Macromolecular bioscience最新文献_第9页

Unlocking Novel Anticancer Strategies: Bioactive Hydrogels for Local Delivery of Plasma-Derived Oxidants in an In Ovo Cancer Model. 开启新的抗癌策略：生物活性水凝胶用于在卵内癌症模型中局部输送血浆氧化剂

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-06-20 DOI: 10.1002/mabi.202400213

Albert Espona-Noguera, Milica Živanić, Evelien Smits, Annemie Bogaerts, Angela Privat-Maldonado, Cristina Canal

{"title":"Unlocking Novel Anticancer Strategies: Bioactive Hydrogels for Local Delivery of Plasma-Derived Oxidants in an In Ovo Cancer Model.","authors":"Albert Espona-Noguera, Milica Živanić, Evelien Smits, Annemie Bogaerts, Angela Privat-Maldonado, Cristina Canal","doi":"10.1002/mabi.202400213","DOIUrl":"10.1002/mabi.202400213","url":null,"abstract":"Cold atmospheric plasma (CAP) is a tool with the ability to generate reactive oxygen and nitrogen species (RONS), which can induce therapeutic effects like disinfection, wound healing, and cancer treatment. In the plasma oncology field, CAP-treated hydrogels (PTHs) are being explored for the local administration of CAP-derived RONS as a novel anticancer approach. PTHs have shown anticancer effects in vitro, however, they have not yet been studied in more relevant cancer models. In this context, the present study explores for the first time the therapeutic potential of PTHs using an advanced in ovo cancer model. PTHs composed of alginate (Alg), gelatin (Gel), Alg/Gel combination, or Alg/hyaluronic acid (HA) combination are investigated. All embryos survived the PTHs treatment, suggesting that the in ovo model could become a time- and cost-effective tool for developing hydrogel-based anticancer approaches. Results revealed a notable reduction in CD44+ cell population and their proliferative state for the CAP-treated Alg-HA condition. Moreover, the CAP-treated Alg-HA formulation alters the extracellular matrix composition, which may help combat drug-resistance. In conclusion, the present study validates the utility of in ovo cancer model for PTHs exploration and highlights the promising potential of Alg-based PTHs containing HA and CAP-derived RONS for cancer treatment.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141427167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

pH-Responsive Degradable Electro-Spun Nanofibers Crosslinked via Boronic Ester Chemistry for Smart Wound Dressings 通过硼酸酯化学交联的 pH 值响应型可降解电纺纳米纤维用于智能伤口敷料

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-06-20 DOI: 10.1002/mabi.202400217

Sofia Nieves Casillas-Popova, Nishadi Dilkushi Lokuge, Brandon Andrade-Gagnon, Farhan Rahman Chowdhury, Cameron D. Skinner, Brandon L. Findlay, Jung Kwon Oh

{"title":"pH-Responsive Degradable Electro-Spun Nanofibers Crosslinked via Boronic Ester Chemistry for Smart Wound Dressings","authors":"Sofia Nieves Casillas-Popova, Nishadi Dilkushi Lokuge, Brandon Andrade-Gagnon, Farhan Rahman Chowdhury, Cameron D. Skinner, Brandon L. Findlay, Jung Kwon Oh","doi":"10.1002/mabi.202400217","DOIUrl":"10.1002/mabi.202400217","url":null,"abstract":"Recent advances in the treatment of chronic wounds have focused on the development of effective strategies for cutting-edge wound dressings based on nanostructured materials, particularly biocompatible poly(vinyl alcohol) (PVA)-based electro-spun (e-spun) nanofibers. However, PVA nanofibers need to be chemically crosslinked to ensure their dimensional stability in aqueous environment and their capability to encapsulate bioactive molecules. Herein, a robust approach for the fabrication of pH-degradable e-spun PVA nanofibers crosslinked with dynamic boronic ester (BE) linkages through a coupling reaction of PVA hydroxyl groups with the boronic acid groups of a phenyl diboronic acid crosslinker is reported. This comprehensive analysis reveals the importance of the mole ratio of boronic acid to hydroxyl group for the fabrication of well-defined BE-crosslinked fibrous mats with not only dimensional stability but also the ability to retain uniform fibrous form in aqueous solutions. These nanofibers degrade in both acidic and basic conditions that mimic wound environments, leading to controlled/enhanced release of encapsulated antimicrobial drug molecules. More importantly, drug-loaded BE-crosslinked fibers show excellent antimicrobial activities against both Gram-positive and Gram-negative bacteria, suggesting that this approach of exploring dynamic BE chemistry is amenable to the development of smart wound dressings with controlled/enhanced drug release.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202400217","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141523050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Avastin-Loaded 3D-Printed Alginate Scaffold as an Effective Antiadhesive Barrier to Prevent Postsurgical Adhesion Bands Formation 阿瓦斯汀负载的三维打印藻酸盐支架是防止术后粘连带形成的有效抗粘连屏障

IF 4.6 4区医学

Macromolecular bioscience Pub Date : 2024-06-14 DOI: 10.1002/mabi.202470014

Saeid Vakilian, Sulaiman Al-Hashmi, Juhaina Al-kindi, Fahad Al-Fahdi, Nasar Al-Wahaibi, Asem Shalaby, Hamad Al-Riyami, Ahmed Al-Harrasi, Fatemeh Jamshidi-adegani

引用次数: 0

Masthead: Macromol. Biosci. 6/2024 刊头：Macromol.Biosci.6/2024

IF 4.6 4区医学

Macromolecular bioscience Pub Date : 2024-06-14 DOI: 10.1002/mabi.202470015

引用次数: 0

3D Puzzle at the Nanoscale–How do RNA Viruses Self-Assemble their Capsids into Perfectly Ordered Structures 纳米尺度上的三维难题--RNA 病毒如何将其外壳自我组装成完美有序的结构？

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-06-12 DOI: 10.1002/mabi.202400088

Martyna Kordys, Anna Urbanowicz

{"title":"3D Puzzle at the Nanoscale–How do RNA Viruses Self-Assemble their Capsids into Perfectly Ordered Structures","authors":"Martyna Kordys, Anna Urbanowicz","doi":"10.1002/mabi.202400088","DOIUrl":"10.1002/mabi.202400088","url":null,"abstract":"The phenomenon of RNA virus self-organization, first observed in the mid-20th century in tobacco mosaic virus, is the subject of extensive research. Efforts to comprehend this process intensify due to its potential for producing vaccines or antiviral compounds as well as nanocarriers and nanotemplates. However, direct observation of the self-assembly is hindered by its prevalence within infected host cells. One of the approaches involves in vitro and in silico research using model viruses featuring a ssRNA(+) genome enclosed within a capsid made up of a single type protein. While various pathways are proposed based on these studies, their relevance in vivo remains uncertain. On the other hand, the development of advanced microscopic methods provide insights into the events within living cells, where following viral infection, specialized compartments form to facilitate the creation of nascent virions. Intriguingly, a growing body of evidence indicates that the primary function of packaging signals in viral RNA is to effectively initiate the virion self-assembly. This is in contrast to earlier opinions suggesting a role in marking RNA for encapsidation. Another noteworthy observation is that many viruses undergo self-assembly within membraneless liquid organelles, which are specifically induced by viral proteins.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141306260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond the Charge: Interplay of Nanogels’ Functional Group and Zeta-Potential for Antifungal Drug Delivery to Human Pathogenic Fungus Aspergillus Fumigatus 超越电荷：纳米凝胶的功能基团与 Zeta 电位在向人类致病真菌曲霉菌输送抗真菌药物中的相互作用。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-06-08 DOI: 10.1002/mabi.202400082

Theresa Vogel, Simon Kohlmann, Zahraa Abboud, Sina Thusek, Franziska Fella, Joerg Teßmar, Kazuhisa Sekimizu, Atsushi Miyashita, Andreas Beilhack, Jürgen Groll, Yidong Yu, Krystyna Albrecht

{"title":"Beyond the Charge: Interplay of Nanogels’ Functional Group and Zeta-Potential for Antifungal Drug Delivery to Human Pathogenic Fungus Aspergillus Fumigatus","authors":"Theresa Vogel, Simon Kohlmann, Zahraa Abboud, Sina Thusek, Franziska Fella, Joerg Teßmar, Kazuhisa Sekimizu, Atsushi Miyashita, Andreas Beilhack, Jürgen Groll, Yidong Yu, Krystyna Albrecht","doi":"10.1002/mabi.202400082","DOIUrl":"10.1002/mabi.202400082","url":null,"abstract":"The ubiquitous mold Aspergillus fumigatus (A. fumigatus) is one of the main fungal pathogens causing invasive infections in immunocompromised humans. Conventional antifungal agents exhibit limited efficacy and often cause severe side effects. Nanoparticle-based antifungal delivery provides a promising alternative, which can increase local drug concentration; while, mitigating toxicity, thereby enhancing treatment efficacy. Previous research underscores the potential of poly(glycidol)-based nanogels (NG) with negative surface charge as carriers for delivering antifungals to A. fumigatus hyphae. In this study, NG is tailored with 2-carboxyethyl acrylate (CEA) or with phosphoric acid 2-hydroxyethyl acrylate (PHA). It is discovered that quenching with PHA clearly improves the adhesion of NG to hyphal surface and the internalization of NG into the hyphae under protein-rich conditions, surpassing the outcomes of non-quenched and CEA-quenched NG. This enhancement cannot be solely attributed to an increase in negative surface charge but appears to be contingent on the functional group of the quencher. Further, it is demonstrated that itraconazole-loaded, PHA-functionalized nanogels (NGxPHA-ITZ) show lower MIC in vitro and superior therapeutic effect in vivo against A. fumigatus compared to pure itraconazole. This confirms NGxPHA as a promising antifungal delivery system.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202400082","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141288255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Compressive Modulus of Porous PVA Hydrogel Coating on the Preventing Adhesion of Polypropylene Mesh 多孔 PVA 水凝胶涂层的压缩模量对防止聚丙烯网格粘附的影响

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-06-08 DOI: 10.1002/mabi.202400112

Dandan Wei, Yulin Huang, Pengfei Ren, Min Liang, Li Xu, Liuxin Yang, Tianzhu Zhang, Zhenling Ji

{"title":"Effect of Compressive Modulus of Porous PVA Hydrogel Coating on the Preventing Adhesion of Polypropylene Mesh","authors":"Dandan Wei, Yulin Huang, Pengfei Ren, Min Liang, Li Xu, Liuxin Yang, Tianzhu Zhang, Zhenling Ji","doi":"10.1002/mabi.202400112","DOIUrl":"10.1002/mabi.202400112","url":null,"abstract":"PP mesh is a widely used prosthetic material in hernia repair. However, visceral adhesion is one of the worst complications of this operation. Hence, an anti-adhesive PP mesh is developed by coating porous polyvinyl alcohol (PVA) hydrogel on PP surface via freezing-thawing process method. The compressive modulus of porous PVA hydrogel coating is first regulated by the addition of porogen sodium bicarbonate (NaHCO3) at various quality ratios with PVA. As expected, the porous hydrogel coating displayed modulus more closely resembling that of native abdominal wall tissue. In vitro tests demonstrate the modified PP mesh show superior coating stability, excellent hemocompatibility, and good cytocompatibility. In vivo experiments illustrate that PP mesh coated by the PVA4 hydrogel that mimicked the modulus of native abdominal wall could prevent adhesion effectively. Based on this, the rapamycin (RPM) is loaded into the porous PVA4 hydrogel coating to further improve anti-adhesive property of PP mesh. The Hematoxylin and eosin (H&E) and Masson trichrome (MT) staining results verified that the resulting mesh could alleviate the inflammation response and reduce the deposition of collagen around the implantation zone. The biomimetic mechanical property and anti-adhesive property of modified PP mesh make it a valuable candidate for application in hernioplasty.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141293530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulating Bacterial Culture through Tailored Silk Inverse Opal Scaffolds 通过定制的丝绸反向蛋白石支架调节细菌培养。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-06-06 DOI: 10.1002/mabi.202400238

Bei Qi, Yitan Li, Junyan Zhao, Jiapeng Zhang, Xiaohua Zhang, Gaojian Chen, Zhaohui Yang

引用次数: 0

Preparation of Autoclavable and Injectable Silk Fibroin Cryogels for Tissue Engineering Applications 制备用于组织工程应用的可高压灭菌和可注射的蚕丝纤维冷凝胶。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-06-06 DOI: 10.1002/mabi.202400038

Hongjuan Han, Haiyan Li, Lu Wang, Yong Zhu, Haoqing Guan, Jingzhi Yao, Wenqian Xiao, Bo Li, Xiaoling Liao

{"title":"Preparation of Autoclavable and Injectable Silk Fibroin Cryogels for Tissue Engineering Applications","authors":"Hongjuan Han, Haiyan Li, Lu Wang, Yong Zhu, Haoqing Guan, Jingzhi Yao, Wenqian Xiao, Bo Li, Xiaoling Liao","doi":"10.1002/mabi.202400038","DOIUrl":"10.1002/mabi.202400038","url":null,"abstract":"A cryogel is a supermacroporous gel network that is generated at subzero temperatures by polymerizing monomers or gelating polymeric precursors. Since cryogels possess inherent characteristics such as interconnected macroporous structures, excellent mechanical properties, and high resistance to autoclave sterilization, they are highly desirable for tissue engineering and regenerative medicine. Silk fibroin, a natural protein obtained from Bombyx mori silkworms, is an excellent raw material for cryogel preparation. The aim of this study is to establish a controlled method for preparing silk fibroin cryogels with suitable properties for application as tissue engineering scaffolds. Using a dual crosslinking strategy consisting of low-temperature radical polymerization coupled with methanol-induced conformational transformation, porous cryogels are prepared. The cryogels display many unique characteristics, such as an interconnected macroporous structure, a high water absorption capacity, water-triggered shape memory, syringe injectability, and strong resilience to autoclave sterilization. Furthermore, the cryogels demonstrate excellent biocompatibility and cell affinity, facilitating cell adhesion, migration, and proliferation. The interconnected supermacroporous architecture resembling the native extracellular matrix, together with their unique physical properties and autoclaving stability, suggests that cryogels are promising candidate scaffolds for tissue engineering and cell therapy.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141284129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Histidine Oligomers in Lipid Nanoparticles on siRNA Delivery 脂质纳米颗粒中的组氨酸低聚物对 siRNA 递送的影响

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-05-31 DOI: 10.1002/mabi.202400043

Hyeondo Lee, Gayeon You, Sangho Yeo, Hyukjin Lee, Hyejung Mok

{"title":"Effects of Histidine Oligomers in Lipid Nanoparticles on siRNA Delivery","authors":"Hyeondo Lee, Gayeon You, Sangho Yeo, Hyukjin Lee, Hyejung Mok","doi":"10.1002/mabi.202400043","DOIUrl":"10.1002/mabi.202400043","url":null,"abstract":"In this study, histidine oligomer (oHis; 10mer)-incorporating LNPs (H10LNPs) are developed as a novel carrier for efficient siRNA delivery. Notably, the unmodified oHis (10mer) is greatly incorporated within LNPs through ionic interaction with siRNAs, which serves as an endosome escape enhancer. H10LNPs with a size of ≈65 nm demonstrate a significantly enhanced extent of endosomal escape, as evidenced by calcein assay and confocal microscopy images of intracellular fluorescence, surpassing conventional LNPs. Furthermore, the half inhibitory concentration (IC50) of the human endogenous globotriaosylceramide synthase (Gb3 synthase) gene in H10LNPs-treated cells exhibits a significant threefold decrease, compared to that in LNP-treated cells. Notably, H10LNPs maintain comparable biocompatibility and biodistribution both in vitro and in vivo. Considering that the fabricated siRNA H10LNPs exhibit excellent biocompatibility and superior gene silencing activity over conventional LNPs, these particles can be harnessed for the safe delivery of therapeutic siRNAs. Additionally, this study introduces promising, feasible, simple, and alternative formulation processes for integrating unmodified functional cationic peptides into LNPs to enhance the delivery efficiency of a wide range of nucleic acid-based drugs.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141180441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0