Macromolecular bioscience最新文献

PROTAC and Molecular Glue Degraders of the Oncogenic RNA Binding Protein Lin28. 致癌 RNA 结合蛋白 Lin28 的 PROTAC 和分子胶降解剂。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-11-22 DOI: 10.1002/mabi.202400427

Aseel Kashkush, Judith Furth-Lavi, Jiri Hodon, Raphael I Benhamou

{"title":"PROTAC and Molecular Glue Degraders of the Oncogenic RNA Binding Protein Lin28.","authors":"Aseel Kashkush, Judith Furth-Lavi, Jiri Hodon, Raphael I Benhamou","doi":"10.1002/mabi.202400427","DOIUrl":"https://doi.org/10.1002/mabi.202400427","url":null,"abstract":"The interaction between proteins and RNA is crucial for regulating gene expression, with dysregulation often linked to diseases such as cancer. The RNA-binding protein (RBP) Lin28 inhibits the tumor suppressor microRNA (miRNA) let-7, making it a significant oncogenic factor in tumor progression and metastasis. In this study, a small molecule is used that binds Lin28 and blocks its inhibition of let-7. To enhance its efficay, the inhibitor is transformed into degraders via two degradation approaches: Proteolysis Targeting Chimera (PROTAC) and molecular glue. A series of PROTAC bifunctional molecules and molecular glues capable of degrading Lin28 in cells.is developed Both strategies significantly reduce overexpressed Lin28 and alleviate cancer cellular phenotypes. Notably, the molecular glue approach demonstrates exceptional potency, surpassing PROTAC in several aspects. This outcome underscores the superior efficiency of the molecular glue approach for targeted Lin28 degradation and highlights its potential for addressing associated diseases with small molecules. Innovative small molecule strategies such as molecular glue and PROTAC technology for targeted RBP degradation, hold promise for opening new avenues in RNA modulation and addressing related diseases.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2400427"},"PeriodicalIF":4.4,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142687458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustained Drug Release from Dual-Responsive Hydrogels for Local Cancer Chemo-Photothermal Therapy. 用于局部癌症化疗-光热疗法的双反应水凝胶的持续药物释放。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-11-20 DOI: 10.1002/mabi.202400413

Zhixiang Liu, Yoshitaka Koseki, Ryuju Suzuki, Anh Thi Ngoc Dao, Hitoshi Kasai

{"title":"Sustained Drug Release from Dual-Responsive Hydrogels for Local Cancer Chemo-Photothermal Therapy.","authors":"Zhixiang Liu, Yoshitaka Koseki, Ryuju Suzuki, Anh Thi Ngoc Dao, Hitoshi Kasai","doi":"10.1002/mabi.202400413","DOIUrl":"https://doi.org/10.1002/mabi.202400413","url":null,"abstract":"As an exceptional carrier for localized drug delivery to tumors, hydrogels can achieve prolonged drug release through careful design and adjustments, effectively targeting cancer cells and minimizing side effects. This study investigates a novel dual-responsive hydrogel system designed for the delivery of nanomedicines, focusing on drug release and the local antitumor efficacy of SN-38-cholesterol nanoparticles (SN-38-chol NPs) and polydopamine NPs (PDA NPs)/poly(n-isopropylacrylamide) (pNIPAM) hydrogels. By combining the thermosensitive properties of pNIPAM with the near-infrared (NIR) responsiveness of PDA NPs, the hydrogel aims to enhance on-demand drug release. SN-38-chol NPs, known for their stability and small size, are incorporated into the hydrogel to improve drug release dynamics. The investigation reveals a drug release cycle of over three weeks, maintaining sensitivity to both temperature and NIR light for controlled drug release. In vivo studies demonstrate the high tumor growth inhibition performance of the system after photothermal treatment induced by 808 nm NIR light. These results suggest that the drug-carrying hydrogel system holds promise for diverse applications in chemical and physical therapies, including the treatment of malignant wounds, post-surgery wound healing, and direct tumor treatment. This study establishes the potential of SN-38-chol NPs and PDA NPs/pNIPAM hydrogels as effective platforms for chemo-phototherapy.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2400413"},"PeriodicalIF":4.4,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Mg-Alginate Based Self Disassociative Bio-Ink for Magnetic Bio-Patterning of 3D Tumor Models. 开发基于海藻酸镁的自解离生物墨水，用于三维肿瘤模型的磁性生物图案。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-11-18 DOI: 10.1002/mabi.202400339

Basak Coban, Mehmet Baskurt, Hasan Sahin, Ahu Arslan-Yildiz

{"title":"Development of Mg-Alginate Based Self Disassociative Bio-Ink for Magnetic Bio-Patterning of 3D Tumor Models.","authors":"Basak Coban, Mehmet Baskurt, Hasan Sahin, Ahu Arslan-Yildiz","doi":"10.1002/mabi.202400339","DOIUrl":"https://doi.org/10.1002/mabi.202400339","url":null,"abstract":"Alginate forms a hydrogel via physical cross-linking with divalent cations. In literature, Ca2+ is mostly utilized due to strong interactions but additional procedures are required to disassociate Ca-alginate hydrogels. On the other hand, Mg-alginate hydrogels disassociate spontaneously, which might benefit certain applications. This study introduces Mg-alginate as the main component of a bio-ink for the first time to obtain 3D tumor models by magnetic bio-patterning technique. The bio-ink contains magnetic nanoparticles (MNPs) for magnetic manipulation, Mg-alginate hydrogel as a sacrificial material, and cells. The applicability of the methodology is tested for the formation of 3D tumor models using HeLa, SaOS-2, and SH-SY5Y cells. Long-term cultures are examined by Live/dead and MTT analysis and revealed high cell viability. Subsequently, Collagen and F-actin expressions are observed successfully in 3D tumor models. Finally, the anti-cancer drug Doxorubicin (DOX) effect is investigated on 3D tumor models, and IC50 values is calculated to assess the drug response. As a result, significantly higher drug resistance is observed for bio-patterned 3D tumor models up to tenfold compared to 2D control. Overall, Mg-alginate hydrogel is successfully used to form bio-patterned 3D tumor models, and the applicability of the model is shown effectively, especially as a drug screening platform.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2400339"},"PeriodicalIF":4.4,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of Polyvinyl Alcohol/Amoxicillin - Chitosan/Collagen Hybrid Bilayer Membranes for Regeneration of Gingival Tissues. 生产用于牙龈组织再生的聚乙烯醇/阿莫西林-壳聚糖/胶原混合双层膜

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-11-18 DOI: 10.1002/mabi.202400331

Ayca Aydin, Songul Ulag, Sabereh Nouri, Elif Durasi, Pelin Pelit Arayıcı, Gülgün Bosgelmez Tinaz, Mehmet Mücahit Güncü, Rabia Cakir, Oguzhan Gunduz, Cem Bulent Ustundag

{"title":"Production of Polyvinyl Alcohol/Amoxicillin - Chitosan/Collagen Hybrid Bilayer Membranes for Regeneration of Gingival Tissues.","authors":"Ayca Aydin, Songul Ulag, Sabereh Nouri, Elif Durasi, Pelin Pelit Arayıcı, Gülgün Bosgelmez Tinaz, Mehmet Mücahit Güncü, Rabia Cakir, Oguzhan Gunduz, Cem Bulent Ustundag","doi":"10.1002/mabi.202400331","DOIUrl":"https://doi.org/10.1002/mabi.202400331","url":null,"abstract":"Periodontal diseases, if untreated, can cause gum recession and tooth root exposure, resulting in infection and irreversible damage. Traditional treatments using autologous grafts are painful and often result in postoperative complications. Scaffolds offer a less invasive alternative, promoting cell proliferation and healing without additional surgery, thus enhancing comfort for patients and doctors. This study developed Chitosan (Chit)/Collagen (Col) film surfaces and drug-loaded Polyvinyl Alcohol (PVA)/Amoxicillin (AMX) nanofibers using solvent casting and electrospinning methods, respectively. The surfaces are characterized by scanning electron microscopy (SEM), mechanical testing, Fourier Transform Infrared Spectroscopy (FTIR), and differential scanning calorimetry (DSC). Biocompatibility and antimicrobial properties are assessed using NIH/3T3 fibroblast cells and bacterial cultures. SEM images confirmed the structural integrity of AMX-loaded 13% PVA nanofibers, while FTIR analysis validated the compositional integrity of PVA/AMX nanofibers and Chit/Col film hybrid surfaces. Cell studies showed over 90% viability for Chit/Col film + PVA/AMX nanofiber hybrid bilayer membranes, confirming their biocompatibility. The antimicrobial assessment indicated that the Chit/Col film + PVA/AMX (0.2%) nanofiber hybrid bilayer membrane exhibited superior efficacy against Streptococcus mutans. These findings suggest that this hybrid bilayer membrane can enhance cell growth, promote proliferation, and enable controlled drug release, offering significant promise for regeneration of gingival tissues.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2400331"},"PeriodicalIF":4.4,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Masthead: Macromol. Biosci. 11/2024 刊头：Macromol.Biosci.11/2024

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-11-15 DOI: 10.1002/mabi.202470027

引用次数: 0

Biochemical Signal-Induced Supramolecular Hydrogelation for Structured Free-Standing Soft Material Formation. 生化信号诱导超分子水凝胶化，形成结构化的独立软材料。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-11-15 DOI: 10.1002/mabi.202400419

Dineshkumar Bharathidasan, Akshay Sunil Salvi, Suryasarathi Bose, Chandan Maity

{"title":"Biochemical Signal-Induced Supramolecular Hydrogelation for Structured Free-Standing Soft Material Formation.","authors":"Dineshkumar Bharathidasan, Akshay Sunil Salvi, Suryasarathi Bose, Chandan Maity","doi":"10.1002/mabi.202400419","DOIUrl":"https://doi.org/10.1002/mabi.202400419","url":null,"abstract":"Cells coordinate their activity and regulate biological processes in response to chemical signals. Mimicking natural processes, control over the formation of artificial supramolecular materials is of high interest for their application in biology and medicine. Supramolecular material that can form in response to chemical signals is important for the development of autonomously responsive materials. Herein, a supramolecular hydrogel system is reported enabling in situ generation of hydrogelators in response to a specific chemical signal. Using self-immolative chemistry, spatial control over the formation of supramolecular hydrogel material and structured free-standing hydrogel objects via providing H2O2 locally is demonstrated. In addition, a hybrid system is developed enabling in situ generation of the H2O2 by the action of an enzyme and glucose, providing an extra handle for the development of an intelligent soft material. This generic design should enable the use of various (chemical)stimuli that can be obtained via coupling different stimuli and various chemical and/or biological markers and appears a versatile approach for the design of smart artificial soft materials that can find application in theranostic purposes.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2400419"},"PeriodicalIF":4.4,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142639252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanically Stable and Biocompatible Polymer Brush Coated Dental Materials with Lubricious and Antifouling Properties 具有润滑和防污特性的机械稳定且生物兼容的聚合物刷涂牙科材料

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-11-15 DOI: 10.1002/mabi.202470026

Rong Mu, Ling Yang, Xinyue Wang, Binrui Yang, Jia Li, Aijun Wang, Guorui Zhang, Chufeng Sun, Yang Wu, Bo Yu, Bin Li

引用次数: 0

In Situ Forming Injectable Gelatin-Based Antibacterial Bioadhesives for Preventing Postoperative Leakage and Abdominal Adhesions. 用于防止术后渗漏和腹部粘连的原位成型明胶基抗菌生物粘合剂。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-11-08 DOI: 10.1002/mabi.202400372

Shibo Hu, Wanglin Duan, Xianzhen Jin, Chaowei Li, Bin Zhu, Yurong Chen, Ye Zhu, Peihai Cao, Zepeng Dong, Luyao Feng, Junhui Yu, Xuejun Sun, Zeshaan Yahaya Haji Mahmood, Yazhong Bu, Baoji Du, Jianbao Zheng

{"title":"In Situ Forming Injectable Gelatin-Based Antibacterial Bioadhesives for Preventing Postoperative Leakage and Abdominal Adhesions.","authors":"Shibo Hu, Wanglin Duan, Xianzhen Jin, Chaowei Li, Bin Zhu, Yurong Chen, Ye Zhu, Peihai Cao, Zepeng Dong, Luyao Feng, Junhui Yu, Xuejun Sun, Zeshaan Yahaya Haji Mahmood, Yazhong Bu, Baoji Du, Jianbao Zheng","doi":"10.1002/mabi.202400372","DOIUrl":"https://doi.org/10.1002/mabi.202400372","url":null,"abstract":"Postoperative anastomotic leakage (AL) and abdominal adhesions are two major complications after intestinal surgery, with an incidence of 2-25% for AL and 93% for adhesion. Until now, there is no method addressing AL and abdominal adhesions simultaneously. In this work, Bi-PEG-succinimidyl succinate (PEG-NHS), amino-gelatin (Agel) is combined with cefoperazone-sulbactam (SCF) to prepare a multifunctional bioadhesive (SCF/SEAgel) for the postoperative leakage and adhesion prevention. SCF/SEAgel possesses a great sealing capability for tissue, with a bursting pressure of 54 kPa. The loaded SCF endows the systems with good antibacterial properties. The in vivo antiadhesion experiments show that SCF/SEAgel possesses better anti-adhesion properties than the commercially used sodium hyaluronate gel. In the cecum leakage model, the SCF/SEAgel effectively seals the leakage with a survival rate of 100%, superior to commercial products (Sainaoning). Meanwhile, it significantly reduces tissue adhesion. Finally, the laparoscopic surgery with dogs shows that the SCF/SEAgel can be injected through minimally invasive surgery, demonstrating its ease of use. Combined with its great biocompatibility, SCF/SEAgel is very promising in intestinal surgery.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2400372"},"PeriodicalIF":4.4,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyamino Acid Based Zwitterionic Coating can Inhibit Coagulation and Inflammation Through Anti-Fouling and Restoring Microenvironment. 聚氨基酸型聚合离子涂层可通过防污和恢复微环境来抑制凝血和炎症。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-11-08 DOI: 10.1002/mabi.202400336

Zehong Xiang, Honghong Chen, Feng Wu, Haobo Pan

{"title":"Polyamino Acid Based Zwitterionic Coating can Inhibit Coagulation and Inflammation Through Anti-Fouling and Restoring Microenvironment.","authors":"Zehong Xiang, Honghong Chen, Feng Wu, Haobo Pan","doi":"10.1002/mabi.202400336","DOIUrl":"https://doi.org/10.1002/mabi.202400336","url":null,"abstract":"Protein adhesion and thrombosis formation caused by limited surface properties pose great challenges to biomedical implants. Although various hydrophilic coating or drug release coatings are reported, the single coating cannot cope with cases under the condition of complex physiological environment, which causes the coating effect is limited. In this study, a polyamino acid-derived zwitterionic coating is constructed to eliminate reactive oxygen species (ROS) in the microenvironment. It is demonstrated that the coating has excellent hydrophilicity, stability, and lubricity, and can obviously prevent protein adhesion. At the same time, the coating can eliminate hydrogen peroxide and maintain the stability of the microenvironment. The in vivo and in vitro experiments show that the coating has good biocompatibility, and inhibits thrombus. Amino acid zwitterion coating prevents protein deposition, alleviates the inflammatory process, inhibit of thrombosis, reduces the risk of implantable medical devices, and prolongs their service time. Hence, the work paves a new way to develop amino acid based zwitterionic polymer coating that can reduce the implant complications.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2400336"},"PeriodicalIF":4.4,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface Coating of ZIF-8 Nanoparticles with Polyacrylic Acid: A Facile Approach to Enhance Chemical Stability for Biomedical Applications. 用聚丙烯酸对 ZIF-8 纳米粒子进行表面涂层：增强生物医学应用中化学稳定性的简便方法。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-11-03 DOI: 10.1002/mabi.202400382

Setsuko Yamane, Abdul Hadi Bin Yusri, Po-Yu Chen, André J van der Vlies, Amira Ben Mabrouk, Isabelle Fetzer, Urara Hasegawa

{"title":"Surface Coating of ZIF-8 Nanoparticles with Polyacrylic Acid: A Facile Approach to Enhance Chemical Stability for Biomedical Applications.","authors":"Setsuko Yamane, Abdul Hadi Bin Yusri, Po-Yu Chen, André J van der Vlies, Amira Ben Mabrouk, Isabelle Fetzer, Urara Hasegawa","doi":"10.1002/mabi.202400382","DOIUrl":"https://doi.org/10.1002/mabi.202400382","url":null,"abstract":"Nanoparticles of zeolitic imidazole framework-8 (ZIF-8 NPs), which are the subclass of metal-organic frameworks consisting of Zn ion and 2-methylimidazole, have been identified as promising drug carriers since their large microporous structure is suited for encapsulating hydrophobic drug molecules. However, one of the limitations of ZIF-8 NPs is their low stability in physiological solutions, especially in the presence of water and phosphate anions. These molecules can interact with the coordinatively unsaturated Zn sites at the external surface to induce the degradation of ZIF-8 NPs. In this study, herein a facile approach is reported to enhance the chemical stability of ZIF-8 NPs by surface coating with polyacrylic acid (PAA). The PAA-coated ZIF-8 (PAA-ZIF-8) NPs are prepared by mixing ZIF-8 NPs and PAA in water. PAA coating inhibits the degradation of ZIF-8 NPs in water as well as phosphate-buffered saline over 6 days, which seems to be due to the coordination of carboxyl groups of PAA to the reactive Zn sites. Furthermore, the PAA-ZIF-8 NPs loaded with the anticancer drug doxorubicin (Dox) show cytotoxicity in human colon cancer cells. These results clearly show the feasibility of the PAA coating approach to improve the chemical stability of ZIF-8 NPs without impairing their drug delivery capability.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2400382"},"PeriodicalIF":4.4,"publicationDate":"2024-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0