Macromolecular bioscience最新文献_第8页

Masthead: Macromol. Biosci. 7/2024 刊头：Macromol.Biosci.7/2024

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-07-15 DOI: 10.1002/mabi.202470017

引用次数: 0

Optimization of Zwitterionic Polymers for Cell Cryopreservation 优化用于细胞冷冻保存的聚合离子聚合物

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-07-15 DOI: 10.1002/mabi.202470016

Yui Kato, Takuya Uto, Takeru Ishizaki, Daisuke Tanaka, Kojiro Ishibashi, Yuya Matsuda, Issei Onoda, Akiko Kobayashi, Masaharu Hazawa, Richard W. Wong, Kenji Takahashi, Eishu Hirata, Kosuke Kuroda

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Challenges and Opportunities in Developing Tracheal Substitutes for the Recovery of Long-Segment Defects 开发用于恢复长段缺损的气管替代物的挑战与机遇。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-07-15 DOI: 10.1002/mabi.202400054

Kausik Kapat, Prashil Gondane, Sakshi Kumbhakarn, Shruti Takle, Rahul Sable

{"title":"Challenges and Opportunities in Developing Tracheal Substitutes for the Recovery of Long-Segment Defects","authors":"Kausik Kapat, Prashil Gondane, Sakshi Kumbhakarn, Shruti Takle, Rahul Sable","doi":"10.1002/mabi.202400054","DOIUrl":"10.1002/mabi.202400054","url":null,"abstract":"Tracheal resection and reconstruction procedures are necessary when stenosis, tracheomalacia, tumors, vascular lesions, or tracheal injury cause a tracheal blockage. Replacement with a tracheal substitute is often recommended when the trauma exceeds 50% of the total length of the trachea in adults and 30% in children. Recently, tissue engineering and other advanced techniques have shown promise in fabricating biocompatible tracheal substitutes with physical, morphological, biomechanical, and biological characteristics similar to native trachea. Different polymers and biometals are explored. Even with limited success with tissue-engineered grafts in clinical settings, complete healing of tracheal defects remains a substantial challenge due to low mechanical strength and durability of the graft materials, inadequate re-epithelialization and vascularization, and restenosis. This review has covered a range of reconstructive and regenerative techniques, design criteria, the use of bioprostheses and synthetic grafts for the recovery of tracheal defects, as well as the traditional and cutting-edge methods of their fabrication, surface modification for increased immuno- or biocompatibility, and associated challenges.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141620307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Thermosensitive Hydrogels as Targeted and Controlled Drug Delivery Systems: Potential Applications in Transplantation 热敏水凝胶作为靶向和可控的给药系统：移植中的潜在应用。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-07-11 DOI: 10.1002/mabi.202400064

Madonna Rica Anggelia, Hui-Yun Cheng, Cheng-Hung Lin

引用次数: 0

Design and Preparation of Lifetime-Based Dual Fluorescent/Phosphorescent Sensor of pH and Oxygen and its Exploration in Model Physiological Solutions and Cells 基于生命周期的 pH 和氧气双荧光/磷光传感器的设计和制备及其在模型生理溶液和细胞中的应用探索。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-07-10 DOI: 10.1002/mabi.202400225

Vadim Baigildin, Julia Shakirova, Nina Zharskaia, Elena Ivanova, Sergey Silonov, Viktor Sokolov, Sergey Tunik

{"title":"Design and Preparation of Lifetime-Based Dual Fluorescent/Phosphorescent Sensor of pH and Oxygen and its Exploration in Model Physiological Solutions and Cells","authors":"Vadim Baigildin, Julia Shakirova, Nina Zharskaia, Elena Ivanova, Sergey Silonov, Viktor Sokolov, Sergey Tunik","doi":"10.1002/mabi.202400225","DOIUrl":"10.1002/mabi.202400225","url":null,"abstract":"In the present report, a novel dual pH-O2 sensor based on covalent conjugate of rhodamine 6G and cyclometalated iridium complex with poly(vinylpyrrolidone-block-vinyltetrazole) copolymer is reported. In model physiological solutions the sensor chromophores display independent phosphorescent and fluorescent lifetime responses onto variations in oxygen concentration and pH, respectively. Colocalization studies on Chinese hamster ovary cells demonstrate the preferential localization in endosomes and lysosomes. The fluorescent lifetime imaging microscopy-phosphorescent lifetime imaging microscopy (FLIM-PLIM) experiments show that the phosphorescent O2 sensor provides unambiguous information onto hypoxia versus normoxia cell status as well as semi-quantitative data on the oxygen concentration in cells in between these two states. However, the results of FLIM measurements indicate that dynamic lifetime interval of the sensor (≈0.5 ns between pH values 5.0 and 8.0) is insufficient even for qualitative estimation of pH in living cells because half-width of lifetime distribution in the studied samples is higher than the sensor dynamic interval. Nevertheless, the variations in rhodamine emission intensity are much higher and allow rough discrimination of acidic and neutral cell conditions. Thus, the results of this study indicate that the suggested approach to the design of dual pH-O2 sensors makes possible to prepare the biocompatible and water-soluble conjugate with fast cellular uptake.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141580125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Co-Delivery of Oxygen and Anticancer Drugs on the Viability of Healthy and Cancer Cells under Normoxic and Hypoxic Conditions 常氧和缺氧条件下氧气和抗癌药物共同输送对健康细胞和癌细胞活力的影响

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-07-09 DOI: 10.1002/mabi.202400181

Nermin Seda Kehr

{"title":"The Effect of Co-Delivery of Oxygen and Anticancer Drugs on the Viability of Healthy and Cancer Cells under Normoxic and Hypoxic Conditions","authors":"Nermin Seda Kehr","doi":"10.1002/mabi.202400181","DOIUrl":"10.1002/mabi.202400181","url":null,"abstract":"Hypoxia, cancer, tissue damage, and acidic pH conditions are interrelated, as chronic hypoxic conditions enhance the malignant phenotype of cancer cells, causing more aggressive tissue destruction, and hypoxic cells rely on anaerobic glycolysis, leading to the accumulation of lactic acid. Therefore, the administration of oxygen is necessary to support the functions of healthy cells until the formation of new blood vessels and to increase the oxygen supply to cancerous tissues to improve the efficacy of antitumor drugs on tumor cells. In addition to O2 supply, pH-dependent delivery of anticancer drugs is desired to target cancer cells and reduce drug side effects on healthy cells. However, the simultaneous delivery of O2 and pH-dependent anticancer drugs via nanomaterials and their effects on the viability of normal and cancer cells under hypoxic conditions have not been studied in sufficient numbers. This study describes the synthesis of a pH-responsive nanomaterial containing oxygen and anticancer drugs that exhibits sustained O2 release over a 14 d period under hypoxic conditions and pH-dependent sustained release of anticancer drugs over 30 d. The simultaneous administration of O2 and anticancer drugs results in higher cell survival of normal cells than that of cancer cells under hypoxic and normoxic conditions.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202400181","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141563676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modeling 3D Tumor Invasiveness to Modulate Macrophage Phenotype in a Human-Based Hydrogel Platform. 在人基水凝胶平台中模拟三维肿瘤侵袭性以调节巨噬细胞表型

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-06-28 DOI: 10.1002/mabi.202400227

Cátia F Monteiro, Catarina R Almeida, Catarina A Custódio, João F Mano

{"title":"Modeling 3D Tumor Invasiveness to Modulate Macrophage Phenotype in a Human-Based Hydrogel Platform.","authors":"Cátia F Monteiro, Catarina R Almeida, Catarina A Custódio, João F Mano","doi":"10.1002/mabi.202400227","DOIUrl":"10.1002/mabi.202400227","url":null,"abstract":"The immune system is a pivotal player in determining tumor fate, contributing to the immunosuppressive microenvironment that supports tumor progression. Considering the emergence of biomaterials as promising platforms to mimic the tumor microenvironment, human platelet lysate (PLMA)-based hydrogel beads are proposed as 3D platforms to recapitulate the tumor milieu and recreate the synergistic tumor-macrophage communication. Having characterized the biomaterial-mediated pro-regenerative macrophage phenotype, an osteosarcoma spheroid encapsulated into a PLMA hydrogel bead is explored to study macrophage immunomodulation through paracrine signaling. The culture of PLMA-Tumor beads on the top of a 2D monolayer of macrophages reveals that tumor cells triggered morphologic and metabolic adaptations in macrophages. The cytokine profile, coupled with the upregulation of gene and protein anti-inflammatory biomarkers clearly indicates macrophage polarization toward an M2-like phenotype. Moreover, the increased gene expression of chemokines identified as pro-tumoral environmental regulators suggest a tumor-associated macrophage phenotype, exclusively stimulated by tumor cells. This pro-tumoral microenvironment is also found to enhance tumor invasiveness ability and proliferation. Besides providing a robust in vitro immunomodulatory tumor model that faithfully recreates the tumor-macrophage interplay, this human-based platform has the potential to provide fundamental insights into immunosuppressive signaling and predict immune-targeted response.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing the Regenerative Potential of Purified Bovine Dental Pulp and Dentin Extracellular Matrices in a Chitosan/Alginate Hydrogel. 标题：利用壳聚糖/海藻酸盐水凝胶中纯化的牛牙髓和牙本质细胞外基质的再生潜力

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-06-27 DOI: 10.1002/mabi.202400254

Maree L Gould, Nerida J Downes, Adele G Woolley, Haizal M Hussaini, Jithendra T Ratnayake, Mohammad Azam Ali, Lara T Friedlander, Paul R Cooper

{"title":"Harnessing the Regenerative Potential of Purified Bovine Dental Pulp and Dentin Extracellular Matrices in a Chitosan/Alginate Hydrogel.","authors":"Maree L Gould, Nerida J Downes, Adele G Woolley, Haizal M Hussaini, Jithendra T Ratnayake, Mohammad Azam Ali, Lara T Friedlander, Paul R Cooper","doi":"10.1002/mabi.202400254","DOIUrl":"10.1002/mabi.202400254","url":null,"abstract":"When a tooth is diseased or damaged through caries, bioactive molecules are liberated from the pulp and dentin as part of the natural response to injury and these are key molecules for stimulating stem cell responses for tissue repair. Incorporation of these extracellular-matrix (ECM)-derived molecules into a hydrogel model can mimic in vivo conditions to enable dentin-pulp complex regeneration. Here, a chitosan/alginate (C/A) hydrogel is developed to sequester bovine ECM extracts. Human dental pulp cells (hDPCs) are cultured with these constructs and proliferation and cytotoxicity assays confirm that these C/A hydrogels are bioactive. Sequential z-axis fluorescent imaging visualizes hDPCs protruding into the hydrogel as it degraded. Alizarin red S staining shows that hDPCs cultured with the hydrogels display increased calcium-ion deposition, with dentin ECM stimulating the highest levels. Alkaline phosphatase activity is increased, as is expression of transforming growth factor-beta as demonstrated using immunocytochemistry. Directional analysis following phase contrast kinetic image capture demonstrates that both dentin and pulp ECM molecules act as chemoattractants for hDPCs. Data from this study demonstrate that purified ECM from dental pulp and dentin when delivered in a C/A hydrogel stimulates dental tissue repair processes in vitro.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic Effect of Multilayered Alginate/Poly(SBMA) Coatings on Marine Antifouling Property 多层海藻酸盐/聚（SBMA）涂料对海洋防污性能的协同效应

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-06-24 DOI: 10.1002/mabi.202400130

Jinwoo Lee, Inho Kim, Sung Min Kang

引用次数: 0

Mechanochemical Tailoring of Lignin Structure: Influence of Different Particle Sizes in the Organosolv Process 木质素结构的机械化学定制：有机溶胶工艺中不同颗粒尺寸的影响。

IF 4.4 4区医学

Macromolecular bioscience Pub Date : 2024-06-20 DOI: 10.1002/mabi.202400090

Jonas Bergrath, Franz Zeppetzauer, Jessica Rumpf, Birgit Kamm, Robert Putz, Hans-Willi Kling, Margit Schulze

{"title":"Mechanochemical Tailoring of Lignin Structure: Influence of Different Particle Sizes in the Organosolv Process","authors":"Jonas Bergrath, Franz Zeppetzauer, Jessica Rumpf, Birgit Kamm, Robert Putz, Hans-Willi Kling, Margit Schulze","doi":"10.1002/mabi.202400090","DOIUrl":"10.1002/mabi.202400090","url":null,"abstract":"The autocatalyzed ethanolic organosolv process is gaining increasing attention for the sulfur-free isolation of lignin, which is subsequently used as a renewable substitute for various fossil-based applications. For the first time, the mechanochemical influence of seven different particle sizes of two different biomasses on the respective organosolv lignin structure is examined. Wine pruning (Pinot Noir) and wine pomace (Accent) are used for organosolv process with particle sizes ranging from 2.0–1.6 mm to less than 0.25 mm. As particle size decreases, the weight-average molecular weight increases, while the total phenol content decreases significantly. Additionally, the distribution of the lignin-typical monolignols and relevant substructures, as determined by two-dimensional heteronuclear nuclear magnetic resonance spectra single quantum coherence (HSQC), is observed. The degree of grinding of the biomass has a clear chemical–structural influence on the isolated HG and HGS organosolv lignins. Therefore, it is crucial to understand this influence to apply organosolv lignins in a targeted manner. In the future, particle size specifications in the context of the organosolv process should be expressed in terms of distribution densities rather than in terms of a smaller than specification.","PeriodicalId":18103,"journal":{"name":"Macromolecular bioscience","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mabi.202400090","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141427166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0