Magnetic Levitational Assembly of Differentiated SH-SY5Y Cells for Aβ-Induced 3D Alzheimer's Disease Modeling and Curcumin Screening.

IF 4.4 4区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Macromolecular bioscience Pub Date : 2025-03-25 DOI:10.1002/mabi.202400658

Rumeysa Bilginer-Kartal, Ahu Arslan-Yildiz

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引用次数: 0

Abstract

Alzheimer's disease is one of the prevalent neurodegenerative diseases and is characterized by amyloid beta aggregate (Aβ) accumulation. This study reports an Aβ 1-42 induced 3D Alzheimer's disease modeling utilizing differentiated SH-SY5Y spheroids, which is carried out by Magnetic levitation approach, and the neuroprotective effect of Curcumin is further investigated on this model. For this purpose, SH-SY5Y spheroids are differentiated using Retinoic acid-Brain-derived neurotrophic factor sequentially during 3D cell culture. Differentiated spheroids maintained high viability and exhibited significant neuronal characteristics, as evidenced by increasing β-III tubulin and NeuN expressions. 3D Alzheimer's disease model formation and neurotoxicity of Aβ 1-42 aggregates are investigated on un-/differentiated spheroids, resulting in 65% and 51% cell viability, respectively. Characterization of the 3D Alzheimer's disease model is done by immunostaining of Choline acetyltransferase to investigate cholinergic neuron activity loss, showing a 2.2 decrease in fluorescence intensity. Further, Curcumin treatment on the 3D Alzheimer's disease model resulted in augmenting cell viability, confirming neuroprotective effect of Curcumin on Aβ 1-42 induced Alzheimer's disease model. This study highlighted the magnetic levitation-based fabrication of Aβ 1-42-induced 3D Alzheimer's disease model successfully, offering a promising experimental platform for other neurodegenerative disease research and potential clinical applications.

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a β诱导阿尔茨海默病三维建模及姜黄素筛选的SH-SY5Y分化细胞磁悬浮组装

阿尔茨海默病是一种常见的神经退行性疾病，其特征是β淀粉样蛋白聚集体（Aβ）积累。本研究采用磁悬浮方法，利用SH-SY5Y分化球体建立了Aβ 1-42诱导的阿尔茨海默病三维模型，并进一步研究了姜黄素对该模型的神经保护作用。为此，在3D细胞培养过程中，使用视黄酸-脑源性神经营养因子依次分化SH-SY5Y球体。分化后的球体保持了较高的活力，并表现出明显的神经元特征，β-III微管蛋白和NeuN的表达增加。在未分化球体上研究了3D阿尔茨海默病模型的形成和Aβ 1-42聚集体的神经毒性，分别导致65%和51%的细胞存活率。三维阿尔茨海默病模型的表征是通过胆碱乙酰转移酶免疫染色来研究胆碱能神经元活动的丧失，显示荧光强度下降2.2。此外，姜黄素对3D阿尔茨海默病模型的治疗导致细胞活力增强，证实了姜黄素对Aβ 1-42诱导的阿尔茨海默病模型的神经保护作用。本研究成功实现了基于磁悬浮技术制备β 1-42诱导的阿尔茨海默病3D模型，为其他神经退行性疾病的研究和潜在的临床应用提供了良好的实验平台。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Macromolecular bioscience 生物-材料科学：生物材料

CiteScore

7.90

自引率

2.20%

发文量

211

审稿时长

1.5 months

期刊介绍： Macromolecular Bioscience is a leading journal at the intersection of polymer and materials sciences with life science and medicine. With an Impact Factor of 2.895 (2018 Journal Impact Factor, Journal Citation Reports (Clarivate Analytics, 2019)), it is currently ranked among the top biomaterials and polymer journals. Macromolecular Bioscience offers an attractive mixture of high-quality Reviews, Feature Articles, Communications, and Full Papers. With average reviewing times below 30 days, publication times of 2.5 months and listing in all major indices, including Medline, Macromolecular Bioscience is the journal of choice for your best contributions at the intersection of polymer and life sciences.