Macromolecular bioscience最新文献_第3页

Influence of Biophysical and -Chemical Cues of Crosslinked Gelatin Networks on Endothelial Cell Adhesion, Morphology, Proliferation, and Angiogenic Signaling. 交联明胶网络的生物物理和化学线索对内皮细胞粘附、形态、增殖和血管生成信号的影响。

IF 4.1 4区医学

Macromolecular bioscience Pub Date : 2025-08-29 DOI: 10.1002/mabi.202400579

Laurens Parmentier, Louis Van der Meeren, Andre G Skirtach, Sandra Van Vlierberghe

{"title":"Influence of Biophysical and -Chemical Cues of Crosslinked Gelatin Networks on Endothelial Cell Adhesion, Morphology, Proliferation, and Angiogenic Signaling.","authors":"Laurens Parmentier, Louis Van der Meeren, Andre G Skirtach, Sandra Van Vlierberghe","doi":"10.1002/mabi.202400579","DOIUrl":"https://doi.org/10.1002/mabi.202400579","url":null,"abstract":"Pre-vascularization through endothelial cell seeding within 3D-bioprinted constructs holds great promise to advance tissue engineering vascularization strategies. Herein, the effect of biophysical (bulk modulus (0.50-76.09 kPa), local elasticity (3.65-53.68 kPa), roughness (421.07-858.30 nm)) and biochemical (0, 25 ng/mL VEGF-A or 3 mg/mL adenosine) cues in widely used bioinks (methacryloyl vs. thiol-norbornene modified gelatins incorporating 6.21-25.81 mM crosslinked moieties) was systematically investigated on the adhesion, morphology, proliferation and angiogenic signaling of seeded human umbilical vein endothelial cells. Chain-growth networks exhibited an enhanced roughness together with the need for a higher concentration of converted moieties to obtain a non-statistically significant local substrate elasticity compared to the step-growth substrates (5 w/v% GelNBSH DS 74/67 vs. 5 w/v% GelMA DS 67: 6.02 ± 2.94 vs. 3.85 ± 1.24 kPa with 6.21 vs. 7.33 mM crosslinked moieties). Despite bulk compressive moduli with insignificant difference (5 w/v% GelNBSH DS 74/67 vs. 5 w/v% GelMA DS 99: 13.57 ± 0.56 vs. 14.18 ± 1.59 kPa), the 5 w/v% GelNBSH DS 74/67 networks outperformed the 5 w/v% GelMA DS 99 samples, especially through enhanced early and more mature angiogenic signaling (Ang-2, HB-EGF, VEGF-C, FGF-1, IL-8, Endothelin-1) after 1 day of culture, while chain-growth networks required VEGF-A supplementation to attain similar signaling.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00579"},"PeriodicalIF":4.1,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144959441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasound-Responsive Drug Delivery System for Cancer Therapy. 用于癌症治疗的超声反应药物输送系统。

IF 4.1 4区医学

Macromolecular bioscience Pub Date : 2025-08-28 DOI: 10.1002/mabi.202500082

Han Wang, Yuning Liu, Yao Li, Yanjun Zhao, Xin Li

{"title":"Ultrasound-Responsive Drug Delivery System for Cancer Therapy.","authors":"Han Wang, Yuning Liu, Yao Li, Yanjun Zhao, Xin Li","doi":"10.1002/mabi.202500082","DOIUrl":"https://doi.org/10.1002/mabi.202500082","url":null,"abstract":"Ultrasound-responsive drug delivery systems have emerged as a promising approach in cancer therapy, offering enhanced targeting precision, controlled drug release, and reduced systemic toxicity. These systems utilize the mechanical and thermal effects of ultrasound to enable the spatiotemporally triggered release of therapeutic payloads in tumor sites. This review provides an overview of the key mechanisms underlying ultrasound-responsive drug delivery, including the activation of sonosensitizers and prodrugs, as well as the role of ultrasound-responsive nanocarriers such as liposomes, micelles, nanobubbles, and metal-organic frameworks. We explore the biophysical effects of ultrasound, including mechanical cavitation and thermal effects, that enable localized drug release and their application in enhancing the permeability of tumor tissues. Additionally, the combination of ultrasound with other therapeutic modalities such as chemotherapy, immunotherapy, and gene therapy is discussed, highlighting the synergistic potential of multimodal treatment strategies. Despite the promising preclinical findings, challenges remain, such as optimizing ultrasound parameters, improving nanocarrier stability, and ensuring clinical translation. Future research is directed toward overcoming these limitations and expanding the clinical applicability of ultrasound-responsive drug delivery systems in cancer treatment. Integrating ultrasound-triggered systems with advanced imaging technologies offers a pathway toward precision medicine, allowing for tailored cancer therapies with minimized off-target effects.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00082"},"PeriodicalIF":4.1,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144959477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Integrity Nanoformulation of Resiquimod (R848) for Dual Autophagy Activation and PD-L1 Modulation in Triple-Negative Breast Cancer. 雷西喹莫特（R848）用于三阴性乳腺癌双自噬激活和PD-L1调节的高完整性纳米制剂

IF 4.1 4区医学

Macromolecular bioscience Pub Date : 2025-08-28 DOI: 10.1002/mabi.202500388

Ahmed Salem, Sara Attia, Samah El-Ghlban, A S Montaser, Mohamed F Abdelhameed, Maged W Helmy, Mohamed F Attia

{"title":"High-Integrity Nanoformulation of Resiquimod (R848) for Dual Autophagy Activation and PD-L1 Modulation in Triple-Negative Breast Cancer.","authors":"Ahmed Salem, Sara Attia, Samah El-Ghlban, A S Montaser, Mohamed F Abdelhameed, Maged W Helmy, Mohamed F Attia","doi":"10.1002/mabi.202500388","DOIUrl":"https://doi.org/10.1002/mabi.202500388","url":null,"abstract":"Triple-negative breast cancer (TNBC) remains a formidable clinical challenge due to its molecular heterogeneity and resistance to conventional therapies. This study presents a high-integrity nanoemulsion (NE) formulation designed to enhance the delivery and stability of the Toll-like receptor 7/8 (TLR7/8) agonist resiquimod (R848) for immunotherapy. Neutral and negatively charged NEs were developed with and without the reactive lipophilic compound ricinoleic acid. Physicochemical characterization and in vitro studies in RAW 264.7 macrophages and 4T1 TNBC cells demonstrated that R848-loaded NEs exhibit prolonged shelf-life, minimal protein binding, and efficient cellular uptake. Incorporation of ricinoleic acid improved drug retention and delivery, likely through enhanced drug-lipid interactions. Molecular profiling in 4T1 cells revealed modulation of key biomarkers (TLR4/7, Cyclin D1, NF-κB), induction of autophagy (LC3II, p62, Beclin-1), and upregulation of PD-L1 expression. These dual effects, autophagy-mediated antitumor mechanisms and immune checkpoint modulation, highlight the potential of R848-NEs as a synergistic partner in anti-PD-L1 combination therapy, offering a promising strategy for TNBC treatment.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00388"},"PeriodicalIF":4.1,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144959448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and Characterization of Cell Wall and Extracellular Polysaccharides from Cultures of the Mycoparasitic Strain Trichoderma koningiopsis. koningiopsis木霉菌细胞壁及胞外多糖的分离与鉴定。

IF 4.1 4区医学

Macromolecular bioscience Pub Date : 2025-08-26 DOI: 10.1002/mabi.202500032

Artur Nowak, Kamila Wlizło, Iwona Komaniecka, Monika Szymańska-Chargot, Artur Zdunek, Justyna Kapral-Piotrowska, Marek Stankevič, Katarzyna Tyśkiewicz, Jolanta Jaroszuk-Ściseł

{"title":"Isolation and Characterization of Cell Wall and Extracellular Polysaccharides from Cultures of the Mycoparasitic Strain Trichoderma koningiopsis.","authors":"Artur Nowak, Kamila Wlizło, Iwona Komaniecka, Monika Szymańska-Chargot, Artur Zdunek, Justyna Kapral-Piotrowska, Marek Stankevič, Katarzyna Tyśkiewicz, Jolanta Jaroszuk-Ściseł","doi":"10.1002/mabi.202500032","DOIUrl":"https://doi.org/10.1002/mabi.202500032","url":null,"abstract":"Using Response Surface Methodology (RSM) to optimise cultivation conditions showed that Trichoderma koningiopsis EPS biosynthesis depended on an alkaline pH (>9.0) and a high nitrogen concentration (≥20 g/L). This resulted in a yield increase of over 60% compared to the control conditions. Three wall polymer fractions were extracted from the obtained biomass: cold water soluble (WPSZ), hot water soluble (WPSC), and alkali soluble (WPSNaOH). These accounted for 13.3, 1.8, and 20.2% of the dry weight of the mycelium, respectively. Structural analyses revealed that the obtained EPS was mannan, with the WPS fractions consisting predominantly of (1→4)-Glc residues branching at the (1→3,6) and (1→4,6) positions. FT-IR and FT-Raman analyses revealed that α-bonds predominated in the WPSZ and WPSC fractions, whereas β-bonds predominated in the EPS and WPSNaOH fractions. This was confirmed by NMR analysis. The obtained polymer fractions (PS) exhibited antioxidant properties using the ABTS, DPPH, and FRAP methods, as well as the ability to bind bisphenol A from an aqueous environment. The most significant property of PS polymers is their ability to reduce germination and inhibit mycelial growth of the phytopathogenic Fusarium culmorum strain. These polymers exhibit various bioactive properties and have potential applications in many areas of human life.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00032"},"PeriodicalIF":4.1,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144959411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Antimicrobial Divalent Metal Cations Onto Oxidized Surface of Polyhydroxyalkanoate Films on Biodegradability in Seawater. 抗菌二价金属阳离子对聚羟基烷酸盐膜氧化表面生物降解性的影响。

IF 4.1 4区医学

Macromolecular bioscience Pub Date : 2025-08-26 DOI: 10.1002/mabi.202500162

Jobu Tateiwa, Yu-I Hsu, Hiroshi Uyama, Takeharu Tsuge, Tadahisa Iwata

{"title":"Effect of Antimicrobial Divalent Metal Cations Onto Oxidized Surface of Polyhydroxyalkanoate Films on Biodegradability in Seawater.","authors":"Jobu Tateiwa, Yu-I Hsu, Hiroshi Uyama, Takeharu Tsuge, Tadahisa Iwata","doi":"10.1002/mabi.202500162","DOIUrl":"https://doi.org/10.1002/mabi.202500162","url":null,"abstract":"Poly[(R)-3-hydroxybutyrare-co-(R)-3-hydroxypivalate] (P(3HB-co-3HPi)) films, a type of polyhydroxyalkanoate (PHA), are oxidized using photoactivated chlorine dioxide radical (ClO2•) gas to generate carboxyl groups and loaded with divalent metal cations, including Cu2+, Zn2+, and Ca2+ ions, via ionic interactions. The P(3HB-co-3HPi) films loaded with Cu2+ ions exhibit enhanced antibacterial activity against Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli) compared with untreated P(3HB-co-3HPi) films. In seawater, the biodegradation of these Cu2+ and Zn2+-loaded films is initially inhibited by the antimicrobial activity of the cations and occurs gradually; therefore, loading antimicrobial divalent metal cations onto the surface of PHAs inhibits biodegradation in seawater temporarily but allows biodegradation to occur with time. These results indicate that PHAs could be employed in seawater without undergoing biodegradation, such that PHAs could be used in fishing gear, including fishing lines that are repeatedly exposed to seawater for short periods.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00162"},"PeriodicalIF":4.1,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144959468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidics-Generated Cryo-Fracture Resistant CNCs-Reinforced Composite Microcapsules for Enhanced Cryopreservation of Encapsulated Cells. 微流体生成的抗低温断裂cncs增强复合材料微胶囊用于增强被封装细胞的低温保存。

IF 4.1 4区医学

Macromolecular bioscience Pub Date : 2025-08-22 DOI: 10.1002/mabi.202500217

Marlene Davis Ekpo, George Frimpong Boafo, Yanmin Yang, Yimer Seid, Chuanpin Chen, Songwen Tan, Rongrong Wang, Zhen Huang

{"title":"Microfluidics-Generated Cryo-Fracture Resistant CNCs-Reinforced Composite Microcapsules for Enhanced Cryopreservation of Encapsulated Cells.","authors":"Marlene Davis Ekpo, George Frimpong Boafo, Yanmin Yang, Yimer Seid, Chuanpin Chen, Songwen Tan, Rongrong Wang, Zhen Huang","doi":"10.1002/mabi.202500217","DOIUrl":"https://doi.org/10.1002/mabi.202500217","url":null,"abstract":"Immunoisolated cells hold great promise for advancing cell therapeutics; however, their long-term storage remains a critical challenge due to ice formation and mechanical damage to both cells and the encapsulating matrix during cryopreservation. In this study, a cryo-fracture-resistant composite microcapsule has been developed using microfluidic technology, where sodium alginate was reinforced with cellulose nanocrystals (CNCs). The composite microcapsules were assessed for their ability to provide structural, mechanical, morphological, chemical, and thermal stability during cryopreservation. Additionally, red blood cells (RBCs) were used as a model to evaluate the ability of the optimized microcapsules to improve cell viability post-thaw. Results revealed that the microfluidic system allowed for precise control over microcapsule size and uniformity, which is crucial for even freezing and thawing. CNCs reinforced microcapsules exhibited delayed ice formation, improved ice recrystallization inhibition, and superior protection against freeze-induced deformations. Furthermore, the composite microcapsules enhanced post-thaw recovery of encapsulated RBCs and enabled the incorporation of a biocompatible cryoprotectant (trehalose). This study demonstrates a novel approach to optimizing cryopreservation techniques by leveraging the enhanced properties of composite microcapsules to mitigate cryo-injury and improve the functional recovery of encapsulated cells. These findings present a promising strategy for advancing microencapsulation-based cell therapies and broader biomedical applications.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00217"},"PeriodicalIF":4.1,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144959461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Antioxidant and Antibacterial Injectable Hydrogels Incorporating Clove Oil-Loaded Mesoporous Bioactive Glass Nanoparticles: A Promising Strategy for Enhanced Bone Regeneration. 含有丁香油负载的介孔生物活性玻璃纳米颗粒的新型抗氧化和抗菌注射水凝胶：一种增强骨再生的有前途的策略。

IF 4.1 4区医学

Macromolecular bioscience Pub Date : 2025-08-19 DOI: 10.1002/mabi.202500252

Andrada-Ioana Damian-Buda, Markus Lorke, Aldo R Boccaccini, Irem Unalan

{"title":"Novel Antioxidant and Antibacterial Injectable Hydrogels Incorporating Clove Oil-Loaded Mesoporous Bioactive Glass Nanoparticles: A Promising Strategy for Enhanced Bone Regeneration.","authors":"Andrada-Ioana Damian-Buda, Markus Lorke, Aldo R Boccaccini, Irem Unalan","doi":"10.1002/mabi.202500252","DOIUrl":"10.1002/mabi.202500252","url":null,"abstract":"Alginate (Alg)-gelatin (Gel)-xanthan gum (Xan) injectable hydrogels incorporating mesoporous bioactive glass nanoparticles (MBGNs) loaded with different concentrations of clove oil (MBGNs+CLV) were developed for bone regeneration applications. The hydrogels exhibited an interconnected network of pores, with their dimensions fulfilling the requirements for bone tissue engineering. MBGNs+CLV were successfully embedded within the Alg-Gel-Xan matrix. The presence of CLV in the hydrogel was proven by the total phenolic content (TPC), which was further correlated with the enhanced antioxidant activity, which increased with higher CLV concentrations. Simultaneously, the addition of MBGNs+CLV led to an increase of the hydrogel's Young's modulus, while maintaining its injectability and adhesion properties. Based on the CLV release profile, a burst release was followed by a sustained and controlled release, which was independent of the loaded CLV concentration. The drug release profiles were further correlated with the degradation study, revealing the possible mechanism of drug release. The MBGNs+CLV-based hydrogel possessed strong antibacterial properties and promoted MC3T3-E1 pre-osteoblast cell viability without altering cell morphology. In conclusion, our study highlights the potential of Alg-Gel-Xan containing MBGNs+CLV as an antioxidant injectable hydrogel with controlled and sustained CLV delivery, offering a promising strategy for stimulating bone regeneration and addressing bone infection and oxidative stress.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00252"},"PeriodicalIF":4.1,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineered Silk-Dressing for Accelerated Wound Healing: Biocompatibility and Efficacy Studies. 用于加速伤口愈合的工程丝绸敷料：生物相容性和功效研究。

IF 4.1 4区医学

Macromolecular bioscience Pub Date : 2025-08-17 DOI: 10.1002/mabi.202500323

Rucha Deshpande, Lakshmi R Pillai, Raeesa Sayyad, Swati Shukla, Anuya Nisal, Premnath Venugopalan

{"title":"Engineered Silk-Dressing for Accelerated Wound Healing: Biocompatibility and Efficacy Studies.","authors":"Rucha Deshpande, Lakshmi R Pillai, Raeesa Sayyad, Swati Shukla, Anuya Nisal, Premnath Venugopalan","doi":"10.1002/mabi.202500323","DOIUrl":"https://doi.org/10.1002/mabi.202500323","url":null,"abstract":"Advanced wound care dressings are essential for improving clinical outcomes. The present study investigates the wound management potential of a unique dressing fabricated from silk proteins. The dressing was characterized for its physical and structural properties, including surface texture, porosity, fluid absorption capacity, and moisture vapor transmission rate. These parameters have been found to be critical for optimal wound healing. In vivo full thickness wound healing studies in a rat model validated the efficacy of the Silk-dressing compared to conventional cotton gauze and commercial polyurethane foam dressings. Histopathological analysis confirmed improved re-epithelialization, collagen deposition, angiogenesis, and formation of secondary follicles. Key advantages of Silk-dressing included non-adherence, absorption of exudate, maintenance of optimal moisture at wound site and accelerated wound closure. Biocompatibility studies were also conducted in accordance with ISO 10993 guidelines, demonstrating no cytotoxicity, irritation, sensitization, or pyrogenicity. These findings highlight the potential of this uniquely designed Silk-dressing as a superior alternative for wound management, with a potential to improve clinical outcomes.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00323"},"PeriodicalIF":4.1,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Size-Dependent Elastic Modulus and Core-Shell Structural Characteristics of Electrospun Nanofibers. 静电纺纳米纤维的尺寸弹性模量和核壳结构特性。

IF 4.1 4区医学

Macromolecular bioscience Pub Date : 2025-08-17 DOI: 10.1002/mabi.202500280

Muhammad Azeem Munawar, Fritjof Nilsson, Dirk W Schubert

{"title":"Size-Dependent Elastic Modulus and Core-Shell Structural Characteristics of Electrospun Nanofibers.","authors":"Muhammad Azeem Munawar, Fritjof Nilsson, Dirk W Schubert","doi":"10.1002/mabi.202500280","DOIUrl":"https://doi.org/10.1002/mabi.202500280","url":null,"abstract":"This study investigates the size-dependent mechanical properties of electrospun polycaprolactone (PCL) nanofibers by analyzing the relationship between fiber diameter and Young's modulus. Experimental data reveal a clear inverse trend: as fiber diameter decreases, stiffness increases significantly, indicating strong surface and confinement effects at the nanoscale. Two theoretical models were employed to interpret the observed behavior: a simplified core-shell model (Model 1) and an extended model (Model 2) incorporating surface tension and curvature elasticity. Both models accurately fit the experimental data across a diameter range of 450-850 nm, with Model 2 providing slightly better agreement at intermediate diameters (∼600-750 nm), where surface mechanics become more prominent. The enhanced stiffness in thinner fibers is attributed to increased surface-to-volume ratio and tighter molecular packing, while larger fibers exhibit bulk-dominated mechanical responses. These findings highlight the importance of nanoscale geometry and surface effects in determining mechanical properties and suggest that fiber stiffness can be systematically tuned via diameter control during electrospinning.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00280"},"PeriodicalIF":4.1,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrophobic Drug Delivery into T Cells Using Carboxy-Terminal Phenylalanine-Modified Dendrigraft Polylysines. 利用羧基端苯基丙氨酸修饰的树突聚赖氨酸向T细胞递送疏水药物。

IF 4.1 4区医学

Macromolecular bioscience Pub Date : 2025-08-17 DOI: 10.1002/mabi.202500207

Chie Kojima, Akinobu Sakai, Tetsuya Kadonosono

{"title":"Hydrophobic Drug Delivery into T Cells Using Carboxy-Terminal Phenylalanine-Modified Dendrigraft Polylysines.","authors":"Chie Kojima, Akinobu Sakai, Tetsuya Kadonosono","doi":"10.1002/mabi.202500207","DOIUrl":"https://doi.org/10.1002/mabi.202500207","url":null,"abstract":"T cells in the lymph nodes play an important role in cancer immunotherapy. Dendrigraft polylysines (DGLs) are potent nanoplatforms used in nanomedicine. In the present study, DGLs were modified with 1,2-cyclohexanedicarboxylic acid (CHex) and phenylalanine (Phe) to produce DGL-CHex-Phe for drug delivery into T cells. Various DGL-CHex-Phe polymers were synthesized using different generations of DGL by reacting with Phe at different ratios. DGL-CHex-Phe polymers with a higher generation and more Phe efficiently associated with Jurkat cells, a T cell model. These polymers are internalized by T cells via an amino acid transporter and/or direct membrane association. The hydrophobic model drug, paclitaxel (PTX), was loaded onto the polymers. DGL(G3)-CHex-Phe93 loaded the most PTX molecules among them, and most of them were retained therein for 3 h. PTX-loaded polymers exhibited cytotoxic effects against Jurkat cells at a level similar to that of free PTX. DGL(G3)-CHex-Phe93 efficiently accumulated in lymph nodes after intradermal injection, which was partially co-localized with T cells. These results suggested that DGL(G3)-CHex-Phe93 is useful for the delivery of hydrophobic drugs into immune cells including T cells in lymph nodes.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00207"},"PeriodicalIF":4.1,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0