3d打印壳聚糖-琼脂糖支架中氧化锌、氧化镁和氧化钙纳米粒子对抗菌和成骨效果的比较

IF 4.1 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Amir Hashemi, Masoumeh Ezati, Rima Paul, Inna Zumberg, Jaromir Bacovsky, Zdenka Fohlerova, Valentyna Provaznik
{"title":"3d打印壳聚糖-琼脂糖支架中氧化锌、氧化镁和氧化钙纳米粒子对抗菌和成骨效果的比较","authors":"Amir Hashemi, Masoumeh Ezati, Rima Paul, Inna Zumberg, Jaromir Bacovsky, Zdenka Fohlerova, Valentyna Provaznik","doi":"10.1002/mabi.202500232","DOIUrl":null,"url":null,"abstract":"<p><p>In the field of orthopedic surgery, large bone defects resulting from trauma, surgical resection, or congenital anomalies present significant challenges. In many cases, treatment necessitates scaffold structures that not only support bone regeneration but also address potential bacterial infections that can impede healing. In this study, we developed 3D bioprinted scaffolds using hydrogel-based biomaterial ink comprising a blend of chitosan (CS) and agarose (AG), each separately fortified with ZnO, MgO, and CaO nanoparticles (NPs). We performed a comprehensive assessment of the inks' printability and wettability, and ascertained their rheological properties. The in vitro degradation of 3D bioprinted scaffolds was analyzed, their antibacterial capabilities against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were explored, and the differentiation of bone marrow mesenchymal stem cells (BMSCs) was evaluated. The findings indicated that the hydrogel, CS-AG (CA), composed of 3.5% (w/v) CS and 1.5% (w/v) AG, demonstrated superior printing characteristics. Among the nanoparticles, ZnO proved to be a notable booster of antibacterial activity and facilitated osteogenic differentiation and proliferation of bone marrow stem cells. Conversely, MgO showed similar antibacterial efficacy but was less successful in promoting cell proliferation compared to ZnO and CaO, whereas CaO displayed the weakest antibacterial efficacy. The results identify the ZnO NP-loaded CA biomaterial ink as a viable option for addressing bone abnormalities, enhancing bone repair, and preventing bacterial infection.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00232"},"PeriodicalIF":4.1000,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparative Effects of ZnO, MgO, and CaO Nanoparticles in 3D-Printed Chitosan-Agarose Scaffolds on Antibacterial and Osteogenic Outcomes.\",\"authors\":\"Amir Hashemi, Masoumeh Ezati, Rima Paul, Inna Zumberg, Jaromir Bacovsky, Zdenka Fohlerova, Valentyna Provaznik\",\"doi\":\"10.1002/mabi.202500232\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>In the field of orthopedic surgery, large bone defects resulting from trauma, surgical resection, or congenital anomalies present significant challenges. In many cases, treatment necessitates scaffold structures that not only support bone regeneration but also address potential bacterial infections that can impede healing. In this study, we developed 3D bioprinted scaffolds using hydrogel-based biomaterial ink comprising a blend of chitosan (CS) and agarose (AG), each separately fortified with ZnO, MgO, and CaO nanoparticles (NPs). We performed a comprehensive assessment of the inks' printability and wettability, and ascertained their rheological properties. The in vitro degradation of 3D bioprinted scaffolds was analyzed, their antibacterial capabilities against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were explored, and the differentiation of bone marrow mesenchymal stem cells (BMSCs) was evaluated. The findings indicated that the hydrogel, CS-AG (CA), composed of 3.5% (w/v) CS and 1.5% (w/v) AG, demonstrated superior printing characteristics. Among the nanoparticles, ZnO proved to be a notable booster of antibacterial activity and facilitated osteogenic differentiation and proliferation of bone marrow stem cells. Conversely, MgO showed similar antibacterial efficacy but was less successful in promoting cell proliferation compared to ZnO and CaO, whereas CaO displayed the weakest antibacterial efficacy. The results identify the ZnO NP-loaded CA biomaterial ink as a viable option for addressing bone abnormalities, enhancing bone repair, and preventing bacterial infection.</p>\",\"PeriodicalId\":18103,\"journal\":{\"name\":\"Macromolecular bioscience\",\"volume\":\" \",\"pages\":\"e00232\"},\"PeriodicalIF\":4.1000,\"publicationDate\":\"2025-09-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Macromolecular bioscience\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1002/mabi.202500232\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Macromolecular bioscience","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1002/mabi.202500232","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

在骨科手术领域,由于创伤、手术切除或先天性异常导致的大面积骨缺损提出了重大挑战。在许多情况下,治疗需要支架结构,不仅支持骨再生,而且解决可能阻碍愈合的潜在细菌感染。在这项研究中,我们使用由壳聚糖(CS)和琼脂糖(AG)组成的水凝胶基生物材料墨水开发了3D生物打印支架,每种墨水分别添加ZnO, MgO和CaO纳米颗粒(NPs)。我们对油墨的印刷性和润湿性进行了全面的评估,并确定了它们的流变特性。分析生物3D打印支架的体外降解情况,探讨其对大肠杆菌(E. coli)和金黄色葡萄球菌(S. aureus)的抗菌能力,并评估其对骨髓间充质干细胞(BMSCs)的分化能力。结果表明,由3.5% (w/v) CS和1.5% (w/v) AG组成的水凝胶CS-AG (CA)具有优异的打印性能。在这些纳米颗粒中,氧化锌被证明是显著的抗菌活性增强剂,促进骨髓干细胞的成骨分化和增殖。相反,氧化镁的抑菌效果与氧化锌和氧化钙相似,但对细胞增殖的促进作用较弱,氧化钙的抑菌效果最弱。结果表明ZnO np负载的CA生物材料墨水是解决骨异常,增强骨修复和预防细菌感染的可行选择。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparative Effects of ZnO, MgO, and CaO Nanoparticles in 3D-Printed Chitosan-Agarose Scaffolds on Antibacterial and Osteogenic Outcomes.

In the field of orthopedic surgery, large bone defects resulting from trauma, surgical resection, or congenital anomalies present significant challenges. In many cases, treatment necessitates scaffold structures that not only support bone regeneration but also address potential bacterial infections that can impede healing. In this study, we developed 3D bioprinted scaffolds using hydrogel-based biomaterial ink comprising a blend of chitosan (CS) and agarose (AG), each separately fortified with ZnO, MgO, and CaO nanoparticles (NPs). We performed a comprehensive assessment of the inks' printability and wettability, and ascertained their rheological properties. The in vitro degradation of 3D bioprinted scaffolds was analyzed, their antibacterial capabilities against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were explored, and the differentiation of bone marrow mesenchymal stem cells (BMSCs) was evaluated. The findings indicated that the hydrogel, CS-AG (CA), composed of 3.5% (w/v) CS and 1.5% (w/v) AG, demonstrated superior printing characteristics. Among the nanoparticles, ZnO proved to be a notable booster of antibacterial activity and facilitated osteogenic differentiation and proliferation of bone marrow stem cells. Conversely, MgO showed similar antibacterial efficacy but was less successful in promoting cell proliferation compared to ZnO and CaO, whereas CaO displayed the weakest antibacterial efficacy. The results identify the ZnO NP-loaded CA biomaterial ink as a viable option for addressing bone abnormalities, enhancing bone repair, and preventing bacterial infection.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Macromolecular bioscience
Macromolecular bioscience 生物-材料科学:生物材料
CiteScore
7.90
自引率
2.20%
发文量
211
审稿时长
1.5 months
期刊介绍: Macromolecular Bioscience is a leading journal at the intersection of polymer and materials sciences with life science and medicine. With an Impact Factor of 2.895 (2018 Journal Impact Factor, Journal Citation Reports (Clarivate Analytics, 2019)), it is currently ranked among the top biomaterials and polymer journals. Macromolecular Bioscience offers an attractive mixture of high-quality Reviews, Feature Articles, Communications, and Full Papers. With average reviewing times below 30 days, publication times of 2.5 months and listing in all major indices, including Medline, Macromolecular Bioscience is the journal of choice for your best contributions at the intersection of polymer and life sciences.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信