Half the price, twice the gain: How to simultaneously decrease animal numbers and increase precision with good experimental design.

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES
Laboratory Animals Pub Date : 2024-10-01 Epub Date: 2024-09-24 DOI:10.1177/00236772241260905
Servan Luciano Grüninger, Florian Frommlet
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引用次数: 0

Abstract

Animal research often involves experiments in which the effect of several factors on a particular outcome is of scientific interest. Many researchers approach such experiments by varying just one factor at a time. As a consequence, they design and analyze the experiments based on a pairwise comparison between two groups. However, this approach uses unreasonably large numbers of animals and leads to severe limitations in terms of the research questions that can be answered. Factorial designs and analyses offer a more efficient way to perform and assess experiments with multiple factors of interest. We will illustrate the basic principles behind these designs, discussing a simple example with only two factors before suggesting how to design and analyze more complex experiments involving larger numbers of factors based on multiway analysis of variance.

价格减半,收益加倍:如何通过良好的实验设计同时减少动物数量和提高精确度。
动物研究经常涉及到一些实验,在这些实验中,多个因素对某一特定结果的影响会引起科学兴趣。许多研究人员在进行此类实验时,每次只改变一个因素。因此,他们根据两组之间的配对比较来设计和分析实验。然而,这种方法使用了大量不合理的动物,导致在回答研究问题方面受到严重限制。因子设计和分析提供了一种更有效的方法来执行和评估具有多个相关因子的实验。我们将说明这些设计背后的基本原理,先讨论一个只有两个因子的简单例子,然后建议如何根据多向方差分析设计和分析涉及更多因子的更复杂实验。
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来源期刊
Laboratory Animals
Laboratory Animals 生物-动物学
CiteScore
4.90
自引率
8.30%
发文量
64
审稿时长
6-12 weeks
期刊介绍: The international journal of laboratory animal science and welfare, Laboratory Animals publishes peer-reviewed original papers and reviews on all aspects of the use of animals in biomedical research. The journal promotes improvements in the welfare or well-being of the animals used, it particularly focuses on research that reduces the number of animals used or which replaces animal models with in vitro alternatives.
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