Macromolecular bioscience最新文献_第4页

Flexible, Electrochemical Skin‐Like Platform for Inflammatory Biomarker Monitoring 用于炎症生物标记物监测的灵活电化学类肤平台

IF 4.6 4区医学

Macromolecular bioscience Pub Date : 2024-09-18 DOI: 10.1002/mabi.202400287

Carolina Lourenço, Felismina Moreira, Rui Igreja, Gabriela Martins

{"title":"Flexible, Electrochemical Skin‐Like Platform for Inflammatory Biomarker Monitoring","authors":"Carolina Lourenço, Felismina Moreira, Rui Igreja, Gabriela Martins","doi":"10.1002/mabi.202400287","DOIUrl":"https://doi.org/10.1002/mabi.202400287","url":null,"abstract":"Addressing global challenges in wound management has greatly encouraged the emergence of home diagnosis and monitoring devices. This technological shift has accelerated the development of new skin patch sensors for continuous health monitoring. A key requirement is the creation of flexible platforms capable of mimicking human skin features. Here, for the first time, an innovative, highly adaptable electrochemical biosensor with molecularly imprinted polymers (MIPs) is customized for the detection of the inflammatory biomarker interleukin‐6 (IL‐6). The 3‐electrode gold pattern is geometrically standardized onto a 6 µm thick polyimide flexible membrane, an optically transparent, and biocompatible polymeric substrate. Subsequently, a biomimetic sensing layer specifically designed for the detection of IL‐6 target is produced on these transducers. The obtained MIP biosensor shows a good linear response within the concentration range 50 pg mL−1‐50 ng mL−1, with a low limit of detection (8 pg mL−1). X‐ray photoelectron spectroscopy, scanning electron microscopy, and Fourier transform infrared spectroscopy characterizations confirm the modifications of the flexible gold transducer. After optimization, the biosensing device shows remarkable potential in terms of sensitivity, selectivity, and reproducibility. Overall, the integration of a low‐cost electrochemical sensor on biocompatible flexible polymers opens the way for a new generation of monitoring tools with higher accuracy, less invasiveness, and greater patient comfort.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142260040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Masthead: Macromol. Biosci. 9/2024 刊头：Macromol.Biosci.9/2024

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-09-16 DOI: 10.1002/mabi.202470021

引用次数: 0

Viral Vector Eluting Lenses for Single-Step Targeted Expression of Genetically-Encoded Activity Sensors for in Vivo Microendoscopic Calcium Imaging. 用于体内显微内窥镜钙成像的基因编码活动传感器单步靶向表达的病毒载体洗脱透镜。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-09-16 DOI: 10.1002/mabi.202400359

Carolyn K Jons, David Cheng, Changxin Dong, Emily L Meany, Jonathan J Nassi, Eric A Appel

{"title":"Viral Vector Eluting Lenses for Single-Step Targeted Expression of Genetically-Encoded Activity Sensors for in Vivo Microendoscopic Calcium Imaging.","authors":"Carolyn K Jons, David Cheng, Changxin Dong, Emily L Meany, Jonathan J Nassi, Eric A Appel","doi":"10.1002/mabi.202400359","DOIUrl":"10.1002/mabi.202400359","url":null,"abstract":"Optical methods for studying the brain offer powerful approaches for understanding how neural activity underlies complex behavior. These methods typically rely on genetically encoded sensors and actuators to monitor and control neural activity. For microendoscopic calcium imaging, injection of a virus followed by implantation of a lens probe is required to express a calcium sensor and enable optical access to the target brain region. This two-step process poses several challenges, chief among them being the risks associated with mistargeting and/or misalignment between virus expression zone, lens probe and target brain region. Here, an adeno-associated virus (AAV)-eluting polymer coating is engineered for gradient refractive index (GRIN) lenses enabling the expression of a genetically encoded calcium indicator (GCaMP) directly within the brain region of interest upon implantation of the lens. This approach requires only one surgical step and guarantees alignment between GCaMP expression and lens in the brain. Additionally, the slow virus release from these coatings increases the working time for surgical implantation, expanding the brain regions and species amenable to this approach. These enhanced capabilities should accelerate neuroscience research utilizing optical methods and advance the understanding of the neural circuit mechanisms underlying brain function and behavior in health and disease.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142290419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

One Stone Several Birds: Self-Localizing Submicrocages With Dual Loading Points for Multifunctional Drug Delivery 一石数鸟：具有双装载点的自定位亚微囊用于多功能药物输送

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-09-16 DOI: 10.1002/mabi.202470020

Shuxuan Liu, Jifei Wang, Yong Jiang, Yao Wang, Bin Yang, Hao Li, Guofu Zhou

引用次数: 0

Unlocking the Potential: PEGylation and Molecular Weight Reduction of Ionenes for Enhanced Antifungal Activity and Biocompatibility 释放潜力：离子烯的 PEG 化和分子量降低可增强抗真菌活性和生物相容性

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-09-16 DOI: 10.1002/mabi.202470022

Jan M. Kurzyna, Rafał J. Kopiasz, Martyna Paul, Magdalena Flont, Patrycja Baranowska, Jolanta Mierzejewska, Karolina Drężek, Waldemar Tomaszewski, Elżbieta Jastrzębska, Dominik Jańczewski

引用次数: 0

Preparation, Structural Analysis, and Growth‐Promoting Effects of Amomum longiligulare Polysaccharide 1‐Mg (II) Complex 长日月藻多糖 1-Mg (II) 复合物的制备、结构分析和促进生长作用

IF 4.6 4区医学

Macromolecular bioscience Pub Date : 2024-09-13 DOI: 10.1002/mabi.202400297

Yijing Chen, Haowen Wu, Chenglong Zhang, Qiyuan Luo, Yun Chen

{"title":"Preparation, Structural Analysis, and Growth‐Promoting Effects of Amomum longiligulare Polysaccharide 1‐Mg (II) Complex","authors":"Yijing Chen, Haowen Wu, Chenglong Zhang, Qiyuan Luo, Yun Chen","doi":"10.1002/mabi.202400297","DOIUrl":"https://doi.org/10.1002/mabi.202400297","url":null,"abstract":"In this study, Amomum longiligulare polysaccharide 1 (ALP1) is used to chelate with magnesium (Mg) to synthesize the ALP1‐Mg (II) complex (ALP1‐Mg). Based on Box‐Behnken response surface design, the optimum technological conditions are 22 mg mL−1 trisodium citrate, 2.10 mol L−1 MgCl2, reaction at 70 °C for 2.9 h, resulting in a maximum Mg content of 2.13%. Next, the physicochemical properties and structural characteristics of ALP1 and ALP1‐Mg are characterized, and the results show that the morphology, conformation, crystallinity, and thermal stability of ALP1‐Mg are changed. In addition, dietary supplementation of 500 mg kg−1 ALP1‐Mg significantly reduces the feed conversion ratio during the grower (15–35 d). Meanwhile, the villus height/crypt depth of the duodenum and ileum are significantly increased, and the relative abundance of Lactobacillus is significantly elevated. Taken together, the results suggest that ALP1‐Mg is a potential growth‐promoting feed additive.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142260041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytotherapeutic Hierarchical PCL‐Based Scaffolds as a Multifunctional Wound Dressing: Combining 3D Printing and Electrospinning 以植物疗法为基础的分层 PCL 支架作为多功能伤口敷料：三维打印与电纺丝的结合

IF 4.6 4区医学

Macromolecular bioscience Pub Date : 2024-09-10 DOI: 10.1002/mabi.202400253

Irem Unalan, Benedikt Slavik, Andrea Buettner, Aldo R. Boccaccini

{"title":"Phytotherapeutic Hierarchical PCL‐Based Scaffolds as a Multifunctional Wound Dressing: Combining 3D Printing and Electrospinning","authors":"Irem Unalan, Benedikt Slavik, Andrea Buettner, Aldo R. Boccaccini","doi":"10.1002/mabi.202400253","DOIUrl":"https://doi.org/10.1002/mabi.202400253","url":null,"abstract":"This study focuses on developing hybrid scaffolds incorporating phytotherapeutic agents via a combination of three‐dimensional (3D) printing and electrospinning to enhance mechanical properties and provide antibacterial activity, in order to address the limitations of traditional antibiotics. In this regard, 3D‐printed polycaprolactone (PCL) struts are first fabricated using fused deposition modeling (FDM). Then, alkaline surface treatment is applied to improve the adhesion of electrospun nanofibers. Finally, peppermint oil (PEP) or clove oil (CLV)‐incorporated PCL‐gelatin (GEL) electrospun nanofibers are collected on top of the 3D‐printed PCL scaffolds by electrospinning. Incorporating PEP or CLV into PCL‐GEL electrospun nanofibers enhances the scaffold's layer detachment and adhesion force. In addition, the DPPH free radical scavenging activity assay indicates that incorporating PEP or CLV improves the antioxidant properties of the scaffolds. Further, antibacterial activity results reveal that PEP or CLV incorporated scaffolds exhibit inhibition against Staphylococcus aureus and Escherichia coli bacteria. Moreover, anti‐inflammatory assays show that scaffolds reduce the concentration of nitric oxide (NO) released from Raw 264.7 macrophage‐like cells. On the other hand, the phytotherapeutic hierarchical scaffolds have no toxic effect on normal human dermal fibroblast (NHDF) cells, and PEP or CLV enhance cell attachment and proliferation. Overall, incorporating natural phytotherapeutic agents into hierarchical scaffolds shows promise for advancing wound healing applications.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142182648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metals at the Helm: Revolutionizing Protein Assembly and Applications. 金属掌舵：彻底改变蛋白质组装和应用。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-09-06 DOI: 10.1002/mabi.202400126

Maoping Duan, Chenyan Lv, Jiachen Zang, Xiaojing Leng, Guanghua Zhao, Tuo Zhang

{"title":"Metals at the Helm: Revolutionizing Protein Assembly and Applications.","authors":"Maoping Duan, Chenyan Lv, Jiachen Zang, Xiaojing Leng, Guanghua Zhao, Tuo Zhang","doi":"10.1002/mabi.202400126","DOIUrl":"https://doi.org/10.1002/mabi.202400126","url":null,"abstract":"Protein assembly is an essential process in biological systems, where proteins self-assemble into complex structures with diverse functions. Inspired by the exquisite control over protein assembly in nature, scientists have been exploring ways to design and assemble protein structures with precise control over their topologies and functions. One promising approach for achieving this goal is through metal coordination, which utilizes metal-binding motifs to mediate protein-protein interactions and assemble protein complexes with controlled stoichiometry and geometry. Metal coordination provides a modular and tunable approach for protein assembly and de novo structure design, where the metal ion acts as a molecular glue that holds the protein subunits together in a specific orientation. Metal-coordinated protein assemblies have shown great potential for developing functional metalloproteinase, novel biomaterials and integrated drug delivery systems. In this review, an overview of the recent advances in protein assemblies benefited from metal coordination is provided, focusing on various protein arrangements in different dimensions including protein oligomers, protein nanocage and higher-order protein architectures. Moreover, the key metal-binding motifs and strategies used to assemble protein structures with precise control over their properties are highlighted. The potential applications of metal-mediated protein assemblies in biotechnology and biomedicine are also discussed.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142140490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bone-Mimetic Osteon Microtopographies on Poly-ε-Caprolactone Enhance the Osteogenic Potential of Human Mesenchymal Stem Cells. 聚ε-己内酯上的仿骨骨微结构可增强人间质干细胞的成骨潜能

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-09-05 DOI: 10.1002/mabi.202400311

Matthias Vostatek, Elettra Verin, Marvin Tamm, Mario Rothbauer, Stefan Toegel, Francesco Moscato

{"title":"Bone-Mimetic Osteon Microtopographies on Poly-ε-Caprolactone Enhance the Osteogenic Potential of Human Mesenchymal Stem Cells.","authors":"Matthias Vostatek, Elettra Verin, Marvin Tamm, Mario Rothbauer, Stefan Toegel, Francesco Moscato","doi":"10.1002/mabi.202400311","DOIUrl":"https://doi.org/10.1002/mabi.202400311","url":null,"abstract":"The attributes of implant surfaces are pivotal for successful osseointegration. Among surface engineering strategies, microtopography stands out as a promising approach to promote early cellular interactions. This study aims to design and craft a novel biomimetic osteon-like surface modification and to compare its impact on human mesenchymal stem cells (hMSCs) with four established topographies: blank, inverted pyramids, protrusions, and grooves. Poly-ε-caprolactone samples are fabricated using 2-photon-polymerization and soft lithography, prior to analysis via scanning electron microscopy (SEM), water contact angle (WCA), and protein adsorption assays. Additionally, cellular responses including cell attachment, proliferation, morphology, cytoskeletal organization, and osteogenic differentiation potential are evaluated. SEM confirms the successful fabrication of microtopographies, with minimal effect on WCA and protein adsorption. Cell attachment experiments demonstrate a significant increase on the osteon-like structure, being three times higher than on the blank. Proliferation assays indicate a fourfold increase with osteon-like microtopography compared to the blank, while ALP activity is notably elevated with osteon-like microtopography at days 7 (threefold increase over blank) and 14 (fivefold increase over blank). In conclusion, the novel biomimetic osteon-like structure demonstrates favorable responses from hMSCs, suggesting potential for promoting successful implant integration in vivo.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alginate Capsules: Versatile Applications and Production Techniques. 藻酸盐胶囊：多种应用和生产技术。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-09-05 DOI: 10.1002/mabi.202400202

Francesca Passannanti, Marianna Gallo, Giulia Lentini, Rosa Colucci Cante, Federica Nigro, Roberto Nigro, Andrea Budelli

引用次数: 0