Laboratory Animals最新文献_第10页

Mouse isoflurane anesthesia using the drop method. 异氟醚滴注法麻醉小鼠。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2023-12-01 Epub Date: 2023-05-05 DOI: 10.1177/00236772231169550

Maya J Bodnar, Anna S Ratuski, Daniel M Weary

{"title":"Mouse isoflurane anesthesia using the drop method.","authors":"Maya J Bodnar, Anna S Ratuski, Daniel M Weary","doi":"10.1177/00236772231169550","DOIUrl":"10.1177/00236772231169550","url":null,"abstract":"Anesthesia with isoflurane prior to carbon dioxide euthanasia is recommended as a refinement, but vaporizer access can be limited. An alternative to vaporizers is the 'drop' method, introducing a fixed volume of isoflurane into the induction chamber. Previous work suggests that isoflurane administered at a concentration of 5% via the drop method is effective but aversive to mice; lower concentrations have not been tested. We assessed mouse behavior and insensibility with induction using the drop method for isoflurane concentrations below 5%. Male Crl:CD-1 (ICR) mice (n = 27) were randomly allocated to one of three isoflurane concentrations: 1.7%, 2.7%, and 3.7%. During induction, measures of insensibility and stress-related behaviors were recorded. All mice reached a surgical plane of anesthesia, and mice exposed to higher concentrations did so more quickly; as concentrations increased from 1.7 to 2.7 and 3.7%, the time to recumbency (Least squares means ± SE: 120.5 s ± 8.1, 97.9 s ± 8.1, and 82.8 s ± 8.1, respectively), loss of righting reflex (149.1 s ± 8.5, 127.7 s ± 8.5, and 100.7 s ± 8.5, respectively), and loss of pedal withdrawal reflex (214.5 s ± 8.3, 172.2 s ± 8.3, and 146.4 s ± 8.3, respectively) all declined. Rearing was the most frequently performed stress-related behavior, and was most pronounced immediately following isoflurane administration for all treatments. Our results indicate that the drop method can be used to effectively anesthetize mice with isoflurane concentrations as low as 1.7%; future work should assess mouse aversion.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10693727/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9403162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Non-surgical external jugular vein catheterization using an ear vein access in piglets. 仔猪耳静脉非手术颈外静脉置管。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2023-12-01 Epub Date: 2023-04-19 DOI: 10.1177/00236772231167224

S K Ringer, A Schmid, M Weiss, S Ohlerth, N Spielmann, N G Clausen

{"title":"Non-surgical external jugular vein catheterization using an ear vein access in piglets.","authors":"S K Ringer, A Schmid, M Weiss, S Ohlerth, N Spielmann, N G Clausen","doi":"10.1177/00236772231167224","DOIUrl":"10.1177/00236772231167224","url":null,"abstract":"The objective of this study was to investigate the feasibility of external jugular vein catheterization through an ear vein in piglets. Forty-six sevoflurane-midazolam anaesthetized piglets were included. External jugular vein catheterization was conducted through the ear vein using the Seldinger technique. Part 1 (n = 27): optimal puncture site was based on the deltoid tuberosity as a landmark to reach the external jugular vein. The final position of the catheter was verified in 25 piglets using computer tomography. Catheterization time was recorded and patency of the catheter assessed by repeated blood sampling for up to 4 h. Part 2 (n = 19): ear vein catheterization was without taking into account any landmarks. Functionality for blood sampling was evaluated as described in part 1. Catheter advancement was possible in 25/27 and 18/19 piglets in parts 1 and 2, respectively. Median (range) time required for successful catheterization was 1.95 (1-10) min (n = 38). The deltoid tuberosity was a good landmark to reach the external jugular vein. But blood sampling was also possible through catheters ending slightly cranial to the external jugular vein. Despite successful catheter advancement, blood sampling was not possible from one catheter in each part of the study (total: two piglets). One of these catheters presented luminal damage, while the other one presented as normal after being removed from the animal. Summarizing, central vein catheterization through the ear vein was feasible in 93.5% and repeated blood sampling was possible in 89.1% of the piglets (n = 46).","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9389406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Refinement of systemic guinea pig deafening in hearing research: Sensorineural hearing loss induced by co-administration of kanamycin and furosemide via the leg veins. 听力研究中豚鼠全身性耳聋的改进:卡那霉素和速尿经腿静脉联合给药引起的感音神经性听力损失。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2023-12-01 Epub Date: 2023-04-18 DOI: 10.1177/00236772231167679

Wiebke Behrends, Daniel Ahrens, Jens P Bankstahl, Karl-Heinz Esser, Gerrit Paasche, Thomas Lenarz, Verena Scheper

{"title":"Refinement of systemic guinea pig deafening in hearing research: Sensorineural hearing loss induced by co-administration of kanamycin and furosemide via the leg veins.","authors":"Wiebke Behrends, Daniel Ahrens, Jens P Bankstahl, Karl-Heinz Esser, Gerrit Paasche, Thomas Lenarz, Verena Scheper","doi":"10.1177/00236772231167679","DOIUrl":"10.1177/00236772231167679","url":null,"abstract":"Auditory disabilities have a large impact on the human population worldwide. Research into understanding and treating hearing disabilities has increased significantly in recent years. One of the most relevant animal species in this context is the guinea pig, which has to be deafened to study several of the hearing pathologies and develop novel therapies. Applying kanamycin subcutaneously and furosemide intravenously is a long-established method in hearing research, leading to permanent hearing loss without surgical intervention at the ear. The intravenous application of furosemide requires invasive surgery in the cervical area of the animals to expose the jugular vein, since a relatively large volume (1 ml per 500 g body weight) must be injected over a period of about 2.5 min. We have established a gentler alternative by applying the furosemide by puncture of the leg veins. For this, custom-made cannula-needle devices were built to allow the vein puncture and subsequent slow injection of the furosemide. This approach was tested in 11 guinea pigs through the foreleg via the cephalic antebrachial vein and through the hind leg via the saphenous vein. Frequency-specific hearing thresholds were measured before and after the procedure to verify normal hearing and successful deafening, respectively. The novel approach of systemic deafening was successfully implemented in 10 out of 11 animals. The Vena saphena was best suited to the application. Since the animals' condition, post leg vein application, was better in comparison to animals deafened by exposure of the Vena jugularis, the postulated refinement that reduced animal stress was deemed successful.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9317674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harmonisation of education, training and continuing professional development for laboratory animal caretakers, technicians and technologists: Report of the FELASA-EFAT Working Group. 协调实验动物管理员、技术人员和技术人员的教育、培训和持续专业发展:FELASA-EFAT工作组的报告。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2023-12-01 Epub Date: 2023-06-19 DOI: 10.1177/00236772231175550

Klas Sp Abelson, Charlie Chambers, Teresa De La Cueva, Glyn Fisher, Penny Hawkins, Vasileios Ntafis, Paul F Pohlig, T Pim Rooymans, Ana I Santos

{"title":"Harmonisation of education, training and continuing professional development for laboratory animal caretakers, technicians and technologists: Report of the FELASA-EFAT Working Group.","authors":"Klas Sp Abelson, Charlie Chambers, Teresa De La Cueva, Glyn Fisher, Penny Hawkins, Vasileios Ntafis, Paul F Pohlig, T Pim Rooymans, Ana I Santos","doi":"10.1177/00236772231175550","DOIUrl":"10.1177/00236772231175550","url":null,"abstract":"Competent, confident and caring laboratory animal caretakers, technicians and technologists (LAS staff) are vital for good animal welfare, high-quality science and a secure Culture of Care. This requires high-quality education, training, supervision and continuing professional development (CPD) of LAS staff. However, there is a lack of harmonisation regarding how this education and training is conducted among European countries, and nor are there recommendations adapted to Directive 2010/63/EU. Therefore, FELASA and EFAT established a working group with the task of establishing recommendations for education, training and CPD for LAS staff. The working group established five different levels (LAS staff levels 0-4), defining the required level of competence and attitude, as well as suggesting educational requirements for reaching each level. Defining these levels should help to ensure that appropriate educational and CPD activities are in place, and to enable employers and LAS staff to determine the level and career stage attained. Furthermore, proper assessment of competencies and effective CPD schemes for all relevant staff should be established. Regulators should support this by setting standards for competence assessment and ensuring that they are consistently applied. In addition, establishments should involve the LAS staff in defining and developing the Culture of Care. The Animal Welfare Body should be involved and have oversight of education, training and CPD. These recommendations will contribute to harmonisation and increased quality of education, training and CPD, as well as provide clearer career pathways for LAS staff, helping to ensure high standards of animal welfare and science.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10693723/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10044900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FELASA Working Group report: Capture and transport of live cephalopods – recommendations for scientific purposes FELASA工作组报告:活体头足类动物的捕获和运输-用于科学目的的建议

4区农林科学

Laboratory Animals Pub Date : 2023-11-03 DOI: 10.1177/00236772231176347

Antonio V Sykes, Viola Galligioni, Juan Estefanell, Stuart Hetherington, Marco Brocca, Joao Correia, André Ferreira, Eleonora M. Pieroni, Graziano Fiorito

{"title":"FELASA Working Group report: Capture and transport of live cephalopods – recommendations for scientific purposes","authors":"Antonio V Sykes, Viola Galligioni, Juan Estefanell, Stuart Hetherington, Marco Brocca, Joao Correia, André Ferreira, Eleonora M. Pieroni, Graziano Fiorito","doi":"10.1177/00236772231176347","DOIUrl":"https://doi.org/10.1177/00236772231176347","url":null,"abstract":"On 1 January 2013, research using cephalopod molluscs, from hatchlings to adults, became regulated within Directive 2010/63/EU. There are significant difficulties in captive breeding in the great majority of currently utilised species. Thus, scientific research relies upon the use of wild-caught animals. Furthermore, live cephalopods are shared and transported between different stakeholders and laboratories across Europe and other continents. Despite existing European and national legislation, codes, guidelines and reports from independent organisations, a set of recommendations specifically addressing the requirements for the capture and transport of animals belonging to this taxon are missing. In addition, although training and development of competence for all people involved in the supply chain are essential and aim to ensure that animals do not suffer from pain, distress or lasting harm, the requirements for those capturing and transporting wild cephalopods have not been considered. This Working Group reviewed the current literature to recognise scientific evidence and the best practice, and compiled a set of recommendations to provide guidance on the ‘techniques’ to be used for the capture and transport of live cephalopods for their use in scientific procedures. In addition, we propose to (a) develop standardised approaches able to assess recommended methods and objectively quantify the impact of these processes on animals’ health, welfare and stress response, and (b) design a training programme for people attaining the necessary competence for capture and transportation of live cephalopods, as required by Directive 2010/63/EU.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135820322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FELASA recommendations for the rehoming of animals used for scientific and educational purposes. FELSA关于安置用于科学和教育目的的动物的建议。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2023-10-01 Epub Date: 2023-03-24 DOI: 10.1177/00236772231158863

Emilie Ecuer, Jackie Boxall, Annet L Louwerse, Lars F Mikkelsen, Christel Ph Moons, Mirjam Roth, Andrea M Spiri

{"title":"FELASA recommendations for the rehoming of animals used for scientific and educational purposes.","authors":"Emilie Ecuer, Jackie Boxall, Annet L Louwerse, Lars F Mikkelsen, Christel Ph Moons, Mirjam Roth, Andrea M Spiri","doi":"10.1177/00236772231158863","DOIUrl":"10.1177/00236772231158863","url":null,"abstract":"Directive 2010/63/EU of the European Parliament and the Council of 22 September 2010 states that at the end of a procedure, the most appropriate decision on the future of an animal previously used or intended for use in scientific procedures should be taken on the basis of animal welfare and potential risks to the environment. Member States may allow animals to be rehomed provided the health of the animal allows it, there is no danger to public health, animal health or the environment and if appropriate measures have been taken to safeguard the wellbeing of the animal. In countries where rehoming is permitted, it is the responsibility of the Animal Welfare Body to advise on a rehoming scheme which must include appropriate socialization in order to help facilitate successful rehoming, avoid unnecessary distress to the animals and guarantee public safety. This paper reviews the EU legislation, existing guidance, current literature and best practice to define rehoming, sets out general considerations for rehoming laboratory animals including socialization and provides practical advice on the steps required in a rehoming scheme. For those species most frequently rehomed, more detailed species-specific sections are included.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/98/01/10.1177_00236772231158863.PMC10568946.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9176265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Evaluation of the effect of tramadol, paracetamol and metamizole on the severity of experimental colitis. 曲马多、对乙酰氨基酚和安乃近对实验性结肠炎严重程度影响的评估。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2023-10-01 Epub Date: 2023-03-24 DOI: 10.1177/00236772231163957

Marianne Spalinger, Marlene Schwarzfischer, Anna Niechcial, Kirstin Atrott, Andrea Laimbacher, Paulin Jirkof, Michael Scharl

{"title":"Evaluation of the effect of tramadol, paracetamol and metamizole on the severity of experimental colitis.","authors":"Marianne Spalinger, Marlene Schwarzfischer, Anna Niechcial, Kirstin Atrott, Andrea Laimbacher, Paulin Jirkof, Michael Scharl","doi":"10.1177/00236772231163957","DOIUrl":"10.1177/00236772231163957","url":null,"abstract":"Application of dextran sodium sulfate (DSS) is often used to induce experimental colitis. Current state of the art is to refrain from the use of analgesics due to their possible interaction with the model. However, the use of analgesics would be beneficial to reduce the overall constraint imposed on the animals. Here, we analyzed the effect of the analgesics Dafalgan (paracetamol), Tramal (tramadol) and Novalgin (metamizole) on DSS-induced colitis. To study the effect of those analgesics in colitis mouse models, acute and chronic colitis was induced in female C57BL6 mice by DSS administration in the drinking water. Analgesics were added to the drinking water on days four to seven (acute colitis) or on days six to nine of each DSS cycle (chronic colitis). Tramadol and paracetamol had minor effects on colitis severity. Tramadol reduced water uptake and activity levels slightly, while mice receiving paracetamol presented with a better overall appearance. Metamizole, however, significantly reduced water uptake, resulting in pronounced weight loss. In conclusion, our experiments show that tramadol and paracetamol are viable options for the use in DSS-induced colitis models. However, paracetamol seems to be slightly more favorable since it promoted the overall wellbeing of the animals upon DSS administration without interfering with typical readouts of colitis severity.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9176268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of Concern: "Advances in research on the biology of the desert rodent Gerbillus tarabuli: a review". 关注的表达：“沙漠啮齿类动物塔拉布力的生物学研究进展：综述”。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2023-10-01 Epub Date: 2023-07-20 DOI: 10.1177/00236772231187833

引用次数: 0

Rückblick Frühjahrstagung IGTP Berlin März 2023. 审查春季会议IGTP柏林2023年3月。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2023-10-01 DOI: 10.1177/00236772231194261

引用次数: 0

Optimization of overhead enclosure monitoring in juvenile male Dunkin Hartley guinea pigs (Cavia porcellus). 幼年雄性Dunkin-Hartley豚鼠（Cavia porcellus）头顶围栏监测的优化。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2023-10-01 Epub Date: 2023-04-18 DOI: 10.1177/00236772231165810

Joel E Helbling, Alexa P Spittler, Miranda J Sadar, Kelly S Santangelo

{"title":"Optimization of overhead enclosure monitoring in juvenile male Dunkin Hartley guinea pigs (Cavia porcellus).","authors":"Joel E Helbling, Alexa P Spittler, Miranda J Sadar, Kelly S Santangelo","doi":"10.1177/00236772231165810","DOIUrl":"10.1177/00236772231165810","url":null,"abstract":"Overhead enclosure monitoring provides objective quantitative mobility measurements for animals undergoing open-field testing. Notably, protocols for testing optimization have been minimally established for the guinea pig. It is unknown whether (a) repeated exposure, (b) time-of-day, or (c) length of testing duration influence outcome parameters. We hypothesized that guinea pigs would display decreased activity following repeated exposure to the open field; heightened activity during the earliest testing period; and that 10 min would be adequate for data collection. The study was conducted in two separate phases to distinguish between enclosure habituation and time-of-day effects, respectively. Two cohorts of male Dunkin Hartley guinea pigs were allowed voluntary movement in an open-field enclosure for 14 min to quantify mobility outcomes, including total distance traveled, total time mobile, average speed while mobile, and total time spent in the shelter. For both phases, testing occurred at four different times of day, and overhead monitoring software was programmed to divide the total testing duration into 2-min bins. Habituation phase results showed time mobile and distance traveled were influenced significantly by repeat exposure, as animals were most active during the first testing event. Time-of-day phase animals spent significantly more time mobile during the earliest testing period. Interestingly, significant differences were observed across 2-min bins for the time-of-day phase but not during the habituation phase. Specifically, progressively decreased ambulatory activity was observed as testing duration increased. Thus, habituation and time-of-day should be accounted for when possible. Finally, a trial period greater than 10 min may not yield additional data.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11141528/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9317679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0