in vascularized tumor-on-a-chip technologies for assessing DDS performance and TME interactions. Finally, opportunities for precision oncology and integrative organ-level modeling are highlighted, underscoring the transformative potential of these platforms in next-generation cancer research.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00240"},"PeriodicalIF":4.4,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DGGcm Loaded with LGG for Promoting Diabetic Infected Wound Healing.","authors":"Wuliang Diao, Weidong Li, Peiting Li, Xueyao Cai, Zhenyang Xiao, Jianda Zhou","doi":"10.1002/mabi.202500237","DOIUrl":"https://doi.org/10.1002/mabi.202500237","url":null,"abstract":"<p><p>The skin serves as the body's primary defense barrier, crucial for protection against external aggressors and maintaining stable body temperature. Diabetic patients, due to vascular and neuropathic damage induced by hyperglycemia, experience significantly impaired healing capacity, rendering them vulnerable to chronic wounds and infections, which may necessitate amputations. Therefore, investigating effective treatments that expedite wound healing in diabetic patients is of considerable clinical importance. This study evaluates the efficacy of Dextran-Gelatin-Gellan Gum composite microspheres (DGGcm) loaded with Lactobacillus rhamnosus (LGG) in the repair of full-thickness skin defects and infected wounds in diabetic rats. Uniformly shaped DGGcm were prepared using a combination of emulsification and microfluidic technology. After LGG loading, in vitro experiments-including cell live/dead staining, CCK-8 proliferation assays, migration and tubule formation evaluations, and antibacterial testing-were performed to assess the effects of DGGcm combined with LGG on cell proliferation, migration, angiogenesis, and antibacterial efficacy. Subsequently, a diabetic rat model with full-thickness skin defects and infections was established to compare the therapeutic effects of DGGcm combined with LGG against other treatment groups. Histological analysis, qRT-PCR, and Western Blot (WB) assays were utilized to evaluate tissue repair, collagen deposition, and cytokine expression. The study demonstrated that DGGcm possesses excellent biocompatibility and degradability, with LGG incorporation facilitating sustained release. In vitro experiments revealed that DGGcm combined with LGG significantly enhanced cell proliferation, migration, tubule formation, and antibacterial properties. In vivo results indicated that this combination markedly accelerated wound healing in diabetic rats. Histological analysis revealed that the DGGcm-LGG formulation closely resembled normal skin architecture, exhibiting effective tissue restoration, fiber alignment, and collagen deposition. Molecular biology analyses indicated that DGGcm combined with LGG significantly suppressed the expression of the pro-inflammatory cytokine IL-6, elevated the expression of the anti-inflammatory cytokine IL-10, and promoted the expression of critical wound healing proteins, including CD31, KI-67, MMP-2, TGF-β, VEGF, and α-SMA. This study successfully developed DGGcm with exceptional biocompatibility and degradability, effectively loading LGG to achieve sustained release. The DGGcm-LGG combination significantly enhances cell proliferation, migration, tubule formation, and antibacterial efficacy, thereby promoting the healing of infected wounds in diabetic rats. These findings propose a novel therapeutic strategy with substantial clinical application potential for wound repair in diabetic patients.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00237"},"PeriodicalIF":4.4,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144675195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Permeation and Release Behavior Based on Structural Differences in the Gelatin Network within Hydrogels.","authors":"Tamaki Maeda, Satsuki Tajima, Miho Suto, Kazuki Murai","doi":"10.1002/mabi.202400628","DOIUrl":"https://doi.org/10.1002/mabi.202400628","url":null,"abstract":"<p><p>Living organisms exhibit unique functionalities through reversible structural transitions of biomacromolecular assemblies, enabling molecular recognition and selective permeability. Inspired by these systems, we investigated anisotropic and isotropic gelatin hydrogels as models to mimic the structural transitions of biological channels. Using a template-based method, anisotropic gelatin networks were formed with polypropylene and polyvinyl chloride templates, while isotropic networks were fabricated on glass substrates. Permeability studies with model molecules (phenylalanine, methylene blue, and rhodamine B) demonstrated that molecular properties (compound's balance between hydrophobicity and hydrophilicity) influenced transport behaviors, highlighting structural dependency. Mineralization experiments further validated the hydrophobic regions within anisotropic hydrogels, promoting silica formation while restricting calcium phosphate deposition. Additionally, drug release studies indicated anisotropic hydrogels preferentially released hydrophobic molecules, while isotropic hydrogels favored hydrophilic drugs. These findings elucidate the role of network anisotropy in the functions of hydrogel and provide insights into designing bioinspired functional materials for applications such as drug delivery systems and biomimetic membranes.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00628"},"PeriodicalIF":4.4,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural Medicine and 3D Printing for Tissue Repair, Drug Delivery, and Wound Healing: Integrating Traditional Chinese Medicine Bio-Actives with Advanced Biomaterials.","authors":"Fatima Kanwal, Abdullah A Al-Almadi, Maryam Khurshid, Shuo Chen, Chuanglong He","doi":"10.1002/mabi.202500173","DOIUrl":"https://doi.org/10.1002/mabi.202500173","url":null,"abstract":"<p><p>Integrating Traditional Chinese Medicine (TCM) bio-actives in 3D-printed scaffolds combines ancient pharmacology with advanced additive manufacturing strategies for bone repair, wound healing, and drug delivery. This review highlights Carthamus tinctorius for cranial angiogenesis, Radix Dipsaci for fracture healing, Pearl powder for bone-mimicking mineralization, and ginger-garlic extract-loaded osteogenic-antibacterial implant. Tailored drug releases (21 days for Nuciferine and 56 days for Berberine) and Pyritum-enhanced β-TCP scaffolds showing spatial engineering by doubling compressive strength. Oregano essential oil liposomes suppress osteosarcoma and enhance antibacterial efficacy. The review also discusses Panax notoginseng living scaffold targeting hypoxia, Cryptotanshinone niosomal hydrogel for acne, Astragalus membranaceus for treating Scleroderma diabeticorum, and Okra polysaccharides for both pharmacological and structural function, Liquidambar orientalis skin-regenerating and Bombyx mori silk fibroin skin adhesive properties. Bio-3D printing advances TCM-enriched hydrogels, improving bio-ink formulation, hydrogel stability, drug delivery, and regenerative efficacy. Each section examines TCM pharmacological compounds needed for 3D-printed scaffold integration, the scaffold polymers, and the performance validation models. The interdisciplinary approach of combining biomaterials, TCM pharmacology, and precision of 3D printing redefines regenerative paradigms. The conclusions summarize key findings, define future research trajectories, and address potential challenges with targeted solutions, offering a forward-looking perspective for this transformative interdisciplinary field.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00173"},"PeriodicalIF":4.4,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polypept(o)ide-Based Core–Shell Bottlebrush Polymers: A Versatile Platform for Drug Encapsulation","authors":"Bonan Zhao,&nbsp;Jingyuan Wei,&nbsp;Rüdiger Berger,&nbsp;Lin Jian,&nbsp;Kaloian Koynov,&nbsp;Heyang Zhang,&nbsp;Matthias Barz","doi":"10.1002/mabi.202570013","DOIUrl":"https://doi.org/10.1002/mabi.202570013","url":null,"abstract":"<p><b>Front Cover</b>: Cylindrical bottlebrush polymers (CBPs) enable precise control over nanoparticle properties via polymer synthesis. Here, a grafting-from approach utilizing a polylysine backbone enabled the formation of amphiphilic polypept(o)ide-based side chains by sequential NCA-ROP, which provides convenient access to core-shell CBPs. These core-shell architectures combine Dasatinib-loading and sustained release kinetics with cellular uptake and therapeutic efficacy in glioblastoma cells. More details can be found in article 2500083 by Heyang Zhang, Matthias Barz, and co-workers.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"25 7","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202570013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144624211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mallotus philippensis Extract-Infused Locust Bean Gum-Based Ternary Hydrogel: A Green Fabrication Approach for the Controlled Release of Levofloxacin.","authors":"Sehrish Jabeen, Atif Islam, Rafi Ullah Khan, Dirk W Schubert","doi":"10.1002/mabi.202500266","DOIUrl":"https://doi.org/10.1002/mabi.202500266","url":null,"abstract":"<p><p>Smart and responsive drug delivery systems are key to next-generation biomedical therapies, offering precision and targeted action. This study reports the development of a novel biodegradable, pH-sensitive, and highly swellable hydrogel composed of locust bean gum, polyvinylpyrrolidone, chitosan, and (3-aminopropyl)triethoxysilane (APTES) as a crosslinker, incorporating Mallotus philippensis (MP) extract to enhance bioactivity. Structural, thermal, and morphological properties were characterized by FT-IR, TGA, and SEM. Swelling behaviour confirmed pH responsiveness, while gel content and biodegradation assays verified stability and degradability. Contact angle and porosity analyses showed favourable surface wettability and porous architecture. Antimicrobial activity demonstrated inhibition of bacterial strains, with cytocompatibility supported by brine shrimp lethality assay. Levofloxacin (LVX) was loaded into hydrogels with and without MP extract, achieving drug encapsulation efficiencies of 89% and 85%, respectively, with a slight decrease attributed to phytoconstituents interactions affecting network density. Drug release profiling at pH 5.5, 6.5, and 7.4 showed sustained release exceeding 80% within 3 h per USP standards. LPC-3AT and LPC-3AT-MP 400 released 87.04% and 94.5% LVX over 180 min in PBS, following a non-Fickian (anomalous) transport mechanism (diffusion exponent n = 0.62). These findings highlight the hydrogel's promise as an injectable platform for controlled drug delivery and advanced biomedical applications.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00266"},"PeriodicalIF":4.4,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Issue Information: Macromol. Biosci. 7/2025","authors":"","doi":"10.1002/mabi.202570014","DOIUrl":"https://doi.org/10.1002/mabi.202570014","url":null,"abstract":"","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"25 7","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202570014","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144624661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}