Macromolecular bioscience最新文献_第9页

Composite Hydrogel Containing Collagen-Modified Polylactic Acid-Hydroxylactic Acid Copolymer Microspheres Loaded with Tetramethylpyrazine Promotes Articular Cartilage Repair 含胶原改性聚乳酸-羟基乳酸共聚物微球并负载四甲基吡嗪的复合水凝胶可促进关节软骨修复

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-04-10 DOI: 10.1002/mabi.202400003

Yalan Pan, Bin Li, Xiaoxian Sun, Pengcheng Tu, Yang Guo, Zitong Zhao, Mao Wu, Yun Wang, Zhifang Wang, Yong Ma

{"title":"Composite Hydrogel Containing Collagen-Modified Polylactic Acid-Hydroxylactic Acid Copolymer Microspheres Loaded with Tetramethylpyrazine Promotes Articular Cartilage Repair","authors":"Yalan Pan, Bin Li, Xiaoxian Sun, Pengcheng Tu, Yang Guo, Zitong Zhao, Mao Wu, Yun Wang, Zhifang Wang, Yong Ma","doi":"10.1002/mabi.202400003","DOIUrl":"10.1002/mabi.202400003","url":null,"abstract":"Articular cartilage defects pose a significant challenge due to the limited self-healing ability of cartilage. However, traditional techniques face limitations including autologous chondrocyte expansion issues. This study aims to investigate the effects of the polylactic acid-glycolic acid (PLGA) and collagen-surface modified polylactic acid-glycolic acid (CPLGA) microspheres loaded with tetramethylpyrazine (TMP) on two cell types and the regeneration potential of articular cartilage. CPLGA microspheres are prepared by Steglich reaction and characterized. They evaluated the effect of TMP-loaded microspheres on HUVECs (Human Umbilical Vein Endothelial Cells) and examined the compatibility of blank microspheres with BMSCs (Bone marrow mesenchymal stromal cells) and their potential to promote cartilage differentiation. Subcutaneous implant immune tests and cartilage defect treatment are conducted to assess biocompatibility and cartilage repair potential. The results highlight the efficacy of CPLGA microspheres in promoting tissue regeneration, attributed to improved hydrophilicity and collagen-induced mitigation of degradation. Under hypoxic conditions, both CPLGA and PLGA TMP-loaded microspheres exhibit inhibitory effects on HUVEC proliferation, migration, and angiogenesis. Notably, CPLGA microspheres show enhanced compatibility with BMSCs, facilitating chondrogenic differentiation. Moreover, the CPLGA microsphere-composite hydrogel exhibits potential for cartilage repair by modulating angiogenesis and promoting BMSC differentiation.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140597136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Near-Infrared Triggered Biodegradable Microneedle Patch for Controlled Macromolecule Drug Release 用于控制大分子药物释放的近红外触发式生物可降解微针贴片

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-04-09 DOI: 10.1002/mabi.202400105

Yifan Cheng, Junzhu Yang, Sanyang Han, Yuan Lu

{"title":"Near-Infrared Triggered Biodegradable Microneedle Patch for Controlled Macromolecule Drug Release","authors":"Yifan Cheng, Junzhu Yang, Sanyang Han, Yuan Lu","doi":"10.1002/mabi.202400105","DOIUrl":"10.1002/mabi.202400105","url":null,"abstract":"Transdermal drug delivery of macromolecule drugs attracts significant attention due to the advantage of convenience and biocompatibility. However, the practical usage of it is limited by the low delivery efficiency and poor drug absorption. To develop an efficient, safe, and controllable transdermal delivery method, the near-infrared (NIR) triggered calcium sulfate and gelatin biodegradable composite microneedle (MN) patches are developed. The MN patches are fabricated by polydimethylsiloxane (PDMS) molds, and the structure data can be adjusted by changing the molds. Such an MN patch can release both macro and micro molecule drugs. After loading with photothermal converter IR780, which can transfer energy of light to heat, the release of macromolecule drugs in MNs can be controlled by applying NIR irradiation. The control effect can be enhanced by spraying 1-tetradecanol (TD) coating and optimizing the ratio (weight) of gelatin and calcium sulfate to 2:6. Besides, the MN patch can deliver drugs through the skin barrier, and the process can be controlled by NIR. Moreover, the insulin-loaded MN patch exhibits some therapeutic effects on healthy mice. This work suggests that biodegradable MNs can achieve controllable drug delivery and potentially be applied in individual treatment via transdermal ingestion.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140597024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monodisperse Polyaspartic Acid Derivative Microspheres for Potential Tumor Embolization Therapy 用于潜在肿瘤栓塞疗法的单分散聚天冬氨酸衍生物微球

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-04-08 DOI: 10.1002/mabi.202400047

Anqi Xu, Yuchen Sun, Mingyu Guo

引用次数: 0

Injectable Hydrogels: A Paradigm Tailored with Design, Characterization, and Multifaceted Approaches 可注射水凝胶：通过设计、表征和多方面方法量身定制的范例

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-04-05 DOI: 10.1002/mabi.202400049

Rishika Singhal, Manoj Kumar Sarangi, Goutam Rath

{"title":"Injectable Hydrogels: A Paradigm Tailored with Design, Characterization, and Multifaceted Approaches","authors":"Rishika Singhal, Manoj Kumar Sarangi, Goutam Rath","doi":"10.1002/mabi.202400049","DOIUrl":"10.1002/mabi.202400049","url":null,"abstract":"Biomaterials denoting self-healing and versatile structural integrity are highly curious in the biomedicine segment. The injectable and/or printable 3D printing technology is explored in a few decades back, which can alter their dimensions temporarily under shear stress, showing potential healing/recovery tendency with patient-specific intervention toward the development of personalized medicine. Thus, self-healing injectable hydrogels (IHs) are stunning toward developing a paradigm for tissue regeneration. This review comprises the designing of IHs, rheological characterization and stability, several benchmark consequences for self-healing IHs, their translation into tissue regeneration of specific types, applications of IHs in biomedical such as anticancer and immunomodulation, wound healing and tissue/bone regeneration, antimicrobial potentials, drugs, gene and vaccine delivery, ocular delivery, 3D printing, cosmeceuticals, and photothermal therapy as well as in other allied avenues like agriculture, aerospace, electronic/electrical industries, coating approaches, patents associated with therapeutic/nontherapeutic avenues, and numerous futuristic challenges and solutions.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140597031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polydopamine-Mediated Metal–Organic Frameworks Modification for Improved Biocompatibility 聚多巴胺介导的金属有机框架改性可提高生物相容性

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-04-03 DOI: 10.1002/mabi.202400071

Jiayu Feng, Liwang Xu, Lulu Qi, Zhengwei Fu, Qinglian Hu

{"title":"Polydopamine-Mediated Metal–Organic Frameworks Modification for Improved Biocompatibility","authors":"Jiayu Feng, Liwang Xu, Lulu Qi, Zhengwei Fu, Qinglian Hu","doi":"10.1002/mabi.202400071","DOIUrl":"10.1002/mabi.202400071","url":null,"abstract":"Engineered nanomaterials are promising in biomedical application. However, insufficient understanding of their biocompatibility at the cellular and organic levels prevents their widely biomedical applications. Metal–organic frameworks (MOFs) have attracted increasing attention in recent years. In this work, zeolitic imidazolate framework-8 (ZIF-8) and polydopamine (PDA)-modified ZIF-8 are chosen as model nanomaterials due to its emergent role in nanomedicine. In vitro, the results demonstrate that the PDA coating greatly alleviates the cytotoxicity of ZIF-8 to RAW264.7, LO2, and HST6, which represent three different cell types in liver organs. Mechanistically, ZIF-8 entering into the cells can greatly induce the reactive oxygen species generation, which subsequently induces cell cycle delay and autophagy, ultimately leads to enhanced cytotoxicity. Further, human umbilical vein endothelial cells model and zebrafish embryos assay also confirm that PDA can compromise the ZIF-8 toxicity significantly. This study reveals that PDA-coated MOFs nanomaterials show great potential in nano-based drug delivery systems\u0000.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140597030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peptoid-Cross-Linked Hydrogel Stiffness Modulates Human Mesenchymal Stromal Cell Immunoregulatory Potential in the Presence of Interferon-Gamma 蛋白胨交联水凝胶的硬度可在干扰素-γ的作用下调节人间质基质细胞的免疫调节潜能

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-04-03 DOI: 10.1002/mabi.202400111

David A. Castilla-Casadiego, Logan D. Morton, Darren H. Loh, Aldaly Pineda-Hernandez, Ajay P. Chavda, Francis Garcia, Adrianne M. Rosales

{"title":"Peptoid-Cross-Linked Hydrogel Stiffness Modulates Human Mesenchymal Stromal Cell Immunoregulatory Potential in the Presence of Interferon-Gamma","authors":"David A. Castilla-Casadiego, Logan D. Morton, Darren H. Loh, Aldaly Pineda-Hernandez, Ajay P. Chavda, Francis Garcia, Adrianne M. Rosales","doi":"10.1002/mabi.202400111","DOIUrl":"10.1002/mabi.202400111","url":null,"abstract":"Human mesenchymal stromal cell (hMSC) manufacturing requires the production of large numbers of therapeutically potent cells. Licensing with soluble cytokines improves hMSC therapeutic potency by enhancing secretion of immunoactive factors but typically decreases proliferative ability. Soft hydrogels, however, have shown promise for boosting immunomodulatory potential, which may compensate for decreased proliferation. Here, hydrogels are cross-linked with peptoids of different secondary structures to generate substrates of various bulk stiffnesses but fixed network connectivity. Secretions of interleukin 6, monocyte chemoattractive protein-1, macrophage colony-stimulating factor, and vascular endothelial growth factor are shown to depend on hydrogel stiffness in the presence of interferon gamma (IFN-γ) supplementation, with soft substrates further improving secretion. The immunological function of these secreted cytokines is then investigated via coculture of hMSCs seeded on hydrogels with primary peripheral blood mononuclear cells (PBMCs) in the presence and absence of IFN-γ. Cocultures with hMSCs seeded on softer hydrogels show decreased PBMC proliferation with IFN-γ. To probe possible signaling pathways, immunofluorescent studies probe the nuclear factor kappa B pathway and demonstrate that IFN-γ supplementation and softer hydrogel mechanics lead to higher activation of this pathway. Overall, these studies may allow for production of more efficacious therapeutic hMSCs in the presence of IFN-γ.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140597028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peptide Sequence Variations Govern Hydrogel Stiffness: Insights from a Multi-Scale Structural Analysis of H-FQFQFK-NH2 Peptide Derivatives 多肽序列变化控制水凝胶硬度：对 H-FQFQFK-NH2 肽衍生物进行多尺度结构分析的启示。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-03-29 DOI: 10.1002/mabi.202300579

Tess De Maeseneer, Thibault Cauwenbergh, James Gardiner, Jacinta F. White, Wim Thielemans, Charlotte Martin, Paula Moldenaers, Steven Ballet, Ruth Cardinaels

{"title":"Peptide Sequence Variations Govern Hydrogel Stiffness: Insights from a Multi-Scale Structural Analysis of H-FQFQFK-NH2 Peptide Derivatives","authors":"Tess De Maeseneer, Thibault Cauwenbergh, James Gardiner, Jacinta F. White, Wim Thielemans, Charlotte Martin, Paula Moldenaers, Steven Ballet, Ruth Cardinaels","doi":"10.1002/mabi.202300579","DOIUrl":"10.1002/mabi.202300579","url":null,"abstract":"Throughout the past decades, amphipathic peptide-based hydrogels have proven to be promising materials for biomedical applications. Amphipathic peptides are known to adopt β-sheet configurations that self-assemble into fibers that then interact to form a hydrogel network. A fundamental understanding of how the peptide sequence alters the structural properties of the hydrogels would allow for a more rational design of novel peptides for a variety of biomedical applications in the future. Therefore, the current work investigates how changing the type of amino acid, the amphipathic pattern, and the peptide length affects the secondary structure, fiber characteristics, and stiffness of peptide-based hydrogels. Hereto, seven amphipathic peptides of different sequence and length, four of which have not been previously reported, based on and including the hexapeptide H-Phe-Gln-Phe-Gln-Phe-Lys-NH2, are synthesized and thoroughly characterized by circular dichroism (CD), Fourier Transform Infrared (FTIR) spectroscopy, Wide Angle X-ray Scattering (WAXS), Small Angle X-ray Scattering (SAXS), Transmission Electron Microscopy (TEM), and Thioflavin T (ThT) fibrillization assays. The results show that a high amount of regularly spaced β-sheets, a high amount of fibers, and fiber bundling contribute to the stiffness of the hydrogel. Furthermore, a study of the time-dependent fibril formation process reveals complex transient dynamics. The peptide strands structure through an intermediate helical state prior to β-sheet formation, which is found to be concentration- and time-dependent.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202300579","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140326784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing the Potential of Self-Assembled Peptide Hydrogels for Neural Regeneration and Tissue Engineering 利用自组装多肽水凝胶的潜力促进神经再生和组织工程。

IF 4.6 4区医学

Macromolecular bioscience Pub Date : 2024-03-28 DOI: 10.1002/mabi.202300534

Haniyeh Najafi, Ghazal Farahavar, Mahboobeh Jafari, Samira Sadat Abolmaali, Negar Azarpira, Ali Mohammad Tamaddon

引用次数: 0

Additive Manufacturing of Wet-Spun Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-Based Scaffolds Loaded with Hydroxyapatite 加载羟基磷灰石的湿法纺丝聚（3-羟基丁酸-co-3-羟基戊酸）基支架的加成制造

IF 4.6 4区医学

Macromolecular bioscience Pub Date : 2024-03-27 DOI: 10.1002/mabi.202300538

Gianni Pecorini, Simona Braccini, Stefano Simoni, Andrea Corti, Gianluca Parrini, Dario Puppi

{"title":"Additive Manufacturing of Wet-Spun Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-Based Scaffolds Loaded with Hydroxyapatite","authors":"Gianni Pecorini, Simona Braccini, Stefano Simoni, Andrea Corti, Gianluca Parrini, Dario Puppi","doi":"10.1002/mabi.202300538","DOIUrl":"10.1002/mabi.202300538","url":null,"abstract":"Tissue engineering represents an advanced therapeutic approach for the treatment of bone tissue defects. Polyhydroxyalkanoates are a promising class of natural polymers in this context thanks to their biocompatibility, processing versatility, and mechanical properties. The aim of this study is the development by computer-aided wet-spinning of novel poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)-based composite scaffolds for bone engineering. In particular, PHBV scaffolds are loaded with hydroxyapatite (HA), an osteoinductive ceramic, in order to tailor their biological activity and mechanical properties. PHBV blending with poly(lactide-co-glycolide) (PLGA) is also explored to increase the processing properties of the polymeric mixture used for composite scaffold fabrication. Different HA percentages, up to 15% wt., can be loaded into the PHBV or PHBV/PLGA scaffolds without compromising their interconnected porous architecture, as well as the polymer morphological and thermal properties, as demonstrated by scanning electron microscopy, thermogravimetric analysis, and differential scanning calorimetry. In addition, HA loading results in increased scaffold compressive stiffness to levels comparable to those of trabecular bone tissue, as well as in higher in vitro MC3T3-E1 cell viability and production of mineralized extracellular matrix, in comparison to what observed for unloaded scaffolds. The observed mechanical and biological properties suggest the suitability of the developed scaffolds for bone engineering.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140293923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design and Fabrication of Broad-Spectrum Antimicrobial Porous Metallo-Polymeric Microsphere for Water Disinfection 设计和制造用于水消毒的广谱抗菌多孔金属聚合物微球

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-03-23 DOI: 10.1002/mabi.202400004

Anil R. Gupta, Monika Rank, Aneesha Singh, Saroj Sharma

{"title":"Design and Fabrication of Broad-Spectrum Antimicrobial Porous Metallo-Polymeric Microsphere for Water Disinfection","authors":"Anil R. Gupta, Monika Rank, Aneesha Singh, Saroj Sharma","doi":"10.1002/mabi.202400004","DOIUrl":"10.1002/mabi.202400004","url":null,"abstract":"An expedient and efficient approach is used to synthesize a new class of metallo-polymeric microspheres (MPMs) as antimicrobials to succumb the wide range of bacteria from water. Three types of MPMs, that is, poly[Silver (I)-methacrylate-co-methylmethacrylate] (pAgMA), poly[Copper (II)-methacrylate-co-methyl methacrylate] (pCuMA), and poly[Nickel (II)-methacrylate-co-methylmethacrylate] (pNiMA), are prepared via radical suspension polymerization technique in 3D shape with porous texture. The structural and morphological characterization of the prepared microspheres are examined by analytical techniques. The antimicrobial potentialities of prepared MPMs are investigated at the laboratory scale study, revealing that the MPMs exhibit strong antibacterial activity (≈99.9% killing) against Gram-negative and Gram-positive bacteria [Enterobacter hormaechei (EH), Bacillus megatarium (BM), and Bacillus bataviensis (BB)]. The MacConkey agar medium test reveals that MPMs have substantial biocidal efficacy against broad-spectrum Gram-negative bacteria present in tap water. The MPMs exhibit significant antimicrobial efficacy via contact killing owe to the presence of integrated biocidal metal moiety, which represents that the MPMs are safe for water disinfection.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140194054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0