Laboratory Animals最新文献_第5页

How to teach practical skills with live laboratory animals? Using collective reflection to explore approaches and systematise strategies. 如何教授活体实验动物的实践技能？利用集体反思探索方法和系统化策略。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-04-01 Epub Date: 2026-03-11 DOI: 10.1177/00236772251407875

Andreia Costa, Júlio Borlido Santos, I Anna S Olsson

{"title":"How to teach practical skills with live laboratory animals? Using collective reflection to explore approaches and systematise strategies.","authors":"Andreia Costa, Júlio Borlido Santos, I Anna S Olsson","doi":"10.1177/00236772251407875","DOIUrl":"10.1177/00236772251407875","url":null,"abstract":"Researchers need training to develop adequate competence before using animals in experimental procedures. In contrast to medical training, there has been almost no scholarly attention to questions of methodology for teaching practical skills with laboratory animals. In this paper, we explore how the experience of laboratory animal trainers can be combined with existing scholarship on learning and teaching practical skills in related fields. Data were collected through facilitated group discussions in a series of workshops involving a total of 72 participants from 18 countries. Participants identified the 'See one, Do one' method as their preferred teaching approach in practical classes, and recognised the importance of strategies for progressive and adapted learning. They also acknowledged different types of challenging situations, including trainees who have previously developed proficiency with incorrect techniques, trainees who appear uninterested, trainees expressing aversion to animals and trainees who are reluctant to subject animals to harmful procedures. Although participants presented strategies for overcoming the majority of these challenges, they also highlighted specific scenarios where they felt unable to provide adequate support or overcome the challenges within the training environment. This work represents a first systematic scholarly effort to capture and describe teaching strategies used by practical laboratory animal science trainers, an essential step for education and competency development research and practice in the field.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"205-214"},"PeriodicalIF":1.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147434234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcranial direct current stimulation (tDCS) in conscious mice: a refined method for repetitive applications without restraint. 有意识小鼠的经颅直流电刺激（tDCS）：一种无约束重复应用的改进方法。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-04-01 Epub Date: 2025-12-14 DOI: 10.1177/00236772251391978

Nora Rautenberg, Stefan Johannes Blaschke, Susan Vlachakis, Michael Schroeter, Gereon Rudolf Fink, Maria Adele Rueger

{"title":"Transcranial direct current stimulation (tDCS) in conscious mice: a refined method for repetitive applications without restraint.","authors":"Nora Rautenberg, Stefan Johannes Blaschke, Susan Vlachakis, Michael Schroeter, Gereon Rudolf Fink, Maria Adele Rueger","doi":"10.1177/00236772251391978","DOIUrl":"10.1177/00236772251391978","url":null,"abstract":"Transcranial direct current stimulation (tDCS) for painless, non-invasive brain stimulation is a promising approach to enhance recovery after stroke. However, the precise molecular and cellular mechanisms underlying the effects of tDCS remain unclear, impeding its use in humans and necessitating research in experimental animals. Unlike humans, who receive tDCS fully awake, experimental models of tDCS have so far been conducted under anaesthesia to ensure the immobility required by currently available experimental setups. The use of anaesthesia may, however, confound results, decreasing their translational value. To address this problem, we developed a refined method enabling tDCS in awake and freely moving mice. A tube attached to the skull contains the stimulation electrode, whose position can be adapted flexibly to the target brain region; the reference electrode is implanted subcutaneously at the contralateral chest. Here, we report n = 135 awake mice that underwent tDCS in their home cage for up to 15 minutes per session over 10 consecutive days. None of those animals showed any adverse effects, neither regarding their general wellbeing, body weight, and behavioural activity, nor in ex vivo histology and immunohistochemistry. We suggest using this refined approach in future experimental studies of neuropsychological disorders.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"157-170"},"PeriodicalIF":1.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145757061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Darkness during behavioral tests induces exploratory activity and decreases anxiety-related behavior in male mice. 行为测试期间的黑暗诱导了雄性小鼠的探索活动并减少了与焦虑相关的行为。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-04-01 Epub Date: 2026-03-08 DOI: 10.1177/00236772261418962

Kseniya Avimova, Dmitry Sandakov

{"title":"Darkness during behavioral tests induces exploratory activity and decreases anxiety-related behavior in male mice.","authors":"Kseniya Avimova, Dmitry Sandakov","doi":"10.1177/00236772261418962","DOIUrl":"10.1177/00236772261418962","url":null,"abstract":"Light is presumed to be highly aversive to laboratory rodents. It can change their physiological responses and activity. Much behavioral experimentation is conducted under dim light, though even dim light may alter the behavior of animals. Some research facilities use a reversed light regimen and provide darkness during routine and experimental procedures. However, there are no unified guidelines of light intensity during behavioral testing, and \"dim light\" is being understood very broadly. It can be suggested that testing in darkness can be ethologically relevant for mice, rats, and other rodents who are known for nocturnality in laboratory conditions. We hypothesized that laboratory mice would be more active when being tested in a dark room than in an illuminated one regardless of the circadian phase. To test our hypotheses we conducted an experiment to evaluate male outbred mice behavior in four standard tests widely used in preclinical studies: the open field test, the hole-board test, the tail suspension test, and the elevated plus maze test. Locomotion in the open field test increased in darkness. The hole-board test revealed significantly reduced exploratory activity when performed in a dark room. In the elevated plus maze, mice tested in darkness behave less anxiously than those tested in light. Illumination did not affect mobility of mice in the tail suspension test. We suppose that darkness reduces anxiety and, hence, induces locomotion and exploration in mice in tests that are provided to study these traits. The tail suspension test is aversive itself, so it may mask any environmental effects.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"171-181"},"PeriodicalIF":1.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147378101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Implications of stress for animal welfare and animal-based research^†. 应激对动物福利和动物基础研究的影响。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-04-01 Epub Date: 2025-12-14 DOI: 10.1177/00236772251396310

Sarah E Wolfensohn, Vera Baumans, F Josef van der Staay, Saskia S Arndt

{"title":"Implications of stress for animal welfare and animal-based research†.","authors":"Sarah E Wolfensohn, Vera Baumans, F Josef van der Staay, Saskia S Arndt","doi":"10.1177/00236772251396310","DOIUrl":"10.1177/00236772251396310","url":null,"abstract":"Better animal welfare is essential for better animal-based science. Poor welfare induces stress (and vice versa), which in turn can affect or even severely confound research results. To ensure the validity of scientific results, those who work with laboratory animals must take responsibility for their care and welfare. A harm-benefit analysis can be used to weigh the welfare and quality of life of animals in scientific studies against the resulting benefits to other animals or humans, provided that animal welfare can be validly assessed. This review considers the identification and characterisation of stress by physiological, hormonal, immunological and behavioural measures and by assessment of the physical condition of the animal. It addresses controllability, predictability, chronicity, duration and severity, and the intrinsic and extrinsic factors that modulate animal behaviour and coping mechanisms in response to stress. EU Directive 2010/63 requires procedures to be classified as mild, moderate or severe. However, as long as some researchers use purely subjective assessments without a supporting structure and reference scales, or no assessment method at all, these terms may have limited practical value. The challenge is to understand the state of the animal from the information available. Welfare assessment tools can be used to demonstrate the true impact of research procedures and their refinement to protect animal welfare. If any doubt exists about the harm-benefit evaluation of the experiment then the welfare of the animal should take priority.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"144-156"},"PeriodicalIF":1.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13187226/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145756986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

News from the EIC team. 来自EIC团队的消息。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-04-01 Epub Date: 2026-05-04 DOI: 10.1177/00236772261445336

Fernando Gonzalez-Uarquin, Paulin Jirkof, Annemarie Lang, Jordi L Tremoleda

引用次数: 0

Research sense: Incorporating animals' sensory capacities in animal care and study design. 研究意识：将动物的感官能力融入动物护理和研究设计中。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-04-01 Epub Date: 2025-12-14 DOI: 10.1177/00236772251385784

Mal Graham, Bob Fischer

引用次数: 0

Evaluation of the convergence site as a refinement over the facial vein for chin bleed of CD1 female mice. CD1雌性小鼠颏出血会聚部位的评价。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-04-01 Epub Date: 2026-03-11 DOI: 10.1177/00236772251411794

Andrea Pardo-Pedraza, Chereen Collymore, Lucia Amendola

引用次数: 0

A review of the comparative anatomy of the domestic pig (Sus scrofa domestica) and its relevance to translational research in abdominal transplantation. 综述了家猪（Sus scrofa domestica）的比较解剖学及其与腹部移植转化研究的相关性。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-04-01 Epub Date: 2026-01-23 DOI: 10.1177/00236772251397586

Dylan R Barnett, Rohan Bhattacharjya, Shantanu Bhattacharjya

{"title":"A review of the comparative anatomy of the domestic pig (Sus scrofa domestica) and its relevance to translational research in abdominal transplantation.","authors":"Dylan R Barnett, Rohan Bhattacharjya, Shantanu Bhattacharjya","doi":"10.1177/00236772251397586","DOIUrl":"10.1177/00236772251397586","url":null,"abstract":"This narrative review summarizes and synthesizes the current literature regarding the comparative anatomy of pigs and humans, focusing on organ systems commonly involved in abdominal transplantation with the aim of supporting the safe and effective use of porcine models in preclinical studies. It also integrates practical insights from experimental surgery to highlight key considerations in perioperative care and surgical technique in porcine models. Pigs demonstrate substantial anatomical and physiological similarities to humans, making them ideal large-animal models for transplantation research. The recent surge in reports concerning clinical porcine xenotransplantation underscores the increasing importance of comprehending this comparative anatomy in the forthcoming years. However, critical species-specific anatomical differences particularly involving the gastrointestinal tract, hepatobiliary system, genitourinary tract and vascular structures require careful understanding to avoid surgical complications. Proper handling, perioperative care and awareness of porcine-specific stress responses and arrhythmias susceptibility are also essential to prevent premature animal loss. A thorough understanding therefore of porcine comparative anatomy is essential for transplant researchers conducting large animal surgery. Such knowledge improves experimental outcomes, supports adherence to the 3Rs (Replacement, Reduction and Refinement) and enhances the translational value of porcine models in transplantation research.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"131-143"},"PeriodicalIF":1.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146030373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Widespread antimicrobial use in laboratory rodent vivaria in North America revealed by a cross-sectional survey of American College of Laboratory Animal Medicine certified veterinarians. 美国实验动物医学学院认证兽医的横断面调查显示，北美实验室啮齿动物体内广泛使用抗菌药物。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-04-01 Epub Date: 2025-12-11 DOI: 10.1177/00236772251385020

Rebbecca S Wilcox, Marc S Marenda, Joanne M Devlin, Kathleen R Pritchett-Corning, Colin R Wilks

{"title":"Widespread antimicrobial use in laboratory rodent vivaria in North America revealed by a cross-sectional survey of American College of Laboratory Animal Medicine certified veterinarians.","authors":"Rebbecca S Wilcox, Marc S Marenda, Joanne M Devlin, Kathleen R Pritchett-Corning, Colin R Wilks","doi":"10.1177/00236772251385020","DOIUrl":"10.1177/00236772251385020","url":null,"abstract":"Antimicrobial resistance (AMR) is a major global threat to human health, animal welfare and sustainable development. Whilst resistance can arise naturally, inappropriate antimicrobial exposure accelerates its emergence. Recognizing this risk, international One Health initiatives emphasize reducing antimicrobial use, especially of drugs considered critical for human medicine. Much of the evidence guiding these efforts comes from food-producing and companion animals, but antimicrobial use in laboratory animals, particularly rodents, remains poorly described. To address this gap, we surveyed all veterinarians certified by the American College of Laboratory Animal Medicine in the United States (921) and Canada (10). A total of 157 veterinarians completed the survey, resulting in a 16.9% overall response rate. The response rate was 16.6% for participants in the United States and 40.0% for those in Canada. Despite a strong response, the small number of eligible veterinarians restricts data extrapolation in the Canadian context. Survey results showed that antimicrobial use is common and routine in rodent vivaria. Overall, 91.7% of respondents reported regular antimicrobial use. Applications included both clinical treatment and research purposes such as induction of microbiome dysbiosis, prophylaxis in immunocompromised rodents and gene induction. Reported drug classes included fluoroquinolones and third-generation cephalosporins, with some use of glycopeptides and carbapenems, all considered critically important to human health. Disposal practices often involved release into sewerage or landfill without inactivation, raising environmental concerns. These findings suggest that antimicrobial use in laboratory rodents may represent an underrecognized contributor to AMR and highlight the importance of targeted stewardship.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":" ","pages":"193-204"},"PeriodicalIF":1.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145743392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sixty Years of Laboratory Animals. 实验动物的六十年。

IF 1.3 4区农林科学

Laboratory Animals Pub Date : 2026-02-01 Epub Date: 2026-03-31 DOI: 10.1177/00236772261421513

Jan-Bas Prins

引用次数: 0