Macromolecular bioscience最新文献_第2页

BMP2 Binds Non-Specifically to PEG-Passivated Biomaterials and Induces pSMAD 1/5/9 Signalling. BMP2 与 PEG-钝化生物材料非特异性结合并诱导 pSMAD 1/5/9 信号传导

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-08-31 DOI: 10.1002/mabi.202400169

Jean Le Pennec, Amaury Guibert, Raviteja Gurram, Antoine Delon, Romain R Vivès, Elisa Migliorini

{"title":"BMP2 Binds Non-Specifically to PEG-Passivated Biomaterials and Induces pSMAD 1/5/9 Signalling.","authors":"Jean Le Pennec, Amaury Guibert, Raviteja Gurram, Antoine Delon, Romain R Vivès, Elisa Migliorini","doi":"10.1002/mabi.202400169","DOIUrl":"https://doi.org/10.1002/mabi.202400169","url":null,"abstract":"Biomaterials are widely employed across diverse biomedical applications and represent an attractive strategy to explore how extracellular matrix components influence cellular response. In this study, the previously developed streptavidin platforms is aimed to use to investigate the role of glycosaminoglycans (GAGs) in bone morphogenetic protein 2 (BMP2) signaling. However, it is observed that the interpretation of findings is skewed due to the GAG-unrelated, non-specific binding of BMP2 on components of biomaterials. Non-specific adsorption of proteins is a recurrent and challenging issue for biomaterial studies. Despite the initial incorporation of anti-fouling polyethylene glycol (PEG) chains within biomaterials, the residual non-specific BMP2 adsorption still triggered BMP2 signaling within the same range as conditions of interest. The various options are explored to prevent BMP2 non-specific adsorption and a successful blocking condition involving a combination of bovine serum albumin and trehalose are identified. Furthermore, the effect of this blocking step improved when using gold platforms instead of glass, particularly with Chinese hamster ovary (CHO) cells. With this specific example, it is suggested that non-specific adsorption of BMPs on biomaterials may be a general concern - often undetected by classical surface-sensitive techniques - that needs to be addressed to better interpret cellular responses.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142108934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glucose Oxidase-Coated Calcium Peroxide Nanoparticles as an Innovative Catalyst for In Situ H₂O₂-Releasing Hydrogels. 葡萄糖氧化酶包覆过氧化钙纳米粒子作为原位 H2O2 释放水凝胶的创新催化剂。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-08-29 DOI: 10.1002/mabi.202400268

Phuong Le Thi, Quang Anh Tu, Dong Hwan Oh, Ki Dong Park

{"title":"Glucose Oxidase-Coated Calcium Peroxide Nanoparticles as an Innovative Catalyst for In Situ H2O2-Releasing Hydrogels.","authors":"Phuong Le Thi, Quang Anh Tu, Dong Hwan Oh, Ki Dong Park","doi":"10.1002/mabi.202400268","DOIUrl":"https://doi.org/10.1002/mabi.202400268","url":null,"abstract":"In situ forming and hydrogen peroxide (H2O2)-releasing hydrogels have been considered as attractive matrices for various biomedical applications. Particularly, horseradish peroxidase (HRP)-catalyzed crosslinking reaction serves efficient method to create in situ forming hydrogels due to its advantageous features, such as mild reaction conditions, rapid gelation rate, tunable mechanical strength, and excellent biocompatibility. Herein, a novel HRP-crosslinked hydrogel system is reported that can produce H2O2 in situ for long-term applications, using glucose oxidase-coated calcium peroxide nanoparticles (CaO2@GOx NPs). In this system, CaO2 gradually produced H2O2 to support the HRP-mediated hydrogelation, while GOx further catalyzed the oxidation of glucose for in situ H2O2 generation. As the hydrogel is formed rapidly is expected and the H2O2 release behavior is prolonged up to 10 days. Interestingly, hydrogels formed by HRP/CaO2@GOx-mediated crosslinking reaction provided a favorable 3D microenvironment to support the viability and proliferation of fibroblasts, compared to that of hydrogels formed by either HRP/H2O2 or HRP/CaO2/GOx-mediated crosslinking reaction. Furthermore, HRP/CaO2@GOx-crosslinked hydrogel enhanced the angiogenic activities of endothelial cells, which is demonstrated by the in vitro tube formation test and in ovo chicken chorioallantoic membrane model. Therefore, HRP/CaO2@GOx-catalyzed hydrogels is suggested as potential in situ H2O2-releasing materials for a wide range of biomedical applications.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142108935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Synergistic Potential of Hydrogel Microneedles and Nanomaterials: Breaking Barriers in Transdermal Therapy. 水凝胶微针与纳米材料的协同潜力：打破透皮疗法的障碍。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-08-28 DOI: 10.1002/mabi.202400228

Atefeh Golshirazi, Mahsa Mohammadzadeh, Sheyda Labbaf

{"title":"The Synergistic Potential of Hydrogel Microneedles and Nanomaterials: Breaking Barriers in Transdermal Therapy.","authors":"Atefeh Golshirazi, Mahsa Mohammadzadeh, Sheyda Labbaf","doi":"10.1002/mabi.202400228","DOIUrl":"https://doi.org/10.1002/mabi.202400228","url":null,"abstract":"The stratum corneum, which acts as a strong barrier against external agents, presents a significant challenge to transdermal drug delivery. In this regard, microneedle (MN) patches, designed as modern systems for drug delivery via permeation through the skin with the ability to pass through the stratum corneum, are known to be convenient, painless, and effective. In fact, MN have shown significant breakthroughs in transdermal drug delivery, and among the various types, hydrogel MN (HMNs) have demonstrated desirable inherent properties. Despite advancements, issues such as limited loading capacity, uncontrolled drug release rates, and non-uniform therapeutic approaches persist. Conversely, nanomaterials (NMs) have shown significant promise in medical applications, however, their efficacy and applicability are constrained by challenges including poor stability, low bioavailability, limited payload capacity, and rapid clearance by the immune system. Incorporation of NMs within HMNs offers new prospects to address the challenges associated with HMNs and NMs. This combination can provide a promising field of research for improved and effective delivery of therapeutic agents and mitigate certain adverse effects, addressing current clinical concerns. The current review highlights the use of NMs in HMNs for various therapeutic and diagnostic applications.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142080797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Substrates with Tunable Hydrophobicity for Optimal Cell Adhesion. 可调疏水性基底可实现最佳细胞粘附效果

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-08-23 DOI: 10.1002/mabi.202400196

Yuriy Snyder, Mary Todd, Soumen Jana

{"title":"Substrates with Tunable Hydrophobicity for Optimal Cell Adhesion.","authors":"Yuriy Snyder, Mary Todd, Soumen Jana","doi":"10.1002/mabi.202400196","DOIUrl":"https://doi.org/10.1002/mabi.202400196","url":null,"abstract":"Electrospinning is a technique used to create nano/micro-fibrous materials from various polymers for biomedical uses. Polymers like polycaprolactone (PCL) are commonly used, but their hydrophobic properties can limit their applications. To enhance hydrophilicity, nonionic surfactants such as sorbitane monooleate (Span80) and poloxamer (P188) can be added to the PCL electrospinning solution without altering its net charge density. These additions enable the successful production of PCL/P188 and PCL/Span80 fibrous substrates. In this study, P188 and Span80 are incorporated into the PCL solutions; they are successfully electrospun into PCL/P188 and PCL/Span80 substrates, respectively. PCL/P188 substrates show that until a specific P188 concentration, fiber and pore sizes are similar to PCL substrates. However, exceeding 0.30% P188 concentration enlarges fibers, impacting fiber uniformity at higher concentrations. Conversely, higher concentrations of Span80 result in thicker, less uniform fibers, indicating potential disruptions in the electrospinning process. Notably, both surfactants significantly improve substrate hydrophilicity, enhancing the adhesion and proliferation of fibroblasts, endothelial cells, and smooth muscle cells. P188, in particular, shows superior efficacy in promoting cell adhesion and growth at concentrations optimized for different cell types. Therefore, precise surfactant concentrations in the electrospinning solution can lead to the optimization of electrospun substrates for tissue engineering applications.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142036257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Acryloylated and Methacryloylated Nanocellulose Derivatives with Improved Mucoadhesive Properties. 新型丙烯酰化和甲基丙烯酰化纳米纤维素衍生物具有更好的黏附性。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-08-23 DOI: 10.1002/mabi.202400183

Abdumutolib A Atakhanov, Nurbek Sh Ashurov, Makhliyo M Kuzieva, Burhon N Mamadiyorov, Doniyor J Ergashev, Sayyora Sh Rashidova, Vitaliy V Khutoryanskiy

{"title":"Novel Acryloylated and Methacryloylated Nanocellulose Derivatives with Improved Mucoadhesive Properties.","authors":"Abdumutolib A Atakhanov, Nurbek Sh Ashurov, Makhliyo M Kuzieva, Burhon N Mamadiyorov, Doniyor J Ergashev, Sayyora Sh Rashidova, Vitaliy V Khutoryanskiy","doi":"10.1002/mabi.202400183","DOIUrl":"https://doi.org/10.1002/mabi.202400183","url":null,"abstract":"In this work, three nanocellulose derivatives are synthesized with the aim of preparing new mucoadhesive materials. Nanocellulose is reacted with glycidyl methacrylate in dimethylsulphoxide, and with acryloyl and methacryloyl chloride in dimethylacetamide in the presence of 4-(N,N-dimethylamino)pyridine as a catalyst. These reactions are carried out under heterogeneous conditions, and the reaction products are characterized using various spectroscopic techniques, X-ray diffraction, atomic force microscopy, and thermogravimetric analysis. The Fourier-transform infrared spectra showed all the characteristic absorption bands typical for cellulose and also new peaks at 1720 cm-1 for the carbonyl group (C═O) and 1639, 812 cm-1 for the double bond (C═C). It is established that the crystal structure of the nanocellulose is slightly changed with derivatisation and the thermal stability of these derivatives increased. Mucoadhesive properties of nanocellulose and its derivatives is evaluated using the tensile test, rotating basket method, and fluorescence flow-through method. The retention of these polymers is evaluated on sheep oral mucosal tissue ex vivo using artificial saliva. Test results demonstrated that the new derivatives of nanocellulose have improved mucoadhesive properties compared to the parent nanocellulose.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142036256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioactive Hydrogels Inspired by Laminin: An Emerging Biomaterial for Tissue Engineering Applications. 受层粘蛋白启发的生物活性水凝胶：用于组织工程应用的新兴生物材料

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-08-22 DOI: 10.1002/mabi.202400207

Sweta Mohanty, Sangita Roy

{"title":"Bioactive Hydrogels Inspired by Laminin: An Emerging Biomaterial for Tissue Engineering Applications.","authors":"Sweta Mohanty, Sangita Roy","doi":"10.1002/mabi.202400207","DOIUrl":"https://doi.org/10.1002/mabi.202400207","url":null,"abstract":"Tissue or organ damage due to severe injuries or chronic diseases can adversely affect the quality of life. Current treatments rely on organ or tissue transplantation which has limitations including unavailability of donors, ethical issues, or immune rejection after transplantations. These limitations can be addressed by tissue regeneration which involves the development of bioactive scaffolds closely mimicking the extracellular matrix (ECM). One of the major components of ECM is the laminin protein which supports several tissues associated with important organs. In this direction, peptide-based hydrogels can effectively mimic the essential characteristics of laminin. While several reports have discussed the structure of laminin, the potential of laminin-derived peptide hydrogels as effective biomaterial for tissue engineering applications is yet to be discussed. In this context, the current review focuses on the structure of laminin and its role as an essential ECM protein. Further, the potential of short peptide hydrogels in mimicking the crucial properties of laminin is proposed. The review further highlights the significance of bioactive hydrogels inspired by laminin - in addressing numerous tissue engineering applications including angiogenesis, neural, skeletal muscle, liver, and adipose tissue regeneration along with a brief outlook on the future applications of these laminin-based hydrogels.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142017959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On the Development of Polylactic Acid/Polycaprolactone Blended Films with High Retention Capacity. 开发具有高保留能力的聚乳酸/聚己内酯混合薄膜。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-08-18 DOI: 10.1002/mabi.202400272

Martina Cozzani, Pier Francesco Ferrari, Giacomo Damonte, Alessandro Pellis, Orietta Monticelli

{"title":"On the Development of Polylactic Acid/Polycaprolactone Blended Films with High Retention Capacity.","authors":"Martina Cozzani, Pier Francesco Ferrari, Giacomo Damonte, Alessandro Pellis, Orietta Monticelli","doi":"10.1002/mabi.202400272","DOIUrl":"https://doi.org/10.1002/mabi.202400272","url":null,"abstract":"The retention capacity of polymers is related to the development of systems that combine high surface-to-volume ratio with good handling and specific functionality. Biodegradability and biocompatibility are also key features for extending the field of applications to areas such as biomedicine. With this in mind, the aim of this work is to develop biodegradable, biocompatible, and highly functionalized porous films, that ensure suitable handling and a good surface-to-volume ratio. Polylactic acid (PLA) is applied as a polymer matrix to which a polycaprolactone with a star-shaped architecture (PCL-COOH) to ensure a high concentration of carboxylic end functionalities is added. The porous films are prepared using the phase inversion technique, which, as shown by Scanning Electron Microscopy (SEM) analysis, promotes good dispersion of the PCL-COOH domains. Absorption and release measurements performed with a positively charged model molecule show that the retention capacity and release rate can be tuned by changing the PCL-COOH concentration in the systems. Moreover, the adsorption properties for the formulation with the highest PCL-COOH content are also demonstrated with a real and widely used drug, namely doxorubicin. Finally, the bio- and hemocompatibility of the films, which are enzymatically degradable, are evaluated by using human keratinocytes and red blood cells, respectively.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142000376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Masthead: Macromol. Biosci. 8/2024 刊头：Macromol.Biosci.8/2024

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-08-14 DOI: 10.1002/mabi.202470019

引用次数: 0

Dual-Functional Implant Based on Gellan-Xanthan Hydrogel with Diopside, BMP-2 and Lysostaphin for Bone Defect Repair and Control of Staphylococcal Infection. 基于含有地奥赛德、BMP-2 和溶血素的结冷黄原水凝胶的双功能植入体，用于骨缺损修复和葡萄球菌感染控制。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-08-14 DOI: 10.1002/mabi.202400205

Anna S Karyagina, Alexander V Grishin, Alina G Kudinova, Inna N Bulygina, Elizaveta V Koudan, Polina A Orlova, Vera P Datsenko, Anna V Zhulina, Tatyana M Grunina, Maria S Poponova, Mikhail S Krivozubov, Maria S Gromova, Natalia V Strukova, Maria S Generalova, Kirill E Nikitin, Igor V Shchetinin, Lev O Luchnikov, Svetlana V Zaitseva, Maria A Kirsanova, Eugene S Statnik, Fedor S Senatov, Vladimir G Lunin, Alexander V Gromov

{"title":"Dual-Functional Implant Based on Gellan-Xanthan Hydrogel with Diopside, BMP-2 and Lysostaphin for Bone Defect Repair and Control of Staphylococcal Infection.","authors":"Anna S Karyagina, Alexander V Grishin, Alina G Kudinova, Inna N Bulygina, Elizaveta V Koudan, Polina A Orlova, Vera P Datsenko, Anna V Zhulina, Tatyana M Grunina, Maria S Poponova, Mikhail S Krivozubov, Maria S Gromova, Natalia V Strukova, Maria S Generalova, Kirill E Nikitin, Igor V Shchetinin, Lev O Luchnikov, Svetlana V Zaitseva, Maria A Kirsanova, Eugene S Statnik, Fedor S Senatov, Vladimir G Lunin, Alexander V Gromov","doi":"10.1002/mabi.202400205","DOIUrl":"https://doi.org/10.1002/mabi.202400205","url":null,"abstract":"A new dual-functional implant based on gellan-xanthan hydrogel with calcium-magnesium silicate ceramic diopside and recombinant lysostaphin and bone morphogenetic protein 2 (BMP-2)-ray is developed. In this composite, BMP-2 is immobilized on microparticles of diopside while lysostaphin is mixed directly into the hydrogel, providing sustained release of BMP-2 to allow gradual bone formation and rapid release of lysostaphin to eliminate infection immediately after implantation. Introduction of diopside of up to 3% (w/v) has a negligible effect on the mechanical properties of the hydrogel but provides a high sorption capacity for BMP-2. The hydrogels show good biocompatibility and antibacterial activity. Lysostaphin released from the implants over a 3 h period efficiently kills planktonic cells and completely destroys 24 h pre-formed biofilms of Staphylococcus aureus. Furthermore, in vivo experiments in a mouse model of critically-sized cranial defects infected with S. aureus show a complete lack of osteogenesis when implants contain only BMP-2, whereas, in the presence of lysostaphin, complete closure of the defect with newly formed mineralized bone tissue is observed. Thus, the new implantable gellan-xanthan hydrogel with diopside and recombinant lysostaphin and BMP-2 shows both osteogenic and antibacterial properties and represents a promising material for the treatment and/or prevention of osteomyelitis after bone trauma.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141976098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanoclay Hydrogel Microspheres with a Sandwich-Like Structure for Complex Tissue Infection Treatment 用于治疗复杂组织感染的具有三明治结构的纳米粘土水凝胶微球

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-08-14 DOI: 10.1002/mabi.202470018

Kunyuan Han, Jishizhan Chen, Qinglin Han, Lei Sun, Xieping Dong, Gengqiang Shi, Runhuai Yang, Wenqing Wei, Yunzhang Cheng

引用次数: 0