Laboratory Animals最新文献_第5页

Monitoring Staphylococcus aureus nasal colonization murine model using a bioluminescent methicillin-resistant S. aureus (MRSA). 使用生物发光耐甲氧西林金黄色葡萄球菌（MRSA）监测金黄色葡萄球菌鼻腔定植小鼠模型。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-06-01 Epub Date: 2024-03-09 DOI: 10.1177/00236772231209790

Juliana G da Silva, Juliana Pc Boechat, Bruno Dj Silva, Rodrigo Müller, José Pm Senna

{"title":"Monitoring Staphylococcus aureus nasal colonization murine model using a bioluminescent methicillin-resistant S. aureus (MRSA).","authors":"Juliana G da Silva, Juliana Pc Boechat, Bruno Dj Silva, Rodrigo Müller, José Pm Senna","doi":"10.1177/00236772231209790","DOIUrl":"10.1177/00236772231209790","url":null,"abstract":"Staphylococcus aureus nasal carriage is considered a risk factor for infections, and the development of nasal decolonization strategies is highly relevant. Despite they are not naturally colonized by Staphylococcus, mice are a good model for S. aureus nasal colonization. Murine models are easy to manipulate, and inter-laboratory reproducibility makes them suitable for nasal colonization studies. Strategies using bioluminescent bacteria allow for the monitoring of infection over time without the need to sacrifice animals for bacterial quantification. In this study, we evaluated S. aureus nasal colonization in three mouse strains (BALB/c, C57BL/6, and Swiss Webster) using a bioluminescent strain (SAP231). In vitro, a visible Bioluminescent Signal Emission (BLSE) was observed until 106 bacteria and detected by IVIS® imaging system up to 104 cells. Animals were inoculated with one or two doses of approximately 109 colony-forming units (CFU) of SAP231. Swiss Webster mice showed the longest colonization time, with some animals presenting BLSE for up to 140 h. In addition, BLSE was higher in this strain. BALB/c and C57BL/6 strains showed consistent BLSE results for 48 h. BLSE intensity was higher in Swiss Webster inoculated with both doses. Three different positions for image capture were evaluated, with better results for the lateral and ventrodorsal positions. After the loss of BLSE, bacterial quantification was performed, and Swiss Webster mice presented more bacteria in the nasal cavity (approximately 105 CFU) than the other strains. Our results demonstrate that bioluminescent S. aureus allow monitoring of nasal colonization and estimation of the bacterial burden present in live animals until 48 h.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11264562/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140068534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combining melatonin with dexmedetomidine improves anesthesia in rats. 将褪黑素与右美托咪定结合使用可改善大鼠的麻醉效果。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-06-01 Epub Date: 2024-05-23 DOI: 10.1177/00236772231163211

Joaquín Cogo Pagella, María F Rodríguez, Fabricio Frisina, Claudio O Cervino

引用次数: 0

Pain management in zebrafish : Report from a FELASA Working Group. 斑马鱼的疼痛管理：FELASA 工作组的报告。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-06-01 Epub Date: 2023-12-05 DOI: 10.1177/00236772231198733

Lynne U Sneddon, Paul Schroeder, Ana Roque, Karin Finger-Baier, Angeleen Fleming, Simon Tinman, Bertrand Collet

{"title":"Pain management in zebrafish : Report from a FELASA Working Group.","authors":"Lynne U Sneddon, Paul Schroeder, Ana Roque, Karin Finger-Baier, Angeleen Fleming, Simon Tinman, Bertrand Collet","doi":"10.1177/00236772231198733","DOIUrl":"10.1177/00236772231198733","url":null,"abstract":"Empirical evidence suggests fishes meet the criteria for experiencing pain beyond a reasonable doubt and zebrafish are being increasingly used in studies of pain and nociception. Zebrafish are adopted across a wide range of experimental fields and their use is growing particularly in biomedical studies. Many laboratory procedures in zebrafish involve tissue damage and this may give rise to pain. Therefore, this FELASA Working Group reviewed the evidence for pain in zebrafish, the indicators used to assess pain and the impact of a range of drugs with pain-relieving properties. We report that there are several behavioural indicators that can be used to determine pain, including reduced activity, space use and distance travelled. Pain-relieving drugs prevent these responses, and we highlight the dose and administration route. To minimise or avoid pain, several refinements are suggested for common laboratory procedures. Finally, practical suggestions are made for the management and alleviation of pain in laboratory zebrafish, including recommendations for analgesia. Pain management is an important refinement in experimental animal use and so our report has the potential to improve zebrafish welfare during and after invasive procedures in laboratories across the globe.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11264547/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138487907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Urinary bladder catheterisation of female pigs: Influence of bladder content and Escherichia coli urinary tract infection on procedural outcome. 雌猪膀胱导尿术：膀胱内容物和大肠杆菌尿路感染对手术结果的影响。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-06-01 Epub Date: 2024-02-09 DOI: 10.1177/00236772231169344

Kristian Stærk, Louise Langhorn, Bo Halle, Thomas Emil Andersen

{"title":"Urinary bladder catheterisation of female pigs: Influence of bladder content and Escherichia coli urinary tract infection on procedural outcome.","authors":"Kristian Stærk, Louise Langhorn, Bo Halle, Thomas Emil Andersen","doi":"10.1177/00236772231169344","DOIUrl":"10.1177/00236772231169344","url":null,"abstract":"Catheterisation of the urinary bladder is needed in many types of human disease models in pigs. Based on our extensive experience with the pig as an infection model, we here demonstrate an approach of catheterising domestic pigs (40 attempts) and Göttingen minipigs (10 attempts) using a blinded method, that is, without speculums or videoscopes to visualise the urethral opening. The procedure was tested on control animals and pigs with experimental Escherichia coli urinary tract infection (UTI) to assess the potential influence of this condition on procedural outcome. Lastly, we performed cystoscopy in three animals to visualise the route to the urethra and to localise potential anatomical obstacles. All domestic pigs were catheterised successfully in an average of 2 minutes and 23 seconds, and this was not influenced by UTI (p = 0.06) or bladder urine content at the time of catheterisation (p = 0.32). All Göttingen minipigs were successfully catheterised in an average of 4 minutes and 27 seconds. We conclude that blinded catheterisation is a fast and reliable approach that can be performed in pigs with or without UTI with minimal risk of trauma or contamination.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139707116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bibliometric analysis on cannibalism/infanticide and maternal aggression towards pups in laboratory rodents. 关于实验室啮齿动物食人/杀婴行为和母性对幼崽攻击行为的文献计量分析。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-06-01 Epub Date: 2024-02-14 DOI: 10.1177/00236772231192030

José C Bravo, Lierni Ugartemendia, Arko Barman, Ana B Rodríguez, José A Pariente, Rafael Bravo

{"title":"Bibliometric analysis on cannibalism/infanticide and maternal aggression towards pups in laboratory rodents.","authors":"José C Bravo, Lierni Ugartemendia, Arko Barman, Ana B Rodríguez, José A Pariente, Rafael Bravo","doi":"10.1177/00236772231192030","DOIUrl":"10.1177/00236772231192030","url":null,"abstract":"Animal welfare has evolved during the past decades to improve not only the quality of life of laboratory rodents but also the quality and reproducibility of scientific investigations. Bibliometric analysis has become an important tool to complete the current knowledge with academic databases. Our objective was to investigate whether scientific research on cannibalism/infanticide is connected with maternal aggression towards the offspring in laboratory rodents. To carry out our research, we performed a specific search for published articles on each concept. Results were analyzed in the open-source environment RStudio with the package Bibliometrix. We obtained 253 and 134 articles for the first search (cannibalism/infanticide) and the second search (maternal aggression towards the pups) respectively. We observed that the interest in infanticide/cannibalism started in the 1950s, while researchers started showing interest in maternal aggression towards the pups 30 years later. Our analyses indicated that maternal aggression had better citations in scientific literature. In addition, although our results showed some common features (e.g. oxytocin or medial preoptic area in the brain), we observed a gap between cannibalism/infanticide and maternal aggression towards the pups with only 14 published articles in common for both the searches. Therefore, we recommend researchers to combine both concepts in further investigations in the context of cannibalism for better dissemination and higher impact in laboratory rodents' welfare research.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139729950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LAS around the globe. Balancing between bureaucracy and good science in Greece. 全球各地的 LAS。平衡希腊的官僚主义和良好科学。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2024-06-01 DOI: 10.1177/00236772231201076

Anastasia S Tsingotjidou

引用次数: 0

Thanks to Reviewers 感谢审稿人

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2024-05-18 DOI: 10.1177/00236772241235569

引用次数: 0

Comparing United States and European Union academic animal programs: Organization, operation, and services offered. 比较美国和欧盟学术动物项目：组织、运作和提供的服务。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2024-04-01 Epub Date: 2023-09-20 DOI: 10.1177/00236772231168925

Patricia Preisig, James D Macy, Jann Hau

{"title":"Comparing United States and European Union academic animal programs: Organization, operation, and services offered.","authors":"Patricia Preisig, James D Macy, Jann Hau","doi":"10.1177/00236772231168925","DOIUrl":"10.1177/00236772231168925","url":null,"abstract":"The Yale Animal Resource Cost and Benchmarking survey©, conducted in US academic Animal Research/Resource Centers (ARC), was modified to capture similar thematic information in European Union (EU; including the non-EU countries Switzerland and the UK) academic ARCs, which are members of the League of European Research Universities (LERU). Participating institutions came from Denmark, England, Finland, France, Germany, Ireland, Italy, Netherlands, Scotland, Spain, and Switzerland. Survey data analysis suggests that: (a) in LERU programs, it is common to have more than one ARC under the umbrella of a single institution with organizational \"lumping\" of the financial, regulatory, and/or operational tasks under one administrative unit; (b) accreditation by an outside agency (e.g., the Association for the Assessment and Accreditation of Laboratory Animal Care) is more common in US than LERU ARCs; (c) LERU ARCs are responsible for murine breeding, which contrasts with US ARCs, where ∼40% of rodent breeding is managed by researchers; (d) cryopreservation is the most frequently requested fee-for-service offering among LERU participants (75% of participants) compared to 30% of US participants; (e) like US programs, almost all LERU programs have mice and rats, but fewer LERU programs have nonhuman primates (NHPs), and none have dogs on census; (f) LERU ARCs have about an equal amount of vivarium housing and procedure space, while US facilities have twice as much housing as procedure space; (g) a higher percentage of LERU colonies are free of Helicobacter and murine norovirus compared to US colonies; and (h) more LERU ARCs used environmental microbiologic monitoring of rodent colonies compared to US programs.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41137405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving rat welfare through the development of a peribulbar anaesthesia technique for ophthalmic procedures: A preliminary study. 通过开发用于眼科手术的球周麻醉技术来改善大鼠的福利：一项初步研究。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2024-04-01 Epub Date: 2023-09-20 DOI: 10.1177/00236772231178433

Ady Eliav, Ron Ofri, Kelsey Brust, Yishai Kushnir, Yael Shilo-Benjamini

{"title":"Improving rat welfare through the development of a peribulbar anaesthesia technique for ophthalmic procedures: A preliminary study.","authors":"Ady Eliav, Ron Ofri, Kelsey Brust, Yishai Kushnir, Yael Shilo-Benjamini","doi":"10.1177/00236772231178433","DOIUrl":"10.1177/00236772231178433","url":null,"abstract":"Rats are a commonly used animal model for the study of the pathogenesis and novel treatments of glaucoma, which is induced experimentally using invasive, painful procedures. Peribulbar anaesthesia (PBA) is frequently used in people and domestic animals prior to ophthalmic surgeries to provide excellent perioperative analgesia. Our goal was to develop a PBA technique adapted to rat anatomy, improving the welfare of animals used as a model for glaucoma. Eighteen rat cadavers (n = 36 eyes) were used to establish the optimal needle insertion location. Five injection techniques using 0.1 mL/100 g lidocaine 2% and a contrast agent (1:1 volume ratio) were compared via computed tomography (CT). CT images were scored for injectate distribution at four locations: extraconal, intraconal, around the optic nerve and at the orbital fissure (scale 0-8, where 0 = none and 8 = excellent). Median scores using the dorso-medial-75° (5; range 2-6) and medial-canthus (4.5; range 2-8) injection techniques were not different from the dorso-medial-45° (4; range 3-6) technique and were higher (better distribution) compared with mid-ventral (3; range 2-5) and ventro-lateral (2; range 1-3) techniques. The two superior techniques were used in two experimental rats (n = 4 eyes) to determine the volume of bupivacaine 0.5% necessary to affect corneal touch threshold (CTT) and periocular skin sensitivity (PSS). A volume of 0.05 mL/100 g decreased CTT and PSS for several hours, while a larger volume produced excessively long effects. Dorso-medial-75° or medial-canthus PBA using 0.05 mL/100 g bupivacaine are likely to provide ocular and periocular analgesia in rats, with minor transient adverse effects.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41136861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rats prefer condensed milk to strawberry jam - a new possibility for voluntary oral drug administration. 大鼠更喜欢炼乳而不是草莓酱--自愿口服药物的一种新可能性。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2024-04-01 Epub Date: 2023-09-15 DOI: 10.1177/00236772231194389

Mariana Ferreira-Duarte, Igor Moreira Lopes, Manuela Morato, Margarida Duarte-Araújo

引用次数: 0