Macromolecular bioscience最新文献_第4页

Combinatorial Synthesis of Alkyl Chain-Capped Poly(β-Amino Ester)s for Effective siRNA Delivery. 用于有效递送 siRNA 的烷基链封端聚（β-氨基酯）的组合合成。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-07-25 DOI: 10.1002/mabi.202400168

Baiqiu Chen, Qidi Ren, Pingge Jiang, Qiong Wu, Qi Shuai, Yunfeng Yan

{"title":"Combinatorial Synthesis of Alkyl Chain-Capped Poly(β-Amino Ester)s for Effective siRNA Delivery.","authors":"Baiqiu Chen, Qidi Ren, Pingge Jiang, Qiong Wu, Qi Shuai, Yunfeng Yan","doi":"10.1002/mabi.202400168","DOIUrl":"https://doi.org/10.1002/mabi.202400168","url":null,"abstract":"Poly (β-amino ester) (PBAE) is a class of biodegradable polymers containing ester bonds in their main chain, extensively investigated as cationic polymer carriers for siRNA. Most current PBAE carriers rely on termination with hydrophilic or charged amines. In this study, a polymer platform consisting of 168 PBAE polymers with hydrophobic alkyl chain terminals is constructed through sequential aza-Michael addition. A large number of effective carriers are identified through in vitro screening of the PBAE platform for siLuc delivery to HeLa-Luc cells. Specifically, PA8-C6 and PA8-C8 achieve remarkable gene knockdown efficacies of up to 80% with low cytotoxicity. Certain materials from the PA2 and PA5 series demonstrate potent siRNA delivery capabilities associated with elevated cytotoxicity. The pKa value of PBAE is predominantly determined by the hydrophilic amine side chains rather than the end-capping groups. A pKa range of ≈6.2-6.5 may contribute to the excellent delivery capability for PA8 series carriers. The co-formulation of PBAE carriers with helper lipids leads to the reduced size and surface charges of the polyplex NPs with siRNA, consequently decreasing the cytotoxicity and enhancing siRNA delivery efficacy. These findings hold significant implications for the development of novel degradable polymer carriers for siRNA delivery.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141759637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the Mechanical Strength and Cell Adhesion Capacity of POSS Doped PVA/CMC Hernia Patch. 评估掺杂 POSS 的 PVA/CMC Hernia 修补片的机械强度和细胞粘附能力。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-07-25 DOI: 10.1002/mabi.202400095

Meryem Akkurt Yıldırım, Barkın Özer, Nelisa Türkoğlu, Cenk Denktaş

{"title":"Evaluation of the Mechanical Strength and Cell Adhesion Capacity of POSS Doped PVA/CMC Hernia Patch.","authors":"Meryem Akkurt Yıldırım, Barkın Özer, Nelisa Türkoğlu, Cenk Denktaş","doi":"10.1002/mabi.202400095","DOIUrl":"https://doi.org/10.1002/mabi.202400095","url":null,"abstract":"Peritoneal adhesion typically occurs in applications such as abdominal, pelvic, and vascular surgery. It is necessary to develop a mechanical barrier to prevent adhesion. In this study, a novel biomaterial as a mechanical barrier is developed by combining polyvinyl alcohol (PVA) and carboxymethyl cellulose (CMC), doped with polyhedral oligomeric silsesquioxane (POSS) to prevent peritoneal adhesion. Fourier-transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) methods reveal that POSS nanoparticles in the PVA matrix disrupted the intramolecular hydroxyl groups and structure of the crystal region. Electron microscopy (EM) images reveal that high concentrations of POSS (2 wt.%) cause irregular clustering in the composite matrix. As the concentration of POSS increases in the matrix, the degradation of the membranes increases, and protein adhesion decreases. In vitro cytotoxicity tests show a toxic effect on cells for PVA/CMC composite membranes, while on the other hand, the addition of POSS increases cell viability. According to the MMT test the POSS decreases cell adhesion of membranes. When comparing the POSS doped membrane to the undoped PVA/CMC membrane, an increase in the total antioxidant level and a decrease in the total oxidant level is observed.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141759638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Boosting the Cell Harvesting Performance of Poly(di(ethylene glycol)methyl ether methacrylate) Cell Release Layers via Copolymerization of Photo- and Thermoresponsive Monomers. 通过光致和热致单体的共聚提高聚(二(乙二醇)甲基醚甲基丙烯酸酯)细胞释放层的细胞收集性能。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-07-25 DOI: 10.1002/mabi.202400249

Siyu Jiang, Sijia Yang, Wenwei Lei, Zhiwei Liu, Holger Schönherr

{"title":"Boosting the Cell Harvesting Performance of Poly(di(ethylene glycol)methyl ether methacrylate) Cell Release Layers via Copolymerization of Photo- and Thermoresponsive Monomers.","authors":"Siyu Jiang, Sijia Yang, Wenwei Lei, Zhiwei Liu, Holger Schönherr","doi":"10.1002/mabi.202400249","DOIUrl":"https://doi.org/10.1002/mabi.202400249","url":null,"abstract":"The performance of the cell-selective thermoresponsive poly(di(ethylene glycol)methyl ether methacrylate) (PDEGMA) cell harvest system is shown to be drastically enhanced by exploiting the combination of photoresponsive spiropyran derivates and PDEGMA in copolymerized brushes. The analysis of copolymerized 1'-(2-methacryloxyethyl)-3',3'-dimethyl-6-nitrospiro(2H-1-benzopyran-2,2'-indoline) (SPMA) (DEMGA) di(ethylene glycol)methyl ether methacrylate brushes revealed that a minor adjustment of the SPMA/DEGMA ratios results in a significant alternation of wettability as well as protein adsorption, when switching the temperature from 37 to 22 °C and alternately irradiating using different light wavelengths (from 530 to 365 nm). Thin P(SPMA-co-DEGMA) layers supported pancreatic tumor PaTu 8988t cells with high cell viability. Copolymer layers with 2.5% SPMA/DEGMA led to the highest efficiency of enzyme-free cell release with very good cell viability. The release is induced by cooling the cell culture medium to 22 °C and irradiating the surface with 365 nm light. Compared to neat PDEGMA, the P(SPMA-co-DEGMA) layers showed a threefold increase in the speed of the change of cell morphology of the attached cells and a >5 times increased fraction of detached cells, which underlines the potential of these dual responsive PDEGMA systems for optimized performance in the facile capture, culture, and release of different cell lines.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141759636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of Manipulative In Situ Pore-Formation upon Polymeric Coating on Cylindrical Substrate for Sustained Drug Delivery. 研究在圆柱形基底上涂覆聚合物后原位形成孔隙，以实现持续给药。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-07-22 DOI: 10.1002/mabi.202400273

Hao Wei, Ping Lin, Baozhang Shi, Liping Xu, Xiaoping Yang, Wei Sun

{"title":"Study of Manipulative In Situ Pore-Formation upon Polymeric Coating on Cylindrical Substrate for Sustained Drug Delivery.","authors":"Hao Wei, Ping Lin, Baozhang Shi, Liping Xu, Xiaoping Yang, Wei Sun","doi":"10.1002/mabi.202400273","DOIUrl":"https://doi.org/10.1002/mabi.202400273","url":null,"abstract":"Herein, the micro-porous polylactic acid coating applied on the surface of the cylindrical substrate is fabricated by a novel in situ pore-formation strategy based on the combinational effect of breath figure (BF) and vapor-induced phase separation (VIPS) processes. Under the condition of high environmental humidity, solvent pair of chloroform and dimethylformamide is employed for post-treatment onto pre-formed PLA coating to induce the pore-formation following the mechanism of BF and VIPS, respectively. A composite porous structure with both cellular-like and bi-continuous network morphologies is obtained. By tunning the experimental factors including the ratio of the solvent pair, environmental humidity, and temperature, morphological manipulation upon the pore morphology can be facilely achieved based on the control of mechanism transition between BF and VIPS. Paclitaxel is used as a model drug and loaded into the porous coating by the wicking effect of post-immersion. Coatings with different morphological features show varying drug loading and release capacities. The 28-day release test reveals dynamic release profiles between different coating samples, with the total release rate ranging from 35.70% to 79.96%. Optimal loading capacity of 19.28 µg cm-2 and 28-day release rate of 35.70% are achieved for the coating with composite BF-VIPS structure. This research established a cost-efficient strategy with high flexibility in the structural manipulation concerning the construction of drug-eluting coating with the feature of manipulative drug delivery.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141748528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking the Potential: PEGylation and Molecular Weight Reduction of Ionenes for Enhanced Antifungal Activity and Biocompatibility 释放潜力：离子烯的 PEG 化和分子量降低可增强抗真菌活性和生物相容性。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-07-17 DOI: 10.1002/mabi.202400032

Jan M. Kurzyna, Rafał J. Kopiasz, Martyna Paul, Magdalena Flont, Patrycja Baranowska, Jolanta Mierzejewska, Karolina Drężek, Waldemar Tomaszewski, Elżbieta Jastrzębska, Dominik Jańczewski

{"title":"Unlocking the Potential: PEGylation and Molecular Weight Reduction of Ionenes for Enhanced Antifungal Activity and Biocompatibility","authors":"Jan M. Kurzyna, Rafał J. Kopiasz, Martyna Paul, Magdalena Flont, Patrycja Baranowska, Jolanta Mierzejewska, Karolina Drężek, Waldemar Tomaszewski, Elżbieta Jastrzębska, Dominik Jańczewski","doi":"10.1002/mabi.202400032","DOIUrl":"10.1002/mabi.202400032","url":null,"abstract":"Numerous synthetic polymers, imitating natural antimicrobial peptides, have demonstrated potent antimicrobial activity, positioning them as potential candidates for new antimicrobial drugs. However, the high activity of these molecules often comes at the cost of elevated toxicity against eukaryotic organisms. In this study, a series of cationic ionenes with varying molecular weights to assess the influence of polymer chain length on ionene activity is investigated. To enhance polymer antimicrobial activity and limit toxicity a PEG side chain is introduced into the repeating unit. The resulting molecules consistently exhibited high activity against three model organisms: E. coli, S. aureus and C. albicans. The incorporation of side PEG chain improves antifungal properties and biocompatibility, regardless of molecular weight. The most important finding of this work is that the reduction of polymer molecular mass led to increased antifungal activity and reduced cytotoxicity against HMF and MRC-5 cell lines simultaneously. As a result, the best-performing molecules reported herein displayed minimal inhibitory concentrations (MIC) as low as 2 and 0.0625 µg mL1 for C. albicans and C. tropicalis respectively, demonstrating exceptional selectivity. It is plausible that some of described herein molecules can serve as potential lead candidates for new antifungal drugs.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141633908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitrilotriacetic Acid Functionalized Microgels for Efficient Immobilization of Hyaluronan Synthase 用于高效固定透明质酸合成酶的 Nitrilotriacetic Acid Functionalized Microgels。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-07-17 DOI: 10.1002/mabi.202400075

Isabel Katja Sommerfeld, Esther Maria Dälken, Lothar Elling, Andrij Pich

{"title":"Nitrilotriacetic Acid Functionalized Microgels for Efficient Immobilization of Hyaluronan Synthase","authors":"Isabel Katja Sommerfeld, Esther Maria Dälken, Lothar Elling, Andrij Pich","doi":"10.1002/mabi.202400075","DOIUrl":"10.1002/mabi.202400075","url":null,"abstract":"Enzymes play a vital role in synthesizing complex biological molecules like hyaluronic acid (HA). Immobilizing enzymes on support materials is essential for their efficient use and reuse in multiple cycles. Microgels, composed of cross–linked, highly swollen polymer networks, are ideal for enzyme uptake owing to their high porosity. This study demonstrates the immobilization of His6-tagged hyaluronan synthase from Pasteurella multocida (PmHAS) onto nitrilotriacetic acid functionalized microgels using different bivalent ions (Ni2+, Co2+, Mn2+, Mg2+, and Fe2+) via metal affinity binding. The results indicate that using Ni2+ yields the microgels with the highest enzyme uptake and HA formation. The immobilized PmHAS enables repetitive enzymatic production, producing high molecular weight HAs with decreasing dispersities in each step. Furthermore, the highest reported yield of HA with high molecular weight for immobilized PmHAS is achieved. This system establishes a foundation for continuous HA formation, with future works potentially enhancing PmHAS stability through protein engineering.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202400075","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141633907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vancomycin-Loaded Sol–Gel System for In Situ Coating of Artificial Bone to Prevent Surgical Site Infections 用于人工骨原位涂层的万古霉素载体溶胶凝胶系统，可预防手术部位感染。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-07-16 DOI: 10.1002/mabi.202400078

Xin Cui, Tian-Ci Wei, Lu-Ming Guo, Guo-Yang Xu, Kuo Zhang, Qing-Shi Zhang, Xiong Xu, Gui-Yuan Wang, Litao Li, Hong-Wen Liang, Lei Wang, Xu Cui

{"title":"Vancomycin-Loaded Sol–Gel System for In Situ Coating of Artificial Bone to Prevent Surgical Site Infections","authors":"Xin Cui, Tian-Ci Wei, Lu-Ming Guo, Guo-Yang Xu, Kuo Zhang, Qing-Shi Zhang, Xiong Xu, Gui-Yuan Wang, Litao Li, Hong-Wen Liang, Lei Wang, Xu Cui","doi":"10.1002/mabi.202400078","DOIUrl":"10.1002/mabi.202400078","url":null,"abstract":"Surgical site infections (SSIs) related to implants have always been a major challenge for clinical doctors and patients. Clinically, doctors may directly apply antibiotics into the wound to prevent SSIs. However, this strategy is strongly associated with experience of doctors on the amount and the location of antibiotics. Herein, an in situ constructable sol–gel system is developed containing antibiotics during surgical process and validated the efficacy against SSIs in beagles. The system involves chitosan (CS), β-glycerophosphate (β-GP) and vancomycin (VAN), which can be adsorbed onto porous hydroxyapatite (HA) and form VAN-CS/β-GP@HA hydrogel in a short time. The VAN concentration from VAN-CS/β-GP@HA hydrogel is higher than minimum inhibitory concentration (MIC) against Staphylococcus aureus (S. aureus) at the 21st day in vitro. In an in vivo canine model for the prevention of SSIs in the femoral condyle, VAN-CS/β-GP@HA exhibits excellent biocompatibility, antimicrobial properties, and promotion of bone healing. In all, the CS/β-GP instant sol–gel system is able to in situ encapsulate antibiotics and adhere on artificial bone implants during the surgery, effectively preventing SSIs related to implants.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141620308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Masthead: Macromol. Biosci. 7/2024 刊头：Macromol.Biosci.7/2024

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-07-15 DOI: 10.1002/mabi.202470017

引用次数: 0

Optimization of Zwitterionic Polymers for Cell Cryopreservation 优化用于细胞冷冻保存的聚合离子聚合物

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-07-15 DOI: 10.1002/mabi.202470016

Yui Kato, Takuya Uto, Takeru Ishizaki, Daisuke Tanaka, Kojiro Ishibashi, Yuya Matsuda, Issei Onoda, Akiko Kobayashi, Masaharu Hazawa, Richard W. Wong, Kenji Takahashi, Eishu Hirata, Kosuke Kuroda

引用次数: 0

Challenges and Opportunities in Developing Tracheal Substitutes for the Recovery of Long-Segment Defects 开发用于恢复长段缺损的气管替代物的挑战与机遇。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-07-15 DOI: 10.1002/mabi.202400054

Kausik Kapat, Prashil Gondane, Sakshi Kumbhakarn, Shruti Takle, Rahul Sable

{"title":"Challenges and Opportunities in Developing Tracheal Substitutes for the Recovery of Long-Segment Defects","authors":"Kausik Kapat, Prashil Gondane, Sakshi Kumbhakarn, Shruti Takle, Rahul Sable","doi":"10.1002/mabi.202400054","DOIUrl":"10.1002/mabi.202400054","url":null,"abstract":"Tracheal resection and reconstruction procedures are necessary when stenosis, tracheomalacia, tumors, vascular lesions, or tracheal injury cause a tracheal blockage. Replacement with a tracheal substitute is often recommended when the trauma exceeds 50% of the total length of the trachea in adults and 30% in children. Recently, tissue engineering and other advanced techniques have shown promise in fabricating biocompatible tracheal substitutes with physical, morphological, biomechanical, and biological characteristics similar to native trachea. Different polymers and biometals are explored. Even with limited success with tissue-engineered grafts in clinical settings, complete healing of tracheal defects remains a substantial challenge due to low mechanical strength and durability of the graft materials, inadequate re-epithelialization and vascularization, and restenosis. This review has covered a range of reconstructive and regenerative techniques, design criteria, the use of bioprostheses and synthetic grafts for the recovery of tracheal defects, as well as the traditional and cutting-edge methods of their fabrication, surface modification for increased immuno- or biocompatibility, and associated challenges.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141620307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0