Macromolecular bioscience最新文献_第2页

Preclinical Testing of 3D Printed, Cell Loaded Hydrogel Based Corneal Substitutes on Rabbit Model. 3D打印细胞负载水凝胶角膜代用品在兔模型上的临床前测试

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-03-27 DOI: 10.1002/mabi.202400595

Deniz Basoz, Aslihan Akalinli, Senem Buyuksungur, A R Cenk Celebi, Deniz Yucel, Nesrin Hasirci, Vasif Hasirci

{"title":"Preclinical Testing of 3D Printed, Cell Loaded Hydrogel Based Corneal Substitutes on Rabbit Model.","authors":"Deniz Basoz, Aslihan Akalinli, Senem Buyuksungur, A R Cenk Celebi, Deniz Yucel, Nesrin Hasirci, Vasif Hasirci","doi":"10.1002/mabi.202400595","DOIUrl":"https://doi.org/10.1002/mabi.202400595","url":null,"abstract":"Many people lose their vision due to corneal stroma injuries of the eye and the golden solution is transplantation of allografts from donors. Unfortunately, the limited availability of donor tissue, risk of disease transmission, and immune rejection are serious handicaps. However, implants made of biomaterials can be used as substitutes. In this study, cell-loaded and cell-free, methacrylated gelatin (GelMA) implants are 3D printed and tested under in vitro conditions. The samples are physically characterized for their printability, equilibrium water content, compressive mechanical strength, and transparency; they retained 60%-80% of light transmission in the visible region as in the native corneas. In brief, they are suitable for further testing. Then cell loaded samples are tested in vivo on New Zealand white rabbits for 90 days. In the in vivo tests, these cell loaded, disk shaped implants are almost completely degraded and allowed reorganization of the tissue forming at the implantation site. Also, the immune response initially observed decreased in time and by the end of 90 days the tissue regained its normal, healthy architecture with multilayered, non-keratinized epithelium. It can be concluded that the implants developed in this study are promising for clinical use in corneal stroma recovery.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2400595"},"PeriodicalIF":4.4,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Polymer-Based Piezoelectric Materials for the Bone Tissue Regeneration. 用于骨组织再生的聚合物基压电材料的研制。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-03-25 DOI: 10.1002/mabi.202500031

Madappa C Maridevaru, Honglang Lu, Shubham Roy, Yuqian Yan, Fei Wang, Sai Ko Soe, Zia Ullah, Hongxun Sang, Jian Shang, Bing Guo

{"title":"Development of Polymer-Based Piezoelectric Materials for the Bone Tissue Regeneration.","authors":"Madappa C Maridevaru, Honglang Lu, Shubham Roy, Yuqian Yan, Fei Wang, Sai Ko Soe, Zia Ullah, Hongxun Sang, Jian Shang, Bing Guo","doi":"10.1002/mabi.202500031","DOIUrl":"https://doi.org/10.1002/mabi.202500031","url":null,"abstract":"With the aging population, fitness issues, poor bone healing, and high infection rates are associated with bone fractures and other bone diseases. Nevertheless, traditional approaches and materials struggle to treat orthopedic diseases by loading exogenous stem cells, growth factors, or merely structurally simulating the bone periosteum. The advancement of biomedical materials has become critical in addressing the challenges associated with bone tissue regeneration, encompassing a range of conditions including bone wounds, inflammation, infections, fractures, and the degenerative effects of aging or metabolic disorders. Recently, polymer-based piezoelectric materials have emerged as a promising avenue for enhancing regenerative processes. These materials possess unique electrical properties that can stimulate cellular activities and promote healing, making them particularly suitable for bone tissue engineering applications. This review aims to delineate the multifaceted role of polymer-based piezoelectric materials in treating various bone-related ailments, highlighting their potential for active regeneration actively and pointing out the challenges and future research directions for piezoelectric materials in regenerative medicine for bone tissue engineering. By integrating insights from materials science and biomedical engineering to develop more effective strategies for managing bone tissue injuries and disorders, ultimately enhancing bone healing, promoting osseointegration, and accelerating tissue regeneration.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2500031"},"PeriodicalIF":4.4,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnetic Levitational Assembly of Differentiated SH-SY5Y Cells for Aβ-Induced 3D Alzheimer's Disease Modeling and Curcumin Screening. a β诱导阿尔茨海默病三维建模及姜黄素筛选的SH-SY5Y分化细胞磁悬浮组装

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-03-25 DOI: 10.1002/mabi.202400658

Rumeysa Bilginer-Kartal, Ahu Arslan-Yildiz

{"title":"Magnetic Levitational Assembly of Differentiated SH-SY5Y Cells for Aβ-Induced 3D Alzheimer's Disease Modeling and Curcumin Screening.","authors":"Rumeysa Bilginer-Kartal, Ahu Arslan-Yildiz","doi":"10.1002/mabi.202400658","DOIUrl":"https://doi.org/10.1002/mabi.202400658","url":null,"abstract":"Alzheimer's disease is one of the prevalent neurodegenerative diseases and is characterized by amyloid beta aggregate (Aβ) accumulation. This study reports an Aβ 1-42 induced 3D Alzheimer's disease modeling utilizing differentiated SH-SY5Y spheroids, which is carried out by Magnetic levitation approach, and the neuroprotective effect of Curcumin is further investigated on this model. For this purpose, SH-SY5Y spheroids are differentiated using Retinoic acid-Brain-derived neurotrophic factor sequentially during 3D cell culture. Differentiated spheroids maintained high viability and exhibited significant neuronal characteristics, as evidenced by increasing β-III tubulin and NeuN expressions. 3D Alzheimer's disease model formation and neurotoxicity of Aβ 1-42 aggregates are investigated on un-/differentiated spheroids, resulting in 65% and 51% cell viability, respectively. Characterization of the 3D Alzheimer's disease model is done by immunostaining of Choline acetyltransferase to investigate cholinergic neuron activity loss, showing a 2.2 decrease in fluorescence intensity. Further, Curcumin treatment on the 3D Alzheimer's disease model resulted in augmenting cell viability, confirming neuroprotective effect of Curcumin on Aβ 1-42 induced Alzheimer's disease model. This study highlighted the magnetic levitation-based fabrication of Aβ 1-42-induced 3D Alzheimer's disease model successfully, offering a promising experimental platform for other neurodegenerative disease research and potential clinical applications.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2400658"},"PeriodicalIF":4.4,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mild Oxidation of Plant Polyphenols Yields Modular Nanoparticle-Forming Materials with Drug Loading Capabilities. 植物多酚的轻度氧化产生具有药物装载能力的模块化纳米颗粒形成材料。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-03-25 DOI: 10.1002/mabi.202400529

Suhair Sunoqrot, Samah Abusulieh, Ilya Anufriev, Philipp Dahlke, Paul M Jordan, Eveen Al-Shalabi, Caroline T Holick, Stephanie Hoeppener, Ivo Nischang, Oliver Werz, Stephanie Schubert, Ulrich S Schubert

{"title":"Mild Oxidation of Plant Polyphenols Yields Modular Nanoparticle-Forming Materials with Drug Loading Capabilities.","authors":"Suhair Sunoqrot, Samah Abusulieh, Ilya Anufriev, Philipp Dahlke, Paul M Jordan, Eveen Al-Shalabi, Caroline T Holick, Stephanie Hoeppener, Ivo Nischang, Oliver Werz, Stephanie Schubert, Ulrich S Schubert","doi":"10.1002/mabi.202400529","DOIUrl":"https://doi.org/10.1002/mabi.202400529","url":null,"abstract":"Plant polyphenols have attracted interest as green precursors for functional materials due to their unique chemical features. Here, an approach to formulate nanoparticles (NPs) from a hydrophobic (quercetin; QCT) and a hydrophilic (tannic acid; TA) polyphenol, by leveraging their propensity for oxidation, is presented. QCT and TA are partially oxidized to form oxQCT and oxTA, respectively. The materials are subsequently used to formulate NPs by nanoprecipitation alone or in the presence of hydrophilic and amphiphilic polymers. Characterization of oxQCT reveals a notable chemical change and increased thermal stability, with reduced antioxidant and anti-inflammatory activities compared to unmodified QCT. Conversely, oxTA shows an insignificant change in chemistry compared to pristine TA, yet it displays greater thermal stability, reduced antioxidant activity, and altered anti-inflammatory activity. Particle size and morphology of the formulated NPs are examined by dynamic light scattering (DLS), analytical ultracentrifugation (AUC), and transmission electron microscopy (TEM). The results indicate that co-formulating oxQCT and oxTA with different polymers impacts their particle size, polydispersity index, and morphology. Lastly, oxQCT and oxTA co-formulated with other polymers are capable of loading hydrophobic drugs such as amphotericin B and curcumin, forming sub-200 nm NPs with high loading efficiencies, which validates their use in drug delivery systems.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2400529"},"PeriodicalIF":4.4,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Supramolecular, Triple Negative Breast Cancer-Targeting Avidin-Photosensitizer. 一种超分子的三阴性乳腺癌靶向亲和素光敏剂。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-03-25 DOI: 10.1002/mabi.202400610

Bingjie Gao, Charlotte Schäfers, Seah Ling Kuan, Tanja Weil

{"title":"A Supramolecular, Triple Negative Breast Cancer-Targeting Avidin-Photosensitizer.","authors":"Bingjie Gao, Charlotte Schäfers, Seah Ling Kuan, Tanja Weil","doi":"10.1002/mabi.202400610","DOIUrl":"https://doi.org/10.1002/mabi.202400610","url":null,"abstract":"The potential of photodynamic therapy (PDT) in combination with chemotherapy to improve treatment outcomes for triple-negative breast cancer (TNBC), for which no targeted therapy is available, is the subject of considerable investigation. In PDT, photosensitizers (PSs) are frequently administered directly but do not selectively target cancer cells. To address the delivery of a PS to TNBC and enhance cellular uptake, the Ru-NH2-modified avidin bioconjugate (RuAvi) via Tyr-specific modification using the Mannich reaction is prepared. The RuAvi is further assembled with the cinnamoyl peptide-F(D)LF(D)LFK-NH2 (FK), which binds to formyl peptide receptor 1, overexpressed in TNBC. Notably, the modified Avi still possesses the ability to efficiently bind biotin for the assembly of up to four copies of the FK peptides. The resultant FK4-RuAvi exhibited an IC50 value of 0.36 ± 0.08 µM, which is ≈3.5-fold lower than that of RuAvi (1.25 ± 0.09 µM), upon irradiation in the triple-negative MDA-MB-231 breast cancer cells. FK4-RuAvi also shows efficient uptake in MDA-MB-231 tumor spheroids and exhibited significant toxicity after irradiation compared to the control RuAvi. The presented strategy has the potential to improve the efficacy of targeted PDT to meet the high demand for targeted therapies to treat TNBC, such as targeted adjuvant treatment after breast cancer surgery.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2400610"},"PeriodicalIF":4.4,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioprinting of GelMA/PEGDA Hybrid Bioinks for SH-SY5Y Cell Encapsulation: Role of Molecular Weight and Concentration. 凝胶/聚乙二醇杂化生物墨水在SH-SY5Y细胞包封中的生物打印：分子量和浓度的作用。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-03-25 DOI: 10.1002/mabi.202400587

Hexin Yue, Yaxin Wang, Samantha Fernandes, Cian Vyas, Paulo Bartolo

{"title":"Bioprinting of GelMA/PEGDA Hybrid Bioinks for SH-SY5Y Cell Encapsulation: Role of Molecular Weight and Concentration.","authors":"Hexin Yue, Yaxin Wang, Samantha Fernandes, Cian Vyas, Paulo Bartolo","doi":"10.1002/mabi.202400587","DOIUrl":"https://doi.org/10.1002/mabi.202400587","url":null,"abstract":"Current clinical interventions for large peripheral nerve gap injuries are limited. Bioprinting provides opportunities to develop tissue engineered constructs that provide a biomimetic environment to guide nerve regeneration. However, hydrogels that are cell-instructive, mechanically compliant, and have an appropriate biodegradation profile for nerve guidance conduit applications are limited. In this study, a photocrosslinkable gelatin methacryloyl (GelMA) and polyethylene glycol diacrylate (PEGDA) hybrid bioink is developed. The role of PEGDA molecular weight and concentration in tuning the hydrogel physicochemical and biological properties is evaluated. PEGDA modulated the hydrogel network structure and properties in a molecular weight and concentration dependent manner. A lower molecular weight and high concentration induced high crosslinking density thus improving compressive modulus, lower swelling, and a slower degradation profile. The bioinks showed good printability and are able to fabricate multi-layer constructs with high shape fidelity and flexibility. The SH-SY5Y cells maintained high cell viability after bioprinting in all bioinks. However, cells showed limited metabolic activity and spreading in the GelMA/PEGDA hydrogels with both high concentration and molecular weight. This preliminary study provides guidance on the use of specific molecular weights and concentrations in GelMA/PEGDA bioinks for the bioprinting of SH-SY5Y cells.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2400587"},"PeriodicalIF":4.4,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancements in Artificial Cell Preparation and Biomedical Applications. 人工细胞制备及其生物医学应用进展。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-03-25 DOI: 10.1002/mabi.202500052

Jiayan Liu, Yong Pan, Zhi Liu, Yanxin Qi, Yubin Huang

{"title":"Advancements in Artificial Cell Preparation and Biomedical Applications.","authors":"Jiayan Liu, Yong Pan, Zhi Liu, Yanxin Qi, Yubin Huang","doi":"10.1002/mabi.202500052","DOIUrl":"https://doi.org/10.1002/mabi.202500052","url":null,"abstract":"Artificial cells represent a transformative innovation in biomedical science, offering engineered, cell-like constructs capable of mimicking certain biological functions. Although remarkable progress has been made to construct complex multifunctional artificial cells, there are still significant differences between artificial cells and natural cells. Thus, a thorough understanding of the techniques and challenges associated with the fabrication of artificial cells, alongside their applications, is essential for driving further technological progress in this field. This review aims to summarize recent advances in the preparation techniques and biomedical applications of artificial cells. In biomedical applications, emphasize their potential to revolutionize cancer therapeutics, gene therapy, immunotherapy, vaccine, reactors for the manufacture of biomolecules and signal communication. Artificial cells are primarily fabricated through bottom-up approaches, including liposomes, metal-organic frameworks, colloidal, hydrogel, and droplet microfluidic which allow for precise control over their composition, structure, and functionality. Finally, the future outlook and challenges for the development of artificial cells simulating the real activities of natural cells are described.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2500052"},"PeriodicalIF":4.4,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodegradable Polymers for Plant Nutrient Delivery and Recovery. 用于植物养分输送和恢复的可生物降解聚合物。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-03-25 DOI: 10.1002/mabi.202500042

Alice Boarino, Nicola Carrara, Elio Padoan, Luisella Celi, Harm-Anton Klok

{"title":"Biodegradable Polymers for Plant Nutrient Delivery and Recovery.","authors":"Alice Boarino, Nicola Carrara, Elio Padoan, Luisella Celi, Harm-Anton Klok","doi":"10.1002/mabi.202500042","DOIUrl":"https://doi.org/10.1002/mabi.202500042","url":null,"abstract":"The current use of fertilizers is inefficient and not sustainable. The majority of the fertilizer applied does not reach the targeted crop but is lost in the water bodies and into the atmosphere, with harmful impact on the environment. To enhance the efficiency and sustainability of current agricultural practices, it is essential to address two complementary challenges. First, nutrient delivery methods must be refined to maximize plant uptake. Second, the recovery of nutrients from wastewater and other waste streams should be improved to enhance the recycling of nitrogen and phosphorous and reduce environmental pollution. Biodegradable polymers hold great promise for the development of technological solutions toward more sustainable agricultural practices. This review covers the application of biodegradable polymers in both aspects of the nutrient cycle: nutrient delivery to plants through slow- and controlled-release fertilizers, and nutrient recovery from wastewater using membrane separation, adsorbent composites, and coagulants/flocculants. The most promising materials are highlighted for both approaches, identifying the research gaps and discussing potential future directions in this highly significant field.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2500042"},"PeriodicalIF":4.4,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrospun Fiber Membranes Modified with Heparin and Parathyroid Hormone Derivative To Promote Diabetic Wound Healing. 肝素和甲状旁腺激素衍生物修饰的电纺丝纤维膜促进糖尿病伤口愈合。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-03-25 DOI: 10.1002/mabi.202400608

Xinxin Wu, Zhuowen Hao, Zepu Wang, Jizhao Dong, Mengyue Zhu, Renxin Chen, Zouwei Li, Junwu Wang, Guang Shi, Tonghe Zhu, Jingfeng Li

{"title":"Electrospun Fiber Membranes Modified with Heparin and Parathyroid Hormone Derivative To Promote Diabetic Wound Healing.","authors":"Xinxin Wu, Zhuowen Hao, Zepu Wang, Jizhao Dong, Mengyue Zhu, Renxin Chen, Zouwei Li, Junwu Wang, Guang Shi, Tonghe Zhu, Jingfeng Li","doi":"10.1002/mabi.202400608","DOIUrl":"https://doi.org/10.1002/mabi.202400608","url":null,"abstract":"The repair of diabetic wounds is a global challenge due to elevated levels of blood sugar and microvascular disorders. Parathyroid hormone (PTH) and its derivatives show great potential for wound healing, but corresponding delivery strategies are still limited. Herein, a novel electrospun fiber membrane whose surface is modified with heparin is fabricated to deliver a PTH derivative named parathyroid hormone related peptide-1 (PTHrP-1) for the healing of diabetic wounds. Cell viability, proliferation, migration, and proangiogenic activity are first evaluated in vitro, and then a rat diabetic skin defect model is used to observe the in vivo effects on wound healing. Results of in vitro and in vivo studies confirm that electrospun fiber membrane modified with heparin and PTHrP-1 promotes the proliferation and migration of endothelial cells and fibroblasts, and facilitates diabetic wound healing through angiogenesis, collagen deposition, and re-epithelialization. In conclusion, electrospun fiber membrane modified with heparin shows great promise in delivering PTHrP-1 and promoting diabetic wound healing.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2400608"},"PeriodicalIF":4.4,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Viscoelasticity Can Be Tuned Through Covalent Incorporation of Chondroitin Sulphate in Allylated Gelatin Hydrogels. 粘弹性可以通过在烯丙化明胶水凝胶中加入硫酸软骨素共价来调节。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-03-19 DOI: 10.1002/mabi.202400422

Manuela A Boos, Khoon S Lim, Shireen R Lamandé, Kathryn S Stok

{"title":"Viscoelasticity Can Be Tuned Through Covalent Incorporation of Chondroitin Sulphate in Allylated Gelatin Hydrogels.","authors":"Manuela A Boos, Khoon S Lim, Shireen R Lamandé, Kathryn S Stok","doi":"10.1002/mabi.202400422","DOIUrl":"https://doi.org/10.1002/mabi.202400422","url":null,"abstract":"Cartilage is a slow-remodeling tissue with limited healing capacity. This has led to decades of tissue engineering efforts where the goal is biomaterials with regenerative capacity to restore functional integrity. Achieving full functional and mechanical integrity has proven difficult as cartilage has distinct mechanical properties. Glycosaminoglycans (GAGs) play a crucial role in cartilage mechanics due to their swelling behavior, contributing to viscoelasticity. The aims of this study are to covalently incorporate thiolated chondroitin sulphate (CSSH) in allylated gelatin (gelAGE) hydrogels at different concentrations to mimic GAG-rich regions in cartilage and create platforms to study subsequent cellular behavior. Hydrogels are evaluated for soluble fraction, swelling ratio, chondroitin sulphate (CS) retention, mechanical and viscoelastic properties, and cytocompatibility. ≈80% of CSSH is retained, and samples containing CSSH has an increased swelling ratio, indicating the incorporation of GAGs. Samples containing CSSH has an increased relaxation amplitude compared to gelAGE controls with a more elastic response. The addition of CSSH has no adverse effects on cytocompatibility. In conclusion, this study demonstrates the incorporation of thiolated CS in gelAGE hydrogels at different concentrations with no adverse effects on cytocompatibility. This allows for viscoelastic tuning which is important to consider when engineering new biomaterials.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2400422"},"PeriodicalIF":4.4,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0