Macromolecular bioscience最新文献

{"title":"Development of Injectable and Self-Healing Gelatin/Dextran/Tannic Acid Composite Hydrogels Incorporating PCL/β-Tricalcium Phosphate Microspheres for Bone Tissue Regeneration.","authors":"Mohammadreza Ghaffarlou, Busra Kilic, Alkin Ozgen, Halil Murat Aydin","doi":"10.1002/mabi.202500298","DOIUrl":"https://doi.org/10.1002/mabi.202500298","url":null,"abstract":"<p><p>The current study introduces a novel hybrid system of polycaprolactone-nano beta-tricalcium phosphate microspheres (PCL-β-TCP Ms) combined with a hydrogel, which acts as a bone scaffold to accelerate osteogenic capabilities. This innovative system comprises a gelatin (Gel), oxidized dextran (Odex), and tannic acid (TA) hydrogel that integrates PCL-β-TCP microspheres. The Schiff base reaction between Gel and Odex, and the hydrogen bonding interaction of tannic acid and polymers, developed the hydrogel substrate. The process of fabricating the β-TCP-encapsulated PCL microspheres involved using the emulsion solvent evaporation technique, a method that allows for the encapsulation of bioactive substances within the microspheres. The findings revealed that incorporating microsphere-encapsulated β-TCP into the hydrogels notably enhanced their rheological properties, contributing to improved flow behavior and structural integrity. Additionally, the scanning electron microscopy (SEM) images illustrate that the addition of tannic acid leads to the development of a prominent fibrous structure within the hydrogels. This structural enhancement indicates that the presence of tannic acid plays a crucial role in modifying the hydrogel's composition at a microscopic level. The study investigated the interactions between biological cells and hybrid hydrogels in an in vitro setting. The viability and cytotoxicity testing demonstrated no adverse effects of the hybrid system (Gel/Odex/TA/PCL-β-TCP) and significantly improved preosteoblast cell (MC3T3-E1) viability. Moreover, the addition of these microspheres indicated a favorable environment for cell growth and development. Furthermore, Gel/Odex/TA/6%PCL-β-TCP Ms and Gel/Odex/TA/4%PCL-β-TCP Ms hydrogels exhibited a significant increase in calcium deposition and alkaline phosphatase (ALP) activity, respectively. These results reinforce that this multifunctional composite hydrogel may serve as a promising scaffold for bone tissue regeneration.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00298"},"PeriodicalIF":4.1,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145033742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scaffold-Free Extrusion-Based 3D Bioprinting of Cornea Constructs Using a Decellularized Corneal Extracellular Matrix Based Bioink and Human Placenta-Derived Mesenchymal Stem Cells.","authors":"Hugo A Marin-Tapia, Lorena Romero-Salazar, Miguel Mayorga-Rojas, Juan Carlos Arteaga-Arcos","doi":"10.1002/mabi.202500276","DOIUrl":"https://doi.org/10.1002/mabi.202500276","url":null,"abstract":"<p><p>The development of bioinks tailored for corneal tissue engineering is crucial to replicating the native structure and function of the cornea. This study presents a scaffold-free extrusion-based 3D bioprinting (E3DB) approach to fabricate cornea constructs without support materials or molds. Bioinks composed of decellularized corneal extracellular matrix (dCECM), sodium alginate (SA), and type B gelatin (TBG) were formulated and evaluated for rheological performance, including viscosity, shear thinning, and viscoelasticity. Among the tested formulations, bioink 3G10 (SA: 3%, dCECM: 6/mL, TBG: 10%; 2:1:1 ratio) demonstrated optimal rheological and printability performance, enabling the fabrication of stable, curvature-preserving constructs. The printed constructs exhibited high shape fidelity, light transmittance comparable to native cornea, and Young's modulus values within the physiological range. Human placenta-derived mesenchymal stem cells (hPMSCs) encapsulated in bioink 3G10 showed high initial viability, a transient decline at day 7, and recovery by day 14, accompanied by morphological elongation. Gene expression analysis revealed marked upregulation of keratocyte-specific markers (KERA and ALDH) and suppression of ACTA2, indicating progression toward a keratocyte-like phenotype. These findings underscore the suitability of hPMSCs and dCECM-based bioinks for scaffold-free cornea bioprinting, providing a robust platform for the development of anatomically accurate and biologically functional corneal grafts.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00276"},"PeriodicalIF":4.1,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145033801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to \"Hyaluronic Acid (HA)-Based Silk Fibroin/Zinc Oxide Core-Shell Electrospun Dressing for Burn Wound Management\".","authors":"Zhina Hadisi, Mehdi Farokhi, Hamid Reza Bakhsheshi-Rad, Maryam Jahanshahi, Sadegh Hasanpour, Erik Pagan, Alireza Dolatshahi-Pirouz, Yu Shrike Zhang, Subhas C Kundu, Mohsen Akbari","doi":"10.1002/mabi.202500366","DOIUrl":"https://doi.org/10.1002/mabi.202500366","url":null,"abstract":"","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00366"},"PeriodicalIF":4.1,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145033796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Luminescent Electro-Spun Nanofibers Crosslinked with Boronic Esters Exhibiting Controlled Release of Carbon Dots for Detection of Wound pHs and Enhanced Antimicrobial.","authors":"Nishadi Dilkushi Lokuge, Sofia Nieves Casillas-Popova, Prerna Singh, Adryanne Clermont-Paquette, Cameron D Skinner, Brandon L Findlay, Rafik Naccache, Jung Kwon Oh","doi":"10.1002/mabi.202500352","DOIUrl":"https://doi.org/10.1002/mabi.202500352","url":null,"abstract":"<p><p>Timely and accurate assessment of wounds during the healing process is crucial for proper diagnosis and treatment. Conventional wound dressings lack both real-time monitoring capabilities and active therapeutic functionalities, limiting their effectiveness in dynamic wound environments. Herein, we report our proof-of-concept approach exploring the unique emission properties and antimicrobial activities of carbon nanodots (CNDs) for simultaneous detection and treatment of bacteria. This approach centers on the fabrication of well-defined CND-embedded poly(vinyl alcohol) (PVA) e-spun nanofibrous mats, which are crosslinked with degradable boronic ester (BE) crosslinks. The BE-CND/PVA mats exhibit stimuli-responsive degradation to pHs and hydrogen peroxide as well as pH-responsive release of CNDs. Promisingly, the mats turn out to be hemocompatible with blood and biocompatible with skin cells. Furthermore, they exhibit notable antimicrobial activity against Gram-negative bacteria and demonstrate great potential for real-time monitoring of wound pH to assess the wound status. These results suggest that BE-CND/PVA mats could significantly enhance wound healing by providing localized therapeutic action, reducing the risk of bacterial infections, and enabling non-invasive monitoring of wound progress.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00352"},"PeriodicalIF":4.1,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"rev-Gelatin: A Gelatin with Reverse Thermo-Responsive Behavior Inspired by Candy and Ice Cubes Phase Dynamics.","authors":"Yeongjin Lee, Yu Ri Nam, Keumyeon Kim, Seongyeon Jo, Chanwoo Park, Jeehee Lee, Eunu Kim, Hong Kee Kim, Haeshin Lee","doi":"10.1002/mabi.202500144","DOIUrl":"https://doi.org/10.1002/mabi.202500144","url":null,"abstract":"<p><p>Conventional gelatin's gel-to-sol transition upon heating restricts its utility in biomedical applications that benefit from a gel state at physiological temperatures such as Pluronic F127 and poly(NIPAAm). Herein, we present \"rev-Gelatin\", a gelatin engineered with reverse thermo-responsive properties that undergoes a sol-to-gel transition as temperature rises from ambient to body temperature. Inspired by the phase dynamics of common materials like candy and ice cubes, whose surfaces soften or partially melt under warming, facilitating inter-object adhesion- rev-Gelatin leverages this concept to achieve fluidity at room temperature for easy injectability. At ambient temperature, rev-Gelatin exists as a microgel solution with sufficient fluidity in the sol state. However, upon exposure to elevated temperatures approaching physiological temperature, rev-Gelatin microgels coalesce through surface melting, forming a stable gel. This sol-to-gel transition is especially advantageous for hemostatic applications. Upon contact with blood, the temperature elevation induces rapid gelation of rev-Gelatin, effectively creating a barrier that reduces bleeding time and blood loss. Additionally, rev-Gelatin shows promise as a submucosal injection agent for gastrointestinal surgeries, making it a new class of thermo-sensitive biomaterials.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00144"},"PeriodicalIF":4.1,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Receptor-Binding Domain (RBD)-Loaded PEG-PCL Nanoparticle Formulations Against SARS-CoV-2.","authors":"Sena Ayçiçek Can, Selin Göksever, Umut Can Öz, Berrin Küçüktürkmen, Emrah Şefik Abamor, Mehmet İnan, Asuman Bozkır","doi":"10.1002/mabi.202500397","DOIUrl":"https://doi.org/10.1002/mabi.202500397","url":null,"abstract":"<p><p>The COVID-19 pandemic caused by the novel coronavirus SARS-CoV-2 has highlighted the critical need for safe and effective vaccines. In this study, subunit nanovaccine formulations were developed using the receptor-binding domain (RBD) of the SARS-CoV-2 spike (S) protein encapsulated in polymeric nanoparticles composed of poly(ethylene glycol)-block-poly(ε-caprolactone) (PEG-PCL). Two surfactants, poly(vinyl alcohol) (PVA) and sodium cholate (SC), were evaluated during formulation via a modified water-in-oil-in-water (w₁/o/w₂) emulsion-solvent evaporation method. The resulting nanoparticles were characterized for particle size, surface charge, encapsulation efficiency, and morphology. Optimized nanoparticles exhibited sizes below 300 nm, polydispersity indices less than 0.3, surface charges between ±10-20 mV, and encapsulation efficiencies exceeding 80%. SDS-PAGE confirmed structural integrity of the RBD, while in vitro release studies demonstrated sustained antigen release over time. Cellular response was assessed by measuring nitric oxide (NO) levels in dendritic cells, indicating comparable levels of cellular activation for both PVA- and SC-containing formulations. These findings demonstrate the potential of PEG-PCL-based nanovaccine systems for safe and stable delivery of viral antigens, offering a promising strategy for future vaccine development against COVID-19 and related pathogens.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00397"},"PeriodicalIF":4.1,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional Scaffold Biosensor and Drug Delivery System for Bacterial Infection Prevention During Skin Wound Healing.","authors":"Leonor Resina, Pau Caballero, Grant Guggenbiller, Andrew C Weems, Maria M Pérez-Madrigal, Carlos Alemán","doi":"10.1002/mabi.202500247","DOIUrl":"https://doi.org/10.1002/mabi.202500247","url":null,"abstract":"<p><p>This study investigates a multifunctional hydrogel system integrating carboxymethyl cellulose (CMC) in a 3D-printed limonene (LIM) scaffold coated with poly(3,4-ethylenedioxythiophene): polystyrene sulfonate (PEDOT:PSS). The system allows to enhance wound healing, prevent infections, and monitor the healing progress. CMC is crosslinked with citric acid (CA) to form the hydrogel matrix (CMC-CA), while the 3D-printed limonene (LIM) scaffold is embedded within the hydrogel to provide mechanical support. PEDOT:PSS and curcumin-loaded PEDOT (PEDOT:CUR) nanoparticles are integrated into the hydrogel-membrane system for electrochemical detection of bacterial infection and controlled delivery of the antibacterial drug. The CMC-CA hydrogel exhibits excellent mechanical properties, suitable for conforming to irregular wound surfaces. In addition to provide additional mechanical support, the LIM scaffold is used as a pillar for the incorporation of PEDOT The integration of PEDOT:PSS and PEDOT:CUR enable not only real-time monitoring of bacterial growth but also the electrostimulated release of curcumin, which demonstrates antibacterial activity against Escherichia coli and Staphylococcus aureus. Electrostimulation of the CMC-CA/LIM/PEDOT system promotes cell proliferation, supporting accelerated wound healing. In conclusion, the CMC-CA/LIM/PEDOT system combines mechanical support, infection monitoring, and enhanced healing through controlled drug delivery and electrical stimulation, addressing critical challenges in wound management.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00247"},"PeriodicalIF":4.1,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immiscible Phase Separation-Driven Microfabrication of Gelatin Methacryloyl Scaffolds for BMP-2 Delivery and Osteogenic Enhancement.","authors":"Basel A Khader, Stephen D Waldman, Dae Kun Hwang","doi":"10.1002/mabi.202500296","DOIUrl":"10.1002/mabi.202500296","url":null,"abstract":"<p><p>Gelatin methacryloyl (GelMA) hydrogels are recognized for their biocompatibility, tunable mechanics, and ability to support cellular functions, making them attractive for tissue engineering. However, achieving uniform, structurally stable micro-scaffolds for minimally invasive delivery remains challenging. Injectable hydrogels provide targeted delivery but lack the micro-architectural complexity required for effective regeneration, while 3D printing offers precision yet faces resolution, handling, and mechanical limitations. To overcome these barriers, we developed injectable GelMA micro-scaffolds (mS-GelMA) with controlled porosity, stability, and reproducibility using stop-flow lithography (SFL). This technique enables precise control over shape, porosity, and degradation, surpassing conventional injection moulding and 3D bioprinting in micro-particle uniformity and reproducibility. Scaffold performance was optimized by incorporating trimethylolpropane triacrylate (TMPTA) into GelMA, enhancing drug delivery and regenerative potential. Cellular assays confirmed high biocompatibility and functionality, with human mesenchymal stem cells (hMSCs) exhibiting excellent viability, migration, and osteogenic differentiation within the mS-GelMA scaffolds. These findings demonstrate that SFL-fabricated GelMA scaffolds bridge the gap between injectability and structural complexity, offering a promising platform for minimally invasive tissue engineering. This work highlights the potential of SFL-engineered hydrogels to advance scaffold-based regenerative medicine by combining architectural precision, biological performance, and clinical applicability.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00296"},"PeriodicalIF":4.1,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144959474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formulation and In Vitro Assessment of a Hybrid Gel-Microparticle Composite for Ocular Delivery of Dual-Antiglaucoma Drugs.","authors":"Subrat Kumar Panigrahi, Debasmita Saha, Sougat Das, Saptarshi Majumdar, Lopamudra Giri","doi":"10.1002/mabi.202500270","DOIUrl":"https://doi.org/10.1002/mabi.202500270","url":null,"abstract":"<p><p>Glaucoma, a major global health issue, is the second leading cause of blindness. Topical eye drops are commonly used due to their simplicity, but the eye's protective barriers hinder optimal drug concentration at the target site. This study addresses these challenges by developing a novel dual-drug delivery system, integrating polycaprolactone microparticles loaded with latanoprost(hydrophobic) and timolol maleate(hydrophilic) antiglaucoma drugs into a gelatin-alginate hydrogel matrix. There is a fundamental challenge to combine both drugs in the same delivery system with a controlled release profile. Hydrogel-microparticles(HMPs) were assessed via in vitro drug-release and cell culture for biocompatibility with Raman analysis for chemical compatibility and drug diffusivity. Results showed that the hydrogel-microparticle system has prolonged drug release for up to 32 days. Raman analysis confirmed the chemical compatibility of the formulation components, and in vitro biocompatibility studies demonstrate that the HMPs system is biocompatible and exhibits minimal toxicity to the cells. This novel HMPs system can serve as a flexible, controlled release platform modulating the release profile. Our study highlights that the polymer and drug properties, along with the external matrix, were key factors influencing the drug release behavior of the formulations, and the proposed HMPs system can potentially be considered for glaucoma therapy.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00270"},"PeriodicalIF":4.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144959390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Biophysical and -Chemical Cues of Crosslinked Gelatin Networks on Endothelial Cell Adhesion, Morphology, Proliferation, and Angiogenic Signaling.","authors":"Laurens Parmentier, Louis Van der Meeren, Andre G Skirtach, Sandra Van Vlierberghe","doi":"10.1002/mabi.202400579","DOIUrl":"https://doi.org/10.1002/mabi.202400579","url":null,"abstract":"<p><p>Pre-vascularization through endothelial cell seeding within 3D-bioprinted constructs holds great promise to advance tissue engineering vascularization strategies. Herein, the effect of biophysical (bulk modulus (0.50-76.09 kPa), local elasticity (3.65-53.68 kPa), roughness (421.07-858.30 nm)) and biochemical (0, 25 ng/mL VEGF-A or 3 mg/mL adenosine) cues in widely used bioinks (methacryloyl vs. thiol-norbornene modified gelatins incorporating 6.21-25.81 mM crosslinked moieties) was systematically investigated on the adhesion, morphology, proliferation and angiogenic signaling of seeded human umbilical vein endothelial cells. Chain-growth networks exhibited an enhanced roughness together with the need for a higher concentration of converted moieties to obtain a non-statistically significant local substrate elasticity compared to the step-growth substrates (5 w/v% GelNBSH DS 74/67 vs. 5 w/v% GelMA DS 67: 6.02 ± 2.94 vs. 3.85 ± 1.24 kPa with 6.21 vs. 7.33 mM crosslinked moieties). Despite bulk compressive moduli with insignificant difference (5 w/v% GelNBSH DS 74/67 vs. 5 w/v% GelMA DS 99: 13.57 ± 0.56 vs. 14.18 ± 1.59 kPa), the 5 w/v% GelNBSH DS 74/67 networks outperformed the 5 w/v% GelMA DS 99 samples, especially through enhanced early and more mature angiogenic signaling (Ang-2, HB-EGF, VEGF-C, FGF-1, IL-8, Endothelin-1) after 1 day of culture, while chain-growth networks required VEGF-A supplementation to attain similar signaling.</p>","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00579"},"PeriodicalIF":4.1,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144959441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}