Laboratory Animals最新文献_第10页

Announcement of the V SPCAL Congress. 第五届SPCAL大会的公告。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2023-10-01 DOI: 10.1177/00236772231197935

M J Castelhano-Carlos

引用次数: 0

Willkommen zur 60. Wissenschaftlichen Tagung der Gesellschaft für Versuchstierkunde GV-SOLAS und 21. Fortbildungsveranstaltung der IGTP 6. - 8. September 2023, Mainz, Deutschland. 欢迎来到60年代。实验动物科学学会科学会议GV-SOLAS和21。IGTP 6培训活动-2023年9月8日，德国美因茨。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2023-08-01 DOI: 10.1177/00236772231185978

{"title":"Willkommen zur 60. Wissenschaftlichen Tagung der Gesellschaft für Versuchstierkunde GV-SOLAS und 21. Fortbildungsveranstaltung der IGTP 6. - 8. September 2023, Mainz, Deutschland.","authors":"","doi":"10.1177/00236772231185978","DOIUrl":"10.1177/00236772231185978","url":null,"abstract":"Nach nunmehr 5 Jahren findet die Jahrestagung der Gesellschaft für Versuchstierkunde GV-SOLAS erstmals wieder in Pr€asenz statt. Wir freuen uns, Sie in der sch€ onen Stadt Mainz am Rhein vom 06.-08.09.2023 begrüßen zu dürfen. Es erwarten Sie interessante Workshops, Diskussionsrunden, Vortr€age und Poster zu aktuellen Themen der Versuchstierkunde. Gleichzeitig werden wir auch wissenschaftliche Beitr€age anderer Fachrichtungen der experimentellen Forschung einbinden. Denn die Kernfrage jeder Forschung bleibt – “Warum und wofür ben€ otigen wir weiterhin Versuchstiere?” Ein weiteres Schwerpunktthema besch€aftigt sich mit dem immer wieder aktuellen 3R-Prinzip. Hierbei erwarten wir spannende Beitr€age rund um den Tierschutz unserer Versuchstiere aber auch interessante Neuigkeiten alternativer Ans€atze. Im Thema “Culture of Care – Compassion fatigue” soll der Blick auf die pers€ onliche Kultur der Fürsorge und Verantwortung gerichtet werden. Ebenfalls von großem Interesse ist auch immer mehr das Thema “Recht”. Besonders die Umsetzungen der Novellierungen des deutschen Tierschutzgesetzes und der Tierschutzversuchstierverordnung werfen ungeahnte Probleme auf. Hier lohnt es sich, mehr zu diesem Thema zu h€ oren und Erfahrungen auszutauschen. Auch das Thema Zuchtplanung und die Verwendung von “überz€ahligen Tieren” wird auf dieser Tagung eine große Rolle spielen. Weitere geplante Schwerpunktthemen für die Jubil€aumstagung befassen sich mit An€asthesie und Analgesie sowie Hygiene und deren Einfluss auf das Mikrobiom, oder haben Tierversuche mit landwirtschaftlichen Tieren, Wildtieren oder Exoten im Fokus. Für Kollegen aus dem Inund Ausland bieten wir zus€atzlich die M€ oglichkeit, viele Vortr€age in den Haupts€alen online verfolgen zu k€ onnen und sich rege an den anschließenden Diskussionen zu beteiligen. Weitere Informationen finden Sie unter https:// www.gv-solas2023.de/ Wir freuen uns auf Sie!","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10172376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Automatic measurement of mouse visual acuity based on optomotor response: SKY optomotry. 基于视运动反应的小鼠视敏度自动测量:SKY视光。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2023-08-01 DOI: 10.1177/00236772221148576

So Yeon Ahn, Eun Hye Jung, Hyunmin Ahn, Jihei Sara Lee, Jeong Hyeon Bak, Eun-do Kim, Ja-Hyun Song, Hae-Sol Shin, Munkhdelger Jamiyansharav, Kyoung Yul Seo

{"title":"Automatic measurement of mouse visual acuity based on optomotor response: SKY optomotry.","authors":"So Yeon Ahn, Eun Hye Jung, Hyunmin Ahn, Jihei Sara Lee, Jeong Hyeon Bak, Eun-do Kim, Ja-Hyun Song, Hae-Sol Shin, Munkhdelger Jamiyansharav, Kyoung Yul Seo","doi":"10.1177/00236772221148576","DOIUrl":"https://doi.org/10.1177/00236772221148576","url":null,"abstract":"In the field of visual science study using rodents, several assessment methods have been developed for measuring visual function. However, methods such as electroretinograms tests, visual evoked potentials tests and maze tests have limitations in that they measure function of only a specific type of cells, are difficult to quantify or require sufficient training time. The method which uses an optokinetic reflex and optomotor response, a compensatory eye and head movement in response to changes in the visual scene, became the most widely used method. However, this method requires highly trained experimenters and is time consuming. We showed that measured visual acuity values are significantly different between beginner and expert. Here we suggest an automated optometry program, 'SKY optomotry', which automatically tracks rodents' optomotor response to overcome subjectivity and the lengthy scoring procedure of the existing method. To evaluate the performance of SKY optomotry using 8-12-week-old C57BL/6 mice we compared the binomial decision of SKY optomotry with a skilled expert, and the area under the curve of SKY optomotry was 0.845. Comparing the final visual acuity, the intraclass correlation coefficient value between SKY optomotry and an expert was 0.860 (95% confidence interval (CI) 0.709-0.928), whereas that between an expert and a beginner was 0.642 (95% CI 0.292-0.811). SKY optomotry showed an excellent level of performance with good inter-rater agreements based on the visual acuity measured by an expert. With the use of our application, researchers will be able to test an experimental animal's eyesight more accurately while saving time on specialized training.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10536329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scoring the Culture of Care as a key performance indicator in a global pharmaceutical company. 将关怀文化作为一家全球制药公司的关键绩效指标进行评分。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2023-08-01 DOI: 10.1177/00236772231151516

Océane Turkménian, Aurore Rocua, Thierry Decelle

{"title":"Scoring the Culture of Care as a key performance indicator in a global pharmaceutical company.","authors":"Océane Turkménian, Aurore Rocua, Thierry Decelle","doi":"10.1177/00236772231151516","DOIUrl":"https://doi.org/10.1177/00236772231151516","url":null,"abstract":"Culture of Care has been an integral part of Sanofi's policy on animal protection for more than 15 years. Culture of Care goes beyond compliance with regulations and standards, since it relates to the attitudes of staff and to caring for research animals, as well as to the company's commitment to an active animal protection policy. However, the concept of Culture of Care remains subjective. Via two anonymous and voluntary surveys conducted in 2018 and 2021, it was possible to understand the level of staff engagement and the perception of company efficiency. Based on key questions, it was possible to assess individual engagement (animal welfare knowledge, pride, engagement, recognition) and company commitments (oversight body efficiency, level of transparency, Sanofi policy on animal protection). The institutional scores were 7.7/10 and 7.9/10 obtained in 2018 and in 2021, respectively. The individual score obtained for 2018 was 6.7/10 compared to 6.9/10 obtained in 2021. The combination of these two criteria helps to determine a Culture of Care score and thus make it a performance indicator. The scores are CoC2018 (7.7; 6.7) and CoC2021 (7.9; 6.9). Being able to quantify this level of engagement and the perception that employees have of the company encourages the organisation of an improvement programme and helps measure the benefits.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10164178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Nomenclature for standardized designation of diploid genotypes in genetically modified laboratory animals. 转基因实验动物二倍体基因型标准化命名的命名法。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2023-08-01 DOI: 10.1177/00236772231175727

Peter Dobrowolski, Thorsten Buch, Stefan Nagel-Riedasch

{"title":"Nomenclature for standardized designation of diploid genotypes in genetically modified laboratory animals.","authors":"Peter Dobrowolski, Thorsten Buch, Stefan Nagel-Riedasch","doi":"10.1177/00236772231175727","DOIUrl":"10.1177/00236772231175727","url":null,"abstract":"Information about the diploid genotype of a gene-modified or mutant laboratory animal is essential for breeding and experimental planning. It is also required for the exchange of animals between different research groups or for communication with professional genotyping service providers. While there are detailed, standardized rules for creating an allele name of a genome modification or mutation, the notation of the diploid genotype after biopsy and genotyping has not been standardized yet. Therefore, a uniform, generally understandable nomenclature for the diploid genotype of gene-modified laboratory animals is needed. With the here-proposed nomenclature recommendations from the Committee on Genetics and Breeding of Laboratory Animals of the German Society for Laboratory Animal Science (GV-SOLAS), we provide a practical, standardized representation of the genotype of gene-modified animals. It is intended to serve as a compact guide for animal care and scientific personnel in animal research facilities and to simplify data exchange between groups and with external service providers.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10506360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10307243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Psychological stress and strain in laboratory animal professionals - a systematic review. 实验动物专业人员的心理压力与紧张——系统综述。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2023-08-01 DOI: 10.1177/00236772221129111

Sonja Rumpel, Regina Kempen, Roswitha Merle, Christa Thoene-Reineke

{"title":"Psychological stress and strain in laboratory animal professionals - a systematic review.","authors":"Sonja Rumpel, Regina Kempen, Roswitha Merle, Christa Thoene-Reineke","doi":"10.1177/00236772221129111","DOIUrl":"https://doi.org/10.1177/00236772221129111","url":null,"abstract":"Laboratory animal professionals (LAP) are faced with various situations and tasks influencing their mental well-being. A systematic review has been conducted to investigate whether there are specific stressors for LAP and which moderators are relevant for the development of psychological strain. A comprehensive search following PRISMA Guidelines was carried out in June 2021. Results include 12 studies and have been summarized qualitatively in narrative synthesis and tabular presentation. Available literature indicates that LAP are facing stressors but does not allow for conclusions on specific stressful job duties other than euthanasia.Signs of strain are present in LAP. Specifically, participants in qualitative studies reported acute symptoms, while chronic manifestations were in focus in quantitative studies. Although a wide variety of moderating factors have been investigated, only social support has been rated as relevant by multiple qualitative and quantitative studies without contrasting results.According to current data, there is a risk for psychological strain in LAP. However, there is limited understanding of specific stressors and data on moderators is diverse. Further studies that focus on domain-specific knowledge and clearly distinguish stressors from moderators are necessary to set up institutional programmes addressing psychological strain in LAP.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10155638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Media training: 'Communicating your animal research', SECAL-EARA. 媒体培训：“交流你的动物研究”，SECAL-ERA。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2023-08-01 DOI: 10.1177/00236772231185231

引用次数: 0

Comparison of unilateral and bilateral embryo transfer in mice. 小鼠单侧和双侧胚胎移植的比较。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2023-08-01 DOI: 10.1177/00236772221149844

Kerstin E Auer, Thomas Kolbe, Claudia Laschalt, Thomas Rülicke

{"title":"Comparison of unilateral and bilateral embryo transfer in mice.","authors":"Kerstin E Auer, Thomas Kolbe, Claudia Laschalt, Thomas Rülicke","doi":"10.1177/00236772221149844","DOIUrl":"https://doi.org/10.1177/00236772221149844","url":null,"abstract":"Surgical embryo transfer in mice is a key technique in assisted reproduction and applied for different purposes in biomedical research. Due to its frequent application in rodent facilities across the world, further improvement of the procedure can substantially contribute to fulfil the principles of the 3Rs. Here, we investigated the effect of bilateral and unilateral left- or right-sided oviduct transfers on the success of embryo transfers. In total, we performed 223 embryo transfers (56 unilateral left, 56 unilateral right, 111 bilateral), in which we transferred 10-14 two-cell embryos each. We found that the type of transfer significantly influenced both the pregnancy rate of recipients and the survival rate of transferred embryos. Bilateral transfers yielded higher pregnancy and survival rates than left-sided unilateral transfers. Right-sided unilateral transfers yielded higher pregnancy rates than left-sided unilateral transfers and did not differ in embryo survival rates from bilateral transfers. We found no evidence that the number of transferred embryos affected the pregnancy rate. However, the number of born pups increased with the number of transferred embryos. In conclusion, unilateral embryo transfers into the right reproductive tract yield equally high pregnancy and embryo survival rates as bilateral transfers. Given that a second abdominal incision can be prevented and the time of surgery can be reduced, we recommend applying unilateral right-sided transfers, as this would reduce postoperative pain and lower the impact on recipients.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10155650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

When less is more: Experimental Bishop-Koop technique for reduction in the use of laboratory animals for intestinal pathophysiological studies. 少即是多:实验性Bishop-Koop技术，用于减少肠道病理生理研究中实验动物的使用。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2023-08-01 DOI: 10.1177/00236772231151563

Juan Cruz Abate, Natalia Lausada, Leandro Vecchio Dezillio, Jeremías Moreira, Ivana Ivanoff Marinoff, Maria Malena Ferreyra Compagnucci, Ane Miren Andrés Moreno, Carlota Largo, Martín Rumbo, Francisco Hernández Oliveros, David Romanin, Pablo Stringa

{"title":"When less is more: Experimental Bishop-Koop technique for reduction in the use of laboratory animals for intestinal pathophysiological studies.","authors":"Juan Cruz Abate, Natalia Lausada, Leandro Vecchio Dezillio, Jeremías Moreira, Ivana Ivanoff Marinoff, Maria Malena Ferreyra Compagnucci, Ane Miren Andrés Moreno, Carlota Largo, Martín Rumbo, Francisco Hernández Oliveros, David Romanin, Pablo Stringa","doi":"10.1177/00236772231151563","DOIUrl":"https://doi.org/10.1177/00236772231151563","url":null,"abstract":"The use of animals to gain knowledge and understanding of diseases needs to be reduced and refined. In the field of intestinal research, because of the complexity of the gut immune system, living models testing is mandatory. Based on the 3Rs (replacement, reduction and refinement) principles, we aimed to developed and apply the derived-intestinal surgical procedure described by Bishop and Koop (BK) in rats to refine experimental gastrointestinal procedures and reduce the number of animals used for research employing two models of intestinal inflammation: intestinal ischemia-reperfusion injury and chemical-induced colitis. Our results show the feasibility of the application of the BK technique in rodents, with good success after surgical procedure in both small and large intestine (100% survival, clinical recovery and weight regain). A considerable reduction in the use of the number of rats in both intestinal inflammation models (80% in case of intestinal ischemia-reperfusion damage and 66.6% in chemical-induced colitis in our experimental design) was achieved. Compared with conventional experimental models described by various research groups, we report excellent reproducibility of intestinal damage and functionality, survival rate and clinical status of the animals when BK is applied.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10518304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The human skin organ culture model as an optimal complementary tool for murine pemphigus models. 人皮肤器官培养模型作为小鼠天疱疮模型的最佳补充工具。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2023-08-01 DOI: 10.1177/00236772221145647

Veronika Hartmann, William Vj Hariton, Siavash Rahimi, Christoph M Hammers, Ralf J Ludwig, Eliane J Müller, Jennifer E Hundt

{"title":"The human skin organ culture model as an optimal complementary tool for murine pemphigus models.","authors":"Veronika Hartmann, William Vj Hariton, Siavash Rahimi, Christoph M Hammers, Ralf J Ludwig, Eliane J Müller, Jennifer E Hundt","doi":"10.1177/00236772221145647","DOIUrl":"https://doi.org/10.1177/00236772221145647","url":null,"abstract":"Pemphigus is a severe autoimmune bullous disease of the skin and/or mucous membranes caused by autoantibodies that mainly target the adhesion proteins desmoglein (Dsg) 3 and/or Dsg1. Clinically, pemphigus is characterized by flaccid blistering, leading to severe water and electrolyte loss. Before the introduction of corticosteroid treatment, the disease turned out to be fatal in many cases. Despite recent therapeutic improvements, treatment of pemphigus patients is centred on prolonged systemic immunosuppression and remains challenging. Current drug development for pemphigus has a strong focus on disease-causing B cells and autoantibodies and, more recently, also on modulating autoantibody-induced tissue pathology and keratinocyte signalling. This drug development requires reliable pre-clinical model systems replicating the pathogenesis of the human disease. Among those are neonatal and adult mouse models based on the transfer of Dsg3, Dsg1/3 or Dsg1-specific autoantibodies. To reduce the number of animal experiments, we recently established a standardized human skin organ culture (HSOC) model for pemphigus. This model reproduces the clinical phenotype of autoantibody-induced tissue pathology in pemphigus vulgaris. For induction of blistering, a recombinant single-chain variable fragment (scFv) targeting both Dsg1 and 3 is injected into pieces of human skin (obtained from plastic surgeries). Further characterization of the HSOC model demonstrated that key morphologic, molecular and immunologic features of pemphigus are being replicated. Thus, the pemphigus HSOC model is an excellent alternative to pemphigus animal model systems that are based on the transfer of (auto)antibodies.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10220749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1