Macromolecular bioscience最新文献_第4页

Evaluation of Electrospun Poly-4-Hydroxybutyrate as Biofunctional and Degradable Scaffold for Pelvic Organ Prolapse in a Vaginal Sheep Model 静电纺聚4-羟基丁酸酯作为阴道羊盆腔器官脱垂生物功能和可降解支架的评价。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-02-26 DOI: 10.1002/mabi.202400412

Krista L. C. van Rest, Stephen T. Jeffrey, Lisa Kaestner, Aksel Gudde, Anel Oosthuysen, Jan-Paul W. R. Roovers, Zeliha Guler

{"title":"Evaluation of Electrospun Poly-4-Hydroxybutyrate as Biofunctional and Degradable Scaffold for Pelvic Organ Prolapse in a Vaginal Sheep Model","authors":"Krista L. C. van Rest, Stephen T. Jeffrey, Lisa Kaestner, Aksel Gudde, Anel Oosthuysen, Jan-Paul W. R. Roovers, Zeliha Guler","doi":"10.1002/mabi.202400412","DOIUrl":"10.1002/mabi.202400412","url":null,"abstract":"Pelvic organ prolapse (POP) affects many women, especially after menopause. POP occurs due to the descent of weakened supportive tissue. Current prolapse surgeries have high failure rates, due to disturbed wound healing caused by lower tissue regeneration and estrogen depletion. Absorbable poly-4-hydroxybutyrate (P4HB) knit implants exhibited improved cell and tissue response leading to less complications from prolapse surgery. This study aims to enhance wound healing and improve surgical outcomes by using an electrospun (ES) P4HB scaffold (ES P4HB) that emulates natural tissue structure. Further 17β-estradiol (E2)-a prominent wound healing factor-is incorporated into the scaffold (ES P4HB-E2). Parous Dohne Merino sheep underwent posterior vaginal wall implantation of either P4HB (n = 6) or 17β-estradiol relasing P4HB-E2 (n = 6) scaffolds, or underwent native tissue repair (NTR) (n = 4). Vaginal explants were compared for short-term host response in terms of gross necropsy, histomorphology, biomechanics, tissue-integration, and degradation of P4HB at 3-months post-implantation. Both scaffolds show promising results with enhanced mechanical properties and increased macrophage infiltration compared to NTR, but without differences between scaffolds. Thus, it seems electrospun P4HB scaffolds already improve tissue integration and healing. Further long-term studies are needed before these scaffolds can be used in clinical practice.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"25 4","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202400412","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143501988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adjusting Morphology, Structure, and Mechanical Properties of Electrospun High-Molecular-Weight Poly(l-Lactic-Acid) Nanofibrous Yarns Through Hot Stretching Treatment. 热拉伸处理对静电纺丝高分子量聚乳酸纳米纤维纱线形态、结构和力学性能的调节。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-02-22 DOI: 10.1002/mabi.202400656

Xiaoyu Liu, Jiayi Jiang, Hailei Liu, Fei Liu, Huarong Shao, Shaojuan Chen, Shaohua Wu

{"title":"Adjusting Morphology, Structure, and Mechanical Properties of Electrospun High-Molecular-Weight Poly(l-Lactic-Acid) Nanofibrous Yarns Through Hot Stretching Treatment.","authors":"Xiaoyu Liu, Jiayi Jiang, Hailei Liu, Fei Liu, Huarong Shao, Shaojuan Chen, Shaohua Wu","doi":"10.1002/mabi.202400656","DOIUrl":"https://doi.org/10.1002/mabi.202400656","url":null,"abstract":"An integrated strategy that combines innovative electrospinning technique with traditional hot-stretching post-treatment is designed and implemented to generate high-molecular-weight poly(l-lactic-acid) (hmwPLLA, Mw = 2 80 000 Da) electrospun nanofiber-constructed yarns (ENCYs). The internal fiber diameter within the hmwPLLA ENCYs is found to increase gradually with the increase of hmwPLLA solution concentration. The hmwPLLA ENCY generated from a concentration of 10% (w v-1) is demonstrated with uniform morphology with an average fiber diameter of 737.7 ± 72.2 nm and an average yarn diameter of 454.9 ± 3.5 µm. Compared with the unstretched hmwPLLA ENCY, increasing the hot-stretching temperature can significantly enhance the fiber orientation and crystallinity. Moreover, the mechanical properties of stretched ENCYs are obviously enhanced compared with the unstretched control. The fiber orientation and crystallinity of stretched ENCYs are also found to be significantly improved with the increase of hot stretching rate, further resulting in the obvious increase of breaking strength and Young's modulus. Importantly, the braided textiles made from stretched hmwPLLA ENCYs exhibited great biocompatibility by effectively guiding the cell alignment and supporting the cell adhesion and proliferation. In summary, the high performance hmwPLLA ENCYs show great potential for the future design and development of advanced biomedical textiles.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2400656"},"PeriodicalIF":4.4,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143476824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation and Evaluation of RGD-Conjugated Crosslinked PVA Tissue Engineered Vascular Scaffold with Endothelial Differentiation and Its Impact on Vascular Regeneration In Vivo. rgd共轭交联PVA组织工程内皮分化血管支架的制备、评价及其对血管再生的影响。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-02-22 DOI: 10.1002/mabi.202400554

Xiafei Li, Xuewei Zhang, Yameng Wang, Shenglu Ji, Ziwei Zhao, Jianshen Yin, Tuo Yang, Xin Feng, Hongli Chen, Wenbin Li, Xianwei Wang, Changqin Jing, Dan Ding, Liang Zhao

{"title":"Preparation and Evaluation of RGD-Conjugated Crosslinked PVA Tissue Engineered Vascular Scaffold with Endothelial Differentiation and Its Impact on Vascular Regeneration In Vivo.","authors":"Xiafei Li, Xuewei Zhang, Yameng Wang, Shenglu Ji, Ziwei Zhao, Jianshen Yin, Tuo Yang, Xin Feng, Hongli Chen, Wenbin Li, Xianwei Wang, Changqin Jing, Dan Ding, Liang Zhao","doi":"10.1002/mabi.202400554","DOIUrl":"https://doi.org/10.1002/mabi.202400554","url":null,"abstract":"PVA has emerged as a prevalent material for the construction of vascular tissue engineering scaffolds. Nonetheless, the integration of 3D crosslinked polyvinyl alcohol (PVA) scaffolds featuring arginine-glycine-aspartate (RGD) binding remains a rarity in tissue engineering. In the present study, a PVA-4-azidobenzoic acid (AZ)-RGD scaffold is prepared based on cross-linking of two distinct PVA derivatives: one featuring photoreactive azides for ultraviolet (UV)-crosslinking and the other incorporating RGD peptides. The results show that the PVA-AZ-RGD scaffold has good blood compatibility and biomechanical properties, with hydrophilic properties, and a hydrolysis rate of 27.31% at 12 weeks. Notably, the incorporation of RGD peptides significantly bolsters the attachment and proliferation of mesenchymal stem cells (MSCs) on the scaffolds, compared to non-RGD-conjugated controls. Furthermore, RGD conjugation markedly accelerates endothelialization of MSCs following 15 days of endothelial culture. Post-transplantation, the PVA-AZ-RGD scaffold exhibits favorable blood flow patency, minimal immune rejection, promotes endothelialization and smooth muscle cell proliferation, and facilitates the development of extracellular matrix, ultimately contributing to the formation of regenerative artificial blood vessels. These comprehensive findings underscore the promising potential of RGD-integrated, crosslinked PVA scaffolds for applications in vascular tissue engineering.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2400554"},"PeriodicalIF":4.4,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143476834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Melanin-Inspired Maleimide Coatings on Various Substrates for Rapid Thiol Functionalization. 黑色素激发马来酰亚胺涂层在各种基底上的快速硫醇功能化。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-02-20 DOI: 10.1002/mabi.202400616

Suho Park, Himani Bisht, Seongchul Park, Jaehoon Jeong, Yubin Hong, Daeun Chu, Minseob Koh, Daewha Hong

{"title":"Melanin-Inspired Maleimide Coatings on Various Substrates for Rapid Thiol Functionalization.","authors":"Suho Park, Himani Bisht, Seongchul Park, Jaehoon Jeong, Yubin Hong, Daeun Chu, Minseob Koh, Daewha Hong","doi":"10.1002/mabi.202400616","DOIUrl":"https://doi.org/10.1002/mabi.202400616","url":null,"abstract":"In this study, a substrate-independent maleimide film is developed that can be formed under mild aqueous conditions (pH 7.4), and which allows rapid and efficient external thiol immobilization onto the coated surfaces. For the coating block, tyrosine-conjugated maleimide (Tyr-Mal) containing a phenolic amine moiety is prepared as a substrate-independent dormant coating precursor, wherein the maleimide component permits a rapid Michael addition reaction with the thiol moiety of interest. By mimicking natural melanogenesis, Tyr-Mal acts as a substrate for tyrosinase under physiological conditions (pH 7.4) to form a melanin-inspired maleimide (Mel-Mal) film on various substrates, including living cell surfaces. The resulting film undergoes a rapid surface reaction (< 30 min) with external thiol groups under mild aqueous conditions. Considering that a typical polydopamine film requires a long reaction time (≈3 h) under alkaline conditions (pH 8.5) to achieve thiol functionalization with low efficiency, the current surface platform demonstrates significant improvements in terms of its reaction kinetics and usability. Moreover, considering that thiol functionalization and surface coating are performed under mild aqueous conditions, it is expected that the developed Mel-Mal film will be a useful tool in the fields of cell surface engineering, microarrays, and high-throughput screening.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":" ","pages":"e2400616"},"PeriodicalIF":4.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143458562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Vitro Evaluation of a Semi-Autologous Fibrin Sealant for Surgical Applications 半自体纤维蛋白密封胶在外科应用中的体外评价。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-02-20 DOI: 10.1002/mabi.202400165

Uri Elbaz, Ori Berliner, Shavit Tabo, Shani Yeshayahu, Reut Kesner, Dana Cohen-Gerassi, Lihi Adler-Abramovich, Michal Halperin-Sternfeld, Moran Aviv

{"title":"In Vitro Evaluation of a Semi-Autologous Fibrin Sealant for Surgical Applications","authors":"Uri Elbaz, Ori Berliner, Shavit Tabo, Shani Yeshayahu, Reut Kesner, Dana Cohen-Gerassi, Lihi Adler-Abramovich, Michal Halperin-Sternfeld, Moran Aviv","doi":"10.1002/mabi.202400165","DOIUrl":"10.1002/mabi.202400165","url":null,"abstract":"Surgical success relies on precise tissue approximation using sutures, clips, or staples. Fibrin sealant provides a user-friendly alternative, saving time and maintaining tissue integrity. Yet, its cost and potential bioburden risk are notable drawbacks. To address these concerns, a semi-autologous fibrin sealant is produced from human cryoprecipitate and compared it to a commercial fibrin sealant. The microstructure of the semi-autologous sealant closely resembles the commercial one. Initially, the commercial sealant has superior bonding strength, however, over time, both demonstrate strong adhesive properties. Moreover, when the two sealants contain equivalent fibrinogen concentrations, they show similar bonding strength and rheological properties, including thixotropic behavior, which is essential for their application as bioadhesives. Notably, it is discovered that the mechanical properties of the adhesive are mainly governed by the fibrinogen concentration, with minimal impact of other blood components. This understanding paves the way for the development of an efficient method to boost fibrinogen in blood without extensive separation. This study indicates semi-autologous fibrin glue matches commercial sealant in adhesive properties. This may offer several advantages, such as reduced bioburden, costs, improved immunomodulation, and reduced hypersensitivity and virus transmission risks. These findings hold promising prospects for enhancing the wound healing process in various medical conditions.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"25 4","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202400165","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143458561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information: Macromol. Biosci. 2/2025 资料:宏mol。Biosci 2/2025。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-02-14 DOI: 10.1002/mabi.202570004

引用次数: 0

A Novel Crosslinking Approach for Biomanufacturing of a Collagen-Based Skin Dermal Template 一种新的交联方法用于胶原基皮肤模板的生物制造

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-02-14 DOI: 10.1002/mabi.202570003

Weng Wan Chan, Keya Rani Roy, Bach Quang Le, Hariharan Ezhilarasu, Xiaoqian Zhang, Ryan Yi Da Lim, Avinanda Banerjee, Mariya Kuriakose, Krystle Joy Ng, Priya Murugan, Chun Ting Goh, Weibiao Zhou, May Win Naing, Srikala Raghavan, Deepak Choudhury

引用次数: 0

A Silibinin-Poly(ε-Caprolactone) Conjugate as an Enhanced Anticancer Agent 水飞蓟宾-聚（ε-己内酯）偶联物的增强型抗癌剂。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-02-12 DOI: 10.1002/mabi.202400510

Fabiana Vento, Anna Privitera, Giuseppe Caruso, Angelo Nicosia

{"title":"A Silibinin-Poly(ε-Caprolactone) Conjugate as an Enhanced Anticancer Agent","authors":"Fabiana Vento, Anna Privitera, Giuseppe Caruso, Angelo Nicosia","doi":"10.1002/mabi.202400510","DOIUrl":"10.1002/mabi.202400510","url":null,"abstract":"Poly(ε-caprolactone) (PCL) is a hydrolytically degradable biopolyester used in drug delivery to enhance drug solubility and bioavailability, where drugs are typically incorporated physically within the biopolymeric matrix rather than covalently bonded, due to the limited availability of functional groups required for covalent attachment. In pursuit of developing a facile method for the production of a biopolyester-drug covalent conjugate with effective drug loading capacity, this study reports the synthesis of a covalent Silibinin-PCL conjugate (Sil-PCLHyd) through a two-step approach. This involves the controlled hydrolysis of a high molecular weight PCL to increase the concentration of carboxylic end groups, which are subsequently used for the catalyzed esterification with Silibinin. The Sil-PCLHyd is characterized with mass spectrometry, gel permeation chromatography, thermogravimetric analysis, differential scanning calorimetry, and NMR and UV–vis spectroscopies. The cytotoxic effects of Sil-PCLHyd against colorectal adenocarcinoma cells (Caco-2) are measured through the MTT assay. The results of the Sil-PCLHyd characterization revealed a Silibinin loading of ≈9.8 wt.%. The MTT assay demonstrated that Sil-PCLHyd induced cytotoxic effects at concentrations a hundred times lower than those required for free Silibinin. The proposed approach might represent a reliable pathway for the development of biopolyester-based covalent conjugates with a high drug loading capacity.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"25 4","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202400510","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro Bone Tissue Engineering Strategies: The Relevance of Cells and Culturing Methods in Bone Formation and Remodeling 体外骨组织工程策略：细胞和培养方法在骨形成和重塑中的相关性。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-02-11 DOI: 10.1002/mabi.202400453

Ana Raquel Bastos, Fátima Raquel Maia, Joaquim Miguel Oliveira, Rui Luís Reis, Vítor Manuel Correlo

{"title":"In vitro Bone Tissue Engineering Strategies: The Relevance of Cells and Culturing Methods in Bone Formation and Remodeling","authors":"Ana Raquel Bastos, Fátima Raquel Maia, Joaquim Miguel Oliveira, Rui Luís Reis, Vítor Manuel Correlo","doi":"10.1002/mabi.202400453","DOIUrl":"10.1002/mabi.202400453","url":null,"abstract":"The most recent advances in bone tissue engineering (BTE) approaches step forward in the field of three-dimensional (3D) tissue models, enabling the development of more realistic tools to study bone disorders, such as osteoporosis. BTE field aims to mimic native bone tissue more truthfully, providing an appropriate environment for tissue regeneration and repair through the combination of 3D porous scaffolds, specific growth factors, and cells. Currently, the scientific community is focused on developing and improving new biomaterials that in combination with growth factors and specific cell types, that can accurately emulate the native bone microenvironment. However, most of the reported studies in the BTE field are focused on bone formation, disregarding the entire bone remodeling steps, which also involve bone resorption. In this review, the currently available mono and co-culturing methods, types of biomaterials used in several strategies that combine scaffolds and relevant cells (e.g., osteoblasts (OBs), osteoclasts (OCs), and osteocytes (OCys)), envisioning a healthy bone formation and remodeling process, the gold-standard drug delivery systems, and bioengineered-based systems to tackle bone diseases are described.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"25 4","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and Degradation Study of PLA-Based Medical Implant Markers for Magnetic Particle Imaging 基于pla的医用植入物磁颗粒成像标记物的研制与降解研究。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2025-02-06 DOI: 10.1002/mabi.202400495

Patrick N. Elfers, Kerstin Lüdtke-Buzug, Ankit Malhotra, Justin Ackers, Liana Mirzojan, Maximilian Wattenberg, Johann C. Engster, David Melenberg, Mandy Ahlborg, Thomas Friedrich, Maria-Josephina Buhné, Malte M. Sieren, Thorsten M. Buzug, Roman Kloeckner, Jörg Barkhausen, Franz Wegner

{"title":"Development and Degradation Study of PLA-Based Medical Implant Markers for Magnetic Particle Imaging","authors":"Patrick N. Elfers, Kerstin Lüdtke-Buzug, Ankit Malhotra, Justin Ackers, Liana Mirzojan, Maximilian Wattenberg, Johann C. Engster, David Melenberg, Mandy Ahlborg, Thomas Friedrich, Maria-Josephina Buhné, Malte M. Sieren, Thorsten M. Buzug, Roman Kloeckner, Jörg Barkhausen, Franz Wegner","doi":"10.1002/mabi.202400495","DOIUrl":"10.1002/mabi.202400495","url":null,"abstract":"Magnetic particle imaging (MPI) is a promising imaging modality nearing clinical introduction. MPI's tracer-based principle allows for highly sensitive background-free imaging. Potential clinical applications include cardiovascular imaging and endovascular interventions. In principle, medical instruments are invisible in MPI due to the missing signal generation. Therefore, permanent marking technologies have been introduced. Additionally, temporary markers are of interest for follow-up examinations after stent implantation to prevent artifacts during postinterventional stent lumen quantification. Consequently, medical instrument markers for MPI, based on biodegradable polylactic acid (PLA) and superparamagnetic iron-oxide nanoparticles (SPIONs), are developed in this study. To investigate the markers, signal characteristics and degradation over time are studied for 28 d in a water bath at 37 °C. The samples are analyzed using a scale, micro-CT, microscopy, magnetic particle spectroscopy (MPS), MPI, and vibrating sample magnetometry (VSM). A continuous mass decrease is detected (≈90% after 28 d), while MPS and MPI data show no loss of signal. VSM confirms that the markers’ mass reduction can be accounted for the degradation of PLA, while the SPIONs hardly detach from the coating. The introduced marking technology, with its degradation characteristics and signal behavior, is the basis for a variety of anticipated medical application scenarios.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":"25 4","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202400495","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143255772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0