Laboratory Animals最新文献_第5页

Jacketed telemetry in rats: a novel non-invasive method for cardiorespiratory phenotyping during treadmill exercise. 大鼠夹套遥测：跑步机运动中心肺表型的新型无创方法。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-04-01 Epub Date: 2024-09-30 DOI: 10.1177/00236772241259857

Stéphane Tanguy, Agathe Cambier, Leandro Fontana-Pires, Timothé Flenet, Charles Eynard, Julie Fontecave-Jalon, Pierre-Yves Gumery, François Boucher

{"title":"Jacketed telemetry in rats: a novel non-invasive method for cardiorespiratory phenotyping during treadmill exercise.","authors":"Stéphane Tanguy, Agathe Cambier, Leandro Fontana-Pires, Timothé Flenet, Charles Eynard, Julie Fontecave-Jalon, Pierre-Yves Gumery, François Boucher","doi":"10.1177/00236772241259857","DOIUrl":"10.1177/00236772241259857","url":null,"abstract":"The development of alternative methods for monitoring cardiorespiratory function without restraint or surgical implantation is attracting growing interest for both ethical and scientific reasons. For this purpose, a new non-invasive jacketed telemetry tool consisting in a radio device maintained in a jacket worn by the animal was previously developed to improve cardiorespiratory monitoring. It allows simultaneous monitoring of cardiac activity by surface electrocardiagram, respiratory function by respiratory inductive plethysmography, and locomotor activity by accelerometry. However, this tool has only been validated under conditions of low/intermediate activity levels or in anesthetized animals. This study aimed to evaluate the feasibility of using this system in the challenging conditions of an exertion protocol. Male Wistar rats (n = 10, 8-9 weeks old) were subjected to an incremental treadmill exercise protocol including speed levels from 5 to 40 cm s-1 separated by 30-s breaks. Heart rate (HR) and minute ventilation (assessed by minute volume; MV) were continuously monitored. At the end of each running level and during the 30-s breaks, HR and MV showed a significant increase compared to resting values. They returned to the baseline within 60 min of post-exercise recovery. Overall, our results demonstrated (i) the ability of the animal to run while wearing the device and (ii) the ability of the device to reliably monitor cardiorespiratory adaptation to treadmill exercise despite significant mechanical disturbances. In conclusion, this study highlights the possibility of non-invasively monitoring cardiorespiratory functional variables that were previously unattainable under conditions of high activity in freely moving animals.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"215-225"},"PeriodicalIF":1.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preceding sevoflurane inhalation in rats reduces the stress of intraperitoneal injection both in inexperienced experimenters and subject rats. 在大鼠吸入七氟醚之前，可减少没有经验的实验者和受试大鼠腹腔注射时的应激反应。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-04-01 Epub Date: 2024-12-18 DOI: 10.1177/00236772241279680

Hidetoshi Ishibashi, Hideyuki Motohashi

引用次数: 0

Seasonality of the estrus cycle in laboratory mice under constant conditions. 恒定条件下实验室小鼠发情周期的季节性。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-03-30 DOI: 10.1177/00236772251318772

Tess M Reichard, Caitlin H Miller, Jay Yang, Michael J Sheehan

{"title":"Seasonality of the estrus cycle in laboratory mice under constant conditions.","authors":"Tess M Reichard, Caitlin H Miller, Jay Yang, Michael J Sheehan","doi":"10.1177/00236772251318772","DOIUrl":"https://doi.org/10.1177/00236772251318772","url":null,"abstract":"Seasonality governs every aspect of life in the natural environment. Controlled laboratory settings are intended to keep animals under a constant set of environmental cues with no seasonality. However, prior research suggests that seasonal variation may exist despite aseasonal lab environments. Here, we examined whether the length of each phase of the estrus cycle varied seasonally in addition to seasonal changes in the overall estrus cycle length in a laboratory mouse strain (C57BL/6J) under standard laboratory housing conditions. We found that female C57BL/6J mice exhibited reproductive seasonality mirroring the outside environment, in a controlled \"simulated summer\" environment. In the winter and spring, females have longer ovulating phases (proestrus and estrus), compared to the fall. Females similarly experience lengthier quiescent phases (metestrus and diestrus) in the summer, compared to fall and winter. Interestingly, females showed no significant variation in overall estrus cycle length across seasons. Notably, females spent more time in ovulating phases across seasons than previously reported. Laboratory mice are sensitive to external seasonal changes, even when housed in standard laboratory environments designed to control light, temperature, and humidity. Humidity is indicated by some analyses as a potential seasonal cue, however, we cannot rule out other unidentified external cues that may provide information about external seasonal changes. These findings represent just one example of how seasonality may impact mouse physiology in laboratory settings, emphasizing the need to account for such influences in biomedical research and improve environmental control in mouse holding facilities.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772251318772"},"PeriodicalIF":1.3,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An innovative minimally-invasive vaginoscopic approach for intrauterine infusion in rats - an infertility perspective. 一种创新的微创阴道镜方法用于大鼠宫内输注-不孕症的观点。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-03-17 DOI: 10.1177/00236772251317268

Azeena Saleem, Kalyanaraman Brindha, Alan M Punnoose, Radha Vembu

{"title":"An innovative minimally-invasive vaginoscopic approach for intrauterine infusion in rats - an infertility perspective.","authors":"Azeena Saleem, Kalyanaraman Brindha, Alan M Punnoose, Radha Vembu","doi":"10.1177/00236772251317268","DOIUrl":"https://doi.org/10.1177/00236772251317268","url":null,"abstract":"In fertility research, intrauterine administration in small animals presents significant technical challenges, often necessitating advanced and precise techniques. Historically, surgical methods have been preferred; however, these approaches are complex, invasive and expensive. While less invasive, intravaginal methods are generally performed without direct visualization and lack standardization, which raises the risk of complications and post-procedure mortality. We present a novel, minimally invasive technique that uses video-guided vaginoscopy to overcome these constraints. This technique efficiently eliminates the need for surgical intervention and improves safety and precision by enabling clear visualization and targeted delivery beyond the cervix. To facilitate the intrauterine delivery of agents, the method utilizes a modified 1 ml micropipette tip as a speculum, designed with a 5 mm wide slit as a technical aperture. The vaginoscope, a repurposed otoscope with an integrated camera and optimal focal length, was employed into the opposite end, which was linked to a mobile device enabling real-time visualization. This creative design reduced discomfort for the animal and the researcher while allowing for exact monitoring when the catheter entered the uterine lumen, guaranteeing precise speculum alignment and producing dependable and repeatable results. The protocol has been successfully implemented over 60 times, with all infusions achieving success and no adverse events reported. This minimally invasive intrauterine technique provides a straightforward, sustainable and effective method for delivering drugs or induction agents directly into the vaginal, cervical or uterine regions, making it suitable for applications in cell therapies, gene therapies and embryo transfers in assisted reproduction technologies.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772251317268"},"PeriodicalIF":1.3,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rodent surgeries: who is performing them and how? 啮齿动物手术：谁在做，怎么做？

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-03-13 DOI: 10.1177/00236772241297784

Felix Gantenbein, Sonja Hartnack, Stephan Zeiter, Charlotte Calvet, Petra Seebeck

{"title":"Rodent surgeries: who is performing them and how?","authors":"Felix Gantenbein, Sonja Hartnack, Stephan Zeiter, Charlotte Calvet, Petra Seebeck","doi":"10.1177/00236772241297784","DOIUrl":"https://doi.org/10.1177/00236772241297784","url":null,"abstract":"Surgery is an integral part of many experimental studies. Good surgical practice is a prerequisite for surgical success, optimal animal welfare, and it not only improves post-operative recovery but also the overall outcome and validity of a study. Rodents, especially mice, are the most commonly used laboratory animals and the legal requirements to perform experimental surgery are identical for all species. However, minimum surgical training requirements vary significantly across countries, ranging from basic introductory courses in animal experimentation to supervised, advanced courses led by expert surgeons; this complicates efforts toward standardization. This study provides insight into surgical education and experience, available infrastructure, workplace satisfaction, and the application of good surgical practice in laboratory rodent surgery. Two online surveys with a total of 72 questions were distributed across Europe and 782 complete responses were received and subsequently analyzed. The results showed that most researchers performing rodent surgery have no medical background. Furthermore, good surgical practice (i.e., sterile gowning and gloving, decontaminating and draping the patient, using sterile equipment) seems to be poorly implemented in rodent surgery. In addition, half of all rodent surgeons have no assistance available and most respondents expressed a desire for continued education and courses to deepen and refine their surgical skills. Consequently, training for rodent surgery should be tailored to the surgeon's preexisting knowledge, and additional surgical training should be made mandatory before performing surgery on laboratory rodents. This could improve both the animals' and the surgeons' welfare.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772241297784"},"PeriodicalIF":1.3,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of sex differences on the induction and evolution of clinical signs of an end-stage liver disease rat model. 性别差异对终末期肝病大鼠模型临床体征的诱导和进化的影响

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-03-13 DOI: 10.1177/00236772241309760

Ignacio Iborra, Ramon Bartoli, Ana Bargalló, Sergi Sunyé, Alba Ardèvol, Marta Fortuny, Sara Capdevila, Helena Masnou, Rosa M Morillas

{"title":"Impact of sex differences on the induction and evolution of clinical signs of an end-stage liver disease rat model.","authors":"Ignacio Iborra, Ramon Bartoli, Ana Bargalló, Sergi Sunyé, Alba Ardèvol, Marta Fortuny, Sara Capdevila, Helena Masnou, Rosa M Morillas","doi":"10.1177/00236772241309760","DOIUrl":"https://doi.org/10.1177/00236772241309760","url":null,"abstract":"BackgroundHistorically, preclinical studies with rat models have been carried out only with male animals. Current regulations require sex parity in experimental procedures. Several studies have shown significant sex differences in rat models of liver fibrosis, but there is no data available in end-stage liver disease. The aim was to describe sex-related differences in a carbon tetrachloride (CCl4)-induced rat model of cirrhosis with ascites.MethodsFifty-two rats, 26 of each sex, fed ad libitum with phenobarbital-enriched drinking water (5 mmol/l). CCl4 was administered orally weekly, adjusting doses to weight changes after CCl4 administration until ascites development.ResultsMedian time to ascites development was significantly higher in females (19 vs. 10 weeks). Males showed significantly greater weight changes 48 h after CCl4 administration. The cumulative dose of CCl4 was significantly higher in females, both at the time of diagnosis of ascites (10.7 vs. 1.5 ml) and at week 10 (median time to ascites development in males) (3.9 vs. 1.5 ml). There were no significant sex differences in model associated mortality (31% males vs. 27% females).ConclusionsSex differences have a significant impact on CCl4-induced end-stage liver disease; classical models should be redesigned to appropriately encompass both sexes.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772241309760"},"PeriodicalIF":1.3,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovative care protocol successfully rehabilitates non-human primates after MPTP-induced parkinsonism: Preliminary evidence from a restricted cohort of African Green Monkeys (Chlorocebus sabaeus). 创新的护理方案成功地恢复非人类灵长类动物在mptp诱导的帕金森病：来自非洲绿猴（绿猴）的初步证据。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-03-13 DOI: 10.1177/00236772241302576

Tamar Ravins Yaish, Noa Eshkol Noy, Rony Kalman, Jing Guang, Halen Baker Erdman, Orilia Ben-Yishay Nizri, Shimon Firman, Xiaowei Liu, Marc Deffains, Uri Werner-Reiss, Galith Abourbeh, Zvi Israel, Hagai Bergman, Lily Iskhakova

{"title":"Innovative care protocol successfully rehabilitates non-human primates after MPTP-induced parkinsonism: Preliminary evidence from a restricted cohort of African Green Monkeys (Chlorocebus sabaeus).","authors":"Tamar Ravins Yaish, Noa Eshkol Noy, Rony Kalman, Jing Guang, Halen Baker Erdman, Orilia Ben-Yishay Nizri, Shimon Firman, Xiaowei Liu, Marc Deffains, Uri Werner-Reiss, Galith Abourbeh, Zvi Israel, Hagai Bergman, Lily Iskhakova","doi":"10.1177/00236772241302576","DOIUrl":"https://doi.org/10.1177/00236772241302576","url":null,"abstract":"The MPTP-animal model of Parkinson's disease has significantly advanced our understanding of Parkinson's disease and the dopaminergic system, helping to establish disease mechanisms and develop therapeutic targets. The non-human primate (NHP) MPTP model is particularly valuable for replicating core Parkinson's disease motor symptoms, anatomical changes and electrophysiological variations seen in humans. However, MPTP-injection protocols often cause substantial suffering, leading to euthanasia. While some post-MPTP primates recovered spontaneously, purposefully induced recovery was considered unattainable. Our team developed a novel intensive care protocol (NICP) promoting complete recovery from MPTP-induced severe parkinsonism in NHPs. NICP provides therapeutic, nutritional and social support, enabling behavioral recovery and subsequent retirement to a primate sanctuary. This innovation enhances animal welfare and opens new prospects for veterinary care, emphasizing the need to explore recovery mechanisms for other chronic conditions induced for research.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"236772241302576"},"PeriodicalIF":1.3,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The FELASA 2025 Congress will take place in Athens, Greece, on 2-5 June 2025. FELASA 2025大会将于2025年6月2日至5日在希腊雅典举行。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-02-22 DOI: 10.1177/00236772251318087

Jean-Philippe Mocho

引用次数: 0

Development of a center for automated equipment processing to transform the research enterprise configuration. 建立自动化设备处理中心，改造科研企业配置。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-02-01 Epub Date: 2024-10-11 DOI: 10.1177/00236772241262827

Philip J Richter, Albert LeClair, Harry Lim

{"title":"Development of a center for automated equipment processing to transform the research enterprise configuration.","authors":"Philip J Richter, Albert LeClair, Harry Lim","doi":"10.1177/00236772241262827","DOIUrl":"10.1177/00236772241262827","url":null,"abstract":"The strategy of centralizing equipment sanitation and processing was developed by a top-tier public university to address the growing physical infrastructure and human resource challenges of its expanding in-vivo research enterprise. Subsequently, a center for automated equipment processing physically separate from all animal research facilities was developed and has operated consistently since 2016. The facility incorporates systems such as process automation to sanitize and sterilize equipment as efficiently as possible. Analysis of the differences between the new centralized and old distributed research enterprise configurations shows a total estimated fiscal benefit of almost US$54 m to date projected out to US$124 m through 2028. Utility consumption of operations over nine years in the new configuration was estimated to be decreased by over 125 million gallons of water, 14 million gallons of chilled water, 121 million pounds of steam, and almost 3.6 million kilowatts of electricity, with consumption savings significantly increased projected out to 2028. Additional operational and organizational benefits as well as direct research benefits were identified. The new configuration has functioned for nine years without detectable cross contamination within the research enterprise thus providing evidence that the location of equipment processing can be less of a \"microbial\" risk factor than previously attributed.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"23-33"},"PeriodicalIF":1.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142400684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Moving from guideline recommendations to practical, reasonable proposals: Biosafety considerations, breeding performance and challenges during specific pathogen-free animal facility enlargement. 从指南建议到实际、合理的建议：在特定的无病原体动物设施扩建期间的生物安全考虑、育种性能和挑战。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2025-02-01 Epub Date: 2025-01-24 DOI: 10.1177/00236772241301123

Maschi Fabricio, Laborde Juan, Carriquiriborde Martín, Milocco Silvana, Almirón Johana, Resasco Agustina, Ayala Miguel

{"title":"Moving from guideline recommendations to practical, reasonable proposals: Biosafety considerations, breeding performance and challenges during specific pathogen-free animal facility enlargement.","authors":"Maschi Fabricio, Laborde Juan, Carriquiriborde Martín, Milocco Silvana, Almirón Johana, Resasco Agustina, Ayala Miguel","doi":"10.1177/00236772241301123","DOIUrl":"10.1177/00236772241301123","url":null,"abstract":"Extending an existing animal facility is a challenging process that requires consideration of both engineering and biological aspects. In this sense, integration with ongoing activities must not alter the animals' microbiological condition or welfare, as they usually remain in the facility while these activities occur. The objective of this work was to describe and evaluate the practical biosafety considerations during the enlargement of a specific pathogen-free (SPF) rodent facility. Our facility breeds rats and mice free of a list of zoonotic and common rodent pathogens, comprising 6 ectoparasites, 13 endoparasites, 25 bacteria and 23 viruses. In this project, the new SPF area was connected to an old but still working SPF rodent facility through the original clean corridor. The old clean corridor remained sealed throughout the project, and it was not opened until the new area was finished and fully equipped, all the new rooms were cleaned and disinfected, and the environment was evaluated for the presence of pathogens. Timing during the project was essential, as avoidance of the period of high animal production and demand was sought. The microbiological controls showed no growth of microorganisms in any new room. Thus, the applied procedures were deemed effective. It was concluded that protocols should be carefully planned in order to maintain the SPF condition and animal welfare.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"75-82"},"PeriodicalIF":1.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143033475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0