Biohybrid Living Material with Antibacterial and Regenerating Properties Based on Probiotic Bacteria Stress Metabolism Modulation.

Abstract

Wound healing is an intricate process that involves various biochemical pathways at each stage of tissue regeneration. Wound therapy is a series of distinct treatment stages that has a limited efficacy if wounds are of complex etiologies. A modern approach to this problem may be the development of bifunctional adaptive biohybrid systems that can concurrently affect pathogens' growth, inflammation, and tissue regeneration. We have developed biohybrid living material with antibacterial and regenerating properties based on induced hormesis by oxidative stress onto probiotic bacteria with prolonged synthesis of hydrogen peroxide, increased antibacterial action, and regeneration of the burn wound. Material demonstrates almost complete wound healing with a wound area difference 3-4 times with natural healing in vivo burn wound model for 21 days, antibacterial activity against wound antibiotic-resistance pathogens Escherichia coli K12 and Staphylococcus aureus ATCC 29213 in 4 and 5-fold, respectively in co-cultivation model, and has no toxicity to human skin fibroblasts and β-hemolysis in the in vitro model. Our findings promise the improving tissue regeneration of burn wounds, therapy against antibiotic-resistance pathogens by eliminating antibiotics, and other classical bactericides.