Macromolecular bioscience最新文献_第4页

A Comparative Study between Thiol-Ene and Acrylate Photocrosslinkable Hyaluronic Acid Hydrogel Inks for Digital Light Processing. 数字光处理用硫醇烯与丙烯酸酯光交联透明质酸凝胶油墨的比较研究。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-12-31 DOI: 10.1002/mabi.202400535

Therese Steudter, Tobias Lam, Hamidreza Pirmahboub, Christian Stoppel, Lutz Kloke, Samuel Pearson, Aránzazu Del Campo

{"title":"A Comparative Study between Thiol-Ene and Acrylate Photocrosslinkable Hyaluronic Acid Hydrogel Inks for Digital Light Processing.","authors":"Therese Steudter, Tobias Lam, Hamidreza Pirmahboub, Christian Stoppel, Lutz Kloke, Samuel Pearson, Aránzazu Del Campo","doi":"10.1002/mabi.202400535","DOIUrl":"https://doi.org/10.1002/mabi.202400535","url":null,"abstract":"Photocrosslinkable formulations based on the radical thiol-ene reaction are considered better alternatives than methacrylated counterparts for light-based fabrication processes. This study quantifies differences between thiol-ene and methacrylated crosslinked hydrogels in terms of precursors stability, the control of the crosslinking process, and the resolution of printed features particularized for hyaluronic acid (HA) inks at concentrations relevant for bioprinting. First, the synthesis of HA functionalized with norbornene, allyl ether, or methacrylate groups with the same molecular weight and comparable degrees of functionalization is presented. The thiol-ene hydrogel precursors show storage stability over 15 months, 3.8 times higher than the methacrylated derivative. Photorheology experiments demonstrate up to 4.7-times faster photocrosslinking. Network formation in photoinitiated thiol-ene HA crosslinking allows higher temporal control than in methacrylated HA, which shows long post-illumination hardening. Using digital light processing, 4% w/v HA hydrogels crosslinked with a dithiol allowed printing of 13.5 × 4 × 1 mm3 layers with holes of 100 µm resolution within 2 s. This is the smallest feature size demonstrated in DLP printing with HA-based thiol-ene hydrogels. The results are important to estimate the extent to which the synthetic effort of introducing -ene functions can pay off in the printing step.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2400535"},"PeriodicalIF":4.4,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Crosslinking Approach for Biomanufacturing of a Collagen-Based Skin Dermal Template 一种新的交联方法用于胶原基皮肤模板的生物制造。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-12-23 DOI: 10.1002/mabi.202400457

Weng Wan Chan, Keya Rani Roy, Bach Quang Le, Hariharan Ezhilarasu, Xiaoqian Zhang, Ryan Yi Da Lim, Avinanda Banerjee, Mariya Kuriakose, Krystle Joy Ng, Priya Murugan, Chun Ting Goh, Weibiao Zhou, May Win Naing, Srikala Raghavan, Deepak Choudhury

{"title":"A Novel Crosslinking Approach for Biomanufacturing of a Collagen-Based Skin Dermal Template","authors":"Weng Wan Chan, Keya Rani Roy, Bach Quang Le, Hariharan Ezhilarasu, Xiaoqian Zhang, Ryan Yi Da Lim, Avinanda Banerjee, Mariya Kuriakose, Krystle Joy Ng, Priya Murugan, Chun Ting Goh, Weibiao Zhou, May Win Naing, Srikala Raghavan, Deepak Choudhury","doi":"10.1002/mabi.202400457","DOIUrl":"10.1002/mabi.202400457","url":null,"abstract":"Third-degree burns result in extensive damage to the skin's epidermal and dermal layers, with limited treatment options available. Currently, xenogeneic collagen-based skin grafts are used as scaffolds to integrate into the wound bed and provide a template for neodermis formation. Existing commercial products like Integra dermal templates rely on a time-consuming and variable dehydrothermal (DHT) crosslinking process. This study presents a novel crosslinking process for collagen sponges, utilizing UV irradiation followed by glutaraldehyde (GA) crosslinking. This UV method allows to fine-tune the template's crosslink density and degradation profile while significantly reducing the total crosslinking time from 48 to 24 h compared to DHT/GA crosslinking. In vitro characterization and in vivo validation are conducted using a full-thickness skin wound mouse model. The collagen template supports the human dermal fibroblast cell line WS-1 proliferation more effectively than the Integra template after 2 weeks in culture. Additionally, in vivo data indicate a similar level of regeneration of full-thickness skin wounds in mouse models between the sponge and Integra templates. Furthermore, the sponge template does not elicit any abnormal angiogenic or immune responses. The crosslinking approach offers a promising alternative production process for collagen sponge scaffolds.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"25 2","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanoscale Liposomes Co-Loaded with Irinotecan Hydrochloride and Thalidomide for Colorectal Cancer Synergistic Therapy. 纳米级脂质体联合盐酸伊立替康和沙利度胺用于大肠癌的协同治疗。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-12-20 DOI: 10.1002/mabi.202400478

Xuanrong Sun, Yubei Gong, Ting Xie, Zixi Fu, Dongze Lu, Bin Wei, Yue Cai, Wenlong Yao, Jie Shen

{"title":"Nanoscale Liposomes Co-Loaded with Irinotecan Hydrochloride and Thalidomide for Colorectal Cancer Synergistic Therapy.","authors":"Xuanrong Sun, Yubei Gong, Ting Xie, Zixi Fu, Dongze Lu, Bin Wei, Yue Cai, Wenlong Yao, Jie Shen","doi":"10.1002/mabi.202400478","DOIUrl":"https://doi.org/10.1002/mabi.202400478","url":null,"abstract":"Irinotecan hydrochloride (CPT-11) is one of the first-line drugs used in the clinical treatment of colorectal cancer (CRC). However, the concomitant adverse effect of delayed diarrhea has hindered its clinical use. CPT-11 combined with Thalidomide (THA) therapy is considered a palliative strategy. To optimize the synergistic treatment of CPT-11 and THA, co-loaded liposomes are constructed using cholesterol, lecithin, and 1, 2-Distearoyl-sn-glycero-3-phosphoethanolamine-Poly(ethylene glycol) (DSPE-PEG) as the \"immune and gut microbiota regulator.\" The co-loaded liposomes, which possess good stability, are prepared by the solvent injection method. After the treatment with the co-loaded liposomes, tumor growth in CRC-bearing mice is significantly inhibited. In particular, the co-loaded liposomes demonstrate favorable diarrhea-relieving effects through the modulation of inflammatory cytokines and gut microbiota. These findings suggest that the co-loaded liposomes have great potential as a combined drug-delivery platform for CRC therapy.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2400478"},"PeriodicalIF":4.4,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polymeric Microneedles for Transdermal Delivery of Human Placental Tissue for the Treatment of Osteoarthritis. 经皮给药人胎盘组织的聚合微针治疗骨关节炎。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-12-20 DOI: 10.1002/mabi.202400485

Jithu Jerin James, Suman Pahal, Anbu Jayaraman, Damodar Nayak A, Sridhar Koteshwar Narasimhachar, Sudarson Sundarrajan, Basavaraj Basappa Veerabhadraiah, Bharath Srinivasan, Praveen K Vemula, Thanh D Nguyen, Sandhya Kadamboor Veethil

{"title":"Polymeric Microneedles for Transdermal Delivery of Human Placental Tissue for the Treatment of Osteoarthritis.","authors":"Jithu Jerin James, Suman Pahal, Anbu Jayaraman, Damodar Nayak A, Sridhar Koteshwar Narasimhachar, Sudarson Sundarrajan, Basavaraj Basappa Veerabhadraiah, Bharath Srinivasan, Praveen K Vemula, Thanh D Nguyen, Sandhya Kadamboor Veethil","doi":"10.1002/mabi.202400485","DOIUrl":"https://doi.org/10.1002/mabi.202400485","url":null,"abstract":"Biologics targeting matrix-degrading proteases, cartilage repair, and inflammation are emerging as promising approaches for osteoarthritis (OA) treatment. Recent research highlights biologic-human placental tissue (HPT) as a potential OA therapy due to its biocompatibility, abundant protein biofactors, and ability to reduce cartilage degradation by suppressing protease expression. Microneedles (MNs) are receiving growing attention for enhancing transdermal delivery of biologics as an alternative to conventional subcutaneous injections. The lyophilized human placental extract (LHP) loaded polymeric MNs are fabricated using a micromolding technique for transdermal delivery. Ex vivo release studies reveal that MNs exhibit a gradual and consistent release of LHP, indicating a sustained delivery profile. LHP-MNs are nontoxic and anti-inflammatory in nature against human skin cells and interleukin (IL-1β) induced synovial cells. Furthermore, the in vivo study shows that LHP-MNs substantially improve behavioral parameters in OA rat models and lower serum concentrations of tumor necrosis factor- α (TNF-α) and cartilage oligomeric matrix protein (COMP) biomarkers, thereby alleviating knee and ankle joint injuries. Histopathological analysis indicates that LHP-MNs significantly preserve cartilage integrity. The study results suggest that employing polymeric MNs for transdermal delivery of LHP can be a promising treatment approach for OA, with the added benefit of excellent patient compliance.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2400485"},"PeriodicalIF":4.4,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical Signal-Induced Supramolecular Hydrogelation for Structured Free-Standing Soft Material Formation 生物化学信号诱导的结构独立软材料形成的超分子水凝胶

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-12-16 DOI: 10.1002/mabi.202470028

Dineshkumar Bharathidasan, Akshay Sunil Salvi, Suryasarathi Bose, Chandan Maity

引用次数: 0

Masthead: Macromol. Biosci. 12/2024

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-12-16 DOI: 10.1002/mabi.202470029

引用次数: 0

Engineering RAFT Polymers to the Protein-capped Gold Nanoclusters for Developing Fluorescent Polymeric Nanoconjugates. 工程RAFT聚合物到蛋白包覆金纳米簇用于开发荧光聚合物纳米偶联物。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-12-11 DOI: 10.1002/mabi.202400451

Raviteja Gaddala, Aruna Kumar Chelluboyina, Sonu Kumar

引用次数: 0

An Alginate/Gelatin Injectable Hydrogel Containing Au Nanoparticles for Transplantation of Embryonic Mouse Cardiomyocytes in Myocardial Repair 含金纳米颗粒海藻酸盐/明胶可注射水凝胶用于胚胎小鼠心肌细胞移植修复。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-12-11 DOI: 10.1002/mabi.202400301

Behnam Pournemati, Hadi Tabesh, Rouhollah Mehdinavaz Aghdam, Ali Hossein Rezayan, Ali Poorkhalil, Seyed Hossein Ahmadi Tafti, Asieh Heirani-Tabasi, Hossein Eyni, Marjan Malekmohamadi, Safieh Boroumand, Alessandra Pinna

{"title":"An Alginate/Gelatin Injectable Hydrogel Containing Au Nanoparticles for Transplantation of Embryonic Mouse Cardiomyocytes in Myocardial Repair","authors":"Behnam Pournemati, Hadi Tabesh, Rouhollah Mehdinavaz Aghdam, Ali Hossein Rezayan, Ali Poorkhalil, Seyed Hossein Ahmadi Tafti, Asieh Heirani-Tabasi, Hossein Eyni, Marjan Malekmohamadi, Safieh Boroumand, Alessandra Pinna","doi":"10.1002/mabi.202400301","DOIUrl":"10.1002/mabi.202400301","url":null,"abstract":"In advancing cardiac tissue engineering (CTE), the development of injectable hydrogels mirroring myocardial properties is pivotal. The designed hydrogels must not only support cardiac cell growth but also have to be conductive to properly promote the functionalities of cardiac cells. Here, a facile approach is developed to incorporate gold nanoparticles (AuNPs) into an injectable hydrogel composed of Alginate (Alg) and Gelatin (Gel). The resultant nanocomposite hydrogel boasts a porous interconnected network and superior conductivity (2.04 × 10–4 S cm−1) compared to the base Alg/Gel hydrogel. Hydrogel hydration and in vitro degradation profiles affirm their suitability as carriers for cardiac cells. Importantly, Alg/Gel+AuNPs hydrogels exhibit no toxicity to mouse Embryonic Cardiac Cells (mECCs) over 7 days, elevating connexin 43 (Cx43) and cardiac troponin T (CTnT) gene expression compared to controls. Then, the Alg/Gel+AuNPs hydrogel is used as a carrier for intramyocardial delivery of mECCs in rats with myocardial infarction. The significant increase in α-Smooth Muscle Actin (α-SMA) and cardiac troponin T (CTnT) expression along with the increase in ejection fraction (EF), smaller infarction size, less fibrosis area confirmed that the hydrogel efficiently promoted the transmission of mechanical and electrical signals between transplanted cells and surrounding tissue.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"25 2","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dexamethasone Acetate-Loaded PLGA Nanospheres Targeting Liver Macrophages 靶向肝巨噬细胞的醋酸地塞米松负载PLGA纳米球。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-11-29 DOI: 10.1002/mabi.202400411

Barbora Boltnarova, Anna Durinova, Lenka Jandova, Stanislav Micuda, Otto Kucera, Ivona Pavkova, Miloslav Machacek, Ivana Nemeckova, Marek Vojta, Jan Dusek, Maria Krutakova, Petr Nachtigal, Petr Pavek, Ondrej Holas

{"title":"Dexamethasone Acetate-Loaded PLGA Nanospheres Targeting Liver Macrophages","authors":"Barbora Boltnarova, Anna Durinova, Lenka Jandova, Stanislav Micuda, Otto Kucera, Ivona Pavkova, Miloslav Machacek, Ivana Nemeckova, Marek Vojta, Jan Dusek, Maria Krutakova, Petr Nachtigal, Petr Pavek, Ondrej Holas","doi":"10.1002/mabi.202400411","DOIUrl":"10.1002/mabi.202400411","url":null,"abstract":"Glucocorticoids are potent anti-inflammatory drugs, although their use is associated with severe side effects. Loading glucocorticoids into suitable nanocarriers can significantly reduce these undesirable effects. Macrophages play a crucial role in inflammation, making them strategic targets for glucocorticoid-loaded nanocarriers. The main objective of this study is to develop a glucocorticoid-loaded PLGA nanocarrier specifically targeting liver macrophages, thereby enabling the localized release of glucocorticoids at the site of inflammation. Dexamethasone acetate (DA)-loaded PLGA nanospheres designed for passive macrophage targeting are synthesized using the nanoprecipitation method. Two types of PLGA NSs in the size range of 100–300 nm are prepared, achieving a DA-loading efficiency of 19 %. Sustained DA release from nanospheres over 3 days is demonstrated. Flow cytometry analysis using murine bone marrow-derived macrophages demonstrates the efficient internalization of fluorescent dye-labeled PLGA nanospheres, particularly into pro-inflammatory macrophages. Significant down-regulation in pro-inflammatory cytokine genes mRNA is observed without apparent cytotoxicity after treatment with DA-loaded PLGA nanospheres. Subsequent experiments in mice confirm liver macrophage-specific nanospheres accumulation following intravenous administration using in vivo imaging, flow cytometry, and fluorescence microscopy. Taken together, the data show that the DA-loaded PLGA nanospheres are a promising drug-delivery system for the treatment of inflammatory liver diseases.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"25 2","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202400411","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142751320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrospinning of Cellulose Benzyl Carbamates for Enantioselective Membrane Filtration. 用于对映体选择性膜过滤的纤维素苄基氨基甲酸酯电纺丝。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-11-27 DOI: 10.1002/mabi.202400415

Steve Nono-Tagne, Thomas Heinze, Martin Gericke, Issei Otsuka

{"title":"Electrospinning of Cellulose Benzyl Carbamates for Enantioselective Membrane Filtration.","authors":"Steve Nono-Tagne, Thomas Heinze, Martin Gericke, Issei Otsuka","doi":"10.1002/mabi.202400415","DOIUrl":"https://doi.org/10.1002/mabi.202400415","url":null,"abstract":"Electrospun nanofibrous membranes made of chiral selectors (CSs) have shown their potential for efficient chiral resolutions via filtrations. It is thus of great importance to expand the number of electrospun membranes made of various CSs for the resolution of a wide range of chiral compounds. Here, the electrospinning of two benzyl carbamate derivatives of cellulose, namely cellulose benzyl carbamate (CBzC) and cellulose 4-chlorobenzyl carbamate (CCBzC), to form a new type of enantioselective membranes for chiral resolutions of racemic compounds, is reported. The morphology of the electrospun membranes is studied by optical microscopy and scanning electron microscopy in relation to the electrospinning process parameters. Liquid-liquid permeation experiments of the racemic compounds, (R,S)-1-(1-naphthyl)ethanol ((R,S)-NET), (R,S)-1,1'-bi-2-naphtol ((R,S)-BNP), (R,S)-naproxen ((R,S)-NAP), and (R,S)-benzoin ((R,S)-BNZ) through the membranes demonstrate preferable permeations of (R)- or (S)-enantiomers depending on the combinations between the CSs and the racemates. Molecular docking simulations indicate the differences in the binding type, number, and free energies between the CSs and the enantiomers.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2400415"},"PeriodicalIF":4.4,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0