Macromolecular bioscience最新文献_第4页

A Triple-Layered Composite Scaffold of Silk Fibroin and Decellularized Amniotic Membrane for Bladder Tissue Engineering. 用于膀胱组织工程的丝素-脱细胞羊膜三层复合支架。

IF 4.1 4区医学

Macromolecular bioscience Pub Date : 2025-08-14 DOI: 10.1002/mabi.202500157

Melina Mamdoohi, Mehdi Shafieian, Zahra Hassannejad

{"title":"A Triple-Layered Composite Scaffold of Silk Fibroin and Decellularized Amniotic Membrane for Bladder Tissue Engineering.","authors":"Melina Mamdoohi, Mehdi Shafieian, Zahra Hassannejad","doi":"10.1002/mabi.202500157","DOIUrl":"https://doi.org/10.1002/mabi.202500157","url":null,"abstract":"Augmentation cystoplasty has different side effects in urinary bladder reconstruction. Accordingly, it is necessary to develop substitutes using natural and synthetic biomaterials to address current problems. This study evaluates the potential of a triple-layered composite scaffold for bladder regeneration. The triple-layered scaffold consists of a silk fibroin (SF) film blended with polyethylene oxide (PEO), a decellularized human amniotic membrane (DHAM), and a lyophilized SF sponge, which is seeded with adipose tissue-derived stem cells (ADSCs) encapsulated in collagen hydrogel. The mechanical properties of the triple-layered scaffolds closely resemble those of human bladder tissue. The cell survival, proliferation, and viability of the different layers of the scaffold are assessed. The results show that DHAM and silk sponge at a concentration of 4% wt v-1 achieve a high level of biocompatibility. To study potential stone formation, scaffolds either with DHAM or without DHAM are exposed to human urine. Field emission scanning electron microscopy (FESEM) and X-ray diffraction analyses indicate that the scaffolds with DHAM do not exhibit any signs of erosion or the creation of crystalline particles after 7 days. In conclusion, the data presented in this study highlight a new triple-layered scaffold for the purpose of bladder tissue engineering.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00157"},"PeriodicalIF":4.1,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144847276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information: Macromol. Biosci. 8/2025 资料:宏mol。Biosci 8/2025。

IF 4.1 4区医学

Macromolecular bioscience Pub Date : 2025-08-14 DOI: 10.1002/mabi.202570016

引用次数: 0

Injectable Gellan Gum/Elastin-Based Nanocomposite Hydrogels as Filling Biomaterials for the Regeneration of Irregular Bone Defects. 结冷胶/弹性蛋白基纳米复合水凝胶在不规则骨缺损再生中的应用。

IF 4.1 4区医学

Macromolecular bioscience Pub Date : 2025-08-14 DOI: 10.1002/mabi.202500324

Giuseppe Barberi, Annalisa Martorana, Fabio Salvatore Palumbo, Cinzia Maria Chinnici, Giovanna Pitarresi, Calogero Fiorica

{"title":"Injectable Gellan Gum/Elastin-Based Nanocomposite Hydrogels as Filling Biomaterials for the Regeneration of Irregular Bone Defects.","authors":"Giuseppe Barberi, Annalisa Martorana, Fabio Salvatore Palumbo, Cinzia Maria Chinnici, Giovanna Pitarresi, Calogero Fiorica","doi":"10.1002/mabi.202500324","DOIUrl":"https://doi.org/10.1002/mabi.202500324","url":null,"abstract":"Irregular bone defects present a major challenge in clinical treatment. Traditional bone grafts are often used but come with limitations, including poor conformity to defect shapes and incomplete healing. Injectable hydrogels have emerged as a promising alternative, as they can completely fill defects and conform to irregular geometries. In this study, injectable hydrogels were developed using methacrylated gellan gum and soluble elastin derivatives. These polysaccharide/protein-based materials exhibit shear-thinning behavior and can be photo-crosslinked in situ, as confirmed by rheological analysis. The hydrogels are easily administered into irregular bone defects and solidified with light, resulting in improved stability and viscoelastic properties. Elastin derivatives enhance cell adhesion, supporting cell colonization. To further promote bone regeneration, ZnO and β-tricalcium phosphate (β-TCP) nanoparticles were incorporated into the hydrogel matrix. These bioactive fillers impart osteoconductive and osteoinductive properties without altering the mechanical integrity of the base material. Notably, β-TCP scaffolds modulated alkaline phosphatase activity in mesenchymal stromal cells, and ZnO further boosted this osteogenic marker. Overall, these injectable, photo-crosslinkable hydrogels offer a versatile platform for bone tissue engineering by functioning as defect fillers and bioactive scaffolds, supporting both structural and biological requirements for bone regeneration.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00324"},"PeriodicalIF":4.1,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144847277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Front Cover: Macromol. Biosci. 8/2025

IF 4.1 4区医学

Macromolecular bioscience Pub Date : 2025-08-14 DOI: 10.1002/mabi.202570015

引用次数: 0

Nitric Oxide Donor and Minoxidil Co-Loaded Microneedles Improving Hair Loss Treatment. 一氧化氮供体和米诺地尔共载微针改善脱发治疗。

IF 4.1 4区医学

Macromolecular bioscience Pub Date : 2025-08-06 DOI: 10.1002/mabi.202500225

Xueyang Wang, Xiaojie Ju, Chunyan He

{"title":"Nitric Oxide Donor and Minoxidil Co-Loaded Microneedles Improving Hair Loss Treatment.","authors":"Xueyang Wang, Xiaojie Ju, Chunyan He","doi":"10.1002/mabi.202500225","DOIUrl":"https://doi.org/10.1002/mabi.202500225","url":null,"abstract":"The local application of minoxidil tincture has been widely used clinically for the treatment of androgenetic alopecia (AGA). However, there are significant limitations, including ineffectiveness on its own, poor patient compliance, and low transdermal absorption. Microneedles (MNs) can puncture the stratum corneum painlessly and increase drug absorption through the skin. Additionally, nitric oxide (NO) has been shown to improve blood supply to hair follicles and reduce inflammation. Therefore, we prepared a kind of hair-growth-promoting MNs that can co-deliver minoxidil and NO donor for AGA treatment. In simple terms, nanogels (MHMA) were formed through the photopolymerization of methacrylate hyaluronic acid (MeHA) and methacrylate arginine (MeArg) as an NO donor. Through optimized formulation design, minoxidil was efficiently encapsulated within the MHMA nanogels with a particle size of 522.9 nm, which facilitates targeting of hair follicles. Subsequently, the MNs were prepared using a micro-molding method. Both in vitro and in vivo experiments demonstrated that the hair-growth-promoting MNs could efficiently deliver minoxidil and NO donor by just 3 min pressing on the mouse skin. It effectively promoted the transition of hair follicles to the growth phase and stimulated angiogenesis around the follicles, ultimately leading to hair regeneration in the alopecia mouse model. Overall, the proposed hair growth hair-growth-promoting MNs can efficiently and painlessly deliver minoxidil and NO donor into the skin, thus offering a promising new direction for clinical AGA treatment.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00225"},"PeriodicalIF":4.1,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel High-Efficient Method to Generate Fragmented Nano- and Microfibers Enabling an Additive for Bio-Inks. 一种新的高效方法生成破碎的纳米和微纤维，使生物墨水添加剂成为可能。

IF 4.1 4区医学

Macromolecular bioscience Pub Date : 2025-08-06 DOI: 10.1002/mabi.202500251

Margitta Büchner, Michael Geske, Michael Redel, Dirk W Schubert

{"title":"Novel High-Efficient Method to Generate Fragmented Nano- and Microfibers Enabling an Additive for Bio-Inks.","authors":"Margitta Büchner, Michael Geske, Michael Redel, Dirk W Schubert","doi":"10.1002/mabi.202500251","DOIUrl":"https://doi.org/10.1002/mabi.202500251","url":null,"abstract":"As an emerging technology, biofabrication combines biopolymers and living cells to create functional tissues, allowing the development of structures that closely mimic native tissues. The use of fiber-reinforced materials is of particular interest, as it enhances both mechanical properties and cellular behavior. Incorporating fiber fragments into bio-inks not only strengthens printed structures but also supports cell survival by lowering polymer concentrations and thus the stress exerted on the cells during printing. A key factor in optimizing fiber-reinforced bio-inks is the controlled fiber shortening, comprising cutting or breaking, which improves printability and mechanical integrity of printed constructs. However, current methods for fiber fragmentation face significant limitations, including material-specific dependencies, scalability challenges, and requirements of specialized equipment, which may not be accessible in all laboratories. To overcome these challenges, we introduce a novel approach utilizing ultraviolet irradiation to achieve controlled fiber fragmentation. The average fiber length resulting from specific irradiation times can be estimated using a multi-modal Weibull analysis. This technique is validated on fibers made of polycaprolactone (PCL) and gelatin blends, demonstrating its cost-effectiveness, biocompatibility, and simplicity. This study provides a practical solution for fiber fragment production and average length estimation, offering an accessible and scalable alternative for fiber-based biofabrication applications.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00251"},"PeriodicalIF":4.1,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis of Cellulose Hexanoate, Benzoate, and Mixed Esters: Exploring Their Potential as Enzyme Immobilization Platforms. 纤维素己酸酯、苯甲酸酯和混合酯的合成：探索它们作为酶固定平台的潜力。

IF 4.1 4区医学

Macromolecular bioscience Pub Date : 2025-08-06 DOI: 10.1002/mabi.202500221

Roberta Teixeira Polez, Thamiris Voltarelli Ferracini, Samuel Filipe Cardoso de Paula, Rachel Passos de Oliveira Santos, André L M Porto, Elisabete Frollini

{"title":"Synthesis of Cellulose Hexanoate, Benzoate, and Mixed Esters: Exploring Their Potential as Enzyme Immobilization Platforms.","authors":"Roberta Teixeira Polez, Thamiris Voltarelli Ferracini, Samuel Filipe Cardoso de Paula, Rachel Passos de Oliveira Santos, André L M Porto, Elisabete Frollini","doi":"10.1002/mabi.202500221","DOIUrl":"https://doi.org/10.1002/mabi.202500221","url":null,"abstract":"This study utilizes cellulose sourced from cotton linters to synthesize cellulose esters-hexanoate, benzoate, and mixed hexanoate-benzoate-with varying degrees of substitution (DS). These esters create electrospun mats that immobilize Pseudomonas fluorescens lipase (PFL), also in a configuration where an intermediate layer is added to a mat using an airbrush filled with PFL, covered by a third layer of electrospun mat. PFL-incorporated spheres are produced from cellulose ester solutions. DS, acyl chain length, and electrospinning parameters influence the morphology of the electrospun mat, which consists of nanofibers and ultrafine fibers. The PFL-incorporated mats show poor catalytic activity in resolving racemic (R,S)-2-chloro-1-phenylethanol, likely due to enzyme deactivation from high-voltage electrospinning. In contrast, mat-layered structures with PFL immobilized without voltage nearly doubled the conversion rate, although it was still lower than that of free enzymes. Spheres enhanced biocatalysis, achieving a 40% conversion rate with 94% enantiomeric purity while retaining 76% of their initial conversion rate in a subsequent reaction cycle. This research is the first to explore cellulose esters for the enzymatic immobilization of PFL to resolve a racemic mixture. The findings may enable PFL-incorporated structures in broader biocatalysis applications; the materials created may be tested to support the immobilization of other enzymes.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00221"},"PeriodicalIF":4.1,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peptide-Laden Silk Fibroin Microneedles Modulate Inflammation and Promote Myocardial Repair In Vitro. 载肽丝素微针体外调节炎症和促进心肌修复。

IF 4.1 4区医学

Macromolecular bioscience Pub Date : 2025-08-01 DOI: 10.1002/mabi.202400614

Xueting Wang, Jing Zhang, Jie Hui

引用次数: 0

Bacterial Nanocellulose-Chitosan-Gelatin-Hydroxyapatite Scaffolds for Bone Tissue Engineering. 细菌纳米纤维素-壳聚糖-明胶-羟基磷灰石骨组织工程支架。

IF 4.1 4区医学

Macromolecular bioscience Pub Date : 2025-07-31 DOI: 10.1002/mabi.202500299

Phasuwit P Phatchayawat, Supansa Yodmuang, Muenduen Phisalaphong

{"title":"Bacterial Nanocellulose-Chitosan-Gelatin-Hydroxyapatite Scaffolds for Bone Tissue Engineering.","authors":"Phasuwit P Phatchayawat, Supansa Yodmuang, Muenduen Phisalaphong","doi":"10.1002/mabi.202500299","DOIUrl":"https://doi.org/10.1002/mabi.202500299","url":null,"abstract":"Bacterial nanocellulose (BNC) is highly biocompatible and has excellent mechanical properties, but lacks bioactive properties. In this study, scaffolds of BNC composites with suitable physical and biological properties for bone tissue regeneration were successfully fabricated through a simple, facile, cost-effective, and scalable method via biosynthesis by Acetobacter xylinum in a culture medium supplemented with chitosan (CS), gelatin (GT), and hydroxyapatite (HAp). BNC-CS-GT-HAp scaffolds displayed a good 3D architecture of interconnected porous structures with fiber networks and improved surface roughness upon HAp incorporation, with pore diameters of 384.5-457.4 µm on the surface and 467.5-498.7 µm in the interior, along with porosity of 66.0%-81.4%. Adding HAp to scaffolds at 0.1% to 0.2% (w/v) improved scaffold properties, such as compressive strength (MPa), thermal stability, and antibacterial properties. BNC-CS-GT-HAp scaffolds were biomineralized in a simulated body fluid for 21 days, producing bone-like apatite with a Ca/P ratio of 1.65-1.69. The in vitro study of MC3T3-E1 cells showed that BNC-CS-GT-HAp scaffolds facilitated cell adsorption, adhesion, and proliferation. They also promoted alkaline phosphatase (ALP) activity and extracellular matrix (ECM) mineralization. On day 21, the cell-seeded scaffolds showed significantly improved compressive strength compared to cell-free scaffolds. The results of this study suggest that BNC-CS-GT-HAp scaffolds could enhance osteoconductivity, possess desirable properties for bone tissue engineering, and provide a promising platform for future translational and in vivo studies.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00299"},"PeriodicalIF":4.1,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144753737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D Bioprintable Gt-Alg-MMT Nano Bioink for Cartilage Tissue Engineering. 用于软骨组织工程的3D生物打印Gt-Alg-MMT纳米生物链接。

IF 4.1 4区医学

Macromolecular bioscience Pub Date : 2025-07-31 DOI: 10.1002/mabi.202500167

Xiaofang Wu, Kai Chen, Qin Chen, Xinyue Zhang, Cunao Feng, Xiaowei Li, Dekun Zhang

{"title":"3D Bioprintable Gt-Alg-MMT Nano Bioink for Cartilage Tissue Engineering.","authors":"Xiaofang Wu, Kai Chen, Qin Chen, Xinyue Zhang, Cunao Feng, Xiaowei Li, Dekun Zhang","doi":"10.1002/mabi.202500167","DOIUrl":"https://doi.org/10.1002/mabi.202500167","url":null,"abstract":"Damage to articular cartilage is irreversible, and its self-healing ability is minimal. The construction of articular cartilage in tissue engineering requires suitable biomaterials as scaffolds to provide a 3D natural microenvironment for the development and growth of articular cartilage. This study aims to explore the feasibility of Gt-Alg-MMT (gelatin/sodium alginate/montmorillonite) nanocomposite hydrogel as a 3D printing bioink and its applicability in 3D printing cartilage scaffolds. The optimization results showed that the bioink with the ratio of 2Gt-5Alg-5MMT had the best 3D printability and mechanical strength, and the optimal 3D printing pressure and printing speed were 0.29 MPa and 2.5 mm/s, respectively. The performance test showed that the 3D printed 2Gt-5Alg-5MMT scaffold has a honeycomb porous network structure, with porosity and water content of more than 90%, static compressive elastic modulus of 125 ± 9.6 kPa, hysteresis of cyclic compression of 60%-80%, and has viscoelasticity, structural stability, and thermal stability close to cartilage tissue in three scanning modes of dynamic strain, dynamic frequency, and dynamic temperature. In addition, Live/Dead staining experiments showed that the 2Gt-5Alg-5MMT scaffold has excellent biocompatibility with ADTC5 cells. Therefore, this 3D-printed 2Gt-5Alg-5MMT scaffold is expected to be a candidate material for promoting articular cartilage regeneration.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e00167"},"PeriodicalIF":4.1,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144760487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0