Laboratory Animals最新文献_第9页

Evaluation of the effect of tramadol, paracetamol and metamizole on the severity of experimental colitis. 曲马多、对乙酰氨基酚和安乃近对实验性结肠炎严重程度影响的评估。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2023-10-01 Epub Date: 2023-03-24 DOI: 10.1177/00236772231163957

Marianne Spalinger, Marlene Schwarzfischer, Anna Niechcial, Kirstin Atrott, Andrea Laimbacher, Paulin Jirkof, Michael Scharl

{"title":"Evaluation of the effect of tramadol, paracetamol and metamizole on the severity of experimental colitis.","authors":"Marianne Spalinger, Marlene Schwarzfischer, Anna Niechcial, Kirstin Atrott, Andrea Laimbacher, Paulin Jirkof, Michael Scharl","doi":"10.1177/00236772231163957","DOIUrl":"10.1177/00236772231163957","url":null,"abstract":"Application of dextran sodium sulfate (DSS) is often used to induce experimental colitis. Current state of the art is to refrain from the use of analgesics due to their possible interaction with the model. However, the use of analgesics would be beneficial to reduce the overall constraint imposed on the animals. Here, we analyzed the effect of the analgesics Dafalgan (paracetamol), Tramal (tramadol) and Novalgin (metamizole) on DSS-induced colitis. To study the effect of those analgesics in colitis mouse models, acute and chronic colitis was induced in female C57BL6 mice by DSS administration in the drinking water. Analgesics were added to the drinking water on days four to seven (acute colitis) or on days six to nine of each DSS cycle (chronic colitis). Tramadol and paracetamol had minor effects on colitis severity. Tramadol reduced water uptake and activity levels slightly, while mice receiving paracetamol presented with a better overall appearance. Metamizole, however, significantly reduced water uptake, resulting in pronounced weight loss. In conclusion, our experiments show that tramadol and paracetamol are viable options for the use in DSS-induced colitis models. However, paracetamol seems to be slightly more favorable since it promoted the overall wellbeing of the animals upon DSS administration without interfering with typical readouts of colitis severity.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9176268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of Concern: "Advances in research on the biology of the desert rodent Gerbillus tarabuli: a review". 关注的表达：“沙漠啮齿类动物塔拉布力的生物学研究进展：综述”。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2023-10-01 Epub Date: 2023-07-20 DOI: 10.1177/00236772231187833

引用次数: 0

Rückblick Frühjahrstagung IGTP Berlin März 2023. 审查春季会议IGTP柏林2023年3月。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2023-10-01 DOI: 10.1177/00236772231194261

引用次数: 0

Optimization of overhead enclosure monitoring in juvenile male Dunkin Hartley guinea pigs (Cavia porcellus). 幼年雄性Dunkin-Hartley豚鼠（Cavia porcellus）头顶围栏监测的优化。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2023-10-01 Epub Date: 2023-04-18 DOI: 10.1177/00236772231165810

Joel E Helbling, Alexa P Spittler, Miranda J Sadar, Kelly S Santangelo

{"title":"Optimization of overhead enclosure monitoring in juvenile male Dunkin Hartley guinea pigs (Cavia porcellus).","authors":"Joel E Helbling, Alexa P Spittler, Miranda J Sadar, Kelly S Santangelo","doi":"10.1177/00236772231165810","DOIUrl":"10.1177/00236772231165810","url":null,"abstract":"Overhead enclosure monitoring provides objective quantitative mobility measurements for animals undergoing open-field testing. Notably, protocols for testing optimization have been minimally established for the guinea pig. It is unknown whether (a) repeated exposure, (b) time-of-day, or (c) length of testing duration influence outcome parameters. We hypothesized that guinea pigs would display decreased activity following repeated exposure to the open field; heightened activity during the earliest testing period; and that 10 min would be adequate for data collection. The study was conducted in two separate phases to distinguish between enclosure habituation and time-of-day effects, respectively. Two cohorts of male Dunkin Hartley guinea pigs were allowed voluntary movement in an open-field enclosure for 14 min to quantify mobility outcomes, including total distance traveled, total time mobile, average speed while mobile, and total time spent in the shelter. For both phases, testing occurred at four different times of day, and overhead monitoring software was programmed to divide the total testing duration into 2-min bins. Habituation phase results showed time mobile and distance traveled were influenced significantly by repeat exposure, as animals were most active during the first testing event. Time-of-day phase animals spent significantly more time mobile during the earliest testing period. Interestingly, significant differences were observed across 2-min bins for the time-of-day phase but not during the habituation phase. Specifically, progressively decreased ambulatory activity was observed as testing duration increased. Thus, habituation and time-of-day should be accounted for when possible. Finally, a trial period greater than 10 min may not yield additional data.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11141528/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9317679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dental implants in large animal models with experimental systemic diseases: A systematic review. 实验性系统性疾病的大型动物模型中的牙科植入物：系统综述。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2023-10-01 Epub Date: 2023-04-06 DOI: 10.1177/00236772221124972

Flávia Gomes Matos, Anna Clara Abreu Stremel, Leandro Cavalcante Lipinski, Joni Augusto Cirelli, Fábio André Dos Santos

{"title":"Dental implants in large animal models with experimental systemic diseases: A systematic review.","authors":"Flávia Gomes Matos, Anna Clara Abreu Stremel, Leandro Cavalcante Lipinski, Joni Augusto Cirelli, Fábio André Dos Santos","doi":"10.1177/00236772221124972","DOIUrl":"10.1177/00236772221124972","url":null,"abstract":"This systematic review aims to identify and discuss the most used methodologies in pre-clinical studies for the evaluation of the implementation of dental implants in systemically compromised pigs and sheep. This study provides support and guidance for future research, as well as for the prevention of unnecessary animal wastage and sacrifice. Preferred Reporting for Systematic Reviews and Meta-Analyses (PRISMA) was used as a guideline; electronic searches were performed in PubMed, Scopus, Scielo, Web of Science, Embase, Science Direct, Brazilian Bibliography of Dentistry, Latin American and Caribbean Literature in Health Sciences, Directory of Open Access Journals, Database of Abstracts of Reviews of Effects, and gray literature until January 2022 (PROSPERO/CRD42021270119). Sixty-eight articles were chosen from the 2439 results. Most studies were conducted in pigs, mainly the Göttinger and Domesticus breeds. Healthy animals with implants installed in the jaws were predominant among the pig studies. Of the studies evaluating the effect of systemic diseases on osseointegration, 42% were performed in osteoporotic sheep, 32% in diabetic sheep, and 26% in diabetic pigs. Osteoporosis was primarily induced by bilateral ovariectomy and mainly assessed by X-ray densitometry. Diabetes was induced predominantly by intravenous streptozotocin and was confirmed by blood glucose analysis. Histological and histomorphometric analyses were the most frequently employed in the evaluation of osseointegration. The animal models presented unique methodologies for each species in the studies that evaluated dental implants in the context of systemic diseases. Understanding the most commonly used techniques will help methodological choices and the performance of future studies in implantology.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9253062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tramadol/paracetamol treatment attenuates the development of collagen antibody-induced arthritis and interferes with prednisolone treatment in mice. 曲马多/扑热息痛治疗可减轻小鼠胶原抗体诱导的关节炎的发展，并干扰泼尼松龙治疗。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2023-10-01 Epub Date: 2023-04-17 DOI: 10.1177/00236772231166029

Sanne Kroos, Mahmoud Halima, Jan Kroon, Diane van der Woude, Onno C Meijer, Maarten D van de Wal, Peternella S Verhave, Marcel Jm Schaaf, René Em Toes, Arieke Sb Kampstra

{"title":"Tramadol/paracetamol treatment attenuates the development of collagen antibody-induced arthritis and interferes with prednisolone treatment in mice.","authors":"Sanne Kroos, Mahmoud Halima, Jan Kroon, Diane van der Woude, Onno C Meijer, Maarten D van de Wal, Peternella S Verhave, Marcel Jm Schaaf, René Em Toes, Arieke Sb Kampstra","doi":"10.1177/00236772231166029","DOIUrl":"10.1177/00236772231166029","url":null,"abstract":"The collagen antibody-induced arthritis (CAIA) model is highly effective in inducing arthritis, making it an attractive model for screening therapeutic compounds such as glucocorticoids (GCs). The severity of discomfort in this model makes it desirable to administer analgesics, but it is a prerequisite that these do not interfere with the model or tested therapeutics. In the present study, we studied the effect of 1 mg/mL tramadol and 3.5 mg/mL paracetamol (TP) on CAIA in male BALB/cAnNCrl mice and the possible interference of TP analgesia with the activity of the GC drug prednisolone (Pred). Our results showed that TP abolished the Pred-induced amelioration of CAIA, as well as several other Pred-induced effects, such as the reduction in thymus weight and the increase in insulin level. This most likely results from the effects of TP on the hepatic metabolism of this drug, since it strongly increased the Cyp3a11 expression in the liver. Altogether, we conclude that TP analgesia is not suitable for the CAIA model in male BALB/cAnNCrl mice, in particular when evaluating the effects of GCs such as Pred.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9310829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating and developing sealants for the prevention of pulmonary air leakage: A systematic review of animal models. 评估和开发用于预防肺空气泄漏的密封剂：动物模型的系统综述。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2023-10-01 Epub Date: 2023-04-10 DOI: 10.1177/00236772231164873

Bob P Hermans, Steven E M Poos, Daniël I M van Dort, Jort Evers, Wilson W L Li, Erik H F M van der Heijden, Ad F T M Verhagen, Harry van Goor, Richard P G Ten Broek

{"title":"Evaluating and developing sealants for the prevention of pulmonary air leakage: A systematic review of animal models.","authors":"Bob P Hermans, Steven E M Poos, Daniël I M van Dort, Jort Evers, Wilson W L Li, Erik H F M van der Heijden, Ad F T M Verhagen, Harry van Goor, Richard P G Ten Broek","doi":"10.1177/00236772231164873","DOIUrl":"10.1177/00236772231164873","url":null,"abstract":"Sealants may provide a solution for pulmonary air leakage (PAL), but their clinical application is debatable. For sealant comparison, standardized animal models are lacking. This systematic review aims to assess methodology and quality of animal models for PAL and sealant evaluation. All animal models investigating lung sealing devices (e.g., staplers, glues, energy devices) to prevent or treat PAL were retrieved systematically from Embase, Pubmed and Web of science. Methodological study characteristics, risk of bias, reporting quality and publication bias were assessed. A total of 71 studies were included (N = 75 experiments, N = 1659 animals). Six different species and 18 strains were described; 92% of experiments used healthy animals, disease models were used in only six studies. Lesions to produce PAL were heterogenous, and only 11 studies used a previously reported technique, encompassing N = 5 unique lesions. Clinically relevant outcomes were used in the minority of studies (imaging 16%, air leak 10.7%, air leak duration 4%). Reporting quality was poor, but revealed an upward trend per decade. Overall, high risk of bias was present, and only 18.7% used a negative control group. All but one study without control groups claimed positive outcomes (95.8%), in contrast to 84.3% using positive or negative control groups, which also concluded equivocal, adverse or inconclusive outcomes. In conclusion, animal studies evaluating sealants for prevention of PAL are heterogenous and of poor reporting quality. Using negative control groups, disease models and quantifiable outcomes seem important to increase validity and relevance. Further research is needed to reach consensus for model development and standardization.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9518913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Call for submissions: Special issue on short introductory notes on biostatistics. 要求提交：关于生物统计学的简短介绍性说明的特刊。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2023-10-01 Epub Date: 2023-08-01 DOI: 10.1177/00236772231191879

Romain-Daniel Gosselin, Penelope S Reynolds

引用次数: 0

Establishment of cell lines from individual zebrafish embryos. 从斑马鱼个体胚胎中建立细胞系。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2023-10-01 Epub Date: 2023-03-09 DOI: 10.1177/00236772231157162

Nathalie Geyer, Sabrina Kaminsky, Shir Confino, Zohar Ben-Moshe Livne, Yoav Gothilf, Nicholas S Foulkes, Daniela Vallone

{"title":"Establishment of cell lines from individual zebrafish embryos.","authors":"Nathalie Geyer, Sabrina Kaminsky, Shir Confino, Zohar Ben-Moshe Livne, Yoav Gothilf, Nicholas S Foulkes, Daniela Vallone","doi":"10.1177/00236772231157162","DOIUrl":"10.1177/00236772231157162","url":null,"abstract":"With the increasing use of fish as model species for research, cell cultures derived from caudal fin explants as well as pre-hatching stage embryos have provided powerful in vitro tools that can complement or serve as an ethically more acceptable alternative to live animal experiments. The widely-used protocols to establish these lines require, as a starting point, homogeneous pools of embryos or viable adult fish which are large enough for collecting sufficient fin tissue. This excludes the use of fish lines with adverse phenotypes or lines that exhibit mortality at early developmental stages and so can only be propagated as heterozygotes. Specifically, when no visually overt mutant phenotype is detectable for identifying homozygous mutants at early embryonic stages, it is then impossible to sort pools of embryos with the same genotypes to generate cell lines from the progeny of a heterozygote in-cross. Here, we describe a simple protocol to generate cell lines on a large scale starting from individual early embryos that can subsequently be genotyped by polymerase chain reaction. This protocol should help to establish fish cell culture models as a routine approach for the functional characterization of genetic changes in fish models such as the zebrafish. Furthermore, it should contribute to a reduction of experiments which are ethically discouraged to avoid pain and distress.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9435192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Survey among FELASA members about rehoming of animals used for scientific and educational purposes. FELASA成员关于安置用于科学和教育目的的动物的调查。

IF 2.4 4区农林科学

Laboratory Animals Pub Date : 2023-10-01 Epub Date: 2023-03-24 DOI: 10.1177/00236772231153747

Christel Ph Moons, Andrea M Spiri, Jackie Boxall, Annet Louwerse, Lars F Mikkelsen, Mirjam Roth, Emilie Ecuer

{"title":"Survey among FELASA members about rehoming of animals used for scientific and educational purposes.","authors":"Christel Ph Moons, Andrea M Spiri, Jackie Boxall, Annet Louwerse, Lars F Mikkelsen, Mirjam Roth, Emilie Ecuer","doi":"10.1177/00236772231153747","DOIUrl":"10.1177/00236772231153747","url":null,"abstract":"Rehoming is an important fate, which should be considered for animals used for scientific and educational purposes, and which is highlighted in the European Directive 2010/63 EU. In 2018, the Federation of European Laboratory Animal Science Associations (FELASA) convened a working group to review current literature and identify existing practices with the aim of issuing general recommendations on the rehoming of research animals. In order to understand the number and species of animals being rehomed and which species and information to include in the recommendations, the working group launched a survey that was distributed among FELASA members, yielding 97 valid records for analysis. Most respondents of the survey considered the rehoming of cats, dogs, mice, rats, rabbits, pigs and minipigs. The most important issues reported by the respondents were related to availability/suitability of animals, availability of adopters and legal issues. Based on the data and information collected in this survey, the working group decided on the format and content of the future recommendations: a first section containing a general protocol for rehoming, addressing the issues raised by the respondents, and a second section containing species-specific information and advice about cats, dogs, small prey mammals, equines, primates, camelids and minipigs.","PeriodicalId":18013,"journal":{"name":"Laboratory Animals","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/bc/d6/10.1177_00236772231153747.PMC10568943.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9176264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3