Macromolecular bioscience最新文献_第2页

Continuous Chitosan/Poly (Vinyl Alcohol) Nanofiber in Collagen Hydrogel to Prepare Mechanically Robust Fibrous Nanocomposite for Tissue Engineering. 连续壳聚糖/聚乙烯醇纳米纤维在胶原水凝胶中制备组织工程用机械坚固的纳米纤维复合材料。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-07-07 DOI: 10.1002/mabi.202500230

Shakiba Kalhori, Ayoob Karimizade, Mohsen Sadeghi-Ghadikolaei, Masoud Siaghi, Amir Mellati, Somayeh Shahani

{"title":"Continuous Chitosan/Poly (Vinyl Alcohol) Nanofiber in Collagen Hydrogel to Prepare Mechanically Robust Fibrous Nanocomposite for Tissue Engineering.","authors":"Shakiba Kalhori, Ayoob Karimizade, Mohsen Sadeghi-Ghadikolaei, Masoud Siaghi, Amir Mellati, Somayeh Shahani","doi":"10.1002/mabi.202500230","DOIUrl":"https://doi.org/10.1002/mabi.202500230","url":null,"abstract":"Collagen (Col) hydrogel scaffolds require mechanical properties comparable to those of implanted tissues; however, their significant shrinkage, opacity, and rapid degradation hinder their application in tissue engineering and therapeutic contexts. Furthermore, polymer-reinforced Col hydrogels often lose their fibrous morphology, leading to a reduction in cell binding sites. In this study, we aim to enhance the mechanical properties and biodegradation resistance of Col hydrogels while preserving their fibrous microstructure. We achieve this by blending Col hydrogel with a continuous chitosan/poly(vinyl alcohol) nanofiber suspension (CS/PVA@NF), utilizing a wet electrospinning process coupled with a falling film collector. Morphological assessment of CS/PVA@NF reveals a well-defined nanofibrous microstructure, in contrast to the non-fibrous morphology observed in conventional polymer-blended hydrogels. The mechanical properties of the composite hydrogel improve up to 24-fold (Young's modulus: 120 kPa). The incorporation of CS/PVA@NF enhances cell attachment and proliferation potential. Subcutaneous implantation of the hydrogels in a murine model shows no notable inflammation. This research presents an effective method for improving Col hydrogels while maintaining their nanofibrous structure.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00230"},"PeriodicalIF":4.4,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144584292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering Efferocytosis for Bone Regeneration. 骨再生的工程Efferocytosis。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-07-04 DOI: 10.1002/mabi.202500094

Jacob Miszuk, Linna Zhong, Hongli Sun

{"title":"Engineering Efferocytosis for Bone Regeneration.","authors":"Jacob Miszuk, Linna Zhong, Hongli Sun","doi":"10.1002/mabi.202500094","DOIUrl":"10.1002/mabi.202500094","url":null,"abstract":"Bone is an incredibly robust tissue thanks to its high blood supply, rapid cell turnover, and continuous remodeling. A significant body of research investigates strategies to improve osteogenesis, angiogenesis, and immunomodulation for bone regeneration, facilitated by numerous various therapeutic approaches (e.g. pharmacologics, biomaterials, stem cell therapy, and more). However, a critically understudied but recently emerging area of research lies in the inflammatory cascade and the cleanup of apoptotic cells during repair, aging, and disease. Termed \"efferocytosis,\" this natural and efficient cleaning up of cells at the end of their lifespan is a crucial step in resolving injury, controlling disease, maintaining homeostasis, and tissue repair. Currently, the primary mechanism(s) driving efferocytosis in most tissue but especially bone, is unknown. Despite this knowledge gap, mounting evidence suggests that impaired efferocytosis plays a significant role in many chronic illnesses and impairs tissue regeneration. Biomaterials-based interventions are well-positioned to interrogate mechanisms of efferocytosis due to their ability to provide local support and guide cellular activity not only in combination with but also without additional pharmaceutical aid. This review will highlight the current understanding of efferocytosis in bone and discuss cutting-edge biomaterials-based strategies to engineer efferocytosis for improved outcomes in bone regeneration.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00094"},"PeriodicalIF":4.4,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12261927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144564871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanoparticle-Embedded GelMA/NIPAm Hydrogels: A Temperature-Responsive Hybrid System for Controlled Drug Release. 纳米颗粒嵌入凝胶/NIPAm水凝胶：用于控制药物释放的温度响应混合系统。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-06-29 DOI: 10.1002/mabi.202400635

Maria Daaboul, Ayse Akkaya, Zehra Kanli, Oguzhan Gunduz, Banu Aydın, Emine Alarcin, Mehmet Murat Ozmen, Murat Topuzogullari

{"title":"Nanoparticle-Embedded GelMA/NIPAm Hydrogels: A Temperature-Responsive Hybrid System for Controlled Drug Release.","authors":"Maria Daaboul, Ayse Akkaya, Zehra Kanli, Oguzhan Gunduz, Banu Aydın, Emine Alarcin, Mehmet Murat Ozmen, Murat Topuzogullari","doi":"10.1002/mabi.202400635","DOIUrl":"https://doi.org/10.1002/mabi.202400635","url":null,"abstract":"Temperature-responsive hydrogels incorporating drug-loaded polymeric nanoparticles represent a significant advancement in controlled release systems, enabling responsive and environmentally triggered drug delivery. In this study, a novel temperature-responsive drug delivery system was developed based on a gelatin methacryloyl/N-isopropylacrylamide (GelMA/NIPAm) hydrogel incorporating phenytoin (PHT)-loaded poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles. For this, empty nanoparticles, PHT-loaded nanoparticles, bare hydrogels (BH), empty nanoparticle-loaded hydrogels (eNP-H), and PHT-encapsulated nanoparticle-embedded hydrogels (PHT-H) were prepared and characterized using FTIR, SEM, DSC, XRD, DLS, swelling, drug release, and biocompatibility tests. The drug-loaded nanoparticles exhibited hydrodynamic diameter of 223.7 ± 8.4 nm with a PDI of 0.298 and a zeta potential of -20.4 mV. The BH, eNP-H, and PHT-H hydrogels displayed similar temperature-dependent swelling, with approximate weight swelling ratios of 9.0 at 25°C, 7.5 at 37°C, and 6.0 at 40°C. Swelling kinetics showed that all hydrogels reached equilibrium within 20 min. Moreover, the hydrogels demonstrated consistent cyclic swelling and shrinking at 37°C and 40°C. Drug release studies revealed that PHT-H hydrogels released ∼20% of phenytoin at 37°C and ∼34% at 40°C over 7 days, confirming sustained, temperature-responsive drug release. Cell viability assays indicated no cytotoxicity and potential promotion of cell proliferation. Thus, these hydrogels offer a promising platform for efficient, temperature-sensitive, and controlled drug delivery applications.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00635"},"PeriodicalIF":4.4,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amphiphilic Poly(γ-glutamic acid) Derivatives for Delivering Doxorubicin in Cancer Cells. 两亲性聚γ-谷氨酸衍生物在癌细胞中递送阿霉素。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-06-22 DOI: 10.1002/mabi.202500090

Porochista Dorost, Miguel R G Morais, Sofia Cunha Guimarães, Ana Paula Pêgo, Montserrat García-Alvarez, Antxon Martínez de Ilarduya

{"title":"Amphiphilic Poly(γ-glutamic acid) Derivatives for Delivering Doxorubicin in Cancer Cells.","authors":"Porochista Dorost, Miguel R G Morais, Sofia Cunha Guimarães, Ana Paula Pêgo, Montserrat García-Alvarez, Antxon Martínez de Ilarduya","doi":"10.1002/mabi.202500090","DOIUrl":"https://doi.org/10.1002/mabi.202500090","url":null,"abstract":"One of the most limiting factors in cancer treatment is the difficulty of delivering anticancer agents effectively to tumor sites. To overcome this challenge, this research focuses on developing a carrier that has been modified to possess amphiphilic properties while remaining biodegradable and biocompatible. Amphiphilic esters derived from poly(γ-glutamic acid) by modifying the carboxylate side groups with 4-phenyl-butyl bromide were prepared. These copolymers self-assembled into nanoparticles via nanoprecipitation. The cytocompatibility of the nanoparticles was assessed through lactate dehydrogenase release and metabolic activity of U-87 glioma cells. Fluorescein isothiocyanate labeling demonstrated effective cellular uptake of nanoparticles. These nanoparticles were further decorated with polyethylene glycol (PEG) and a PEG-folic acid conjugate (FA-PEG-NH2), their sizes being 174 and 156 nm, respectively. Successful grafting was confirmed through 1H-NMR and FTIR spectroscopy. The nanoparticles were loaded with doxorubicin, and release studies showed their sensitivity to the pH of the environment, the encapsulated drug being released faster at pH 4.2 compared to pH 7.4. Encapsulated doxorubicin's effect on U-87 cells was tested at various concentrations and time points, showing significantly better performance compared to free doxorubicin. These results suggest that those poly(γ-glutamic acid) derivatives hold great promise for improving the delivery of hydrophobic drugs and enhancing cancer treatment.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00090"},"PeriodicalIF":4.4,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Front Cover: Macromol. Biosci. 6/2025

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-06-16 DOI: 10.1002/mabi.202570011

引用次数: 0

Issue Information: Macromol. Biosci. 6/2025 资料:宏mol。Biosci 6/2025。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-06-16 DOI: 10.1002/mabi.202570012

引用次数: 0

P65-Driven MIR4435-2HG Enhances Prognostic Value and Mediates Oxaliplatin Resistance via the miR-378G/ABCB9 Axis in Colorectal Cancer. p65驱动的MIR4435-2HG通过miR-378G/ABCB9轴增强结直肠癌预后价值并介导奥沙利铂耐药

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-06-10 DOI: 10.1002/mabi.202400663

Xiaopeng Zhuang, Jinji Jin, Jun Cheng, Zhejing Chen, Weijian Zhu, Xielin Huang, Jiancheng Sun, Chengyin Lin, Qiantong Dong, Dongwang Yan, Xiaoliang Qi, Wenyi Wu

{"title":"P65-Driven MIR4435-2HG Enhances Prognostic Value and Mediates Oxaliplatin Resistance via the miR-378G/ABCB9 Axis in Colorectal Cancer.","authors":"Xiaopeng Zhuang, Jinji Jin, Jun Cheng, Zhejing Chen, Weijian Zhu, Xielin Huang, Jiancheng Sun, Chengyin Lin, Qiantong Dong, Dongwang Yan, Xiaoliang Qi, Wenyi Wu","doi":"10.1002/mabi.202400663","DOIUrl":"https://doi.org/10.1002/mabi.202400663","url":null,"abstract":"Long non-coding RNA MIR4435-2HG has emerged as a pivotal oncogenic factor across various cancers. However, its role in chemoresistance, particularly in colorectal cancer (CRC), remains unclear. This work demonstrates that MIR4435-2HG is significantly overexpressed in CRC tissues, correlating with poor prognosis and resistance to oxaliplatin (L-OHP) based chemotherapy. Mechanistically, MIR4435-2HG binds to miR-378g, leading to elevated ABCB9 levels, a crucial factor in drug resistance. Both in vitro and in vivo experiments indicate that the MIR4435-2HG/miR-378g/ABCB9 axis confers L-OHP resistance in CRC cells by reducing DNA damage and enhancing cell survival. Additionally, P65, a component of the NF-κB pathway, directly promotes MIR4435-2HG transcription, triggering subsequent chemoresistance. Based on these results, MIR4435-2HG is recognized as a reliable prognostic marker and serves as a target for therapeutic strategies, presenting new approaches to counteract L-OHP resistance and enhance CRC patient outcomes.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00663"},"PeriodicalIF":4.4,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144258322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnetically Actuated Cell Culture Platform for Controlling High-Throughput Cyclic Strain. 控制高通量循环菌株的磁驱动细胞培养平台。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-06-10 DOI: 10.1002/mabi.202400466

Bryce J Stottlemire, Aparna R Chakravarti, Cory J Berkland

{"title":"Magnetically Actuated Cell Culture Platform for Controlling High-Throughput Cyclic Strain.","authors":"Bryce J Stottlemire, Aparna R Chakravarti, Cory J Berkland","doi":"10.1002/mabi.202400466","DOIUrl":"https://doi.org/10.1002/mabi.202400466","url":null,"abstract":"Cells efficiently manage various mechanical inputs, converting them into biochemical outputs to regulate function. Mechanobiologists aim to harness this capacity by developing platforms that mimic physiological mechanical environments. Current commercial and research-based dynamic cell culture platforms rely on external force generators to control substrate deformation or translation. However, this tends to make the systems bulky, and can sacrifice throughput and adaptability. Thus, this work presents the advancement of magnetic-polydimethylsiloxane (PDMS) cell culture systems to precisely control the mechanical strain environment of 2D and 3D cell cultures with multiple high-throughput embodiments. First, an indirect 3D fabrication technique is utilized to develop high-fidelity, deformable microporous magnetic composite material for high-throughput cyclic straining of a 3D hydrogel. Second, a magnetic PDMS membrane is developed for 2D cell culture to mimic the complex and nonhomogeneous mechanical environment cells experience in vivo. The proposed advancements can significantly shift cell culture technologies by leveraging magnetic responsive materials to develop dynamic bioreactor systems with diverse interfaces and high throughput capabilities, enabling precise control over cellular environments with diverse strain profiles and gradients for more sophisticated cell behavior and differentiation studies.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00466"},"PeriodicalIF":4.4,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144258321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to "Biocompatible Composite Microspheres of Chitin/Ordered Mesoporous Carbon CMK3 for Bilirubin Adsorption and Cell Microcarrier Culture". 对“几丁质/有序介孔碳CMK3生物相容性复合微球吸附胆红素及细胞微载体培养”的修正。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-06-02 DOI: 10.1002/mabi.202500264

引用次数: 0

Photocurable and 3D Printable Functional Polyesters to Engineer Elastomeric Scaffolds for Biomedical Applications. 用于生物医学应用的工程弹性支架的光固化和3D打印功能聚酯。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-05-30 DOI: 10.1002/mabi.202500189

Xiaochu Ding, Narangerel Gantumur, Bruce P Lee, Ying Grace Chen

{"title":"Photocurable and 3D Printable Functional Polyesters to Engineer Elastomeric Scaffolds for Biomedical Applications.","authors":"Xiaochu Ding, Narangerel Gantumur, Bruce P Lee, Ying Grace Chen","doi":"10.1002/mabi.202500189","DOIUrl":"https://doi.org/10.1002/mabi.202500189","url":null,"abstract":"Photocurable functional block copolyesters are reported to engineer elastomeric scaffolds for biomedical applications. The polymer backbone is organized by soft and stiff blocks. The functional prepolymer is readily crosslinked by thiol-yne click chemistry under ulraviolet light in the presence of a photo-initiator to form a robust elastomer. The elastomers bear both chemical crosslinks and crystal-domain crosslinks to simultaneously tune the materials' properties, such as mechanical properties and degradation rates. The dual crosslinks can more efficiently tune the mechanical properties compared to the chemical crosslink alone. More importantly, the functional prepolymer is photo-printable to construct elastomeric scaffolds with precise control of pore sizes using the state-of-the-art digital light processing technique. With hydroxyls pendant on the backbone, human umbilical vein endothelial cells prefer to grow on the elastomer surface compared to that of a poly(caprolactone) film. It is believed that these functional photo-polyesters will be useful to construct medical devices for bioengineering research.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00189"},"PeriodicalIF":4.4,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0