Macromolecular bioscience最新文献

{"title":"Dual-Functional ¹⁰B-Doped Carbon Dots Enabling Glucose-Based Cancer Cell Recognition and Boron Neutron Capture Therapy.","authors":"Huanhuan Guo, Tianyuan Zhong, Miao Pang, Xinyi Fang, Yong Pan, Yubin Huang","doi":"10.1002/mabi.70185","DOIUrl":"10.1002/mabi.70185","url":null,"abstract":"<p><p>Boron neutron capture therapy (BNCT) is a tumor-selective radiotherapy whose efficacy critically depends on efficient and preferential delivery of ¹⁰B to cancer cells. Meanwhile, accurate early cancer diagnosis remains challenging due to subtle intracellular differences between malignant and normal cells. Herein, we report a multifunctional theranostic platform based on boron-doped carbon dots (BCDs) that integrates cancer cell recognition with BNCT. Owing to the presence of boronic functionalities, the BCDs display enzyme-free, selective, and interference-resistant fluorescence enhancement in response to glucose, allowing reliable discrimination of cancer cells with elevated glucose uptake from normal cells. Upon neutron irradiation, BCDs exhibit potent cytotoxicity against B16-F10 melanoma cells in vitro and achieve significant tumor growth suppression in murine melanoma models without systemic toxicity. Furthermore, the intrinsic fluorescence of BCDs enables real-time tracking of ¹⁰B biodistribution through cellular and ex vivo tissue imaging. This work establishes a novel theranostic paradigm by innovatively integrating fluorescence-based cancer recognition into BNCT research, offering new design principles for next-generation multifunctional BNCT agents and advancing integrated cancer diagnostics and therapy.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"26 4","pages":"e70185"},"PeriodicalIF":4.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147722767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and In Vitro Characterization of Light Responsive Zinc-Based Nanoparticles Embedded in Collagen Sheets Intended for Wound Care Oriented Applications.","authors":"Nunzia Gallo, Simona Bettini, Alessia Nito, Giorgia Iaconisi, Francesca Russo, Alessandra Quarta, Loredana Capobianco, Ludovico Valli, Antonio Pennetta, Giuseppe Egidio De Benedetto, Alessandro Sannino, Luca Salvatore","doi":"10.1002/mabi.70188","DOIUrl":"10.1002/mabi.70188","url":null,"abstract":"<p><p>Bacterial infections are one of the most critical issues in hard-to-heal wounds that delay healing. The use of dressings able to both stimulate tissue regeneration and limit bacterial proliferation would reduce post-operative therapies and hospitalization costs. To this, antibacterial wound dressings have been developed. Among antibacterial agents, Zinc and Silver-based nanoparticles (NPs) are the most commonly used for their well-known safety profile. Among biomaterials, collagen is recognized as the gold standard for wound dressing manufacturing because of its unique pro-regenerative properties. With the aim of improving current wound dressings efficacy, a collagen-based device with light-responsive antibacterial properties was developed and in vitro characterized. A fibrillar type I collagen from horse tendon doped with patented Zinc-based NPs was employed for the manufacturing of thin sheets. After the analysis of the chemical-physical properties of collagen-based sheets, the preliminary evaluation of their antibacterial efficacy confirmed their effectiveness and light-responsiveness. The developed innovation would make it possible to in situ control the bacterial population and to reduce healing times and related costs.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"26 4","pages":"e70188"},"PeriodicalIF":4.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13113222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147775540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress in the Application of Hydrogel Materials for the Treatment of Intestinal Fistula.","authors":"Renjie Li, Fengya Jing, Tao Liu, Anbei Chen, Junsheng Li, Tianzhu Zhang","doi":"10.1002/mabi.70186","DOIUrl":"10.1002/mabi.70186","url":null,"abstract":"<p><p>Intestinal fistula refers to an abnormal anatomical channel between the intestines or between the intestine and another organ, commonly resulting from factors such as intestinal incision leakage, anastomotic leak, inflammatory bowel disease, and radiation enteropathy. Following its occurrence, various complications may arise, including abdominal infection, sepsis, intra-abdominal hypertension, massive abdominal hemorrhage, necrotizing fasciitis of the abdominal wall, chronic critical illness, and multiple organ dysfunction syndrome. In most cases, surgical resection of the intestinal fistula combined with thorough irrigation and drainage is required, though healing remains challenging. Hydrogels, as cross-linked polymer materials, are widely used in pharmaceuticals, biomedical implants, tissue engineering, regenerative medicine, and bioadhesive barriers. Based on these characteristics and functions, hydrogel materials also play a significant role in the treatment of intestinal fistulas. This review will systematically outline the design strategies, application mechanisms, and research progress of hydrogel materials for treating intestinal fistulas, and discuss current challenges as well as future development trends.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"26 4","pages":"e70186"},"PeriodicalIF":4.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147723062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface Functionalization of Iron Oxide Nanoparticles With Polymers and Small Molecules: Synthesis, Characterization, and Evaluation of Magnetic Hyperthermia Performance","authors":"Mehak,&nbsp;Mhonyamo M Patton,&nbsp;Jyoti Prasad Borah,&nbsp;Rajkumar P Thummer,&nbsp;Lalit Mohan Pandey","doi":"10.1002/mabi.202500632","DOIUrl":"10.1002/mabi.202500632","url":null,"abstract":"<div>\u0000 \u0000 <p>A systematic investigation of various surface coatings on iron oxide nanoparticles (IONPs) was conducted to understand their influence on interfacial interactions, colloidal stability, and magnetic performance. The surface-modified IONPs were synthesized via in situ and physical coating routes using synthetic polymers (polyvinylpyrrolidone (PVP), polyvinyl alcohol (PVA), polyethene glycol (PEG), polyethylenimine (PEI)), natural polymers (chitosan (CS), carboxymethyl cellulose (CMC), hyaluronic acid (HA)), small molecules (citric acid (CA), and oleic acid (OA)), and a silane coupling agent (3-aminopropyltriethoxysilane (APTES)). Face-centred cubic spinel-structured magnetite (Fe₃O₄) nanoparticles were synthesized with a lattice parameter of 8.356 Å, a particle size of 62 nm, and a saturation magnetization (M_s) of 67.95 A m² kg⁻¹. The coating of IONPs was confirmed by X-ray Photoelectron Spectroscopy (XPS) and Fourier Transform Infrared (FTIR) spectroscopy, indicating the formation of coordination bonds, hydrogen bonds, and electrostatic interactions between the NPs and the polymeric shell. The coatings substantially reduced agglomeration, thereby decreasing the particle size to 19–30 nm, while maintaining nearly superparamagnetic behavior at room temperature. The magnetic behavior was governed by Néel relaxation for NPs with a size less than 25 nm, whereas larger particles exhibited Brownian relaxation dominance. The relaxation mechanism is responsible for the heating output, measured as the Specific absorption rate (SAR), which provides a useful metric for comparing the heating efficiency of NPs. Induction heating studies carried out under the alternating magnetic field strength of 12.89 kA m⁻¹ and frequency of 336 kHz revealed that coated IONPs exhibited superior heating performance with improved SAR of 112 to 225 W g⁻¹. PEG, CMC, PEI, and APTES-coated IONPs achieved the hyperthermia temperature range of 42–46°C. However, PEG, CMC, and PEI-coated IONPs exhibited Néel relaxation dominance, which is desirable for in vivo hyperthermia applications. Furthermore, all the coated IONPs (except PEI-IONPs) demonstrated excellent in vitro cytocompatibility (&gt; 80%) with the human embryonic kidney (HEK293) cell line at 2 mg mL⁻¹ for 72 h. Simulated hyperthermic conditions led to cancer cell lethality, with normal cells being largely viable, suggesting the efficacy of hyperthermia for cancer treatment.</p>\u0000 </div>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"26 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147529972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-Functionality of Polysaccharide-based Hemostatic Powder: Effective Bleeding Control and Adhesion Reduction in Surgical Models","authors":"Lukasz Szymanski,&nbsp;Patrycja Krakowiak,&nbsp;Zuzanna Walas,&nbsp;Izabela Szubarczyk,&nbsp;Damian Matak","doi":"10.1002/mabi.202500664","DOIUrl":"10.1002/mabi.202500664","url":null,"abstract":"<div>\u0000 \u0000 <p>Postoperative adhesions are a frequent and serious complication of abdominal and pelvic surgeries, leading to chronic pain, infertility, and intestinal obstruction, with significant clinical, and economic burdens. While barrier agents are widely used for prevention, current solutions often suffer from limited efficacy and short retention times. This study evaluates the dual-functionality of a polysaccharide-based medical device, 4SEAL Hemostatic Powder, by evaluating hemostatic performance and the reduction of postoperative adhesions. Using rat models, the hemostatic efficacy of 4SEAL was assessed via a standardized liver incision protocol, while its anti-adhesive properties were compared to hyaluronic acid and saline controls following ovariohysterectomy. Outcomes measured included time to hemostasis, number and severity of adhesions, and comprehensive histological, hematological, biochemical, and metabolic analyses to assess safety and tissue response. In addition, the absorption profile of 4SEAL, in both gel and powder forms, was evaluated using a murine model. 4SEAL Hemostatic Powder significantly shortened time to hemostasis (2.14±0.39 min vs. 4.30±2.02 min for untreated controls, <i>p</i> = 0.003) and decreased both the number of adhesions (powder: 0.85±0.75, gel: 1.0±0.92 vs. 2.9±1.29 for controls, <i>p</i>&lt;0.01) and adhesion severity (powder: 3.75±2.67, gel: 3.55±3.19 vs. 7.7±4.88 for controls, <i>p</i>&lt;0.05). Hyaluronic acid reduced adhesion number (1.6±2.27, <i>p</i>&lt;0.05) but not severity (4.8±4.44, not statistically significant). Histological analysis indicated more advanced tissue regeneration and lower levels of fibrosis in 4SEAL-treated animals. No significant systemic biochemical or metabolic alterations were observed in the 4SEAL groups, supporting its biocompatibility. The findings indicate that 4SEAL Hemostatic Powder is effective in promoting hemostasis and reducing postoperative adhesion formation in preclinical models. The material demonstrated biocompatibility and biodegradability, supporting its potential utility as a dual-function surgical adjunct.</p>\u0000 </div>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"26 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147530035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microenvironment-Driven Closed-Loop Wound Management Based on Glycyrrhizic Acid/Gelatin/Zinc Sulfide Hydrogel","authors":"Shufan Wan,&nbsp;Limei Zhao,&nbsp;Lei Dai,&nbsp;Jinming Fang,&nbsp;Rongxian Bai,&nbsp;Yiyun Jiang,&nbsp;Bin Zou,&nbsp;Jiangtao Su,&nbsp;Hongmei Sun","doi":"10.1002/mabi.70176","DOIUrl":"10.1002/mabi.70176","url":null,"abstract":"<div>\u0000 \u0000 <p>Wound healing is a complex physiological process with different demands at different stages. In this work, a pH-responsive GA/Gelatin/ZnS (Glycyrrhizic Acid/Gelatin/Zinc Sulfide) hydrogel that can automatically release drugs was constructed in a facile way. It was fabricated by amide bonds, hydrogen bonds, and metal ion coordination among ZnS nanoparticles, glycyrrhizic acid (GA), and gelatin, which was confirmed by the molecular dynamics (MD) simulations and characterization results. Taking advantage of the pathological microenvironments at the wound site, it can synergize with multi-substance therapies at different stages, thus promoting wound healing. The as-prepared hydrogel can not only exhibit excellent coagulation ability in the hemostatic phase, but also release zinc ion (Zn²⁺) and hydrogen sulfide (H₂S) due to the weak acid microenvironment at the wound site, thus boosting inflammatory stage to proliferative phase. Meanwhile, benefit from its good re-epithelialization and angiogenesis properties in proliferative phase, the as-prepared hydrogel can increase collagen deposition during the remodeling phase, thereby accelerating wound healing. It would open a new perspective for effective wound management.</p>\u0000 </div>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"26 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147513363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shaping Micelles, Targeting Cancer: The Power of Block Copolymer Architectures","authors":"Seojin Park,&nbsp;Gayatri Patel,&nbsp;Bandana Padhan,&nbsp;Joyjyoti Das,&nbsp;Ramanujam Brahmadesam Thoopul Srinivasa Raghava,&nbsp;Madhumita Patel","doi":"10.1002/mabi.70174","DOIUrl":"10.1002/mabi.70174","url":null,"abstract":"<div>\u0000 \u0000 <p>Targeted micellar systems based on block copolymer micelles (BCMs) have become promising nanocarriers for cancer treatment because they can encapsulate hydrophobic drugs, improve pharmacokinetics, and facilitate tumor-specific delivery. BCMs, created through the self-assembly of amphiphilic block copolymers, are designed to bypass various biological barriers. This review discusses the multifaceted strategies employed by BCMs to overcome biological barriers that often impede therapeutic effectiveness. We first discuss the concept of BCMs, followed by an exploration of critical mechanisms, including opsonization, vascular flow dynamics, and the unique characteristics of the tumor microenvironment that facilitate their penetration and accumulation at tumor sites. Additionally, we examine various therapeutic applications of BCMs in cancer treatment, focusing on specific types such as amphiphilic copolymers, pluronics, and zwitterionic systems. Furthermore, this review covers the clinical nanoformulations of polymeric micelles. By leveraging the diverse properties of block copolymers, we highlight their potential to revolutionize targeted cancer therapies, addressing challenges in drug delivery and improving future outcomes.</p>\u0000 </div>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"26 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147513278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bimodal Plasmonic Devices Reveal Extensive Collagen Deposition in Mesenchymal Stem Cells Cultured on 3D Self-Assembled Peptide Scaffolds via a Birefringence-Induced Colorimetric Response","authors":"Christopher J.H. Chong,&nbsp;Vernise J.T. Lim,&nbsp;Mirren Charnley,&nbsp;Julian Ratcliffe,&nbsp;Emily H. Field,&nbsp;Lilith M. Caballero-Aguilar,&nbsp;Chad Johnson,&nbsp;Jacqueline M. Orian,&nbsp;Kristian Caracciolo,&nbsp;Eugeniu Balaur,&nbsp;Simon E. Moulton,&nbsp;Katrina J. Binger,&nbsp;Brian Abbey,&nbsp;Nicholas P. Reynolds","doi":"10.1002/mabi.202500372","DOIUrl":"10.1002/mabi.202500372","url":null,"abstract":"<div>\u0000 \u0000 <p>Tissue engineering holds promise for the treatment of osteoarthritis (OA), where protective hydrogel scaffolds have been combined with mesenchymal stem cells (MSCs) to promote chondrogenesis. Quantification of chondrogenesis by MSCs in 3D culture requires the imaging and detection of deposited extracellular matrix (ECM) components like collagen and proteoglycans. ECM protein quantification should be performed in a non-destructive, label-free, and simple manner. Here, we demonstrate a nanoplasmonic colorimetric device for the imaging of collagen requiring only a simple optical microscope. MSCs were encapsulated in the hydrogel-forming peptide Fmoc-diphenylalanine (Fmoc-FF) with arginine glycine aspartic acid (RGD) added. We showed, by colorimetric histology, that increased concentrations of RGD resulted in a significant increase in collagen deposition after 21 days. Traditional techniques such as immunohistological staining were not able to detect any RGD dependent increases in ECM deposition. Through an in-depth biophysical analysis we were able to correlate elevated RGD with enhanced cell-viability, collagen deposition, and reduced hydrogel stability. In summary, plasmon-enhanced colorimetric histology provides a non-destructive, label-free means to image collagen without resorting to destructive sample processing and complex immunohistological staining. This approach holds broad potential for routine quantification of collagen-rich biomaterials, promising widespread applications across research and clinical settings.</p>\u0000 </div>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"26 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145588029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of Double-Network Hydrogel Based on N,O-carboxymethyl chitosan, Aldehyde Hyaluronate, and Alginate for 3D Bio-Ink Applications","authors":"Binh Thanh Vu,&nbsp;Tuan-Ngan Tang,&nbsp;Dai-Tin Luong,&nbsp;Thao Thi-Phuong Nguyen,&nbsp;Yen-Nhi Ha-Nguyen,&nbsp;Nhu-Thuy Trinh,&nbsp;Hang Thi-Thuy Cao,&nbsp;Thi-Hiep Nguyen","doi":"10.1002/mabi.202500527","DOIUrl":"10.1002/mabi.202500527","url":null,"abstract":"<div>\u0000 \u0000 <p>This study fabricates and characterizes novel double-network hydrogels for 3D bio-ink applications, combining <i>N,O</i>-carboxymethyl chitosan (NOCC), aldehyde hyaluronate (AHy), and alginate (Alg). The initial Schiff base-cross-linked network (NOCC–AHy–Alg) is enhanced with a second ionic Ca²⁺ cross-linking, optimized via an internal suspension method (CaCO₃/glucono-δ-lactone). Optimal parameters are 0.2 <span>m</span> CaCO₃ and 0.1 <span>m</span> glucono-δ-lactone with a 1-h immersion, proving superior to external CaCl₂ immersion. This internal approach yields a more robust network with improved compressive strength, dimensional stability, and lower porosity, requiring sustained Ca²⁺ for long-term stability. The hydrogels also demonstrate excellent self-recovery and energy dissipation, indicating potential for 3D printing. In vitro studies confirm biocompatibility with L929 and AT-MSCs. Overall, these double-network hydrogels show significant promise as advanced bio-inks for tissue engineering and other biomedical uses.</p>\u0000 </div>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"26 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145804826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Osteoconductive Properties of Quince Seed Hydrocolloid-Based Composite Scaffolds Enriched with Bioactive Glass for Bone Tissue Engineering","authors":"Hilal Deniz Yilmaz-Dagdeviren,&nbsp;Kai Zheng,&nbsp;Aldo Roberto Boccaccini,&nbsp;Ahu Arslan Yildiz","doi":"10.1002/mabi.202500431","DOIUrl":"10.1002/mabi.202500431","url":null,"abstract":"<div>\u0000 \u0000 <p>Bioactive composite scaffolds enhance osteoconduction and mineralization, offering potential for bone regeneration. In this study, polysaccharide-based Quince Seed Hydrocolloid (QSH) was combined with Gelatin (Gel), mesoporous bioactive glass nanoparticles (MBGNs), and 45S5 bioactive glass (BG) to fabricate osteoconductive scaffolds. QSH/Gel/BG and QSH/Gel/MBGN composites were characterized for chemical composition, mechanical behavior, and in vitro bioactivity. FTIR and SEM-elemental mapping confirmed homogeneous bioactive glass incorporation. BET analysis revealed a &gt;3-fold increase in surface area for MBGN-containing scaffolds compared to BG and pristine QSH/Gel samples, attributed to the nanoscale mesoporous structure of MBGNs. Swelling tests showed a hydrophilic nature in all scaffolds, with MBGN composites exhibiting the highest swelling ratio (2094 ± 571%), nearly twice that of BG composites (1105 ± 56%). Compression tests indicated similar elastic moduli for MBGN and BG containing scaffolds (2330 and 2140 Pa). Human osteosarcoma cell cultures (28 days) demonstrated high viability (&gt;70%) and osteoconductive response in all composites. Alizarin Red staining and SEM mapping revealed greater mineral accumulation in MBGN-containing scaffolds (Ca/P: 2.53). Overall, both composites supported a 3D osteoconductive microenvironment, while MBGN scaffolds exhibited superior long-term cell viability and mineralization potential, emphasizing their suitability for bone tissue engineering applications.</p>\u0000 </div>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"26 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145768445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}